Application of migratory locust epidermis test probe and test method

Abstract

The invention relates to the technical field of migratory locust epidermis test probes, in particular to application of a probe to determination of mechanical indexes of fracture displacement corresponding to efficiency, internodal membrane fracture strength and maximum tensile force of a target gene in the insect development process. A migratory locust epidermis testing probe comprises a stretching probe and a puncturing probe, the stretching probe comprises a clamp base, two sets of clamp bases are arranged at the top end of the clamp base oppositely, a knob bolt is rotationally connected to the middle of the right side wall of each clamp base, and springs are arranged on the upper side and the lower side of each knob bolt correspondingly; a plugging screw is mounted on the right side of the spring; the tensile probe is used for measuring mechanical indexes such as the maximum tensile force, the breaking strength and the breaking displacement corresponding to the maximum tensile force of the migratory locust internode membrane under the action of external force, so that people can more intuitively perceive the tensile property of the migratory locust internode membrane; and the maximum force value when the probe penetrates through the sample can be measured through the puncture probe of the texture analyzer, and the specific hardness value is obtained.

Description

Technical Field

[0001] The present invention relates to the technical field of locust epidermis test probes, and in particular to an application and test method for a locust epidermis test probe. Background Art

[0002] When conducting research on insects, different probes need to be selected for testing according to different research directions of locusts. For example, in order to test mechanical indexes such as the maximum tensile force of the intersegmental membrane of locusts, the fracture strength, and the fracture displacement corresponding to the maximum tensile force, a tensile probe is selected for detection; when it is necessary to quantify the hardness change of the locust ovipositor growing daily, a puncture probe is used for detection.

[0003] Most of the existing tensile probes are mostly operated manually by holding or pulling a pressure gauge and adding a needle to puncture, with a large artificial operation space, resulting in low fastening performance of the tensile probe, making the locust epidermis prone to detachment from the probe or sliding during the test, reducing the accuracy of test data; at the same time, when detecting the locust ovipositor, most of them are detected by a single puncture probe, resulting in messy data.

[0004] Therefore, aiming at the problem that most of the above-mentioned probes are mostly operated manually by holding or pulling a pressure gauge and adding a needle to puncture, with a large artificial operation space and reducing the accuracy of test data, an application and test method for a locust epidermis test probe can be designed. Summary of the Invention

[0005] In order to overcome the problem that most of the probes are mostly operated manually by holding or pulling a pressure gauge and adding a needle to puncture, with a large artificial operation space and reducing the accuracy of test data.

[0006] The technical solution of the present invention is: an application of a probe in determining the efficacy of a target gene during insect development and the mechanical indexes such as the fracture strength of the intersegmental membrane and the fracture displacement corresponding to the maximum tensile force.

[0007] Preferably, a tensile probe is used to measure mechanical indexes such as the maximum tensile force of the locust intersegmental membrane, the fracture strength, and the fracture displacement corresponding to the maximum tensile force under an external force, making people's perception of the tensile performance of the locust intersegmental membrane more intuitive; the maximum force value when the probe penetrates the sample can be measured by the puncture probe of the texture analyzer, so as to obtain the specific hardness value of the sample.

[0008] Preferably, it includes a tensile probe and a puncture probe. The tensile probe includes a fixture base, and a fixture seat is arranged at the top of the fixture base, and there are two groups of relatively arranged fixture seats. A knob bolt is rotatably connected to the middle position of the right side wall of the fixture seat, springs are arranged on both the upper and lower sides of the knob bolt, and a set screw is installed on the right side of the spring;

[0009] The puncture probe includes an HPD test platform, and support columns are arranged at both the left and right ends of the bottom of the HPD test platform.

[0010] Preferably, a friction fixing seat is movably connected to the middle position inside the fixture seat, and the right side of the friction fixing seat is bolted to the knob. A friction plate is installed on the left side inside the fixture seat, and the friction fixing seat and the friction plate are movably connected to each other.

[0011] Preferably, a locking nut is rotatably connected to the middle position at the top of the upper fixture seat, and an M6 set screw is threadedly connected to the middle position inside the locking nut.

