Preparation method and application of multi-effect antrodia camphorata composite drops

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a preparation method and application of multi-effect antrodia camphorata composite drops. The preparation method comprises the following steps: (1) preparing the antrodia camphorata extract; (2) preparing a mixed extract of houseleek and schisandra chinensis; (3) preparing a dandelion, radix puerariae and poria cocos mixed extract; (4) mixing the antrodia camphorata extract, the houseleek and schisandra chinensis mixed extract and the dandelion, kudzu vine root and poria cocos mixed extract to obtain mixed powder; and (5) heating polyethylene glycol until the polyethylene glycol is completely molten into liquid, adding the mixed powder, uniformly mixing, injecting into a spherical mold, cooling to form pills, demolding, and drying to obtain the multi-effect antrodia camphorata composite drop granules. The prepared antrodia camphorata extract is high in content of total triterpenes, and the multi-effect antrodia camphorata compound drops have a quick sobering effect and a liver protection effect at the same time.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a preparation method and application of a multi-effect Antrodia cinnamomea compound drip granule. Background Art

[0002] Antrodia cinnamomea (scientific name: Antrodia cinnamomea), also known as Antrodia camphorata, red camphor mushroom or fragrant cedar mushroom, etc., grows on the Cinnamomum kanehirai tree. This fungus has received extensive attention due to its remarkable medicinal value and is regarded as one of the rare medicinal materials, especially in Asian regions. Antrodia cinnamomea contains various active ingredients such as triterpenoids, polysaccharides, superoxide dismutase, etc., and these ingredients endow it with various biological activities such as antioxidant, anti-inflammatory, immunomodulatory and liver protection. Traditionally, Antrodia cinnamomea has been used to treat various diseases, including liver diseases, adjuvant treatment of cancer, improvement of blood circulation, etc.

[0003] Antrodia cinnamomea is considered to have a certain hangover effect, but there are some problems in the actual application of the current hangover drugs containing Antrodia cinnamomea, resulting in an unsatisfactory hangover effect. The existing Antrodia cinnamomea products generally have problems such as slow hangover speed and insignificant drug efficacy, and it is difficult to meet the actual needs. In view of the harm caused by the harmful substances generated during the alcohol metabolism process to the liver, it is very necessary to synchronously protect the liver during the hangover process, which not only helps to relieve the symptoms of acute alcohol poisoning, but also can prevent or reduce the chronic liver damage that may be caused by long-term drinking. However, the liver protection effect of the current Antrodia cinnamomea products is also not ideal. Summary of the Invention

[0004] The purpose of the present invention is to provide a preparation method and application of a multi-effect Antrodia cinnamomea compound drip granule.

[0005] In order to achieve the above purpose, the present invention provides the following technical solutions:

[0006] A preparation method of a multi-effect Antrodia cinnamomea compound drip granule, comprising the following steps:

[0007] (1) Preparation of Antrodia cinnamomea extract: Inoculate the seed liquid of Antrodia cinnamomea strain into the fermentation medium, shake and culture to obtain the fermentation broth, filter, collect the mycelium and fermentation broth respectively, dry the mycelium, mix the dried mycelium with ethanol, heat to reflux extraction, concentrate and dry to obtain the Antrodia cinnamomea extract;

[0008] (2) Preparation of the mixed extract of Echeveria glauca and Schisandra chinensis: Mix Echeveria glauca and Schisandra chinensis in a weight ratio of 1:(2 - 5), crush them, add water, add Lactobacillus plantarum, ferment, sterilize, filter and dry to obtain the mixed extract of Echeveria glauca and Schisandra chinensis;

[0009] (3) Preparation of the mixed extract of dandelion, kudzu root and poria cocos: Mix dandelion, kudzu root and poria cocos in a weight ratio of (3 - 4):1:(1.5 - 2), pulverize them, add water, add neutral protease, carry out enzymatic hydrolysis, inactivate the enzyme, filter, and dry to obtain the mixed extract of dandelion, kudzu root and poria cocos;

[0010] (4) Mix the antrodia cinnamomea extract, the mixed extract of echeveria and schisandra chinensis, and the mixed extract of dandelion, kudzu root and poria cocos to obtain a mixed powder;

[0011] (5) Heat polyethylene glycol until it is completely melted into a liquid, add the mixed powder, mix well, inject it into a spherical mold, cool it into pills, demold, and dry to obtain the multi - effect antrodia cinnamomea compound drip particles.

