Incubation device for immunohistochemistry

Abstract

The invention discloses an incubation device for immunohistochemistry, and relates to the technical field of incubation devices.The incubation device comprises a box body, a box cover located on the box body, a plurality of sets of glass slides placed on a placing frame and tissue arranged at the tops of the glass slides, a blocking mechanism is arranged on the box body, and the blocking mechanism comprises a covering assembly; the covering assembly comprises a cover plate arranged above the glass slide, and a rubber plate is fixedly arranged at the bottom of the cover plate; through the designed blocking mechanism, the blocking mechanism effectively covers a primary antibody solution and tissue on the glass slide through the covering assembly, in the process that the incubator is transferred into a refrigerator, if accidental collision or falling occurs, the hydrophobic membrane can utilize the hydrophobic property of the hydrophobic membrane, and the primary antibody solution and tissue on the glass slide can be separated. The splashing solution forms water drops on the surface and slides back into the placing groove of the glass slide, so that the waste of the solution and the pollution to the adjacent glass slide and the box body are avoided.

Description

Technical Field

[0001] The present invention belongs to the technical field of incubation devices, and specifically relates to an incubation device for immunohistochemistry. Background Art

[0002] Immunohistochemistry, also known as immunocytochemistry, is a technique based on the principle of specific binding between antigens and antibodies, which uses labeled antibodies to detect the expression of target proteins in tissue or cell in situ. The incubation of the primary antibody, as the core step of IHC, directly determines the specificity and sensitivity of the detection. During the incubation process, the primary antibody incubation is carried out first. The primary antibody incubation is carried out by placing the glass slide on the placement rack in the incubation box, then placing the sliced tissue on the glass slide, then dropping the primary antibody solution onto the glass slide to cover the tissue, and finally covering the box cover on the box body and placing it in a refrigerator at 4 degrees Celsius for overnight incubation.

[0003] In the prior art, in the immunohistochemistry incubation experiment, when the incubation box is transferred to the refrigerator, if an accidental collision or fall occurs, it is easy for the primary antibody solution on the glass slide to splash. The splashed primary antibody solution not only causes waste, resulting in insufficient solution covering the tissue, but also pollutes the adjacent glass slides and the box body and box cover, affecting the incubation effect. Therefore, we propose an incubation device for immunohistochemistry. Summary of the Invention

[0004] The purpose of the present invention is to provide an incubation device for immunohistochemistry to solve the technical problems raised in the above background art.

[0005] To achieve the above purpose, the present invention provides the following technical solution: An incubation device for immunohistochemistry, including a box body, a box cover located on the box body, multiple groups of glass slides placed on the placement rack, and tissues arranged on the tops of the glass slides. A blocking mechanism is arranged on the box body, and the blocking mechanism includes:

[0006] A covering component, including a cover plate arranged above the glass slide. A rubber plate is fixedly arranged at the bottom of the cover plate, and a hydrophobic film is fixedly arranged at the bottom of the rubber plate, which is used to cover the primary antibody solution and tissue placed on the glass slide;

[0007] Connecting plates are fixedly arranged between multiple groups of the cover plates. A turning member is arranged between the top of the cover plate and the box body. The turning member includes multiple groups of vertical bars and a rear plate fixed to the inner wall of the box body.

[0008] Preferably, the blocking mechanism further includes a lifting component for driving the glass slide to move up and down;

[0009] The lifting component includes a lifting screw rod and a limiting rod. A support bearing is arranged on the vertical bar, and a threaded sleeve is rotatably arranged inside the support bearing.

[0010] Preferably, a hollow block is fixedly arranged on the vertical bar, a rotating rod is rotatably arranged inside the hollow block, a worm gear is arranged outside the threaded sleeve, and two groups of worm shafts are fixedly arranged outside the rotating rod.

[0011] Preferably, the other end of the vertical bar is fixedly provided with a hollow T-shaped rod, and a rotating rod is rotatably arranged inside the hollow T-shaped rod.

[0012] Preferably, a front frame is fixedly arranged on a plurality of groups of hollow T-shaped rods, a plurality of groups of embedding grooves are formed in the top of the front surface of the box body, and two groups of sponge blocks are fixedly arranged on the inner wall of the embedding grooves;

[0013] A rear locking screw is connected to the vertical bar by a thread, and a front locking screw is connected to the front frame by a thread.