[0012] Preferably, a texture analyzer is provided at the middle position at the top of the HPD test platform.

[0013] Preferably, a slide is installed at the middle position at the top of the texture analyzer on the top of the HPD test platform.

[0014] Preferably, an extension rod is provided at the upper end of the slide, a probe is installed at the bottom end of the extension rod, and the probe is vertically arranged above the slide.

[0015] A test method for a test probe for locust epidermis testing, which includes a test probe for locust epidermis testing as described above, and the steps are as follows:

[0016] First step, select healthy and intact locust samples, take their lower intersegmental membrane samples to ensure the integrity of the samples, and cut the locust intersegmental membrane samples into test shapes and sizes through cutting tools;

[0017] Second step, conduct a tensile test on the locust intersegmental membrane specimen: install the tensile probe into the M6 set screw, rotate the locking nut to lock the tensile probe in the M6 set screw, fix the locust intersegmental membrane specimen on the friction fixing seat and the friction plate, rotate the knob bolt to tighten the locust intersegmental membrane specimen, and adjust the position of the tensile probe so that it is in the vertical direction of the locust intersegmental membrane specimen;

[0018] Third step, adjust the tensile parameters or test range of the tensile equipment as needed, turn on the tensile test equipment, apply an external force to gradually stretch the locust intersegmental membrane specimen, and when the specimen breaks, the tensile equipment stops stretching, and organize and calculate the tensile data;

[0019] Fourth step, conduct a puncture test on the ovipositor of the locust through a texture analyzer: when the locust molts, regularly collect its ovipositors at different time points as samples; fix the ovipositor on the slide through an adhesive, and install the probe on the extension rod;

[0020] Fifth step, start the texture analyzer to make the extension rod puncture the ovipositor, and the texture analyzer records the change in the puncture force value of the extension rod. When the probe reaches the puncture distance or the maximum force value, the texture analyzer automatically stops measuring, and repeat the measurement for multiple samples at each time point.

[0021] Advantages of the present invention: The stretching probe measures mechanical indexes such as the maximum tensile force, breaking strength, and breaking displacement corresponding to the maximum tensile force of the intersegmental membrane of locusts under external force, making people's perception of the stretching performance of the locust intersegmental membrane more intuitive; the clamping action of the stretching probe is completed by the clamp tightened by the lock nut, so that the locust epidermis will not break away from the probe or slip during the test, ensuring the accuracy of the test data; at the same time, the vertical distance of the stretching probe can be infinitely close, ensuring that the locust intersegmental membrane will not deform during the fixation of the sample, resulting in data distortion; and the maximum force value when the probe penetrates the sample can be measured by the puncture probe of the texture analyzer, so as to obtain the specific hardness value of the sample. At the same time, by comparing the hardness differences of the ovipositors of wild insects and gene-treated insects in the same period, the efficacy of the target gene during insect development can be determined. Description of the Drawings

[0022] Figure 1 Shown is a three-dimensional structural schematic diagram of the stretching probe fixture for the locust epidermis test probe of the present invention;

[0023] Figure 2 Shown is a front view structural schematic diagram of the fixture for the locust epidermis test probe of the present invention;

[0024] Figure 3 Shown is a top view structural schematic diagram of the fixture for the locust epidermis test probe of the present invention;

[0025] Figure 4 Shown is a three-dimensional structural schematic diagram of the puncture probe for the locust epidermis test probe of the present invention;

[0026] Figure 5 Shown is a front view structural schematic diagram of the puncture probe for the locust epidermis test probe of the present invention;

[0027] Figure 6 Shown is a top view structural schematic diagram of the puncture probe for the locust epidermis test probe of the present invention.

[0028] Description of the reference numerals: 1. Fixture base; 2. Fixture seat; 3. Knob bolt; 4. Spring; 5. Set screw; 6. Friction fixing seat; 7. Friction plate; 8. Lock nut; 9. M6 set screw; 10. HPD test platform; 11. Slide; 12. Extension rod; 13. Probe. Detailed Embodiments

[0029] The present invention will be further described below in conjunction with the drawings and embodiments.

