Microbial ecological bacterial fertilizer for increasing sugar content of silage corn and preparation method of microbial ecological bacterial fertilizer

Abstract

The invention relates to the technical field of fertilizers, in particular to a microbial ecological bacterial fertilizer for increasing sugar content of silage corn and a preparation method of the microbial ecological bacterial fertilizer. Then inoculating into a solid matrix for fermentation to obtain fermentation liquor; filtering the fermentation liquor to remove impurities, and mixing to obtain an actinomycete fungicide; thoroughly decomposed sheep manure and sucrose residues are uniformly mixed to obtain a compost substrate, and the compost substrate is subjected to compost fermentation to obtain organic fertilizer; chopping cleaned brown algae, and stirring the chopped brown algae with deionized water to obtain a seaweed extracting solution; filtering the seaweed extracting solution to remove impurities, and concentrating to obtain a seaweed extract concentrated solution; adding the actinomycetes fungicide, the corn flour, the soybean amino acid powder, the organic fertilizer, the chemical fertilizer and the trace elements into a double-shaft mixer to obtain a main mixed material; granulating and drying the main mixed material to obtain dry particles, and spraying a polyglutamic acid film on the surfaces of the dry particles to obtain the microbial ecological bacterial fertilizer. The sugar content of the silage corn is increased.

Description

Technical Field

[0001] The present invention relates to the technical field of fertilizers, and particularly relates to a microbial ecological fertilizer for increasing the sugar content of silage corn and a preparation method thereof. Background Art

[0002] Silage corn is an important forage crop, which has the characteristics of good palatability and easy digestibility. During the ensiling process, the sugar in the corn is an important substrate for lactic acid bacteria fermentation. The sugar content directly affects the quality and storage time of silage feed. Actinomycetes are a class of microorganisms with strong decomposition ability, which can decompose organic substances in the soil and release nutrients required by plants. Actinomycetes can significantly improve the physical and chemical properties of the soil and increase soil fertility. Seaweed extracts are rich in various bioactive substances, which can enhance the stress resistance of plants, promote the absorption of nutrients by plants, and thus improve the quality and yield of crops.

[0003] Chinese Patent Application Publication No.: CN108085274A discloses a microbial fertilizer for promoting the nutrient absorption of corn and a preparation method thereof. This fertilizer is mainly made from Agrobacterium rhizogenes A4, R1205, R1000, R1601, R1022, and 15834. Agrobacterium rhizogenes has the effect of promoting the rooting and growth of plants, and thus promotes the nutrient absorption of plants, which has an important impact on plant growth and development. Therefore, a microbial fertilizer is made from an Agrobacterium rhizogenes bacterial liquid with an OD value of 0.4 - 0.6, an organic matter composition, and ordinary loam soil, and is applied to corn seedlings at the 2 - 3 compound leaf stage, which can stimulate the root growth of corn and increase the root absorption area, improve the nutrient absorption efficiency of corn, mainly promote the absorption of N and P by corn, and thus promote the growth and development of corn, overcoming the problems of low quality and low yield in corn production. However, there are the following problems in the prior art: The prior art uses Agrobacterium rhizogenes to promote the absorption of N and P by corn, without performing refined treatment on the preparation of the microbial ecological fertilizer, resulting in the problem of low sugar storage in silage corn. Summary of the Invention

[0004] Therefore, the present invention provides a microbial ecological fertilizer for increasing the sugar content of silage corn and a preparation method thereof, so as to overcome the problem in the prior art that the preparation of the microbial ecological fertilizer is not refined, resulting in low sugar storage in silage corn.

[0005] To achieve the above object, the present invention provides a microbial ecological fertilizer for increasing the sugar content of silage corn and a preparation method thereof, including:

[0006] Streptomyces pactum and Streptomyces rochei are separately inoculated into two activation media for cultivation to obtain a first spore suspension of the strain and a second spore suspension of the strain respectively;

[0007] The first spore suspension of the strain and the second spore suspension of the strain are separately inoculated into a solid substrate for fermentation to obtain a first fermentation broth and a second fermentation broth respectively;

[0008] The first fermentation broth and the second fermentation broth are filtered to remove impurities and then mixed to obtain an actinomycetes agent;

[0009] The well-rotted sheep manure is mixed evenly with sucrose residue at a preset mass percentage to obtain a compost substrate, and the compost substrate is subjected to compost fermentation for a preset time to obtain organic fertilizer;

[0010] The cleaned brown algae are chopped and added to deionized water in a stirring container, and a seaweed extract is obtained in the stirring container under preset stirring conditions;

[0011] The seaweed extract is filtered to remove impurities and then transported to a concentration device, and a concentrated seaweed extract is obtained in the concentration device under preset concentration conditions;

[0012] The actinomycetes agent, corn flour and soybean amino acid powder are added to a double-shaft mixer, and a premixed material is obtained in the double-shaft mixer under preset premixing conditions. The organic fertilizer, chemical fertilizer and trace elements are added to the premixed material, and a main mixed material is obtained in the double-shaft mixer under preset main mixing conditions;

[0013] The main mixed material is granulated and dried to obtain dry granules, and a polyglutamic acid film with a concentration of 0.5% is sprayed on the surface of the dry granules to obtain a microbial ecological fertilizer.

