Molecular breeding molecular marker kit

Abstract

The molecular breeding molecular marker kit comprises a kit body, a temperature control assembly is installed on the bottom end face of the kit body, a control and display system is installed on one side wall of the kit body, and a real-time monitoring system is installed at the position, located in the kit body, of the top end face of the temperature control assembly. Compared with the prior art, the molecular breeding molecular marker kit has the advantages that the multiple layers of storage bins are matched with the temperature control assemblies with various temperatures, so that different molecules can be placed in different storage bins according to proper use environments, and the molecular breeding molecular marker kit is convenient to use and high in practicability. The success probability and efficiency of a molecular breeding experiment are greatly improved; by adding the control and display system and the real-time monitoring system, reagents with corresponding numbers can be quickly found, and the state of molecules in each reagent tube can be observed and recorded in real time, so that the experiment efficiency is greatly improved, and the sustainable development of agriculture is promoted.

Description

Technical Field

[0001] The present invention belongs to the technical field of molecular breeding, and specifically relates to a molecular breeding molecular marker kit. Background Art

[0002] Molecular breeding applies molecular biology techniques to breeding and conducts breeding at the molecular level. It usually includes molecular marker-assisted breeding and genetic modification breeding. A molecular marker kit is required in the process of molecular breeding. This kit is used to place molecular breeding test tubes. During molecular breeding, different plant species, different growth stages, and different breeding objectives have different temperature requirements.

[0003] For different molecular breedings, storage environments at different temperatures are needed. The current molecular marker kits can only keep breeding molecules at one temperature, and some reagents may become ineffective due to improper storage; multiple molecules are placed in the molecular marker kit at the same time, and each time different reagents need to be labeled, and it takes a lot of time to find the corresponding reagents when taking them out; during molecular breeding, the status of each molecular reagent cannot be observed and recorded in real time in the molecular kit, greatly reducing the experimental efficiency.

[0004] Therefore, in view of the above technical problems, it is necessary to provide a molecular breeding molecular marker kit.

[0005] The information disclosed in this background art section is only intended to enhance the overall understanding of the present invention and should not be regarded as an admission or any form of suggestion that this information constitutes prior art already known to those of ordinary skill in the art. Summary of the Invention

[0006] The purpose of the present invention is to provide a molecular breeding molecular marker kit, which can solve the problems raised in the above background art.

[0007] To achieve the above purpose, the technical solution provided by a specific embodiment of the present invention is as follows: A molecular breeding molecular marker kit includes a box body. A temperature control component is installed on the bottom end face of the box body, a control and display system is installed on the top end face of the box body, a real-time monitoring system is installed inside the box body on the top end face of the temperature control component, a rotating mechanism is installed on the outer wall of the top end of the real-time monitoring system inside the box body, and a clamping component is installed inside the box body on the bottom end face of the rotating mechanism.

[0008] In one or more embodiments of the present invention, the box body includes three sets of storage compartments. An intermediate partition is fixedly connected between every two sets of storage compartments. A through hole matching the real-time monitoring system is opened in the center of the intermediate partition. A taking opening is opened on one side wall of each set of storage compartments, and an automatic door is installed on the inner wall of each set of storage compartments at the taking opening.

[0009] In one or more embodiments of the present invention, sealing strips are bonded to the outer surfaces of the automatic doors that fit onto the taking ports. A first rotating column is integrally formed on the bottom end surface of the automatic door. A rotating port matching the first rotating column is provided on the bottom end surface of the storage bin located at the taking port. A temperature sensor is installed on the inner wall of the automatic door close to the clamping assembly.

[0010] In one or more embodiments of the present invention, the temperature control assembly includes a temperature controller. A first heating rod, a second heating rod, and a third heating rod are respectively installed on the top end surface of the temperature controller from bottom to top on the inner wall of the storage bin.

[0011] In one or more embodiments of the present invention, the control and display system includes a PLC controller. An information control and display screen is fixedly connected to one side wall of the PLC controller. The real-time monitoring system includes a fixed column, and a number of monitoring cameras are installed on the outer wall of the fixed column.

