Separation device for stem cells and use method

Abstract

The invention belongs to the technical field of stem cell treatment, particularly relates to a separation device for stem cells and a use method, and aims to solve the problems that a filter plate is arranged in a separation bin and is not easy to clean, the filter plate is easy to block due to long-time filtering and the filtering effect is reduced. The same partition plate is fixedly connected to the opposite sides of the interior of the separation bin, a filtering module is arranged on one side of the partition plate and comprises a screening treatment frame, one side of the screening treatment frame is fixedly connected with one side of the partition plate, and first round holes are formed in the two sides of the screening treatment frame and the two sides of the separation bin correspondingly; the interiors of the two first round holes located in the same side are connected with the same hollow rotating round pipe through bearings. According to the separation device for the stem cells and the use method, the cleaning effect is improved while the filter plate is cleaned, the situation that the filter plate is easily blocked due to long-time filtering is avoided, and therefore the filtering effect is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell processing, and in particular to a separation device for stem cells and a usage method thereof. Background Art

[0002] Stem cells are a type of cells with pluripotent differentiation potential. According to different sources, they can be divided into embryonic stem cells and adult stem cells. Embryonic stem cells can differentiate into any type of cell in the body, so they are also called totipotent stem cells. Adult stem cells refer to those stem cells with tissue or organ specificity, which can differentiate into cell lines suitable for their location in a suitable environment, and are also called multipotent stem cells. Stem cell beauty is a technology that effectively removes wrinkles and scars through the body's own skin stem cells. Its technological process is to extract a small amount of stem cells from the body for culture, and then transfer enough stem cells back into the body. Separation operation is required first when extracting stem cells.

[0003] When separating stem cells, it is usually necessary to filter the cell suspension, mainly used to remove bone fragments and cell clusters to ensure the purity of the obtained cell suspension. Since the filter plate is arranged inside the separation chamber, it is not easy to clean the filter plate. Long-term filtration easily causes the filter plate to become blocked, reducing the filtration effect. Summary of the Invention

[0004] The present invention discloses a separation device for stem cells and a usage method thereof, aiming to solve the technical problem that the filter plate in the background art is arranged inside the separation chamber, it is not easy to clean the filter plate, long-term filtration easily causes the filter plate to become blocked, reducing the filtration effect.

[0005] A separation device for stem cells proposed by the present invention includes a separation chamber. On opposite sides inside the separation chamber, there is a fixed connection of the same partition board, and a filtration module is arranged on one side of the partition board. The filtration module includes a sieving treatment frame, and one side of the sieving treatment frame is fixedly connected to one side of the partition board. Circular holes I are opened on both sides of the sieving treatment frame and the separation chamber, and inside the two circular holes I on the same side, there is a fixed connection of the same hollow rotating circular tube through bearings. On one side of the two hollow rotating circular tubes, there are notch openings. Inside the two notch openings, there are sliding connections of push-pull clamping columns. On the opposite sides of the two push-pull clamping columns, there is a fixed connection of the same sieving cylinder, and on the outer surface of the sieving cylinder, there are equidistant sieving holes. On the outer side of one side of the separation chamber, there is a fixed connection of a U-shaped limiting seat, and on one side of the U-shaped limiting seat, there is a circular opening. Inside the circular opening, there is a fixed connection of a feed pipe. On one side of one of the push-pull clamping columns, there is a feed port, and the feed pipe is movably arranged inside the feed port.

[0006] In a preferred embodiment, two perforations are formed on one side of the sieving and processing frame, and guiding round rods are slidably connected to the interiors of the two perforations. One ends of the two guiding round rods located inside the sieving and processing frame are fixedly connected to the same lifting support plate. Two smooth holes are formed in the lifting support plate, and adapting jack posts are slidably connected to the interiors of the two smooth holes. One ends of the two adapting jack posts are fixedly connected to the same cleaning brush plate. A same telescopic spring is fixedly connected to the top of the lifting support plate and one side of the two adapting jack posts. The telescopic spring surrounds the outside of the adapting jack post. An electric telescopic rod is fixedly connected to one side of the sieving and processing frame, and the driving end of the electric telescopic rod is fixedly connected to one side of the lifting support plate.

