Preparation device and preparation method of platelet-rich fibrin

Abstract

The invention discloses a device and a method for preparing platelet-rich fibrin, and belongs to the technical field of medical instruments. The invention solves the problem of bacterial contamination risk in the existing preparation process of platelet-rich fibrin. The injection pipe set, the piston pipe and the centrifugal pipe body are sequentially and coaxially arranged from inside to outside, the piston pipe is in sliding connection with the centrifugal pipe body, the bottom of the push rod and the injection pipe body, the bottom of the injection pipe body and the bottom of the piston pipe, and the outer wall of the bottom of the piston pipe and the inner wall of the centrifugal pipe body are all arranged in a sealed mode. An injection cavity is formed in the injection tube body below the push rod, and a through cavity is formed in the centrifugal tube body below the piston tube. The functions of venous blood whole blood collection, centrifugal separation, red blood cell excision and injection are integrated, the operation requirement that a plurality of functional devices or steps need to be used in a traditional method is reduced, the whole process from blood collection to PRF extraction is completed, and the risk that external pollutants enter can be effectively avoided through the sealed connection design.

Description

Technical Field

[0001] The present invention relates to a preparation device and a preparation method of platelet-rich fibrin, belonging to the technical field of medical devices. Background Art

[0002] Platelet-rich fibrin (PRF) is an autologous platelet concentrate that can promote wound healing and is widely used in the fields of intrauterine adhesions, chronic wound infections (regeneration of skin burns, treatment of gangrene in diabetic patients), periodontal tissue regeneration in dentistry, dental implants, bone tissue regeneration, gingival mucosa regeneration, and repair of hard and soft tissues in tooth extraction sockets.

[0003] PRF has three main components, including ① cellular components: PRF not only contains abundant platelets but also a small amount of white blood cells and red blood cells; ② temporary extracellular matrix: a three-dimensional scaffold composed of autologous fibrin in blood, including fibrin and vitronectin; ③ bioactive molecules: more than 100 kinds of bioactive protein factors. PRF is observed macroscopically as a light yellow gel and presents a three-dimensional stereoscopic network structure with fibrin as the skeleton under a scanning electron microscope. The equilateral connection method is particularly conducive to the migration and regeneration of the endometrium and can serve as a bioactive scaffold for the migration and crawling of newborn cells.

[0004] Currently, in clinical operations, the existing preparation and acquisition process of platelet-rich fibrin (PRF) is relatively complex. After blood collection, centrifugation is required immediately. After centrifugation, it is divided into two layers. The blood collection tube containing PRF is sent to the operating room, and the operator uses sterile forceps to take out the PRF from the tube at the bedside of the operating table, and uses sterile scissors to remove the part of the red blood cell clot at the base, and then uses a self-made injection tube of a 1 ml syringe to place the PRF into the uterine cavity. The entire operation process needs to ensure a sterile environment, but the risk of surgical infection still cannot be avoided. Therefore, there is an urgent need for a new type of preparation device and preparation method of platelet-rich fibrin to reduce the operation requirements of using multiple functional instruments or steps in the traditional method, so that the PRF preparation process is always in a sterile environment to improve the quality and stability of the final product. Summary of the Invention

[0005] The present invention is to solve the problem that there is a risk of infection in the existing preparation process of platelet-rich fibrin, and further provides a preparation device and a preparation method of platelet-rich fibrin.

[0006] The technical solution adopted by the present invention to solve the above technical problems is:

[0007] A preparation device for platelet-rich fibrin, comprising a centrifuge tube body, a piston tube, an injection tube group, a blood collection component and a rotary cutting component. Among them, the injection tube group, the piston tube and the centrifuge tube body are coaxially arranged in sequence from the inside to the outside. The piston tube is slidably connected to the centrifuge tube body. The injection tube group includes an injection tube body and a push rod that is coaxially and axially slidably inserted into the injection tube body. The bottom of the injection tube body is detachably connected to the piston tube. Sealing structures are provided between the outer wall of the bottom of the push rod and the inner wall of the injection tube body, between the bottom of the injection tube body and the bottom of the piston tube, and between the outer wall of the bottom of the piston tube and the inner wall of the centrifuge tube body. A circulation channel is axially opened inside the push rod. An injection cavity is formed inside the injection tube body below the push rod, and a through cavity is formed inside the centrifuge tube body below the piston tube. The circulation channel, the injection cavity and the through cavity are axially connected;

[0008] The blood collection component is detachably connected to the push rod. When the blood collection component is connected to the push rod, the blood collection component is in communication with the circulation channel;

[0009] The rotary cutting component is sleeved on the lower opening position of the centrifuge tube body in a relatively movable manner, and a sealing structure is provided between the rotary cutting component and the centrifuge tube body.

