Potato starch processing waste residue fermentation device and method

Abstract

The invention discloses a potato starch processing waste residue fermentation device and method. The device comprises a tank body with a sterile gas filtering discharge port, an automatic inoculation box is arranged outside the tank body, and a stirring device and an automatic gas exchange device are arranged inside the tank body; the automatic inoculation box is mounted above the side of the tank body, a bacterial liquid bottle and an alcohol lamp are arranged in the automatic inoculation box, the bacterial liquid bottle is connected with an inoculation opening communicated with the interior of the tank body through a closed pipeline and feeds bacterial liquid into the fermentation system, and the alcohol lamp is arranged below the inoculation opening and heats the inoculation opening; the automatic gas exchange device comprises a pressure sensor and a plurality of air distributors, and sterile air equipment outside the tank body is communicated with each air distributor through a sterile air inlet and inputs sterile air into the fermentation system; the pressure sensor is mounted at the sterile gas filtering and discharging port, provides a real-time air pressure signal for the PLC control system and controls the gas discharging of the sterile gas filtering and discharging port, so that the problems that the existing fermentation lacks a proper device and the fermentation efficiency is low are finally solved.

Description

Technical Field

[0001] The present invention belongs to the technical field of agricultural waste treatment, and particularly relates to a fermentation device and method for potato starch processing waste residue. Background Technique

[0002] Potato starch processing waste residue is the main by-product in the process of potato starch production, and the amount of waste residue generated is about 7 times that of refined starch. Along with starch production, the environmental pollution problem caused by waste residue has become increasingly prominent, becoming a limiting factor for the development of starch processing enterprises. It is known that the waste residue contains a large amount of organic substances such as starch, cellulose, pectin, and protein, and has great utilization value. At present, the most common utilization method is to ferment it into biological organic fertilizer.

[0003] However, the existing fermentation devices can only ferment a single raw material, and the fermentation efficiency is not high. Secondly, during the fermentation process, the changes in pH, temperature, and pressure in the fermentation tank cannot be detected in real time, and cannot be adjusted in time. In addition, in the current production method, manual stirring is mostly used, the sealing is not tight, the fermentation is easily contaminated, the product is difficult to control, and the discharging intensity is large, which is not suitable for the needs of modern production. Summary of the Invention

[0004] In order to overcome the above-mentioned disadvantages of the prior art, the purpose of the present invention is to provide a fermentation device and method for potato starch processing waste residue to solve the problems of lack of a suitable device for existing fermentation and low fermentation efficiency.

[0005] In order to achieve the above purpose, the technical solution adopted by the present invention is:

[0006] A fermentation device for potato starch processing waste residue includes a tank body with a sterile gas filtration and discharge port. An automatic inoculation box is arranged outside the tank body, and a stirring device and a gas automatic exchange device are arranged inside.

[0007] The automatic inoculation box is installed above the side of the tank body. A bacterium liquid bottle and an alcohol lamp are arranged inside. The bacterium liquid bottle is connected to an inoculation port communicating with the inside of the tank body through a sealed pipeline to send bacterium liquid into the fermentation system. The alcohol lamp is arranged below the inoculation port to heat the inoculation port.

[0008] The gas automatic exchange device includes a pressure sensor and a plurality of air distributors. The sterile air equipment outside the tank body is communicated with each air distributor through a sterile air inlet to input sterile air into the fermentation system. The pressure sensor is installed at the sterile gas filtration and discharge port to provide a real-time air pressure signal to the PLC control system. The PLC control system controls the gas discharge of the sterile gas filtration and discharge port according to the real-time air pressure signal.

[0009] In one embodiment, the stirring device includes a stirring shaft vertically installed in the tank body, and a propeller stirring paddle is vertically installed on the stirring shaft. The propeller stirring paddle includes a forward-rotating stirring paddle and a reverse-rotating stirring paddle, and the forward-rotating stirring paddle with blades and the reverse-rotating stirring paddle with blades are staggered on the stirring shaft.

[0010] In one embodiment, the propeller stirring paddle is a three-blade propeller stirring paddle, and the surfaces and fronts of its blades are both wavy.

