Feed additive and feed for improving poultry egg quality and application thereof

Abstract

The present invention provides a feed additive and feed for improving the quality of poultry eggs, and their application, and belongs to the field of feed technology. The present invention provides a feed additive, which comprises, by mass, 20 to 30 parts of lycopene, 10 to 20 parts of capsanthin, 20 to 30 parts of pumpkin powder, and 20 to 30 parts of zeaxanthin. Lycopene and zeaxanthin have antioxidant and immunity-enhancing effects; capsanthin has antioxidant, anti-inflammatory, immunity-enhancing and anti-cancer effects; pumpkin powder has blood sugar regulation, digestion promotion, anti-aging, and immunity-enhancing effects. The feed additive provided by the present invention uses lycopene, capsanthin, pumpkin powder, and zeaxanthin in combination, which can synergistically increase the egg production of laying poultry, improve egg quality, and increase the nutritional value of eggs.

Description

Technical Field

[0001] The invention belongs to the technical field of feed, and particularly relates to a feed additive and feed for improving the quality of poultry eggs, and applications thereof. Background Art

[0002] With the continuous development of the national economy and the continuous improvement of residents' living standards, coupled with increased health awareness, consumption upgrades, and greater attention to food safety, the demand for and consumption of high-quality livestock and poultry products is on the rise. Eggs are one of the most affordable and nutritious foods in our daily lives. As society develops, the demand for eggs must be met not only in quantity but also in quality. Green, healthy, safe, and functional eggs have become a major consumer trend.

[0003] In large-scale laying hen farming, laying hens are susceptible to various internal and external factors, leading to stress, which can severely impact their production performance and egg quality, and can even cause death, resulting in significant economic losses. For example, during peak egg production, laying hens are susceptible to yolk peritonitis due to external factors. Furthermore, excessive egg production during peak egg production can lead to oviduct prolapse and egg-laying difficulties, further impacting egg production and / or quality.

[0004] In summary, while the public demand for high-quality livestock and poultry products is increasing, large-scale egg-laying chicken production is susceptible to internal and external factors, leading to economic losses. Therefore, producing high-quality eggs to meet public demand while reducing economic losses in the livestock industry is crucial. Furthermore, the issue of antibiotic residues in poultry is becoming increasingly prominent worldwide. To address the current problem of drug residues in egg products, developing an effective feed additive that can replace antibiotics has become a key research topic for industry professionals. Summary of the Invention

[0005] In view of the defects in the prior art, the object of the present invention is to provide a feed additive that does not contain antibiotics and can simultaneously improve the yield and / or quality of eggs.

[0006] The purpose of the present invention is achieved through the following technical solutions:

[0007] The invention provides a feed additive comprising lycopene, capsanthin, pumpkin powder and zeaxanthin.

[0008] Preferably, the feed additive comprises, by weight:

[0009] 20-30 parts of lycopene, 10-20 parts of capsanthin, 20-30 parts of pumpkin powder and 20-30 parts of lutein.

[0010] The present invention provides a method for preparing the feed additive described in the above technical solution, comprising:

[0011] Lycopene, capsanthin, pumpkin powder and zeaxanthin are mixed to obtain a feed additive.

[0012] The present invention provides a feed comprising the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution.

[0013] Preferably, the feed further comprises a basal diet.

[0014] Preferably, the amount of feed additive added to the basic diet is 0.1wt.%~0.5wt.%.

[0015] The present invention provides the feed additive described in the above technical solution, the feed additive prepared by the preparation method described in the above technical solution, or the feed described in the above technical solution for use in egg-laying poultry breeding.

[0016] Preferably, the application includes any one or two or more of the following (1) to (4):

[0017] (1) Increase the average daily feed intake of poultry;

[0018] (2) Increase egg production in poultry;

[0019] (3) Improve egg quality;

[0020] (4) Improve the nutritional value of eggs.

[0021] Preferably, the poultry comprises chicken.

[0022] The present invention provides a method for increasing poultry egg production and / or improving egg quality, comprising:

[0023] The poultry is fed with feed containing the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution.