[0014] Further, the weight ratio of the activation medium is 4% glucose, 4% yeast extract powder, 10% malt extract powder, 6% peptone, 14% agar, and 62% distilled water. The pH of the activation medium is 7.2, the temperature is 25 °C, the culture time is 48 h, and the shaker speed is 180 rpm.

[0015] Further, the mass ratio concentration of the solid substrate is wheat bran: diatomite = 3:1, and the fermentation conditions are set as the temperature of 28 °C - 30 °C and the relative humidity of 60 °C - 70 °C.

[0016] Further, the mixing conditions for mixing the first fermentation broth and the second fermentation broth are that the temperature range is set at 25 °C - 30 °C, the pH is 6.5 - 7.5, the mixing ratio is 7:3 by volume, the stirring speed is 100 rpm - 150 rpm, the mixing time is 2 h - 4 h, and sterile air with a saturation of 20% - 40% is introduced.

[0017] Further, the preset mass percentage of the sugarcane residue is 2%, the mixing ratio of the decomposed sheep manure to the sugarcane residue is 4:1 by mass, the preset duration is set to 30 - 35 days, and the pH of the organic fertilizer is 6.8 - 7.6.

[0018] Further, the size of the shredded brown algae is a square block with a side length of 0.5 cm. The mixing ratio of the brown algae to the deionized water is 1:10 by mass. The preset stirring conditions are that the stirring speed is set to 200 rpm - 250 rpm, the temperature is set to 50°C - 60°C, and the time is set to 1.5 h - 2 h.

[0019] Further, the preset concentration conditions are that the concentration temperature is set to 40°C - 50°C, the vacuum degree is set to -0.08 MPa - 0.1 MPa, and the concentration time is set to 1 h - 1.5 h.

[0020] Further, the mixing ratio of the actinomycete agent, the corn flour, and the soybean amino acid powder is 1:3:6 by weight. The preset premixing conditions are that the premixing speed is set to 20 rpm - 30 rpm, the temperature is set to 25°C - 30°C, and the time is set to 10 min - 15 min.

[0021] Further, the mixing ratio of the premixed material, the organic fertilizer, the chemical fertilizer, and the trace elements is 4:3:2:1 by weight. The preset main mixing conditions are that the main mixing speed is set to 40 rpm - 50 rpm, the temperature is set to 25°C - 30°C, and the time is set to 20 min - 30 min.

[0022] On the other hand, the present invention also provides a microbial ecological bacterial fertilizer for increasing the sugar content of silage corn. The microbial ecological bacterial fertilizer is composed of the following raw materials: Streptomyces pactum, Streptomyces rochei, decomposed sheep manure, sugarcane residue, concentrated seaweed extract, corn flour, soybean amino acid powder, organic fertilizer, chemical fertilizer, trace elements, and polyglutamic acid film.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows: By selecting and activating Streptomyces pactum and Streptomyces rochei, combining solid substrate fermentation technology, and applying compost substrate and seaweed extract, and supplemented by a mixing process, through granulation drying and spraying with polyglutamic acid film, the microbial ecological bacterial fertilizer is prepared, which improves the quality and stability of the bacterial fertilizer, can loosen the soil structure, improve the soil microenvironment, provides a more relaxed and fertile growth space for the root and stem growth of silage corn, and the root and stem of silage corn can more effectively absorb water and nutrients in the soil, thereby increasing the sugar content of silage feed.

[0024] Furthermore, by activating and culturing Streptomyces pactum and Streptomyces rochei, the present invention ensures the high activity and high efficiency of the strains, improves the survival rate of the strains, indirectly promotes the increase of soil microbial diversity, helps to loosen the soil structure, and provides a broader space for the root and stem of silage corn to absorb sugar.

[0025] Furthermore, the present invention inoculates the activated spore suspension of the strain into a solid matrix for fermentation, and prepares a bacterium agent rich in actinomycetes by controlling the fermentation conditions, retains the excellent traits of the strain, has good soil improvement ability, can promote the formation of soil aggregate structure, further loosen the soil, and thus helps silage corn absorb more sugar.

[0026] Furthermore, the present invention mixes decomposed sheep manure and sugarcane bagasse evenly to prepare a compost matrix, and prepares organic fertilizer through the compost fermentation process. The addition of sugarcane bagasse increases the carbon-nitrogen ratio of the compost matrix and promotes the reproduction and activity of microorganisms.

[0027] Furthermore, the present invention extracts effective components from brown algae to prepare a concentrated seaweed extract, enriches the nutrient composition of the bacterial fertilizer. The plant growth regulating substances and trace elements in the seaweed extract can significantly enhance the growth potential of plants, and indirectly promote the absorption and utilization of sugar by the roots and stems of silage corn through multiple ways such as promoting root development and enhancing photosynthesis efficiency.