[0014] Preferably, a placement groove is formed inside the glass slide, a limiting member for limiting the tissue is arranged inside the glass slide, the limiting member includes two groups of long blocking frames and two groups of short blocking frames, magnets are fixedly arranged on both sides of the inner wall of the placement groove, and iron plates are fixedly arranged at both ends of the long blocking frame.

[0015] Preferably, two groups of medical-grade silica gel plates are fixedly arranged on the two end surfaces of the short blocking frame, and two groups of blocking bars are rotatably arranged on the short blocking frame through damping rotating shafts.

[0016] Preferably, the blocking mechanism includes a plate frame arranged above the glass slide and a movable frame fixed on the top of the plate frame, and a cover glass is fixedly arranged at the bottom of the plate frame.

[0017] Preferably, a vertical block is fixedly arranged on the top of the movable frame, a vertical plate is fixedly arranged on the top of the box cover, and a spiral column is connected to the inside of the vertical plate by a thread.

[0018] Preferably, the placement rack is fixed on the inner wall of the box body, and side plates are fixedly arranged on both sides of the box cover.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0020] (1) Through the designed blocking mechanism of the present invention, the blocking mechanism effectively covers the primary antibody solution and the tissue on the glass slide through the covering assembly. During the process of transferring the incubator to the refrigerator, if an accidental collision or drop occurs, the hydrophobic film can utilize its hydrophobic property to make the splashed solution form water droplets on its surface and slide back into the placement groove of the glass slide, thereby avoiding the waste of the solution and the pollution of adjacent glass slides and the box body. The cover plate can be flipped to a vertical state, which does not affect the operator's normal operation of taking and placing tissues, improves the incubation efficiency, and can also press and limit the glass slides placed on the placement rack to prevent them from moving.

[0021] (2) Through the designed limiting member, the tissue sample is limited on all four sides and above by the long baffle, short baffle and baffle strip, effectively preventing the tissue from floating or moving within the glass slide, ensuring that the tissue maintains the correct position during the incubation process. The distance between the long baffle and the short baffle can be adjusted to accommodate tissues of different sizes. When the incubation device is transferred or accidentally collided, the limiting member can limit the tissue sample, reducing the impact of external shock on the tissue and avoiding tissue damage.

[0022] (3) Through the designed blocking mechanism composed of the plate frame, vertical block, vertical plate, movable frame, magnet plate and iron sheet, the movement of the plate frame can be driven by the rising and falling of the vertical block. The plate frame can effectively cover the primary antibody solution and tissue on the glass slide, preventing the primary antibody solution and tissue from splashing. Moreover, the threaded fit between the screw column and the vertical plate can precisely adjust the downward pressure of the cover glass, increasing the sealing performance, and can also press and limit the glass slide again. At the same time, after the glass slide is placed on the placement rack, the glass slide can be individually limited, avoiding the probability that the glass slide moves due to accidentally touching the glass slide when placing the tissue or dropping the primary antibody solution in the subsequent process. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 is a schematic structural diagram of the present invention;

[0024] Figure 2 is a schematic cross-sectional structural diagram of the box body of the present invention;

[0025] Figure 3 is a schematic rear view structural diagram of the placement rack of the present invention;

[0026] Figure 4 is a schematic bottom view structural diagram of the glass slide of the present invention;

[0027] Figure 5 is a schematic connection structural diagram of the cover plate and the connecting plate of the present invention;

[0028] Figure 6 is a schematic structural diagram of the vertical bar and the front frame of the present invention;

[0029] Figure 7 is a schematic meshing structural diagram of the worm gear and the worm of the present invention;

[0030] Figure 8 is a schematic structural diagram of the cover plate in an erected state of the present invention;

[0031] Figure 9 is a schematic structural diagram of the limiting member of the present invention;

[0032] Figure 10 is a schematic structural diagram of the long baffle and the short baffle of the present invention;

[0033] Figure 11Schematic diagram of the vertical block and vertical plate structure of the present invention;

[0034] Figure 12 Schematic diagram of the cross-sectional structure of the slide of the present invention;

[0035] Figure 13 Schematic diagram of the movable frame structure of the present invention;

