Isolated heart perfusion device

Abstract

The invention provides an isolated heart perfusion device, and relates to the technical field of heart perfusion equipment, the isolated heart perfusion device comprises an operation support table, the two sides of the top of the operation support table are fixedly connected with support vertical plates; the fixing structure is arranged between the supporting vertical plates and comprises a bearing concave frame which is arranged in the middle of the top of the operation supporting table, and extending convex plates are fixedly connected to the two sides of the bearing concave frame; the guide rod and the two-way screw rod are respectively arranged between the extending convex plates; the driven bevel gear is fixedly connected to one end of the bidirectional screw rod; the driving bevel gear is meshed with one side of the driven bevel gear, and a driving belt shaft is fixedly connected to the middle of the driving bevel gear; one end of the driving belt shaft is fixedly connected to the driving end of the first motor; the fixed clamping plates are arranged on two sides of the top of the bearing concave frame, and two ends of the fixed clamping plates are fixedly connected with connecting bent frames; a first screw hole and a guide sliding hole are respectively formed in the connecting bent frame; through cooperation of all the components, the device is of an integrated design, the integration degree is high, the function is complete, and the experiment operation efficiency is greatly improved.

Description

Technical Field

[0001] The present invention relates to the technical field of cardiac perfusion devices, and particularly to an ex vivo cardiac perfusion device. Background Art

[0002] Ex vivo cardiac perfusion is widely and deeply applied in scientific research. It is not only used to study the basic electrophysiological activities, metabolic processes of the heart, and the effects of drugs on cardiac function, but also plays an important role in the research of pathological mechanisms such as myocardial ischemia-reperfusion injury, arrhythmia, and heart failure. In addition, this technology also has an irreplaceable position in the cardiac safety evaluation of drugs. By simulating the in vivo environment, it evaluates the direct effects of drugs on the heart or the protective effects of drugs on myocardial injury, providing important reference basis for new drug research and development.

[0003] However, existing perfusion devices need to be equipped with precise peristaltic pumps, constant temperature water bath systems, and multi-channel biological signal analysis systems respectively in order to achieve precise control of parameters such as the pressure, flow rate, and temperature of the perfusion fluid, and to monitor and record various physiological parameters of the heart in real time. Currently, there is a lack of a functional and complete integrated ex vivo cardiac perfusion device, resulting in low experimental efficiency and difficult operation, which limits the efficiency of scientific research work. Summary of the Invention

[0004] In view of this, the present invention provides an ex vivo cardiac perfusion device. Through the setting of a fixed structure, it can not only avoid unnecessary movement of the heart during perfusion, prevent displacement of the heart from affecting the normal flow of the perfusion fluid, but also can be flexibly adjusted and fixed according to the sizes of hearts from different sources, so as to achieve precise adaptation, making the device applicable to perfusion experiments of various animal hearts and improving the applicability of the device. By setting a perfusion recovery component, it effectively avoids the pollution caused by direct discharge of the perfusion fluid, reduces the cleaning and maintenance costs. At the same time, on the one hand, it can also prevent impurities in the waste liquid from blocking the pipeline during the recovery process, and on the other hand, it can accurately analyze the content changes of various substances in the waste liquid, which helps to understand the material exchange situation during cardiac perfusion and provides more comprehensive data support for experimental research.