[0028] Furthermore, the present invention is prepared by mixing an actinomycetes bacterium agent, corn flour, soybean amino acid powder, organic fertilizer, chemical fertilizer and trace elements, ensuring the balance and comprehensiveness of the nutrients of the bacterial fertilizer, maximizing the retention of the microbial activity in the bacterial fertilizer, improving the overall quality of the bacterial fertilizer, enhancing the overall soil optimization ability of the bacterial fertilizer, and creating a more fertile and suitable growth matrix for silage corn.

[0029] Furthermore, the present invention processes the main mixed materials into easily applied granular form, and ensures the stability and persistence of the bacterial fertilizer through drying treatment. A polyglutamic acid film with a concentration of 0.5% is sprayed on the surface of the dried granules, improving the water retention and fertilizer retention properties of the bacterial fertilizer. The polyglutamic acid film can slowly release nutrients, provide a continuous and stable nutrient supply for silage corn, and at the same time promote the activity of soil microorganisms, thereby increasing the sugar content of silage corn. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 is a flowchart of the preparation method of the microbial ecological bacterial fertilizer for increasing the sugar content of silage corn in the embodiment of the present invention;

[0031] Figure 2 is a flowchart of the cultivation process of Streptomyces pactum and Streptomyces rochei in the embodiment of the present invention;

[0032] Figure 3 This is a flowchart of the preparation process of the seaweed extract in the embodiments of the present invention;

[0033] Figure 4 This is a flowchart of the preparation process of the main mixed material in the embodiments of the present invention. Specific embodiments

[0034] In order to make the objectives and advantages of the present invention clearer, the present invention will be further described below in conjunction with embodiments; it should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0035] The preferred embodiments of the present invention will be described below with reference to the accompanying drawings. Those skilled in the art should understand that these embodiments are only used to explain the technical principles of the present invention and do not limit the protection scope of the present invention.

[0036] It should be noted that the data in this embodiment are all obtained through comprehensive analysis and evaluation of the historical test data and corresponding historical test results in the three months before this test by the present invention. Those skilled in the art can understand that the determination method of the present invention for a single above-mentioned parameter can be to select the value with the highest proportion according to the data distribution as the preset standard parameter, use weighted summation to obtain the value as the preset standard parameter, substitute each historical data into a specific formula and use the value obtained by this formula as the preset standard parameter or other selection methods, as long as it meets the requirement that the present invention can clearly define different specific situations in the single determination process through the obtained values.

[0037] Please refer to Figure 1 the flowchart of the preparation method of the microbial ecological bacterial fertilizer for increasing the sugar content of silage corn shown:

[0038] The preparation method of the microbial ecological bacterial fertilizer for increasing the sugar content of silage corn in the embodiments of the present invention includes:

[0039] Step S1, inoculating Streptomyces pactum and Streptomyces rochei into two activation media for cultivation respectively to obtain a first strain spore suspension and a second strain spore suspension;

[0040] Step S2, inoculating the first strain spore suspension and the second strain spore suspension into a solid matrix for fermentation respectively to obtain a first fermentation broth and a second fermentation broth;

[0041] Step S3, filtering and removing impurities from the first fermentation broth and the second fermentation broth and then mixing them to obtain an actinomycetes bacterium agent;

[0042] Step S4, mixing the decomposed sheep manure and sucrose residue with a preset mass percentage evenly to obtain a compost matrix, and performing compost fermentation on the compost matrix for a preset time to obtain an organic fertilizer;

[0043] Step S5: After chopping the cleaned brown algae, add it and deionized water into a stirring container, and obtain an algal extract in the stirring container through preset stirring conditions;

[0044] Step S6: Filter and remove impurities from the algal extract and then transport it to a concentration device, and obtain a concentrated algal extract in the concentration device under preset concentration conditions;

[0045] Step S7: Add the actinomycete agent, corn flour, and soybean amino acid powder into a double-shaft mixer, and obtain a premixed material in the double-shaft mixer under preset premixing conditions. Then add the organic fertilizer, chemical fertilizer, and trace elements into the premixed material, and obtain a main mixed material in the double-shaft mixer under preset main mixing conditions;

[0046] Step S8: Granulate and dry the main mixed material to obtain dry granules, and spray a polyglutamic acid film with a concentration of 0.5% on the surface of the dry granules to obtain a microbial ecological bacterial fertilizer.

[0047] Specifically, the present invention selects and activates Streptomyces pactum and Streptomyces rochei, combines solid substrate fermentation technology, and the application of compost substrate and algal extract, and is supplemented with a mixing process. Through granulation drying and polyglutamic acid film spraying, a microbial ecological bacterial fertilizer is prepared, which improves the quality and stability of the bacterial fertilizer, can loosen the soil structure, improve the soil microenvironment, provides a more loose and fertile growth space for the root and stem growth of silage corn, and the root and stem of silage corn can absorb water and nutrients in the soil more effectively, thereby increasing the sugar content of silage feed.