[0036] In the figure: 100, box body; 101, box cover; 102, placement rack; 103, slide; 105, tissue; 200, cover plate; 201, rear plate; 202, vertical strip; 203, sponge block; 204, front rack; 205, lifting screw rod; 206, limit rod; 207, rotating rod; 208, connecting plate; 209, hollow T-shaped rod; 210, front locking screw; 211, hollow block; 212, rotating rod; 214, hydrophobic film; 215, rubber plate; 218, support bearing; 219, worm; 220, worm gear; 221, rear locking screw; 300, long retaining rack; 301, short retaining rack; 303, retaining strip; 304, magnet; 305, iron plate; 306, medical-grade silica gel plate; 400, plate rack; 401, vertical block; 402, vertical plate; 406, movable frame; 407, magnet plate; 408, iron sheet. Detailed implementation manners

[0037] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without making creative efforts shall fall within the protection scope of the present invention.

[0038] Embodiment 1

[0039] Please refer to Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 and Figure 8, the present invention provides a technical solution: an incubation device for immunohistochemistry, including a box body 100, a box cover 101 located on the box body 100, multiple groups of glass slides 103 placed on a placement rack 102, and tissues 105 arranged on the tops of the glass slides 103. A blocking mechanism is arranged on the box body 100. The blocking mechanism includes a covering component. The covering component includes a cover plate 200 arranged above the glass slide 103. A rubber plate 215 is fixedly arranged at the bottom of the cover plate 200. Through the rubber plate 215, the hydrophobic film 214 can be moved closer to the glass slide 103, increasing the sealing performance between the hydrophobic film 214 and the glass slide 103. A hydrophobic film 214 is fixedly arranged at the bottom of the rubber plate 215 for covering the primary antibody solution and the tissue 105 placed on the glass slide 103;

[0040] Linking plates 208 are fixedly arranged between multiple groups of cover plates 200. A flipping member is arranged between the top of the cover plate 200 and the box body 100. Through the flipping member, it is possible to cover, block, and limit the solution and the tissue 105 placed on the glass slide 103. When the incubation is completed, it is in a vertical state, without affecting the normal operation of the operator to pick up and place the tissue 105. The flipping member includes multiple groups of vertical bars 202 and a rear plate 201 fixed to the inner wall of the box body 100. One end of the rear plate 201 and the vertical bar 202 are rotationally connected through a rotating shaft.

[0041] Embodiment Two

[0042] On the basis of Embodiment One, please refer to Figures 2 - 8 , the blocking mechanism further includes a lifting component for driving the glass slide 103 to move up and down;

[0043] The lifting component includes a lifting screw 205 and a limiting rod 206. The bottom of the lifting screw 205 is fixedly connected to the cover plate 200. A support bearing 218 is arranged on the vertical bar 202. A threaded sleeve is fixed to the inner wall of the inner ring in the support bearing 218. A threaded sleeve is rotatably arranged inside the support bearing 218. The lifting screw 205 is threadedly connected to the threaded sleeve. The limiting rod 206 is fixed to the top of the cover plate 200, and the top of the limiting rod 206 passes through the inside of the vertical bar 202. The limiting rod 206 can move up and down inside the vertical bar 202 during lifting;

[0044] A hollow block 211 is fixedly arranged on the vertical bar 202. A rotating rod 212 is rotatably arranged inside the hollow block 211. The top of the lifting screw 205 passes through the inside of the hollow block 211. A worm gear 220 is arranged outside the threaded sleeve. Two groups of worm shafts 219 are fixedly arranged outside the rotating rod 212. The worm shaft 219 and the worm gear 220 are meshed with each other;

[0045] At the other end of the vertical bar 202, a hollow T-shaped rod 209 is fixedly arranged. A rotating rod 207 is rotatably arranged inside the hollow T-shaped rod 209. The end of the rotating rod 212 penetrates into the inside of the hollow T-shaped rod 209, and a first bevel gear is fixedly arranged at the end of the rotating rod 212. A second bevel gear is fixedly arranged outside the rotating rod 207. The first bevel gear meshes with the second bevel gear;

[0046] A front frame 204 is fixedly arranged on multiple groups of hollow T-shaped rods 209. Through the front frame 204, multiple groups of hollow T-shaped rods 209 are connected and fixed together. The rotating rod 207 is rotatably arranged inside the front frame 204;

