Perfusion device for extracting placenta stem cells

Abstract

The invention belongs to the technical field of biomedicine, particularly relates to a perfusion device for extracting placenta stem cells, and aims to overcome the defects that in the prior art, when the stem cells are extracted, embryonic tissues cannot be thoroughly cut, and when the stem cells are collected, impurities are easily attached to filter cloth for collecting the stem cells. Comprising a base, a separation box is fixedly installed on one side of the top of the base, a cover plate is installed on the separation box in a clamped mode, four electric push rods are symmetrically and fixedly installed on the top of the base, output shafts of the electric push rods are fixedly connected with the cover plate, and a smashing box is fixedly installed on the inner wall of the top of the cover plate in a penetrating mode. According to the stem cell extraction device, the embryonic tissue can be cut, ground and crushed in advance, so that stem cells can be thoroughly separated out, and the stem cells can be subjected to multi-stage filtration, so that impurities can be reduced when the stem cells are extracted, and the stem cell extraction device has good usability.

Description

Technical Field

[0001] The present invention relates to the field of biomedical technologies, and particularly to a perfusion device for extracting placental stem cells. Background Art

[0002] Placental stem cells are stem cells obtained by collecting placental tissues. Placenta-derived stem cells play a crucial role in the clinical application prospects and have infinite prospects in multiple fields such as autologous organ repair, cloning transplantation, beauty, treatment of genetic diseases, and heart diseases, liver diseases, diabetes, etc.

[0003] Currently, the corresponding placental stem cells are mainly extracted by perfusion. During the extraction process, the perfusion device used for extracting stem cells still has the following deficiencies in actual use:

[0004] 1. When comminuting embryonic tissues, the cutting and comminuting method is often used to comminute embryonic tissues. However, in actual use, generally when cutting embryonic tissues, it is difficult to achieve complete differentiation of embryonic tissues. Therefore, when separating stem cells from embryonic tissues, it is difficult to achieve complete separation.

[0005] 2. Moreover, when filtering stem cells during the perfusion process, the cut embryonic tissues are often left on the filter cloth for collecting stem cells, resulting in impurities often adhering to the filter cloth when collecting stem cells.

[0006] In view of the above problems, the present invention document proposes a perfusion device for extracting placental stem cells. Summary of the Invention

[0007] The present invention provides a perfusion device for extracting placental stem cells, which solves the drawbacks in the prior art that when extracting stem cells, complete cutting of embryonic tissues cannot be achieved and impurities are likely to adhere to the filter cloth for collecting stem cells.

[0008] The present invention provides the following technical solutions:

[0009] A perfusion device for extracting placental stem cells includes a base. On one side of the top of the base, a separation box is fixedly installed. A cover plate is clamped on the separation box. Four electric push rods are symmetrically and fixedly installed on the top of the base. The output shaft of the electric push rod is fixedly connected to the cover plate. A comminuting box is fixedly installed through the inner wall of the top of the cover plate. A feeding box is fixedly installed on the inner wall of the top of the comminuting box. The top of the feeding box extends above the comminuting box. A feeding pipe is fixedly installed through one inner wall of the feeding box. The perfusion device further includes:

[0010] Cutting mechanism, the cutting mechanism is installed on the inner wall of the top of the feeding box, the bottom of the cutting mechanism extends into the crushing box and is connected to the inner wall of the crushing box, and the cutting mechanism is used for crushing and cutting embryonic tissues;

[0011] Grinding mechanism, the grinding mechanism is installed in the crushing box, the top of the grinding mechanism is connected to the cutting mechanism, and the bottom of the grinding mechanism extends to the outside of the separation box and is connected to the outside of the separation box;

[0012] Filtering mechanism, the filtering mechanism is installed on the other side of the top of the base, one side of the filtering mechanism extends into the separation box and is connected to the grinding mechanism, and the top of the grinding mechanism extends into the feeding box and is connected to the inner wall of the other side of the feeding box.

[0013] In a possible design, the cutting mechanism includes a mounting frame rotatably connected in the crushing box, a plurality of blanking holes are equidistantly arranged on the mounting frame, a conical disk is fixedly installed in the mounting frame, the grinding mechanism is connected to the bottom of the conical disk, a transmission component is installed on the top of the conical disk, a moving frame is connected to the transmission component, a plurality of separating cutters are fixedly installed at equal intervals at the bottom of the moving frame, the separating cutters correspond to the corresponding blanking holes, and the top end of the transmission component extends into the feeding box and is connected to the inner wall of the center of the top of the feeding box.

[0014] In a possible design, the transmission component includes two limiting rods symmetrically and fixedly installed on the top of the conical disk, the same limiting plate is slidably connected on the two limiting rods, the limiting plate is fixedly connected to the inner wall of the moving frame, an installation rod is fixedly installed on the inner wall of the center of the top of the feeding box, the bottom end of the installation rod extends into the crushing box and is fixedly installed with a ball screw, a sliding ring matched with the spiral groove on the ball screw is arranged on the ball screw, and the bottom end of the ball screw penetrates through the limiting plate and is rotatably connected to the top of the conical disk.

