Planting method for promoting early survival and growth of Panax notoginseng

By treating seeds with gibberellins and cymene and using Bacillus thuringiensis nutrients, combined with shade management and fertilization, the problems of low early survival and growth efficiency of Panax notoginseng were solved, early uniform seedling emergence and improved root development were achieved, and the rate of strong seedlings was increased.

CN120283620BActive Publication Date: 2025-09-30INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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Patent Information

Application Number
CN202510779013.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-06-12
Publication Date
2025-09-30
Estimated Expiration
2045-06-12

AI Technical Summary

Technical Problem

In the existing Panax notoginseng planting method, the early survival and growth efficiency of Panax notoginseng seedlings are low, resulting in a low seedling rate and increasing planting costs and time.

Method used

The seeds were treated with gibberellin solution and cymene in a closed fumigation process, combined with Bacillus thuringiensis fermentation nutrient agent, shade management and timely fertilization to promote the early emergence and root development of Panax notoginseng seedlings.

Benefits of technology

It significantly improved the uniformity of early emergence and root development of Panax notoginseng seedlings, increased the rate of strong seedlings, and reduced planting costs and time.

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Abstract

The present invention provides a planting method for promoting the early survival and growth of Panax notoginseng, and relates to the technical field of Panax notoginseng planting. The planting method mainly comprises the following steps: disinfecting and hormone-treating the Panax notoginseng seeds in the early stage, fumigating them with a certain concentration of cymene, and then watering them with a sterilized solution of pine needles and corn fermented with Bacillus thuringiensis after sowing. The present invention overcomes the shortcomings of the existing technology, can effectively improve the early emergence efficiency of Panax notoginseng, ensure uniform emergence of seedlings, promote root growth of Panax notoginseng seedlings, increase the vigorous seedling rate of Panax notoginseng seedlings, and comprehensively improve the economic benefits of Panax notoginseng planting.
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Description

Technical Field

[0001] The present invention relates to the technical field of Panax notoginseng planting, and in particular to a planting method for promoting early survival and growth of Panax notoginseng. Background Art

[0002] Panax notoginseng, also known as "Golden Root", is a perennial herbaceous plant in the Araliaceae family and genus Panax ginseng. It is a traditional, valuable, and widely cultivated Chinese medicinal herb that was first cultivated in my country. It is primarily grown in Wenshan, Yunnan, and has recently been cultivated sporadically in Guangdong, Sichuan, Guizhou, and Hunan. Its rhizome is used as a medicinal herb, known for its efficacy in reducing swelling, alleviating pain, dispersing blood stasis, and stopping bleeding. It is primarily used to treat cardiovascular and cerebrovascular diseases, hematologic disorders, and central nervous system disorders. Panax notoginseng typically waits three years after planting before being harvested, making continuous cropping a serious problem.

[0003] The main method of planting Panax notoginseng is sowing. Before sowing, it is usually necessary to pre-treat the seeds to break the dormancy period to promote the subsequent germination of seeds. Panax notoginseng can generally be transplanted after growing for 1 year or more after sowing. The survival rate of transplantation is closely related to the strength of the Panax notoginseng seedlings. Panax notoginseng seedlings with developed root systems and thick stems have a higher survival rate when transplanted. Therefore, in order to improve the strength rate of Panax notoginseng, it is often necessary to extend the seedling raising time of Panax notoginseng, which increases the cost of artificial planting of Panax notoginseng. Therefore, at this stage, improving the early growth of Panax notoginseng seedlings and promoting the strength of Panax notoginseng seedlings are important directions of artificial planting. Summary of the Invention

[0004] In response to the shortcomings of the existing technology, the present invention provides a planting method for promoting the early survival and growth of Panax notoginseng, which can effectively improve the early emergence efficiency of Panax notoginseng and ensure uniform emergence of seedlings, while promoting the root growth of Panax notoginseng seedlings, increasing the seedling rate of Panax notoginseng seedlings, and comprehensively improving the economic benefits of Panax notoginseng planting.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0006] A planting method for promoting early survival and growth of Panax notoginseng, the planting method comprising the following steps:

