Sampling equipment and sampling method for microbial culture medium

Abstract

The present invention belongs to the technical field of microbiological devices, and specifically relates to a sampler for sampling microbial culture media, and more particularly to a sampling device and sampling method for microbial culture media, wherein the sampling device for microbial culture media comprises: an arm body; an auxiliary tooling, which is arranged at the end of the arm body; wherein the auxiliary tooling comprises: a fixed disk, a sleeve, a sampling needle tube, a telescopic member, and a conical mixing member. The telescopic member and the conical mixing member cooperate to assist the sampling needle tube in taking liquid. Before the sampling needle tube takes a sample, the telescopic member first drives the conical mixing member to vortex the liquid in the sampling device for microbial culture media, and at the same time, pushes outward the bubbles generated by the vortex oscillation, thereby ensuring that the sediment in the liquid is uniform while avoiding interference with subsequent sampling caused by bubbles generated by the vortex oscillation.

Description

Technical Field

[0001] The present invention belongs to the technical field of microbiological devices, and in particular relates to a sampler for sampling microbial culture media, and more particularly to a sampling device and a sampling method for microbial culture media. Background Art

[0002] The sampling equipment is used to sample the liquid in the microbial culture medium to facilitate the detection of the liquid in the microbial culture medium and analyze the growth status of the microorganisms in the liquid culture medium.

[0003] In the related art, when sampling liquid in a microbial culture medium, a sampling needle is generally inserted into the culture medium for sampling. To ensure the accuracy of the liquid detection in the microbial culture medium, the liquid in the microbial culture medium needs to be sampled multiple times. When the sampled liquid is tested, the data obtained after each sampling is significantly different, resulting in low accuracy of the final test data.

[0004] Therefore, how to improve the uniformity of sediment dispersion in the liquid sampled each time is a technical problem that needs to be solved urgently.

[0005] It should be noted that the above information disclosed in this background technology section is only used to understand the background technology of the present application concept, and therefore, the above description is not considered to constitute information of the prior art. Summary of the Invention

[0006] The embodiments of the present disclosure at least provide a sampling device for microbial culture medium and a sampling method thereof.

[0007] In a first aspect, the present disclosure provides a sampling device for a microbial culture medium, comprising:

[0008] Arm body;

[0009] An auxiliary tooling, which is arranged at the end of the arm body;

[0010] Wherein, the auxiliary tooling includes:

[0011] a fixed plate, which is arranged at the end of the arm body;

[0012] a sleeve, which is disposed below the fixed disk and communicated with the through hole of the fixed disk;

[0013] a sampling needle, which passes through the through hole of the fixed disk and the sleeve in sequence and is connected to the fixed disk, and is used to absorb liquid in the microbial culture medium;

[0014] a telescopic member, which is sleeved on the sleeve;

[0015] a conical mixing element, which is arranged below the telescopic element and is used to cover the sampling needle tube;

[0016] The telescopic member performs up and down movements to drive the conical mixer to rotate forward and reverse and to rise and fall, so that the lower end of the conical mixer is repeatedly opened and closed to push away the bubbles on the liquid surface of the microbial culture solution. At the same time, the conical mixer rotates to vortex the microbial culture solution.

[0017] In an optional embodiment, the conical mixing element comprises:

[0018] Multiple curved clamping plates;

[0019] The plurality of arc-shaped clamping plates are arranged at intervals along the circumference of the telescopic member and are rotatably connected to the telescopic member via a rotating shaft and a return torsion spring;

[0020] A supporting plate extends outward from the top of the arc-shaped clamping plate;

[0021] A supporting ring is provided below the fixed disk, the supporting ring is spaced apart from the fixed disk, and is connected to the fixed disk via a connecting plate;

[0022] The supporting plate is overlapped on the supporting ring;

[0023] When the telescopic member descends, the end of the arc-shaped clamping plate is driven to open outward by the abutting plate. When the telescopic member ascends, the limit on the abutting plate is released, and the arc-shaped clamping plate is closed inwardly under the drive of the reset torsion spring.

[0024] In an optional embodiment, the sidewall of the arc-shaped clamping plate extends outwardly to form a swirl blade;

[0025] When the telescopic member rotates, the arc-shaped clamping plate is driven to rotate, thereby driving the vortex blade to rotate.