[0047] Multiple embedding grooves are formed at the top of the front surface of the box body 100. Two sponge blocks 203 are fixedly arranged on the inner wall of the embedding groove, and the rotating rod 212 can penetrate between the two sponge blocks 203. The sponge blocks 203 can increase the blocking effect between the rotating rod 212 and the embedding groove without affecting the normal use of the rotating rod 212;

[0048] A rear locking screw 221 is connected to the vertical bar 202 by a thread. After the working end of the rear locking screw 221 is screwed into the screw port, it can fix and limit the erected cover plate 200. The working end of the rear locking screw 221 extends into the screw port formed on the inner wall surface of the box body 100. A front locking screw 210 is connected to the front frame 204 by a thread. The front locking screw 210 can lock and fix the front frame 204 in a horizontal state after being turned 90 degrees to prevent it from moving during the subsequent transfer of the incubation box. The working end of the front locking screw 210 extends to the inner wall surface of the box body 100.

[0049] Through the designed blocking mechanism of the present invention, the blocking mechanism effectively covers the primary antibody solution and the tissue 105 on the glass slide 103 through the covering component. During the process of transferring the incubator into the refrigerator, if an accidental collision or fall occurs, the hydrophobic film 214 can utilize its hydrophobic property to make the splashed solution form water droplets on its surface and slide back into the placement groove of the glass slide 103, thus avoiding the waste of the solution and the pollution of the adjacent glass slides 103 and the box body 100. The cover plate 200 can be turned to a vertical state, which does not affect the operator's normal operation of taking and placing the tissue 105, improves the incubation efficiency, and can also press and limit the glass slide 103 placed on the placement rack 102 to prevent it from moving.

[0050] In summary, before dropping the primary antibody solution onto the glass slide 103, multiple cover plates 200 are in an upright state. After dropping the primary antibody solution onto the glass slide 103, first rotate the rear locking screw 221 in the forward direction of the box body 100, and then its working end disengages from the screw thread in the inner wall of the box body 100, releasing the fixation of the vertical bar 202. Then, rotate the front frame 204 by 90 degrees in the forward direction of the box body 100 with the rotation axis on the rear plate 201 as the center. At this time, structures such as the vertical bar 202, the lifting screw 205, the hollow T-shaped rod 209, and the cover plate 200 will follow the rotation. At this time, the end of the hollow T-shaped rod 209 penetrates into the middle of the two sponge blocks 203. At this time, the sponge blocks 203 are compressed under the extrusion of the hollow T-shaped rod 209, increasing the blocking effect between the hollow T-shaped rod 209 and the embedding groove, and preventing foreign objects from entering the box body 100 through the embedding groove. At this time, the cover plate 200 is located above the glass slide 103. Then, move the front locking screw 210 on the front frame 204 in the direction close to the rear of the box body 100. Then, the working end of the front locking screw 210 contacts and clamps the box body 100, locking and fixing the front frame 204. Subsequently, hold the rotating rod 207 and rotate it, driving the second bevel gear outside it to rotate. The second bevel gear drives the first bevel gear meshing with it to rotate, thereby driving the rotating rod 212 to rotate. The rotating rod 212 drives the externally fixed worm 219 to rotate, and then drives the meshing worm gear 220 to rotate. The worm gear 220 drives the threaded sleeve installed on the inner wall of the support bearing 218 to rotate. At this time, the lifting screw 205 starts to move downward under the action of the rotating threaded sleeve, thereby driving the cover plate 200 below the lifting screw 205 to move downward. At this time, the limiting rod 206 on the top of the cover plate 200 slides and moves downward on the vertical bar 202. At this time, the cover plate 200 drives the remaining multiple cover plates 200 to move downward through the connecting plate 208. When the cover plate 200 moves downward, it will drive the rubber plate 215 and the hydrophobic film 214 below to move. At this time, the hydrophobic film 214 contacts and covers the glass slide 103, providing a barrier protection for the primary antibody solution and the tissue 105 placed in the placement groove of the glass slide 103;