[0015] In a possible design, a connecting plate is fixedly installed at the top end of the limiting rod, and the same separating ring is fixedly installed on the two connecting plates, and the separating ring is rotatably connected to the inner wall of the feeding box.

[0016] In a possible design, the grinding mechanism includes a conical grinding disk fixedly installed at the center position of the bottom of the conical disk, and a grinding ring is fixedly installed on the inner wall of the crushing box, the bottom of the conical grinding disk extends into the grinding ring and is matched with the inner wall of the grinding ring, a driving motor is fixedly installed at the top of one side of the separation box, the output shaft of the driving motor extends into the separation box and is fixedly installed with a driving bevel gear, and a bevel gear ring is fixedly installed at the bottom of the conical grinding disk, the driving bevel gear is meshed with the bevel gear ring, and a separating stirring rod is fixedly installed at the center position of the bottom of the conical grinding disk, and the bottom of the separating stirring rod extends into the separation box.

[0017] In a possible design, the grinding mechanism further includes an annular mesh cover. A first magnet ring is fixedly installed at the bottom of the grinding ring, and a second magnet ring is fixedly sleeved on the annular mesh cover. The second magnet ring is attracted to the first magnet ring.

[0018] In a possible design, the filtering mechanism includes a collection box fixedly installed on the other side of the top of the base. A primary filter plate, a secondary filter plate, and a biofilm filter plate are sequentially and movably clamped in the collection box. The tops of the primary filter plate, the secondary filter plate, and the biofilm filter plate all extend above the collection box. A first attracting component is installed on the inner wall of one side of the top of the collection box. One side of the first attracting component extends into the separation box and is connected to the inner wall of one side of the separation box. The first attracting component cooperates with the bevel gear ring. A second attracting component is installed through the other side of the top of the collection box. The top of the second attracting component extends into the feeding box and is connected to the inner wall of the other side of the feeding box. The bottom of the second attracting component penetrates through the cover plate and extends into the separation box. The bottom of the second attracting component is connected to the first attracting component.

[0019] In a possible design, the first attracting component includes a first power box fixedly installed on the inner wall of one side of the separation box. One side of the first power box extends to the outside of the separation box. A transmission rod is rotatably connected through the inner wall of one side of the first power box. One end of the transmission rod is fixedly installed with a first water absorbing blade. One end of the transmission rod extends into the separation box and is fixedly installed with a driven bevel gear. The driven bevel gear meshes with the bevel gear ring. A suction pipe is fixedly installed on the inner wall of the bottom of the first power box. The bottom end of the suction pipe extends into the separation box and is fixedly connected to the inner wall of the bottom of one side of the separation box. A liquid outlet pipe is fixedly installed on the inner wall of the other side of the first power box. One end of the liquid outlet pipe extends to the outside of the first power box and is fixedly installed with a conveying cover. The bottom of the conveying cover extends into the collection box and is fixedly connected to the inner wall of one side of the top of the collection box.

[0020] In a possible design, the second attracting component includes a hose that penetrates through the inner wall of the other side of the top of the collection box and is fixedly connected to the inner wall of the other side of the collection box. An installation pipe is fixedly installed on the inner wall of the other side of the feeding box. One end of the installation pipe extends to the outside of the feeding box and is fixedly installed with a second power box. The top end of the hose extends into the second power box and is fixedly connected to the inner wall of the other side of the second power box. A blade component is connected through the inner wall of the bottom of the second power box. The bottom of the blade component penetrates through the cover plate and extends into the separation box. The blade component is connected to the transmission rod.

[0021] In a possible design, the blade member includes a rotating shaft that penetrates through the inner wall of the bottom of the second power box and is rotatably connected to the inner wall of the bottom of the second power box. A second water-absorbing blade is fixedly installed at the top end of the rotating shaft. The bottom end of the rotating shaft penetrates through the cover plate and extends into the separation box. A driving bevel gear is fixedly installed at the bottom end of the rotating shaft. A connecting bevel gear located in the separation box is fixedly sleeved on the transmission rod, and the connecting bevel gear meshes with the driving bevel gear.