[0012] Preferably, mix 10 parts by weight of the antrodia cinnamomea extract, 1.5 - 4.5 parts by weight of the mixed extract of echeveria and schisandra chinensis, and 4 - 7 parts by weight of the mixed extract of dandelion, kudzu root and poria cocos to obtain a mixed powder.

[0013] Preferably, the fermentation medium includes: using deionized water as the medium, 10 - 15 g / L of glucose, 15 - 20 g / L of sucrose, 2 - 6 g / L of beef extract, 0.8 - 1.2 g / L of fatty acid, 12 - 17 g / L of gluten powder, 8 - 13 g / L of corn flour, 0.5 - 1.5 g / L of potassium dihydrogen phosphate, 0.5 - 1.5 g / L of magnesium sulfate heptahydrate, 1 - 2 g / L of calcium sulfate, and sterilize at 121 °C for 20 min.

[0014] Preferably, the fatty acid includes oleic acid, linoleic acid and stearic acid in a weight ratio of 1:(1.2 - 1.5):(0.3 - 0.5).

[0015] Preferably, based on the added amount of water, the dosage of lactobacillus plantarum is 10 7 - 10 8 CFU / mL of water.

[0016] Preferably, the fermentation conditions are: ferment at 30 - 35 °C for 15 - 20 h.

[0017] Preferably, based on the added amount of water, the dosage of neutral protease is 150 - 200 U / mL of water.

[0018] Preferably, the enzymatic hydrolysis conditions are enzymatic hydrolysis at 40 - 45 °C for 3 - 5 h.

[0019] Preferably, the dosage of polyethylene glycol is 1.5 - 2 times the weight of the mixed powder.

[0020] The present invention provides the multi - effect antrodia cinnamomea compound drip particles prepared according to the above - mentioned preparation method.

[0021] The present invention provides the application of the described multi-effect Antrodia cinnamomea compound drops in the preparation of an anti-hangover and liver-protecting drug.

[0022] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows:

[0023] 1. By adding a specific content of fatty acid to the fermentation medium of the Antrodia cinnamomea strain, the present invention can increase the content of total triterpenoids in the Antrodia cinnamomea extract and improve the anti-hangover speed and liver-protecting effect of the multi-effect Antrodia cinnamomea compound drops.

[0024] 2. By separately preparing the mixed extract of Echeveria glauca and Schisandra chinensis, and the mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos, and mixing them with the Antrodia cinnamomea extract, the active components in the extract can assist the total triterpenoids in the Antrodia cinnamomea extract to produce a synergistic effect, improving the anti-hangover speed and liver-protecting effect of the multi-effect Antrodia cinnamomea compound drops. Specific Embodiments

[0025] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0026] All raw materials used in the following embodiments of the present invention are commercially available products:

[0027] Antrodia cinnamomea strain, product number ATCC200183, Beijing BioWin Biotechnology Co., Ltd.

[0028] The preparation method of the Antrodia cinnamomea strain seed liquid includes the following steps: inoculate the Antrodia cinnamomea strain 1 cm 2 (colony area) into the PDA medium under sterile conditions, culture at 28 °C for 7 days to obtain an activated strain, mix 10 mL of the activated strain and 90 mL of the seed medium, and culture in a shaker at 120 r / min and 28 °C for 5 days.

[0029] PDA slant medium, Shanghai Yuanye Bio-Technology Co., Ltd., product number R30024.

[0030] The seed medium includes: using water as the medium, 28 g / L of glucose, 3 g / L of peptone, 1.2 g / L of potassium dihydrogen phosphate, 1.3 g / L of magnesium sulfate heptahydrate, and sterilize at 121 °C for 20 min.

[0031] Lactobacillus plantarum, product number TS278112, Ningbo Taisituo Biotechnology Co., Ltd.

[0032] Lactobacillus delbrueckii, product number TS287480, Ningbo Taisituo Biotechnology Co., Ltd.

[0033] Neutral protease, product number S10013, Shanghai Yuanye Bio-Technology Co., Ltd.