[0005] The present invention provides an ex vivo cardiac perfusion device, which specifically includes: an operation support platform, and two sides of the top of the operation support platform are fixedly connected with support vertical plates; A fixed structure, arranged between the support vertical plates, and this fixed structure includes: A bearing concave frame, arranged at the middle of the top of the operation support platform, and two sides of the bearing concave frame are fixedly connected with extension convex plates; A guide rod and a bidirectional lead screw, respectively arranged between the extension convex plates; A driven bevel gear, fixedly connected to one end of the bidirectional lead screw; The driving bevel gear meshes with one side of the driven bevel gear, and a driving belt shaft is fixedly connected to the middle thereof; The first motor, one end of the driving belt shaft is fixedly connected to its driving end; The fixed clamping plates are arranged on both sides of the top of the bearing concave frame, and connecting bent frames are fixedly connected to both ends thereof; The connecting bent frames are respectively provided with first screw holes and guiding sliding holes; The anti-slip pads are arranged on the inner side walls of the fixed clamping plates; The glass temperature control box is arranged outside the bearing concave frame. The bearing concave frame is fixedly connected to the inner side wall of the glass temperature control box through an extending convex plate. A water inlet is arranged at the top of one side of the glass temperature control box, and a water outlet is arranged at the bottom of the other side; Both ends of the top of the operation support platform are fixedly connected with fixed supports. The top of the fixed support is fixedly connected with a support frame. The top of the support frame is fixedly connected with a connecting top plate. A stabilizing angle block is fixedly installed between the support frame and the fixed support. A moving adjustment structure is arranged on the connecting top plate. The moving adjustment structure includes a rotating support plate and a moving belt block. The rotating support plate is fixedly connected to the connecting top plate. A first lead screw is rotatably installed between the rotating support plates. One end of the first lead screw is fixedly connected with a second motor. A moving belt block is arranged between the rotating support plates. A second screw hole is opened at the middle of the moving belt block. A limiting sliding plate is fixedly connected to the bottom of the moving belt block. At the same time, a limiting sliding groove is opened on the connecting top plate.

[0006] In at least some embodiments, the moving adjustment structure further includes a fixed concave frame and a moving carrier block. The bottom of the limiting sliding plate is fixedly connected with the fixed concave frame. A second lead screw is rotatably installed between the fixed concave frames. One end of the second lead screw is fixedly connected with a third motor. And a moving carrier block is arranged in the fixed concave frame. A third screw hole is opened on the moving carrier block. The third screw hole is rotationally meshed with the second lead screw.

[0007] In at least some embodiments, a guiding convex block is fixedly connected to the top of the moving carrier block. A guiding sliding groove is opened on the inner side of the bottom of the fixed concave frame. The guiding convex block is fitted and slidably installed in the guiding sliding groove. A fixed sliding frame is fixedly connected to the bottom of the moving carrier block. A square groove is opened on the fixed sliding frame.

[0008] In at least some embodiments, a moving carrier frame is arranged outside the fixed sliding frame. A slider is fixedly connected to the inner side of the top of the moving carrier frame. The slider is fitted and slidably installed in the square groove. A temperature controller is fixedly installed at the bottom of the moving carrier frame. A perfusion needle is installed at the bottom of the temperature controller. The temperature controller is electrically connected to a temperature sensor on one side. And an electric cylinder is fixedly installed at the bottom of the moving carrier block. The driving end of the electric cylinder is fixedly connected to the moving carrier frame.

[0009] In at least some embodiments, a perfusion recovery assembly 4 is provided on the connecting top plate 302. The perfusion recovery assembly 4 includes a peristaltic pump 401, a discharge pipe 402, and a reducing pipe 403. A peristaltic pump 401 is installed on the connecting top plate 302. One end of the peristaltic pump 401 is fixedly connected to one end of a heater 308 through an infusion pipe 4011, and the other end of the peristaltic pump 401 is fixedly connected to a liquid storage tank 4013 through a liquid extraction pipe 4012. A discharge pipe 402 is fixedly connected to the bearing recessed frame 201. The other end of the discharge pipe 402 is fixedly connected to a delivery pump 4021. The other end of the delivery pump 4021 is fixedly connected to a connecting elbow 4031 through a reducing pipe 403. A check valve 4032 is fixedly installed on the connecting elbow 4031. At the same time, the other end of the connecting elbow 4031 is fixedly connected to a recovery tank 407.

[0010] In at least some embodiments, an assembly arc groove 404 is formed on the connecting elbow 4031, and a clamping arc groove 4041 is formed on the inner side wall of the connecting elbow 4031. A filter screen 4042 is installed on the clamping arc groove 4041. An articulated frame 4043 is provided on the connecting elbow 4031 at one end of the assembly arc groove 404. A connecting convex block 4044 is fixedly connected to the connecting elbow 4031 at the other end of the assembly arc groove 404. A U-shaped groove 4045 is formed in the connecting convex block 4044, and a flipping groove 4046 is formed at one end of the connecting convex block 4044. A limiting hinge rod 4048 is hinged in the flipping groove 4046 through a first pin 4047.