[0012] In one or more embodiments of the present invention, the rotating mechanism includes a rotating motor. The rotating motor is fixedly connected to the inner wall of the top end of the storage bin. The output end of the rotating motor is fixedly connected to three groups of rotating rods. One end of each group of rotating rods is connected to each other and the angles are equal. A spur gear is rotatably connected to the bottom end surface of each group of rotating rods.

[0013] In one or more embodiments of the present invention, an internal gear is fixedly connected to the inner wall of the top end of the storage bin. The internal gear is simultaneously meshed with the three groups of spur gears. A rotating groove is provided on the bottom end surface of each group of spur gears, and a rotating bearing is installed on the inner wall of each rotating groove.

[0014] In one or more embodiments of the present invention, the clamping assembly includes a support top plate. The outer wall of the support top plate rotates on the inner wall of the rotating groove located at the rotating bearing. A heating and cooling integrated machine is installed on the bottom end surface of the support top plate, and a number of air outlets are provided on the bottom end surface of the heating and cooling integrated machine.

[0015] In one or more embodiments of the present invention, a support rod is integrally formed on the bottom end surface of the heating and cooling integrated machine. A number of support blocks are welded to the bottom end surface of the support rod. An expansion rod is fixedly connected to the bottom end surface of each of the number of support blocks. A support seat is fixedly connected to the bottom side wall of each of the number of expansion rods. A pair of clamping blocks is installed on one side wall of the support block above the support seat. A second rotating column is fixedly connected to one end of each pair of clamping blocks close to the support block. A coding plate is installed on the top end surface of each group of support blocks.

[0016] In one or more embodiments of the present invention, multiple layers of shells are placed above the support seat on the inner wall of the clamping block. An air inlet matching the air outlet is formed on the top end face of the multiple layers of shells. The air outlet is attached to the inner wall of the air inlet. The multiple layers of shells include a protective layer, a heating and cooling layer, and a cultivation layer. The air inlet communicates with the heating and cooling layer.

[0017] Compared with the prior art, a molecular breeding molecular marker kit of the present invention, through a multi-layer storage bin and a temperature control component with multiple temperatures, enables different molecules to be placed in different storage bins according to the suitable usage environment, greatly increasing the probability and efficiency of the success of molecular breeding experiments; by adding a control and display system and a real-time monitoring system, not only can the reagent with the corresponding number be quickly found, but also the state of the molecules in each reagent tube can be observed and recorded in real time, greatly increasing the experimental efficiency and promoting the sustainable development of agriculture. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments recorded in the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0019] Figure 1 Structural schematic of a molecular breeding molecular marker kit in an embodiment of the present invention Figure 1 ;

[0020] Figure 2 Structural schematic of a molecular breeding molecular marker kit in an embodiment of the present invention Figure 2 ;

[0021] Figure 3 For Figure 1 the enlarged view of part A in

[0022] Figure 4 Partial structural schematic of a molecular breeding molecular marker kit in an embodiment of the present invention Figure 1 ;

[0023] Figure 5 Partial structural schematic of a molecular breeding molecular marker kit in an embodiment of the present invention Figure 2 ;

[0024] Figure 6 Structural schematic of the rotating mechanism in an embodiment of the present invention Figure 1 ;

[0025] Figure 7Structural schematic of the rotating mechanism in an embodiment of the present invention Figure 2 ;

[0026] Figure 8 Structural schematic diagram of the clamping assembly in an embodiment of the present invention;

[0027] Figure 9 Top view cross-sectional view of the multi-layer housing in an embodiment of the present invention.