[0007] S1. Seed pretreatment: After the seeds are disinfected, they are soaked in a gibberellin solution to break dormancy, and then placed in a concentration of 0.2-0.5g / m 3 Treat the cymene in a closed environment for 1-2 hours to obtain pretreated seeds for later use;

[0008] S2. Preparation of nutrient agent: Mix pine needles and corn, add 3-5 times the weight of water, grind, inoculate with Bacillus thuringiensis, ferment for 2-4 days, sterilize, squeeze and filter to obtain nutrient agent for later use;

[0009] S3. Sowing: Sow the pretreated seeds in the prepared land, cover with a thin layer of soil, then water thoroughly with 100-150 times diluted nutrient solution, and cover with mulch or a layer of straw;

[0010] S4. Seedling management: After the emergence rate exceeds 20%, water the plants again with 100-150 times diluted nutrient solution;

[0011] S5. Fertilizer management: Add compound fertilizer once 4-5 months and 7-8 months after sowing.

[0012] Preferably, the seeds are disinfected in step S1 by soaking them in a 1000-fold diluted 50% carbendazim solution for 10-15 minutes and then taking them out. The seeds are soaked in a gibberellin solution with a concentration of 100-200 mg / L for 8-12 hours.

[0013] Preferably, in step S2, the mass ratio of pine needles to corn is 1-3:1-3, and the amount of inoculated Bacillus thuringiensis is 2%-3% of the total mass of pine needles and corn.

[0014] Preferably, the bacterial content of Bacillus thuringiensis in step S2 is 10 8 -10 10 pcs / g.

[0015] Preferably, the sterilization method in step S2 is to heat the mixture to 119-121° C. at a pressure of 0.4-0.6 MPa for 20-30 minutes.

[0016] Preferably, the sowing time in step S3 is from November of the current year to January of the following year, and a shade shed is built in time after sowing to control the shading rate at 30%-40% from July to February and at 60%-70% from March to June.

[0017] Preferably, the land prepared in advance in step S3 is first plowed and weeded, then quicklime is applied for disinfection, and then well-rotted manure and superphosphate are used as base fertilizer, and then the land is raked and leveled to form a 1.2-1.5m wide and 20-30cm high bed, with a furrow width of 30-40cm, and the spacing of the seeds sown is 5-7cm.

[0018] Preferably, the amount of the nutrient agent diluted 100-150 times for irrigation in step S4 is 200-300 kg / mu.

[0019] Preferably, the compound fertilizer in step S5 is 10-10-30 compound water-soluble fertilizer, and the single dosage is 3-5 kg / mu.

[0020] The present invention provides a planting method for promoting early survival and growth of Panax notoginseng, which has the following advantages over the prior art:

[0021] The present invention sterilizes the Panax notoginseng seeds and then uses cymene to fumigate in a closed space. Unlike the existing research in which cymene is used as a disinfectant and antibacterial component to act directly on the soil, the air fumigation method can promote the subsequent uniformity of seed germination to a certain extent, and promote the development of the root system of the Panax notoginseng seedlings after early germination to a certain extent. At the same time, the nutrient agent prepared by fermenting and sterilizing Bacillus thuringiensis is subsequently used for watering, which further promotes the development of the roots of the Panax notoginseng seedlings, and simultaneously promotes the expansion of the roots and the growth of stems and leaves, thereby comprehensively improving the growth efficiency of the Panax notoginseng seedlings and increasing the seedling growth rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a comparison chart of the rooting conditions of Panax notoginseng seedlings in each group on March 30, 2024 in the embodiment of the present invention;

[0023] Figure 2 This is a comparison chart of the growth conditions of experimental group 6, experimental group 7, experimental group 4 and experimental group 8 when collected on November 21, 2024 in an embodiment of the present invention. DETAILED DESCRIPTION

[0024] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention are clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0025] The Bacillus thuringiensis used in the following examples has a deposit number of CGMCC No. 20588 and an activity of 10 9 pcs / g.