[0007] In a preferred embodiment, a guiding track is fixedly connected to one side inside the separation bin, and an adjusting rack is slidably connected to the interior of the guiding track. A support block is fixedly connected to one side of the adjusting rack. A rotating gear is fixedly connected to the outside of one of the hollow rotating round tubes. The rotating gear meshes with the adjusting rack. An electric driving rod is fixedly connected to one side inside the separation bin, and the driving end of the electric driving rod is fixedly connected to one side of the support block.

[0008] In a preferred embodiment, a discharge hole is formed on one side of the partition board, and a conveying pipe is fixedly connected to the interior of the discharge hole. One end of the conveying pipe is fixedly connected to a conveying pump. An electronic feeding pipe is arranged at the discharge end of the conveying pipe. A tooling circular long frame is fixedly connected to one side of one of the hollow rotating round tubes, and two openings are formed in the outside of the tooling circular long frame.

[0009] In a preferred embodiment, a linkage rack is fixedly connected to one side of one of the push-pull latch columns, and a slider is fixedly connected to one side of the linkage rack. The slider slides inside the tooling circular long frame. A same reset spring is fixedly connected to the opposite sides of the slider and the tooling circular long frame. Two fixing rods are fixedly connected to the outside of the tooling circular long frame, and two circular holes two are formed in one side of each of the two fixing rods. The interiors of the two opposite circular holes two are connected by bearings to the same rotating shaft.

[0010] In a preferred embodiment, one ends of the two rotating shafts are fixedly connected to linkage gears, and the two linkage gears mesh with each other. Semicircular gears are fixedly connected to the outside of the two rotating shafts. The tooth block ends of the semicircular gears mesh with the linkage rack. A driving motor is arranged on one side of one of the fixing rods, and the driving end of the driving motor is connected to one side of one of the rotating shafts through a coupling.

[0011] In a preferred embodiment, two limiting tracks are fixedly connected to one side inside the separation bin, and horizontal moving seats are slidably connected inside both of the two limiting tracks. A same blanking module is arranged on the two horizontal moving seats. The blanking module includes a moving support plate. A circular groove is formed on one side of the moving support plate, and a rotating sliding seat is slidably connected inside the circular groove. An adjusting and separating circular seat is arranged above the rotating sliding seat.

[0012] In a preferred embodiment, placing seats are movably connected to the adjusting and separating circular seat at equal intervals, and reagent tube bodies are placed inside multiple placing seats. Multiple pressing springs are annularly and equidistantly distributed inside the multiple placing seats. One side of the multiple pressing springs located on the same side is fixedly connected to a same U-shaped mounting plate. Arc-shaped rubber rollers are movably connected to the multiple U-shaped mounting plates at equal intervals. A universal motor is arranged at the bottom of the moving support plate, and the driving end of the universal motor is fixedly connected to one side of the adjusting and separating circular seat. An electric cylinder is arranged on one side of the separation bin.

[0013] In a preferred embodiment, a tooling cover plate is connected to one side of the separation bin by bolts, and a heating panel is arranged on the inner side of the tooling cover plate facing the inside of the separation bin. A temperature control panel is arranged on the outer side of the separation bin. An observation port is formed on one side of the separation bin, and an observation and access panel is connected to one side of the observation port by a hinge.

[0014] A usage method of a separation device for stem cells, using the separation device for stem cells as described above, includes the following steps:

[0015] Step 1: When filtering the cell suspension, first input the cell suspension into the sieve cylinder through the feed pipe, and then sieve the cell suspension through the sieve cylinder to leave the solid matter in the cell suspension inside the sieve cylinder.

[0016] Step 2: While filtering, start the electric telescopic rod. The electric telescopic rod drives the cleaning brush plate on the lifting support plate to abut against the outside of the sieve cylinder, and then start the electric driving rod. The electric driving rod drives the adjusting tooth rod to reciprocate inside the guiding track, and drives the rotating gear through the adjusting tooth rod to drive the hollow rotating tube to rotate the sieve cylinder bidirectionally, so that the cleaning brush plate cleans the outside of the sieve cylinder.