[0026] In an optional embodiment, the side wall of the arc-shaped clamping plate is provided with a fitting surface;

[0027] When the conical mixing element is closed, the abutting surfaces of two adjacent arc-shaped clamping plates abut against each other.

[0028] In an optional embodiment, the end of the arc-shaped clamping plate extends downwardly out of the inserting portion;

[0029] When the conical mixing element is closed, the inserting portions of the plurality of arc-shaped clamping plates are spliced ​​together to form a plug, so as to form a conical cavity inside the conical mixing element;

[0030] The needle tip of the sampling needle tube is located in the conical cavity.

[0031] In an optional embodiment, the abutment ring is concentrically arranged with the sleeve;

[0032] Moreover, the outer diameter of the supporting ring is smaller than the diameter of the bottle mouth of the microbial culture medium.

[0033] In an optional embodiment, the telescopic member includes:

[0034] a telescopic tube, which is sleeved on the sleeve via a return spring;

[0035] The inner wall of the telescopic tube is provided with a spiral slideway;

[0036] The outer wall of the sleeve is provided with a slider adapted to the spiral slideway;

[0037] When the telescopic tube is lifted or lowered by an external force, the spiral slideway cooperates with the slider to rotate, thereby driving the conical mixing element arranged below the telescopic tube to rotate and lift.

[0038] In an optional embodiment, the telescopic tube extends radially outwardly to form a dial ring;

[0039] When the toggle ring is subjected to external force, it drives the telescopic tube to move up and down.

[0040] In a second aspect, the present disclosure also provides a sampling method for the above-mentioned microbial culture medium sampling device, the sampling method comprising:

[0041] Adjust the position of the end of the arm so that the auxiliary tooling is located above the bottle mouth of the microbial culture medium;

[0042] Inserting the auxiliary tool into the microbial culture medium so that the conical mixing piece is inserted into the liquid of the microbial culture medium;

[0043] Repeatedly pressing the telescopic part drives the conical mixing part to rotate and rise and fall, vortexing the liquid in the microbial culture medium and moving the generated bubbles around;

[0044] After pressing the preset number of times, the auxiliary tool is pulled out from the microbial culture medium;

[0045] Inserting the auxiliary tool into the microbial culture medium again so that the conical mixing element is inserted into the liquid of the microbial culture medium;

[0046] Press the telescopic part once to push away the bubbles on the liquid surface, so that the liquid in the middle of the microbial culture medium contacts the needle of the sampling needle tube;

[0047] Sampling is completed by extracting the liquid in the microbial culture medium through a sampling needle.

[0048] In an optional embodiment, the conical mixing element comprises:

[0049] Multiple curved clamping plates;

[0050] The plurality of arc-shaped clamping plates are arranged at intervals along the circumference of the telescopic member and are rotatably connected to the telescopic member via a rotating shaft and a return torsion spring;

[0051] A supporting plate extends outward from the top of the arc-shaped clamping plate;

[0052] A supporting ring is provided below the fixed disk, the supporting ring is spaced apart from the fixed disk, and is connected to the fixed disk via a connecting plate;

[0053] The supporting plate is overlapped on the supporting ring;

[0054] When the telescopic member descends, the end of the arc-shaped clamping plate is driven to open outward by the abutting plate. When the telescopic member ascends, the limit on the abutting plate is released, and the arc-shaped clamping plate is closed inwardly under the drive of the reset torsion spring.

[0055] The beneficial effect of the present invention is that the sampling equipment for microbial culture medium and the sampling method thereof assist the sampling needle in taking liquid through the cooperation of the telescopic part and the conical mixing part. Before the sampling needle takes samples, the conical mixing part is first driven by the telescopic part to vortex-oscillate the liquid in the sampling equipment for microbial culture medium. At the same time, the bubbles generated by the vortex-oscillation are pushed outward. During the vortex-oscillation, there is no need to consider the generation of bubbles, so that the liquid can be fully vortex-oscillated to ensure the uniformity of the dispersion of sediment in the liquid during subsequent sampling.