[0024] Beneficial effects of the present invention:

[0025] The present invention provides a feed additive comprising: lycopene, capsanthin, pumpkin powder, and zeaxanthin. In the present invention, lycopene and zeaxanthin have antioxidant and immunity-enhancing effects; capsanthin has antioxidant, anti-inflammatory, immunity-enhancing, and anti-cancer effects; and pumpkin powder has blood sugar regulation, digestion promotion, anti-aging, and immunity-enhancing effects. The feed additive provided by the present invention uses lycopene, capsanthin, pumpkin powder, and zeaxanthin in combination, which can synergistically increase egg production, improve egg quality, and enhance the nutritional value of laying poultry. Furthermore, the raw materials in the feed additive are all plant extracts, which are safe and have no toxic side effects. The production cost is low, and the feed additive does not contain antibiotics. The present invention demonstrates through examples that the feed additive can significantly increase the average daily feed intake of laying hens and increase weekly egg production; the feed additive can significantly improve the yolk color score, albumen height, and Haugh unit of eggs, thereby improving egg quality; the feed additive can significantly increase the content of vitamin A and polyunsaturated fatty acids in eggs, and significantly increase the content of antioxidants in eggs, thereby improving the nutritional value of eggs. In summary, the feed additive provided by the present invention is beneficial for improving egg production and / or quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0027] Figure 1 This is the yolk color diagram of eggs in each experimental group after one week of the experiment;

[0028] Figure 2 This is the yolk color chart of eggs in each experimental group during the second week of the experiment;

[0029] Figure 3 This is the yolk color chart of eggs in each experimental group during the 3-week experiment;

[0030] Figure 4 This is a diagram of the yolk color of eggs in each experimental group during the 4th week of the experiment;

[0031] Figure 5 This is a diagram of the yolk color of eggs in each experimental group during the 5-week experiment;

[0032] Figure 6 This is a diagram of the yolk color of eggs in each experimental group during the 6-week experiment;

[0033] Figure 7 This is a diagram of the yolk color of eggs in each experimental group during the 7th week of the experiment;

[0034] Figure 8 This is a color chart for scoring the yolk color of eggs in each experimental group during the 7th week of the experiment;

[0035] Figure 9 This is a comparison chart of the yolk colors of eggs in each experimental group from week 1 to week 7 of the experiment. DETAILED DESCRIPTION

[0036] The invention provides a feed additive comprising lycopene, capsanthin, pumpkin powder and zeaxanthin.

[0037] In the following technical solutions, the present invention has no special limitation on the sources of the raw materials, and any conventional commercially available products in the art may be used.

[0038] As an optional embodiment of the present invention, the mass content of lycopene in the feed additive can be 20-30 parts by mass, or can be 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30 parts by mass.

[0039] As an optional embodiment of the present invention, the mass portion of capsanthin in the feed additive can be 10-20 parts by mass of lycopene, and can be 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 parts.

[0040] As an optional embodiment of the present invention, the mass parts of pumpkin powder in the feed additive can be 20-30 parts, or 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30 parts, calculated based on the mass parts of lycopene.

[0041] As an optional embodiment of the present invention, the mass portion of lutein in the feed additive can be 20-30 parts by mass, or can be 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30 parts by mass, calculated based on the mass portion of lycopene.

[0042] In the feed additive provided by the present invention, lycopene and zeaxanthin have antioxidant and immune-enhancing effects; capsanthin has antioxidant, anti-inflammatory, immune-enhancing, and anti-cancer effects; and pumpkin powder has blood sugar regulation, digestion promotion, anti-aging, and immune-enhancing effects. The combined use of lycopene, capsanthin, pumpkin powder, and zeaxanthin in the feed additive provided by the present invention can synergistically increase egg production, improve egg quality, and enhance the nutritional value of laying poultry.

[0043] The present invention provides a method for preparing the feed additive described in the above technical solution, comprising: mixing lycopene, capsanthin, pumpkin powder, and zeaxanthin to obtain the feed additive. The present invention is not particularly limited to the mixing method, and conventional mixing methods in the art can be used to uniformly mix the ingredients.