[0034] Example 1

[0035] This example provides a preparation method of a multi-effect Antrodia camphorata compound drip granule, including the following steps:

[0036] (1) Preparation of Antrodia camphorata extract: Inoculate the Antrodia camphorata strain seed liquid into the fermentation medium, and the volume ratio of the Antrodia camphorata strain seed liquid to the fermentation medium is 1:9. Incubate in a shaker at 120 r / min and 28 °C for 7 days to obtain the fermentation broth. Filter the fermentation broth through four layers of gauze, collect the mycelium and the fermentation broth respectively, dry the mycelium, mix the mycelium with ethanol at a weight ratio of 1:9, heat to reflux extraction for 80 min, concentrate and dry to obtain the Antrodia camphorata extract;

[0037] The fermentation medium: Using deionized water as the medium, glucose 12 g / L, sucrose 18 g / L, beef extract 3 g / L, fatty acids 1 g / L, gluten 15 g / L, corn flour 10 g / L, potassium dihydrogen phosphate 1 g / L, magnesium sulfate heptahydrate 1 g / L, calcium sulfate 1.6 g / L, sterilize at 121 °C for 20 min. The fatty acids include oleic acid, linoleic acid and stearic acid with a weight ratio of 1:1.4:0.4.

[0038] (2) Preparation of the mixed extract of Echeveria peacockii and Schisandra chinensis: Mix Echeveria peacockii and Schisandra chinensis with a weight ratio of 1:3, crush them to less than 100 mesh, add water 10 times the total weight of Echeveria peacockii and Schisandra chinensis, add Lactobacillus plantarum, and based on the water addition amount, the dosage of Lactobacillus plantarum is 10 8 CFU / mL water, ferment at 32 °C for 18 h, sterilize, filter, and dry to obtain the mixed extract of Echeveria peacockii and Schisandra chinensis;

[0039] (3) Preparation of the mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos: Mix Taraxacum mongolicum, Pueraria lobata and Poria cocos with a weight ratio of 3.5:1:1.9, crush them to less than 100 mesh, add water 10 times the total weight of Taraxacum mongolicum, Pueraria lobata and Poria cocos, add neutral protease, and based on the water addition amount, the dosage of neutral protease is 180 U / mL water, enzymolyze at 42 °C for 4 h, inactivate the enzyme, filter, and dry to obtain the mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos;

[0040] (4) Mix 10 parts by weight of the Antrodia camphorata extract, 3 parts by weight of the mixed extract of Echeveria peacockii and Schisandra chinensis, and 5 parts by weight of the mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos to obtain a mixed powder;

[0041] (5) Take polyethylene glycol 6000 which is 1.5 times the weight of the mixed powder, heat it until it completely melts into a liquid, add the mixed powder, mix well, inject it into a spherical mold with a metering pump under the condition of 80 °C, cool it to form pills at 2 °C, demold, and dry it at 20 °C for 100 min to obtain multi-effect Antrodia camphorata composite pellets with a single pellet weight of 60 mg.

[0042] Example 2

[0043] This example provides a preparation method of multi-effect Antrodia camphorata composite pellets, including the following steps:

[0044] (1) Preparation of Antrodia camphorata extract: Inoculate the Antrodia camphorata strain seed liquid into the fermentation medium, the volume ratio of the Antrodia camphorata strain seed liquid to the fermentation medium is 1:9, shake and culture in a shaker at 120 r / min and 28 °C for 7 days to obtain the fermentation broth. Filter the fermentation broth with four layers of gauze, collect the mycelium and the fermentation broth respectively, dry the mycelium, mix the mycelium and ethanol according to the weight ratio of 1:10, heat it to reflux extraction for 90 min, concentrate and dry it to obtain the Antrodia camphorata extract;

[0045] The fermentation medium: Using deionized water as the medium, glucose 10 g / L, sucrose 20 g / L, beef extract 2 g / L, fatty acids 1.2 g / L, gluten powder 12 g / L, corn flour 13 g / L, potassium dihydrogen phosphate 0.5 g / L, magnesium sulfate heptahydrate 1.5 g / L, calcium sulfate 1 g / L, sterilize at 121 °C for 20 min. The fatty acids include oleic acid, linoleic acid and stearic acid with a weight ratio of 1:1.2:0.5.