[0056] Other features and advantages of the present invention are described in the following description, and in part become apparent from the description, or are understood by practicing the present invention. The purpose and other advantages of the present invention are realized and obtained by the structures particularly pointed out in the description and the drawings.

[0057] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, preferred embodiments are specifically cited herein and described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0058] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0059] Figure 1 A schematic diagram of the state of the microbial culture medium sampling device provided in an embodiment of the present disclosure when inserted into the microbial culture medium;

[0060] Figure 2 A schematic diagram of the structure of a sampling device for microbial culture medium provided in an embodiment of the present disclosure;

[0061] Figure 3 A front view of a partial structure of a sampling device for microbial culture medium provided by an embodiment of the present disclosure;

[0062] Figure 4 A cross-sectional view of a sampling device for microbial culture medium provided in an embodiment of the present disclosure;

[0063] Figure 5 Schematic diagram of the closed and opened states of the conical mixing element of the microbial culture medium sampling device provided in an embodiment of the present disclosure;

[0064] Figure 6 A schematic diagram of the structure of a telescopic member of a microbial culture medium sampling device provided in an embodiment of the present disclosure;

[0065] Figure 7 A schematic structural diagram of an arc-shaped clamping plate of a microbial culture medium sampling device provided in an embodiment of the present disclosure;

[0066] Figure 8 A schematic flow chart of a sampling method for a microbial culture medium sampling device provided in an embodiment of the present disclosure;

[0067] Figure 9 This is a schematic diagram of the state of bubbles in the microbial culture medium provided by an embodiment of the present disclosure being pushed outward.

[0068] In the figure: 100, arm body; 200, auxiliary tooling; 210, fixed plate; 211, holding ring; 212, connecting plate; 220, sleeve; 221, slider; 230, sampling needle tube; 231, needle; 240, telescopic member; 241, telescopic tube; 242, return spring; 243, spiral slide; 244, toggle ring; 250, conical mixing element; 251, arc-shaped clamping plate; 251a, rotating shaft; 251b, return torsion spring; 251c, holding plate; 251d, vortex blade; 251e, fitting surface; 251f, insert; 251g, conical cavity; 300, microbial culture medium; 310-liquid surface; 320-bubble. DETAILED DESCRIPTION

[0069] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of them. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0070] In this document, when it is mentioned that a first component is located on a second component, this may mean that the first component may be directly formed on the second component, or that a third component may be interposed between the first component and the second component. In addition, in the drawings, the thickness of components may be exaggerated or reduced in order to effectively describe technical content.

[0071] As used herein, the phrases "in one embodiment," "according to one embodiment," "in some embodiments," and the like generally refer to the fact that the particular feature, structure, or characteristic following the phrase may be included in at least one embodiment of the present disclosure. Thus, a particular feature, structure, or characteristic may be included in more than one embodiment of the present disclosure, such that these phrases do not necessarily refer to the same embodiment. As used herein, the terms "example," "exemplary," and the like are used to "serve as an example, instance, or illustration." Any implementation, aspect, or design described herein as "example" or "exemplary" is not necessarily to be construed as preferred or advantageous over other implementations, aspects, or designs. Instead, the use of the terms "example," "exemplary," and the like is intended to present concepts in a concrete manner.

[0072] Research has found that when testing the liquid of microbial culture medium, it is crucial to ensure that the sediment in the liquid (for example, nutrients, dead microbial cells, inorganic salt crystals, etc.) is evenly dispersed when sampling. Related technologies use manual methods to shake the microbial culture medium. For example, in order to avoid violent shaking to generate bubbles, the microbial culture medium needs to be slowly rotated about 5-10 times. The number of rotations is too small, which is not enough to fully disperse the sediment in the liquid in the microbial culture medium into the liquid. If the number of stirring times is increased, the number of bubbles will increase. When the sampling needle is inserted into the bottle, the bubbles at the liquid surface will affect the accuracy of the sampling volume. Therefore, the shaking can only be performed in a slow rotation manner, and the number of rotations cannot be too many, which will result in inconsistent composition of the liquid sampled each time, affecting the accuracy of subsequent liquid detection.

