Soil carbon sequestration and pollution elimination evaluation device and method based on immobilized microorganisms

Abstract

The invention discloses a soil carbon sequestration and pollution elimination evaluation device and method based on immobilized microorganisms, the device comprises an evaluation table, a rotating frame rotatably arranged on the evaluation table and a plurality of groups of cylinder assemblies arranged on the rotating frame in an annular array, soil sample containers are arranged on the cylinder assemblies, an insertion cylinder is arranged above the rotating frame, and the insertion cylinder is provided with an insertion hole. A first through opening is formed in the outer wall of the insertion cylinder, a detection sensor is embedded in the outer wall of the insertion cylinder, the insertion cylinder is arranged on an annular guide rail in a sliding mode, and the annular guide rail is driven by a linear driving mechanism arranged on the evaluation table; and the gas supply assembly is arranged on the evaluation table. Soil sample containers containing soil samples in the same area are placed through the multiple cylinder assemblies, different immobilized microorganism particles are placed in the insertion cylinders, soil data are collected through the detection sensor, and then evaluation information is generated according to the collected soil data; therefore, the immobilized microorganism particles most suitable for the soil characteristics of the area can be conveniently selected.

Description

Technical Field

[0001] The present invention relates to the technical field of soil pollution remediation, and specifically to a soil carbon sequestration and pollution elimination assessment device and method based on immobilized microorganisms. Background Technique

[0002] The problem of soil pollution by emerging contaminants (ECs; such as antibiotics, endocrine disruptors, microplastics, and persistent organic pollutants) has attracted much attention. These emerging contaminants are enriched in the soil over a long period, not only seriously damaging the soil quality, but also being absorbed by plants and animals and ultimately entering the food chain, posing risks to human health. For soil emerging contaminants, the immobilized microorganism technology is usually adopted at present. The immobilized microorganism technology refers to fixing free microorganisms with specific physiological functions inside or on the surface of carrier materials (biochar, activated carbon, carbon-based nanomaterials, etc.) through physical or chemical means, and then using the immobilized microorganisms to carry out soil carbon sequestration and pollution elimination treatment. However, due to the significant differences in soil environments in different regions, there is currently no scientific and systematic evaluation of immobilized microorganisms before their use, which is not convenient for selecting suitable immobilized microorganisms for the soil area to be remediated, easily leading to the mismatch between microorganisms and the soil environment, reducing the remediation efficiency, and even possibly causing the death of microorganisms due to insufficient adaptability, resulting in waste of resources and secondary pollution. For this reason, we propose a soil carbon sequestration and pollution elimination assessment device and method based on immobilized microorganisms. Summary of the Invention

[0003] The purpose of the present invention is to provide a soil carbon sequestration and pollution elimination assessment device and method based on immobilized microorganisms to solve the problems raised in the above background technique.

[0004] To achieve the above purpose, the present invention provides the following technical solutions:

[0005] A soil carbon sequestration and pollution elimination assessment device based on immobilized microorganisms includes an assessment table, a rotating frame rotatably arranged on the assessment table, and a plurality of groups of cylinder assemblies arranged in an annular array on the rotating frame. A soil sample container for placing soil samples is provided on the cylinder assembly. Above the rotating frame, there are a plurality of groups of insertion cylinders corresponding to the cylinder assemblies one by one and used for accommodating immobilized microorganism particles. A first through hole is opened on the outer wall of the insertion cylinder, and a detection sensor for collecting soil data is embedded on the outer wall of the insertion cylinder. The detection sensor is electrically connected to a controller, and the controller is electrically connected to an assessment system. The insertion cylinder is slidably arranged on an annular guide rail, and the annular guide rail is driven by a linear driving mechanism arranged on the assessment table to move vertically relative to the rotating frame;

[0006] It also includes a gas supply assembly arranged on the assessment table for injecting gas into one of the cylinder assemblies to heat or cool the soil sample in the soil sample container when the rotating frame rotates to a preset position.