[0011] In at least some embodiments, an assembly arc plate 405 is installed on the assembly arc groove 404. One end of the assembly arc plate 405 is fixedly connected to a hinge ear 4051, and the hinge ear 4051 is hingedly connected to the articulated frame 4043 through a second pin 4052. The other end of the assembly arc plate 405 is fixedly connected to an extension block 4053. A dislocation plate 4054 is fixedly connected to the bottom of the extension block 4053. A moving hole 4055 is formed in the dislocation plate 4054.

[0012] In at least some embodiments, a connecting rod 406 is movably installed in the moving hole 4055. One end of the connecting rod 406 is fixedly connected to a U-shaped plate 4061, and the other end of the connecting rod 406 is fixedly connected to a connecting handle 4062.

[0013] The in vitro heart perfusion device provided by the present invention has the following beneficial effects The present invention is provided with a fixing structure. By starting the first motor to drive the driving belt shaft to rotate, the driving belt shaft drives the driving bevel gear to rotate. The driving bevel gear meshes with the driven bevel gear to rotate, and the driven bevel gear drives the bidirectional lead screw to rotate. The bidirectional lead screw rotates and meshes with the first screw hole on the connecting bent frame. However, the other set of connecting bent frames is limited and guided on the guide rod through the guide sliding holes. Thus, the bidirectional lead screw drives the fixed clamping plates to move towards each other through the connecting bent frames, so as to clamp and fix the excised heart. This can not only avoid unnecessary movement of the heart during perfusion, prevent the displacement of the heart from affecting the normal flow of the perfusion fluid, but also can be flexibly adjusted according to the size of hearts from different sources for fixation, so as to achieve precise adaptation, making the device applicable to perfusion experiments of various animal hearts and improving the applicability of the device.

[0014] The present invention is provided with a moving and adjusting structure. By starting the second motor to drive the first lead screw to rotate, the first lead screw rotates and meshes with the second screw hole on the moving belt block, and then the perfusion needle can be vertically adjusted. By starting the third motor to drive the second lead screw to rotate, and then the second lead screw cooperates with the moving carrier block to drive the perfusion needle to be horizontally adjusted. Thus, after the heart is fixed, the position of the perfusion needle can be adjusted to align with the aorta, which is convenient for subsequent perfusion.

[0015] By providing a perfusion and recovery component, it effectively avoids the pollution caused by direct discharge of the perfusion fluid, reduces the cleaning and maintenance costs. At the same time, on the one hand, it can also prevent the impurities in the waste liquid from blocking the pipeline during the recovery process. On the other hand, it can accurately analyze the content changes of various substances in the waste liquid, which helps to understand the substance exchange situation during the heart perfusion process and provides more comprehensive data support for experimental research. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings of the embodiments will be briefly introduced below.

[0017] The following drawings in the description only relate to some embodiments of the present invention and do not limit the present invention.