[0073] Based on the above research, an embodiment of the present disclosure provides a sampling device and a sampling method for a microbial culture medium 300. By pushing away the bubbles 320 at the liquid surface 310 generated by vortex oscillation, the sampling needle 230 is prevented from contacting the bubbles 320 on the liquid surface 310 during sampling, thereby solving the problem in the prior art that bubbles 320 are easily generated when the liquid is fully shaken, thereby affecting sampling.

[0074] The defects in the above solutions are the results obtained by the inventors after practice and careful research. Therefore, the discovery process of the above problems and the solutions proposed by the present disclosure in this article should be the contributions made by the inventors to the present disclosure during the disclosure process.

[0075] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not need to be further defined or explained in subsequent drawings.

[0076] The following embodiments of the present invention are described in detail with reference to the accompanying drawings. In the absence of conflict, the following embodiments and features therein may be combined with each other.

[0077] See also Figure 1 At least one embodiment provides a sampling device for a microbial culture medium 300, comprising the following structure:

[0078] The arm body 100 is provided with a plurality of adjustment arms for adjusting the position of the end of the arm body 100 .

[0079] The auxiliary tooling 200 is provided at the end of the arm body 100 and is used for sampling the liquid in the microbial culture medium 300 .

[0080] See also Figure 2 as well as Figure 3 The auxiliary tooling 200 includes: a fixed disk 210, which is arranged at the end of the arm body 100; a sleeve 220, which is arranged below the fixed disk 210 and is connected to the through hole of the fixed disk 210; a sampling needle tube 230, which passes through the through hole of the fixed disk 210 and the sleeve 220 in sequence and is connected to the fixed disk 210, and is used to absorb the liquid in the microbial culture medium 300; a telescopic member 240, which is sleeved on the sleeve 220; a conical mixing member 250, which is arranged below the telescopic member 240 and is used to cover the sampling needle tube 230; the telescopic member 240 performs an up and down action to drive the conical mixing member 250 to rotate forward and backward and rise and fall, so that the lower end of the conical mixing member 250 is repeatedly opened and closed to remove the bubbles on the liquid surface of the microbial culture solution, and at the same time, the conical mixing member 250 rotates to vortex the microbial culture solution.

[0081] The telescopic member 240 performs an up and down movement, which is achieved by pressing the telescopic member.

[0082] The telescopic member 240 cooperates with the conical mixing member 250 to assist the sampling needle 230 in taking liquid. Before the sampling needle 230 takes samples, the telescopic member 240 drives the conical mixing member 250 to vortex the liquid in the microbial culture medium 300 sampling device. At the same time, the bubbles 320 at the liquid surface 310 generated by the vortex oscillation are pushed outward, thereby ensuring the vortex oscillation effect while avoiding interference with subsequent sampling caused by the bubbles 320 generated by the vortex oscillation.

[0083] The schematic diagram of the state of pushing out the bubbles 320 generated by the vortex oscillation is as follows: Figure 9 shown.

[0084] See also Figure 4 as well as Figure 5 In (a), the conical mixing element 250 includes a plurality of arc-shaped clamping plates 251 .

[0085] The plurality of arc-shaped clamping plates 251 are arranged at intervals along the circumference of the telescopic member 240 and are rotatably connected to the telescopic member 240 via a rotating shaft 251 a and a return torsion spring 251 b .

[0086] The plurality of arc-shaped clamping plates 251 enclose and form a cone, so as to push the liquid surface 310 outwards and further push the air bubbles 320 outwards when the microorganism culture medium 300 is inserted.

[0087] A supporting plate 251c extends outward from the top of the arc-shaped clamping plate 251; a supporting ring 211 is provided below the fixed disk 210, and the supporting ring 211 is spaced apart from the fixed disk 210 and is connected to the fixed disk 210 via a connecting plate 212; the supporting plate 251c is overlapped on the supporting ring 211.

[0088] When the telescopic member 240 descends, the end of the arc-shaped clamping plate 251 is driven to open outward by the supporting plate 251c. When the telescopic member 240 ascends, the limit on the supporting plate 251c is released, and the arc-shaped clamping plate 251 is closed inwardly under the drive of the return torsion spring 251b.

[0089] Since the bottle mouth of the microbial culture medium 300 is in a closed state, the arc-shaped clamping plate 251 of the conical mixing element 250 is set to be open outward at the bottom, which can make the vortex oscillation more uniform, further ensuring that the sediment in the liquid is evenly dispersed during subsequent sampling.