[0017] Step 3: At the same time, start the driving motor. The driving motor drives the rotating shaft to make the semi-circular gear rotate. When the tooth block end of the semi-circular gear meshes with the linkage tooth rod, at this time, the linkage tooth rod drives the push-pull column to slide inside the hollow rotating tube, and at the same time, the return spring is compressed. When the semi-circular gear separates from the linkage tooth rod, at this time, the return spring rebounds rapidly, so that the push-pull column drives the sieve cylinder to shake horizontally, thereby increasing the cleaning angle of the cleaning brush plate on the outside of the sieve cylinder.

[0018] As can be seen from the above, a separation device for stem cells provided by the present invention has the beneficial effects of realizing the cleaning of the filter plate while increasing the cleaning effect, avoiding the blockage of the filter plate caused by long-term filtration, and thus improving the filtration. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 FIG. is a schematic diagram of the main structure of a separation device for stem cells proposed by the present invention;

[0020] Figure 2 FIG. is a schematic diagram of the internal structure of the separation chamber of a separation device for stem cells proposed by the present invention;

[0021] Figure 3 FIG. is a schematic diagram of the structure of the filtration module of a separation device for stem cells proposed by the present invention;

[0022] Figure 4 FIG. is a schematic diagram of a partial structure of the filtration module of a separation device for stem cells proposed by the present invention;

[0023] Figure 5 FIG. is a schematic diagram of a partial structure of the filtration module of a separation device for stem cells proposed by the present invention;

[0024] Figure 6 is Figure 4 an enlarged schematic diagram of part A;

[0025] Figure 7 FIG. is a schematic diagram of the structure of the blanking module of a separation device for stem cells proposed by the present invention.

[0026] In the figure: 1. Separation bin; 2. Temperature control panel; 3. Tooling cover plate; 4. Observation pick-and-place panel; 5. Partition board; 6. Filtration module; 601. Sieving treatment frame; 602. Hollow rotating round tube; 603. Push-pull clamping column; 604. Sieving cylinder; 605. Feed pipe; 606. U-shaped limit seat; 607. Delivery pump; 608. Delivery pipe; 609. Electronic feeding pipe; 610. Tooling round long frame; 611. Guide track; 612. Adjusting rack; 613. Electric drive rod; 614. Support block; 615. Rotating gear; 616. Guide round rod; 617. Lifting support plate; 618. Cleaning brush plate; 619. Electric telescopic rod; 620. Adaptive top column; 621. Telescopic spring; 622. Linkage rack; 623. Slide block; 624. Return spring; 625. Fixed rod; 626. Rotating shaft; 627. Semi-circular gear; 628. Linkage gear; 629. Drive motor; 7. Discharging module; 701. Moving support plate; 702. Universal motor; 703. Rotating sliding seat; 704. Adjusting separation round seat; 705. Placing seat; 706. U-shaped mounting plate; 707. Extrusion spring; 708. Reagent tube body; 709. Arc-shaped rubber roller; 8. Limit track; 9. Horizontal moving seat; 10. Electric cylinder; 11. Heating panel. Detailed implementation manners

[0027] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments.

[0028] A separation device for stem cells disclosed by the present invention is mainly applied to the scenario where the filter plate is arranged inside the separation bin, it is not easy to clean the filter plate, and long-term filtration is likely to cause blockage of the filter plate, reducing the filtration effect.

[0029] Refer to Figures 1-6, A separation device for stem cells, including a separation chamber 1. On the opposite sides inside the separation chamber 1, there is a fixed connection of the same partition 5. And on one side of the partition 5, there is a filtering module 6. The filtering module 6 includes a sieving processing frame 601. One side of the sieving processing frame 601 is fixedly connected to one side of the partition 5. Both the sieving processing frame 601 and the two sides of the separation chamber 1 are provided with circular holes one. And inside the two circular holes one on the same side, there is a fixed connection of the same hollow rotating circular tube 602 through bearings. And on one side of the two hollow rotating circular tubes 602, there are notch openings. Inside the two notch openings, there is a sliding connection of a push-pull clamping column 603. On the opposite sides of the two push-pull clamping columns 603, there is a fixed connection of the same sieving cylinder 604. And on the outer part of the sieving cylinder 604, there are equidistantly arranged sieving holes. On the outer side of one side of the separation chamber 1, there is a fixed connection of a U-shaped limiting seat 606. And on one side of the U-shaped limiting seat 606, there is a circular opening. Inside the circular opening, there is a fixed connection of a feed pipe 605. On one side of one of the push-pull clamping columns 603, there is a feed port. The feed pipe 605 is movable inside the feed port.