[0018] In the drawings: Figure 1 The overall structural schematic diagram according to the present invention is shown; Figure 2 The structural schematic diagram of the glass temperature control box in the fixing structure according to the present invention is shown; Figure 3 The structural schematic diagram of some components of the fixing structure according to the present invention is shown; Figure 4 The schematic diagram of the moving and adjusting structure according to the present invention is shown; Figure 5 The schematic diagram of the horizontal moving structure of the moving and adjusting structure according to the present invention is shown; Figure 6 Shows a schematic diagram of the component structure of the mobile adjustment structure according to the present invention; Figure 7 Shows a schematic diagram of the perfusion recovery component according to the present invention; Figure 8 Shows a schematic diagram of the structure connecting the connecting elbow and the recovery tank in the perfusion recovery component according to the present invention; Figure 9 Shows according to the present invention Figure 8 The enlarged structure schematic diagram at position A; Figure 10 Shows a schematic diagram of the component structure of a part of the circulation filtration component according to the present invention; List of reference numerals 1. Operating support platform; 101. Support vertical plate; 2. Fixed structure; 201. Bearing concave frame; 2011. Extension convex plate; 2012. Guide rod; 2013. Bidirectional lead screw; 2014. Driven bevel gear; 202. Driving bevel gear; 2021. Driving belt shaft; 2022. First motor; 203. Fixed clamping plate; 2031. Connecting bent frame; 2032. First screw hole; 2033. Guide sliding hole; 204. Anti-slip pad; 205. Glass temperature control box; 2051. Water inlet; 2052. Water outlet; 3. Mobile adjustment structure; 301. Fixed support; 3011. Support frame; 3012. Stable corner block; 302. Connecting top plate; 3021. Rotating support plate; 3022. First lead screw; 3023. Second motor; 303. Moving belt block; 3031. Second screw hole; 3032. Limit sliding plate; 3033. Limit sliding groove; 304. Fixed concave frame; 3041. Second lead screw; 3042. Third motor; 305. Moving carrier block; 3051. Third screw hole; 3052. Guide convex block; 3053. Guide sliding groove; 3054. Fixed sliding frame; 3055. Square groove; 306. Moving carrier frame; 3061. Slide block; 307. Electric cylinder; 308. Temperature controller; 3081. Perfusion needle; 3082. Temperature sensor; 4. Perfusion recovery component; 401. Peristaltic pump; 4011. Infusion tube; 4012. Liquid extraction tube; 402, Drain pipe; 4021, Transfer pump; 403, Reducing pipe; 4031, Connecting elbow; 4032, Check valve; 404, Assembly arc groove; 4041, Clamping arc groove; 4042, Filter screen; 4043, Hinge bracket; 4044, Connecting bump; 4045, U-shaped groove; 4046, Flipping groove; 4047, First pin; 4048, Limit hinge rod; 405, Assembly arc plate; 4051, Hinge ear; 4052, Second pin; 4053, Extension block; 4054, Misaligned plate; 4055, Movable hole; 406, Connecting rod; 4061, U-shaped plate; 4062, Connecting handle; 407, Recovery tank.

[0019] Embodiment: Please refer to Figures 1 to 10 : The present invention provides an ex vivo heart perfusion device, including: an operation support platform 1, with support vertical plates 101 fixedly connected to both sides of its top; A fixing structure 2, arranged between the support vertical plates 101, and this fixing structure 2 includes: A bearing concave frame 201, fixed at the middle of the top of the operation support platform 1, with extension convex plates 2011 fixedly connected to both sides thereof; Guide rods 2012 and a bidirectional lead screw 2013, respectively arranged between the extension convex plates 2011; A driven bevel gear 2014, fixedly connected to one end of the bidirectional lead screw 2013; A driving bevel gear 202, meshing with one side of the driven bevel gear 2014, and a driving belt shaft 2021 fixedly connected to the middle thereof; A first motor 2022, with one end of the driving belt shaft 2021 fixedly connected to its driving end; Fixed clamping plates 203, arranged on both sides of the top of the bearing concave frame 201, with connecting bent frames 2031 fixedly connected to both ends thereof; The connecting bent frames 2031, respectively provided with first screw holes 2032 and guide sliding holes 2033 thereon; Anti-slip pads 204, arranged on the inner side walls of the fixed clamping plates 203; A glass temperature control box 205, arranged outside the bearing concave frame 201, and the bearing concave frame 201 is fixedly connected to the inner side wall of the glass temperature control box 205 through the extension convex plates 2011. A water inlet 2051 is provided at the top of one side of the glass temperature control box 205, and a water outlet 2052 is provided at the bottom of the other side; By starting the first motor 2022 to drive the drive belt shaft 2021 to rotate, the drive belt shaft 2021 drives the drive bevel gear 202 to rotate. The drive bevel gear 202 meshes with the driven bevel gear 2014 to rotate, and the driven bevel gear 2014 drives the bidirectional lead screw 2013 to rotate. The bidirectional lead screw 2013 rotates and meshes with the first screw hole 2032 on the connecting bent frame 2031. However, the other set of connecting bent frames 2031 is limited and guided on the guide rod 2012 through the guide sliding holes 2033. Thus, the bidirectional lead screw 2013 drives the fixed clamping plates 203 to move towards each other through the connecting bent frame 2031, thereby clamping and fixing the excised heart; the anti-abrasion pad 204 is used to prevent damage to the heart and ensure the safety of the excised heart during the fixing process; the glass temperature control box 205 can be heated and raised in temperature through a circulating water path to ensure the temperature around the heart.

