Liquid cover film

Abstract

The invention relates to a liquid cover film which comprises a tail plate, a cover plate and a connecting plate, the tail plate and the connecting plate are arranged at the two ends of the cover plate respectively, a reaction cavity is formed between the cover plate and a glass slide, and a drainage plate is arranged in the middle of the tail plate; the first end face is arranged on the lower end face of the tail plate and connected with the end, facing the cover plate, of the drainage plate. The second end face is adjacent to the first end face; the liquid homogenizing bulges are arranged on the second end surface, and the number of the liquid homogenizing bulges is multiple; the multiple drainage grooves are formed in the first end face and formed in the two sides of the drainage plate. Due to the fact that the drainage groove is formed in the first end face of the tail plate, in the reaction liquid adding stage, reaction liquid can rapidly flow into the reaction cavity along the drainage groove, and rapid filling of the reaction cavity is promoted; and the second end face is also provided with a liquid homogenizing bulge, so that the liquid homogenizing bulge can uniformly distribute the reaction liquid on the glass slide when the liquid cover film is dragged by the full-automatic immunohistochemical instrument, and the uniform mixing of the reaction liquid is accelerated.

Description

Technical Field

[0001] The present invention relates to the technical field of biological sample slide processing, and particularly to a liquid cover film. Background Art

[0002] During the process of processing a biological sample slide, a reaction solution needs to be added into the reaction chamber between the liquid cover film and the glass slide, so that the biological sample and the reaction solution fully react in the reaction chamber, and quantitative or qualitative analysis is performed on antigen polypeptides and proteins in the biological sample tissue section.

[0003] Currently, in the prior art, the fitting plane of the liquid cover film and the glass slide often forms a certain inclination angle. Then, the reaction solution is added at one end, and by using the gravity of the fluid, the reaction solution enters the reaction chamber, and the gas in the reaction chamber is expelled, so that the reaction chamber is filled with the reaction solution. Chinese Patent with Publication No. CN111562164A discloses a liquid cover film. A drainage column for introducing the reaction solution onto the liquid distribution part is provided in the middle of the front end of the liquid cover film body. When adding the reaction solution, the reaction solution is added onto the drainage column to ensure that the liquid is added to the middle position at the level of the glass slide. Due to the special structure of the conical part, the liquid will uniformly diffuse from the middle downwards, quickly fill the liquid distribution part, and then the reaction solution will uniformly and quickly diffuse with the same width as the reaction chamber throughout the reaction chamber, so as to uniformly cover the biological sample, promoting the full reaction of the biological sample and the reaction solution. However, the reaction solution can only flow into the reaction chamber along the drainage column, which results in that the amount of the reaction solution added instantaneously cannot be too large. It is necessary to ensure that the reaction solution only flows downward along the drainage column, thereby increasing the time for adding the reaction solution, and it is also impossible to reduce the time for the reaction solution to uniformly diffuse into the reaction chamber. The reaction solution flowing into the reaction chamber still diffuses in the reaction chamber by relying on gravity. In addition, if the reaction solution in the reaction chamber is stirred too much, it is easy to damage the tissue section in the reaction solution.

[0004] Therefore, the present invention provides a liquid cover film. Summary of the Invention

[0005] In view of the above-mentioned disadvantages and deficiencies of the prior art, the present invention provides a liquid cover film, thereby solving the technical problem of how to quickly and uniformly diffuse the reaction solution in the reaction chamber.

[0006] To achieve the above object, the present invention provides a liquid cover film, including a tail plate, a cover plate and a connecting plate, and the tail plate and the connecting plate are respectively arranged at both ends of the cover plate. A reaction chamber is formed between the cover plate and the glass slide. The liquid cover film further includes: a drainage plate, which is arranged in the middle of the tail plate; a first end face, which is arranged on the lower end face of the tail plate and is connected to the end of the drainage plate facing the cover plate; a second end face, which is arranged adjacent to the first end face; a plurality of liquid homogenizing protrusions, which are arranged on the second end face; a plurality of drainage grooves, which are opened on the first end face and are arranged on both sides of the drainage plate.

[0007] Optionally, the number of the liquid leveling protrusions is an even number, and the even number of liquid leveling protrusions are symmetrically arranged on both sides of the drainage plate.

