Ceramide composition capable of promoting cell repair as well as preparation method and application of ceramide composition

Abstract

The invention discloses a ceramide composition capable of promoting cell repair and a preparation method and application thereof, and belongs to the technical field of foods.The ceramide composition is prepared from, by weight, 0.5-2 parts of rice bran oil powder, 0.5-2 parts of salvia officinalis extract and 5-32 parts of bioactive peptide. The bioactive peptides comprise fish collagen peptide, cubilose peptide and sea cucumber peptide, and the weight ratio of the fish collagen peptide to the cubilose peptide to the sea cucumber peptide is (30-55): 1: (1-10). Through the synergistic effect of the rice bran oil powder, the salvia officinalis extract and the bioactive peptide, cell repair is promoted jointly, and efficient repair of a skin barrier is achieved.

Description

Technical Field

[0001] The present invention belongs to the technical field of food, and specifically relates to a ceramide composition capable of promoting cell repair, a preparation method thereof, and an application thereof. Background Art

[0002] As the largest organ of the human body, the skin has barrier and various immune functions, and the skin barrier plays an important role in preventing water loss, resisting external stimuli, and maintaining the skin from infection. Narrowly speaking, the skin barrier refers to a physiological structure on the surface layer of the skin, mainly composed of keratinocytes, intercellular lipids, desmosomes, and tight junctions. The complete skin barrier structure is the basis of the body's physiological response. When the skin barrier is damaged, the protective function of the skin weakens, making it vulnerable to external factors, which can induce and exacerbate a series of skin problems.

[0003] As a key active ingredient in intercellular lipids, ceramide plays an irreplaceable role in promoting cell repair. It not only participates in the construction of intercellular lipids and maintains the tightness of the skin barrier, but also regulates the processes of proliferation, differentiation, and apoptosis of keratinocytes. When the skin barrier is damaged, ceramide can promote the repair of damaged cells, accelerate cell metabolism, and help cells restore normal physiological functions, thereby repairing and strengthening the skin barrier at the cellular level.

[0004] Patent CN120000561A discloses a barrier repair composition containing Pinus densiflora leaf extract and olive ceramide NP, and patent CN117860631A discloses a skin care composition containing ingredients such as avocado resin, ceramide NP, hyaluronic acid, and bisabolol. However, both of these two technologies are only used for external application. However, the percutaneous absorption rate of topical ceramide is poor, and the dense structure of the skin stratum corneum limits the penetration of ceramide. A single ceramide subtype is difficult to achieve a repair effect. Moreover, topical ceramide is mostly for short-term repair, lacking a mechanism for continuous release or stimulating the synthesis of endogenous ceramide, and it is difficult to maintain the homeostasis of the skin barrier structure and function.

[0005] Most of the skin barrier repair solutions have serious ingredient homogenization and lack proven comprehensive synergistic effects. For example, the collagen tripeptide that promotes the proliferation of skin keratinocytes disclosed in patent CN117659118A. This invention plays a certain role in promoting skin barrier repair by preparing collagen peptides with special peptide segments, but lacks a multi-target synergistic effect design. Summary of the Invention

[0006] To solve the above technical problems, the present invention provides a ceramide composition capable of promoting cell repair, a preparation method thereof, and an application thereof. Through the synergistic effect between raw materials, the ceramide composition jointly promotes cell repair and achieves effective repair of the skin barrier.

[0007] To achieve the above object, the present invention adopts the following technical solutions: In a first aspect, the present invention provides a ceramide composition capable of promoting cell repair, which is composed of the following raw materials in parts by weight: 0.5-2 parts of rice bran oil powder, 0.5-2 parts of sage extract, and 5-32 parts of bioactive peptide.

[0008] In the present invention, the rice bran oil powder contains oryzanol, phytosterols, and ceramide analogs, which can strengthen the cell membrane structure, resist free radical damage, and also provide nutrition for cells. The sage extract, with components such as rosmarinic acid, strongly scavenges reactive oxygen species, inhibits the inflammatory signaling pathway, and creates a stable cell microenvironment. The bioactive peptide can reshape the extracellular matrix and activate the cell proliferation and repair signaling pathway. The three act synergistically at multiple targets to promote cell repair.

[0009] In some embodiments, the ceramide composition is composed of the following raw materials in parts by weight: 1-1.5 parts of rice bran oil powder, 0.5-1 part of sage extract, and 10-27 parts of bioactive peptide.

[0010] In some embodiments, the ceramide composition is composed of the following raw materials in parts by weight: 1.2 parts of rice bran oil powder, 0.8 part of sage extract, and 15 parts of bioactive peptide.

[0011] In some embodiments, the bioactive peptide is fish collagen peptide, bird's nest peptide, and sea cucumber peptide. Preferably, the weight ratio of the fish collagen peptide, bird's nest peptide, and sea cucumber peptide is 30-55:1:1-10; more preferably 35-50:1:3-8; most preferably 40:1:5.

[0012] In some embodiments, the mass percentage of ceramide in the rice bran oil powder is 1%-3%.