[0028] Main reference numerals description:

[0029] 1 - box body, 101 - storage bin, 102 - intermediate partition, 1021 - through hole, 103 - automatic door, 1031 - sealing strip, 1032 - first rotating column, 1033 - temperature sensor, 2 - temperature control assembly, 201 - temperature controller, 202 - first heating rod, 203 - second heating rod, 204 - third heating rod, 3 - control and display system, 301 - PLC controller, 302 - information control and display screen, 4 - real-time monitoring system, 401 - fixed column, 402 - monitoring camera, 5 - rotating mechanism, 501 - rotating motor, 502 - rotating rod, 503 - spur gear, 5031 - rotating groove, 504 - internal gear, 6 - clamping assembly, 601 - support top plate, 6011 - cooling and heating integrated machine, 6012 - air outlet, 602 - support rod, 603 - support block, 604 - telescopic rod, 605 - support seat, 606 - clamping block, 6061 - second rotating column, 607 - coding plate, 608 - multi-layer housing, 6081 - air inlet, 6082 - protective layer, 6083 - heating and cooling layer, 6084 - cultivation layer. Detailed implementation manners

[0030] In order to enable those skilled in the art to better understand the technical solutions in the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0031] As Figure 1As shown in the figure, a molecular breeding molecular marker kit in an embodiment of the present invention includes a box body 1. A temperature control component 2 is installed on the bottom end face of the box body 1. A control and display system 3 is installed on the top end face of the box body 1. A real-time monitoring system 4 is installed on the top end face of the temperature control component 2 inside the box body 1. A rotating mechanism 5 is installed inside the box body 1 above the real-time monitoring system 4. A clamping component 6 is installed on the bottom end face of the rotating mechanism 5 inside the box body 1. The box body 1 includes three groups of storage bins 101. Intermediate partitions 102 are fixedly connected between every two groups of storage bins 101. Through holes 1021 are opened in the middle of each group of intermediate partitions 102. A taking opening is opened on one side wall of each group of storage bins 101. An automatic door 103 is installed on the inner wall of each group of storage bins 101 at the taking opening. The temperature control component 2 is controlled by the control and display system 3 to adjust and monitor the temperature in different areas of the box body 1. According to the storage requirements of molecules, different molecules are placed in the storage bins 101 at different temperatures to avoid affecting the quality of molecular breeding due to the low external temperature. The rotating mechanism 5 is controlled by the control and display system 3 to work, driving the clamping component 6 to revolve and rotate simultaneously inside the box body 1, so that the internal molecules are evenly heated and the reaction efficiency is increased. The control and display system 3 and the real-time monitoring system 4 cooperate to observe the state of the molecular reagents inside the box body 1 in real time, quickly find the position of the reagent tube required by the staff, and monitor the remaining positions inside each layer of the storage bin 101 in real time, greatly increasing the convenience of molecular breeding molecular markers.

[0032] As Figure 2 shown, sealing strips 1031 are bonded to the outer walls of the automatic doors 103 that fit the taking openings. A first rotating column 1032 is integrally formed on the bottom end face of the automatic door 103. A rotating opening matching the first rotating column 1032 is opened on the bottom end face of the storage bin 101 at the taking opening. A temperature sensor 1033 is installed on the inner wall of the automatic door 103 close to the clamping component 6 to detect the temperature inside the kit at all times. A driving motor is installed on the inner wall of the rotating opening. The output end of the driving motor is connected to the first rotating column 1032. When the PLC controller controls the driving motor to work, the output end of the driving motor drives the first rotating column 1032 to rotate on the inner wall of the rotating opening, thereby realizing the opening of the automatic door 103.

[0033] As Figures 3 to 4As shown in the figure, the temperature control component 2 includes a temperature controller 201. The temperature controller 201 is connected to the PLC controller 301. By operating the PLC controller 301, the temperature inside the storage bin 101 can be observed and controlled. On the top end face of the temperature controller 201 and on the inner wall of the storage bin 101 from bottom to top, a first heating rod 202, a second heating rod 203, and a third heating rod 204 are respectively installed. The working temperature range of the first heating rod 202 is relatively low and is suitable for preheating and basic heating; the working temperature range of the second heating rod 203 is moderate and is suitable for fine adjustment and local heating; the working temperature range of the third heating rod 204 is relatively high and is suitable for high-temperature maintenance and rapid temperature rise. The control system will automatically adjust the heating power of each heating rod according to the difference between the set target temperature and the actual temperature to achieve precise temperature control and prevent overheating from damaging the equipment. A temperature protection mechanism is set. When the temperature exceeds the set safety range, the control system will automatically cut off the power supply of the heating rod or reduce the heating power to protect the safety of the reagent and the operator. The control and display system 3 includes a PLC controller 301. An information control and display screen 302 is fixedly connected to one side wall of the PLC controller 301. The real-time monitoring system 4 includes a fixed column 401. A number of monitoring cameras 402 are installed on the outer wall of the fixed column 401. The fixed column 401 and the monitoring cameras 402 are located in the middle of a number of reagent tubes. The clamping component 6 continuously drives the reagent tubes to rotate to monitor the status information of the reagent in real time and comprehensively, such as temperature, concentration, etc., and issue an early warning in case of an abnormality. The temperature, humidity, light and other parameters of the reagent storage environment are displayed in real time on the control interface, which helps researchers to understand the reagent status in time and avoid experiment interruption or failure. The system can analyze these data to help researchers better understand the experimental process and results.

