Intestinal antioxidant peptide and zinc glycan complex as well as preparation method and application thereof

Abstract

The invention discloses an intestinal antioxidant peptide and zinc glycan complex as well as a preparation method and application thereof, and belongs to the technical field of functional foods and medicines. The complex is insoluble in water, has better antioxidant activity, can play an antioxidant role in intestinal tracts and can promote intestinal health. According to the intestinal antioxidant peptide and zinc glycan complex, antioxidant polypeptides such as polyglutamic acid, polyaspartic acid, whey protein and glutathione and zinc with an antioxidant effect are creatively selected as antioxidant functional components, and glucomannan molecules are used as a three-dimensional network wall material, so that the antioxidant functional components are successfully fixed; digestion and absorption are prevented, and an anti-oxidation effect can be achieved in the enteric cavity. The preparation process solves a plurality of technical problems of molecular cross-linking entanglement and polypeptide solidification.

Description

Technical Field

[0001] The present invention belongs to the technical fields of functional foods and medicine, and particularly relates to an intestinal antioxidant peptide zinc glycan complex, a preparation method thereof, and an application thereof. Background Art

[0002] Intestinal health mainly considers three aspects: intestinal flora, intestinal mucosal barrier, and free radicals. The intestinal flora refers to the situation where beneficial bacteria, neutral bacteria, and harmful bacteria in the intestine form a tripartite balance, with one rising and the other falling. When the intestinal flora is disordered, clinical symptoms such as constipation, diarrhea, intestinal ulcer, and intestinal perforation will occur. The intestinal mucosal barrier refers to the structure and function of the intestine that can prevent harmful substances in the intestine, such as bacteria and toxins, from passing through the intestinal mucosa and entering other tissues, organs, and blood circulation in the human body. Once the intestinal mucosal barrier is damaged, intestinal peristalsis slows down, the absorption of nutrients is insufficient, the metabolism of residue waste is incomplete, which will lead to malnutrition, toxin accumulation, intestinal ulcer, and even high-risk lesions such as perforation. The intestine is a place where free radicals are extremely likely to accumulate. It has been found that staying up late causes a large amount of free radicals to accumulate in the intestine. The intestine itself is prone to form various free radicals. Under the catalysis of staying up late, the free radicals in the intestine multiply, which is extremely likely to accelerate intestinal aging, damage the barrier integrity, lead to flora disorder, and then cause a series of major diseases. Therefore, it is crucial to remove free radicals in the intestine.

[0003] The part of the intestine where the most free radicals accumulate is the colon. Almost all the antioxidant components contained in the daily intake of food and oral antioxidant drugs are absorbed in the small intestine and rarely reach the colon to play an antioxidant role in the colon. It has been found that some antioxidant peptides have the effects of scavenging free radicals, antioxidant, antibacterial, and inhibiting lipid peroxidation. It has been shown that polyglutamic acid, polyaspartic acid, whey protein, and glutathione have certain antioxidant activities.

[0004] In addition, an indispensable metal element in the antioxidant effect is zinc. Zinc is an essential trace element for maintaining normal growth, cognitive behavior, wound healing, taste, and immune regulation of the body and for more than 200 metal enzymes to function. In terms of antioxidant, it has been found that zinc weakens the ability of transition metals to provide free electrons by competing for binding sites with transition metals, preventing the generation of hydroxyl radicals by transition metals. On the other hand, zinc can prevent the oxidation of sulfhydryl groups on proteins. Zinc realizes its antioxidant effect by regulating the metabolism of metallothionein. The lack of metallothionein can lead to damage to the antioxidant defense system of body cells and enhance the sensitivity of cells to oxidative stress. Although the antioxidant mechanism of zinc is not fully understood at present, it has been clear that zinc deficiency has a significant impact on the antioxidant capacity of body cells. In vivo and in vitro experimental results have shown that when zinc is deficient, the number of free radicals increases and the damage increases. For example, compared with mice in the zinc-sufficient group, the probability of carbon tetrachloride poisoning and lipid peroxidation is higher in the zinc-deficient group of mice.

[0005] Zinc is a strong electron acceptor and has a strong binding force with thiol and amine electron donors. Zinc also has a rapid ligand exchange effect, which is particularly important for the catalytic action of metalloenzymes. Zinc can provide a highly localized charge center and thus can become a very good attacking group, especially at those sites that are restricted and weakly bound to the substrate. It has been found that the antioxidant effect of zinc depends on the zinc concentration in the microenvironment, and only at high concentrations does it have a high antioxidant effect. In addition, a high concentration of zinc in the microenvironment also has a better ability to avoid heavy metals, displace and promote the excretion of heavy metals such as arsenic, lead, and mercury from the body.