[0051] At this time, the box cover 101 is covered on the box body 100 and fixed, and then the incubator is placed in the refrigerator. If an accidental collision or drop occurs, when the primary antibody solution on the glass slide 103 splashes, it will be blocked by the hydrophobic film 214. Due to the hydrophobic property of the hydrophobic film 214, when the solution splashes onto the surface of the hydrophobic film 214, the contact angle of the solution on its surface is relatively large, so that the solution will not spread on the surface of the hydrophobic film 214, but will aggregate into solution droplets. Under the action of gravity, these droplets are likely to roll or slide on the surface of the hydrophobic film 214, and thus can quickly detach from the surface of the hydrophobic film 214 and fall into the placement groove in the glass slide 103, avoiding waste caused by the outward splash of the solution. At the same time, the cover plate 200 can also press and limit the glass slide 103 to prevent it from being displaced or dropped due to the impact of accidental collision or drop;

[0052] When the incubation is completed, take it out of the refrigerator, and then operate according to the reverse principle above to turn the cover plate 200 back to its original upright state.

[0053] Example Three

[0054] Based on Example Two, please refer to Figure 1 、 Figure 2 、 Figure 9 and Figure 10 Inside the glass slide 103, there is a placement groove, and the tissue 105 is located inside the placement groove. Inside the glass slide 103, there is a limiting member for limiting the tissue 105. The limiting member includes two groups of long retaining frames 300 and two groups of short retaining frames 301. On both sides of the inner wall of the placement groove, magnets 304 are fixedly arranged. At both ends of the long retaining frame 300, iron plates 305 are fixedly arranged, and the iron plates 305 and the magnets 304 are magnetically connected. A thin layer of epoxy resin coating is sprayed on the outside of both the magnets 304 and the iron plates 305. Epoxy resin is a non-magnetic material, which will not interfere with the magnetic field lines of the magnets 304 themselves and will not be magnetized. Moreover, the epoxy resin coating has good corrosion resistance and can resist the erosion of salts, buffers, etc. in the primary antibody solution to avoid the release of iron ions affecting the primary antibody solution;

[0055] On both ends of the short retaining frame 301, two groups of medical-grade silica gel plates 306 are fixedly arranged. The medical-grade silica gel plates 306 have strong chemical inertness, are resistant to acids, alkalis, and organic solvents, and are not easy to adsorb proteins or release chemical substances to interfere with antibody activity, and will not affect the primary antibody solution. On the short retaining frame 301, two groups of retaining bars 303 are rotatably arranged through damping rotating shafts, and the retaining bars 303 can limit the area above the tissue 105.

[0056] In this embodiment, the placement rack 102 is fixed to the inner wall of the box body 100. Side plates are fixedly arranged on both sides of the box cover 101. The side plates are fixedly connected to the box body 100 through bolts. The bolts can be used to disassemble and assemble between the side plates and the box body 100, and then the box cover 101 can be opened or closed.

[0057] In the present invention, through the designed limiting members, the tissue 105 sample is limited on the four sides and above by the long blocking rack 300, the short blocking rack 301 and the blocking strip 303, effectively preventing the tissue 105 from floating or moving in the glass slide 103, ensuring that the tissue 105 maintains the correct position during the incubation process. The distance between the long blocking rack 300 and the short blocking rack 301 can be adjusted to accommodate tissues 105 of different sizes. When the incubation device is transferred or accidentally collided, the limiting members can limit the tissue 105 sample, reducing the impact of external shock on the tissue 105 and avoiding damage to the tissue 105.

[0058] In summary, before placing the tissue 105 into the placement groove in the glass slide 103, limit it according to the size of the tissue 105 to avoid the tissue 105 on the glass slide 103 from floating and moving during transfer, collision or dropping. When limiting, take out the two groups of long blocking racks 300 and the two groups of short blocking racks 301 outward. At this time, the two groups of iron plates 305 on the long blocking rack 300 are no longer adsorbed to the magnets 304. Then, move the two groups of long blocking racks 300 in the opposite or same direction according to the size of the tissue 105 to adjust the distance between them, driving the iron plates 305 to move. After adjustment, put the two groups of long blocking racks 300 into the placement groove in the glass slide 103, and at the same time make the iron plates 305 at both ends of the long blocking rack 300 adsorbed to the magnets 304. Then, move the two groups of short blocking racks 301 in the opposite or same direction according to the size of the tissue 105 to adjust the distance, and then insert them into the corresponding grooves in the two groups of long blocking racks 300. At this time, the medical-grade silica gel plates 306 on the short blocking racks 301 are also inserted into the grooves. At this time, the medical-grade silica gel plates 306 are squeezed by the inner wall of the grooves in the long blocking racks 300, increasing the friction between them and the inner wall of the grooves. At this time, the short blocking racks 301 are clamped on the long blocking racks 300. Then, turn the blocking strip 303 outward to open, and then limit the tissue 105 on the four sides and above through the two groups of long blocking racks 300, the two groups of short blocking racks 301 and multiple groups of blocking strips 303, so that the tissue 105 is restricted at the corresponding position in the placement groove in the glass slide 103. When the incubation is completed, turn the blocking strip 303 to be arranged parallel to the short blocking rack 301, and then take out the incubated tissue 105. When limiting tissues 105 of other sizes, adjust the distance between the two groups of long blocking racks 300 and the two groups of short blocking racks 301 in the limiting members in the same way as above.