[0011] In one embodiment, the root of the propeller stirring paddle blade is connected to a stirring shaft link ring, and a gear sleeve is arranged on the inner wall of the stirring shaft link ring. The propeller stirring paddle is rotationally connected to the stirring shaft by using the gear sleeve and meshes with the outer tooth grooves of the stirring shaft.

[0012] In one embodiment, the forward rotation and reverse rotation of the forward-rotating stirring paddle with blades and the reverse-rotating stirring paddle with blades on the stirring shaft are realized by means of reverse gear transmission, reverse differential transmission, double-shaft nested reverse drive or independent drive.

[0013] In one embodiment, the reverse gear transmission is realized by a planetary gear mechanism or an idler gear set; in the planetary gear mechanism, the sun gear is fixedly installed on the stirring shaft, the reverse-rotating stirring paddle with blades is fixedly installed on the outer gear ring, and the forward-rotating stirring paddle with blades is directly fixedly connected to the stirring shaft; in the idler gear set, a driving gear is driven by the stirring shaft, an idler is added in the middle, and then the driven gear of the reverse-rotating stirring paddle with blades is meshed, and the forward-rotating stirring paddle with blades is directly fixedly connected to the stirring shaft.

[0014] In one embodiment, a temperature sensor and a pH meter are arranged in the tank body. The PLC control system adjusts the temperature to 28 - 30 °C through a jacketed heat exchanger arranged on the inner wall of the tank body according to the real-time temperature, pH and pressure, adjusts the pH to 6.5 - 7.5 through an acid-base tank, inputs sterile air into the tank through a sterile air inlet and an air distributor when the pressure in the tank is lower than the lower limit of the set pressure threshold range, and discharges the gas generated by fermentation through a sterile gas filtration discharge port when the pressure in the tank is higher than the upper limit of the set pressure threshold range.

[0015] In one embodiment, the outlet of the air distributor is horizontally arranged, or inclined downward, or vertically downward; a small fan blade is installed at the air outlet of the air distributor.

[0016] The present invention also provides a fermentation method based on the potato starch processing waste residue fermentation device. Potato starch processing waste residue with a water content of more than 80% is put into the tank body, and the fermentation conditions are set as follows: temperature 28 - 30 °C, pH 6.5 - 7.5, pressure 0.05 - 0.08 Mpa. The fermentation tank is operated for 12 hours according to the above fermentation conditions. After the fermentation conditions in the material are stable, the bacterial liquid is added.

[0017] In one embodiment, the bacterial liquid is the liquid obtained by inoculating a monoclonal colony of the strain in an LB liquid medium and culturing it on a shaker at 120 revolutions per minute and a culture temperature of 30 °C for 3 days. The strain of the effective viable bacteria is Pseudomonas chlororaphis, and the taxonomic name is Pseudomonas chlororaphis. The Pseudomonas chlororaphis has been deposited in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms, with the address at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit date is December 20, 2011, and the deposit number is CGMCC No. 5628.

[0018] In one embodiment, the inoculation amount used is 0.8% of the volume of the material, and the concentration of the bacterial liquid is OD 600 = 1.0.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0020] 1. The present invention sets an automatic inoculation box on the tank body and sends the bacterial liquid into the tank through a closed pipeline to achieve aseptic operation and avoid contamination by miscellaneous bacteria and phages.

[0021] 2. The present invention sets a gas automatic exchange device, which can achieve aseptic gas exchange during the fermentation process, maintain the stable air pressure required for fermentation in the tank, enable the materials inside the tank body to fully ferment with the strain, and reduce the labor input.

[0022] 3. The present invention improves the stirring effect by simultaneously stirring forward and backward.

[0023] 4. The present invention uses a PLC control system to automatically regulate the temperature, pressure, and pH, achieving long-term continuous operation.

[0024] 5. The present invention adds a specific potassium-dissolving, phosphorus-solubilizing, and nitrogen-fixing bacterial liquid during the fermentation process to improve the fermentation effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 is a schematic structural diagram of the present invention.

[0026] Figure 2 is a schematic structural diagram of the stirring device of the present invention.

[0027] Figure 3It is a schematic diagram of the installation structure of the propeller agitator of the present invention.