[0044] The present invention provides a feed comprising the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution. As an optional embodiment of the present invention, the feed further comprises a basal diet. The present invention has no special restrictions on the composition of the basal diet, and any basal diet for conventional poultry feeding in the field can be used. As an optional embodiment of the present invention, the amount of the feed additive added to the basal diet is 0.1wt.%~0.5wt.%, and can be 0.1wt.%, 0.2wt.%, 0.3wt.%, 0.4wt.% or 0.5wt.%.

[0045] In the present invention, the method for preparing the feed comprises: uniformly mixing the feed additive with other components.

[0046] The present invention provides the use of the feed additive described in the above technical solution, the feed additive prepared by the preparation method described in the above technical solution, or the feed described in the above technical solution in egg-laying poultry farming. As an optional embodiment of the present invention, the poultry includes chickens. In the present invention, the use includes any one or more of the following (1) to (4):

[0047] (1) Increase the average daily feed intake of poultry;

[0048] (2) Increase egg production in poultry;

[0049] (3) Improve egg quality;

[0050] (4) Improve the nutritional value of eggs.

[0051] The feed additive or feed provided by the present invention can increase the average daily feed intake of poultry. The present invention shows through the results of examples that the feed additive can significantly increase the average daily feed intake of laying hens.

[0052] The feed additive or feed provided by the present invention can increase the egg production of poultry. The present invention shows through the results of the examples that the feed additive can significantly increase the weekly egg production of laying hens.

[0053] The feed additive or feed provided by the present invention can improve the quality of eggs. As an optional embodiment of the present invention, the improvement of egg quality includes improving any two or more of the following: egg weight, yolk weight, egg white weight, egg shell weight, yolk color score, egg white height, egg shell thickness, and Haugh unit. The present invention shows through the results of the examples that the feed additive is beneficial to improving the egg weight, yolk weight, egg white weight, egg shell weight, yolk color score, egg white height, egg shell thickness, and Haugh unit of eggs, and can significantly improve the yolk score, egg white height, and Haugh unit, thereby improving the quality of eggs.

[0054] The feed additive or feed provided by the present invention can improve the nutritional value of eggs. As an optional embodiment of the present invention, improving the nutritional value of eggs includes increasing any one or more of vitamin A, polyunsaturated fatty acids, and antioxidants in eggs. The results of the present invention, through examples, demonstrate that the feed additive can significantly increase the content of vitamin A and polyunsaturated fatty acids in eggs, significantly increase the content of antioxidants in eggs, and thus contribute to improving the nutritional value of eggs.

[0055] The present invention provides a method for increasing poultry egg production and / or improving egg quality, comprising:

[0056] The poultry is fed with feed containing the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution.

[0057] As an optional embodiment of the present invention, the present invention can add the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution to the basic diet for feeding poultry. In the embodiment of the present invention, the effect of the feed additive is verified by taking chicken as an example.

[0058] As an optional embodiment of the present invention, the addition amount of the feed additive in the basic diet can be 0.1wt.%~0.5wt.%, and can be 0.1wt.%, 0.2wt.%, 0.3wt.%, 0.4wt.% or 0.5wt.%.

[0059] In order to further illustrate the present invention, the technical solution provided by the present invention is described in detail below with reference to the accompanying drawings and embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0060] Lycopene and capsanthin used in the following examples were purchased from Chenguang Biotechnology Group Handan Co., Ltd., and pumpkin powder and zeaxanthin were purchased from Henan Ren'an Biotechnology Co., Ltd.

[0061] Example 1

[0062] A feed additive comprises, by weight, 20 parts of lycopene, 10 parts of capsanthin, 20 parts of pumpkin powder and 20 parts of zeaxanthin.

[0063] The preparation method of the feed additive is:

[0064] Lycopene, capsanthin, pumpkin powder and zeaxanthin are mixed according to parts by mass to obtain a feed additive.

[0065] Example 2

[0066] A feed additive comprises, by weight, 30 parts of lycopene, 20 parts of capsanthin, 30 parts of pumpkin powder and 30 parts of zeaxanthin.

[0067] The preparation method of the feed additive is the same as that in Example 1.

[0068] Example 3

[0069] A feed additive comprises, by weight, 25 parts of lycopene, 15 parts of capsanthin, 25 parts of pumpkin powder and 25 parts of zeaxanthin.