[0010] Furthermore, the rotary cutting component includes a rotary connection cap and a rotary cutting structure. The rotary connection cap is detachably connected to the bottom of the centrifuge tube body. The rotary cutting structure is inserted through the rotary connection cap, and the rotary cutting end of the rotary cutting structure is located in the through cavity.

[0011] Furthermore, the rotary cutting structure includes a control rod and a cutting tool installed at one end of the control rod. The cutting tool is located in the through cavity, and the control rod is threadedly connected to the rotary connection cap.

[0012] Furthermore, the rotary connection cap is threadedly connected to the bottom of the centrifuge tube body. A cap plug is provided on the rotary connection cap, and the cap plug protrudes into the through cavity. Sealing structures are provided between the inner wall of the cap plug and the inner wall of the centrifuge tube body and between the outer wall of the centrifuge tube body and the rotary connection cap.

[0013] Furthermore, sliders are provided on the outer wall of the piston tube, and corresponding chutes for cooperating with the sliders are provided on the inner wall of the centrifuge tube body.

[0014] Furthermore, the top of the push rod is blocked with a rubber soft plug.

[0015] Furthermore, a threaded joint is machined at the bottom of the injection tube body, and a first piston-type rubber plug is provided at the bottom of the piston tube. The first piston-type rubber plug is provided with a threaded groove that matches the shape of the threaded joint, and the threaded groove is adapted to the threaded joint.

[0016] Furthermore, the preparation device also includes a braking component, which includes a support rod hinged to the centrifuge tube body and a first locking nut and a second locking nut respectively screwed to the support rod. The upper opening of the centrifuge tube body is provided with a first pipe connector, and the bottom end of the support rod is rotatably connected to the first pipe connector. The top of the injection tube body is provided with a third pipe connector, and the third pipe connector is provided with an open groove for the support rod to pass through. The open groove is a U-shaped structure, and the groove edge smoothly transitions to the outer circumference of the third pipe connector, and is screwed to the support rod through the first locking nut and the second locking nut to clamp the third pipe connector.

[0017] Furthermore, the blood sampling component includes a blood sampling needle, a blood sampling tube and a connector which are connected end to end in sequence, wherein the connector is detachably connected to the push rod, and when the connector is connected to the push rod, the interior of the connector is connected to the flow channel.

[0018] A method for preparing platelet-rich fibrin comprises using the above-mentioned device to perform the following steps:

[0019] Step 1, device preparation: move the rotary cutting structure to the lower opening position adjacent to the centrifuge tube body, pull the injection tube body to form negative pressure, so that venous whole blood can enter the centrifuge tube body during blood collection;

[0020] Step 2, collecting blood: insert the blood collection member into the patient's vein, and after confirming the blood return, connect the blood collection member with the top of the push rod, so that the venous blood is introduced into the centrifuge tube body from the blood collection member;

[0021] Step 3, centrifugation: Place the centrifuge tube into a special centrifuge device for centrifugal stratification. After centrifugation, the tube is divided into two layers from top to bottom, the upper layer is the PRF layer, and the lower layer is the red blood cell layer;

[0022] Step 4, cutting off red blood cells: moving the rotary cutting structure to the position to be cut between the red blood cell layer and the PRF layer, and rotating the rotary cutting structure to cut off the red blood cell layer;

[0023] Step 5: Rotate the screw cap to release the sealing state between the screw cap and the centrifuge tube body;

[0024] Step 6: Absorbing PRF: Pull the piston rubber stopper of the injection tube body to absorb the component part above the cutting position into the injection tube body to obtain the PRF.

[0025] Compared with the prior art, the present invention has the following effects:

[0026] 1. The present invention integrates the functions of venous whole blood collection, centrifugal separation, red blood cell removal and injection, reducing the need for multiple functional instruments or steps in traditional methods, so that venous whole blood can be directly completed from blood collection to PRF extraction through the device, thereby greatly improving the convenience and flexibility of use;

[0027] 2. In the present invention, the sealed connection design among the centrifuge tube body, the injection tube body, the piston tube and the rotary cutting member can effectively avoid the risk of external pollutants entering, thus ensuring that the PRF preparation process is always in a sterile environment, and further improving the quality and stability of the final product. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 is a schematic structural diagram of a device for preparing platelet-rich fibrin according to the present invention;

[0029] Figure 2 is an exploded view of a device for preparing platelet-rich fibrin according to the present invention;

[0030] Figure 3 is a sectional view of a device for preparing platelet-rich fibrin according to the present invention;

[0031] Figure 4 is Figure 3 an enlarged view of part A in

[0032] Figure 5 is Figure 3 an enlarged view of part B in

[0033] Figure 6 is Figure 3 an enlarged view of part C in

[0034] Figure 7 is Figure 3 an enlarged view of part D in

[0035] Figure 8 is a partially enlarged view of a device for preparing platelet-rich fibrin according to the present invention;

[0036] Figure 9 is a partial schematic view of a device for preparing platelet-rich fibrin according to the present invention.