[0028] Figure 4 It is a schematic diagram of the blade shape structure of the propeller agitator of the present invention. Detailed implementation manners

[0029] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. The following specific embodiments illustrate the implementation manners of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of them. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

[0030] As Figure 1 shown, the present invention is a fermentation device for potato starch processing waste residue, which includes a tank body 1. For the convenience of operation, the tank body 1 is preferably a cylindrical structure, and a stirring device and the like can be arranged inside to stir the materials during the fermentation process. In its basic structure, the feed inlet 11 is located at the top of the tank body 1 and has a sight glass function. The discharge outlet 12 is located obliquely downward at the bottom of the tank body 1, and a lighting device 13 is arranged at the top end inside the tank. The feed inlet 11 has a sight glass function and is used in cooperation with the lighting device 13 to facilitate the observation of the fermentation condition.

[0031] The key of the present invention lies in that, on the one hand, considering that the prior art ignores the promoting effect of strains in fermentation, an automatic inoculation box 9 is arranged outside the tank body 1. On the other hand, considering that the prior art ignores the adverse impact of fermentation gas on the long-term operation of the fermentation system, a gas automatic exchange device is arranged inside the tank body 1.

[0032] Among them, the automatic inoculation box 9 is a closable box, which is installed at a position above the outer wall of the tank body 1. A bacterium liquid bottle 9-4 and an alcohol lamp 9-2 are arranged inside. An inoculation port 9-3 is opened on the side wall of the tank body 1. The inoculation port 9-3 is located in the automatic inoculation box 9 and is connected to the bacterium liquid bottle 9-4 through a sealed pipeline. A small pressure pump 9-5 can be arranged, so that the bacterium liquid can be directly sent into the fermentation system from the tank to avoid contamination. The alcohol lamp 9-2 is arranged below the inoculation port 9-3 to heat the inoculation port to create a sterile environment to ensure aseptic operation and make the fermentation pollution-free. For example, the small pressure pump 9-5 can be controlled by a pressure pump switch 9-1, and the alcohol lamp 9-2 can be replaced by other heating devices such as an infrared inoculation loop sterilizer.

[0033] The gas automatic exchange device includes a pressure sensor 7 and several air distributors 6. A sterile air inlet 5 and a sterile gas filtration and discharge port 8 are provided on the tank body 1. The sterile air inlet 5 is communicated with each air distributor 6. The pressure sensor 7 is preferably installed at the sterile gas filtration and discharge port 8 and is connected to the PLC control system 14. Thus, the sterile air equipment outside the tank body 1 can input sterile air into the fermentation system through the sterile air inlet 5 and each air distributor 6 to ensure no contamination by miscellaneous bacteria during the fermentation process. The PLC control system 14 can control the gas discharge of the sterile gas filtration and discharge port 8 according to the real-time air pressure signal provided by the pressure sensor 7. Specifically, it can be achieved by setting an electric valve and an air extractor at the sterile gas filtration and discharge port 8, etc.

[0034] According to the above structure, the present invention can manually or automatically add bacterial liquid into the fermentation system in the tank as needed, realize the co-fermentation of waste residue and strains, improve the fermentation effect, and the addition method is convenient. And it can automatically control the exhaust or the introduction of sterile gas according to the real-time air pressure signal, maintain a good fermentation environment, and ensure the long-term stable operation of the system.

[0035] In an embodiment of the present invention, the inner cavity bottom of the tank body 1 is arranged in a downward concave arc-shaped structure to well collect the fermentation products. The fermentation products in the tank body 1 are discharged along the discharge port 12, which can not only ensure the rotation of the stirring shaft 2, but also does not affect the discharging of the discharge port 12, and the arc-shaped tank bottom can also reduce the accumulation of materials.

[0036] In an embodiment of the present invention, referring to Figure 2 As shown, the stirring device includes a stirring shaft 2 vertically installed in the tank body 1. The stirring shaft 2 is connected to the output of the motor 3 outside the tank and can rotate under the driving action of the motor 3. A propeller stirring paddle 4 is vertically installed on the stirring shaft 2. The propeller stirring paddle 4 rotates synchronously with the stirring shaft 2 to stir the fermentation system, stir the materials in the inner and outer layers evenly, keep the temperature of the materials in the inner and outer layers roughly the same, avoid uneven fermentation of the fermented product, provide a suitable growth environment for the strains, and thus improve the fermentation efficiency. This device can meet the preparation of large-scale fermentation products. Different from the existing stirring methods, in this embodiment, the propeller stirring paddle 4 includes a forward-rotating stirring paddle 15 and a reverse-rotating stirring paddle 16, which can rotate and stir forward and backward respectively. For example, the blade forward-rotating stirring paddle 15 and the blade reverse-rotating stirring paddle 16 are staggered on the stirring shaft 2.