[0046] (2) Preparation of the mixed extract of Echeveria secunda and Schisandra chinensis: Mix Echeveria secunda and Schisandra chinensis with a weight ratio of 1:2 and crush them to less than 100 mesh, add water which is 10 times the total weight of Echeveria secunda and Schisandra chinensis, add Lactobacillus plantarum, and the dosage of Lactobacillus plantarum is 10 7 CFU / mL of water, ferment at 35 °C for 15 h, sterilize, filter, and dry to obtain the mixed extract of Echeveria secunda and Schisandra chinensis;

[0047] (3) Preparation of the mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos: Mix Taraxacum mongolicum, Pueraria lobata and Poria cocos with a weight ratio of 3:1:2 and crush them to less than 100 mesh, add water which is 10 times the total weight of Taraxacum mongolicum, Pueraria lobata and Poria cocos, add neutral protease, and the dosage of neutral protease is 200 U / mL of water based on the added amount of water, enzymatically hydrolyze at 40 °C for 5 h, inactivate the enzyme, filter, and dry to obtain the mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos;

[0048] (4) Mix 10 parts by weight of Antrodia camphorata extract, 1.5 parts by weight of the mixed extract of Echeveria secunda and Schisandra chinensis, and 7 parts by weight of the mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos to obtain the mixed powder;

[0049] (5) Heat 1.5 times the weight of the mixed powder of polyethylene glycol 6000 until it is completely melted into a liquid. Add the mixed powder and mix well. Inject it into a spherical mold with a metering pump under the condition of 80 °C, cool it to form pellets at 2 °C, demold, and dry it at 20 °C for 100 min to obtain multi-effect Antrodia camphorata composite pellets with a single pellet weight of 60 mg.

[0050] Comparative Example 1

[0051] The difference between this comparative example and Example 1 is that the fatty acid is replaced by linolenic acid.

[0052] Comparative Example 2

[0053] The difference between this comparative example and Example 1 is that the fatty acid includes oleic acid, linoleic acid, and stearic acid with a weight ratio of 1.2:0.5:1.

[0054] Comparative Example 3

[0055] The difference between this comparative example and Example 1 is that the fatty acid includes linoleic acid, linolenic acid, and oleic acid with a weight ratio of 1:1.2:0.5.

[0056] Comparative Example 4

[0057] The difference between this comparative example and Example 1 is that Lactobacillus plantarum is replaced by Lactobacillus delbrueckii.

[0058] Comparative Example 5

[0059] The difference between this comparative example and Example 1 is a preparation method of multi-effect Antrodia camphorata composite pellets, including the following steps:

[0060] (1) Preparation of Antrodia camphorata extract: Inoculate the Antrodia camphorata strain seed liquid into the fermentation medium. The volume ratio of the Antrodia camphorata strain seed liquid to the fermentation medium is 1:9. Oscillate and culture it in a shaker at 120 r / min and 28 °C for 7 days to obtain the fermentation broth. Filter the fermentation broth with four layers of gauze, collect the mycelium and the fermentation broth separately, dry the mycelium, mix the mycelium and ethanol according to a weight ratio of 1:10, heat it to reflux and extract for 90 min, concentrate and dry it to obtain the Antrodia camphorata extract;

[0061] The fermentation medium: Using deionized water as the medium, glucose 10 g / L, sucrose 20 g / L, beef extract 2 g / L, fatty acid 1.2 g / L, gluten powder 12 g / L, corn flour 13 g / L, potassium dihydrogen phosphate 0.5 g / L, magnesium sulfate heptahydrate 1.5 g / L, calcium sulfate 1 g / L, sterilize at 121 °C for 20 min. The fatty acid includes oleic acid, linoleic acid, and stearic acid with a weight ratio of 1:1.2:0.5.

[0062] (2) Mix Echeveria and Schisandra chinensis in a weight ratio of 1:2 and crush them to less than 100 mesh to obtain Powder A; mix Taraxacum mongolicum, Pueraria lobata, and Poria cocos in a weight ratio of 3:1:2 and crush them to less than 100 mesh to obtain Powder B. Mix 1.5 parts by weight of Powder A and 7 parts by weight of Powder B, add water 10 times the total weight of all powders, add Lactobacillus plantarum. Based on the water addition amount, the dosage of Lactobacillus plantarum is 10 7 CFU / mL of water, ferment at 35 °C for 15 h, sterilize, filter, and dry to obtain a mixed extract;

[0063] (3) Mix 10 parts by weight of Antrodia camphorata extract and 8.5 parts by weight of the mixed extract to obtain a mixed powder;

[0064] (4) Heat polyethylene glycol 6000, which is 1.5 times the weight of the mixed powder, until it is completely melted into a liquid. Add the mixed powder, mix well, and inject it into a spherical mold with a metering pump at 80 °C. Cool it to form pills at 2 °C, demold, and dry at 20 °C for 100 min to obtain multi-effect Antrodia camphorata compound pellets with a single pellet weight of 60 mg.