[0026] Example 1

[0027] Panax notoginseng sowing experiment:

[0028] 1. Selection and preliminary processing of seed raw materials:

[0029] Select mature seeds of three-year-old Panax notoginseng, peel them, wash them, and then soak them in a 1000-fold diluted 50% carbendazim solution for 10-15 minutes. Then take them out and soak them in a 150 mg / L gibberellin solution for 10 hours to sterilize the seeds.

[0030] The sterilized seeds were placed in a closed environment with different concentrations of cymene and fumigated for 90 minutes with reference to Table 1 below, to obtain different pretreated seeds for later use;

[0031] Table 1

[0032] 2. Preparation of nutrients:

[0033] Nutrient 1: Pine needles and corn were mixed in equal proportions, the mixture was ground into a mixture of 4 times its mass of water, and then inoculated with 2% of the total mass of the mixture, and fermented for 3 days. The mixture was then heated to 120°C and sterilized at 0.5 MPa for 25 minutes. The filtrate was squeezed and filtered to obtain Nutrient 1.

[0034] Nutrient 2: Pine needles and corn were mixed in equal proportions, the mixture was pulverized with 4 times the mass of water, and then inoculated with 2% of the total mass of the mixture, and fermented for 3 days. The mixture was then squeezed and filtered to obtain the filtrate as Nutrient 2.

[0035] 3. Treatment of the sowing experimental site:

[0036] A Panax notoginseng planting experiment was carried out in a designated area at the Panax notoginseng planting base in Yanshan County, Yunnan Province. Panax notoginseng had not been planted in this area within three years. Before sowing, the land was plowed and weeded, and then 150 kg of quicklime was spread per mu for disinfection. After an interval of 10 days, 1800 kg of decomposed manure and 50 kg of calcium phosphate were applied per mu as base fertilizer. The land was then raked and leveled to make ridges with a width of 1.2-1.5 m and a height of 20-30 cm, and the ridge furrows were 30-40 cm wide. A shade net was then set up on the land to control the shading rate at 30%-40% from July to February and at 60%-70% from March to June. The planting fields were well treated.

[0037] 4. Seeding experiment:

[0038] On December 21, 2023, the above-mentioned different pretreated seeds were sown in the above-mentioned different planting fields at an interval of 5-7 cm. After sowing, the nutrient agent diluted 120 times was used to water the fields thoroughly and then covered with a layer of straw. On February 1, 2024, the film was removed and the 120-fold diluted nutrient agent was used again at a dosage of 250 kg / mu. On April 26, 2024, 10-10-30 (10% N, 10% P2O5, 30% K2O) compound water-soluble fertilizer was used for supplementary fertilizer (5 kg / mu). Then, on July 22, 2024, 10-10-30 compound water-soluble fertilizer was used for supplementary fertilizer (3 kg / mu). The supplementary fertilizer method was to dissolve the compound water-soluble fertilizer in water and then irrigate.

[0039] Multiple experiments were set up according to the pre-treated seeds and nutrients in Table 2 below:

[0040] Table 2

[0041] 5. Observation and recording of experimental results:

[0042] 5.1. Observe and record the emergence rate of each group on February 1, February 15, and March 5, 2025. The specific results are shown in Table 3 below:

[0043] Table 3

[0044] As shown in Table 3 above, the germination rates of experimental groups 4-6 and experimental group 8 on February 1 were all above 35%. Therefore, fumigating seeds with a certain concentration of cymene can effectively promote early germination of seeds. At higher concentrations, the efficiency of promoting germination is significantly reduced. That is, by comparing experimental groups 3, 4, 8, and 7, the optimal fumigation concentration is 0.2-0.5 g / m 3 In addition, the use of different nutrients also has a certain effect on the emergence rate. Among them, nutrient 1 has the best effect in promoting emergence compared with nutrient 2 and clean water.