[0037] Through the above positive and reverse rotation stirring, the fermentation materials can be stirred more fully, ensuring the full mixing of the materials, making the temperatures of the components of the mixed materials roughly the same, reducing the influence of temperature on fermentation, providing a suitable environment for the fermentation of microorganisms, and increasing the dissolved oxygen required for fermentation. The temperatures of the inner and outer layers of the mixed materials are quite the same, the fermentation is uniform, and the fermentation efficiency is increased.

[0038] In an embodiment of the present invention, referring to Figure 3 As shown, a specific installation structure of the propeller agitator 4, its blade root is connected to the stirring shaft link ring 19, and the propeller agitators 4 of the same layer are connected to the outer wall of the same stirring shaft link ring 19. A toothed sleeve 18 is arranged on the inner wall of the stirring shaft link ring 19, and this toothed sleeve 18 can be rotationally connected to the stirring shaft 2 and rotate synchronously under the drive of the stirring shaft 2. Specifically, a toothed groove can be arranged on the outside of the stirring shaft 2 and meshed with the toothed sleeve 18 to achieve transmission.

[0039] In an embodiment of the present invention, referring to Figure 4 As shown, the propeller agitator 4 is a three-blade propeller agitator, and both the surface and the front end of its blades are wavy. This shape structure can increase the contact area with the fermentation broth, enhance the fluidity of the mixed materials, and facilitate the mixing of the innermost layer of materials and the outermost layer of materials, enabling the heat exchange between the inner and outer layer of materials, ultimately making the materials ferment fully, improving the stirring effect and fermentation efficiency, and solving the problems of insufficient stirring of materials and unstable products during the fermentation process.

[0040] In an embodiment of the present invention, in order to achieve connecting the blade forward rotation agitator 15 and the blade reverse rotation agitator 16 on the same stirring shaft 2 at the same time, it can be achieved by means such as reverse transmission of gears, reverse transmission of a differential, reverse drive of a double-axis nesting, or independent drive. The structure of the planetary gear mechanism is compact and the torque transmission efficiency is high; the structure of the idler gear group is simple and the cost is low. The present invention preferably uses these two methods to achieve.

[0041] Among them, when using the planetary gear mechanism, the sun gear is fixedly installed on the stirring shaft 2, the blade reverse rotation agitator 16 is fixedly installed on the outer gear ring, and the reverse rotation is achieved through the reverse meshing of the planetary gears. The blade forward rotation agitator 15 is directly fixedly connected to the stirring shaft 2 and rotates forward with the shaft.

[0042] When using the idler gear, a driving gear is driven by the stirring shaft, an idler is added in the middle, and then meshed with the driven gear of the blade reverse rotation agitator 16 to achieve reverse rotation. The blade forward rotation agitator 15 is directly fixedly connected to the stirring shaft 2 and rotates forward with the shaft.

[0043] In an embodiment of the present invention, a jacketed heat exchanger 10 is provided on the inner wall of the tank body 1, which can regulate the temperature inside the tank by introducing different heat exchange media, that is, increase or decrease the temperature, so as to keep the fermentation system at a constant temperature. At the same time, a temperature sensor and a pH meter are provided in the tank in this application, so that the PLC control system 14 can adjust the temperature to the set range through the jacketed heat exchanger 10 provided on the inner wall of the tank body 1 according to the real-time temperature, pH and pressure, adjust the pH to the set range through an external acid-base tank, and when the pressure in the tank is lower than the lower limit of the set pressure threshold range, input sterile air into the tank through the sterile air inlet 5 and the air distributor 6, and when the pressure in the tank is higher than the upper limit of the set pressure threshold range, discharge the gas generated by fermentation through the sterile gas filtration discharge port 8, so as to adjust the pressure in the tank to the set range.