[0006] Existing products include oral antioxidant peptides. However, oral antioxidant peptides will be hydrolyzed by digestive enzymes into amino acids and absorbed in the small intestine. Zinc in zinc supplementation preparations is absorbed in the small intestine after oral administration. Although a part is excreted into the intestine through the enterohepatic circulation, the zinc concentration in the colon is relatively low and cannot reach the threshold concentration for effective antioxidant action. Therefore, so far, there is still no material on the market that can exert antioxidant action in the intestinal lumen. Summary of the Invention

[0007] Aiming at the current lack of antioxidant materials in the intestinal lumen, the first object of the present invention is to provide a preparation method of an intestinal antioxidant peptide-zinc glycan complex. This preparation method is easy to operate, has high production efficiency, and is suitable for industrial production.

[0008] The second object of the present invention is to provide an antioxidant peptide-zinc glycan complex. This complex is insoluble in water, has good antioxidant activity, can exert antioxidant action in the intestine, and promotes intestinal health.

[0009] The third object of the present invention is to provide an application of an intestinal antioxidant peptide-zinc glycan complex, which can be used as an intestinal antioxidant to promote intestinal health and can be used as a basic raw material or finished product for preparing functional foods and pharmaceutical products for the prevention and treatment of various diseases.

[0010] The present invention is achieved through the following technical solutions:

[0011] A preparation method of an intestinal antioxidant peptide-zinc glycan complex, comprising the following steps:

[0012] S1. Select 1-3 parts of antioxidant peptide, 0.5-2 parts of glycerol, and 0.1-0.5 part of glucomannan, mix and dissolve them in water, stir and keep warm at 70°C - 95°C for more than 30 minutes, add 0.1-0.3 part of basic amino acid, continue to stir and keep warm at 70°C - 95°C for more than 30 minutes to obtain a mixed solution A;

[0013] S2. Take another 3-5 parts of glucomannan and mix it with a basic compound, and then add it to solution A under rapid stirring, and keep warm at 85°C - 95°C for more than 60 minutes;

[0014] After the glucomannan is mixed with the alkaline compound and then added to Liquid A, the total amount is 100 parts.

[0015] S3. Then, it is prepared through low-temperature freezing, mechanical crushing, rinsing and dehydration, being placed in a zinc compound solution with a mass concentration of not less than 3% for stirring and heat preservation treatment, filtration, and drying and pulverization.

[0016] In the preparation of this intestinal antioxidant peptide zinc glucomannan complex, the common raw materials used, the production and preparation method is easy to operate, and the production efficiency is high, which is suitable for industrial scale-up.

[0017] Preferably, the mass fraction of the zinc compound solution is 10 - 15 parts.

[0018] Preferably, the zinc compound solution is a solution of one or a mixture of zinc chloride solution, zinc gluconate solution, zinc glycyrrhizinate solution, zinc acetate solution, zinc citrate solution, zinc lactate solution.

[0019] Preferably, the antioxidant peptide is one or a combination of polyglutamic acid, polyaspartic acid, whey protein, glutathione.

[0020] Preferably, the basic amino acid is one or a combination of arginine, lysine, histidine.

[0021] Preferably, the alkaline compound is a monovalent metal alkaline compound.

[0022] Preferably, the alkaline compound is one or a combination of sodium carbonate, sodium bicarbonate, sodium hydroxide, potassium hydroxide.

[0023] Preferably, in S3, it is placed in a zinc compound solution with a mass concentration of not less than 3% for stirring and heat preservation at 85°C - 95°C for not less than 60 min.

[0024] An intestinal antioxidant peptide zinc glucomannan complex, characterized in that it is obtained by the preparation method of the intestinal antioxidant peptide zinc glucomannan complex described above.