[0037] In the figure:

[0038] 1. Centrifuge tube body, 2. Piston tube, 3. Injection tube body, 4. Blood collection member, 5. Rotary connection cap, 6. Rotary cutting structure, 7. Braking member,

[0039] 11. First pipe connection member, 12. Through cavity,

[0040] 21. First piston rubber stopper, 22. Slide block, 23. Second pipe connection member, 24. Through port, 25. Threaded groove, 26. Stopping member,

[0041] 261. Stopping surface, 262. Inclined surface, 263. Clamping block, 264. Guide arc,

[0042] 31. Push rod, 32. Third pipe fitting, 33. Flow passage, 34. Injection cavity, 35. Second piston rubber stopper, 36. Rubber soft stopper

[0043] 41. Connector, 42. Limit ring, 43. Blood collection tube, 44. Blood collection needle, 45. Holding part

[0044] 51. Cap

[0045] 61. Control cap, 62. Cutting tool, 63. Control rod

[0046] 71. Support rod, 72. First locking nut, 73. Second locking nut, 74. Engaging part

[0047] 741. Contact surface, 742. Guide groove, 743. Card slot

[0048] 81. Sealing gasket, 82. Sealing ring Detailed implementation mode

[0049] Detailed implementation mode one: Combine Figures 1 to 9 The technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0050] It should be noted that the descriptions of the present invention regarding directions such as "front", "rear", "left", "right", "inside", "outside", "left side", "right side", "upper part", "lower part", "top", "bottom", etc. are all defined based on the orientation or position relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the described structure must be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation to the present invention. In the description of the present invention, the meaning of "a plurality" is more than two, unless otherwise specifically defined.

[0051] In the description of the present invention, unless otherwise clearly specified and limited, the terms "installation", "connection", and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0052] A preparation device for platelet-rich fibrin, comprising a centrifuge tube body 1, a piston tube 2, an injection tube group, a blood collection member 4 and a rotary cutting member. Among them, the injection tube group, the piston tube 2 and the centrifuge tube body 1 are coaxially arranged in sequence from inside to outside. The piston tube 2 is slidably connected to the centrifuge tube body 1. The injection tube group includes an injection tube body 3 and a push rod 31 that is coaxially and axially slidably inserted into the injection tube body 3. The bottom of the injection tube body 3 is detachably connected to the piston tube 2. Sealing settings are provided between the outer wall of the bottom of the push rod 31 and the inner wall of the injection tube body 3, between the bottom of the injection tube body 3 and the bottom of the piston tube 2, and between the outer wall of the bottom of the piston tube 2 and the inner wall of the centrifuge tube body 1. A circulation channel 33 is axially opened inside the push rod 31. An injection cavity 34 is formed inside the injection tube body 3 below the push rod 31. A through cavity 12 is formed inside the centrifuge tube body 1 below the piston tube 2. The circulation channel 33, the injection cavity 34 and the through cavity 12 are axially connected;

[0053] The blood collection member 4 is detachably connected to the push rod 31. When the blood collection member 4 is connected to the push rod 31, the blood collection member 4 is in communication with the circulation channel 33;

[0054] The rotary cutting member is sleeved on the lower opening position of the centrifuge tube body 1 in a relatively movable manner, and a sealing structure is provided between the rotary cutting member and the centrifuge tube body 1.

[0055] The centrifuge tube body 1 is a hollow tubular structure with upper and lower openings.

[0056] The piston tube 2 has a first piston rubber plug 21, and the first piston rubber plug 21 is in sealed dynamic connection with the inner wall of the centrifuge tube body 1.

[0057] The injection tube body 3 and the push rod 31 are both connected to the through cavity 12 at the lower part of the centrifuge tube body 1 to provide negative pressure by removing the air in the through cavity 12. The circulation channel 33 axially penetrates the injection tube body 3 to constitute an inlet for guiding whole blood to flow in.

[0058] A sealing structure is provided between the rotary cutting member and the centrifuge tube body 1 for realizing the sealed connection between the rotary cutting member and the centrifuge tube body.