[0007] A further improvement lies in that the evaluation system includes a comparison module electrically connected to the controller and an evaluation module connected to the comparison module. The comparison module is used to compare the soil data collected by the detection sensor with the historical soil data to obtain a comparison result, and the evaluation module receives the comparison result to generate evaluation information.

[0008] A further improvement lies in that the cylinder assembly includes:

[0009] An inner cylinder, sleeved outside the soil sample container, with an outer cylinder rotatably sleeved on its outer wall. The top and bottom of the inner cylinder are respectively connected with an air outlet pipe and an air inlet pipe;

[0010] A rotating support platform, rotatably arranged on the bottom wall of the inner cylinder and clamped with the soil sample container. The rotating support platform is rotatably connected to the shaft part of the impeller part. The impeller part of the impeller part is located in the guide seat. One end of the guide seat is communicated with the inner cavity of the inner cylinder, and the other end is communicated with the air inlet pipe.

[0011] A further improvement lies in that the gas supply assembly includes:

[0012] A shunt seat, with two cavities respectively opened at the upper and lower ends inside it. Two butt joint pipes respectively inserted into the upper and lower ends on one side of it and communicated with the two cavities are used to be respectively communicated with the air outlet pipe and the air inlet pipe in a cylinder assembly. The upper cavity is communicated with a filtering device through a pipeline, and the lower cavity is communicated with a control valve through a pipeline. And the two input ends of the control valve are respectively communicated with a heating device and a refrigerating device through pipelines.

[0013] A further improvement lies in that the ends of the air outlet pipe and the air inlet pipe far from the inner cylinder are both communicated with a butt joint sleeve for the butt joint pipe to enter. A magnetic block is embedded on the inner wall of the butt joint sleeve, and an electromagnet block for electrically adsorbing the magnetic block is embedded on the outer wall of the end of the butt joint pipe. An elastic member is arranged at the connection between the butt joint pipe and the shunt seat. A through hole for the butt joint pipe to pass through is opened on the outer wall of the butt joint sleeve along the rotation direction of the rotating frame and far from the butt joint pipe. A rotating block for closing the through hole is hinged in the through hole through an elastic connecting member.

[0014] A further improvement lies in that a plurality of groups of through holes one are respectively opened on the circumferential outer walls of the outer cylinder and the inner cylinder. An elastic reset member is arranged at the connection between the outer cylinder and the inner cylinder. A gear is sleeved on the outer wall of the outer cylinder. An arc-shaped rack is arranged on the evaluation platform and is upstream of the butt joint pipe. Before a cylinder assembly rotates with the rotating frame to make the air outlet pipe and the air inlet pipe correspond to the butt joint pipe, the gear in this cylinder assembly is driven by the arc-shaped rack to drive the outer cylinder to rotate, so that the through holes one on the outer cylinder and the inner cylinder are staggered from each other. When the gear disengages from the arc-shaped rack, the elastic reset member drives the outer cylinder to rotate back to its original position, so that the through holes one on the outer cylinder and the inner cylinder correspond to each other.

[0015] A further improvement lies in that contact blocks are provided on the outer wall of the inner cylinder, baffles are provided on the outer wall of the shunt base, the baffles are in contact with the contact blocks when the air outlet pipe, the air inlet pipe and the docking pipe are corresponding, pressure sensors are embedded on the baffles, the pressure sensors are connected to a controller, and the controller is connected to an electromagnetic block. The baffles are hinged on the outer wall of the shunt base, and the baffles and the shunt base are connected by arc-shaped springs.

[0016] A further improvement lies in that a cylinder cover is hinged to the top of the insertion cylinder.

[0017] A further improvement lies in that the controller is arranged on one side of the evaluation table, and the controller is also electrically connected to the rotating frame, the linear driving mechanism and the gas supply assembly.