[0008] Optionally, the even number of liquid leveling protrusions are evenly spaced on the second end face.

[0009] Optionally, the support columns are arranged on the tail plate, and there are two support columns, and the two support columns are symmetrically arranged on both sides of the drainage plate; in the horizontal direction, the extending length of the support columns is greater than that of the tail plate; in the horizontal direction, the extending length of the support columns is greater than that of the tail plate.

[0010] Optionally, the number of the drainage grooves is 2, and the 2 drainage grooves are symmetrically arranged on both sides of the drainage plate.

[0011] Optionally, along the extending direction of the drainage groove, from top to bottom, the opening degree of the drainage groove gradually increases.

[0012] Optionally, the extending direction of the drainage groove is towards the direction gradually away from the drainage plate.

[0013] Optionally, the height of the liquid leveling protrusion is 0.05 mm - 0.1 mm.

[0014] The beneficial effects of the present invention are as follows:

[0015] The present invention provides a liquid cover film. Since a plurality of drainage grooves are formed on the first end face of the tail plate, during the process of adding the reaction liquid to the reaction cavity, the reaction liquid can not only flow downward along the drainage plate, but also quickly flow into the reaction cavity in a diffused manner along the drainage grooves. Because the drainage grooves are located on both sides of the drainage plate, the distance between the reaction liquid flowing down along the drainage grooves and the edge of the reaction cavity is shorter, which is convenient for the reaction liquid to quickly fill the reaction cavity in the width direction of the reaction cavity; and by arranging a plurality of liquid leveling protrusions on the second end face of the tail plate, during the process of the liquid cover film being dragged by the fully automatic immunohistochemistry instrument, the plurality of liquid leveling protrusions provide a force for the reaction liquid to diffuse around the reaction cavity. On the one hand, as the liquid cover film is dragged, the plurality of liquid leveling protrusions accelerate the diffusion of the reaction liquid in the length direction of the reaction cavity. On the other hand, in the width direction of the reaction cavity, during the process of adding the reaction liquid, the plurality of liquid leveling protrusions will also occupy a certain volume of the reaction liquid, further promoting the diffusion of the reaction liquid in the width direction of the reaction cavity. In summary, by arranging a plurality of liquid leveling protrusions, the diffusion speed of the reaction liquid is accelerated, so that the reaction liquid in the reaction cavity is quickly and evenly distributed on the glass slide, and the mixing of the reaction liquid is accelerated. Description of the Drawings

[0016] Figure 1 is a schematic structural diagram of the liquid cover film of the present invention;

[0017] Figure 2 is Figure 1 the enlarged schematic diagram at A in

[0018] Description of Reference Numerals:

[0019] 1. Tail plate; 2. Cover plate; 3. Connecting plate; 4. First end face; 5. Second end face; 6. Liquid homogenizing protrusion; 7. Drainage plate; 8. Drainage groove; 9. Reaction chamber; 10. Support column. Detailed Description of the Invention

[0020] To better understand the above technical solution, the exemplary embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present invention are shown in the drawings, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided so that the present invention can be understood more clearly and thoroughly, and the scope of the present invention can be completely conveyed to those skilled in the art.

[0021] To facilitate the display of the structure on the side of the reaction chamber 9 in the liquid cover film of the present invention, Figure 1 and Figure 2 are schematic diagrams with the marked position of the reaction chamber 9 placed upwards. During the actual use of the liquid cover film, Figure 1 the marked position of the reaction chamber 9 is downwards.

[0022] An embodiment of the present invention provides a liquid cover film, as shown in Figure 1 and Figure 2 , which includes a tail plate 1, a cover plate 2 and a connecting plate 3, and the tail plate 1 and the connecting plate 3 are respectively arranged at both ends of the cover plate 2. A reaction chamber 9 is formed between the cover plate 2 and the glass slide. The liquid cover film further includes: a drainage plate 7 arranged in the middle of the tail plate 1; a first end face 4 arranged on the lower end face of the tail plate 1 and connected to the end of the drainage plate 7 facing the cover plate 2; a second end face 5 arranged adjacent to the first end face 4; a plurality of liquid homogenizing protrusions 6 arranged on the second end face 5; a plurality of drainage grooves 8 opened on the first end face 4 and arranged on both sides of the drainage plate 7.