[0059] The total length of the tube body of the injection tube body 3 is set to 10 - 15 cm and can be used as a syringe to suck PRF and supernatant into the tube body of the injection tube body 3 for surgical use. Among them, the centrifuge tube body 1, the piston tube 2 and the injection tube body 3 that are adapted to the injection tube body 3 are correspondingly sized, so as to realize the whole process from venous blood whole blood collection, centrifugal separation, red blood cell excision to injection, thus realizing the whole process from blood collection to PRF extraction.

[0060] After the centrifuge tube body 1 is assembled with the piston tube 2 and the injection tube body 3, the first piston rubber plug 21 of the piston tube 2 is hermetically connected to the centrifuge tube body 1, and the injection tube body 3 is hermetically connected to the piston tube 2, so that the through cavity 12 of the centrifuge tube body 1 is in a sealed state, thereby forming a relatively closed space among the injection tube body 3, the piston tube 2 and the centrifuge tube body 1. By moving the injection tube body 3 to drive the piston tube 2 to move along the tube direction of the centrifuge tube body 1, the first piston rubber plug 21 of the piston tube 2 is driven to move towards the upper opening direction of the centrifuge tube body 1, the space in the through cavity 12 becomes larger, the pressure in the centrifuge tube body 1 decreases, and the external atmospheric pressure is relatively higher, so as to form a negative pressure in the centrifuge tube body 1.

[0061] In another alternative embodiment, during the manufacture of the device, the gas in the device is removed by means of an external vacuum connector to generate a negative pressure in the through cavity 12, so that the device can realize the function of negative pressure blood collection.

[0062] The present invention integrates the functions of venous whole blood collection, centrifugal separation, red blood cell removal and injection into one, reduces the operation requirements of using multiple functional instruments or steps in the traditional method, enables the venous whole blood to directly complete the whole process from blood collection to PRF extraction through this device, and thus greatly improves the convenience and flexibility of use;

[0063] In the present invention, the sealed connection design among the centrifuge tube body, the injection tube body, the piston tube and the rotary cutting member can effectively avoid the risk of external pollutants entering, thereby ensuring that the PRF preparation process is always in a sterile environment, and further improving the quality and stability of the final product.

[0064] The rotary cutting member includes a rotary connection cap 5 and a rotary cutting structure 6. The rotary connection cap 5 is detachably connected to the bottom of the centrifugal tube body 1. The rotary cutting structure 6 is mounted on the rotary connection cap 5, and the rotary cutting end of the rotary cutting structure 6 is located in the through cavity 12. With such a design, the rotary cutting structure is movably arranged relative to the rotary connection cap, that is, the rotary cutting structure can rotate circumferentially and / or move axially relative to the rotary connection cap. In this embodiment, after centrifuging the whole blood, it is divided into two layers. The upper layer is the PRF layer, and the lower layer is the red blood cell layer. The rotary cutting member is used to cut off the red blood cell layer. When the blood collection member 4 collects the whole blood, the rotary cutting structure 6 is moved to the initial position (the bottom end of the through cavity 12). After the whole blood is separated, the rotary connection cap 5 and the rotary cutting structure 6 are appropriately adjusted according to the position of the red blood cell layer, and the rotary cutting structure 6 is rotated to separate the lower red blood cell layer from the middle PRF layer, so as to realize the cutting operation of the red blood cell layer. In this embodiment, the rotary connection cap 5 and the lower opening of the centrifugal tube body 1 are set to be screwed. The rotary connection cap 5 is provided with a cap plug 51, and the cap plug 51 protrudes into the through cavity 12. The sealing structure is arranged at the connection between the cap plug 51 and the through cavity 12. The sealing structure includes a sealing ring 82 arranged at the connection between the cap plug 51 and the through cavity 12. The inner ring of the sealing ring 82 abuts against the outer wall of the cap plug 51. The sealing ring 82 is used to realize the sealing function between the rotary connection cap 5 and the centrifugal tube body 1 in the initial state. When it is necessary to cut off the red blood cell layer, the rotary connection cap 5 can rotate relative to the centrifugal tube body 1, so as to adjust the distance between the rotary connection cap 5 and the lower opening of the centrifugal tube body 1, and further realize the adjustment of the position of the rotary connection cap 5.