[0013] In some embodiments, the molecular weight of the fish collagen peptide is 1000-5000 Da.

[0014] In a second aspect, the present invention provides a preparation method of the above ceramide composition, including the following steps: mixing the rice bran oil powder, sage extract, and bioactive peptide.

[0015] In a third aspect, the present invention provides the application of the above ceramide composition in the preparation of a food capable of promoting cell repair.

[0016] In a fourth aspect, the present invention provides a food capable of promoting cell repair, including the above ceramide composition and food additives.

[0017] The food additives in the present invention refer to synthetic or natural substances added to food to improve the quality, color, aroma, and taste of food, as well as for anti-corrosion, preservation, and processing needs. Food flavorings, base substances in gum-based candies, processing aids for the food industry, and nutritional fortifiers are also included. For example, the food additive is at least one of a sweetening agent, a flavor enhancer, an acidity regulator, a flour treatment agent, a preservative, a filler, a flavor correcting agent, a coloring agent, an antioxidant, a thickening agent, a stabilizer, an emulsifier, an anti-caking agent, a flow aid, a moisture-holding agent, a leavening agent, and a lubricant.

[0018] The beneficial effects of the present invention are as follows: 1. Through the synergistic effect among rice bran oil powder, sage extract, and bioactive peptides, the present invention jointly promotes cell repair and achieves efficient repair of the skin barrier. Rice bran oil powder contains oryzanol, phytosterols, and ceramide analogs, which can strengthen the cell membrane structure, resist free radical damage, and provide nutrition for cells. Sage extract, with components such as rosmarinic acid, strongly scavenges reactive oxygen species, inhibits the inflammatory signaling pathway, and creates a stable cell microenvironment. Bioactive peptides can reshape the extracellular matrix and activate the cell proliferation and repair signaling pathways. The three act synergistically at multiple targets to promote cell repair.

[0019] 2. The ceramides composition in the present invention contains plant-derived ceramides. Due to its naturalness and skin affinity, the prepared ceramides composition is greener, safer, and has higher bioavailability. Compared with animal-derived or biosynthetic ceramides, it has the characteristics of mildness and low irritation, and is more suitable for the repair of sensitive skin or damaged barriers.

[0020] 3. The ceramides used in the present invention are derived from rice bran oil, which is a by-product of rice processing. Due to its rich fatty acids, it has become a high-quality oil resource in China. By integrating resources for efficient utilization, it can reduce the raw material cost, maintain high activity and performance value during food preparation applications, and has high edible value. Description of the Drawings

[0021] Figure 1 It is a scratch map of the surface of Hacat cells in different groups.

[0022] Figure 2 It is a statistical chart of the migration rate of Hacat cells after treatment in different groups. Among them, compared with the blank control group, ***P < 0.001, ****P < 0.0001, and ns indicates no significant difference compared with the blank control group, P > 0.05. Detailed Embodiments

[0023] The following description of the embodiments is only used to help understand the method and its core idea of the present invention. It should be noted that for those of ordinary skill in the art of this technology, without departing from the principle of the present invention, several improvements and modifications can be made to the present invention, and these improvements and modifications also fall within the protection scope of the claims of the present invention. The following description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be obvious to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but can be applied to a wider range consistent with the principles and novel features disclosed herein. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present invention belongs.

[0024] Next, embodiments and comparative examples of the present invention are shown to describe the liquid beverage of the present invention in more detail. However, the present invention is not limited thereto. Unless otherwise specified, the percentages in the present invention are all mass percentages.

[0025] The present invention does not limit the source of the raw materials used. Unless otherwise specified, the raw materials used in the present invention are all ordinary commercially available products in this technical field.

[0026] The rice bran oil powder described in the present invention is a conventional food in this field and can be prepared by conventional methods in this field. For example, rice bran oil is extracted, filtered, diluted with dextrin, pasteurized, and spray-dried to obtain rice bran oil powder; the fish collagen peptide meets the requirements of SB / T 10634-2011 Freshwater Fish Collagen Peptide Powder and can be prepared by conventional methods in this field. For example, fish scales / fish skins are washed, enzymatically hydrolyzed, filtered, decolorized and deodorized, concentrated, sterilized, and spray-dried to obtain fish collagen peptide powder.

[0027] The following are the sources of raw materials for illustrative purposes: rice bran oil powder, purchased from Hongcheng (Shanghai) Health Technology Co., Ltd.; sage extract, purchased from Guangzhou Kanglai Food Additive Co., Ltd.; fish collagen peptide, purchased from Hubei Ruibang Biotechnology Co., Ltd., with an average molecular weight of about 1000 Da; bird's nest peptide, purchased from Hubei Ruibang Biotechnology Co., Ltd.; sea cucumber peptide, purchased from Zhejiang Nuoyan Biotechnology Co., Ltd.

[0028] Examples 1 - 5 and Comparative Examples 1 - 3 For the ceramide composition, according to Table 1, mix the raw materials in the formulated amounts evenly.

[0029] Table 1

[0030] Note: 1. The dosage unit in the table is parts by weight; 2. A:B:C in the table is the weight ratio of fish collagen peptide, bird's nest peptide and sea cucumber peptide; 3. " / " in the table means the dosage is 0.