[0018] The soil carbon sequestration and pollution reduction evaluation method based on immobilized microorganisms uses the above-mentioned evaluation device and includes the following steps:

[0019] S1: Place several soil samples collected from the same area in several groups of soil sample containers respectively, and then place the soil sample containers in the cylinder assembly;

[0020] S2: Place different immobilized microorganism particles in several insertion cylinders respectively. Drive the insertion cylinders to insert into the corresponding soil sample containers through the linear driving mechanism. Automatically collect soil data by the detection sensor within a set period, and send the soil data to the controller, and then send it to the evaluation system through the controller to generate evaluation information. Among them, the rotating frame can be regularly controlled to rotate according to a preset program, so that different cylinder assemblies intermittently correspond to the gas supply assembly, and gas is injected into the corresponding cylinder assemblies through the gas supply assembly to heat or cool the soil samples in the soil sample containers, so as to obtain the soil data of the treatment of the soil by different immobilized microorganism particles in soils at different temperatures.

[0021] Compared with the prior art, the beneficial effects of the present invention are:

[0022] The present invention places soil sample containers containing soil samples from the same area through multiple cylinder assemblies, places different immobilized microorganism particles in the insertion cylinders, inserts the insertion cylinders into the soil sample containers to collect soil data through the detection sensor, and then generates evaluation information according to the collected soil data, so as to conveniently select the immobilized microorganism particles most suitable for the soil characteristics of this area, realize precise and intelligent screening of soil remediation microorganisms, greatly improve the scientificity of the formulation of the remediation plan and the remediation efficiency, and the gas supply assembly can supply gas into the cylinder assembly to heat or cool the soil samples in the soil sample containers, so as to comprehensively evaluate the treatment effect of the immobilized microorganisms on the soil samples in soils at different temperatures. Description of the Drawings

[0023] Figure 1Schematic structural diagram of the evaluation device of the present invention;

[0024] Figure 2 of the present invention Figure 1 Rear view of the structure;

[0025] Figure 3 Schematic structural diagram of the cylinder assembly of the present invention;

[0026] Figure 4 of the present invention Figure 3 Structural cross-sectional view;

[0027] Figure 5 of the present invention Figure 1 Enlarged view of structure A in the present invention.

[0028] In the figure: 1, evaluation table; 2, rotating frame; 3, inner cylinder; 4, soil sample container; 5, outer cylinder; 6, first through port; 7, gear; 8, intake pipe; 9, exhaust pipe; 10, docking sleeve; 11, rotating block; 12, guiding seat; 13, impeller member; 14, rotating support table; 15, annular guide rail; 16, insertion cylinder; 17, second through port; 18, detection sensor; 19, linear driving mechanism; 20, arc-shaped rack; 21, shunt seat; 22, docking pipe; 23, baffle; 24, arc-shaped spring; 25, filtering device; 26, heating device; 27, refrigeration device; 28, controller. Specific embodiments

[0029] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0030] Embodiment 1

[0031] Please refer to the attached Figure 1 - attached Figure 2 , a soil carbon sequestration and pollution reduction evaluation device based on immobilized microorganisms, including an evaluation table 1 with an L-shaped vertical section, a rotating frame 2 rotatably arranged on the evaluation table 1, and a plurality of groups of cylinder assemblies arranged on the rotating frame 2 in an annular array, and a soil sample container 4 for placing soil samples is provided on the cylinder assembly. The rotating frame 2 is an annular frame driven by a motor to rotate. As shown in the attached Figure 1 figure, the rotating frame 2 includes a motor (not shown in the figure) embedded in the evaluation table 1, a chassis arranged at the output end of the motor, a top plate arranged above the chassis, and a support rod connecting the chassis and the top plate;

[0032] Above the rotating frame 2, there are several groups of insertion cylinders 16 corresponding to the cylinder components one by one and used to accommodate immobilized microbial particles. The immobilized microbial particles are obtained by immobilizing microorganisms on carrier materials by methods such as adsorption method, embedding method, covalent binding method or crosslinking method. The carrier materials are, for example, biochar, activated carbon, carbon nanotubes, carbon-based nanomaterials, etc. For example, biochar is attached with Sphingomonas, laccase, Bacillus, Microbacterium or Rhodococcus biphenylivorans, etc., and activated carbon is attached with Cupriavidus or laccase, etc.;