[0065] The rotary cutting structure 6 includes a control rod 63 and a cutting tool 62 installed at one end of the control rod 63. The cutting tool 62 is located in the through cavity 12, and the control rod 63 is threadedly connected to the rotary connection cap 5. With such a design, the control rod rotates under the action of an external force to drive the cutting tool to rotate and move along the axial direction of the centrifugal tube body. The control rod 63 is screwed to the rotary connection cap 5. By rotating the control rod 63, the cutting tool 62 can be driven to move along the tube direction of the centrifugal tube body 1, and further realize the adjustment of the position of the cutting tool 62. Exemplarily, a plurality of cutting tools 62 are provided and evenly distributed along the circumferential direction of the control rod 63. The cutting tool 62 can be selected as a medical steel wire, which has good support. During specific operation, the cutting tool 62 is moved to the boundary line between the red blood cell layer and the PRF layer, and the control rod 63 is rotated to drive the cutting tool 62 to rotate, so as to separate the red blood cell layer from the PRF layer, thus realizing the cutting operation of the red blood cell layer. The cut red blood cell layer remains in the through cavity 12. The push rod 31 is pulled to suck the supernatant and PRF into the injection cavity 34 for subsequent surgical use.

[0066] In this embodiment, a control cap 61 is provided at the bottom of the control rod 63, and the control cap 61 is used to control the rotation of the control rod 63. The sealing structure further includes a sealing gasket 81, and the sealing gasket 81 is clamped between the rotary connection cap 5 and the control cap 61, so as to realize the sealing at the screwed connection between the control rod 63 and the rotary connection cap 5.

[0067] The screw-on cap 5 is threadedly connected to the bottom of the centrifuge tube body 1. A cap plug 51 is provided on the screw-on cap 5, and the cap plug 51 protrudes into the through cavity 12. Sealing structures are provided both between the cap plug 51 and the inner wall of the centrifuge tube body 1 and between the outer wall of the centrifuge tube body 1 and the screw-on cap 5.

[0068] A slider 22 is provided on the outer wall of the piston tube 2, and a sliding groove adapted to the slider 22 is correspondingly provided on the inner wall of the centrifuge tube body 1. Designed in this way, the slider 22 is adapted to the sliding groove, so that the piston tube 2 slides linearly along the tube body of the centrifuge tube body 1, thus avoiding relative rotation between the piston tube 2 and the centrifuge tube body 1.

[0069] The top of the push rod 31 is blocked with a rubber soft plug 36. Designed in this way, the rubber soft plug is used to block the inlet to cut off the communication channel from the outside. The rubber soft plug 36 is made of synthetic rubber materials such as medical-grade butyl rubber or neoprene, which has elasticity and sealing performance, so as to realize the sealing of the inlet of the communication channel 33, and further ensure the connection sealing performance of the centrifuge tube body 1, the piston tube 2, and the injection tube body 3.

[0070] The bottom of the injection tube body 3 is processed with a threaded joint. A first piston rubber plug 21 is provided at the bottom of the piston tube 2. The first piston rubber plug 21 is provided with a threaded groove 25 that matches the shape of the threaded joint, and the threaded groove 25 is adapted to the threaded joint. Designed in this way, the first piston rubber plug 21 is made of synthetic rubber materials such as medical-grade butyl rubber or neoprene, which has elasticity and sealing performance. A threaded groove 25 that cooperates with the threaded joint is formed on the first piston rubber plug 21. The threaded joint is inserted into the threaded groove 25, and then the injection tube body 3 is rotated to be screwed with the threaded groove 25. Due to the connection between the slider 22 and the sliding groove restricting the rotation function of the piston tube 2, when the threaded joint is screwed with the threaded groove 25, the connection between the injection tube body 3 and the piston tube 2 can be realized without controlling the piston tube 2. The first piston rubber plug 21 has a through port 24, and the through port 24 is axially opened on the first piston rubber plug 21. The through port 24 is used to realize the conduction function of the centrifuge tube body 1, the injection tube body 3, and the piston tube 2.

[0071] The bottom outer wall of the push rod and the inner wall of the injection tube body are hermetically connected by a second piston rubber seal. Designed in this way, the second piston rubber plug 35 is dynamically hermetically connected to the inner wall of the injection tube body 3, and the push rod 31 is used to push the second piston rubber plug 35 to move axially along the injection tube body 3. When the push rod 31 drives the second piston rubber plug 35 to move towards the upper opening of the centrifuge tube body 1, the injection tube body 3 draws the liquid in the through cavity 12 into the injection cavity 34, thus realizing the liquid taking function of the injection tube body 3.