[0030] Refer to Figures 1-6 , On one side of the sieving processing frame 601, there are two perforations. And inside the two perforations, there is a sliding connection of guiding round rods 616. At the ends of the two guiding round rods 616 located inside the sieving processing frame 601, there is a fixed connection of the same lifting support plate 617. On the lifting support plate 617, there are two smooth holes. Inside the two smooth holes, there is a sliding connection of adapting top columns 620. At one ends of the two adapting top columns 620, there is a fixed connection of the same cleaning brush plate 618. On the top of the lifting support plate 617 and on one side of the two adapting top columns 620, there is a fixed connection of the same telescopic spring 621. The telescopic spring 621 is wound around the outside of the adapting top column 620. On one side of the sieving processing frame 601, there is a fixed connection of an electric telescopic rod 619. The driving end of the electric telescopic rod 619 is fixedly connected to one side of the lifting support plate 617.

[0031] Refer to Figures 1-6 , On one side inside the separation chamber 1, there is a fixed connection of a guiding track 611. And inside the guiding track 611, there is a sliding connection of an adjusting toothed rod 612. On one side of the adjusting toothed rod 612, there is a fixed connection of a support block 614. On the outer part of one of the hollow rotating circular tubes 602, there is a fixed connection of a rotating gear 615. The rotating gear 615 meshes with the adjusting toothed rod 612. On one side inside the separation chamber 1, there is a fixed connection of an electric driving rod 613. The driving end of the electric driving rod 613 is fixedly connected to one side of the support block 614.

[0032] Refer to Figures 1-6, a discharge hole is formed on one side of the partition plate 5, and a conveying pipe 608 is fixedly connected to the inside of the discharge hole. One end of the conveying pipe 608 is fixedly connected to a conveying pump 607, and an electronic feeding pipe 609 is arranged at the discharging end of the conveying pipe 608. A tooling circular long frame 610 is fixedly connected to one side of one of the hollow rotating circular pipes 602, and two openings are formed in the outside of the tooling circular long frame 610.

[0033] Refer to Figures 1-6 , a linkage rack 622 is fixedly connected to one side of one of the push-pull columns 603, and a slider 623 is fixedly connected to one side of the linkage rack 622. The slider 623 slides inside the tooling circular long frame 610. A same return spring 624 is fixedly connected to the opposite sides of the slider 623 and the tooling circular long frame 610. Two fixing rods 625 are fixedly connected to the outside of the tooling circular long frame 610. Two round holes two are formed in one side of each of the two fixing rods 625. The same rotating shaft 626 is connected to the inside of the two opposite round holes two through bearings.

[0034] Refer to Figures 1-6 , linkage gears 628 are fixedly connected to one ends of the two rotating shafts 626, and the two linkage gears 628 are meshed with each other. Semi-circular gears 627 are fixedly connected to the outside of the two rotating shafts 626. The toothed block ends of the semi-circular gears 627 are meshed with the linkage rack 622. A driving motor 629 is arranged on one side of one of the fixing rods 625, and the driving end of the driving motor 629 is connected to one side of one of the rotating shafts 626 through a coupling.