[0033] On the outer wall of the insertion cylinder 16, there is a first through opening 6. The insertion cylinder 16 enters the soil sample container 4. The soil in the soil sample container 4 contacts the immobilized microbial particles through the first through opening 6 and is treated by the immobilized microbial particles. Embedded in the outer wall of the insertion cylinder 16 is a detection sensor 18 for collecting soil data. The detection sensor 18 includes an electrochemical sensor, a soil CO2 flux sensor, a soil temperature sensor, etc. The above sensors are all conventional devices in the art, and their models are selected according to the actual situation and will not be elaborated here;

[0034] The detection sensor 18 is electrically connected to the controller 28, and the controller 28 is electrically connected to the evaluation system. The insertion cylinder 16 is slidably arranged on the annular guide rail 15. The insertion cylinder 16 is slidably connected to the annular guide rail 15 through the cooperation of the slider on its outer wall and the chute of the annular guide rail 15. After the insertion cylinder 16 is inserted into the corresponding soil sample container 4, it can perform a circular motion with the soil sample container 4. The annular guide rail 15 is driven by a linear driving mechanism 19 arranged on the evaluation table 1 to move vertically relative to the rotating frame 2. The linear driving mechanism 19 includes, for example, a vertical electric guide rail and a slider arranged in the vertical electric guide rail and connected to the annular guide rail 15. Of course, the linear driving mechanism 19 is not limited to this kind of device;

[0035] It also includes a gas supply component arranged on the evaluation table 1 for injecting gas into one of the cylinder components to heat or cool the soil sample in the soil sample container 4 when the rotating frame 2 rotates to a preset position.

[0036] Preferably, the evaluation system of this embodiment includes a comparison module electrically connected to the controller 28 and an evaluation module connected to the comparison module. The comparison module is used to compare the soil data collected by the detection sensor 18 with the historical soil data to obtain a comparison result. The evaluation module receives the comparison result and generates an evaluation message;

[0037] For example, compare the detection values of various pollutants in the soil. If the collected detection value is less than the detection value of the historical soil data, the comparison result is excellent. If the detection value is greater than or equal to the detection value of the historical soil data, the comparison result is poor;

[0038] For the soil carbon sequestration data detection value, if the collected detection value is greater than the detection value of the historical soil data, an excellent comparison result is generated; if the collected detection value is less than or equal to the detection value of the historical soil data, a poor comparison result is generated.

[0039] According to the comparison result, the evaluation information of the corresponding immobilized microbial particles for the soil sample can be generated, including the pollutant removal performance, carbon sequestration ability, and environmental adaptability of the immobilized microbial particles.

[0040] Preferably, a cylinder cover is hinged to the top of the insertion cylinder 16 in this embodiment. After the insertion cylinder 16 is inserted into the soil sample container 4, the cylinder cover closes the top of the insertion cylinder 16 so that the immobilized microbial particles can process the soil sample in the soil sample container 4.

[0041] The soil carbon sequestration and pollution reduction evaluation method based on immobilized microorganisms, using the above evaluation device, includes the following steps:

[0042] S1: Place several soil samples collected from the same area in several groups of soil sample containers 4 respectively, and then place the soil sample containers 4 in the cylinder body assembly.

[0043] S2: Place different immobilized microbial particles in several insertion cylinders 16 respectively. Drive the insertion cylinders 16 to insert into the corresponding soil sample containers 4 through the linear drive mechanism 19. Automatically collect soil data through the detection sensor 18 within a set period, send the soil data to the controller 28, and then send it to the evaluation system through the controller 28 to generate evaluation information. Among them, the rotating frame 2 can be regularly controlled to rotate according to a preset program, so that different cylinder body assemblies intermittently correspond to the gas supply assembly, and the gas supply assembly injects gas into the corresponding cylinder body assembly to heat or cool the soil sample in the soil sample container 4, so as to obtain the soil data of different immobilized microbial particles treating the soil at different temperatures.