[0059] Embodiment Four

[0060] On the basis of Embodiment Three, please refer to Figure 11, Figure 12 and Figure 13 , the blocking mechanism includes a plate frame 400 arranged above the glass slide 103 and a movable frame 406 fixed to the top of the plate frame 400. A cover glass is fixedly arranged at the bottom of the plate frame 400, and a hydrophobic coating is sprayed on the bottom of the cover glass. When the solution splashes onto the hydrophobic coating, the solution forms water droplets and slides into the placement groove;

[0061] A vertical block 401 is fixedly arranged at the top of the movable frame 406, and the top of the vertical block 401 passes through the inside of the box cover 101. A vertical plate 402 is fixedly arranged at the top of the box cover 101. A screw column is connected inside the vertical plate 402 by a thread, and the working end of the screw column extends to the surface of the vertical block 401. Iron sheets 408 are fixedly arranged at the bottom ends of both ends of the glass slide 103, and limiting grooves are opened at the top ends of both ends of the placement rack 102. A magnet plate 407 is fixedly arranged at the bottom of the inner wall of the limiting groove. The iron sheet 408 and the magnet plate 407 are magnetically connected. The glass slide 103 placed on the placement rack 102 can be individually limited by magnetic adsorption connection.

[0062] The present invention is composed of a blocking mechanism designed by the plate frame 400, the vertical block 401, the vertical plate 402, the movable frame 406, the magnet plate 407 and the iron sheet 408. The movement of the plate frame 400 can be driven by the rising and falling of the vertical block 401. The primary antibody solution and the tissue 105 on the glass slide 103 can be effectively covered by the plate frame 400 to prevent the primary antibody solution and the tissue 105 from splashing. Moreover, the thread fit between the screw column and the vertical plate 402 can precisely adjust the downward pressure of the cover glass to increase the sealing performance. At the same time, after the glass slide 103 is placed on the placement rack 102, the glass slide 103 can be individually limited to avoid the probability that the glass slide 103 moves due to accidentally touching the glass slide 103 during the subsequent placement of the tissue 105 or dropping of the primary antibody solution.

[0063] In summary, after placing the sliced tissue 105 into the placement groove in the glass slide 103, a primary antibody solution is dropped into the placement groove in the glass slide 103 to cover the tissue 105. Then, the box cover 101 is covered on the box body 100. Subsequently, the screw column on the vertical plate 402 is rotated and loosened towards the front direction of the box body 100. Then, the working end of the screw column disengages from the surface of the vertical block 401, releasing the fixation of the vertical block 401. Then, the vertical block 401 is slowly moved downward, driving the movable frame 406 to move. The movable frame 406 drives multiple groups of plate frames 400 below to move. The plate frames 400 drive the cover glass below to move. Then, the bottom of the cover glass contacts and covers the glass slide 103, covering the placement groove on the glass slide 103. Finally, the screw column on the vertical plate 402 is rotated towards the back direction of the box body 100. Then, the working end of the screw column contacts and clamps the vertical block 401, fixing the vertical block 401. When the incubator is transferred or accidentally collides and drops to generate an impact, the cover glass limits and blocks the solution and the tissue 105 located in the placement groove, preventing the solution from splashing. When the incubation is completed, the operation can be carried out according to the reverse principle described above;

[0064] When the glass slide 103 is placed on the placement rack 102 before incubation, the iron sheet 408 at the bottom of the glass slide 103 is inserted into the corresponding limiting groove at the top of the placement rack 102 and contacts and adsorbs with the magnet plate 407, which can limit the position of the glass slide 103, facilitating the operation and preventing the glass slide 103 from moving accidentally when the tissue 105 is placed into the placement groove in the glass slide 103 or when the primary antibody solution is dropped into the placement groove.