[0034] As Figure 5 shown in the figure, the rotating mechanism 5 includes a rotating motor 501. The rotating motor 501 is fixedly connected to the top inner wall of the storage bin 101. The output end of the rotating motor 501 is fixedly connected with three rotating rods 502. One end of each group of rotating rods 502 is connected to each other and the angles are equal. A spur gear 503 is rotatably connected to the bottom end face of each group of rotating rods 502. An internal gear 504 is fixedly connected to the top inner wall of the storage bin 101. The internal gear 504 is meshed with the three spur gears 503 at the same time. A rotating groove 5031 is formed on the bottom end face of each group of spur gears 503. A rotating bearing is installed on the inner wall of each group of rotating grooves 5031. When the rotating motor 501 is working, it drives the three rotating rods 502 to rotate at the same time, so that the three rotating rods 502 drive the spur gears 503 to revolve and mesh with the internal gear 504 to achieve self-rotation.

[0035] As Figures 5 to 6As shown, the clamping assembly 6 includes a support top plate 601. The outer wall of the support top plate 601 rotates on the inner wall of the rotating groove 5031 of the rotating bearing. A heating and cooling integrated machine 6011 is installed on the bottom end face of the support top plate 601. A plurality of air outlets 6012 are provided on the bottom end face of the heating and cooling integrated machine 6011. A support rod 602 is integrally formed on the bottom end face of the support top plate 601. A plurality of support blocks 603 are welded on the bottom end face of the heating and cooling integrated machine 6011. An expansion rod 604 is fixedly connected to the bottom end face of each of the plurality of support blocks 603. A support seat 605 is fixedly connected to the side wall of the bottom end of each of the plurality of expansion rods 604. A pair of clamping blocks 606 are installed above the support seat 605 at one end of the support block 603. A second rotating column 6061 is fixedly connected to one end of each pair of clamping blocks 606 close to the support block 603. A coding plate 607 is installed on the top end face of each group of support blocks 603. A clamping motor is installed at one end of the support block 603 close to the clamping block 606. The output end of the clamping motor is connected to the second rotating column 6061. A multi-layer housing 608 is placed above the support seat 605 on the inner wall of the clamping block 606. An air inlet 6081 matching the air outlet 6012 is provided on the top end face of the multi-layer housing 608. The air outlet 6012 is attached to the inner wall of the air inlet 6081. During use, the clamping block 606 is in an open state. First, the multi-layer housing 608 is placed above the support seat 605, so that the support seat 605 plays a certain supporting role. An intelligent induction component is installed in the support seat 605. When the top end face of the support seat 605 senses weight, a signal is transmitted to the PLC controller 301. Subsequently, the PLC controller 301 drives the clamping motor to work, so that the clamping motor drives a pair of clamping blocks 606 on both sides of the multi-layer housing 608 to clamp the multi-layer housing 608. At the same time, the expansion rod 604 drives the multi-layer housing 608 and the multi-layer housing 608 to move upward, so that the inner wall of the air inlet 6081 fits onto the outer wall of the air outlet 6012, thus completing the fixation of the multi-layer housing 608. When wanting to take out the reagent with the corresponding code, input the corresponding code on the information control and display screen 302, and find the position of the reagent corresponding to the corresponding code through the monitoring system. Subsequently, the clamping assembly 6 drives the multi-layer housing 608 to rotate to one side of the automatic door 103. At the same time, the motor drives the automatic door 103 of the corresponding storage bin 101 to open. Subsequently, rotate the support block 603 to drive the support top plate 601 to rotate in the rotating bearing, and gently touch the bottom end face of the support seat 605. When the support seat 605 senses contact with an object, a signal is transmitted to the PLC controller 301. The PLC controller 301 controls the clamping motor to work, driving the clamping block 606 away from the outer wall of the multi-layer housing 608. At the same time, the expansion rod 604 works to drive the support seat 605 to move downward. Subsequently, after the staff takes the corresponding multi-layer housing 608 and moves it downward gently, the multi-layer housing 608 is moved away from the outside of the air outlet 6012. Subsequently, the multi-layer housing 608 can be taken out for subsequent experiments.