[0072] The preparation device further includes a braking member 7. The braking member 7 includes a support rod 71 hinged to the centrifuge tube body 1, and a first locking nut 72 and a second locking nut 73 respectively screwed to the support rod 71. A first pipe connector 11 is provided at the upper opening of the centrifuge tube body 1. The bottom end of the support rod 71 is rotatably connected to the first pipe connector 11. A third pipe connector 32 is provided at the top end of the injection tube body 3. An opening groove for the support rod 71 to penetrate through is formed in the third pipe connector 32. The opening groove is of a U-shaped structure, and the groove edge is smoothly transitioned with the outer periphery of the third pipe connector 32. By screwing the first locking nut 72 and the second locking nut 73 to the support rod 71, the third pipe connector 32 is clamped. During specific operation, medical staff determines the moving distance of the injection tube body 3 according to the required sample volume, so that the space in the through cavity 12 can accommodate the required sample. Then, the injection tube body 3 is moved, so that the space in the through cavity 12 gradually increases. The position of the injection tube body 3 is determined according to the scale line on the centrifuge tube body 1. When reaching the preset position, the support rod 71 is rotated, so that the top end of the support rod 71 moves towards the opening groove. The support rod 71 enters the opening groove. After the second locking nut 73 is screwed to the support rod 71, the first locking nut 72 is then screwed to the support rod 71, thereby realizing the position fixation of the injection tube body 1, and further avoiding the relative displacement between the injection tube body 3 and the centrifuge tube body 1. By screwing the first locking nut 72 and the second locking nut 73 to the support rod 71, the friction force between the first locking nut 72, the second locking nut 73 and the third pipe connector 32 can be increased, and an additional pressure is applied to ensure the fastening effect between the injection tube body 1 and the centrifuge tube body 1.

[0073] Exemplarily, in combination with Figure 8 as shown, a second pipe connector 23 is provided at the top end of the piston tube 2. An avoidance groove for the support rod 71 to penetrate through is formed in the second pipe connector 23. The support rod 71 can be received in the avoidance groove. A stop member 26 is provided on the groove wall of the avoidance groove. The support rod 71 is provided with an engaging member 74 cooperating with the stop member 26. After the support rod 71 enters the avoidance groove, the stop member 26 and the engaging member 74 are elastically clamped to realize the braking function of the second pipe connector 23, thereby realizing the position fixation of the piston tube 2.

[0074] Specifically, when the stop member 26 and the engaging member 74 cooperate, the stop surface 261 of the stop member 26 abuts against the abutting surface 741 of the engaging member 74. Both the stop surface 261 and the abutting surface 741 are inclined surfaces. A clamping block 263 is provided on the stop member 26. The clamping block 263 has elasticity. The engaging member 74 is provided with a clamping groove 743 corresponding to the clamping block 263 and a guiding groove 742 for guiding the clamping block 263 into the clamping groove 743. The clamping block 263 is elastically snapped into the guiding groove 742, thereby realizing the braking function of the second pipe connector 23, and thereby realizing the position fixation of the piston tube 2.

[0075] The number of engaging members 74 on the support rod 71 can be one or more. When there are multiple engaging members 74, the multiple engaging members 74 are distributed along the length direction of the support rod 71, so as to achieve the engagement between the engaging member 74 at the corresponding position and the stopper 26 after the piston tube 2 is axially displaced and adjusted to different positions.

[0076] In this embodiment, when the clamping block 263 moves along the guide groove 742, a guiding arc 264 is formed at the contact point between the clamping block 263 and the guide groove 742. The guiding arc 264 is used to guide the clamping block 263 into the card slot 743. The stopper 26 also has an inclined surface 262. The setting of the inclined surface 262 can avoid direct contact with the support rod 71, thereby avoiding blocking the support rod 71.

[0077] The present invention moves the injection tube body according to the required sample volume, so as to form a space for storing the sample between the injection tube body and the centrifuge tube body. The braking member is used to relatively fix the positions of the injection tube body and the centrifuge tube body, thereby ensuring the stability between the injection tube body and the centrifuge tube body.

[0078] The blood collection member 4 includes a blood collection needle 44, a blood collection tube 43 and a connector 41 that are connected in sequence from head to tail. Among them, the connector 41 is detachably connected to the push rod 31. When the connector 41 is connected to the push rod 31, the inside of the connector 41 is communicated with the flow channel 33. Designed in this way, the blood collection needle pierces the patient's venous blood vessel, and the whole blood is sequentially introduced into the centrifuge tube body through the blood collection tube, the connector and the flow channel. The connector 41 has a tip. With the help of the tip, the connector 41 can easily pierce the rubber soft plug 36 and make the connector 41 enter the flow channel 33. A limit ring 42 is axially provided at the top of the connector 41. After the connector 41 pierces the rubber soft plug 36, the limit ring 42 abuts against the rubber soft plug 36, thereby preventing the connector 41 from completely entering the flow channel 33. The limit ring 42 can also facilitate the removal of the connector 41 from the push rod 31.