[0065] The above embodiments are only used to illustrate the technical method of the present invention and not to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical method of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical method of the present invention.

Claims

1. An incubation device for immunohistochemistry, comprising a box body (100), a box cover (101) located on the box body (100), multiple groups of glass slides (103) placed on a placement rack (102), and tissues (105) arranged on the tops of the glass slides (103), wherein a blocking mechanism is arranged on the box body (100), and it is characterized in that, The blocking mechanism includes: A covering component, including a cover plate (200) arranged above the glass slide (103). A rubber plate (215) is fixedly arranged at the bottom of the cover plate (200), and a hydrophobic film (214) is fixedly arranged at the bottom of the rubber plate (215), for covering the primary antibody solution and the tissue (105) placed on the glass slide (103); A connecting plate (208) is fixedly arranged between multiple groups of the cover plates (200). A turning piece is arranged between the top of the cover plate (200) and the box body (100). The turning piece includes multiple groups of vertical bars (202) and a rear plate (201) fixed to the inner wall of the box body (100).

2. The incubation device for immunohistochemistry according to claim 1, wherein: The blocking mechanism further includes a lifting component for driving the glass slide (103) to move up and down; The lifting component includes a lifting screw rod (205) and a limiting rod (206). A support bearing (218) is arranged on the vertical bar (202), and a threaded sleeve is rotatably arranged inside the support bearing (218).

3. The incubation device for immunohistochemistry according to claim 2, wherein: A hollow block (211) is fixedly arranged on the vertical bar (202), and a rotating rod (212) is rotatably arranged inside the hollow block (211). A worm gear (220) is arranged outside the threaded sleeve, and two groups of worm shafts (219) are fixedly arranged outside the rotating rod (212).

4. An incubation device for immunohistochemistry according to claim 1, wherein: The other end of the vertical bar (202) is fixedly provided with a hollow T-shaped rod (209), and a rotating rod (207) is rotatably arranged inside the hollow T-shaped rod (209).

5. The incubation device for immunohistochemistry according to claim 4, wherein: Multiple groups of hollow T-shaped rods (209) are fixedly provided with a front frame (204). Multiple groups of embedding grooves are opened at the top of the front surface of the box body (100), and two groups of sponge blocks (203) are fixedly arranged on the inner walls of the embedding grooves; A rear locking screw rod (221) is connected to the vertical bar (202) by a thread, and a front locking screw rod (210) is connected to the front frame (204) by a thread.

6. The incubation device for immunohistochemistry according to claim 1, wherein: A placement groove is opened inside the glass slide (103), and a limiting piece for limiting the tissue (105) is arranged inside the glass slide (103). The limiting piece includes two groups of long blocking frames (300) and two groups of short blocking frames (301). Magnets (304) are fixedly arranged on both sides of the inner wall of the placement groove, and iron plates (305) are fixedly arranged at both ends of the long blocking frame (300).

7. The incubation device for immunohistochemistry according to claim 6, characterized in that: Two groups of medical-grade silica gel plates (306) are fixedly arranged on both surfaces of both ends of the short blocking frame (301), and two groups of blocking bars (303) are rotatably arranged on the short blocking frame (301) through damping rotating shafts.

8. An incubation device for immunohistochemistry according to claim 1, characterized in that: The blocking mechanism includes a plate frame (400) arranged above the glass slide (103) and a movable frame (406) fixed to the top of the plate frame (400). A cover glass is fixedly arranged at the bottom of the plate frame (400).

9. An incubation device for immunohistochemistry according to claim 8, characterized in that: A vertical block (401) is fixedly arranged at the top of the movable frame (406), a vertical plate (402) is fixedly arranged at the top of the box cover (101), and a spiral column is connected to the inside of the vertical plate (402) by a thread.

10. The incubation device for immunohistochemistry according to claim 1, characterized in that: The placement rack (102) is fixed to the inner wall of the box body (100), and side plates are fixedly arranged on both sides of the box cover (101).

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