Cell sorting equipment

Abstract

The invention discloses cell sorting equipment, and relates to the technical field of cell sorting. The device comprises a box body, an upper cover is installed on the box body, a cylinder is sleeved with the interior of the upper cover in a limiting mode, a lifting polygonal column is movably sleeved with the interior of the cylinder, and an empty box is fixed to the upper end of the polygonal column. The needle tube penetrates through the bottom opening to be inserted into the rubber cover, then the water pumping mechanism or the water conveying mechanism is started, so that the water pumping mechanism pumps upper-layer cell clear liquid in the test tube through the empty box, the side opening and the needle tube, and after extraction, the upper-layer cell clear liquid is guided into the empty box through the water conveying mechanism; the upper-layer cell clear liquid flows into the empty test tube through the side opening and the needle tube to be centrifugally rotated, and the operation is repeated, so that the equipment can take out the centrifugally rotated upper-layer cell clear liquid without shutdown and place the centrifugally rotated upper-layer cell clear liquid into the empty test tube to be centrifugally rotated, and the use effect of the equipment is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of cell sorting, and particularly relates to a cell sorting device. Background Art

[0002] The function of cell sorting is to separate specific cell subsets from a mixed cell sample for subsequent biochemical analysis and functional research. Cell sorting technology analyzes the characteristics of cells to ensure obtaining target cells with high purity, thus providing a reliable premise and basis for cell function research.

[0003] In a Chinese patent (application number: CN202320818955.2), a device for preparing cell sorting nano-magnetic bead reagents is disclosed, which includes a preparation box. Inside the preparation box, a reagent disk is rotatably installed. A number of reagent grooves are evenly distributed inside the reagent disk. An activity plate is rotatably installed inside the preparation box. A rotating plate is rotatably installed inside the preparation box. One end of the rotating plate is fixedly installed with a rotating block, and the rotating block is in movable contact with the inner wall of the activity plate. The central part of the bottom of the reagent disk is fixedly installed with a connecting column. The bottom of the connecting column is fixedly connected with an arc plate. The bottom of the end of the arc plate away from the connecting column is fixedly connected with a cylinder, and the cylinder is in movable contact with the inner wall of the activity plate. In the present invention, the rotating plate drives the rotating block to rotate inside the activity plate, causing the activity plate to perform a sector motion. The activity plate drives the arc plate to perform a sector motion simultaneously through the cylinder, so that the connecting column drives the reagent disk to rotate back and forth, and the reagent tubes inside the reagent grooves are fully mixed and stirred.

[0004] The following technical problems exist in the actual use of this patent and the prior art:

[0005] During the existing cell centrifugation sorting, it is necessary to repeatedly take out the supernatant cell sample after centrifugation and then put it into the centrifugation sorting device for centrifugation sorting. As a result, the operator needs to often stop the centrifugation sorting device and then start the centrifugation sorting device again. Such a sorting method not only increases the workload of the operator but also reduces the use effect of the device. Therefore, it needs to be improved. Summary of the Invention

[0006] The purpose of the present invention is: to solve the above problems, the present invention provides a cell sorting device.

[0007] The present invention specifically adopts the following technical solutions to achieve the above purpose:

[0008] A cell sorting device comprises a box body, on which an upper cover is installed, a cylinder is sleeved inside the upper cover, a lifting polygonal column is movably sleeved inside the cylinder, an empty box is fixed on the upper end of the polygonal column, a collar block is sleeved inside the empty box, a water delivery mechanism and a water pumping mechanism are fixedly connected at both ends of the collar block, a needle tube is movably sleeved inside the empty box, a closing block for closing the empty box is fixed on the top of the needle tube, a bottom opening below the needle tube is provided inside the cylinder, a side opening is provided inside the needle tube, a centrifugally rotating socket disk is installed inside the box body, test tubes are equidistantly placed in a ring inside the socket disk, a rubber cover below the needle tube is installed on the upper end of the test tube, and a polygonal hole below the polygonal column is provided inside the socket disk.

[0009] Furthermore, a driving motor is fixedly installed inside the box, and the upper end of the driving motor is transmission-connected with a rotating rod, and the upper end of the rotating rod penetrates the box and is fixedly connected to the bottom of the jack plate.

[0010] Furthermore, a circular ring is fixedly sleeved on the outer surface of the needle tube, a rigid spring is fixedly connected to the top of the circular ring, and an upper end of the rigid spring is fixedly connected to the bottom of the empty box.