[0044] Embodiment 2

[0045] Please refer to the attached Figure 3 - attached Figure 4 On the basis of Embodiment 1, the cylinder body assembly of this embodiment includes:

[0046] The inner cylinder 3 is sleeved outside the soil sample container 4, and its outer wall is rotatably sleeved with an outer cylinder 5 through a bearing. A sealing ring can be arranged at the connection between the inner cylinder 3 and the soil sample container 4 to ensure the sealing performance of the connection between the two. The top and bottom of the inner cylinder 3 are respectively communicated with an air outlet pipe 9 and an air inlet pipe 8. The gas enters the inner cavity of the inner cylinder 3 through the air inlet pipe 8 and then discharges from the air outlet pipe 9.

[0047] Rotate the support platform 14, which is rotatably arranged on the bottom wall of the inner cylinder 3 through a bearing and is clamped with the soil sample container 4. The two can be connected by means of a snap connection. The rotating support platform 14 is rotatably connected to the shaft part of the impeller member 13. The impeller part of the impeller member 13 is located inside the guide seat 12. One end of the guide seat 12 communicates with the inner cavity of the inner cylinder 3, and the other end communicates with the intake pipe 8. The gas entering from the intake pipe 8 will enter the guide seat 12 and then enter the inner cavity of the inner cylinder 3. When the gas flows in the guide seat 12, it will drive the impeller member 13 to rotate. Furthermore, the impeller member 13 drives the rotating support platform 14, and the rotating support platform 14 drives the soil sample container 4 to rotate. On the one hand, it makes the soil in the soil sample container 4 better contact with the immobilized microbial particles inserted into the insertion cylinder 16 to treat the soil sample through the immobilized microbial particles. On the other hand, it makes the soil in the soil sample container 4 better heated or cooled by the gas to quickly change the soil temperature in the soil sample container 4.

[0048] Embodiment 3

[0049] Please refer to the attached Figure 2 - attached Figure 5 , on the basis of Embodiment 2, the gas supply assembly of this embodiment includes:

[0050] The flow dividing seat 21 has two cavities (not shown in the figure) opened at the upper and lower ends inside. Two connecting pipes 22 communicating with the two cavities are respectively inserted at the upper and lower ends on one side. The two connecting pipes 22 are used to communicate with the outlet pipe 9 and the intake pipe 8 in a cylinder assembly respectively. By controlling the rotation of the rotating frame 2 by a preset angle, for example, as shown in the attached Figure 1 figure, if there are four groups of cylinder assemblies, then by controlling the rotating frame 2 to rotate 90 degrees each time, one cylinder assembly can be made to correspond to the two connecting pipes 22;

[0051] The upper cavity is connected with a filtering device 25 through a pipeline, and the lower cavity is connected with a control valve through a pipeline. The two input ends of the control valve are respectively connected with a heating device 26 and a refrigerating device 27 through pipelines. The filtering device 25, the heating device 26 and the refrigerating device 27 are all conventional devices in the art. The filtering device 25 is used to filter the gas, the heating device 26 is used to supply hot gas, such as a gas heater, etc., and the refrigerating device 27 is used to supply cold gas, such as a gas cooler, etc.

[0052] By making different cylinder assemblies correspond to the outlet pipe 9 and the intake pipe 8, and docking the outlet pipe 9 and the intake pipe 8 with the two connecting pipes 22, the soil in the soil sample container 4 can be heated by supplying hot gas through the heating device 26, and the soil in the soil sample container 4 can be cooled by supplying cold gas through the refrigerating device 27. Simulate the removal effect of the immobilized microbial particles in the soil sample container 4 on soil pollutants and the effect of enhancing the carbon sequestration ability at different temperatures, improve the accuracy of the evaluation results, and provide a more accurate basis for actual soil remediation projects.