[0025] The above-mentioned intestinal antioxidant peptide-zinc polysaccharide complex selects polypeptide molecules with antioxidant effects such as polyglutamic acid, polyaspartic acid, whey protein, glutathione, etc. and zinc with antioxidant effects for combination to ensure antioxidant activity. To solve the technical problem of being digested and absorbed by intestinal proteases, at the same time, glucomannan is creatively used as an antioxidant component protective wall material, and through crosslinking into a three-dimensional network, the antioxidant components are wrapped and solidified to resist enzymatic hydrolysis. However, to fix the polypeptide molecular chain and zinc in the glucomannan molecular network, the following key technical problems and process contradictions are faced: First, the spatial structure of the antioxidant polypeptide molecule, the negative charge of the polypeptide molecular chain, and the positive charge of the zinc ion will all seriously affect the molecular crosslinking of glucomannan. Second, the antioxidant polypeptide molecular chain lacks branches, and how to be fixed in the polysaccharide network. Third, the antioxidant polypeptide is not damaged under the process conditions. Fourth, how to improve the zinc ion complexation efficiency.

[0026] To solve the above technical problems, the antioxidant polypeptide molecule and the glucomannan molecule are creatively pretreated at high temperature. The purpose of using high temperature is to stretch the polypeptide molecular chain, avoid the influence of the spatial structure on the entanglement of the glucomannan molecule, and at the same time fully expose the peptide chain groups. The acetyl group of the glucomannan molecule is used to combine with the amino group on the stretched polypeptide molecular chain, and glycerol is used to prevent the polypeptide molecular chain from being broken in the high and low temperature environments. Then, basic amino acids are creatively selected to neutralize and shield the negative charge of the polypeptide, and the zinc ion complexation process and the molecular crosslinking process are separated to avoid the inhibition of molecular crosslinking by charge. Finally, glucomannan with a high concentration (the raw material concentration is 3.5-5.1% glucomannan) is used to reduce the intermolecular distance, and glycerol is used to promote the exposure of the flexible region of the molecular chain and promote molecular sliding entanglement. To improve the zinc ion complexation efficiency, the peptide molecular chain is stretched at high temperature to fully expose the zinc ion complexation site, and a higher zinc concentration is used for complexation. In addition, a monovalent metal base is preferably used as a crosslinking aid to avoid selecting a divalent or trivalent metal base, which affects the complexation of the zinc ion in the subsequent process.

[0027] An application of an intestinal antioxidant peptide-zinc polysaccharide complex, which is the application of the intestinal antioxidant peptide-zinc polysaccharide complex in the preparation of intestinal antioxidants, functional foods and pharmaceutical products for the prevention and treatment of various diseases.

[0028] Compared with the prior art, the present invention has at least the following technical effects:

[0029] (1) The present invention provides an intestinal antioxidant peptide-zinc polysaccharide complex, which is insoluble in water, has good antioxidant activity, can play an antioxidant role in the intestine, and promote intestinal health. The intestinal antioxidant peptide-zinc polysaccharide complex creatively selects antioxidant polypeptides such as polyglutamic acid, polyaspartic acid, whey protein, glutathione, and zinc with antioxidant effects as antioxidant functional components, and uses glucomannan molecules as the three-dimensional network wall material to successfully immobilize the antioxidant functional components, prevent digestion and absorption, and can play the antioxidant role in the intestinal lumen.

[0030] (2) Ingeniously design the preparation process to solve multiple technical problems such as molecular cross-linking entanglement and polypeptide solidification. Creatively use high-temperature conditions to stretch polypeptide molecules, select an appropriate proportion of basic amino acids to neutralize the charges of antioxidant polypeptides, separate the zinc ion complexation process from the molecular cross-linking process, select an appropriate concentration of glycerol to protect polypeptide molecules, promote the sliding entanglement of glucomannan molecules, and preferably use monovalent metal bases as cross-linking agents to further improve the complexation efficiency of zinc ions.

[0031] (3) The common raw materials used are easy to operate in the production preparation method, with high production efficiency and suitable for industrial scale-up.

[0032] (4) The prepared antioxidant peptide-zinc complex is insoluble in water, can play an antioxidant role in the intestine, promote intestinal health, and can be used as a functional food and pharmaceutical product for the prevention and treatment of various diseases. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 The product prepared in Example 1. DETAILED DESCRIPTION OF THE INVENTION

[0034] The following will describe the implementation plan of the present invention in detail in combination with examples. However, those skilled in the art will understand that the following examples are only used to illustrate the present invention and should not be regarded as limiting the scope of the present invention. The specific conditions not specified in the examples are carried out according to conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments not specified in the manufacturer are all conventional products that can be obtained through commercial purchase.