[0011] Furthermore, the pumping mechanism includes a second fixed block fixed to the side of the upper cover, a second water pump is fixedly installed on the bottom of the second fixed block, the upper end of the second water pump is fixedly connected to a second hose, the other end of the second hose passes through the second fixed block and the upper cover in sequence and is fixedly connected to the collar block, and the left end of the second water pump is fixedly connected to a second fixed pipe.

[0012] Furthermore, the water delivery mechanism includes a first fixed block fixed to the side of the upper cover, a first water pump is fixedly installed on the side of the first fixed block, the upper end of the first water pump is fixedly connected to a first hose, the other end of the first hose passes through the upper cover and is fixedly connected to the collar block, and the side of the first water pump is fixedly connected to a first fixed pipe.

[0013] Furthermore, an electric telescopic rod is fixedly mounted on the upper end of the upper cover, the lower end of the electric telescopic rod is limitedly sleeved with the polygonal column, and a controller electrically connected to the electric telescopic rod is installed inside the box.

[0014] Furthermore, a controller is installed inside the box, and the controller is electrically connected to the electric telescopic rod.

[0015] Furthermore, a detection camera for detecting the test tube is installed inside the box, and the detection camera is electrically connected to the controller.

[0016] The beneficial effects of the present invention are as follows:

[0017] 1. In the present invention, the syringe is inserted through the bottom opening into the inside of the rubber cap, and then by starting the water pumping mechanism or the water conveying mechanism, the water pumping mechanism extracts the upper-layer cell supernatant in the test tube through the empty box and the side opening and the syringe. After extraction, the upper-layer cell supernatant is introduced into the empty box through the water conveying mechanism, so that the upper-layer cell supernatant flows into the empty test tube through the side opening and the syringe for centrifugal rotation. By repeating such operations, the device can take out the upper-layer cell supernatant after centrifugal rotation without stopping the machine and put it into the empty test tube for centrifugal rotation, thereby improving the use effect of the device.

[0018] 2. In the present invention, the multi-sided column is inserted into the multi-sided hole, so that the rotating jack plate drives the empty box and the cylinder to rotate through the multi-sided hole and the multi-sided column, so that the empty box drives the syringe to insert through the bottom opening into the inside of the rubber cap, and further the rubber cap pushes the side opening on the syringe to move into the inner cavity of the empty box until the syringe is inserted into the upper-layer cell supernatant inside the test tube. Then, by starting the water conveying mechanism, the cell stock solution sample is introduced into the empty box, so that the cell stock solution sample flows into the empty test tube through the side opening and the syringe, thereby achieving the purpose of centrifugal sorting of adding the cell stock solution sample without stopping the machine.

[0019] 3. In the present invention, the detection camera detects the cell stock solution sample inside the test tube, and the detection camera transmits the detection data to the controller, so that the controller controls the electric telescopic rod to start, and further the electric telescopic rod drives the syringe to insert through the bottom opening into the inside of the test tube, thereby achieving the purpose of timely extracting the upper-layer cell supernatant sample inside the test tube, so as to improve the efficiency of sorting cell samples by the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is a schematic diagram of the present invention;

[0021] Figure 2 is a schematic diagram of the back of the present invention;

[0022] Figure 3 is a front cross-sectional schematic diagram of the present invention;

[0023] Figure 4 is a side cross-sectional schematic diagram of the box body of the present invention;

[0024] Figure 5 is a schematic diagram of the syringe and the rubber cap of the present invention;

[0025] Figure 6 is the present invention Figure 1 partial enlarged schematic diagram at A;

[0026] Figure 7 is the present invention Figure 2 partial enlarged schematic diagram at B;

[0027] Figure 8 is the present inventionFigure 3 Partial enlarged schematic view at position C

[0028] Reference numerals: 1, box body; 2, upper cover; 3, cylinder; 4, multi-sided column; 5, empty box; 6, closing block; 7, syringe; 8, ring; 9, rigid spring; 10, side port; 11, bottom port; 12, jack plate; 13, test tube; 14, rubber cap; 15, water delivery mechanism; 1501, first fixing block; 1502, first water pump; 1503, first hose; 1504, first fixing tube; 16, water pumping mechanism; 1601, second fixing block; 1602, second water pump; 1603, second hose; 1604, second fixing tube; 17, electric telescopic rod; 18, detection camera; 19, controller; 20, drive motor; 21, rotating rod; 22, multi-sided hole; 23, collar block Detailed implementation manners