[0053] Preferably, at one end of the air outlet pipe 9 and the air inlet pipe 8 of this embodiment away from the inner cylinder 3, a docking sleeve 10 for the docking pipe 22 to enter is connected. A magnetic block (which can be made of a metal material) is embedded in the inner wall of the docking sleeve 10, and an electromagnet block for electrically adsorbing the magnetic block is embedded in the outer wall of the end of the docking pipe 22. An elastic member (such as a spring) is provided at the connection between the docking pipe 22 and the flow dividing seat 21. A through port for the docking pipe 22 to pass through is opened on the outer wall of the docking sleeve 10 along the rotation direction of the rotating frame 2 and away from the docking pipe 22. A rotating block 11 for closing the through port is hinged in the through port through an elastic connecting member (such as including a rotating shaft and a torsion spring provided on the rotating shaft);

[0054] For attachment Figure 1 -attachment Figure 2 As shown, the rotating frame 2 rotates clockwise and stops after rotating a preset angle (90 degrees). At this time, a cylinder assembly corresponds to the two docking pipes 22. By turning on the electromagnet block, the two docking pipes 22 respectively enter the corresponding docking sleeves 10 and contact the magnetic blocks. At this time, gas can be supplied by turning on the heating device 26 or the refrigeration device 27. The gas enters the inner cylinder 3 and then is discharged through the air outlet pipe 9, the other docking pipe 22, and the filtering device 25. After processing, the rotating frame 2 continues to rotate. At this time, the docking pipe 22 contacts the rotating block 11, and the cylinder assembly moves with the rotating frame 2. Then the docking pipe 22 causes the rotating block 11 to flip, and the end of the docking pipe 22 disengages from the docking sleeve 10 at the through port. After disengagement, the rotating block 11 resets under the action of the elastic connecting member to close the through port.

[0055] Preferably, a number of groups of through ports 6 are opened on the circumferential outer walls of the outer cylinder 5 and the inner cylinder 3 of this embodiment. An elastic reset member (such as a torsion spring) is provided at the connection between the outer cylinder 5 and the inner cylinder 3. A gear 7 is sleeved on the outer wall of the outer cylinder 5, and an arc-shaped rack 20 is provided on the evaluation table 1. The arc-shaped rack 20 is upstream of the docking pipe 22. Before a cylinder assembly rotates with the rotating frame 2 to make the air outlet pipe 9 and the air inlet pipe 8 correspond to the docking pipe 22, the gear 7 in this cylinder assembly is driven by the arc-shaped rack 20 to drive the outer cylinder 5 to rotate, so that the through ports 6 on the outer cylinder 5 and the inner cylinder 3 are staggered from each other. When the gear 7 disengages from the arc-shaped rack 20, the elastic reset member drives the outer cylinder 5 to reset and rotate, so that the through ports 6 on the outer cylinder 5 and the inner cylinder 3 correspond to each other;

[0056] The through port one 6 in the outer cylinder 5 and the inner cylinder 3 that does not correspond to the docking pipe 22 is in a corresponding state to ensure that the soil in the soil sample container 4 is at a normal temperature. Before it moves to the docking pipe 22 along with the rotating frame 2, the outer cylinder 5 is driven to rotate relative to the inner cylinder 3 through the cooperation of the arc-shaped rack 20 and the gear 7, so that the through port one 6 on the outer cylinder 5 and the inner cylinder 3 are staggered from each other. Furthermore, the gas supplied into the inner cylinder 3 by the subsequent heating device 26 or the refrigeration device 27 will not be discharged from the through port one 6, so that the soil in the soil sample container 4 can be better heated or cooled. When the gear 7 disengages from the arc-shaped rack 20, the outer cylinder 5 resets, so that the soil in the soil sample container 4 can quickly return to the normal temperature.