[0035] In a specific application scenario, when filtering a cell suspension, first, the cell suspension is input into the inside of the sieving cylinder 604 through the feed pipe 605. Then, the cell suspension is sieved through the sieving cylinder 604, and the solids in the cell suspension are left inside the sieving cylinder 604. While filtering, the electric telescopic rod 619 is started. The electric telescopic rod 619 drives the cleaning brush plate 618 on the lifting support plate 617 to abut against the outside of the sieving cylinder 604. Then, the electric drive rod 613 is started. The electric drive rod 613 drives the adjusting tooth rod 612 to reciprocate inside the guiding track 611. The adjusting tooth rod 612 drives the rotating gear 615, so that the hollow rotating circular tube 602 drives the sieving cylinder 604 to rotate bidirectionally, thereby enabling the cleaning brush plate 618 to clean the outside of the sieving cylinder 604. At the same time, the drive motor 629 is started. The drive motor 629 drives the rotating shaft 626 to make the semi-circular gear 627 rotate. When the tooth block end of the semi-circular gear 627 meshes with the linkage tooth rod 622, at this time, the linkage tooth rod 622 drives the push-pull column 603 to slide inside the hollow rotating circular tube 602, and at the same time, the return spring 624 is compressed. When the semi-circular gear 627 is separated from the linkage tooth rod 622, at this time, the return spring 624 rebounds rapidly, so that the push-pull column 603 drives the sieving cylinder 604 to shake horizontally, thereby increasing the cleaning angle of the cleaning brush plate 618 on the outside of the sieving cylinder 604. Through the filtering module 6, the filter plate is cleaned and the cleaning effect is increased at the same time, avoiding the filter plate being easily blocked due to long-term filtering, thereby improving the filtering effect.

[0036] Refer to Figure 1 、 Figure 2 and Figure 7 As shown in FIGS.

[0037] Refer to Figure 1 、 Figure 2 and Figure 7 As shown in FIGS., on the adjusting separation circular seat 704, placing seats 705 are movably connected at equal intervals. And reagent tube bodies 708 are placed inside multiple placing seats 705. Inside multiple placing seats 705, compression springs 707 are distributed annularly and equidistantly. On one side of multiple compression springs 707 on the same side, a same U-shaped mounting plate 706 is fixedly connected. Inside multiple U-shaped mounting plates 706, arc-shaped rubber rollers 709 are movably connected at equal intervals. At the bottom of the moving support plate 701, a general motor 702 is provided. The driving end of the general motor 702 is fixedly connected to one side of the adjusting separation circular seat 704. An electric cylinder 10 is provided on one side of the separation chamber 1.

[0038] In a specific application scenario, when the filtered cell suspension is fed, the electric cylinder 10 is started at this time, and the reagent tube body 708 is moved below the electronic feeding tube 609, and then the filtered cell suspension is fed. The universal motor 702 is started, and the universal motor 702 drives the adjusting and separating circular seat 704 to rotate, and the reagent tube body 708 is fed in sequence, avoiding contact by the staff and reducing contamination. At the same time, the telescopic property of the compression spring 707 enables the arc-shaped rubber roller 709 to clamp and fix the reagent tube body 708 with different diameters.

[0039] Refer to Figure 1 、 Figure 2 and Figure 7 As shown in, one side of the separation chamber 1 is connected with a tooling cover plate 3 by bolts, and a heating panel 11 is arranged on the inner side of the tooling cover plate 3 facing the inside of the separation chamber 1. A temperature control panel 2 is arranged on the outer side of the separation chamber 1. An observation port is opened on one side of the separation chamber 1, and an observation access panel 4 is connected to the side of the observation port by a hinge.

[0040] A usage method of a separation device for stem cells, using a separation device for stem cells as described above, includes the following steps:

[0041] Step 1: When filtering the cell suspension, first input the cell suspension into the sieve tube 604 through the feed pipe 605, and then sieve the cell suspension through the sieve tube 604, leaving the solids in the cell suspension inside the sieve tube 604.

[0042] Step 2: While filtering, start the electric telescopic rod 619. The electric telescopic rod 619 drives the cleaning brush plate 618 on the lifting support plate 617 to abut against the outside of the sieve tube 604. Then start the electric drive rod 613. The electric drive rod 613 drives the adjusting tooth rod 612 to reciprocate inside the guiding track 611. The adjusting tooth rod 612 drives the rotating gear 615 to make the hollow rotating circular tube 602 drive the sieve tube 604 to rotate bidirectionally, so that the cleaning brush plate 618 cleans the outside of the sieve tube 604.