[0029] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention

[0030] Embodiment 1, as Figures 1 - 8 shown, a cell sorting device includes a box body 1, an upper cover 2 is installed on the box body 1, a cylinder 3 is limitedly sleeved inside the upper cover 2, a multi-sided column 4 that can be lifted is movably sleeved inside the cylinder 3, an empty box 5 is fixed at the upper end of the multi-sided column 4, a collar block 23 is limitedly sleeved inside the empty box 5, a water delivery mechanism 15 and a water pumping mechanism 16 are respectively fixedly communicated at both ends of the collar block 23, a syringe 7 is movably sleeved inside the empty box 5, a closing block 6 that closes the empty box 5 is fixed at the top of the syringe 7, a bottom port 11 located below the syringe 7 is opened inside the cylinder 3, a side port 10 is opened inside the syringe 7, a jack plate 12 that rotates centrifugally is installed inside the box body 1, test tubes 13 are annularly and equidistantly placed inside the jack plate 12, a rubber cap 14 located below the syringe 7 is installed at the upper end of the test tube 13, and a multi-sided hole 22 located below the multi-sided column 4 is opened inside the jack plate 12

[0031] When it is necessary to take out the supernatant of the cells inside the test tube 13 after centrifugal rotation, by moving the multi-sided column 4 into the multi-sided hole 22, the multi-sided column 4 drives the empty box 5 to move downward. As a result, the rotating socket plate 12 can drive the empty box 5 to rotate through the multi-sided hole 22 and the multi-sided column 4. At the same time, the multi-sided column 4 drives the cylinder 3 to rotate synchronously along the inner wall of the upper cover 2. As the multi-sided column 4 continues to move downward, the syringe 7 inside the empty box 5 will pass through the bottom port 11 and insert into the rubber cap 14. At this time, due to the friction between the syringe 7 and the rubber cap 14, the rubber cap 14 squeezes the sealing block 6 onto the inner wall of the upper end of the empty box 5 through the syringe 7. At the same time, the side port 10 on the syringe 7 moves into the inner cavity of the empty box 5. Then, when the syringe 7 is inserted into the supernatant of the cells inside the test tube 13, by starting the water pumping mechanism 16, the water pumping mechanism 16 extracts the supernatant of the cells inside the test tube 13 through the empty box 5, the side port 10 and the syringe 7. After extraction, move the multi-sided column 4 upward, so that the multi-sided column 4 disengages from the inside of the multi-sided hole 22. At the same time, the syringe 7 and the rubber cap 14 will pull the sealing block 6 to seal the empty box 5 through friction until the syringe 7 disengages from the rubber cap 14. Then, the supernatant of the cells is introduced into the empty box 5 through the water conveying mechanism 15. Subsequently, by moving the multi-sided column 4 downward again, the empty box 5 drives the syringe 7 to insert into an empty test tube 13, so that the supernatant of the cells inside the empty box 5 flows into the empty test tube 13 through the side port 10 and the syringe 7 for centrifugal rotation. By repeating such operations, the device can take out the supernatant of the cells after centrifugal rotation without stopping the machine and put it into an empty test tube 13 for centrifugal rotation, thereby improving the use effect of the device.

[0032] Further, a driving motor 20 is fixedly installed inside the box body 1. The upper end of the driving motor 20 is drivingly connected with a rotating rod 21. The upper end of the rotating rod 21 penetrates through the box body 1 and is fixedly connected to the bottom of the socket plate 12.

[0033] By starting the driving motor 20, the rotating rod 21 drives the socket plate 12 to rotate along the inner wall of the box body 1. As a result, the socket plate 12 drives the test tube 13 to rotate, so that the cell samples inside the test tube 13 can be centrifugally sorted, thus achieving the purpose of centrifugally sorting the cell samples by the rotating socket plate 12.

[0034] Further, a circular ring 8 is fixedly sleeved on the outer surface of the syringe 7. The top of the circular ring 8 is fixedly connected with a rigid spring 9. The upper end of the rigid spring 9 is fixedly connected to the bottom of the empty box 5.

[0035] When the syringe 7 is completely detached from the inside of the rubber cap 14, due to the design of the ring 8 and the rigid spring 9, the rigid spring 9 can squeeze the syringe 7 downward through the ring 8, so that the syringe 7 drives the sealing block 6 to fit more closely to the inner wall of the empty box 5, making it difficult for the liquid on the inner wall of the empty box 5 to flow out.

[0036] Embodiment 2, as Figures 3 - 7 shown, the water pumping mechanism 16 includes a second fixing block 1601 fixed to the side of the upper cover 2. A second water pump 1602 is fixedly installed at the bottom of the second fixing block 1601. The upper end of the second water pump 1602 is fixedly connected to a second hose 1603. The other end of the second hose 1603 sequentially penetrates the second fixing block 1601 and the upper cover 2 and is fixedly connected to the collar block 23. The left end of the second water pump 1602 is fixedly connected to a second fixing pipe 1604.