[0020] Embodiment 2: On the basis of Embodiment 1, where as Figure 4 , Figure 5 and Figure 6As shown, both ends of the top of the operation support platform 1 are fixedly connected with fixed supports 301. The top of the fixed support 301 is fixedly connected with a support frame 3011. The top of the support frame 3011 is fixedly connected with a connecting top plate 302. A stable angle block 3012 is fixedly installed between the support frame 3011 and the fixed support 301. A moving and adjusting structure 3 is provided on the connecting top plate 302. The moving and adjusting structure 3 includes a rotating support plate 3021 and a moving belt block 303. The connecting top plate 302 is fixedly connected with a rotating support plate 3021. A first lead screw 3022 is rotatably installed between the rotating support plates 3021. One end of the first lead screw 3022 is fixedly connected with a second motor 3023. A moving belt block 303 is provided between the rotating support plates 3021. A second screw hole 3031 is opened at the middle of the moving belt block 303. The bottom of the moving belt block 303 is fixedly connected with a limiting sliding plate 3032. At the same time, a limiting sliding groove 3033 is opened on the connecting top plate 302. The moving and adjusting structure 3 further includes a fixed concave frame 304 and a moving carrier block 305. The bottom of the limiting sliding plate 3032 is fixedly connected with a fixed concave frame 304. A second lead screw 3041 is rotatably installed between the fixed concave frames 304. One end of the second lead screw 3041 is fixedly connected with a third motor 3042. And a moving carrier block 305 is provided in the fixed concave frame 304. A third screw hole 3051 is opened on the moving carrier block 305. The third screw hole 3051 is rotationally engaged with the second lead screw 3041. The top of the moving carrier block 305 is fixedly connected with a guiding convex block 3052. A guiding sliding groove 3053 is opened at the inner bottom of the fixed concave frame 304. The guiding convex block 3052 is fitted and slidably installed in the guiding sliding groove 3053. The bottom of the moving carrier block 305 is fixedly connected with a fixed sliding frame 3054. A square groove 3055 is opened on the fixed sliding frame 3054. A moving carrier frame 306 is provided outside the fixed sliding frame 3054. The inner top of the moving carrier frame 306 is fixedly connected with a slider 3061. The slider 3061 is fitted and slidably installed in the square groove 3055. A temperature controller 308 is fixedly installed at the bottom of the moving carrier frame 306. A perfusion needle 3081 is installed at the bottom of the temperature controller 308. The temperature controller 308 is electrically connected to a temperature sensor 3082 on one side. And an electric cylinder 307 is fixedly installed at the bottom of the moving carrier block 305. The driving end of the electric cylinder 307 is fixedly connected with the moving carrier frame 306; By starting the second motor 3023 to drive the first lead screw 3022 to rotate, the first lead screw 3022 is rotationally engaged with the second screw hole 3031 on the moving belt block 303, and then the perfusion needle 3081 can be vertically adjusted. By starting the third motor 3042 to drive the second lead screw 3041 to rotate, the second lead screw 3041 cooperates with the moving carrier block 305 to drive the perfusion needle 3081 to be horizontally adjusted. Then, after the heart is fixed, the position of the perfusion needle 3081 can be adjusted to align with the aorta, facilitating subsequent perfusion. The temperature controller 308 can ensure that the perfusion fluid is at an appropriate temperature, avoiding the influence of low temperature of the perfusion fluid on cell activity. Then, start the electric cylinder 307 to push the moving carrier frame 306 to move, and the moving carrier frame 306 drives the perfusion needle 3081 into the aorta. The temperature controller 308 can arbitrarily control the temperature of the perfusion fluid, and at the same time, the temperature change of the perfusion fluid can be displayed on the temperature controller 308. The function of the temperature sensor 3082 is to monitor the temperature of the glass temperature control box 205, prevent the temperature around the heart from becoming low, and at the same time, its temperature can also be displayed on the temperature controller 308.