[0048] In the embodiment of the present invention, the strain number of Streptomyces pactum is ACCC 40098, and the effective viable count ≥ 2×10^9 CFU / g. The strain number of Streptomyces rochei is CGMCC 4.1765, and the effective viable count ≥ 1.5×10^9 CFU / g.

[0049] Specifically, the weight ratio of the activation medium is glucose 4%, yeast extract powder 4%, malt extract powder 10%, peptone 6%, agar 14%, and distilled water 62%.

[0050] Specifically, adjust the activation medium to 7.2, and place the activation medium in a high-pressure sterilizer at 121°C for 30 minutes. The above process is a conventional process and will not be elaborated here.

[0051] Please refer to Figure 2 the flowchart of the culture process of Streptomyces pactum and Streptomyces rochei shown in:

[0052] Specifically, the culture process of Streptomyces pactum and Streptomyces rochei is as follows: Step S11: Inoculate the Streptomyces pactum and the Streptomyces rochei into a shaker at a temperature of 25°C and a rotation speed of 180 rpm for 48 h; Step S12: After the culture is completed, transfer the Streptomyces pactum bacterial liquid and the Streptomyces rochei bacterial liquid into sterile centrifuge tubes respectively, centrifuge at a speed of 4000 rpm for 10 min, and collect the precipitates of the two kinds of bacteria; Step S13: Add sterile normal saline with a volume 5 times that of the bacterial precipitate volume to the precipitates of the two kinds of bacteria respectively, and use sterile glass beads to shake at a speed of 200 rpm for 30 min to disperse the spore masses in the precipitates of the two kinds of bacteria; Step S14: Filter the mycelium fragments with sterile absorbent cotton to obtain a pure first-strain spore suspension and a second-strain spore suspension.

[0053] Specifically, by activating and culturing Streptomyces pactum and Streptomyces rochei, the present invention ensures the high activity and high efficiency of the strains, improves the survival rate of the strains, indirectly promotes the increase of soil microbial diversity, helps to loosen the soil structure, and provides a wider space for sugar absorption for the root and stem growth of silage corn.

[0054] Specifically, the mass ratio of the solid matrix is wheat bran: diatomite = 3:1. Place the solid matrix in a high-pressure sterilizer at 121°C for 30 minutes and then cool it to room temperature. The above process is a conventional process and will not be elaborated here.

[0055] Specifically, evenly spray the first-strain spore suspension and the second-strain spore suspension with an inoculation amount of 5% onto the surface of the sterilized solid matrix respectively, stir while spraying to ensure the uniform distribution of the spore suspension, set the fermentation conditions as a temperature of 28°C to 30°C and a relative humidity of 60°C to 70°C. After 5 days of fermentation, transfer the fermented matrix into sterile gauze respectively and squeeze out the liquid part to obtain the first fermentation liquid and the second fermentation liquid.

[0056] Specifically, mix the first fermentation liquid and the second fermentation liquid and pour them into a mixing tank at a mixing ratio of 7:3 by volume to obtain an actinomycetes agent. The temperature range of the mixing tank is set at 25°C to 30°C, the pH is set at 6.5 to 7.5, start the stirrer, set the stirring speed at 100 rpm to 150 rpm, and introduce sterile air with a saturation of 20% to 40% during the mixing process.

[0057] Specifically, the present invention inoculates the activated strain spore suspension into the solid matrix for fermentation, prepares an actinomycetes-rich agent by controlling the fermentation conditions, retains the excellent traits of the strain, has good soil improvement ability, can promote the formation of soil aggregate structure, further loosen the soil, and thus helps silage corn absorb more sugar.

[0058] Specifically, the decomposed sheep manure is mixed evenly with sucrose residue at a preset mass percentage to obtain a compost substrate. Decomposed sheep manure with a decomposition degree ≥ 90%, a C / N ratio of 18 - 22, and an organic matter content ≥ 45% is selected. The mass ratio of the decomposed sheep manure to the sucrose residue is 4:1, and the addition amount of the sucrose residue is 2% of the total mass of the compost substrate. The decomposed sheep manure and the sucrose residue are added into a trough type turning machine in proportion and mixed evenly. During the mixing process, water is sprayed to keep the water content of the mixture at 50% - 60%.

[0059] Specifically, the mixed compost substrate is piled up in a compost yard. The height of the compost substrate pile is 1.5 m - 2 m, and the width is 2 m - 3 m. The shape of the compost substrate is trapezoidal. During the fermentation process of the compost substrate, the compost temperature is 50°C - 65°C, the water content of the compost substrate is 50% - 60%, during the fermentation process, the pH value is 6.8 - 7.6, and the fermentation time is 30 - 35 days.