[0022] In the present invention, first, the water used for extracting stem cells is injected into the separation box through the feeding pipe. Then, the driving motor can be started to drive the driving bevel gear to rotate. At this time, under the meshing transmission with the bevel gear ring, the conical grinding disc can be driven to rotate. When the conical disc rotates, two limit rods can be driven to perform circular motion, thereby driving the limit plate to rotate. When the limit plate rotates, the slip ring can be driven to rotate. Thus, under the transmission cooperation with the ball screw, the limit plate can be driven to perform longitudinal reciprocating motion, which can drive the moving frame to perform longitudinal reciprocating motion, and then drive a plurality of separation cutters to perform longitudinal reciprocating motion. At this time, after the embryonic tissue is put into the feeding box through the feeding pipe, the embryonic tissue can fall on the conical disc, and then the embryonic tissue can slide onto the mounting frame through the conical disc. Therefore, a plurality of separation cutters can be used to cut the embryonic tissue, and the embryonic tissue is cut into small pieces and falls on the conical grinding disc through the feeding hole. And the conical grinding disc keeps rotating, which can generate centrifugal force on the embryonic assembly, making the embryonic tissue move towards the edge of the conical grinding disc. Then, with the cooperation of the grinding ring, the embryonic tissue can be ground and crushed, so that the stem cells on the embryonic tissue can be completely separated. The embryonic tissue after grinding and crushing and the separated stem cells can fall into the annular wire mesh cover. And when the bevel gear ring rotates, under the meshing transmission with the driven bevel gear, the transmission rod can be driven to rotate. When the transmission rod rotates, the first water suction blade can be driven to rotate. When the first water suction blade rotates, suction can be generated. At this time, the water mixed with stem cells in the separation box can be sucked into the first power box through the suction pipe, and then the water can be transported to the collection box through the liquid outlet pipe and the conveying cover. Then, with the filtering action of the primary filter plate and the secondary filter plate, the impurities in the water can be filtered. After the water flows through the biological membrane filter plate, the stem cells can be filtered to realize the collection of stem cells. And when the transmission rod rotates, the connecting bevel gear can be driven to rotate. At this time, under the meshing transmission with the driving bevel gear, the rotating shaft can be driven to rotate. When the rotating shaft rotates, the second water suction blade can be driven to rotate, thereby generating suction. In this way, the water in the collection box can be sucked into the second power box through the hose, and then the water can be transported to the feeding box through the installation pipe. That is, when cutting the embryonic tissue, the embryonic tissue can be flushed. As the water flows downward, the embryonic tissue after grinding and crushing can be washed, so that the stem cells can be flushed into the separation box, and thus the stem cells can be completely separated. After the stem cells are extracted, four electric push rods can be started to push the cover plate upward, and then it is convenient to clean the separation box. At the same time, by pulling down the annular wire mesh cover to separate the first magnet ring from the second magnet ring, the embryonic tissue falling in the annular wire mesh cover can be cleaned up.

[0023] It should be understood that the above general description and the following detailed description are merely exemplary and do not limit the present invention.

[0024] Beneficial effects: In the present invention, through the arranged cutting mechanism, after the conical disk receives the power of the grinding mechanism, it can rotate. At this time, it can drive the mounting rack to rotate. And when the conical disk rotates, it can drive the transmission component to move, so as to drive the moving rack to perform longitudinal reciprocating motion, and then drive a plurality of separating cutters to perform longitudinal reciprocating motion, so as to cut the embryo tissue and divide the embryo tissue into small pieces, so as to utilize the grinding mechanism to grind and crush the embryo tissue;

[0025] In the present invention, through the arranged grinding mechanism, by starting the driving motor to drive the driving bevel gear to rotate, at this time, under the meshing transmission with the bevel gear ring, it can drive the conical grinding disk to rotate. Therefore, after the cut embryo tissue falls on the conical grinding disk, under the action of centrifugal force, the embryo tissue can move towards the edge of the conical grinding disk. Then, with the cooperation of the grinding ring, the embryo tissue can be ground and crushed, so that the stem cells on the embryo tissue can be completely separated;

[0026] And the ground embryo tissue will fall into the annular mesh cover. Then, when water flows through the annular mesh cover, it can wash the separated stem cells into the separation box, so that when centrifugally stirring the stem cells, it can prevent the embryo tissue from falling into the separation box, and when extracting the stem cells, it can improve the purity;

[0027] In the present invention, through the arranged filtering mechanism, after the first suction component receives the power of the bevel gear ring, it can operate. At this time, it can generate suction to suck the water mixed with stem cells into the collection box. Then, under the filtering action of the primary filter plate and the secondary filter plate, the impurities in the water can be filtered. After the water flows through the biological membrane filter plate, it can filter the stem cells. And when the first suction component moves, it can drive the second suction component to move, so as to pump the water flowing into the collection box into the feeding box. Then, when cutting the embryo tissue, it can wash the embryo tissue. As the water flows downward, it can wash the ground and crushed embryo tissue, so as to wash the stem cells into the separation box, so that the stem cells can be completely separated.