[0035] Example 1:

[0036] A preparation method of an intestinal antioxidant peptide-zinc polysaccharide complex, comprising the following preparation steps:

[0037] S1. Dissolve 2 kg of glutathione, 1 kg of glycerol, and 0.3 kg of glucomannan in 92.2 kg of water, stir and keep warm at 85 °C for 50 min, add 0.2 kg of arginine, continue to stir and keep warm at 85 °C for 50 min to obtain a mixed solution A;

[0038] S2. Take 4 kg of glucomannan and 0.3 kg of sodium bicarbonate, mix them evenly, and add them to liquid A under rapid stirring, then keep it at 90 °C for 80 min;

[0039] S3. Prepare it by low-temperature freezing, mechanical crushing, rinsing and dehydration, placing it in a zinc chloride solution with a mass concentration of 6% and stirring, keeping it at 90 °C for 90 min, filtering, and drying and pulverizing.

[0040] The specific product is as Figure 1 shown.

[0041] Example 2:

[0042] A preparation method of an intestinal antioxidant peptide zinc polysaccharide complex, comprising the following preparation steps:

[0043] S1. Dissolve 0.4 kg of polyglutamic acid, 0.6 kg of polyaspartic acid, 0.5 kg of glycerol, and 0.1 kg of glucomannan in 95.2 kg of water, stir and keep it at 70 °C for 30 min, add 0.1 kg of lysine, and continue to stir and keep it at 70 °C for 30 min to obtain mixed liquid A;

[0044] S2. Take 3 kg of glucomannan and 0.1 kg of sodium carbonate, mix them evenly, and add them to liquid A under rapid stirring, then keep it at 85 °C for 60 min;

[0045] S3. Prepare it by low-temperature freezing, mechanical crushing, rinsing and dehydration, placing it in a solution containing 1% zinc gluconate + 2% zinc glycyrrhizinate by mass concentration, stirring, keeping it at 85 °C for 60 min, filtering, and drying and pulverizing.

[0046] Example 3:

[0047] A preparation method of an intestinal antioxidant peptide zinc polysaccharide complex, comprising the following preparation steps:

[0048] S1. Dissolve 3 kg of serum protein, 2 kg of glycerol, and 0.5 kg of glucomannan in 88.7 kg of water, stir and keep it at 95 °C for 30 min, add 0.1 kg of arginine and 0.2 kg of histidine, and continue to stir and keep it at 70 °C for 50 min to obtain mixed liquid A;

[0049] S2. Take 5 kg of glucomannan, 0.4 of sodium hydroxide, and 0.1 kg of potassium hydroxide, mix them evenly, and add them to liquid A under rapid stirring, then keep it at 85 °C for 90 min;

[0050] S3. Prepare it by low-temperature freezing, mechanical crushing, rinsing and dehydration, placing it in a solution containing 2% zinc glycyrrhizinate + 2% zinc acetate + 2% zinc citrate + 4% zinc lactate by mass concentration, stirring, keeping it at 95 °C for 100 min, filtering, and drying and pulverizing.

[0051] Example 4:

[0052] A preparation method of an intestinal antioxidant peptide zinc glycan complex, comprising the following preparation steps:

[0053] S1. Dissolve 0.2 kg of polyglutamic acid, 0.4 kg of polyaspartic acid, 1.0 kg of whey protein, 0.7 kg of glutathione, 0.8 kg of glycerol, and 0.4 kg of glucomannan in 92.55 kg of water, stir and keep warm at 85 °C for 60 min, add 0.15 kg of histidine, continue to stir and keep warm at 70 °C for 40 min to obtain a mixed solution A;

[0054] S2. Take 3.5 kg of glucomannan, 0.1 kg of sodium carbonate, 0.2 kg of sodium bicarbonate, and 0.1 kg of sodium hydroxide, mix them evenly, and add them to solution A under rapid stirring, and keep warm at 85 °C for 60 min;

[0055] S3. Prepare by low-temperature freezing, mechanical crushing, rinsing and dehydration, placing it in a solution of 3% zinc chloride + 3% zinc gluconate + 2% zinc glycyrrhizinate by mass concentration, stirring and keeping warm at 85 °C for 60 min, filtering, and drying and pulverizing.

[0056] All the above mass percentages are mass concentrations.

[0057] Setting of comparative examples:

[0058] For further illustration and comparison, by changing the component or ratio or process flow parameters of the present invention, the products prepared all have defects, as shown in Table 1 below:

[0059] Table 1 List of preparation methods and results of comparative examples

[0060]

[0061]

[0062] Test example: Take the products of each example and comparative example, and conduct relevant performance measurements.