[0060] Specifically, the present invention prepares a compost substrate by mixing decomposed sheep manure and sucrose residue evenly and prepares organic fertilizer through a compost fermentation process. The addition of sucrose residue increases the carbon-nitrogen ratio of the compost substrate and promotes the reproduction and activity of microorganisms.

[0061] Please refer to Figure 3 the flowchart of the preparation process of the seaweed extract shown:

[0062] Specifically, the preparation process of the seaweed extract is as follows. Step S21: The brown algae are rinsed clean with clear water to remove the sediment and impurities on the surface, and the brown algae are chopped into square blocks with a side length of 0.5 cm using a chopper; Step S22: The chopped brown algae are mixed with deionized water at a mass ratio of 1:10 to obtain a mixed material; Step S23: The mixed material is added into a stirrer, the stirring speed of the stirrer is 200 rpm - 250 rpm, the temperature is set at 50°C - 60°C, and the time is set at 1.5 h - 2 h; Step S24: The stirred mixture is filtered to remove residues to obtain the seaweed extract.

[0063] Specifically, the seaweed extract is transported to a vacuum concentrator. The settings of the vacuum concentrator are 40°C - 50°C, the vacuum degree is set at -0.08 MPa - 0.1 MPa, and the concentration time is set at 1 h - 1.5 h. The seaweed extract is concentrated to 1 / 5 of the original volume, and the original volume is the volume of the seaweed extract obtained after filtering to remove residues.

[0064] Specifically, the present invention enriches the nutrient composition of the microbial fertilizer by extracting active ingredients from brown algae and preparing a concentrated seaweed extract. The plant growth regulating substances and trace elements in the seaweed extract can significantly enhance the growth potential of plants. Indirectly, the absorption and utilization of sugars by the roots and stems of silage corn are promoted through multiple ways such as promoting root development and enhancing photosynthesis efficiency.

[0065] Please refer to Figure 4 the flowchart of the main mixed material preparation process shown:

[0066] Specifically, in the preparation process of the main mixed material, step S31: Add the actinomycete inoculant, corn flour, and soybean amino acid powder into a double-shaft mixer in a weight ratio of 1:3:6 for mixing; step S32: The preset pre-mixing conditions of the double-shaft mixer are that the pre-mixing speed is set to 20 rpm - 30 rpm, the temperature is set to 25°C - 30°C, and mix for 10 min - 15 min to obtain a pre-mixed material; step S33: Then mix the pre-mixed material with the organic fertilizer, chemical fertilizer, and trace elements in a mixing ratio of 4:3:2:1 by weight; step S34: The preset main mixing conditions of the double-shaft mixer are that the main mixing speed is set to 40 rpm - 50 rpm, the temperature is set to 25°C - 30°C, and mix for 20 min - 30 min to obtain the main mixed material.

[0067] In the embodiment of the present invention, the chemical fertilizer is urea with a mass ratio of 30%, superphosphate with a mass ratio of 20%, and potassium sulfate with a mass ratio of 50%. The trace elements are chelated Zn-EDTA with a mass ratio of 50%, borax with a mass ratio of 30%, and ferrous sulfate with a mass ratio of 20%.

[0068] Specifically, the present invention is prepared by mixing actinomycete inoculant, corn flour, soybean amino acid powder, organic fertilizer, chemical fertilizer, and trace elements, ensuring the balance and comprehensiveness of the nutrients in the microbial fertilizer, maximizing the retention of the microbial activity in the microbial fertilizer, improving the overall quality of the microbial fertilizer, enhancing the overall soil optimization ability of the microbial fertilizer, and creating a more fertile and suitable growth substrate for silage corn.

[0069] Specifically, add the main mixed material into a granulator. The rotation speed of the granulator is 20 rpm - 30 rpm, the inclination angle of the granulator is set to 45° - 60°, the pressure is set to 2 MPa - 3 MPa, and the pore diameter is set to 2 mm - 4 mm. Uniformly spread the wet granules after granulation on a drying tray. Set the drying temperature to 60°C - 70°C, and after drying for 30 min - 45 min, obtain dried granules. Spray a polyglutamic acid film with a concentration of 0.5% on the surface of the dried granules to obtain the microbial ecological fertilizer.

[0070] Specifically, the present invention processes the main mixed materials into granular form that is easy to apply, and through drying treatment, ensures the stability and persistence of the bacterial fertilizer. A polyglutamic acid film with a concentration of 0.5% is sprayed on the surface of the dried granules, improving the water retention and fertilizer retention properties of the bacterial fertilizer. The polyglutamic acid film can slowly release nutrients, providing a continuous and stable nutrient supply for silage corn, and at the same time promoting the activity of soil microorganisms, thereby increasing the sugar content of silage corn.

[0071] On the other hand, an embodiment of the present invention provides a microbial ecological bacterial fertilizer for increasing the sugar content of silage corn. The microbial ecological bacterial fertilizer is composed of the following raw materials: Streptomyces pactum, Streptomyces rochei, well-rotted sheep manure, sugarcane bagasse, concentrated seaweed extract, corn flour, soybean amino acid powder, organic fertilizer, chemical fertilizer, trace elements, and polyglutamic acid film.