[0079] A plurality of shunt holes are provided on the connector, and all the shunt holes are communicated with the flow channel. Designed in this way, the whole blood collected by the blood collection needle is introduced into the cavity of the centrifuge tube body through the shunt holes. The connector 41 is a tubular structure. The plurality of shunt holes are arranged at equal intervals along the circumferential direction of the connector 41. A diversion cavity communicated with the blood collection tube 43 is formed inside the connector 41. The diversion cavity is communicated with the shunt holes. The whole blood collected by the blood collection needle 44 flows into the blood collection tube 43 and the diversion cavity in sequence, and flows into the flow channel 33 from the plurality of shunt holes. Finally, the whole blood flows into the cavity 12, thereby realizing the blood collection function, and the circumferentially opened shunt holes can make the whole blood be introduced into the cavity 12 relatively evenly.

[0080] During specific application, the blood collection needle 44 pierces the patient's skin and is placed in the vein. Since the centrifuge tube body 1 is in a negative pressure state, the whole venous blood is collected by the blood collection needle 44 and flows into the blood collection tube 43 and the diversion cavity in turn. The whole blood in the diversion cavity flows into the circulation channel 33 through the shunt hole, and finally the whole blood flows into the through cavity 12, thereby realizing the blood collection function.

[0081] In this embodiment, a hand-held portion 45 is provided on the blood collection needle 44, which can be easily operated by medical personnel, and a protective shell is provided on the blood collection needle 44, which makes the blood collection needle 44 in a sealed state, thereby ensuring the safety of the blood collection needle 44.

[0082] A method for preparing platelet-rich fibrin, using any of the above-mentioned preparation devices, comprises the following steps:

[0083] Step 1, device preparation: move the rotary cutting structure 6 to a position adjacent to the lower opening of the centrifuge tube body 1 (this position is the initial position of the rotary cutting structure 6, at which time the cutter 62 is located near the lower opening at the bottom of the centrifuge tube body 1), pull the injection tube body 3 to form a negative pressure, so that the venous whole blood can enter the centrifuge tube body 1 during blood collection;

[0084] Step 2, collecting blood: insert the blood sampling member 4 into the patient's vein, and after confirming the blood return, connect the blood sampling member 4 with the top of the push rod 31, so that the venous blood is introduced into the centrifuge tube body 1 from the blood sampling member 4;

[0085] Step 3, centrifugation: placing the centrifuge tube 1 into a special centrifuge device for centrifugal stratification, and separating the centrifuge tube into two layers from top to bottom after centrifugation, the upper layer is the PRF layer, and the lower layer is the red blood cell layer;

[0086] Step 4, excising red blood cells: moving the rotary cutting structure 6 to the position to be cut between the red blood cell layer and the PRF layer (the position to be cut is the position where the complete PRF and a small amount of red blood cells are retained after excision), and rotating the rotary cutting structure 6 to cut off the red blood cell layer (separating the red blood cell layer from the PRF layer);

[0087] Step 5: Rotate the screw cap 5 to release the sealing state between the screw cap 5 and the centrifuge tube body 1;

[0088] Step 6: Absorbing PRF: Pull the piston rubber plug of the injection tube body 3 to absorb the component part above the cutting position into the injection tube body 3 to obtain the PRF.

[0089] In some operation scenarios, PRF may precipitate some plasma, but it will not affect the treatment.

[0090] The above are only the preferred specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, making equivalent substitutions or changes should be covered within the protection scope of the present invention.

Claims

1. An apparatus for preparing platelet-rich fibrin, characterized in that: It includes a centrifuge tube body (1), a piston tube (2), an injection tube group, a blood collection component (4) and a rotary cutting component. Among them, the injection tube group, the piston tube (2) and the centrifuge tube body (1) are coaxially arranged in sequence from inside to outside. The piston tube (2) is slidably connected to the centrifuge tube body (1). The injection tube group includes an injection tube body (3) and a push rod (31) that is coaxially and axially slidably inserted into the injection tube body (3). The bottom of the injection tube body (3) is detachably connected to the piston tube (2). Sealing is provided between the outer wall of the bottom of the push rod (31) and the inner wall of the injection tube body (3), between the bottom of the injection tube body (3) and the bottom of the piston tube (2), and between the outer wall of the bottom of the piston tube (2) and the inner wall of the centrifuge tube body (1). An axial flow channel (33) is opened inside the push rod (31). An injection cavity (34) is formed inside the injection tube body (3) below the push rod (31). A through cavity (12) is formed inside the centrifuge tube body (1) below the piston tube (2). The flow channel (33), the injection cavity (34) and the through cavity (12) are axially connected; The blood collection component (4) is detachably connected to the push rod (31). When the blood collection component (4) is connected to the push rod (31), the blood collection component (4) is in communication with the flow channel (33); The rotary cutting component is sleeved on the lower opening position of the centrifuge tube body (1) in a relatively movable manner, and a sealing structure is provided between the rotary cutting component and the centrifuge tube body (1).