[0063] 1. Determination of antioxidant activity

[0064] Accurately pipette 3.90 mL of DPPH working solution, add 0.2 g of the sample of the example or comparative example respectively, incubate and develop color at 37 °C for 30 min, use absolute ethanol as the blank control, and measure the absorbance A of the supernatant of each sample at a wavelength of 517 nm; at the same time, use 0.2 g of granular activated carbon instead of the sample to conduct the measurement under the same conditions, and record the absorbance as A0. Measure in parallel 3 times, and calculate the free radical scavenging rate %.

[0065] Clearance rate = [(A0 - A) / A0] × 100%;

[0066] Where: A0 is the initial absorbance of DPPH; A is the absorbance of DPPH after adding the sample solution.

[0067] The results are shown in Table 2.

[0068] Table 2 Determination results of free radical scavenging rate (%)

[0069] sample clearance rate sample clearance rate sample clearance rate activated carbon particles 3.01±0.12 Example 4 64.15±8.11 Comparative Example 12 13.81±3.92 Example 1 79.20±8.92 Comparative Example 2 21.58±9.29 Comparative Example 17 15.42±5.21 Example 2 74.15±11.14 Comparative Example 3 11.31±5.45 Comparative Example 18 9.58±4.56 Example 3 70.15±12.75 Comparative Example 10 13.44±9.07 Comparative Example 19 8.07±4.13

[0070] In Examples 1 - 4, the scavenging rate of free radicals exceeded 50%, with relatively high antioxidant activity, while in the comparative examples and activated carbon particles, the antioxidant activity was relatively low.

[0071] 2. Antioxidant animal research

[0072] After 40 Kunming mice were adaptively fed in the mouse house for 7 days, they were randomly divided into 5 groups, with 8 mice in each group. The mice in the blank control group were intragastrically administered distilled water, and the mice in the other groups were intragastrically administered 0.4 g / kg of the samples of Examples 1 - 4. The mice were intragastrically administered once a day for 30 consecutive days. At the end of the experiment, all mice were fasted for 24 h, weighed, and sampled. During this period, the mice could drink water freely. After blood collection from the eye socket and decapitation, dissection was performed, the thymus and spleen were dissected, weighed, and the immune organ index was calculated.

[0073] Thymus index = thymus weight / body weight; spleen index = spleen weight / body weight; The activities of IgA, IgG, IgM, T - SOD, GSH - Px, MDA, and T - AOC in mouse serum were determined by colorimetry, and the contents of IL - 6, IL - 1β, and TNF - α in mouse serum were determined by enzyme - linked immunosorbent assay (ELISA); The operation was carried out according to the operation instructions of the relevant kits. The research results are shown in Table 3.

[0074] Table 3 Antioxidant animal serum indexes of the products of the examples

[0075] index normal group Example 1 Example 2 Example 3 Example 4 Thymus index (mg / g) 1.62±0.05 <![CDATA[1.85±0.09 * > <![CDATA[1.91±0.08 * > <![CDATA[1.72±0.08 * > <![CDATA[1.81±0.06 * > Spleen index (mg / g) 3.11±0.11 <![CDATA[3.58±0.21 * > <![CDATA[3.44±0.23 * > <![CDATA[3.71±0.25 * > <![CDATA[3.60±0.24 * > IgA (g / L) 2.72±0.22 <![CDATA[3.33±0.18 * > <![CDATA[3.51±0.14 * > <![CDATA[3.49±0.12 * > <![CDATA[3.49±0.17 * > IgG (g / L) 0.54±0.83 <![CDATA[0.58±0.03 * > <![CDATA[0.61±0.04 * > <![CDATA[0.61±0.05 * > <![CDATA[0.59±0.04 * > IgM (g / L) 1.19±0.08 <![CDATA[1.74±0.06 * > <![CDATA[1.85±0.05 * > <![CDATA[1.60±0.05 * > <![CDATA[1.86±0.06 * > T-SOD (U / mL) 48.8±3.08 <![CDATA[78.7±6.71 * > <![CDATA[82.2±8.40 * > <![CDATA[81.5±6.54 * > <![CDATA[80.0±6.45 * > GSH-Px (U / mL) 256.9±14.9 <![CDATA[316.4±15.2 * > <![CDATA[325.1±17.1 * > <![CDATA[321.3±15.0 * > <![CDATA[312.9±16.9 * > MDA (U / mL) 10.15±0.91 <![CDATA[14.13±1.07 * > <![CDATA[15.21±1.05 * > <![CDATA[15.35±1.08 * > <![CDATA[15.15±1.12 * > T-AOC (umol / L) 4.97±0.32 <![CDATA[3.17±0.20 * > <![CDATA[2.91±0.24 * > <![CDATA[2.67±0.21 * > <![CDATA[3.75±0.21 * > IL-6 (ng / L) 136.4±7.4 <![CDATA[105.9±9.2 * > <![CDATA[95.2±9.6 * > <![CDATA[99.0±9.4 * > <![CDATA[102.2±9.5 * > IL-1β (ng / L) 103.51±8.9 <![CDATA[80.60±7.6 * > <![CDATA[80.63±7.8 * > <![CDATA[79.86±7.3 * > <![CDATA[85.46±7.7 * > TNF-α (ng / L) 73.66±5.66 <![CDATA[51.9±3.62 * > <![CDATA[50.4±3.69 * > <![CDATA[52.5±3.41 * > <![CDATA[56.8±3.39 * >