[0023] Exemplarily, the liquid homogenizing protrusion 6 is integrally formed with the tail plate 1, and the shape of the liquid homogenizing protrusion 6 facing the glass slide is hemispherical, so as to ensure that when the liquid homogenizing protrusion 6 diffuses the reaction liquid in the reaction chamber 9, the hemispherical smooth surface faces the glass slide, avoiding damage to the tissue section in the reaction liquid during the liquid homogenizing process.

[0024] Exemplarily, the drainage plate 7 is a conical structure, which facilitates the reaction solution to flow rapidly along the cone towards the glass slide. Since the drainage plate 7 is arranged at the middle position of the tail plate 1, it is ensured that the position where most of the reaction solution flows into the reaction chamber 9 is at the middle in the width direction of the reaction chamber 9, which is convenient for the reaction solution to diffuse from the middle position of the glass slide to the surrounding. Since there is a certain distance between the middle position and the side of the reaction chamber 9, by arranging drainage grooves 8 on both sides of the drainage plate 7, during the process of adding the reaction solution, the extra part of the reaction solution can flow along the drainage grooves 8 towards the reaction chamber 9, thereby avoiding all the reaction solution flowing only along the conical drainage plate 7, accelerating the flow rate of the reaction solution towards the side of the reaction chamber 9, shortening the time for the reaction solution to fill the reaction chamber 9, and further improving the efficiency of the reaction solution filling the reaction chamber 9.

[0025] In a possible embodiment, as Figure 1 and Figure 2 shown, the number of the liquid homogenizing protrusions 6 is an even number, and the even number of liquid homogenizing protrusions 6 are symmetrically arranged on both sides of the drainage plate 7.

[0026] Exemplarily, the number of the liquid homogenizing protrusions 6 in this embodiment is 4, as Figure 2 shown. Since the drainage plate 7 is located at the middle position of the tail plate 1, correspondingly, 2 of the liquid homogenizing protrusions 6 are arranged on the left side of the drainage plate 7, and the other 2 liquid homogenizing protrusions 6 are arranged on the right side of the drainage plate 7. The connecting plate 3 at the end of the liquid cover film is connected to the power end of the automatic immunohistochemistry instrument. In this way, during the process of the liquid cover film being dragged by the automatic immunohistochemistry instrument, the 4 liquid homogenizing protrusions 6 provide a force for the reaction solution to diffuse towards the periphery of the reaction chamber 9, that is, 2 of the liquid homogenizing protrusions 6 accelerate the flow rate of the reaction solution near the left side of the reaction chamber 9, and the other 2 liquid homogenizing protrusions 6 accelerate the flow rate of the reaction solution near the right side of the reaction chamber 9. Thus, generally, the reaction solution in the reaction chamber 9 is evenly and rapidly distributed on the glass slide, accelerating the mixing of the reaction solution.

[0027] In a possible embodiment, as Figure 1 and Figure 2 shown, the even number of liquid homogenizing protrusions 6 are evenly spaced on the second end face 5.

[0028] Exemplarily, the number of the liquid homogenizing protrusions 6 in this embodiment is 2, and the 2 liquid homogenizing protrusions 6 are respectively opposite to the two drainage grooves 8, that is, the liquid homogenizing protrusions 6 on the same side of the drainage plate 7 correspond to the positions of the drainage grooves 8. Such a setting enables the reaction solution flowing in from the drainage grooves 8 to be more rapidly diffused through the corresponding liquid homogenizing protrusions 6, further accelerating the diffusion and mixing speed of the reaction solution.

[0029] Exemplarily, the number of the liquid homogenizing protrusions 6 in this embodiment is 4. The 4 liquid homogenizing protrusions 6 respectively face the edges of the two drainage grooves 8. Each drainage groove 8 has 2 edges in the flowing direction of the reaction liquid. By making the positions of the two liquid homogenizing protrusions 6 on the same side correspond to the two edges of the drainage groove 8, since the probability of the reaction liquid flowing along the edge of the drainage groove 8 is greater, such a setting enables the reaction liquid flowing in from the drainage groove 8 to be more quickly diffused through the corresponding liquid homogenizing protrusions 6, further accelerating the diffusion and mixing speed of the reaction liquid.