[0028] When the present invention extracts embryonic stem cells, it can pre-cut and grind and crush the embryo tissue, so that the stem cells can be completely separated, and multi-stage filtration of the stem cells can be realized. Therefore, when extracting the stem cells, impurities can be reduced, so it has good usability. Description of the Drawings

[0029] Figure 1 A three-dimensional schematic diagram of the overall structure of the perfusion device for placental stem cell extraction provided by the embodiment of the present invention;

[0030] Figure 2 A schematic cross-sectional structure diagram of the main structure of the perfusion device for placental stem cell extraction provided by the embodiment of the present invention;

[0031] Figure 3 A three-dimensional schematic cross-sectional structure diagram of the main structure of the perfusion device for placental stem cell extraction provided by the embodiment of the present invention;

[0032] Figure 4 A schematic diagram of the connection structure of the mounting frame, conical disc, grinding disc and grinding ring of the perfusion device for placental stem cell extraction provided by the embodiment of the present invention;

[0033] Figure 5 A three-dimensional schematic diagram of the connection structure of the mounting frame, moving frame and multiple separation cutters of the perfusion device for placental stem cell extraction provided by the embodiment of the present invention;

[0034] Figure 6 A three-dimensional schematic diagram of the connection structure of the drive motor, active bevel gear, driven bevel gear and mounting frame of the perfusion device for placental stem cell extraction provided by the embodiment of the present invention;

[0035] Figure 7 A three-dimensional schematic diagram of the connection structure of the mounting rod, ball screw, moving frame and multiple separation cutters of the perfusion device for placental stem cell extraction provided by the embodiment of the present invention;

[0036] Figure 8 A three-dimensional schematic cross-sectional structure diagram of the collection box of the perfusion device for placental stem cell extraction provided by the embodiment of the present invention;

[0037] Figure 9 A three-dimensional schematic diagram of the structure of the primary filter plate, secondary filter plate and biofilm filter plate of the perfusion device for placental stem cell extraction provided by the embodiment of the present invention.

[0038] Reference numerals:

[0039] 1. Base; 2. Separation box; 3. Cover plate; 4. Electric push rod; 5. Crushing box; 6. Feeding box; 7. Mounting frame; 8. Conical disc; 9. Limiting rod; 10. Limiting plate; 11. Moving frame; 12. Separation cutter; 13. Feeding hole; 14. Conical grinding disc; 15. Grinding ring; 16. Annular mesh cover; 17. First magnet ring; 18. Second magnet ring; 19. Mounting rod; 20. Ball screw; 21. Slip ring; 22. Connecting plate; 23. Separation ring; 24. Feeding pipe; 25. Driving motor; 26. Driving bevel gear; 27. Bevel gear ring; 28. First power box; 29. Transmission rod; 30. Driven bevel gear; 31. Liquid outlet pipe; 32. Conveyor cover; 33. Collection box; 34. Primary filter plate; 35. Secondary filter plate; 36. Biofilm filter plate; 37. Hose; 38. Mounting pipe; 39. Second power box; 40. Rotating shaft; 41. First water suction blade; 42. Connecting bevel gear; 43. Driving bevel gear; 44. Suction pipe; 45. Second water suction blade; 46. Separation stirring rod. Detailed implementation mode

[0040] The embodiments of the present invention will be described below with reference to the accompanying drawings in the embodiments of the present invention.

[0041] In the description of the embodiments of the present invention, it should be noted that unless otherwise clearly specified and limited, the terms "connection" and "installation" should be understood in a broad sense. For example, "connection" can be a detachable connection or a non-detachable connection; it can be a direct connection or an indirect connection through an intermediate medium. In addition, "communication" can be a direct communication or an indirect communication through an intermediate medium. Among them, "fixing" means that they are connected to each other and the relative positional relationship after connection remains unchanged. The orientation terms mentioned in the embodiments of the present invention, such as "inside", "outside", "top", "bottom", etc., are only for reference to the direction of the accompanying drawings. Therefore, the orientation terms used are for better and clearer description and understanding of the embodiments of the present invention, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the embodiments of the present invention.

[0042] In the embodiments of the present invention, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features.

[0043] In the embodiments of the present invention, "and / or" is only a description of the association relationship of the associated objects, indicating that there can be three relationships. For example, A and / or B can represent: A exists alone, A and B exist simultaneously, and B exists alone. In addition, the character " / " in this article generally represents an "or" relationship between the associated objects before and after.

[0044] References to "one embodiment" or "some embodiments" or the like described in this specification mean that a particular feature, structure, or characteristic described in connection with the embodiment is included in one or more embodiments of the present invention. Thus, statements such as "in one embodiment", "in some embodiments", "in other some embodiments", "in still other embodiments", etc. that appear in different places in this specification do not necessarily all refer to the same embodiment, but mean "one or more but not all embodiments", unless otherwise specifically emphasized. The terms "comprising", "including", "having" and their variants all mean "including but not limited to", unless otherwise specifically emphasized.

[0045] Example 1: Referring to Figures 1-9 , a perfusion device, the device includes a base 1, a separation box 2 is fixedly installed on one side of the top of the base 1, a cover plate 3 is clamped on the separation box 2, four electric push rods 4 are symmetrically and fixedly installed on the top of the base 1, and the output shaft of the electric push rod 4 is fixedly connected to the cover plate 3. The telescopic movement of the electric push rod 4 can drive the cover plate 3 to lift and lower, so as to facilitate the opening and closing operation of the separation box 2. A crushing box 5 is fixedly installed through the inner wall of the top of the cover plate 3, a feeding box 6 is fixedly installed on the inner wall of the top of the crushing box 5, and the top of the feeding box 6 extends above the crushing box 5 for feeding embryonic tissue into the device. A feeding pipe 24 is fixedly installed through the inner wall of one side of the feeding box 6 to facilitate feeding embryonic tissue into the feeding box 6.