[0043] Step 3: At the same time, start the drive motor 629. The drive motor 629 drives the rotating shaft 626 to make the semi-circular gear 627 rotate. When the tooth block end of the semi-circular gear 627 meshes with the linkage tooth rod 622, at this time, the linkage tooth rod 622 drives the push-pull column 603 to slide inside the hollow rotating circular tube 602, and at the same time, the return spring 624 is compressed. When the semi-circular gear 627 is separated from the linkage tooth rod 622, at this time, the return spring 624 rebounds rapidly, so that the push-pull column 603 drives the sieve tube 604 to shake horizontally, thereby increasing the cleaning angle of the cleaning brush plate 618 on the outside of the sieve tube 604.

[0044] The above are only the preferred specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, making equivalent replacements or changes, shall be covered by the protection scope of the present invention.

Claims

1. A separation device for stem cells, comprising a separation chamber (1), characterized in that, Inside the separation chamber (1), the same partition plate (5) is fixedly connected to the opposite sides, and a filtering module (6) is arranged on one side of the partition plate (5). The filtering module (6) includes a sieving treatment frame (601). One side of the sieving treatment frame (601) is fixedly connected to one side of the partition plate (5). Circular holes one are formed in both the sieving treatment frame (601) and the two sides of the separation chamber (1). Inside the two circular holes one on the same side, the same hollow rotating circular tube (602) is connected through bearings. Grooves are formed on one side of the two hollow rotating circular tubes (602). Push-pull clamping columns (603) are slidably connected inside the two grooves. The opposite sides of the two push-pull clamping columns (603) are fixedly connected to the same sieving cylinder (604). Sieving holes are equidistantly formed on the outer part of the sieving cylinder (604). On the outer side of one side of the separation chamber (1), a U-shaped limiting seat (606) is fixedly connected. A circular opening is formed on one side of the U-shaped limiting seat (606). A feed pipe (605) is fixedly connected inside the circular opening. A feed port is formed on one side of one of the push-pull clamping columns (603). The feed pipe (605) is movably arranged inside the feed port.

2. The separation device for stem cells according to claim 1, characterized in that, Two through holes are formed on one side of the sieving treatment frame (601). Guide circular rods (616) are slidably connected inside the two through holes. The ends of the two guide circular rods (616) located inside the sieving treatment frame (601) are fixedly connected to the same lifting support plate (617). Two smooth holes are formed on the lifting support plate (617). Adaptation top columns (620) are slidably connected inside the two smooth holes. The ends of the two adaptation top columns (620) are fixedly connected to the same cleaning brush plate (618). One end of the lifting support plate (617) and one side of the two adaptation top columns (620) are fixedly connected to the same telescopic spring (621). The telescopic spring (621) is wound around the adaptation top column (620). An electric telescopic rod (619) is fixedly connected to one side of the sieving treatment frame (601). The driving end of the electric telescopic rod (619) is fixedly connected to one side of the lifting support plate (617).

3. The separation device for stem cells according to claim 2, wherein A guide track (611) is fixedly connected to one side inside the separation chamber (1). An adjusting toothed rod (612) is slidably connected inside the guide track (611). A support block (614) is fixedly connected to one side of the adjusting toothed rod (612). A rotating gear (615) is fixedly connected to the outer part of one of the hollow rotating circular tubes (602). The rotating gear (615) is meshed with the adjusting toothed rod (612). An electric driving rod (613) is fixedly connected to one side inside the separation chamber (1). The driving end of the electric driving rod (613) is fixedly connected to one side of the support block (614).

4. The separation device for stem cells according to claim 3, characterized in that, One side of the partition plate (5) is provided with a discharge hole, and a conveying pipe (608) is fixedly connected inside the discharge hole. One end of the conveying pipe (608) is fixedly connected with a conveying pump (607). An electronic feeding pipe (609) is arranged at the discharging end of the conveying pipe (608). One side of one of the hollow rotating circular pipes (602) is fixedly connected with a tooling circular long frame (610), and two openings are arranged on the outside of the tooling circular long frame (610).