[0044] In an embodiment of the present invention, the outlet of the air distributor 6 is horizontally arranged, or inclined downward, or vertically downward to prevent the viscous fermentation material from blocking the outlet. Further, a small fan blade can be installed at its air outlet. When ventilating, the fan blade can rotate as the air flow enters, displacing the surrounding fermentation material, so as to achieve full mixing of the sterile air and the fermentation material. This outlet can also be provided with an anti-blocking baffle, which is designed to open only in the air flow direction, is opened under the air flow pressure, and automatically closes in the absence of air flow to prevent the material from blocking the outlet. During the fermentation process, the pressure sensor 7 continuously detects the air pressure inside the tank body 1 to ensure that the sterile gas filtration discharge port 8 timely discharges the gas generated by fermentation, maintains the stable air pressure required for fermentation inside the tank body 1, and reduces the influence on the fermentation of internal strains.

[0045] For the fermentation method using the fermentation device of the present invention, devices such as a mechanical pump can be used to input potato starch processing waste residue with a water content of more than 80% into the tank body 1 through the feed port 11, and the fermentation conditions are set as follows: the optimum temperature is 28-30°C, the optimum pH is 6.5-7.5, and the optimum pressure is 0.05-0.08 Mpa. Operate the fermentation tank for 12 hours according to the above fermentation conditions. After the fermentation conditions in the material are stable, start the small pressure pump 9-5 and the alcohol lamp 9-2, and add the fermentation bacterial liquid.

[0046] During the fermentation process, the above fermentation conditions can be strictly controlled through the PLC control system 14. Specifically:

[0047] The fermentation tank ferments normally within the range of 28-30°C. When it exceeds this range, for example, when it is lower than 28°C, control the jacketed heat exchanger 10 to automatically turn on, and heat the fermentation material by injecting a non-phase-change heating agent, etc.; when the fermentation temperature is higher than 30°C, control the jacketed heat exchanger 10 to automatically turn on, and cool the fermentation material by injecting cold water, etc., so that the fermentation temperature of the material is always controlled within the optimum temperature range.

[0048] Normal fermentation occurs within the pH range of 6.5 - 7.5. When outside this range, for example, when the pH is lower than 6.5, the PLC control system 14 injects NaOH into the tank body 1 through the acid-base tank it controls to increase the pH. When the pH is higher than 7.5, the PLC control system 14 injects HCl into the tank body 1 through the acid-base tank it controls to lower the pH, so that the pH of the material fermentation is always controlled within the optimal range.

[0049] Normal fermentation occurs within the range of 0.05 - 0.08 Mpa. When outside this range, for example, when it is lower than 0.05 Mpa, the sterile air equipment outside the tank body (1) inputs sterile air into the fermentation system through the sterile air inlet 5 and each air distributor (6) so that the fermentation pressure in the tank is not lower than 0.05 Mpa. When the fermentation pressure is higher than 0.08 Mpa, the gas generated by fermentation is controlled to be discharged through the sterile gas filtration and discharge port 8, so that the temperature of the material fermentation is always controlled within the optimal temperature threshold.

[0050] According to the above structure and control strategy, the present invention can automatically monitor and record the changes in the temperature and pH inside the tank body 1. The PLC control system automatically adjusts the temperature to keep the internal temperature of the fermentation constant. The operation is simple and requires little manual intervention.

[0051] In the embodiment of the present invention, the tank body 1 operates at full load, that is, the potato starch processing waste residue added to it fills its interior. For example, when its volume is 500 liters, 500 liters of material can be added. Exemplarily, the material of the tank body 1 can be stainless steel, and its inner surface is smooth and not easy to adhere to the wall.

[0052] In an embodiment of the present invention, a special bacterial liquid is used. It is the liquid obtained by inoculating a single clone colony of the strain in an LB liquid medium and placing it on a shaker at 120 revolutions per minute and a culture temperature of 30 degrees Celsius for 3 days. The strain of the effective viable bacteria is Pseudomonas chlororaphis, and the preservation center is CGMCC, with the number 5628. The prior art shows that this strain is a potassium-solubilizing bacterium, and the potassium-solubilizing amount in the culture solution is about 3.1 μg / mL. For example, in the patent CN105254356A, it is clearly defined as a potassium-solubilizing bacterium and used in biological organic fertilizers.