[0031] Detection of scratch migration rate of Hacat cells a) Inoculate the Hacat cell suspension in a 12-well plate and culture it in an incubator at 37°C and 5% CO2 until the cell density reaches 95%-100%; b) When the cell density reaches 95%-100%, start scratching and administering drugs. Before scratching, prepare the compositions of each example and comparative example with serum-free MEM medium, and remove the microorganisms in the sample by microfiltration to avoid bacterial contamination; c) Discard the old medium, use the tip of a 200 μL pipette tip to make scratches perpendicular to the cell well plate, and make three vertical scratches in each well; d) Wash 1-2 times with PBS to remove the cell debris clumps generated by scratching; e) Place the cell well plate under a microscope to observe and photograph the initial scratch; f) Set up blank control group, positive control group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group in the experiment; g) Add 1 mL of serum-free MEM medium to the first well in the first column of the 12-well plate as the blank control group; add 1 mL of serum-containing MEM medium to the second well in the second column as the positive control group; add 1 mL of the prepared compositions of each example and comparative example to the remaining wells as each example group and comparative example group; h) After adding the drugs, place them in an incubator at 37°C and 5% CO2 for 24 hours; i) Aspirate the medium in each well, observe and photograph the scratch after 24 hours of drug addition under a microscope.

[0032] Use Image J software to analyze the width of each scratch and calculate the scratch migration rate. Scratch migration rate = (S0H - S24H) / S0H * 100%, where: S0H is the scratch length at 0 hour, and S24H is the scratch length at 24 hours.

[0033] By creating wounds by scratching the cell surface and comparing the changes in the scratch width after 24 h, the effects of each treatment group on the migration ability of Hacat cells were explored. The results are as follows Figure 1 and Figure 2 shown.

[0034] The results showed that there were significant differences in the migration rates of Hacat cells between the groups of Examples 1 - 5 and the blank control group (P < 0.05), indicating that the ceramide compositions prepared in Examples 1 - 5 could significantly increase the migration rate of Hacat cells and promote cell repair.

[0035] There were no significant differences in the migration rates of Hacat cells between the groups of Comparative Examples 1 - 3 and the blank control group (P > 0.05), indicating that the ceramide compositions prepared in Comparative Examples 1 - 3 could not promote the migration of Hacat cells and could not promote cell repair. It can be seen that at the same administration concentration, neither the composition of rice bran oil powder and sage extract nor the single bioactive peptide could promote the migration of Hacat cells. At the same time, the combination of rice bran oil powder, sage extract and bioactive peptide with a conventional dosage relationship could not promote the migration of Hacat cells either. However, the combination of rice bran oil powder, sage extract and bioactive peptide with a specific dosage relationship according to the present invention could significantly promote the migration and fusion of Hacat cells, had a good skin barrier repair effect, and achieved an unexpected technical effect.

[0036] The above is a further description of the present invention in combination with specific embodiments, but these embodiments are merely exemplary and do not constitute any limitation to the scope of the present invention. Those skilled in the art should understand that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the protection scope of the present invention.

Claims

1. A ceramide composition capable of promoting cell repair, characterized in that, It is composed of the following raw materials in parts by weight: 0.5 - 2 parts of rice bran oil powder, 0.5 - 2 parts of sage extract, and 5 - 32 parts of bioactive peptides; the bioactive peptides are fish collagen peptides, bird's nest peptides, and sea cucumber peptides, and the weight ratio of the fish collagen peptides, bird's nest peptides, and sea cucumber peptides is 30 - 55:1:1 - 10.

2. The ceramide composition according to claim 1, wherein It is composed of the following raw materials in parts by weight: 1 - 1.5 parts of rice bran oil powder, 0.5 - 1 part of sage extract, and 10 - 27 parts of bioactive peptides.

3. The ceramide composition according to claim 1, characterized in that, It is composed of the following raw materials in parts by weight: 1.2 parts of rice bran oil powder, 0.8 part of sage extract, and 15 parts of bioactive peptides.

4. The ceramide composition according to claim 1, wherein The weight ratio of the fish collagen peptides, bird's nest peptides, and sea cucumber peptides is 35 - 50:1:3 - 8.

5. The ceramide composition according to claim 1, characterized in that, The weight ratio of the fish collagen peptides, bird's nest peptides, and sea cucumber peptides is 40:1:

5.

6. The ceramide composition according to claim 1, wherein The mass percentage of ceramide in the rice bran oil powder is 1% - 3%.

7. The ceramide composition according to claim 1, wherein The molecular weight of the fish collagen peptide is 1000 - 5000 Da.

8. A method for preparing the ceramide composition according to any one of claims 1-7, characterized in that, It includes the following steps: mixing the rice bran oil powder, sage extract, and bioactive peptides.

9. Use of the ceramide composition according to any one of claims 1 - 7 in the preparation of a food capable of promoting cell repair.

10. A food that can promote cell repair, characterized in that, It includes the ceramide composition according to any one of claims 1 - 7 and a food additive.

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