[0076] Note: * Compared with the normal group, there was a statistical difference (P < 0.05)

[0077] As can be seen from Table 3, Examples 1 - 4 have good antioxidant activity.

[0078] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the protection scope of the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A preparation method of an intestinal antioxidant peptide zinc glycan complex, characterized in that, It includes the following steps: S1. Select 1 - 3 parts of antioxidant peptides, 0.5 - 2 parts of glycerol, and 0.1 - 0.5 part of glucomannan, mix and dissolve them in water, stir and keep warm at 70°C - 95°C for more than 30 minutes, add 0.1 - 0.3 part of basic amino acids, continue to stir and keep warm at 70°C - 95°C for more than 30 minutes to obtain mixture A; S2. Take another 3 - 5 parts of glucomannan, mix it with a basic compound, and then add it to solution A under rapid stirring, and keep warm at 85°C - 95°C for more than 60 minutes; After the glucomannan and the basic compound are mixed and added to solution A, the total amount is 100 parts; S3. Then it is prepared through low - temperature freezing, mechanical crushing, rinsing and dehydration, placing it in a zinc - containing compound solution with a mass concentration of not less than 3% for stirring and heat - preservation treatment, filtering, drying and pulverizing.

2. The preparation method of an intestinal antioxidant peptide-zinc glycan complex according to claim 1, characterized in that, The mass fraction of the zinc - containing compound solution is 10 - 15 parts.

3. The preparation method of an intestinal antioxidant peptide zinc glycan complex according to claim 2, characterized in that, The zinc - containing compound solution is one or a mixture of solutions of zinc chloride solution, zinc gluconate solution, zinc glycyrrhizinate solution, zinc acetate solution, zinc citrate solution, zinc lactate solution, etc.

4. The preparation method of an intestinal antioxidant peptide-zinc glycan complex according to claim 1, characterized in that, The antioxidant peptide is one or a combination of polyglutamic acid, polyaspartic acid, whey protein, glutathione, etc.

5. The preparation method of an intestinal antioxidant peptide-zinc glycan complex according to claim 1, wherein The basic amino acid is one or a combination of arginine, lysine, histidine, etc.

6. The preparation method of an intestinal antioxidant peptide-zinc glycan complex according to claim 1, characterized in that, The basic compound is a monovalent metal basic compound.

7. The preparation method of an intestinal antioxidant peptide-zinc glycan complex according to claim 6, wherein, The basic compound is one or a combination of sodium carbonate, sodium bicarbonate, sodium hydroxide, potassium hydroxide, etc.

8. The preparation method of an intestinal antioxidant peptide zinc glycan complex according to claim 1, characterized in that, In S3, place it in a zinc - containing compound solution with a mass concentration of not less than 3% for stirring and keep warm at 85°C - 95°C for not less than 60 minutes.

9. An intestinal antioxidant peptide zinc glycan complex, characterized in that, Obtained by the preparation method of the intestinal antioxidant peptide zinc glycan complex according to any one of claims 1 - 8.

10. Use of the intestinal antioxidant peptide zinc glycan complex according to claim 9, characterized in that, The application of the intestinal antioxidant peptide zinc glycan complex in the preparation of intestinal antioxidants, functional foods for preventing and treating various diseases, and pharmaceutical products.

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