2. The preparation device of platelet-rich fibrin according to claim 1, wherein: The rotary cutting component includes a rotary connection cap (5) and a rotary cutting structure (6). The rotary connection cap (5) is detachably connected to the bottom of the centrifuge tube body (1). The rotary cutting structure (6) is inserted into the rotary connection cap (5), and the rotary cutting end of the rotary cutting structure (6) is located in the through cavity (12).

3. The preparation device of platelet-rich fibrin according to claim 2, characterized in that: The rotary cutting structure (6) includes a control rod (63) and a cutting tool (62) installed at one end of the control rod (63). The cutting tool (62) is located in the through cavity (12), and the control rod (63) is threadedly connected to the rotary connection cap (5).

4. The preparation device of platelet-rich fibrin according to claim 2, wherein: The rotary connection cap (5) is threadedly connected to the bottom of the centrifuge tube body (1). A cap plug (51) is provided on the rotary connection cap (5), and the cap plug (51) protrudes into the through cavity (12). Sealing structures are provided between the cap plug (51) and the inner wall of the centrifuge tube body (1) and between the outer wall of the centrifuge tube body (1) and the rotary connection cap (5).

5. The preparation device of platelet-rich fibrin according to claim 2, characterized in that: Sliders (22) are provided on the outer wall of the piston tube (2), and corresponding chutes for cooperating with the sliders (22) are provided on the inner wall of the centrifuge tube body (1).

6. The preparation device of platelet-rich fibrin according to claim 2, characterized in that: The top of the push rod (31) is blocked with a rubber soft plug (36).

7. The preparation device of platelet-rich fibrin according to claim 2, characterized in that: The bottom of the injection tube body (3) is processed with a threaded joint. A first piston rubber plug (21) is provided at the bottom of the piston tube (2). The first piston rubber plug (21) is provided with a threaded groove (25) that matches the shape of the threaded joint, and the threaded groove (25) is adapted to the threaded joint.

8. The preparation device of platelet-rich fibrin according to claim 2, characterized in that: The preparation device also includes a braking component (7), which includes a support rod (71) hinged to the centrifuge tube body (1) and a first locking nut (72) and a second locking nut (73) respectively screwed to the support rod (71); the upper opening of the centrifuge tube body (1) is provided with a first pipe connector (11); the bottom end of the support rod (71) is rotatably connected to the first pipe connector (11); the top end of the injection tube body (3) is provided with a third pipe connector (32); the third pipe connector (32) is provided with an open groove for the support rod (71) to pass through; the open groove is a U-shaped structure, and the groove edge is smoothly transitioned to the outer circumference of the third pipe connector (32); the first locking nut (72) and the second locking nut (73) are screwed to the support rod (71) to clamp the third pipe connector (32).

9. The preparation device of platelet-rich fibrin according to claim 2, characterized in that: The blood sampling component (4) comprises a blood sampling needle (44), a blood sampling tube (43) and a connector (41) which are connected end to end in sequence, wherein the connector (41) is detachably connected to the push rod (31), and when the connector (41) is connected to the push rod (31), the interior of the connector (41) is connected to the circulation channel (33).

10. A method for preparing platelet-rich fibrin, characterized in that: The preparation device according to any one of claims 1 to 9 comprises the following steps: Step 1, device preparation: move the rotary cutting structure (6) to a position adjacent to the lower opening of the centrifuge tube body (1), pull the injection tube body (3) to form negative pressure, so that venous whole blood can enter the centrifuge tube body (1) during blood collection; Step 2, collecting blood: inserting the blood sampling member (4) into the patient's vein, and after confirming the blood return, connecting the blood sampling member (4) with the top of the push rod (31), so that the venous blood is introduced into the centrifuge tube body (1) from the blood sampling member (4); Step 3, centrifugation: placing the centrifuge tube (1) into a dedicated centrifuge device for centrifugal stratification, and separating the centrifuge tube (1) into two layers from top to bottom after centrifugation, the upper layer being the PRF layer and the lower layer being the red blood cell layer; Step 4, cutting off red blood cells: moving the rotary cutting structure (6) to the position to be cut between the red blood cell layer and the PRF layer, and rotating the rotary cutting structure (6) to cut off the red blood cell layer; Step 5: Rotate the screw cap (5) to release the sealing state between the screw cap (5) and the centrifuge tube body (1); Step 6: Absorbing PRF: Pull the piston rubber plug of the injection tube body (3) to absorb the component part above the cutting position into the injection tube body (3) to obtain the PRF.

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