[0070] The preparation method of the feed additive is the same as that in Example 1.

[0071] Example 4

[0072] A feed comprising: a basic diet and the feed additive in Example 1, wherein the amount of the feed additive added to the basic diet is 0.15 wt.%.

[0073] The basic diet was purchased from Changchun Hefeng Feed Co., Ltd., and its composition is shown in Table 1.

[0074] Table 1 Composition of basal diet

[0075]

[0076] Note: 1. The premix content per kilogram of diet is as follows: Vitamin A 8000 IU, Vitamin D 3750 IU, Vitamin E 100 mg, Vitamin B2 12.5 mg, Vitamin B6 9 mg, Vitamin B12 0.03 mg, Pantothenic acid 18 mg, Niacin 60 mg, Folic acid 1.5 mg, Biotin 0.225 mg, Iron 80 mg, Copper 9 mg, Selenium 0.3 mg, Manganese 12.25 mg, and Zinc 25.2 mg. 2. The values ​​in the table are calculated based on data provided by the China Feed Database (2013).

[0077] The method for preparing the feed is as follows: uniformly mixing the basic diet and the feed additive to obtain the feed.

[0078] Example 5

[0079] A feed comprising: a basic diet and the feed additive of Example 1, wherein the amount of the feed additive added to the basic diet is 0.25 wt.%. The composition of the basic diet is the same as that of Example 4.

[0080] Example 6

[0081] A feed comprising: a basic diet and the feed additive of Example 1, wherein the amount of the feed additive added to the basic diet is 0.4 wt.%. The composition of the basic diet is the same as that of Example 4.

[0082] Example 7

[0083] A feed comprising: a basic diet and the feed additive of Example 1, wherein the amount of the feed additive added to the basic diet is 0.5 wt.%. The composition of the basic diet is the same as that of Example 4.

[0084] Example 8

[0085] A feed comprising: a basic diet and the feed additive of Example 2, wherein the amount of the feed additive added to the basic diet is 0.2 wt.%. The composition of the basic diet is the same as that of Example 4.

[0086] Example 9

[0087] A feed comprising: a basic diet and the feed additive of Example 2, wherein the amount of the feed additive added to the basic diet is 0.3 wt.%. The composition of the basic diet is the same as that of Example 4.

[0088] Example 10

[0089] A feed comprising: a basic diet and the feed additive of Example 2, wherein the amount of the feed additive added to the basic diet is 0.5 wt.%. The composition of the basic diet is the same as that of Example 4.

[0090] Example 11

[0091] A feed comprising: a basic diet and the feed additive of Example 3, wherein the amount of the feed additive added to the basic diet is 0.1 wt.%. The composition of the basic diet is the same as that of Example 4.

[0092] Example 12

[0093] A feed comprising: a basic diet and the feed additive of Example 3, wherein the amount of the feed additive added to the basic diet is 0.3 wt.%. The composition of the basic diet is the same as that of Example 4.

[0094] Example 13

[0095] A feed comprising: a basic diet and the feed additive of Example 3, wherein the amount of the feed additive added to the basic diet is 0.5 wt.%. The composition of the basic diet is the same as that of Example 4.

[0096] Application Example 1

[0097] Verification of the feeding effect of feed containing feed additives on laying hens

[0098] 1. Animal Selection and Experimental Design

[0099] A total of 360 185-day-old Beijing-style laying hens were selected and randomly divided into 4 groups, with 90 chickens in each group; they were named blank group, experimental group 1, experimental group 2 and experimental group 3, and each group was randomly divided into 3 small groups, with 30 chickens in each group; the pre-test lasted 3 days, and the feed intake, egg production and health status of the laying hens were observed.