[0057] Preferably, a contact block is provided on the outer wall of the inner cylinder 3 of this embodiment, and a baffle 23 is provided on the outer wall of the shunt seat 21. The baffle 23 contacts the contact block when the air outlet pipe 9 and the air inlet pipe 8 correspond to the docking pipe 22. A pressure sensor (not shown in the figure) is embedded in the baffle 23. The pressure sensor is connected to the controller 28, and the controller 28 is connected to the electromagnetic block. The baffle 23 is hinged on the outer wall of the shunt seat 21, and the baffle 23 and the shunt seat 21 are connected by an arc-shaped spring 24;

[0058] The rotating frame 2 rotates clockwise and stops after rotating a preset angle (90 degrees). At this time, a cylinder assembly corresponds to the two docking pipes 22, and the contact block contacts the baffle 23. At this time, the pressure sensor sends a signal to the controller 28 under the pressure of the contact block. The controller 28 energizes the electromagnetic block, and then the two docking pipes 22 move into the corresponding docking sleeves 10. After processing, the rotating frame 2 continues to rotate. At this time, the contact block causes the baffle 23 to rotate and squeeze the arc-shaped spring 24, and then the contact block separates from the baffle 23. After the pressure sensor loses pressure, it sends a signal to the controller 28. The controller 28 de-energizes the electromagnetic block, and then the docking pipe 22 resets, thus completing the work of automatic docking.

[0059] Preferably, the controller 28 of this embodiment is provided on one side of the evaluation table 1. The controller 28 is also electrically connected to the rotating frame 2, the linear driving mechanism 19, and the gas supply assembly to control the electrical components in this device. The controller 28 is a conventional device in the art and will not be described in detail here.

[0060] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirits of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. Soil carbon sequestration and pollution reduction assessment device based on immobilized microorganisms, characterized in that: It includes an evaluation table (1), a rotating frame (2) rotatably arranged on the evaluation table (1), and several groups of cylinder assemblies arranged in an annular array on the rotating frame (2). A soil sample container (4) for placing soil samples is provided on the cylinder assembly. Above the rotating frame (2), there are several groups of insertion cylinders (16) corresponding to the cylinder assemblies one by one and used for accommodating immobilized microbial particles. A first through opening (6) is formed in the outer wall of the insertion cylinder (16). A detection sensor (18) for collecting soil data is embedded in the outer wall of the insertion cylinder (16). The detection sensor (18) is electrically connected to a controller (28), and the controller (28) is electrically connected to an evaluation system. The insertion cylinder (16) is slidably arranged on an annular guide rail (15), and the annular guide rail (15) is driven by a linear driving mechanism (19) arranged on the evaluation table (1) to vertically move relative to the rotating frame (2). It further includes a gas supply assembly arranged on the evaluation table (1) for injecting gas into one of the cylinder assemblies to heat or cool the soil sample in the soil sample container (4) when the rotating frame (2) rotates to a preset position.

2. The evaluation device according to claim 1, wherein: The evaluation system includes a comparison module electrically connected to the controller (28) and an evaluation module connected to the comparison module. The comparison module is used to compare the soil data collected by the detection sensor (18) with historical soil data to obtain a comparison result, and the evaluation module receives the comparison result and generates an evaluation message.

3. The evaluation device according to claim 1, characterized in that: The cylinder assembly includes: An inner cylinder (3) sleeved outside the soil sample container (4). An outer cylinder (5) is rotatably sleeved on its outer wall. An air outlet pipe (9) and an air inlet pipe (8) are respectively communicated with the top and bottom of the inner cylinder (3). A rotating support platform (14) rotatably arranged on the bottom wall of the inner cylinder (3) and clamped with the soil sample container (4). The rotating support platform (14) is rotatably connected to the shaft part of an impeller member (13). The impeller part of the impeller member (13) is located in a guide seat (12). One end of the guide seat (12) is communicated with the inner cavity of the inner cylinder (3), and the other end is communicated with the air inlet pipe (8).

4. The evaluation device according to claim 3, characterized in that: The gas supply assembly includes: A flow dividing seat (21) with two cavities respectively opened at its upper and lower ends inside. Two butt joint pipes (22) respectively inserted at the upper and lower ends on one side of it and communicated with the two cavities are used to be respectively communicated with the air outlet pipe (9) and the air inlet pipe (8) in one of the cylinder assemblies. The upper cavity is communicated with a filtering device (25) through a pipeline, and the lower cavity is communicated with a control valve through a pipeline. Two input ends of the control valve are respectively communicated with a heating device (26) and a refrigerating device (27) through pipelines.