[0036] Further, anti-scratch pads are attached to the inner walls of the clamping block 606 and the support base 605 that are in contact with the multi-layer housing 608.

[0037] Preferably, the anti-scratch pad is made of aerogel, which has an extremely low density and excellent thermal insulation performance, and at the same time has the advantages of light weight, heat preservation and flexibility, avoiding the multi-layer housing 608 from being damaged by collision in the reagent kit.

[0038] As Figure 7 shown, the multi-layer housing 608 includes a protective layer 6082, a heating and cooling layer 6083, and a cultivation layer 6084. The air inlet 6081 communicates with the heating and cooling layer 6083. The protective layer 6082, the heating and cooling layer 6083, and the cultivation layer 6084 are closely attached to each other. The protective layer 6082 is made of boron-containing polyethylene composite material, which can efficiently attenuate γ-ray and thermal neutron radiation, protect the plant DNA sample from radiation damage, and can withstand the impact of a reagent tube falling from a height of 1.5 meters; the top of the heating and cooling layer 6083 communicates with the cooling and heating integrated machine 6011. A micro camera is installed on the inner wall of the heating and cooling layer 6083, which can more clearly observe the molecules in the reagent tube. When the external heating system is damaged or it is detected that a single reagent tube does not meet the temperature requirement and needs to be heated separately, the cooling and heating integrated machine 6011 can directly and quickly heat a single reagent tube to ensure the survival rate of the molecules. At the same time, when the temperature is too high and affects the molecular quality and needs to be quickly cooled down or a fire occurs, the cooling and heating integrated machine 6011 emits cold air to quickly cool down the molecules in the cultivation layer 6084 to ensure the survival quality of the molecules. The cultivation layer 6084 uses plexiglass, which has high transparency and chemical stability, facilitating the observation of the experimental process.

[0039] When in use, this molecular breeding kit integrates precise temperature control, intelligent monitoring, and efficient operation functions, significantly improving the experimental efficiency. It achieves multi-region temperature control through three independent storage bins 101. The first to third heating rods respectively meet the requirements of preheating, fine adjustment, and high temperature. The PLC controller 301 automatically adjusts the power to ensure precise temperature, and the protection mechanism prevents overheating risks. The rotating mechanism 5 drives the clamping component 6 to rotate both around its own axis and around a common axis, promoting uniform heating of the reagent and accelerating the reaction rate. The real-time monitoring system 4, together with the monitoring camera, comprehensively tracks the status of the reagent. The coding plate 607 is linked with the information control and display screen 302 to quickly locate the reagent, and the sealed design of the automatic door 103 ensures a stable environment. The multi-layer housing 608 structure combines a boron-containing polyethylene protective layer, an independent temperature-controlled heating layer, and a transparent cultivation layer, which not only protects the safety of the sample but also facilitates observation. The micro camera provides clear images at the molecular level. The innovative clamping component supports automatic induction opening and closing, and the aerogel anti-scratch pad avoids damage to the test tube. The cooling and heating integrated machine responds to sudden temperature anomalies to ensure the quality of molecular survival. This kit realizes the coordinated operation of temperature, movement, and monitoring through a highly integrated design, providing a safe, efficient, and intelligent cultivation process for molecular breeding.