[0100] Among them, each chicken in the blank group was fed a basic diet every day, experimental group 1 was fed with feed supplemented with 0.15% feed additives in the basic diet, experimental group 2 was fed with feed supplemented with 0.25% feed additives in the basic diet, and experimental group 3 was fed with feed supplemented with 0.4% feed additives in the basic diet. Each experimental group was fed three times a day, morning, noon and evening, with each chicken fed 50g each time; the amount of leftover feed and egg production were recorded every morning, and the average daily feed intake (g) of each laying hen in each group and the weekly egg production (pieces) of each experimental group (30 laying hens) were calculated. The egg weight, yolk weight, albumen weight, albumen height, eggshell thickness, eggshell weight of the eggs produced by the laying hens in each experimental group were tested every 7 days, and the yolk color score was recorded according to the Roche colorimetric fan. The pictures of the yolk color in the eggs from the 1st to 7th week of the experiment are as follows: Figures 1 to 9 On the 49th day of the experiment, 100 eggs were collected from each group for testing of egg nutritional components and antioxidant indicators in egg yolk.

[0101] The basal diet is the basal diet in Example 4, and the feed additive is the feed additive in Example 1.

[0102] 2. Layer breeding and management

[0103] In accordance with the Jinghuadu Group's production management manual, we adopt a large-scale production method, with stepped cages, 3 birds per cage, natural ventilation, free drinking water, keeping the pens clean and hygienic, and carrying out the immunization program as usual.

[0104] 3. Slaughter and sample collection

[0105] On the 49th day of the experiment, 100 eggs were collected from each experimental group to detect the content of polyunsaturated fatty acids and vitamin A in the eggs, as well as the antioxidant indicators of SOD, T-AOC, DPPH and reducing power in the egg yolk.

[0106] 4. Experimental data collection and result analysis

[0107] The experimental data were preliminarily sorted and counted using Excel, and then variance analysis was performed using SPSS (29.0) statistical software. The least significant difference method was used for multiple comparisons; P < 0.05 and P < 0.01 were used as the criteria for judging significant and extremely significant differences.

[0108] 5. Test results

[0109] (1) The test results of laying hen production performance indicators are shown in Table 2.

[0110] Table 2 Test results of laying hen production performance indicators

[0111]

[0112] Note: The superscript letters on the right side of the data in the table indicate significant differences between the groups (P<0.05) and are marked according to statistical significance.

[0113] As shown in Table 2, there was no significant difference in the average daily feed intake, weekly egg production and feed-to-egg ratio of experimental group 1, experimental group 2 and experimental group 3 in the first two weeks of the experiment. In the third week of the experiment, the average daily feed intake of experimental group 1 was significantly increased relative to the blank group, and the weekly egg production of experimental groups 1 and 3 was significantly increased relative to the blank group. In the fourth week of the experiment, the average daily feed intake of experimental group 2 was significantly increased relative to the blank group, and the weekly egg production of experimental groups 1, 2 and 3 was significantly increased relative to the blank group. In the fifth week of the experiment, the weekly egg production of experimental groups 1 and 3 was significantly increased relative to the blank group. In the sixth week of the experiment, the average daily feed intake of experimental group 1 was significantly increased, and the weekly egg production of experimental groups 1 and 3 was significantly increased relative to the blank group. In the seventh week of the experiment, the average daily feed intake of experimental groups 1 and 3 was significantly increased relative to the blank group, and the weekly egg production of experimental groups 1, 2 and 3 was significantly increased relative to the blank group. The results show that adding the feed additive provided by the present invention to the basic diet of laying hens can effectively increase egg production and daily feed intake.

[0114] (2) The test results of egg quality indicators are shown in Table 3.

[0115] Table 3 Test results of egg quality indicators

[0116]

[0117]

[0118] Note: The lowercase letters on the right side of the data in the table indicate significant differences between the groups (P<0.05), and the capital letters indicate extremely significant differences between the groups (P<0.01), and are marked according to statistical significance.