[0037] When the syringe 7 is inserted into the test tube 13, by starting the second water pump 1602 at this time, the second hose 1603 can extract the upper-layer cell supernatant sample inside the test tube 13 through the empty box 5 and the side port 10 and discharge the sample to the external test cup through the second fixing pipe 1604.

[0038] The water delivery mechanism 15 includes a first fixing block 1501 fixed to the side of the upper cover 2. A first water pump 1502 is fixedly installed on the side of the first fixing block 1501. The upper end of the first water pump 1502 is fixedly connected to a first hose 1503. The other end of the first hose 1503 penetrates the upper cover 2 and is fixedly connected to the collar block 23. The side of the first water pump 1502 is fixedly connected to a first fixing pipe 1504.

[0039] When it is necessary to introduce the upper cell supernatant sample or cell sample in the detection cup into the empty test tube 13, at this time, by moving the multi-sided column 4 downward and inserting it into the multi-sided hole 22, the rotating jack disk 12 will drive the empty box 5 and the cylinder 3 to rotate through the multi-sided hole 22 and the multi-sided column 4. As the multi-sided column 4 continues to move downward, the syringe 7 inside the empty box 5 will pass through the bottom opening 11 and insert into the rubber cover 14. At this time, the rubber cover 14 will squeeze the sealing block 6 onto the inner wall of the upper end of the empty box 5 through the syringe 7, and the side port 10 on the syringe 7 will move into the inner cavity of the empty box 5. As the multi-sided column 4 continues to move downward, the syringe 7 is inserted into the upper cell supernatant in the test tube 13. Then, by starting the first water pump 1502, the first fixed tube 1504 can extract the cell supernatant sample or cell stock solution sample in the detection cup, and then the first hose 1503 will introduce the upper cell supernatant sample or cell stock solution sample into the empty box 5, so that the upper cell supernatant sample or cell stock solution sample flows into the empty test tube 13 or the test tube 13 with sediment through the side port 10 and the syringe 7. In this way, the upper cell supernatant sample and the cell stock solution sample can be separated by centrifugation and sorting, thus achieving the purpose of adding cell stock solution sample for centrifugation and sorting without stopping the machine.

[0040] Embodiment 3, as Figure 1 and Figure 3 and Figure 4 shown, an electric telescopic rod 17 is fixedly installed at the upper end of the upper cover 2, the lower end of the electric telescopic rod 17 is sleeved with the multi-sided column 4 in a limiting manner, and a controller 19 electrically connected to the electric telescopic rod 17 is installed inside the box body 1.

[0041] By starting the electric telescopic rod 17, the electric telescopic rod 17 drives the multi-sided column 4 to move downward, and then the multi-sided column 4 can drive the syringe 7 to insert into the test tube 13 through the empty box 5 to extract the upper cell supernatant sample.

[0042] A controller 19 is installed inside the box body 1, and the controller 19 is electrically connected to the electric telescopic rod 17.

[0043] Through the design of the controller 19 and the electric telescopic rod 17, the controller 19 can control the electric telescopic rod 17 to start, and then the electric telescopic rod 17 drives the multi-sided column 4 to move upward or downward.

[0044] A detection camera 18 for detecting the test tube 13 is installed inside the box body 1, and the detection camera 18 is electrically connected to the controller 19.

[0045] When the detection camera 18 detects that the cell stock solution sample in the test tube 13 is centrifuged into the upper cell clear layer and the sediment, the detection camera 18 can transmit the detection signal to the controller 19, so that the controller 19 controls the electric telescopic rod 17 to start, so that the electric telescopic rod 17 drives the polygonal column 4 to move downward and insert into the polygonal hole 22. At this time, the drive motor 20 drives the jack plate 12 to rotate through the rotating rod 21, so that the jack plate 12 drives the empty box 5 and the cylinder 3 to rotate through the polygonal hole 22 and the polygonal column 4, and then the polygonal column 4 drives the empty box 5 and the cylinder 3 to rotate through the empty box 5. The needle tube 7 is inserted into the rubber cover 14 through the bottom opening 11, so that the rubber cover 14 pushes the sealing block 6 to move upward through the needle tube 7, and the side opening 10 on the needle tube 7 moves into the inner cavity of the empty box 5 until the needle tube 7 is inserted into the upper cell clear night inside the test tube 13. At this time, by starting the second water pump 1602, the second hose 1603 can extract the upper cell clear night sample inside the test tube 13 through the empty box 5 and the side opening 10 and the needle tube 7, and discharge the sample into the external detection cup through the second fixed tube 1604. When the detection camera 18 detects the upper cell clear night inside the test tube 13, After the sample is extracted, the detection signal is transmitted to the controller 19, so that the controller 19 controls the electric telescopic rod 17 to start, so that the electric telescopic rod 17 drives the polygonal column 4 upward, so that the empty box 5 drives the needle tube 7 to be separated from the rubber cover 14 through the closing block 6, and then the controller 19 detects the position of the empty test tube 13 in the jack plate 12 through the detection camera 18, so as to control the polygonal column 4 to insert into the polygonal hole 22, at which time the needle tube 7 will be located above the empty test tube 13, so that the needle tube 7 can be inserted into the empty test tube 13 through the rubber cover 14, and then the operator will detect The measuring cup is placed under the first fixed tube 1504, and the first fixed tube 1504 is allowed to extract the liquid in the measuring cup. Then, by starting the first water pump 1502, the first fixed tube 1504 extracts the upper cell clear sample in the measuring cup, and the first hose 1503 guides the upper cell clear sample into the empty box 5, so that the upper cell clear sample flows into the empty test tube 13 through the side port 10 and the needle tube 7 for centrifugal sorting, thereby achieving the purpose of timely extracting the upper cell clear sample in the test tube 13, thereby improving the efficiency of the device in sorting cell samples.