[0065] Comparative Example 6

[0066] The difference between this comparative example and Example 1 is: A preparation method of multi-effect Antrodia camphorata compound pellets, including the following steps:

[0067] (1) Preparation of Antrodia camphorata extract: Inoculate the Antrodia camphorata strain seed liquid into the fermentation medium. The volume ratio of the Antrodia camphorata strain seed liquid to the fermentation medium is 1:9. Shake and culture in a shaker at 120 r / min and 28 °C for 7 days to obtain the fermentation broth. Filter the fermentation broth with 4 layers of gauze, collect the mycelium and the fermentation broth separately. Dry the mycelium, mix the mycelium and ethanol in a weight ratio of 1:10, heat to reflux extraction for 90 min, concentrate and dry to obtain the Antrodia camphorata extract;

[0068] The fermentation medium: Using deionized water as the medium, glucose 10 g / L, sucrose 20 g / L, beef extract 2 g / L, fatty acids 1.2 g / L, gluten powder 12 g / L, corn flour 13 g / L, potassium dihydrogen phosphate 0.5 g / L, magnesium sulfate heptahydrate 1.5 g / L, calcium sulfate 1 g / L, sterilize at 121 °C for 20 min. The fatty acids include oleic acid, linoleic acid, and stearic acid in a weight ratio of 1:1.2:0.5.

[0069] (2) Mix Echeveria secunda and Schisandra chinensis in a weight ratio of 1:2 and crush them to less than 100 mesh to obtain powder A; mix Taraxacum mongolicum, Pueraria lobata, and Poria cocos in a weight ratio of 3:1:2 and crush them to less than 100 mesh to obtain powder B. Mix 1.5 parts by weight of powder A and 7 parts by weight of powder B, add 10 times the total weight of all powders of water, add neutral protease, and based on the amount of water added, the dosage of neutral protease is 200 U / mL of water. Enzymatically hydrolyze at 40 °C for 5 h, inactivate the enzyme, filter, and dry to obtain a mixed extract;

[0070] (3) Mix 10 parts by weight of Antrodia camphorata extract and 8.5 parts by weight of the mixed extract to obtain a mixed powder;

[0071] (4) Take 1.5 times the weight of the mixed powder of polyethylene glycol 6000 and heat it until it completely melts into a liquid. Add the mixed powder, mix well, inject it into a spherical mold with a metering pump at 80 °C, cool it to form pills at 2 °C, demold, and dry it at 20 °C for 100 min to obtain multi-effect Antrodia camphorata compound pellets with a single pellet weight of 60 mg.

[0072] Comparative Example 7

[0073] The difference between this comparative example and Example 1 is: Mix 10 parts by weight of Antrodia camphorata extract, 6 parts by weight of the mixed extract of Echeveria secunda and Schisandra chinensis, and 2 parts by weight of the mixed extract of Taraxacum mongolicum, Pueraria lobata, and Poria cocos to obtain a mixed powder.

[0074] Performance Test

[0075] The determination of the total triterpenoid yield is carried out by the vanillin - glacial acetic acid method to determine the total triterpenoid yield in the mycelium. Reference: The measurement method in the research on optimizing the extraction process of triterpenoids from Ganoderma lucidum fruiting bodies by Hong Wenlong et al. using the response surface method. Specifically, the standard curve is drawn using oleanolic acid as the standard product. Mix 0.25 g of the mycelium prepared in Examples 1 - 2 and Comparative Examples 1 - 3 with ethanol in a weight ratio of 1:9, heat to reflux for extraction for 80 min, concentrate and dry to obtain Antrodia camphorata extract. Add 0.15 mL of 5% vanillin glacial acetic acid solution to the Antrodia camphorata extract, mix well with 0.5 mL of perchloric acid, place it in a water bath at 70 °C and heat for 20 min, quickly put it into ice water to cool, add glacial acetic acid to make the volume up to 5 mL, quickly mix the solution evenly, measure the absorbance value at 550 nm, calculate the mass concentration of the total triterpenoids according to the standard curve, and the calculation formula for the total triterpenoid yield is: W = (c × V × f) / m × 100%, where c is the mass concentration of the total triterpenoids calculated according to the standard curve, μg / mL; V is the volume of the extraction solution, mL; f is the dilution factor; m is the mass of the mycelium, g.