[0053] The applicant determined through further experiments and found that it also has the function of decomposing inorganic phosphorus. The experimental process for quantitatively measuring the strain ability is as follows:

[0054] Quantitative determination of phosphorus solubilization: In a 100 mL Erlenmeyer flask, 50 mL of sterilized inorganic phosphorus liquid medium was filled. 2.5 mL of the bacterial suspension to be tested was inoculated into the inorganic phosphorus liquid medium respectively, and a control group was set up with 3 replicates. It was cultured at 30 °C and 120 r / min for 5 days. The culture solution was centrifuged at 4 °C and 10,000 r / min for 10 min, and the supernatant was taken to determine the available phosphorus content by the molybdenum antimony anti-colorimetric method.

[0055] The nitrogen fixation ability of the strain was expressed by nitrogenase activity. Determination of nitrogenase activity: The nitrogenase activity of the strain was determined by the acetylene (C2H2) reduction method. The strain was inoculated into 5 mL of semi-solid NFM medium (serum bottle, specification 15 mL) with an inoculation loop respectively, with 3 replicates for each strain. The medium without inoculation was used as the control, sealed with a cotton plug, and cultured at 28 °C for 48 h. Then the cotton plug was replaced with a rubber plug, 1 mL of gas was drawn out with a sterile syringe, and then 1 mL of C2H2 gas was injected, and it was cultured in an incubator at 28 °C for 48 h. 50 μL of the mixed gas was drawn from the serum bottle with a 100 μL microsyringe and injected into the gas injection column of the gas chromatograph, and the C2H2 peak time and peak area were recorded and observed, and the C2H2 content was calculated. The results are shown in Table 1.

[0056] Quantitative determination of potassium solubilization: The single bacterial population obtained by separation and purification was inoculated in the modified liquid medium, with potassium feldspar powder as the only potassium source, and cultured on a shaker at 30 °C and 120 r / min for 10 d. 10.0 mL of the culture solution was centrifuged at 8,000 r / min for 8 min, and the supernatant was taken. The potassium solubilization amount in the supernatant was determined by atomic absorption spectrophotometry. Compared with the blank control group without inoculating potassium-solubilizing bacteria, it was repeated 3 times, and the results of each determination were recorded. The results are shown in Table 1.

[0057] Table 1

[0058]

[0059] The results showed that the phosphorus solubilization ability of the strain in the culture solution was 365.9 μg / mL, and the nitrogen fixation ability was 483.4 (nmolC2H4h -1 ·mL -1 ), it could utilize the residual organic matter in the potato starch processing waste residue to produce a variety of secondary metabolites, making its fermentation products have rich growth-promoting active substances. And this strain itself was a plant growth-promoting bacterium. In addition, it could fully adapt to the fermentation environment, increase the content of viable bacteria of beneficial microorganisms in the fermentation products, and achieve the effect of effectively promoting plant growth.

[0060] In one embodiment of the present invention, when co-fermenting with potato starch processing waste residue, the inoculation amount used in the fermentation system was 0.8% of the volume of the material, and the concentration of the bacterial liquid was OD 600= 1.0. The final co-fermentation product of potato starch processing waste residue and microorganisms is a highly efficient biological organic fertilizer.

[0061] The above embodiments are only exemplary embodiments of the present application and are not used to limit the present application. The protection scope of the present application is defined by the claims. Those skilled in the art can make various modifications or equivalent replacements within the essence and protection scope of the present application, and such modifications or equivalent replacements should also be regarded as falling within the protection scope of the present application.

Claims

1. A fermentation device for potato starch processing waste residue, characterized in that, It includes a tank body (1) with a sterile gas filtration and discharge port (8). An automatic inoculation box (9) is arranged outside the tank body (1), and a stirring device and a gas automatic exchange device are arranged inside. The automatic inoculation box (9) is installed above the side of the tank body (1). A bacterial liquid bottle (9-4) and an alcohol lamp (9-2) are arranged inside it. The bacterial liquid bottle (9-4) is connected to an inoculation port (9-3) communicating with the inside of the tank body (1) through a sealed pipeline to send bacterial liquid into the fermentation system. The alcohol lamp (9-2) is arranged below the inoculation port (9-3) to heat the inoculation port (9-3). The gas automatic exchange device includes a pressure sensor (7) and a plurality of air distributors (6). The sterile air equipment outside the tank body (1) is communicated with each air distributor (6) through a sterile air inlet (5) to input sterile air into the fermentation system. The pressure sensor (7) is installed at the sterile gas filtration and discharge port (8) to provide a real-time air pressure signal to the PLC control system (14). The PLC control system (14) controls the gas discharge of the sterile gas filtration and discharge port (8) according to the real-time air pressure signal.