[0072] Example 1:

[0073] During the implementation process, the temperature of the solid matrix is set to 28°C, and the relative humidity is set to 60°C; the temperature of the mixture of the first fermentation broth and the second fermentation broth is set to 25°C, the pH is set to 6.5, the stirring speed is set to 100 rpm, the mixing time is set to 2 h, and sterile air with a saturation of 20% is introduced; the fermentation duration of well-rotted sheep manure and sugarcane bagasse is set to 30 days, and the pH of the organic fertilizer is set to 6.8; the stirring speed of brown algae and deionized water is set to 200 rpm, the temperature is set to 50°C, and the time is set to 1.5 h; the concentration temperature is set to 40°C, the vacuum degree is set to 0.08 MPa, and the concentration time is set to 1 h. The pre-mixing speed of the actinomycete agent with corn flour and soybean amino acid powder is set to 20 rpm, the temperature is set to 25°C, and the time is set to 10 min; the main mixing speed of the pre-mixed materials with organic fertilizer, chemical fertilizer, and trace elements is set to 40 rpm, the temperature is set to 25°C, and the time is set to 20 min.

[0074] The raw materials required for preparing 1 kg of the microbial ecological bacterial fertilizer are as follows:

[0075] (1) After activation culture, fermentation, and mixing of Streptomyces pactum and Streptomyces rochei, 38 g of actinomycetes are obtained;

[0076] (2) 228 g of well-rotted sheep manure and 57 g of sugarcane bagasse are composted and fermented to obtain organic fertilizer;

[0077] (3) 13.6 g of brown algae and 136.4 g of deionized water are stirred and concentrated to obtain concentrated seaweed extract;

[0078] (4) Premix 38 g of actinomycetes inoculant, 114 g of corn flour, and 228 g of soybean amino acid powder to obtain a premixed material, and add organic fertilizer, chemical fertilizer, and trace elements to the premixed material to obtain a main mixed material. Among them, the chemical fertilizer includes 57 g of urea, 38 g of superphosphate, and 95 g of potassium sulfate, and the trace elements include 47.5 g of chelated Zn-EDTA, 28.5 g of borax, and 19 g of ferrous sulfate.

[0079] (5) Spray 0.1 g of polyglutamic acid film onto the dried granules to obtain microbial ecological bacterial fertilizer.

[0080] Table 1.1 shows the physical and chemical properties of the prepared microbial ecological bacterial fertilizer.

[0081] Item Experimental parameters Standard parameters Control parameters Number of viable bacteria (CFU / g) 3.8×10^9 1.2×10^9 ≥2×10^9 Organic matter content (%) 48.2 38.5 ≥45 Water content (%) 7.5 9.2 ≤8 pH value 7.1 6.3 6.8～7.6

[0082] The standard parameters are the physical and chemical property parameters of ordinary microbial ecological bacterial fertilizer, and the control parameters are the physical and chemical property parameters of organic fertilizer.

[0083] Table 1.2 designs experiments with different bacterial fertilizer formulas, strain ratios, and application rates.

[0084]

[0085] Table 1.3 shows the comparison of sugar content in silage corn and soil properties after the experiment.

[0086] Experiment name Sugar content (%) Soil porosity (%) Organic matter (%) Microbial diversity EG 18.6 42.5 4.2 8.2 CG 14.3 35.2 2.8 5.6 PG 16.1 38.7 3.5 6.8 SG 17.4 40.1 3.9 7.5 AG1 15.8 36.9 3.1 6.2 AG2 18.6 42.5 4.2 8.2 AG3 19.2 43.8 4.5 8.5

[0087] Table 1.4 shows the comparison of growth indicators of silage corn after the experiment.

[0088]

[0089]

[0090] Specifically, based on the comparison results of Tables 1.2, 1.3, and 1.4, the following conclusions are drawn: (1) When comparing some of the formulation groups (PG) with the experimental group (EG), it is found that after removing the seaweed extract, the sugar content, growth indicators, and improvement effect of soil properties of the silage corn all decrease. This shows that the seaweed extract plays an important auxiliary role in the biofertilizer formulation, promoting the growth of silage corn and soil improvement by providing nutrients or regulating microbial activity, etc.; (2) When comparing the strain ratio group (SG) with the experimental group (EG), it is found that although there is still a certain promoting effect after adjusting the strain ratio to 1:1, it is not as good as the experimental group in terms of sugar content, growth indicators, and soil property improvement. This indicates that the strain ratio (7:3) has an important impact on the biofertilizer effect and is more conducive to the synergistic effect between strains, thus exerting a better effect; (3) The results of the application rate group (AG) show that when the application rate of the biofertilizer increases within a certain range, the improvement effects of the sugar content, growth indicators, and soil properties of the silage corn also increase; (4) The control group (CG) only uses ordinary organic fertilizer, and its various indicators are lower than those of the experimental group and other treatment groups. The effect of ordinary organic fertilizer is not as good as that of the microbial ecological biofertilizer added with Streptomyces pactum, Streptomyces rochei, and seaweed extract.