[0076] Table 1 Test results of total triterpenoid yield

[0077]

[0078]

[0079] As can be seen from Table 1, the mycelia of Examples 1-2 were fermented using a fermentation broth containing linoleic acid, linolenic acid and oleic acid in specific proportions, and the total triterpenoid content in the Antrodia cinnamomea extract prepared by ethanol extraction was high. The high total triterpenoid content in the Antrodia cinnamomea extract means that triterpenoids can promote the decomposition and excretion of ethanol and its metabolite acetaldehyde in the body, helping to accelerate the body's alcohol clearance rate and thus speeding up the alcohol detoxification process. At the same time, triterpenoids have strong antioxidant ability, which can help reduce the oxidative stress response caused by alcohol intake, protect cells from free radical damage, indirectly support the liver to quickly return to normal function, and thus may improve the alcohol detoxification efficiency. Triterpenoids have significant anti-inflammatory properties and can regulate the immune system, reducing the inflammatory response caused by alcohol to the liver and helping to maintain liver health. Triterpenoid components can promote the regeneration of liver cells and help repair damaged liver tissue. This is particularly important for liver damage caused by long-term alcohol consumption, as continuous repair mechanisms help prevent the development of more serious liver diseases. At the same time, triterpenoids can also enhance the liver's detoxification function, enabling the liver to more effectively process and excrete toxins, including harmful substances such as acetaldehyde produced during alcohol metabolism. Therefore, the high total triterpenoid content in the Antrodia cinnamomea extract plays an important role in enhancing the alcohol detoxification speed and improving the liver protection effect.

[0080] In Comparative Example 1, linoleic acid was used for fermentation, and the total triterpenoid content in the Antrodia cinnamomea extract was lower than that in Example 1.

[0081] In Comparative Example 2, the fatty acids were replaced with oleic acid, linoleic acid and stearic acid in a weight ratio of 1.2:0.5:1, and the total triterpenoid content in the Antrodia cinnamomea extract was lower than that in Example 1.

[0082] In Comparative Example 3, the fatty acids included linoleic acid, linolenic acid and oleic acid in a weight ratio of 1:1.2:0.5, and the total triterpenoid content in the Antrodia cinnamomea extract was lower than that in Example 1.

[0083] 2. Detection of alcohol detoxification effect.

[0084] 100 rats that had not eaten for 6 hours were selected and randomly divided into 10 groups, with 5 males and 5 females in each group; 9 groups were respectively gavaged with the products prepared in Examples 1-2 and Comparative Examples 1-7 at a dose of 0.02 g / Kg body weight; 1 group was not gavaged as the blank group. 30 minutes after gavage, each rat was gavaged with 6 g / Kg of edible alcohol with a volume concentration of 55%; blood samples were taken from the rats at 0.5 h, 1 h, 1.5 h and 3 h after alcohol gavage; gas chromatography was used to measure the change in blood alcohol concentration; the detection results are shown in Table 2 below.

[0085] Table 2 Detection results of changes in blood alcohol concentration (g / L)

[0086] 0.5h 1h 1.5h 3h Example 1 0.25 0.31 0.17 0.01 Example 2 0.27 0.33 0.19 0.02 Comparative Example 1 0.45 0.58 0.35 0.21 Comparative Example 2 0.43 0.56 0.32 0.18 Comparative Example 3 0.40 0.54 0.30 0.15 Comparative Example 4 0.31 0.43 0.22 0.07 Comparative Example 5 0.36 0.48 0.25 0.11 Comparative Example 6 0.34 0.47 0.24 0.08 Comparative Example 7 0.38 0.50 0.29 0.12 Blank group 0.89 1.02 0.83 0.57

[0087] As can be seen from Table 2, the hangover-relieving effect of Examples 1-2 is faster.

[0088] In Comparative Examples 1-3, the hangover-relieving effect is consistent with the results of the total triterpenoid content in Table 1 compared with Example 1.

[0089] In Comparative Example 4, the strain was changed, and the hangover-relieving effect was worse than that of Example 1, indicating that only by using specific strains for fermentation can the prepared active components better assist the antler mushroom extract to exert the hangover-relieving effect.

[0090] In Comparative Example 5, only mixed fermentation was carried out, and in Comparative Example 6, only mixed enzymatic hydrolysis was carried out. The hangover-relieving effect was worse than that of Example 1, indicating that when the raw materials are grouped for different extraction methods, there are more active components in the prepared extract that contribute to hangover relief.