5. The evaluation device according to claim 4, characterized in that: One end of the air outlet pipe (9) and the air inlet pipe (8) far away from the inner cylinder (3) is communicated with a docking sleeve (10) for the docking pipe (22) to enter. A magnetic block is embedded in the inner wall of the docking sleeve (10), and an electromagnetic block for electrically adsorbing the magnetic block is embedded in the outer wall of the end of the docking pipe (22). An elastic member is provided at the connection between the docking pipe (22) and the flow dividing seat (21). A through port for the docking pipe (22) to pass through is formed in the outer wall of the docking sleeve (10) along the rotation direction of the rotating frame (2) and away from the docking pipe (22). A rotating block (11) for closing the through port is hinged in the through port through an elastic connecting member.

6. The evaluation device according to claim 3, characterized in that: A plurality of groups of first through ports (6) are formed in the circumferential outer walls of the outer cylinder (5) and the inner cylinder (3). An elastic reset member is provided at the connection between the outer cylinder (5) and the inner cylinder (3). A gear (7) is sleeved on the outer wall of the outer cylinder (5). An arc-shaped rack (20) is provided on the evaluation table (1). The arc-shaped rack (20) is upstream of the docking pipe (22). Before a cylinder assembly rotates with the rotating frame (2) to make the air outlet pipe (9) and the air inlet pipe (8) correspond to the docking pipe (22), the gear (7) in the cylinder assembly is driven by the arc-shaped rack (20) to drive the outer cylinder (5) to rotate, so that the first through ports (6) on the outer cylinder (5) and the inner cylinder (3) are staggered from each other. When the gear (7) disengages from the arc-shaped rack (20), the elastic reset member drives the outer cylinder (5) to rotate back to its original position, so that the first through ports (6) on the outer cylinder (5) and the inner cylinder (3) correspond to each other.

7. The evaluation device according to claim 4, characterized in that: A contact block is provided on the outer wall of the inner cylinder (3). A baffle (23) is provided on the outer wall of the flow dividing seat (21). The baffle (23) contacts the contact block when the air outlet pipe (9) and the air inlet pipe (8) correspond to the docking pipe (22). A pressure sensor is embedded in the baffle (23). The pressure sensor is connected to a controller (28), and the controller (28) is connected to the electromagnetic block. The baffle (23) is hinged on the outer wall of the flow dividing seat (21), and the baffle (23) and the flow dividing seat (21) are connected by an arc-shaped spring (24).

8. The evaluation device according to claim 1, characterized in that: A cylinder cover is hinged to the top of the insertion cylinder (16).

9. The evaluation device according to claim 1, wherein: The controller (28) is arranged on one side of the evaluation table (1). The controller (28) is also electrically connected to the rotating frame (2), the linear driving mechanism (19), and the gas supply assembly.

10. A method for evaluating soil carbon sequestration and pollution reduction based on immobilized microorganisms, using the evaluation device according to any one of claims 1-9, characterized in that: Including the following steps: S1: Place a plurality of soil samples collected from the same area in a plurality of groups of soil sample containers (4) respectively, and then place the soil sample containers (4) in the cylinder assembly; S2: Place different immobilized microbial particles in several insertion cylinders (16) respectively. Drive the insertion cylinders (16) to insert into the corresponding soil sample containers (4) through a linear drive mechanism (19). Automatically collect soil data through a detection sensor (18) within a set period, and send the soil data to a controller (28), and then send it to an evaluation system through the controller (28) to generate evaluation information. Among them, the rotating frame (2) can be periodically controlled to rotate according to a preset program, so that different cylinder components intermittently correspond to the gas supply components, and the gas supply components inject gas into the corresponding cylinder components to heat or cool the soil samples in the soil sample containers (4), so as to obtain the soil data of the soil treatment by different immobilized microbial particles in soils at different temperatures.

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