[0090] See also Figure 5 (a) and Figure 7 The sidewalls of the arc-shaped clamping plate 251 extend outwardly to form vortex blades 251d. Rotation of the telescopic member 240 drives the arc-shaped clamping plate 251, which in turn drives the vortex blades 251d. The provision of vortex blades 251d increases the contact area between the arc-shaped clamping plate 251 and the liquid, thereby enhancing the vortex oscillation effect.

[0091] See also Figure 4 The side wall of the arc-shaped clamping plate 251 is provided with a fitting surface 251e; when the conical mixing element 250 is closed, the fitting surfaces 251e of two adjacent arc-shaped clamping plates 251 fit together, reducing the amount of liquid entering the inside of the conical body when the conical mixing element 250 is inserted into the liquid surface.

[0092] Please continue reading Figure 4 In a preferred embodiment, the end of the arc-shaped clamping plate 251 extends downwardly to form an insert 251f; when the conical mixing element 250 is closed, the inserts 251f of multiple arc-shaped clamping plates 251 are spliced ​​to form a plug to form a conical cavity 251g inside the conical mixing element 250; the end of the needle 231 of the sampling needle tube 230 is located in the conical cavity 251g.

[0093] When sampling, first insert the bottom of the conical cavity 251g into the liquid, and make the needle 231 of the sampling needle tube 230 lower than the liquid level in the microbial culture medium 300. When sampling, open the arc clamping plate 251. At this time, the liquid fills the conical cavity 251g, so that the liquid contacts the needle 231, thereby avoiding the needle 231 from contacting the liquid surface when inserted into the liquid surface, thereby reducing the impact of residual bubbles on sampling in the sampling tube.

[0094] See also Figure 4 and Figure 5 In (a), the supporting ring 211 is concentrically arranged with the sleeve 220 ; and the outer diameter of the supporting ring 211 is smaller than the diameter of the bottle mouth of the microbial culture medium 300 , so that the supporting ring 211 can be easily inserted into the microbial culture medium 300 .

[0095] See also Figure 5 (a) and Figure 6 The telescopic member 240 includes: a telescopic tube 241, which is sleeved on the sleeve 220 through a return spring 242; the inner wall of the telescopic tube 241 is provided with a spiral slide 243; the outer wall of the sleeve 220 is provided with a slider 221 adapted to the spiral slide 243; when the telescopic tube 241 is lifted or lowered by an external force, the spiral slide 243 cooperates with the slider 221 to rotate, thereby driving the conical mixing element 250 arranged below the telescopic tube 241 to rotate and lift.

[0096] Specifically, by following Figure 5 In the direction shown by F2 in (a), the telescopic tube 241 is pressed. At this time, the arc-shaped clamping plate 251 moves along Figure 5 In (a), the cone mixer 250 is opened by moving in the direction of F1. At the same time, the cone mixer 250 is rotated synchronously with the telescopic tube 241, and the cone mixer 250 is rotated in the direction of rotation. Figure 4 After the pressure on the telescopic tube 241 is released, the telescopic tube 241 moves along the Figure 5 In (a), the arc-shaped clamping plate 251 rises in the opposite direction of F2. Figure 5In (a), F1 rotates in the opposite direction to close the conical mixer 250. At the same time, the arc-shaped clamping plate 251 and the telescopic tube 241 rotate synchronously, driving the conical mixer 250 to rotate. The rotation direction is the same as Figure 4 The direction shown by F is opposite.

[0097] The status diagram when opening is as follows Figure 5 As shown in (b), the closed state diagram is as follows Figure 5 As shown in (a).

[0098] It should be noted that the telescopic tube 241 is along Figure 5 In (a), the falling and rising edges of F2 Figure 5 When F2 in (a) rises in the opposite direction, the telescopic tube 241 rotates in the opposite direction. When the telescopic tube 241 is repeatedly pressed, the conical mixing element 250 is driven to vortex and oscillate the liquid in the microbial culture medium 300 in a rotation mode that alternates between forward and reverse rotation. At the same time, when the conical mixing element 250 is raised and lowered, the sediment in the liquid can be flipped up and down, so that the sediment is evenly dispersed in the liquid, thereby improving the uniformity of the sediment dispersion in the liquid.