[0045] 5.2. On March 30, 2024, 10 plants with a seedling height of 4.2-4.6 cm were randomly collected from each experimental group for root growth observation. The number of lateral roots and the average root length of each group were recorded. The specific results are shown in Tables 4 and Figure 1 As shown:

[0046] Table 4

[0047] From the above table and Figure 1 It can be seen that the root growth of experimental groups 4, 7 and 8 is the best.

[0048] 5.3. On November 21, 2024, 5 Panax notoginseng seedlings were randomly collected from each group. The seedling height, stem diameter, and taproot diameter of each group of Panax notoginseng seedlings were observed and recorded. The specific results are shown in Table 5 below:

[0049] Table 5

[0050] It can be seen from the above table that the treatments with different nutrients have the greatest impact on the seedling height, stem diameter and main root diameter of Panax notoginseng one-year seedlings, among which the effect of nutrient 1 is the best.

[0051] According to the above experiments, combined with Figure 2 The Panax notoginseng seedlings of experimental group 6, experimental group 7, experimental group 4 and experimental group 8 were compared respectively. Among them, the main root enlargement effect of Panax notoginseng seedlings in experimental group 4 and experimental group 8 was the best, and the stems were stronger.

[0052] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A planting method for promoting early survival and root growth of Panax notoginseng, characterized in that: The planting method comprises the following steps: S1. Seed pretreatment: After the seeds are disinfected, they are soaked in a gibberellin solution to break dormancy, and then placed in a concentration of 0.2-0.5g / m 3 Fumigation treatment is performed on cymene in a closed environment for 1-2 hours to obtain pre-treated seeds for later use; S2. Preparation of nutrient agent: Mix pine needles and corn, add 3-5 times the weight of water, grind, inoculate with Bacillus thuringiensis, and ferment for 2-4 days. Sterilize, squeeze, and filter to obtain the nutrient agent for later use. The mass ratio of pine needles to corn is 1-3:1-3, and the amount of Bacillus thuringiensis inoculated is 2%-3% of the total mass of pine needles and corn. S3. Sowing: Sow the pretreated seeds in the prepared land, cover with a thin layer of soil, then water thoroughly with 100-150 times diluted nutrient solution, and cover with mulch or a layer of straw; S4. Seedling management: After the emergence rate exceeds 20%, water the plants again with 100-150 times diluted nutrient solution; S5. Fertilizer management: Add compound fertilizer once 4-5 months and 7-8 months after sowing.

2. The planting method according to claim 1, characterized in that: In step S1, the seeds are disinfected by soaking them in a 1000-fold diluted 50% carbendazim solution for 10-15 minutes and then taking them out. The seeds are soaked in a gibberellin solution with a concentration of 100-200 mg / L for 8-12 hours.

3. The planting method according to claim 1, characterized in that: The bacterial content of Bacillus thuringiensis in step S2 is 10 8 -10 10 pcs / g.

4. The planting method according to claim 1, characterized in that: The sterilization method in step S2 is to heat the mixture to 119-121° C. under a pressure of 0.4-0.6 MPa for 20-30 minutes.

5. The planting method according to claim 1, characterized in that: The sowing time in step S3 is from November of the current year to January of the following year, and a shade shed is built in time after sowing to control the shading rate to be 30%-40% from July to February and 60%-70% from March to June.

6. The planting method according to claim 1, characterized in that: The land prepared in advance in step S3 is first plowed and weeded, then quicklime is applied for disinfection, and then well-rotted manure and superphosphate are used as base fertilizer. The land is then raked and leveled to form a 1.2-1.5m wide and 20-30cm high bed, with a furrow width of 30-40cm and a seed sowing interval of 5-7cm.

7. The planting method according to claim 1, characterized in that: The amount of the nutrient agent diluted 100-150 times for irrigation in step S4 is 200-300 kg / mu.

8. The planting method according to claim 1, characterized in that: The compound fertilizer in step S5 is 10-10-30 compound water-soluble fertilizer, and the single dosage is 3-5 kg / mu.