[0030] The present invention provides an embodiment: an application of a probe in determining the efficacy of a target gene during insect development and the mechanical indexes of the breaking strength of the intersegmental membrane and the breaking displacement corresponding to the maximum tensile force.

[0031] Please refer to Figures 1 - 3 , in this embodiment, it includes a stretching probe and a puncture probe. The stretching probe includes a fixture base 1. At the top of the fixture base 1, there is a fixture seat 2, and there are two sets of fixture seats 2 arranged oppositely. At the middle position of the right side wall of the fixture seat 2, there is a rotary bolt 3 rotatably connected. On both the upper and lower sides of the rotary bolt 3, there are springs 4, and on the right side of the springs 4, there is a plug screw 5 installed; at the middle position inside the fixture seat 2, there is a friction fixing seat 6 movably connected, and the right side of the friction fixing seat 6 is connected to the rotary bolt 3. On the left side inside the fixture seat 2, there is a friction plate 7 installed, and the friction fixing seat 6 and the friction plate 7 are movably connected; at the middle position of the top of the upper fixture seat 2, there is a lock nut 8 rotatably connected, and at the middle position inside the lock nut 8, there is an M6 set screw 9 threadedly connected.

[0032] Example 1

[0033] Select healthy and complete locust samples, take their lower intersegmental membrane samples, ensure the integrity of the samples, and cut the locust intersegmental membrane samples into test shapes and sizes through cutting tools; conduct tensile tests on the locust intersegmental membrane specimens: Install the stretching probe into the M6 set screw 9, rotate the lock nut 8 to lock the stretching probe inside the M6 set screw 9, fix the locust intersegmental membrane specimens on the friction fixing seat 6 and the friction plate 7, rotate the rotary bolt 3 to tighten the locust intersegmental membrane specimens, adjust the position of the stretching probe so that it is in the vertical direction of the locust intersegmental membrane specimens; adjust the stretching parameters or test range of the stretching equipment as needed, turn on the stretching test equipment, apply an external force to gradually stretch the locust intersegmental membrane specimens. When the specimens are stretched until they break, the stretching equipment stops stretching, and organize and calculate the stretching data.

[0034] Please refer to Figures 4 - 6 , in this embodiment, the puncture probe includes an HPD test platform 10, and at both the left and right ends of the bottom of the HPD test platform 10, there are support columns. At the middle position of the top of the HPD test platform 10, there is a texture analyzer; at the middle position of the top of the texture analyzer on the top of the HPD test platform 10, there is a slide 11 installed; at the upper end of the slide 11, there is an extension rod 12, and at the bottom of the extension rod 12, there is a probe 13 installed, and the probe 13 is vertically arranged above the slide 11.

[0035] Example 2

[0036] Adjust the stretching parameters or test range of the stretching device as needed, turn on the stretching test device, apply an external force to gradually stretch the locust intersegmental membrane specimen. When the specimen breaks, the stretching device stops stretching, and organize and calculate the stretching data; perform puncture detection on the ovipositor of the locust through a texture analyzer: when the locust molts, regularly collect its ovipositors at different time points as samples; fix the ovipositor on the slide 11 through an adhesive, and install the probe 13 on the extension rod 12; start the texture analyzer to make the extension rod 12 puncture the ovipositor, and the texture analyzer records the change in the puncture force value of the extension rod 12. When the probe reaches the puncture distance or the maximum force value, the texture analyzer automatically stops measuring, and repeat the measurement for multiple samples at each time point.

[0037] Through the above steps, the stretching probe is used to measure mechanical indexes such as the maximum tensile force, breaking strength, and breaking displacement corresponding to the maximum tensile force of the locust intersegmental membrane under the action of an external force, making people's perception of the stretching performance of the locust intersegmental membrane more intuitive; and the maximum force value when the probe passes through the sample can be measured through the puncture probe of the texture analyzer, so as to obtain the specific hardness value of the sample. At the same time, compare the hardness differences of the ovipositors of wild insects and genetically modified insects at the same time period to determine the efficacy of the target gene during insect development; to solve the problem that most probes are punctured by hand-held or pulling the pressure gauge needle, with a large artificial operation space, reducing the accuracy of the test data.