[0021] Embodiment 3: On the basis of Embodiment 1, as Figures 7 to 10 shown, a perfusion and recovery assembly 4 is provided on the connecting top plate 302. The perfusion and recovery assembly 4 includes a peristaltic pump 401, a discharge pipe 402, and a reducing pipe 403. The peristaltic pump 401 is installed on the connecting top plate 302. One end of the peristaltic pump 401 is fixedly connected to one end of the heater 308 through an infusion pipe 4011, and the other end of the peristaltic pump 401 is fixedly connected to a liquid storage tank 4013 through a liquid extraction pipe 4012. The discharge pipe 402 is fixedly connected to the bearing concave frame 201. The other end of the discharge pipe 402 is fixedly connected to a delivery pump 4021. The other end of the delivery pump 4021 is fixedly connected to a connecting elbow 4031 through a reducing pipe 403. A check valve 4032 is fixedly installed on the connecting elbow 4031. At the same time, the other end of the connecting elbow 4031 is fixedly connected to a recovery tank 407.

[0022] An assembly arc groove 404 is provided on the connecting elbow 4031. A clamping arc groove 4041 is provided on the inner side wall of the connecting elbow 4031. A filter screen 4042 is installed on the clamping arc groove 4041. One end of the assembly arc groove 404 on the connecting elbow 4031 is provided with a hinge bracket 4043. The other end of the assembly arc groove 404 on the connecting elbow 4031 is fixedly connected to a connecting convex block 4044. A U-shaped groove 4045 is provided on the connecting convex block 4044, and a flipping groove 4046 is provided at one end of the connecting convex block 4044. A limiting hinge rod 4048 is hinged in the flipping groove 4046 through a first pin 4047.

[0023] An assembly arc plate 405 is installed on the assembly arc groove 404. One end of the assembly arc plate 405 is fixedly connected with a hinge ear 4051. The hinge ear 4051 is hingedly connected with a hinge frame 4043 through a second pin 4052. The other end of the assembly arc plate 405 is fixedly connected with an extension block 4053. The bottom of the extension block 4053 is fixedly connected with a misalignment plate 4054. An activity hole 4055 is formed in the misalignment plate 4054.

[0024] A connecting rod 406 is movably installed in the activity hole 4055. One end of the connecting rod 406 is fixedly connected with a U-shaped plate 4061. The other end of the connecting rod 406 is fixedly connected with a connecting handle 4062.

[0025] By starting the peristaltic pump 401, the perfusion fluid is transported into the heater 308 through the infusion tube 4011 to prevent the low temperature of the perfusion fluid from affecting the cell activity. Then the perfusion fluid enters the connecting elbow 4031 through the discharge pipe 402, and the waste liquid is filtered by the filter screen 4042. Finally, the waste liquid flows into the recovery tank 407 to prevent impurities from blocking the pipeline during the recovery process and avoid impurities affecting the subsequent analysis and treatment of the waste liquid. Then, by flipping the limit hinge rod 4048, then rotating the connecting handle 4062 to make the U-shaped plate 4061 horizontal, and then it can be pulled out, so that the assembly arc plate 405 can be opened, and the filter screen 4042 can be disassembled and replaced. During installation, only need to snap the filter screen 4042 into the snap-in arc groove 4041, then flip the assembly arc plate 405, so that the connecting convex block 4044 is aligned with the misalignment plate 4054, then rotate the connecting handle 4062 to make the U-shaped plate 4061 horizontal, and then push the connecting handle 4062 to make the U-shaped plate 4061 pass through the U-shaped groove 4045 to lift the limit hinge rod 4048. Under the principle of unbalanced gravity, the U-shaped plate 4061 automatically resets downward, and then flip down the limit hinge rod 4048 to complete the limitation of the U-shaped plate 4061, thus completing the installation of the assembly arc plate 405.