5. The separation device for stem cells according to claim 4, characterized in that, One side of one of the push-pull clamping columns (603) is fixedly connected with a linkage rack (622). One side of the linkage rack (622) is fixedly connected with a slider (623). The slider (623) slides inside the tooling circular long frame (610). The opposite sides of the slider (623) and the tooling circular long frame (610) are fixedly connected with the same return spring (624). Two fixing rods (625) are fixedly connected to the outside of the tooling circular long frame (610). Two circular holes II are arranged on one side of each of the two fixing rods (625). The same rotating shaft (626) is connected through bearings inside the opposite two circular holes II.

6. The separation device for stem cells according to claim 5, characterized in that, One end of each of the two rotating shafts (626) is fixedly connected with a linkage gear (628), and the two linkage gears (628) are meshed with each other. Semicircular gears (627) are fixedly connected to the outside of the two rotating shafts (626). The tooth block end of the semicircular gear (627) is meshed with the linkage rack (622). A driving motor (629) is arranged on one side of one of the fixing rods (625). The driving end of the driving motor (629) is connected to one side of one of the rotating shafts (626) through a coupling.

7. An isolation device for stem cells according to claim 6, wherein, Two limiting tracks (8) are fixedly connected to one side inside the separation chamber (1). A horizontal moving seat (9) is slidably connected inside each of the two limiting tracks (8). The same blanking module (7) is arranged on the two horizontal moving seats (9). The blanking module (7) includes a moving support plate (701). A circular groove is arranged on one side of the moving support plate (701). A rotating sliding seat (703) is slidably connected inside the circular groove. An adjusting separation circular seat (704) is arranged above the rotating sliding seat (703).

8. The separation device for stem cells according to claim 7, characterized in that, Placement seats (705) are movably connected at equal intervals on the adjusting separation circular seat (704). Reagent tube bodies (708) are placed inside multiple placement seats (705). Compression springs (707) are annularly and equidistantly distributed inside multiple placement seats (705). One side of the compression springs (707) on the same side is fixedly connected with the same U-shaped mounting plate (706). Arc-shaped rubber rollers (709) are movably connected at equal intervals inside multiple U-shaped mounting plates (706). A universal motor (702) is arranged at the bottom of the moving support plate (701). The driving end of the universal motor (702) is fixedly connected to one side of the adjusting separation circular seat (704). An electric cylinder (10) is arranged on one side of the separation chamber (1).

9. The separation device for stem cells according to claim 8, wherein, One side of the separation chamber (1) is connected with a tooling cover plate (3) by bolts, and a heating panel (11) is arranged on the inner side of the tooling cover plate (3) facing the inside of the separation chamber (1). A temperature control panel (2) is arranged on the outer side of the separation chamber (1). An observation port is formed on one side of the separation chamber (1), and an observation access panel (4) is connected to the observation port by a hinge.

10. A method for using a separation device for stem cells, which uses a separation device for stem cells as described in claim 9, characterized in that, It includes the following steps: Step 1: When filtering the cell suspension, first input the cell suspension into the sieve tube (604) through the feed pipe (605), and then sieve the cell suspension through the sieve tube (604) to leave the solids in the cell suspension inside the sieve tube (604). Step 2: Start the electric telescopic rod (619) while filtering. The electric telescopic rod (619) drives the cleaning brush plate (618) on the lifting support plate (617) to abut against the outside of the sieve tube (604). Then start the electric drive rod (613). The electric drive rod (613) drives the adjusting tooth rod (612) to reciprocate inside the guiding track (611). The adjusting tooth rod (612) drives the rotating gear (615) to make the hollow rotating tube (602) drive the sieve tube (604) to rotate bidirectionally, so that the cleaning brush plate (618) cleans the outside of the sieve tube (604). Step 3: Start the drive motor (629) at the same time. The drive motor (629) drives the rotating shaft (626) to make the semi-circular gear (627) rotate. When the tooth block end of the semi-circular gear (627) meshes with the linkage tooth rod (622), at this time the linkage tooth rod (622) drives the push-pull column (603) to slide inside the hollow rotating tube (602), and at the same time the return spring (624) is compressed. When the semi-circular gear (627) separates from the linkage tooth rod (622), at this time the return spring (624) rebounds rapidly, so that the push-pull column (603) drives the sieve tube (604) to shake horizontally, thereby increasing the cleaning angle of the cleaning brush plate (618) on the outside of the sieve tube (604).

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