[0046] Such as Figure 2 , Figure 5 and Figure 7As shown, the device further includes a cutting mechanism, which is installed on the inner wall of the top of the feeding box 6. The bottom of the cutting mechanism extends into the crushing box 5 and is connected to the inner wall of the crushing box 5. The cutting mechanism specifically includes a mounting frame 7 rotatably connected in the crushing box 5. A plurality of material discharging holes 13 are equidistantly arranged on the mounting frame 7. A conical disc 8 is fixedly installed in the mounting frame 7, and a transmission assembly is installed on the top of the conical disc 8. The transmission assembly includes two limiting rods 9 symmetrically and fixedly installed on the top of the conical disc 8. The same limiting plate 10 is slidably connected to the two limiting rods 9. A mounting rod 19 is fixedly installed on the inner wall of the center of the top of the feeding box 6. The bottom end of the mounting rod 19 extends into the crushing box 5 and is fixedly installed with a ball screw 20. A sliding ring 21 that matches the spiral groove on the ball screw 20 is arranged on the ball screw 20. The bottom end of the ball screw 20 penetrates through the limiting plate 10 and is rotatably connected to the top of the conical disc 8. The limiting plate 10 is fixedly connected to the inner wall of the moving frame 11. A plurality of separating cutters 12 are fixedly installed at equal intervals on the bottom of the moving frame 11. The separating cutters 12 correspond to the corresponding material discharging holes 13. When the conical disc 8 receives the power from the grinding mechanism and rotates, it can drive the two limiting rods 9 to perform circular motion, thereby driving the limiting plate 10 to rotate. The rotation of the limiting plate 10 drives the sliding ring 21 to rotate. Under the transmission cooperation with the ball screw 20, it can drive the limiting plate 10 to perform longitudinal reciprocating motion, thereby driving the moving frame 11 and the plurality of separating cutters 12 to perform longitudinal reciprocating motion, so as to cut the embryonic tissue and divide the embryonic tissue into small pieces.

[0047] In addition, as Figure 2 shown, a connecting plate 22 is fixedly installed at the top end of the limiting rod 9. The same separating ring 23 is fixedly installed on the two connecting plates 22. The separating ring 23 is rotatably connected to the inner wall of the feeding box 6. After the embryonic tissue is put into the feeding box 6 through the feeding pipe 24, the rotating separating ring 23 can drive the embryonic tissue to perform centrifugal motion, disperse the embryonic components and scatter them on the conical disc 8. Then the embryonic tissue can slide onto the mounting frame 7 through the conical disc 8 for cutting.

[0048] As Figure 2 and Figure 4As shown, the grinding mechanism is installed in the crushing box 5. The top of the grinding mechanism is connected to the cutting mechanism, and the bottom of the grinding mechanism extends to the outside of the separation box 2 and is connected to the outside of the separation box 2. The grinding mechanism includes a conical grinding disc 14 fixedly installed at the center of the bottom of the conical disc 8. A grinding ring 15 is fixedly installed on the inner wall of the crushing box 5. The bottom of the conical grinding disc 14 extends into the grinding ring 15 and cooperates with the inner wall of the grinding ring 15. A driving motor 25 is fixedly installed at the top of one side of the separation box 2. The output shaft of the driving motor 25 extends into the separation box 2 and is fixedly installed with a driving bevel gear 26. A bevel gear ring 27 is fixedly installed at the bottom of the conical grinding disc 14. The driving bevel gear 26 meshes with the bevel gear ring 27. A separation stirring rod 46 is fixedly installed at the center of the bottom of the conical grinding disc 14. The bottom of the separation stirring rod 46 extends into the separation box 2. When the driving motor 25 is started to drive the driving bevel gear 26 to rotate, under the meshing drive with the bevel gear ring 27, the conical grinding disc 14 can be driven to rotate. After the cut embryonic tissue falls onto the conical grinding disc 14, it moves towards the edge of the conical grinding disc 14 under the action of centrifugal force, and the embryonic tissue is ground and crushed in cooperation with the grinding ring 15, so that the stem cells on the embryonic tissue are completely separated.

[0049] As Figure 2 and Figure 6 shown, in order to prevent the ground embryonic tissue from falling into the separation box 2 and affecting the purity of the stem cells, the grinding mechanism further includes an annular mesh cover 16. A first magnet ring 17 is fixedly installed at the bottom of the grinding ring 15. A second magnet ring 18 is fixedly sleeved on the annular mesh cover 16. The second magnet ring 18 is attracted to the first magnet ring 17. The ground embryonic tissue falls into the annular mesh cover 16. When water flows through the annular mesh cover 16, the separated stem cells are washed into the separation box 2.