[0119] As shown in Table 3, in the first week of the experiment, the yolk color scores of experimental groups 1, 2, and 3 were significantly improved compared with the blank control group (P<0.01), the protein height of experimental group 1 was significantly improved compared with the blank group (P<0.05), and the Haugh unit of experimental group 3 was significantly improved compared with the blank control group (P<0.05); in the second week of the experiment, the egg weight and protein weight of experimental group 1 were significantly increased compared with the blank control group (P<0.05), the eggshell weight of experimental groups 1 and 3 were significantly increased compared with the blank control group (P<0.05), the yolk color scores of experimental groups 1, 2, and 3 were significantly improved compared with the blank control group (P<0.01), the protein height of experimental group 1 was significantly improved compared with the blank group (P<0.05), the Haugh unit of the experimental group 1 was significantly increased compared with the blank group (P<0.05); in the third week of the experiment, the egg weight of the experimental groups 1 and 3 was significantly increased compared with the blank group (P<0.05), the yolk weight of the experimental group 1 was significantly increased compared with the blank group (P<0.05), the albumen weight of the experimental groups 1 and 3 was significantly increased compared with the blank group (P<0.05), the yolk score of the experimental groups 1, 2 and 3 was significantly increased compared with the blank group (P<0.01), the albumen height of the experimental group 1 was significantly increased compared with the blank group (P<0.05), and the eggshell thickness of the experimental group 3 was significantly increased compared with the blank control group (P<0.05); in the fourth week of the experiment, the egg weight of the experimental group 1 was significantly increased compared with the blank group (P<0.05). The results showed that the egg yolk weight of experimental group 3 was significantly increased compared with the blank control group (P<0.05), the egg white weight of experimental groups 1 and 3 was significantly increased compared with the blank group (P<0.05), the egg yolk color score of experimental groups 1, 2 and 3 was significantly increased compared with the blank control group (P<0.01), and the egg shell thickness of experimental group 3 was significantly increased compared with the blank group (P<0.05); in the 5th week of the experiment, the egg shell weight of experimental groups 1 and 3 was significantly increased compared with the blank group (P<0.05), and the egg yolk color score and egg shell thickness of experimental groups 1, 2 and 3 were significantly increased compared with the blank group (P<0.01); in the 6th week of the experiment, the egg yolk weight of experimental groups 1, 2 ...1). Egg weight, yolk weight, yolk color score, albumen height and Haugh unit were significantly increased compared with the blank group (P<0.05), the albumen weight of experimental group 2 was significantly increased compared with the blank group (P<0.05), and the eggshell thickness of experimental group 3 was significantly increased compared with the blank group (P<0.05). In the 7th week of the experiment, the yolk color score, albumen height, eggshell thickness and Haugh unit of experimental groups 1, 2 and 3 were significantly increased compared with the blank group (P<0.05), the egg weight of experimental groups 1 and 3 was significantly increased compared with the blank group, the albumen weight of experimental groups 1 and 2 was significantly increased compared with the blank group (P<0.05), and the yolk weight of experimental group 2 was significantly increased compared with the blank group (P<0.05).

[0120] In summary, the feed additive provided by the present invention can significantly improve the yolk color score once fed, and the improvement effect is stable; as the feeding time is extended, the feed additive can significantly and stably increase the protein height and Haugh unit by the 6th to 7th week of feeding, thereby improving the quality of eggs.

[0121] (3) The test results of the nutritional indexes in eggs are shown in Table 4.

[0122] Table 4 Test results of nutritional components in eggs

[0123]

[0124] As can be seen from Table 4, the vitamin A and polyunsaturated fatty acid contents in the eggs of Experimental Group 1 were significantly increased compared to those in the blank group (P < 0.01); the polyunsaturated fatty acid content in the eggs of Experimental Group 2 was significantly increased compared to the blank group (P < 0.01); and the vitamin A and polyunsaturated fatty acid content in the eggs of Experimental Group 3 was significantly increased compared to the blank group (P < 0.01). These results demonstrate that the addition of the feed additive provided by the present invention to the basal diet of laying hens can effectively increase the vitamin A and polyunsaturated fatty acid content in eggs, thereby increasing the nutrient content of the eggs.

[0125] (4) The results of the antioxidant test in eggs are shown in Table 5.

[0126] Table 5 Antioxidant test results in eggs

[0127]

[0128] Superoxide dismutase (SOD) is an important antioxidant enzyme that scavenges reactive oxygen free radicals and protects cells from oxidative stress. A high SOD content in eggs can increase their nutritional value, and consuming eggs with high SOD activity can improve the body's antioxidant capacity. The diphenyl picrohydrazyl (DPPH) free radical scavenging capacity reflects the antioxidant capacity of eggs. A higher DPPH scavenging rate is associated with yolk color, freshness, and nutritional value. Total antioxidant capacity (T-AOC) is an important indicator of the overall antioxidant properties of eggs. Higher T-AOC levels indicate greater antioxidant capacity. T-AOC levels are positively correlated with nutritional value, shelf life, and yellowish color. Testing the reducing power of egg yolks shows a positive correlation with their antioxidant capacity and potential to resist oxidative damage. Higher reducing power indicates fresher eggs, higher nutritional value, and brighter yolk color.