[0091] Example 2

[0092] During the implementation process, the temperature of the solid matrix is set at 30 °C, and the relative humidity is set at 70 °C; the temperature for mixing the first fermentation broth and the second fermentation broth is set at 30 °C, the pH is set at 7.5, the stirring speed is set at 150 rpm, the mixing time is set at 4 h, and sterile air with a saturation of 40% is introduced; the fermentation duration of the decomposed sheep manure and sucrose residue is set at 35 days, and the pH of the organic fertilizer is set at 7.6; the stirring speed of the brown algae and deionized water is set at 250 rpm, the temperature is set at 60 °C, and the time is set at 2 h; the concentration temperature is set at 50 °C, the vacuum degree is set at 0.1 MPa, the concentration time is set at 1.5 h, the pre-mixing speed of the actinomycete agent with corn flour and soybean amino acid powder is set at 30 rpm, the temperature is set at 30 °C, and the time is set at 15 min; the main mixing speed of the pre-mixed material with the organic fertilizer, chemical fertilizer, and trace elements is set at 50 rpm, the temperature is set at 30 °C, and the time is set at 30 min.

[0093] The raw materials required to prepare 1 kg of the microbial ecological biofertilizer are as follows:

[0094] (1) After activating, culturing, and fermenting Streptomyces pactum and Streptomyces rochei and then mixing them, 38 g of actinomycetes are obtained;

[0095] (2) Compost fermentation is carried out on 228 g of decomposed sheep manure and 57 g of sucrose residue to obtain organic fertilizer;

[0096] (3) Stir and concentrate 13.6 g of brown algae and 136.4 g of deionized water to obtain a concentrated seaweed extract solution;

[0097] (4) Pre-mix 38 g of actinomycete inoculant, 114 g of corn flour and 228 g of soybean amino acid powder to obtain a pre-mixed material, and add organic fertilizer, chemical fertilizer and trace elements to the pre-mixed material to obtain a main mixed material, wherein the chemical fertilizer includes 57 g of urea, 38 g of superphosphate and 95 g of potassium sulfate, and the trace elements include 47.5 g of chelated Zn-EDTA, 28.5 g of borax and 19 g of ferrous sulfate.

[0098] (5) Spray 0.1 g of polyglutamic acid film onto the dry particles to obtain the microbial ecological bacterial fertilizer.

[0099] Table 2.1 shows the physical and chemical properties of the prepared microbial ecological bacterial fertilizer.

[0100] Item Experimental parameters Standard parameters Control parameters Number of viable bacteria (CFU / g) 3.9×10^9 1.2×10^9 ≥2×10^9 Organic matter content (%) 48.5 38.5 ≥45 Water content (%) 7.2 9.2 ≤8 pH value 7.1 6.3 6.8～7.6

[0101] The standard parameters are the physical and chemical property parameters of ordinary microbial ecological bacterial fertilizer, and the control parameters are the physical and chemical property parameters of organic fertilizer.

[0102] Table 2.2 designs experiments with different bacterial fertilizer formulas, strain ratios and application rates.

[0103]

[0104] Table 2.3 shows the comparison of sugar content of silage corn and soil properties after the experiment.

[0105]

[0106]

[0107] Table 2.4 shows the comparison of growth indexes of silage corn after the experiment.

[0108] Experiment name Plant height (cm) Stem diameter (mm) Chlorophyll (SPAD) Root dry weight (g) EG 295 34 50.5 49.2 CG 235 26 39.2 28.7 PG 272 30 44.3 37.8 SG 285 32 47.6 43.5 AG1 255 28 41.8 33.6 AG2 295 34 50.5 49.2 AG3 305 36 52.0 51.4

[0109] Specifically, according to the comparison results of Table 2.1, Table 2.3 and Table 2.4, the following conclusions are drawn: (1) By increasing the conditions such as temperature, relative humidity, time, etc. of each reaction, the physical and chemical properties of the microbial ecological bacterial fertilizer, the sugar content of silage corn and soil properties, and the growth indexes of silage corn all increase to a certain extent.

[0110] So far, the technical solution of the present invention has been described in conjunction with the preferred embodiments shown in the accompanying drawings. However, it is easily understood by those skilled in the art that the protection scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will fall within the protection scope of the present invention.