[0050] This application can be used in the field of biomedical technology and also in other fields applicable to this application.

[0051] Example 2: Refer to Figure 2 、 Figure 3 、 Figure 8 and Figure 9, improved on the basis of Embodiment 1: A perfusion device for extracting placental stem cells, which is applied to the field of biomedical technology. The filtering mechanism is installed on the other side of the top of the base 1, and one side of the filtering mechanism extends into the separation box 2 and is connected to the grinding mechanism. The top of the grinding mechanism extends into the feeding box 6 and is connected to the inner wall of the other side of the feeding box 6. The filtering mechanism includes a collection box 33 fixedly installed on the other side of the top of the base 1. A primary filter plate 34, a secondary filter plate 35, and a biofilm filter plate 36 are sequentially movably clamped in the collection box 33. The tops of the primary filter plate 34, the secondary filter plate 35, and the biofilm filter plate 36 all extend above the collection box 33. A first suction assembly is installed on the inner wall of one side of the top of the collection box 33. One side of the first suction assembly extends into the separation box 2 and is connected to the inner wall of one side of the separation box 2. The first suction assembly cooperates with the bevel gear ring 27. A second suction assembly is installed through the other side of the top of the collection box 33. The top of the second suction assembly extends into the feeding box 6 and is connected to the inner wall of the other side of the feeding box 6. The bottom of the second suction assembly penetrates through the cover plate 3 and extends into the separation box 2. The bottom of the second suction assembly is connected to the first suction assembly.

[0052] As Figure 2 shown, the first suction assembly includes a first power box 28 fixedly installed on the inner wall of one side of the separation box 2. One side of the first power box 28 extends outside the separation box 2. A transmission rod 29 is rotatably connected through the inner wall of one side of the first power box 28. One end of the transmission rod 29 is fixedly installed with a first water suction blade 41. The other end of the transmission rod 29 extends into the separation box 2 and is fixedly installed with a driven bevel gear 30. The driven bevel gear 30 meshes with the bevel gear ring 27. A suction pipe 44 is fixedly installed on the inner wall of the bottom of the first power box 28. The bottom end of the suction pipe 44 extends into the separation box 2 and is fixedly connected to the inner wall of the bottom of one side of the separation box 2. A liquid outlet pipe 31 is fixedly installed on the inner wall of the other side of the first power box 28. One end of the liquid outlet pipe 31 extends outside the first power box 28 and is fixedly installed with a delivery cover 32. The bottom of the delivery cover 32 extends into the collection box 33 and is fixedly connected to the inner wall of one side of the top of the collection box 33. Under the meshing drive of the driven bevel gear 30 and the bevel gear ring 27, when the conical grinding disc 14 rotates, it can drive the transmission rod 29 to rotate. The transmission rod 29 drives the first water suction blade 41 to rotate to generate suction, sucking the water mixed with stem cells in the separation box 2 into the first power box 28 through the suction pipe 44. Then, the water is transported to the collection box 33 through the liquid outlet pipe 31 and the delivery cover 32. The primary filter plate 34 and the secondary filter plate 35 are used to filter the impurities in the water. After the water flows through the biofilm filter plate 36, the stem cells are filtered. This transmission method can eliminate the need to add an additional power source. Only by starting the drive motor 25 can the entire device be driven to move, which can reduce power consumption and simplify the operation steps, improving the convenience of operation during the entire extraction process.

[0053] As Figure 2 and Figure 3 shown, the second suction component includes a hose 37 that penetrates through the inner wall of the other side of the top of the collection box 33 and is fixedly connected to the inner wall of the other side of the collection box 33. An installation pipe 38 is fixedly installed on the inner wall of the other side of the feeding box 6. One end of the installation pipe 38 extends to the outside of the feeding box 6 and is fixedly installed with a second power box 39. The top end of the hose 37 extends into the second power box 39 and is fixedly connected to the inner wall of the other side of the second power box 39. A blade member is connected through the bottom inner wall of the second power box 39. The bottom of the blade member penetrates through the cover plate 3 and extends into the separation box 2. The blade member is connected to the transmission rod 29. The blade member includes a rotating shaft 40 that penetrates through the bottom inner wall of the second power box 39 and is rotatably connected to the bottom inner wall of the second power box 39. The top end of the rotating shaft 40 is fixedly installed with a second water suction blade 45. The bottom end of the rotating shaft 40 penetrates through the cover plate 3 and extends into the separation box 2. The bottom end of the rotating shaft 40 is fixedly installed with a driving bevel gear 43. A connecting bevel gear 42 located in the separation box 2 is fixedly sleeved on the transmission rod 29. The connecting bevel gear 42 meshes with the driving bevel gear 43. When the transmission rod 29 rotates, it drives the connecting bevel gear 42 to rotate. Under the meshing transmission action with the driving bevel gear 43, it drives the rotating shaft 40 to rotate. The rotating shaft 40 drives the second water suction blade 45 to rotate to generate suction, sucking the water flowing into the collection box 33 into the second power box 39, and then transporting it to the feeding box 6 through the installation pipe 38, enabling the water to circulate and using the water to wash the embryonic tissue.