[0038] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the scope of knowledge possessed by those skilled in the art.

Claims

1. Application of a probe in determining the mechanical indexes of the efficacy of a target gene during insect development, the fracture strength of the intersegmental membrane, and the fracture displacement corresponding to the maximum tensile force.

2. A test probe for the cuticle of Locusta migratoria, characterized in that: It includes a tensile probe and a puncture probe. The tensile probe includes a fixture base (1). At the top of the fixture base (1), there is a fixture seat (2), and there are two sets of relatively arranged fixture seats (2). At the middle position of the right side wall of the fixture seat (2), there is a rotatable knob bolt (3). Springs (4) are arranged on both the upper and lower sides of the knob bolt (3), and a set screw (5) is installed on the right side of the spring (4). The puncture probe includes an HPD test platform (10), and support columns are arranged at both the left and right ends of the bottom of the HPD test platform (10).

3. The test probe for the epidermis of Locusta migratoria according to claim 2, wherein: A friction fixing seat (6) is movably connected to the middle position inside the fixture seat (2), and the right side of the friction fixing seat (6) is connected to the knob bolt (3). A friction plate (7) is installed on the left side inside the fixture seat (2), and the friction fixing seat (6) is movably connected to the friction plate (7).

4. The epidermal test probe for locusts according to claim 2, characterized in that: At the middle position of the top of the upper fixture seat (2), there is a rotatable lock nut (8), and an M6 set screw (9) is threadedly connected to the middle position inside the lock nut (8).

5. The test probe for the epidermis of Locusta migratoria according to claim 2, wherein: A texture analyzer is arranged at the middle position of the top of the HPD test platform (10).

6. The epidermal test probe for locusts according to claim 5, characterized in that: A specimen slide (11) is installed at the middle position of the top of the texture analyzer on the top of the HPD test platform (10).

7. The epidermal test probe for locusts according to claim 6, characterized in that: An extension rod (12) is arranged at the upper end of the specimen slide (11), and a probe (13) is installed at the bottom end of the extension rod (12), and the probe (13) is vertically arranged above the specimen slide (11).

8. A test method for a test probe of the locust cuticle, characterized in that: It includes a probe for locust cuticle testing according to claims 2-7, and the steps are as follows: First step, select healthy and intact locust samples, and take their lower intersegmental membrane samples to ensure the integrity of the samples. Cut the locust intersegmental membrane samples into test shapes and sizes through cutting tools. Second step, conduct tensile detection on the locust intersegmental membrane specimens: Install the tensile probe into the M6 set screw (9), rotate the lock nut (8) to lock the tensile probe in the M6 set screw (9), fix the locust intersegmental membrane specimens on the friction fixing seat (6) and the friction plate (7), rotate the knob bolt (3) to tighten the locust intersegmental membrane specimens, and adjust the position of the tensile probe to make it in the vertical direction of the locust intersegmental membrane specimens. Third step, adjust the tensile parameters or test range of the tensile equipment as needed, turn on the tensile test equipment, apply an external force to gradually stretch the locust intersegmental membrane specimens. When the specimens break, the tensile equipment stops stretching, and organize and calculate the tensile data. Fourth step, conduct puncture detection on the ovipositor of the locust through a texture analyzer: When the locust molts, regularly collect its ovipositors at different time points as samples; fix the ovipositor on the specimen slide (11) through an adhesive, and install the probe (13) on the extension rod (12). Fifth step, start the texture analyzer to make the extension rod (12) puncture the ovipositor. The texture analyzer records the change in the puncture force value of the extension rod (12). When the probe reaches the puncture distance or the maximum force value, the texture analyzer automatically stops measuring, and repeat the measurement for multiple samples at each time point.

Images