[0099] In order to facilitate pressing the telescopic tube 241 , a toggle ring 244 is extended radially outward from the telescopic tube 241 ; when the toggle ring 244 is subjected to an external force, it drives the telescopic tube 241 to move up and down.

[0100] See also Figure 8 At least one embodiment provides a sampling method for the sampling device for the microbial culture medium 300 as described above, the sampling method comprising:

[0101] Step S110 , adjusting the position of the end of the arm 100 so that the auxiliary tooling 200 is located above the bottle mouth of the microbial culture medium 300 .

[0102] Specifically, by adjusting the position of the arm body 100 , the auxiliary tooling 200 can be inserted into the microorganism culture medium 300 .

[0103] In step S120 , the auxiliary tooling 200 is inserted into the microbial culture medium 300 , so that the conical mixing element 250 is inserted into the liquid of the microbial culture medium 300 .

[0104] In step S130 , the telescopic member 240 is repeatedly pressed to drive the conical mixing member 250 to rotate and rise and fall, thereby vortexing the liquid in the microbial culture medium 300 and moving the generated bubbles 320 to all sides.

[0105] Specifically, by pressing the telescopic member 240 , the conical mixing member 250 is driven to rotate and rise and fall.

[0106] In step S140 , after pressing a preset number of times, the auxiliary tool 200 is pulled out from the microorganism culture medium 300 .

[0107] Specifically, the preset number of times is 2 times per second, and the pressing seconds are 20 seconds.

[0108] In step S150 , the auxiliary tooling 200 is inserted into the microbial culture medium 300 again, so that the conical mixing element 250 is inserted into the liquid of the microbial culture medium 300 .

[0109] In step S180 , the telescopic member 240 is pressed once to push away the bubbles 320 on the liquid surface 310 , so that the liquid in the middle of the microbial culture medium 300 contacts the needle 231 of the sampling needle tube 230 .

[0110] In step S170 , the liquid in the microbial culture medium 300 is extracted through the sampling needle 230 to complete the sampling.

[0111] It should be noted that, in step S120 and step S150 , when the auxiliary tooling 200 is inserted into the microbial culture medium 300 , the conical mixing element 250 is in a closed state.

[0112] In summary, the present invention provides a sampling device for a microbial culture medium 300 and a sampling method thereof. Through the cooperation of the telescopic part 240 and the conical mixing part 250, the sampling needle 230 is assisted to collect liquid. Before the sampling needle 230 collects samples, the conical mixing part 250 is driven by the telescopic part 240 to vortex the liquid in the sampling device for the microbial culture medium 300. At the same time, the bubbles 320 at the liquid surface 310 generated by the vortex oscillation are pushed outward, thereby ensuring the vortex oscillation effect while avoiding interference with subsequent sampling caused by the bubbles 320 generated by the vortex oscillation.

[0113] With the above-described preferred embodiments of the present invention as a guide, and with reference to the above description, relevant personnel are fully capable of making various changes and modifications without departing from the technical scope of this invention. The technical scope of this invention is not limited to the contents of the specification and must be determined according to the scope of the claims.

Claims

1. A sampling device for microbial culture medium, characterized in that: include: Arm body (100); An auxiliary tool (200) is provided at the end of the arm body (100); Wherein, the auxiliary tooling (200) comprises: a fixed plate (210) disposed at the end of the arm body (100); a sleeve (220), which is disposed below the fixed disk (210) and communicates with the through hole of the fixed disk (210); a sampling needle (230), which sequentially passes through the through hole of the fixed disk (210) and the sleeve (220) and is connected to the fixed disk (210), and is used to absorb liquid in the microbial culture medium (300); a telescopic member (240) sleeved on the sleeve (220); a conical mixing element (250), which is disposed below the telescopic element (240) and is used to cover the sampling needle tube (230); The telescopic member (240) performs an up-and-down movement to drive the conical mixing member (250) to rotate forward and reverse and to rise and fall, so that the lower end of the conical mixing member (250) is repeatedly opened and closed to remove bubbles on the liquid surface of the microbial culture solution. At the same time, the conical mixing member (250) rotates to vortex the microbial culture solution. The conical mixing element (250) comprises: a plurality of arc-shaped clamping plates (251); The plurality of arc-shaped clamping plates (251) are arranged at intervals along the circumference of the telescopic member (240), and are rotatably connected to the telescopic member (240) via a rotating shaft (251a) and a return torsion spring (251b); A supporting plate (251c) extends outward from the top of the arc-shaped clamping plate (251); A supporting ring (211) is provided below the fixed disk (210), the supporting ring (211) is spaced apart from the fixed disk (210), and is connected to the fixed disk (210) via a connecting plate (212); The supporting plate (251c) is overlapped on the supporting ring (211); When the telescopic member (240) descends, the end of the arc-shaped clamping plate (251) is driven to open outwards by the supporting plate (251c); when the telescopic member (240) ascends, the position restriction on the supporting plate (251c) is released, and the arc-shaped clamping plate (251) is closed inwards under the drive of the return torsion spring (251b).