[0054] When the device is in use, first, the embryonic tissue is put into the feeding box 6 through the blanking pipe 24. The electric push rod 4 is started to drive the cover plate 3 to descend to close the separation box 2. Then, the driving motor 25 is started to drive the conical grinding disc 14 to rotate. The conical grinding disc 14 drives the mounting frame 7 and the conical disc 8 to rotate. The conical disc 8 drives the separation cutter 12 to perform longitudinal reciprocating motion through the transmission component to cut the embryonic tissue. The cut embryonic tissue falls on the conical grinding disc 14 for grinding and crushing, and the separated stem cells fall into the separation box 2. At the same time, the first suction component and the second suction component operate to generate suction to attract the water mixed with stem cells into the collection box 33 for filtration. The water in the collection box 33 is transported to the feeding box 6 through the second suction component for recycling. Through this device, the efficient extraction of placental stem cells can be realized, and the operation is simple, improving the extraction efficiency and purity. After the stem cell extraction is completed, the four electric push rods 4 can be started to push the cover plate 3 upward. Then, it is convenient to clean the separation box 2. At the same time, by pulling down the annular mesh cover 16, the first magnet ring 17 is separated from the second magnet ring 18, and the embryonic tissue falling into the annular mesh cover 16 can be cleaned.

[0055] However, as is well-known to those skilled in the art, the working principles and wiring methods of the electric push rod 4 and the drive motor 25 are common knowledge, and they both belong to conventional means or well-known common sense, so they will not be elaborated here. Those skilled in the art can make arbitrary selections according to their needs or convenience.

[0056] The above is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention can easily think of changes or substitutions, which should all be covered within the protection scope of the present invention; without conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A perfusion device for placental stem cell extraction, comprising a base (1), on one side of the top of the base (1), a separation box (2) is fixedly installed, a cover plate (3) is clamped on the separation box (2), four electric push rods (4) are symmetrically and fixedly installed on the top of the base (1), the output shaft of the electric push rod (4) is fixedly connected with the cover plate (3), a crushing box (5) is fixedly installed through the inner wall of the top of the cover plate (3), a feeding box (6) is fixedly installed on the inner wall of the top of the crushing box (5), the top of the feeding box (6) extends above the crushing box (5), and a feeding pipe (24) is fixedly installed through one side inner wall of the feeding box (6), characterized in that, The perfusion device further includes: a cutting mechanism, which is installed on the inner wall of the top of the feeding box (6), the bottom of the cutting mechanism extends into the crushing box (5) and is connected to the inner wall of the crushing box (5), and the cutting mechanism is used for crushing and cutting embryonic tissues; a grinding mechanism, which is installed in the crushing box (5), the top of the grinding mechanism is connected to the cutting mechanism, and the bottom of the grinding mechanism extends to the outside of the separation box (2) and is connected to the outside of the separation box (2); a filtering mechanism, which is installed on the other side of the top of the base (1), one side of the filtering mechanism extends into the separation box (2) and is connected to the grinding mechanism, and the top of the grinding mechanism extends into the feeding box (6) and is connected to the inner wall of the other side of the feeding box (6).

2. The perfusion device for placental stem cell extraction according to claim 1, characterized in that, The cutting mechanism includes a mounting frame (7) rotatably connected in the crushing box (5). A plurality of material discharging holes (13) are equidistantly formed in the mounting frame (7). A conical disc (8) is fixedly installed in the mounting frame (7). The grinding mechanism is connected to the bottom of the conical disc (8). A transmission assembly is installed on the top of the conical disc (8). A moving frame (11) is connected to the transmission assembly. A plurality of separating cutters (12) are equidistantly and fixedly installed at the bottom of the moving frame (11). The separating cutters (12) correspond to the corresponding material discharging holes (13). The top end of the transmission assembly extends into the feeding box (6) and is connected to the inner wall of the center of the top of the feeding box (6).

3. The perfusion device for placental stem cell extraction according to claim 2, characterized in that, The transmission assembly includes two limiting rods (9) symmetrically and fixedly installed on the top of the conical disc (8). The same limiting plate (10) is slidably connected to the two limiting rods (9). The limiting plate (10) is fixedly connected to the inner wall of the moving frame (11). An installation rod (19) is fixedly installed on the inner wall of the center of the top of the feeding box (6). The bottom end of the installation rod (19) extends into the crushing box (5) and a ball screw (20) is fixedly installed. A sliding ring (21) which is matched with the spiral groove on the ball screw (20) is arranged on the ball screw (20). The bottom end of the ball screw (20) penetrates through the limiting plate (10) and is rotatably connected to the top of the conical disc (8).