[0111] The above are only the preferred embodiments of the present invention and are not used to limit the present invention. For those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent substitution, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A preparation method of a microbial ecological bacterial fertilizer for increasing the sugar content of silage corn, characterized in that, Including: Inoculate Streptomyces pactum and Streptomyces rochei into two activation media for cultivation respectively to obtain a first strain spore suspension and a second strain spore suspension; Inoculate the first strain spore suspension and the second strain spore suspension into a solid substrate for fermentation respectively to obtain a first fermentation broth and a second fermentation broth; Filter and remove impurities from the first fermentation broth and the second fermentation broth, and then mix them to obtain an actinomycetes agent; Mix the decomposed sheep manure and sucrose residue with a preset mass percentage evenly to obtain a compost substrate, and carry out compost fermentation on the compost substrate for a preset time to obtain organic fertilizer; Cut the cleaned brown algae into pieces and add them to a stirring container together with deionized water, and obtain a seaweed extract in the stirring container under preset stirring conditions; Filter and remove impurities from the seaweed extract, and then transport it to a concentration device to obtain a concentrated seaweed extract under preset concentration conditions; Add the actinomycetes agent, corn flour and soybean amino acid powder to a double-shaft mixer, and obtain a premixed material under preset premixing conditions. Add the organic fertilizer, chemical fertilizer and trace elements to the premixed material, and the double-shaft mixer obtains a main mixed material under preset main mixing conditions; Granulate and dry the main mixed material to obtain dry granules, and spray a polyglutamic acid film with a concentration of 0.5% on the surface of the dry granules to obtain a microbial ecological bacterial fertilizer.

2. The preparation method of the microbial ecological bacterial fertilizer for increasing the sugar content of silage corn according to claim 1, characterized in that, The weight ratio of the activation medium is 4% glucose, 4% yeast extract powder, 10% malt extract powder, 6% peptone, 14% agar, 62% distilled water. The pH of the activation medium is 7.2, the temperature is 25°C, the culture time is 48 h, and the shaker speed is 180 rpm.

3. The preparation method of the microbial ecological bacterial fertilizer for increasing the sugar content of silage corn according to claim 1, characterized in that, The mass ratio of the solid substrate is wheat bran:diatomite = 3:1, and the fermentation conditions are set as the temperature of 28°C - 30°C and the relative humidity of 60°C - 70°C.

4. The preparation method of the microbial ecological bacterial fertilizer for increasing the sugar content of silage corn according to claim 3, characterized in that, The mixing conditions for mixing the first fermentation broth and the second fermentation broth are that the temperature range is set at 25°C - 30°C, the pH is 6.5 - 7.5, the mixing ratio is 7:3 by volume, the stirring speed is 100 rpm - 150 rpm, the mixing time is 2 h - 4 h, and sterile air with a saturation of 20% - 40% is introduced.

5. The preparation method of the microbial ecological bacterial fertilizer for increasing the sugar content of silage corn according to claim 3, characterized in that, The preset mass percentage of the sucrose residue is 2%, the mixing ratio of the decomposed sheep manure and the sucrose residue is 4:1 by mass, the preset time is set at 30 - 35 days, and the pH of the organic fertilizer is 6.8 - 7.

6.

6. The preparation method of the microbial ecological bacterial fertilizer for increasing the sugar content of silage corn according to claim 1, characterized in that, The size of the cut brown algae is a square block with a side length of 0.5 cm. The mixing ratio of the brown algae and the deionized water is 1:10 by mass. The preset stirring conditions are that the stirring speed is set at 200 rpm - 250 rpm, the temperature is set at 50°C - 60°C, and the time is set at 1.5 h - 2 h.

7. The preparation method of the microbial ecological bacterial fertilizer for increasing the sugar content of silage corn according to claim 1, characterized in that, The preset concentration conditions are that the concentration temperature is set at 40°C - 50°C, the vacuum degree is set at 0.08 MPa - 0.1 MPa, and the concentration time is set at 1 h - 1.5 h.

8. The preparation method of the microbial ecological bacterial fertilizer for increasing the sugar content of silage corn according to claim 1, characterized in that, The mixing ratio of the actinomycete inoculant, the corn flour, and the soybean amino acid powder is 1:3:6 by weight. The preset premixing conditions are that the premixing speed is set to 20 rpm to 30 rpm, the temperature is set to 25 °C to 30 °C, and the time is set to 10 min to 15 min.

9. The preparation method of the microbial ecological bacterial fertilizer for increasing the sugar content of silage corn according to claim 8, characterized in that, The mixing ratio of the premixed material, the organic fertilizer, the chemical fertilizer, and the trace elements is 4:3:2:1 by weight. The preset main mixing conditions are that the main mixing speed is set to 40 rpm to 50 rpm, the temperature is set to 25 °C to 30 °C, and the time is set to 20 min to 30 min.

10. A microbial ecological bacterial fertilizer for increasing the sugar content of silage corn, prepared by the method according to any one of claims 1-9, characterized in that, The microbial ecological bacterial fertilizer is composed of the following raw materials: Streptomyces pactum, Streptomyces rochei, well-rotted sheep manure, sugar cane residue, concentrated seaweed extract, corn flour, soybean amino acid powder, organic fertilizer, chemical fertilizer, trace elements, and polyglutamic acid film.

Images