[0026] Specific usage and function of this embodiment: In the present invention, first, the perfusion needle 3081 is moved to the outside by moving the adjustment structure 3, so that the heart can be hung on the perfusion needle 3081 conveniently, and then the isolated heart is taken out with the top of the heart facing upwards, and then the position of the perfusion needle 3081 is aligned with the aorta, so that the blood vessels of the heart are wrapped around the outside of the perfusion needle 3081, and then it is tied to the perfusion needle 3081 with a rope. At this time, the glass temperature control box 205 is adjusted to a suitable temperature for the heart, and then the heart is placed in the supporting recessed frame 201 by moving the adjustment structure 3, and then the first motor 2022 is started to drive the driving belt shaft 2021 to rotate, the driving belt shaft 2021 drives the driving bevel gear 202 to rotate, the driving bevel gear 202 engages with the driven bevel gear 2014 to rotate, the driven bevel gear 2014 drives the bidirectional screw rod 2013 to rotate, and the bidirectional screw rod 2013 is connected to the first screw hole 2032 on the curved frame 2031 The heart is then rotated and engaged, while another set of connecting bends 2031 is guided by the upper limit position of the guide rod 2012 through the guide slide hole 2033, so that the bidirectional screw rod 2013 drives the fixed splint 203 to move toward each other through the connecting bends 2031, thereby clamping and fixing the isolated heart, so that it can avoid unnecessary shaking and movement of the heart during the perfusion process, and prevent the displacement of the heart from affecting the normal circulation of the perfusion fluid. Then, the peristaltic pump 401 is started to deliver the perfusion fluid to the temperature controller 308 through the infusion tube 4011. Under the temperature adjustment function of the temperature controller 308, the temperature of the perfusion fluid is guaranteed to be within a temperature range that will not affect the cell activity, and finally enters the heart through the perfusion needle 3081. The waste liquid after perfusion flows into the bearing recessed frame 201, and the delivery pump 4021 is started to extract the waste liquid through the discharge pipe 402, and then it is delivered to the connecting bend cylinder 4031 through the reducer 403, and finally flows into the recovery tank 407 after filtration and purification.

Claims

1. An ex vivo heart perfusion device, comprising: Operating support platform (1), with support vertical plates (101) fixedly connected to both sides of its top; characterized in that: Fixing structure (2), arranged between the support vertical plates (101), and this fixing structure (2) includes: Carrying concave frame (201), arranged at the middle of the top of the operating support platform (1), and extension convex plates (2011) are fixedly connected to both sides thereof; Guide rods (2012) and bidirectional lead screws (2013), respectively arranged between the extension convex plates (2011); Driven bevel gear (2014), fixedly connected to one end of the bidirectional lead screw (2013); Driving bevel gear (202), meshing with one side of the driven bevel gear (2014), and a driving belt shaft (2021) is fixedly connected to the middle thereof; First motor (2022), one end of the driving belt shaft (2021) is fixedly connected to its driving end; Fixing clamping plates (203), arranged on both sides of the top of the carrying concave frame (201), and connecting bent frames (2031) are fixedly connected to both ends thereof; Connecting bent frames (2031), on which first screw holes (2032) and guide sliding holes (2033) are respectively opened; Anti-slip pads (204), arranged on the inner side walls of the fixing clamping plates (203); Glass temperature control box (205), arranged outside the carrying concave frame (201), the carrying concave frame (201) is fixedly connected to the inner side wall of the glass temperature control box (205) through the extension convex plates (2011), a water inlet (2051) is arranged at the top of one side of the glass temperature control box (205), and a water outlet (2052) is arranged at the bottom of the other side; Fixed supports (301) are fixedly connected to both ends of the top of the operating support platform (1), a support frame (3011) is fixedly connected to the top of the fixed support (301), a connecting top plate (302) is fixedly connected to the top of the support frame (3011), a stabilizing angle block (3012) is fixedly installed between the support frame (3011) and the fixed support (301), a moving adjustment structure (3) is arranged on the connecting top plate (302), and the moving adjustment structure (3) includes a rotating support plate (3021) and a moving belt block (303). A rotating support plate (3021) is fixedly connected to the connecting top plate (302), a first lead screw (3022) is rotatably installed between the rotating support plates (3021), one end of the first lead screw (3022) is fixedly connected to a second motor (3023), a moving belt block (303) is arranged between the rotating support plates (3021), a second screw hole (3031) is opened at the middle of the moving belt block (303), a limiting sliding plate (3032) is fixedly connected to the bottom of the moving belt block (303), and at the same time, a limiting sliding groove (3033) is opened on the connecting top plate (302).