4. The perfusion device for placental stem cell extraction according to claim 3, characterized in that, The top end of the limiting rod (9) is fixedly installed with a connecting plate (22), and the same separating ring (23) is fixedly installed on the two connecting plates (22). The separating ring (23) is rotatably connected to the inner wall of the feeding box (6).

5. The perfusion device for placental stem cell extraction according to claim 1, characterized in that, The grinding mechanism includes a conical grinding disc (14) fixedly installed at the central position of the bottom of the conical disc (8), and a grinding ring (15) is fixedly installed on the inner wall of the crushing box (5). The bottom of the conical grinding disc (14) extends into the grinding ring (15) and is matched with the inner wall of the grinding ring (15). A driving motor (25) is fixedly installed at the top of one side of the separation box (2). The output shaft of the driving motor (25) extends into the separation box (2) and is fixedly installed with a driving bevel gear (26). And a bevel gear ring (27) is fixedly installed at the bottom of the conical grinding disc (14). The driving bevel gear (26) meshes with the bevel gear ring (27). A separation stirring rod (46) is fixedly installed at the central position of the bottom of the conical grinding disc (14). The bottom of the separation stirring rod (46) extends into the separation box (2).

6. The perfusion device for placental stem cell extraction according to claim 5, characterized in that, The grinding mechanism further includes an annular mesh cover (16). A first magnet ring (17) is fixedly installed at the bottom of the grinding ring (15). And a second magnet ring (18) is fixedly sleeved on the annular mesh cover (16). The second magnet ring (18) is attracted to the first magnet ring (17).

7. The perfusion device for placental stem cell extraction according to claim 1, wherein The filtering mechanism includes a collection box (33) fixedly installed on the other side of the top of the base (1). A primary filter plate (34), a secondary filter plate (35), and a biofilm filter plate (36) are sequentially movably clamped in the collection box (33). The tops of the primary filter plate (34), the secondary filter plate (35), and the biofilm filter plate (36) all extend above the collection box (33). A first attracting component is installed on the inner wall of one side of the top of the collection box (33). One side of the first attracting component extends into the separation box (2) and is connected to the inner wall of one side of the separation box (2). The first attracting component is matched with the bevel gear ring (27). A second attracting component is installed through the other side of the top of the collection box (33). The top of the second attracting component extends into the feeding box (6) and is connected to the inner wall of the other side of the feeding box (6). The bottom of the second attracting component penetrates through the cover plate (3) and extends into the separation box (2). The bottom of the second attracting component is connected to the first attracting component.

8. The perfusion device for placental stem cell extraction according to claim 7, characterized in that, The first suction component includes a first power box (28) fixedly installed on the inner wall of one side of the separation box (2). One side of the first power box (28) extends to the outside of the separation box (2). A transmission rod (29) is rotatably connected through the inner wall of one side of the first power box (28). One end of the transmission rod (29) is fixedly installed with a first water suction blade (41). One end of the transmission rod (29) extends into the separation box (2) and is fixedly installed with a driven bevel gear (30). The driven bevel gear (30) meshes with the bevel gear ring (27). A suction pipe (44) is fixedly installed on the bottom inner wall of the first power box (28). The bottom end of the suction pipe (44) extends into the separation box (2) and is fixedly connected to the bottom inner wall of one side of the separation box (2). A liquid outlet pipe (31) is fixedly installed on the inner wall of the other side of the first power box (28). One end of the liquid outlet pipe (31) extends to the outside of the first power box (28) and is fixedly installed with a delivery cover (32). The bottom of the delivery cover (32) extends into the collection box (33) and is fixedly connected to the top inner wall of one side of the collection box (33).

9. The perfusion device for placental stem cell extraction according to claim 7 or 8, characterized in that, The second suction component includes a hose (37) that penetrates through the top inner wall of the other side of the collection box (33) and is fixedly connected to the inner wall of the other side of the collection box (33). An installation pipe (38) is fixedly installed on the inner wall of the other side of the feeding box (6). One end of the installation pipe (38) extends to the outside of the feeding box (6) and is fixedly installed with a second power box (39). The top end of the hose (37) extends into the second power box (39) and is fixedly connected to the inner wall of the other side of the second power box (39). A blade member is connected through the bottom inner wall of the second power box (39). The bottom of the blade member penetrates through the cover plate (3) and extends into the separation box (2). The blade member is connected to the transmission rod (29).

10. The perfusion device for placental stem cell extraction according to claim 9, characterized in that, The blade member includes a rotating shaft (40) that penetrates through the bottom inner wall of the second power box (39) and is rotatably connected to the bottom inner wall of the second power box (39). The top end of the rotating shaft (40) is fixedly installed with a second water suction blade (45). The bottom end of the rotating shaft (40) penetrates through the cover plate (3) and extends into the separation box (2). The bottom end of the rotating shaft (40) is fixedly installed with a driving bevel gear (43). A connecting bevel gear (42) located in the separation box (2) is fixedly sleeved on the transmission rod (29). The connecting bevel gear (42) meshes with the driving bevel gear (43).

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