[0040] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and without departing from the spirit or basic characteristics of the present invention, the present invention can be implemented in other specific forms. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present invention. Any reference signs in the claims should not be regarded as limiting the claimed rights.

[0041] In addition, it should be understood that although this specification is described according to embodiments, not every embodiment only contains an independent technical solution. This narrative way of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A molecular breeding molecular marker kit, characterized in that, It includes a box body, on the bottom end face of the box body, a temperature control component is installed, on the top end face of the box body, a control and display system is installed, on the top end face of the temperature control component and inside the box body, a real-time monitoring system is installed, inside the box body and on the outer wall at the top of the real-time monitoring system, a rotating mechanism is installed, and on the bottom end face of the rotating mechanism and inside the box body, a clamping component is installed.

2. The molecular breeding molecular marker kit according to claim 1, characterized in that The box body includes three storage bins, between every two of the storage bins, an intermediate partition is fixedly connected, in the center of the intermediate partition, a through hole matching the real-time monitoring system is provided, on one side wall of each storage bin, a taking opening is provided, and on the inner wall of each storage bin at the taking opening, an automatic door is installed.

3. The molecular breeding molecular marker kit according to claim 2, characterized in that, On the outer surface of the automatic door that fits to the taking opening, a sealing strip is bonded, on the bottom end face of the automatic door, a first rotating column is integrally formed, on the bottom end face of the storage bin at the taking opening, a rotating opening matching the first rotating column is provided, and on the inner wall of the automatic door close to the clamping component, a temperature sensor is installed.

4. A molecular breeding molecular marker kit according to claim 3, characterized in that, The temperature control component includes a temperature controller, on the top end face of the temperature controller and on the inner wall of the storage bin, a first heating rod, a second heating rod, and a third heating rod are installed from bottom to top respectively.

5. A molecular breeding molecular marker kit according to claim 4, characterized in that, The control and display system includes a PLC controller, on one side wall of the PLC controller, an information control and display screen is fixedly connected, the real-time monitoring system includes a fixed column, and on the outer wall of the fixed column, several groups of monitoring cameras are installed.

6. The molecular breeding molecular marker kit according to claim 5, characterized in that The rotating mechanism includes a rotating motor, the rotating motor is fixedly connected to the top inner wall of the storage bin, the output end of the rotating motor is fixedly connected to three rotating rods, one ends of each group of rotating rods are connected to each other and have equal angles, and on the bottom end face of each group of rotating rods, a spur gear is rotatably connected.

7. A molecular breeding molecular marker kit according to claim 6, characterized in that, On the top inner wall of the storage bin, an internal gear is fixedly connected, the internal gear is simultaneously meshed with the three spur gears, on the bottom end face of each group of spur gears, a rotating groove is provided, and on the inner wall of each rotating groove, a rotating bearing is installed.

8. A molecular breeding molecular marker kit according to claim 7, characterized in that, The clamping component includes a support top plate, the outer wall of the support top plate rotates on the inner wall of the rotating groove at the rotating bearing, on the bottom end face of the support top plate, a heating and cooling integrated machine is installed, and on the bottom end face of the heating and cooling integrated machine, several groups of air outlets are provided.

9. A molecular breeding molecular marker kit according to claim 8, wherein On the bottom end face of the heating and cooling integrated machine, a support rod is integrally formed, on the bottom end face of the support rod, several groups of support blocks are welded, on the bottom end face of each group of support blocks, a telescopic rod is fixedly connected, on the side wall at the bottom end of each group of telescopic rods, a support seat is fixedly connected, on the side wall of one end of the support block above the support seat, a pair of clamping blocks is installed, at one end of each pair of clamping blocks close to the support block, a second rotating column is fixedly connected, and on the top end face of each group of support blocks, a coding plate is installed.

10. A molecular breeding molecular marker kit according to claim 9, characterized in that, A plurality of layers of shells are placed above the support base on the inner wall of the clamping block. An air inlet matching the air outlet is formed on the top end surface of the plurality of layers of shells. The air outlet is attached to the inner wall of the air inlet. The plurality of layers of shells include a protective layer, a heating and cooling layer, and a cultivation layer. The air inlet communicates with the heating and cooling layer.

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