[0091] In Comparative Example 7, the amounts of the mixed extract of echeveria and schisandra chinensis, dandelion, pueraria lobata and poria cocos were different, and the hangover-relieving effect of the multi-effect antler mushroom compound drip pills became worse.

[0092] The above is the preferred embodiment of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle described in the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A preparation method of a multi-effect Antrodia cinnamomea compound drip granule, characterized in that, It includes the following steps: (1) Preparation of Antrodia cinnamomea extract: Inoculate the Antrodia cinnamomea strain seed liquid into the fermentation medium, shake culture to obtain the fermentation broth, filter, collect the mycelium and the fermentation broth respectively, dry the mycelium, mix the dried mycelium with ethanol, heat and extract, concentrate and dry to obtain the Antrodia cinnamomea extract; (2) Preparation of mixed extract of Echeveria glauca and Schisandra chinensis: Mix Echeveria glauca and Schisandra chinensis in a weight ratio of 1:(2 - 5), crush them, add water, add Lactobacillus plantarum, ferment, sterilize, filter and dry to obtain the mixed extract of Echeveria glauca and Schisandra chinensis; (3) Preparation of mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos: Mix Taraxacum mongolicum, Pueraria lobata and Poria cocos in a weight ratio of (3 - 4):1:(1.5 - 2), crush them, add water, add neutral protease, enzymatically hydrolyze, inactivate the enzyme, filter and dry to obtain the mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos; (4) Mix the Antrodia cinnamomea extract, the mixed extract of Echeveria glauca and Schisandra chinensis, and the mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos to obtain a mixed powder; (5) Heat polyethylene glycol until it completely melts into a liquid, add the mixed powder, mix well, inject it into a spherical mold, cool it into pills, demold and dry to obtain the multi - effect Antrodia cinnamomea compound drops.

2. The preparation method of the multi-effect Antrodia cinnamomea compound drip granule according to claim 1, wherein, Mix 10 parts by weight of Antrodia cinnamomea extract, 1.5 - 4.5 parts by weight of the mixed extract of Echeveria glauca and Schisandra chinensis, and 4 - 7 parts by weight of the mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos to obtain a mixed powder.

3. The preparation method of the multi-effect Antrodia cinnamomea compound drip granule according to claim 1, characterized in that, The fermentation medium includes: using deionized water as the medium, 10 - 15 g / L of glucose, 15 - 20 g / L of sucrose, 2 - 6 g / L of beef extract, 0.8 - 1.2 g / L of fatty acid, 12 - 17 g / L of gluten powder, 8 - 13 g / L of corn flour, 0.5 - 1.5 g / L of potassium dihydrogen phosphate, 0.5 - 1.5 g / L of magnesium sulfate heptahydrate, and 1 - 2 g / L of calcium sulfate.

4. The preparation method of the multi-effect Antrodia cinnamomea compound drip granule according to claim 3, wherein The fatty acid includes oleic acid, linoleic acid and stearic acid in a weight ratio of 1:(1.2 - 1.5):(0.3 - 0.5).

5. The preparation method of the multi-effect Antrodia cinnamomea compound drip granule according to claim 1, characterized in that, The dosage of Lactobacillus plantarum is 10 7 -10 8 CFU / mL of water.

6. The preparation method of the multi-effect Antrodia cinnamomea compound drip granule according to claim 5, wherein, The fermentation conditions are: ferment at 30 - 35 °C for 15 - 20 h.

7. The preparation method of the multi-effect Antrodia cinnamomea compound drip granule according to claim 1, characterized in that, Calculated by the water addition amount, the dosage of neutral protease is 150 - 200 U / mL of water.

8. The preparation method of the multi-effect Antrodia cinnamomea compound drip granule according to claim 7, characterized in that, The conditions for enzymatic hydrolysis are: enzymatic hydrolysis at 40 - 45 °C for 3 - 5 h.

9. The preparation method of the multi-effect Antrodia camphorata compound drip granule according to claim 1, wherein, The dosage of polyethylene glycol is 1.5 - 2 times the weight of the mixed powder.

10. Use of the multi - effect Antrodia cinnamomea compound drops prepared by the preparation method according to any one of claims 1 - 9 in the preparation of anti - hangover and liver - protecting drugs.

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