2. The microbial culture medium sampling device according to claim 1, wherein: The side wall of the arc-shaped clamping plate (251) extends outward to form a vortex blade (251d); When the telescopic member (240) rotates, it drives the arc-shaped clamping plate (251) to rotate, thereby driving the vortex blade (251d) to rotate.

3. The microbial culture medium sampling device according to claim 1, wherein: The side wall of the arc-shaped clamping plate (251) is provided with a fitting surface (251e); When the conical mixing element (250) is closed, the fitting surfaces (251e) of two adjacent arc-shaped clamping plates (251) fit together.

4. The microbial culture medium sampling device according to claim 1, wherein: The end of the arc-shaped clamping plate (251) extends downwardly to form an inserting portion (251f); When the conical mixing piece (250) is closed, the inserting portions (251f) of the plurality of arc-shaped clamping plates (251) are spliced ​​together to form a plug, thereby forming a conical cavity (251g) inside the conical mixing piece (250); The end of the needle (231) of the sampling needle tube (230) is located in the conical cavity (251g).

5. The microbial culture medium sampling device according to claim 1, wherein: The supporting ring (211) and the sleeve (220) are arranged concentrically; Furthermore, the outer diameter of the supporting ring (211) is smaller than the diameter of the bottle mouth of the microbial culture medium (300).

6. The microbial culture medium sampling device according to claim 1, wherein: The telescopic member (240) comprises: a telescopic tube (241) which is sleeved on the sleeve (220) via a return spring (242); The inner wall of the telescopic tube (241) is provided with a spiral slideway (243); The outer wall of the sleeve (220) is provided with a sliding block (221) adapted to the spiral slideway (243); When the telescopic tube (241) is lifted or lowered by an external force, the spiral slideway (243) cooperates with the slider (221) to achieve rotation, thereby driving the conical mixing element (250) disposed below the telescopic tube (241) to rotate and lift.

7. The microbial culture medium sampling device according to claim 6, wherein: The telescopic tube (241) extends radially outward to form a dial ring (244); When the toggle ring (244) is subjected to an external force, it drives the telescopic tube (241) to move up and down.

8. A sampling method applied to the microbial culture medium sampling device according to claim 1, characterized in that: The sampling method includes: Adjusting the end position of the arm body (100) so that the auxiliary tooling (200) is located above the bottle mouth of the microbial culture medium (300); Inserting the auxiliary tool (200) into the microbial culture medium (300) so that the conical mixing element (250) is inserted into the liquid of the microbial culture medium (300); Repeatedly pressing the telescopic member (240) drives the conical mixing member (250) to rotate and rise and fall, vortexing the liquid in the microbial culture medium (300) and moving the generated bubbles to all sides; After pressing a preset number of times, the auxiliary tool (200) is pulled out from the microbial culture medium (300); Inserting the auxiliary tool (200) into the microbial culture medium (300) again, so that the conical mixing element (250) is inserted into the liquid of the microbial culture medium (300); Pressing the telescopic member (240) once to push away bubbles on the liquid surface, so that the liquid in the middle of the microbial culture medium (300) contacts the needle (231) of the sampling needle tube (230); The liquid in the microbial culture medium (300) is extracted through the sampling needle tube (230) to complete the sampling.

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