[0030] Exemplarily, the number of the liquid homogenizing protrusions 6 in this embodiment is 6. In the width direction of the reaction chamber 9, during the process of liquid homogenization by the liquid homogenizing protrusions 6, the 6 liquid homogenizing protrusions 6 can occupy a larger volume of the reaction liquid. Such a setting enables the reaction liquid flowing in from the drainage groove 8 to be more quickly diffused through the action of the liquid homogenizing protrusions 6, causing the diffusion of the reaction liquid in the width direction of the reaction chamber 9, and further accelerating the diffusion and mixing speed of the reaction liquid.

[0031] In a possible embodiment, as Figure 1 and Figure 2 shown, the support columns 10 are arranged on the tail plate 1, and there are two support columns 10, which are symmetrically arranged on both sides of the drainage plate 7; in the horizontal direction, the extending length of the support columns 10 is greater than that of the tail plate 1.

[0032] Exemplarily, before adding the reaction liquid to the reaction chamber 9, the connecting plate 3 of the liquid cover film is lifted to a certain height, so that the second end face 5 gradually approaches or abuts against the glass slide. At this time, if the connecting plate 3 is further lifted, the whole liquid cover film contacts the glass slide with the two support columns 10 as the support points, and at this time the tail plate 1 is in the middle position of the glass slide. Then the reaction liquid is added, and the main position for adding is the drainage plate 7. Since the drainage groove 8 is close to the drainage plate 7, part of the reaction liquid flows towards the glass slide in a diffused state along the drainage groove 8, thereby accelerating the uniform diffusion speed of the reaction liquid and accelerating the mixing of the reaction liquid.

[0033] Exemplarily, both the first end face 4 and the second end face 5 are strip-shaped end faces arranged between the two support columns 10, that is, both the first end face 4 and the second end face 5 are end faces arranged along the width direction of the reaction chamber 9. Among them, the first end face 4 is in contact with the drainage plate 7. Therefore, the drainage groove 8 is arranged on the first end face 4, facilitating the reaction liquid flowing down along the drainage plate 7 to flow towards the glass slide through the drainage groove 8.

[0034] In a possible embodiment, as Figure 1 and Figure 2 shown, the number of the drainage grooves 8 is 2, and the 2 drainage grooves 8 are symmetrically arranged on both sides of the drainage plate 7.

[0035] Exemplarily, if two drainage grooves 8 are symmetrically arranged, the diffusion degree of the reaction liquid flowing in through the two drainage grooves 8 is the same, which is conducive to the uniform diffusion of the reaction liquid after it enters the reaction chamber 9.

[0036] In a possible embodiment, as Figure 1 and Figure 2 shown, along the extending direction of the drainage groove 8, from top to bottom, the opening degree of the drainage groove 8 gradually increases.

[0037] Exemplarily, according to the placement direction of the liquid cover film in actual use, the opening degree above the drainage groove 8 is relatively small, while the opening degree below is relatively large. With such a setting, the reaction liquid flowing into the drainage groove 8 flows into the reaction chamber 9 in a diffused state, thereby increasing the diffusion speed of the reaction liquid in the reaction chamber 9.

[0038] In a possible embodiment, as Figure 1 and Figure 2 shown, the extending direction of the drainage groove 8 is in the direction gradually away from the drainage plate 7.

[0039] Exemplarily, the extending direction of the drainage groove 8 is inclined from the position close to the drainage plate 7 to the position away from the drainage plate 7, that is, gradually away from the drainage plate 7. With such a setting, it is conducive to the diffusion of the reaction liquid from the middle position of the reaction chamber 9 to the edges on both sides of the reaction chamber 9, thereby accelerating the diffusion speed of the reaction liquid in the reaction chamber 9.

[0040] In a possible embodiment, in the direction towards the reaction chamber 9, the height of the liquid - homogenizing protrusion 6 is 0.05 mm - 0.1 mm.