[0046] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A cell sorting device, comprising a box body (1), characterized in that, The box body (1) is provided with an upper cover (2), the inner limit sleeve of the upper cover (2) is connected with a cylinder (3), the inner part of the cylinder (3) is movably connected with a lifting polygonal column (4), the upper end of the polygonal column (4) is fixed with an empty box (5), the inner limit sleeve of the empty box (5) is connected with a collar block (23), the two ends of the collar block (23) are respectively fixedly connected with a water delivery mechanism (15) and a water pumping mechanism (16), the inner part of the empty box (5) is movably connected with a needle tube (7), and the top of the needle tube (7) is fixed with a sealing member. The sealing block (6) of the empty box (5) is provided. The cylinder (3) is provided with a bottom opening (11) located below the needle tube (7). The needle tube (7) is provided with a side opening (10). The box body (1) is provided with a centrifugally rotating socket disk (12). Test tubes (13) are placed in an annular manner at equal intervals inside the socket disk (12). The upper end of the test tube (13) is provided with a rubber cover (14) located below the needle tube (7). The socket disk (12) is provided with a polygonal hole (22) located below the polygonal column (4).

2. The cell sorting device according to claim 1, wherein, A driving motor (20) is fixedly installed inside the box (1), and the upper end of the driving motor (20) is drivingly connected to a rotating rod (21), and the upper end of the rotating rod (21) passes through the box (1) and is fixedly connected to the bottom of the socket plate (12).

3. The cell sorting device according to claim 1, wherein, A circular ring (8) is fixedly sleeved on the outer surface of the needle tube (7), a rigid spring (9) is fixedly connected to the top of the circular ring (8), and the upper end of the rigid spring (9) is fixedly connected to the bottom of the empty box (5).

4. The cell sorting device according to claim 1, wherein, The pumping mechanism (16) comprises a second fixed block (1601) fixed to the side of the upper cover (2); a second water pump (1602) is fixedly installed at the bottom of the second fixed block (1601); the upper end of the second water pump (1602) is fixedly connected to a second hose (1603); the other end of the second hose (1603) passes through the second fixed block (1601) and the upper cover (2) in sequence and is fixedly connected to the collar block (23); the left end of the second water pump (1602) is fixedly connected to a second fixed pipe (1604).

5. The cell sorting device according to claim 1, wherein, The water delivery mechanism (15) comprises a first fixed block (1501) fixed to the side of the upper cover (2); a first water pump (1502) is fixedly installed on the side of the first fixed block (1501); the upper end of the first water pump (1502) is fixedly connected to a first hose (1503); the other end of the first hose (1503) passes through the upper cover (2) and is fixedly connected to the collar block (23); and the side of the first water pump (1502) is fixedly connected to a first fixed pipe (1504).

6. The cell sorting device according to claim 1, wherein An electric telescopic rod (17) is fixedly mounted on the upper end of the upper cover (2), the lower end of the electric telescopic rod (17) is limitedly sleeved with the polygonal column (4), and a controller (19) electrically connected to the electric telescopic rod (17) is mounted inside the box body (1).

7. A cell sorting device according to claim 6, characterized in that, A controller (19) is installed inside the box (1), and the controller (19) is electrically connected to the electric telescopic rod (17).

8. A cell sorting device according to claim 7, wherein Inside the box body (1), a detection camera (18) for detecting test tubes (13) is installed, and the detection camera (18) is electrically connected to the controller (19).

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