[0129] As can be seen from Table 5, the reducing power, DPPH, SOD, and T-AOC contents in the egg yolks of Experimental Groups 1, 2, and 3 were significantly higher than those in the blank group (P<0.01). The results show that the addition of the feed additive composition can significantly increase the reducing power, superoxide dismutase (SOD), diphenylpicrylhydrazyl free radical (DPPH), and total antioxidant capacity (T-AOC) contents in egg yolks. Adding the feed additive provided by the present invention to the basic diet of laying hens can effectively enhance the antioxidant capacity of eggs.

[0130] Comparative Example 1

[0131] A feed additive consisting solely of lycopene.

[0132] A feed consists of a basic diet and lycopene, wherein the added amount of lycopene in the basic diet is 0.15%.

[0133] The composition of the basal diet was the same as in Example 4.

[0134] Comparative Example 2

[0135] A feed additive consists of lycopene, capsanthin and pumpkin powder, wherein the mass ratio of lycopene, capsanthin and pumpkin powder is 3:2:3.

[0136] A feed comprises a basic diet and the feed additive in this comparative example, wherein the addition amount of the feed additive in the basic diet is 0.15%.

[0137] The composition of the basal diet was the same as in Example 4.

[0138] Comparative Example 3

[0139] A feed additive comprises capsanthin, pumpkin powder, and zeaxanthin, wherein the mass ratio of capsanthin, pumpkin powder, and zeaxanthin is 3:2:3. The amount of the feed additive added to the basic diet is 0.15%.

[0140] The composition of the basal diet was the same as in Example 4.

[0141] Application Example 2

[0142] Feeding effect of the feed provided by Comparative Example 1, Comparative Example 2 and Comparative Example 3

[0143] The experiment in Application Example 2 was conducted at the same time as the experiment in Application Example 1, and the animals selected were the same batch of 185-day-old Beijing-red laying hens. The feeding method and laying hen breeding and management methods were exactly the same. To facilitate the recording of the results, they are listed in the form of Application Example 2.

[0144] 1. Animal Selection and Experimental Design

[0145] 360 185-day-old Beijing-style laying hens were randomly divided into four groups of 90 each, designated as blank, comparative example 1, comparative example 2, and comparative example 3. Each group was randomly divided into three subgroups of 30 each. Over a three-day pre-test, the feed intake, egg production, and health of the laying hens were observed. The composition of the basal diet was the same as that in Example 4.

[0146] The blank group was fed a basal diet daily, control group 1 was fed the feed of control group 1, control group 2 was fed the feed of control group 2, and control group 3 was fed the feed of control group 3. The experimental groups were fed the same feed or the blank group's basal diet three times a day, morning, noon, and evening. Feed residue and egg production were recorded every morning to calculate the average daily feed intake (g) per laying hen and the weekly egg production (eggs) for each experimental group (30 laying hens). Egg weight, yolk weight, albumen weight, albumen height, shell thickness, and shell weight were measured every seven days. On day 42 of the experiment, 100 eggs from each group were collected for testing of egg nutrients and antioxidant indicators in the yolk.

[0147] 2. Layer breeding and management

[0148] In accordance with the Jinghuadu Group's production management manual, we adopt a large-scale production method, with stepped cages, 3 birds per cage, natural ventilation, free drinking water, keeping the pens clean and hygienic, and carrying out the immunization program as usual.

[0149] 3. Slaughter and sample collection

[0150] On the 49th day of the experiment, 100 eggs were collected from each experimental group to detect the content of polyunsaturated fatty acids and vitamin A in the eggs, as well as the antioxidant indicators of SOD, T-AOC, DPPH and reducing power in the egg yolk.