2. The potato starch processing waste residue fermentation device according to claim 1, wherein, The stirring device includes a stirring shaft (2) vertically installed inside the tank body (1). A propeller stirring paddle (4) is vertically installed on the stirring shaft (2). The propeller stirring paddle (4) includes a forward-rotating stirring paddle (15) and a reverse-rotating stirring paddle (16). The forward-rotating stirring paddle (15) with blades and the reverse-rotating stirring paddle (16) with blades are staggeredly distributed on the stirring shaft (2).

3. The potato starch processing waste residue fermentation device according to claim 2, wherein, The propeller stirring paddle (4) is a three-blade propeller stirring paddle, and the surfaces and fronts of its blades are both wavy.

4. The potato starch processing waste residue fermentation device according to claim 2 or 3, characterized in that, The forward rotation and reverse rotation of the forward-rotating stirring paddle (15) with blades and the reverse-rotating stirring paddle (16) with blades on the stirring shaft (2) are realized by means of reverse gear transmission, reverse differential transmission, double-shaft nested reverse drive or independent drive.

5. The potato starch processing waste residue fermentation device according to claim 4, characterized in that, The reverse gear transmission is realized by a planetary gear mechanism or an idler gear set. In the planetary gear mechanism, the sun gear is fixedly installed on the stirring shaft (2), the reverse-rotating stirring paddle (16) with blades is fixedly installed on the outer gear ring, and the forward-rotating stirring paddle (15) with blades is directly fixedly connected to the stirring shaft (2). In the idler gear set, the stirring shaft drives a driving gear, an idler is added in the middle, and then the driven gear of the reverse-rotating stirring paddle (16) with blades is engaged, and the forward-rotating stirring paddle (15) with blades is directly fixedly connected to the stirring shaft (2).

6. The potato starch processing waste residue fermentation device according to claim 1, characterized in that A temperature sensor and a pH meter are arranged inside the tank body (1). The PLC control system (14) adjusts the temperature to 28-30 °C through a jacketed heat exchanger (10) arranged on the inner wall of the tank body (1) according to the real-time temperature, pH and pressure, adjusts the pH to 6.5-7.5 through an acid-base tank, and inputs sterile air into the tank through a sterile air inlet (5) and an air distributor (6) when the pressure in the tank is lower than the lower limit of the set pressure threshold range. When the pressure in the tank is higher than the upper limit of the set pressure threshold range, the gas generated by fermentation is discharged through a sterile gas filtration and discharge port (8).

7. The fermentation device for potato starch processing waste residue according to claim 1, characterized in that, The outlet of the air distributor (6) is arranged horizontally, or inclined downward, or vertically downward; at the air outlet of the air distributor (6), small fan blades are installed.

8. The fermentation method of the fermentation device for potato starch processing waste residue according to claim 1, characterized in that, Potato starch processing waste residue with a water content of more than 80% is put into the tank body (1). The fermentation conditions are set as follows: temperature 28-30 °C, pH 6.5-7.5, pressure 0.05-0.08 Mpa. The fermentation tank is operated for 12 hours according to the fermentation conditions. After the fermentation conditions in the material are stable, a bacterial solution is added. The strain of the effective viable bacteria in the bacterial solution is Pseudomonas chlororaphis, and the preservation center is CGMCC, with the number 5628.

9. The fermentation method according to claim 8, wherein The bacterial solution is the liquid obtained by inoculating a single clone colony of the strain in an LB liquid medium and culturing it on a shaker at 120 revolutions per minute and a culture temperature of 30 °C for 3 days.

10. The fermentation method according to claim 8, characterized in that, The inoculation amount used is 0.8% of the material volume, and the concentration of the bacterial solution is OD 600 = 1.0.

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