2. The ex vivo heart perfusion device according to claim 1, wherein: The mobile adjustment structure (3) further includes a fixed concave frame (304) and a mobile carrier block (305). The bottom of the limit slide plate (3032) is fixedly connected to the fixed concave frame (304). A second lead screw (3041) is rotatably installed between the fixed concave frames (304). One end of the second lead screw (3041) is fixedly connected to a third motor (3042). And a mobile carrier block (305) is provided in the fixed concave frame (304). A third screw hole (3051) is formed in the mobile carrier block (305). The third screw hole (3051) is rotationally engaged with the second lead screw (3041).

3. The ex vivo heart perfusion device according to claim 2, wherein: A guide convex block (3052) is fixedly connected to the top of the mobile carrier block (305). A guide chute (3053) is formed in the inner bottom of the fixed concave frame (304). The guide convex block (3052) is fitted and slidably installed in the guide chute (3053). A fixed sliding frame (3054) is fixedly connected to the bottom of the mobile carrier block (305). A square groove (3055) is formed in the fixed sliding frame (3054).

4. An ex vivo heart perfusion device according to claim 3, characterized in that: A mobile carrier frame (306) is provided outside the fixed sliding frame (3054). A slider (3061) is fixedly connected to the inner top of the mobile carrier frame (306). The slider (3061) is fitted and slidably installed in the square groove (3055). A temperature controller (308) is fixedly installed at the bottom of the mobile carrier frame (306). A perfusion needle (3081) is installed at the bottom of the temperature controller (308). The temperature controller (308) is electrically connected to a temperature sensor (3082) on one side. And an electric cylinder (307) is fixedly installed at the bottom of the mobile carrier block (305). The driving end of the electric cylinder (307) is fixedly connected to the mobile carrier frame (306).

5. The ex vivo heart perfusion device according to claim 1, wherein: A perfusion recovery assembly (4) is provided on the connection top plate (302). The perfusion recovery assembly (4) includes a peristaltic pump (401), a discharge pipe (402) and a reducing pipe (403). A peristaltic pump (401) is installed on the connection top plate (302). One end of the peristaltic pump (401) is fixedly connected to one end of a heater (308) through an infusion pipe (4011). The other end of the peristaltic pump (401) is fixedly connected to a liquid storage tank (4013) through a liquid extraction pipe (4012). A discharge pipe (402) is fixedly connected to the bearing concave frame (201). The other end of the discharge pipe (402) is fixedly connected to a delivery pump (4021). The other end of the delivery pump (4021) is fixedly connected to a connection elbow (4031) through a reducing pipe (403). A check valve (4032) is fixedly installed on the connection elbow (4031). At the same time, the other end of the connection elbow (4031) is fixedly connected to a recovery tank (407).

6. The ex vivo heart perfusion device according to claim 5, wherein: An assembly arc groove (404) is formed on the connecting elbow (4031). A clamping arc groove (4041) is formed on the inner side wall of the connecting elbow (4031). A filter screen (4042) is installed on the clamping arc groove (4041). An articulated frame (4043) is provided on one end of the connecting elbow (4031) where the assembly arc groove (404) is located. A connecting convex block (4044) is fixedly connected to the other end of the connecting elbow (4031) where the assembly arc groove (404) is located. A U-shaped groove (4045) is formed on the connecting convex block (4044). One end of the connecting convex block (4044) is provided with a flipping groove (4046). A limiting hinge rod (4048) is hinged in the flipping groove (4046) through a first pin (4047).

7. An ex vivo heart perfusion device according to claim 6, characterized in that: An assembly arc plate (405) is installed on the assembly arc groove (404). One end of the assembly arc plate (405) is fixedly connected with a hinge ear (4051). The hinge ear (4051) is hingedly connected to the articulated frame (4043) through a second pin (4052). The other end of the assembly arc plate (405) is fixedly connected with an extension block (4053). A misaligned plate (4054) is fixedly connected to the bottom of the extension block (4053). A movable hole (4055) is formed on the misaligned plate (4054).

8. An ex vivo heart perfusion device according to claim 7, characterized in that: A connecting rod (406) is movably installed in the movable hole (4055). One end of the connecting rod (406) is fixedly connected with a U-shaped plate (4061). The other end of the connecting rod (406) is fixedly connected with a connecting handle (4062).

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