[0151] 4. Experimental data collection and result analysis

[0152] The experimental data were preliminarily sorted and counted using Excel, and then variance analysis was performed using SPSS (29.0) statistical software. The least significant difference method was used for multiple comparisons; P < 0.05 and P < 0.01 were used as the criteria for judging significant and extremely significant differences.

[0153] 5. Test results

[0154] (1) The test results of laying hen production performance are shown in Table 7.

[0155] Table 7 Results of production performance test of laying hens

[0156]

[0157] As shown in Table 7, on day 21 of the experiment, the average daily feed intake of control groups 2 and 3 was significantly higher than that of the blank group and control group 1 (P < 0.05). On day 28 of the experiment, the average daily feed intake of control group 2 was significantly higher than that of the blank group (P < 0.05). On day 35 of the experiment, the weekly egg production of control group 2 was significantly higher than that of the blank group (P < 0.05).

[0158] (2) The test results of the nutritional indexes in eggs are shown in Table 8.

[0159] Table 8 Test results of nutritional components in eggs

[0160]

[0161] As shown in Table 8, the vitamin A content in Comparative Example 2 was significantly increased compared to the blank group (P < 0.01), and the polyunsaturated fatty acid content in Comparative Example Groups 1 and 3 was significantly increased compared to the blank group (P < 0.01). Adding lycopene to the basal diet significantly increased the vitamin A content in eggs compared to adding the basal diet alone. The group fed a feed additive containing lycopene, capsanthin, and pumpkin powder did not produce significant changes in improving vitamin A content in eggs compared to the blank group. Adding lycopene or a feed additive consisting of lycopene, capsanthin, and pumpkin powder to the basal diet significantly increased the polyunsaturated fatty acid content in eggs compared to the blank group.

[0162] (3) The results of the antioxidant test in eggs are shown in Table 9.

[0163] Table 9 Antioxidant test results in eggs

[0164]

[0165] As shown in Table 9, compared with the blank group, the reducing power of Comparative Example 3 group was significantly improved (P<0.05), the DPPH content of Comparative Example 1, 2, and 3 groups was significantly increased (P<0.01), and the SOD and T-AOC content of Comparative Example 1 group was significantly increased (P<0.05). The T-AOC content of Comparative Example 1, 2, and 3 groups was significantly increased (P<0.01).

[0166] (4) The egg quality test results are shown in Tables 10 to 15.

[0167] Table 10 Egg quality test results of each experimental group in the first week

[0168]

[0169] Table 11 Egg quality test results of each experimental group in the second week

[0170]

[0171] Table 12 Egg quality test results of each experimental group in the third week

[0172]

[0173] Table 13 Egg quality test results of each experimental group in the fourth week

[0174]

[0175] Table 14 Egg quality test results of each experimental group in the fifth week

[0176]

[0177] Table 15 Egg quality test results of each experimental group in the sixth week

[0178]

[0179] In summary, the feed additive provided by the present invention can significantly increase the average daily feed intake of laying hens, increase egg production, improve egg quality, and improve the nutritional value of eggs.

[0180] Although the above embodiment provides a detailed description of the present invention, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A feed additive or feed for improving egg quality, wherein the feed additive comprises, by weight, 20-30 parts of lycopene, 10-20 parts of capsanthin, 20-30 parts of pumpkin powder, and 20-30 parts of zeaxanthin. The feed comprises a basic diet and the feed additive; The amount of the feed additive added to the basic diet is 0.4wt.%; The improving egg quality is improving eggshell thickness and / or eggshell weight.

2. The application according to claim 1, characterized in that The preparation method of the feed additive comprises: Lycopene, capsanthin, pumpkin powder and zeaxanthin are mixed to obtain a feed additive.

3. The application according to claim 1, characterized in that The improvement of egg quality also includes: improving any one or more of albumen height, yolk weight, albumen weight, Haugh units, vitamin A, polyunsaturated fatty acids and antioxidants.

4. A method for improving eggshell weight and / or eggshell thickness, characterized in that: include: Feeding poultry with a feed containing the feed additive according to claim 1; The poultry is chicken; The addition amount of the feed additive in the basic diet is 0.4wt.%.

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