[0041] Exemplarily, the reaction chamber 9 is a cavity for accommodating the reaction liquid. In this embodiment, the height of the reaction chamber 9 itself is 2 mm. Therefore, in order to ensure sealing, the height of the liquid - homogenizing protrusion 6 in the vertical direction cannot be lower than the lower edge of the reaction chamber 9. The reaction chamber 9 is composed of a liquid cover film located above and a glass slide located below. Therefore, the four edges in the circumferential direction of the reaction chamber 9 are three edges of the cover plate 2 and the edge of the end plate 1. That is, the liquid - homogenizing protrusion 6 cannot be lower than the three edges of the cover plate 2 in the vertical direction, and at the same time, it cannot be lower than the edge of the end plate 1, ensuring that the three edges of the cover plate 2 and the edge of the end plate 1 can all be completely attached to the glass slide.

[0042] Exemplarily, in order to avoid the liquid - homogenizing protrusion 6 touching the tissue section and causing damage to the tissue section, the height of the liquid - homogenizing protrusion 6 should not be too high. In this embodiment, the height of the liquid - homogenizing protrusion 6 is 0.05 mm. Correspondingly, at this time, the concentration of the added reaction liquid is relatively small, the viscosity of the reaction liquid is also relatively small, and the fluidity of the reaction liquid is good. Therefore, setting the liquid - homogenizing protrusion 6 to 0.05 mm is sufficient.

[0043] Exemplarily, the height of the liquid homogenizing protrusion 6 in this embodiment is 0.1 mm. Correspondingly, at this time, the concentration of the reaction solution added is relatively large, the viscosity of the reaction solution is also relatively large, and the fluidity of the reaction solution is relatively poor. Therefore, the liquid homogenizing protrusion 6 is set to 0.1 mm to increase the liquid homogenizing depth of the reaction solution by the liquid homogenizing protrusion 6, thereby accelerating the mixing of the reaction solution.

[0044] Exemplarily, the height of the liquid homogenizing protrusion 6 can also be 0.07 mm, and the specific height of the liquid homogenizing protrusion 6 is determined according to the actual situation.

[0045] In the present invention, unless otherwise clearly defined and limited, terms such as "installation", "connection", "connection", "fixation", etc. should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium; it can be the communication inside two elements or the interaction relationship between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0046] In the present invention, unless otherwise clearly defined and limited, the first feature being "on" or "under" the second feature can be that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on" the second feature can be that the first feature is directly above or obliquely above the second feature, or merely indicates that the first feature is at a higher horizontal height than the second feature. The first feature being "under", "below" and "beneath" the second feature can be that the first feature is directly below or obliquely below the second feature, or merely indicates that the first feature is at a lower horizontal height than the second feature.

[0047] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those of ordinary skill in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A liquid cover film, comprising a tail plate, a cover plate and a connecting plate, and the tail plate and the connecting plate are respectively arranged at two ends of the cover plate, and a reaction chamber is formed between the cover plate and the glass slide, and is characterized in that, It further includes: A drainage plate, which is arranged in the middle of the tail plate; A first end face, which is arranged on the lower end face of the tail plate and is connected to the end of the drainage plate facing the cover plate; A second end face, which is arranged adjacent to the first end face; Liquid homogenizing protrusions, which are arranged on the second end face, and the number of the liquid homogenizing protrusions is multiple; Drainage grooves, which are formed on the first end face and are arranged on both sides of the drainage plate, and the number of the drainage grooves is multiple.

2. The liquid cover film according to claim 1, wherein: The number of the liquid homogenizing protrusions is an even number, and the even number of the liquid homogenizing protrusions are symmetrically arranged on both sides of the drainage plate.

3. The liquid cover film according to claim 2, wherein, The even number of the liquid homogenizing protrusions are evenly spaced on the second end face.

4. The liquid cover film according to claim 1, wherein, It further includes: Support columns, which are arranged on the tail plate, and there are two support columns, and the two support columns are symmetrically arranged on both sides of the drainage plate; In the horizontal direction, the extending length of the support column is greater than that of the tail plate.

5. The liquid cover film according to claim 1, characterized in that, The number of the drainage grooves is 2, and the 2 drainage grooves are symmetrically arranged on both sides of the drainage plate.

6. The liquid cover film according to claim 5, wherein Along the extending direction of the drainage groove, from top to bottom, the opening degree of the drainage groove gradually increases.

7. The liquid cover film according to claim 6, wherein, The extending direction of the drainage groove is towards the direction gradually away from the drainage plate.

8. The liquid cover film according to any one of claims 1 to 7, characterized in that, The height of the liquid homogenizing protrusion is 0.05 mm - 0.1 mm.

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