Method for reactivating stored aerobic granular sludge by AHLs combination

Abstract

The invention discloses a method for reviving stored aerobic granular sludge by AHLs combination, and belongs to the field of sewage treatment.The method comprises the steps that the stored aerobic granular sludge is mixed with reviving liquid containing the AHLs combination, then the mixed liquid is subjected to reviving culture under the aerobic condition, and finally the revived aerobic granular sludge is applied to a sewage treatment system again. The method is simple and easy to implement, the activity and stability of the aerobic granular sludge can be effectively recovered, and the sewage treatment efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of sewage treatment, and in particular to a method for reviving stored aerobic granular sludge with a combination of AHLs. Background Art

[0002] Aerobic granular sludge is an efficient biological treatment technology, which has good sedimentation performance, high biological activity and the ability to remove pollutants. However, aerobic granular sludge is prone to losing its activity and stability during storage, resulting in a decline in its treatment efficiency.

[0003] N-acylhomoserinelactones (AHLs) are a class of signal molecules widely present in bacteria, and they play an important role in intercellular communication and quorum sensing. In the aerobic granular sludge technology, AHLs are also considered an important regulatory factor that can affect the formation and stability of aerobic granular sludge. By adding AHLs externally, the cost of aerobic granular sludge can be reduced. This is because AHLs are simple chemical substances, and their synthesis and production costs are relatively low, and the cost of aerobic granular sludge can be reduced through large-scale production and commercial application.

[0004] Currently, in aerobic granular sludge, aerobic granular sludge is a complex ecosystem composed of various microorganisms, and different microorganisms may synthesize different types of AHLs. The concentration of AHLs may be affected by the number and activity of microorganisms. If the number of microorganisms is small or their activity is low, then the concentration of AHLs may be low, thus affecting their role in intercellular communication and quorum sensing. Appropriate types, concentrations and ratios of AHLs are an important research topic for reviving stored aerobic granular sludge. Summary of the Invention

[0005] The purpose of the present invention is to provide a method for reviving stored aerobic granular sludge with a combination of AHLs, so as to improve its activity and stability, and thus improve the sewage treatment efficiency.

[0006] To achieve the above object, the present invention is implemented according to the following technical solution:

[0007] The present invention includes the following steps:

[0008] S1: Mix the aerobic granular sludge with a recovery solution containing a combination of AHLs;

[0009] S2: Place the mixed solution under aerobic conditions for recovery culture;

[0010] S3: Reapply the revived aerobic granular sludge to the sewage treatment system.

[0011] Preferably, the concentration of the AHLs combination in the resuscitation solution is 20 - 100 nM. The conditions for resuscitation culture are a temperature of 20 - 35 °C, a dissolved oxygen concentration of 5 - 8 mg / L, and a culture time of 1 - 4 days.

[0012] Furthermore, the AHLs combination includes one or more of C6 - HSL, C8 - HSL, C10 - HSL, and C12 - HSL. The molar concentration ratio of C6 - HSL, C8 - HSL, C10 - HSL, and C12 - HSL is 1:1:1:1, 1:1:1:0, 1:1:0:0, or 1:0:0:0; the 1:1:1:1 represents equal molar concentrations of C6 - HSL, C8 - HSL, C10 - HSL, and C12 - HSL, the 1:1:1:0 represents equal molar concentrations of any three of C6 - HSL, C8 - HSL, C10 - HSL, and C12 - HSL; the 1:1:0:0 represents equal molar concentrations of any two of C6 - HSL, C8 - HSL, C10 - HSL, and C12 - HSL; the 1:0:0:0 represents one of C6 - HSL, C8 - HSL, C10 - HSL, and C12 - HSL.

[0013] Specifically, the sewage treatment system is an aerobic granular sludge process treatment device including an inlet, a reaction tank, a sedimentation tank, and an outlet part.

[0014] Specifically, the aerobic granular sludge is prepared through steps of inoculating sludge, adding carriers, microbial inoculants, the aerobic granular sludge process, and storage.

[0015] Specifically, the resuscitation solution is prepared by dissolving the AHLs combination in methanol.

[0016] The beneficial effects of the present invention are as follows:

[0017] The present invention is a method for reviving stored aerobic granular sludge with an AHLs combination. Compared with the prior art, by adding the resuscitation solution and performing resuscitation culture, the present invention can significantly improve the activity and stability of aerobic granular sludge, thereby improving the sewage treatment efficiency. This method is simple to operate and has low costs, and is applicable to various aerobic granular sludge processes. Description of the Drawings

[0018] Figure 1 is the effect diagram of the aerobic granular sludge in Example 1 of the present invention;

[0019] Figure 2 is the effect diagram of the aerobic granular sludge in Example 2 of the present invention;

[0020] Figure 3 is the effect diagram of the aerobic granular sludge in Example 3 of the present invention;

[0021] Figure 4 It is the effect diagram of the aerobic granular sludge in Example 4 of the present invention. Specific Embodiments

[0022] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. The illustrative embodiments and descriptions of the present invention are used to explain the present invention, but not to limit the present invention.

[0023] Example 1:

[0024] Step 1: Mix the stored aerobic granular sludge with a resuscitation solution containing C6-HSL, C8-HSL, C10-HSL, and C12-HSL in a ratio of 1:1:1:1 and a concentration of 20 nM;

[0025] Step 2: Place the mixed solution under the conditions of a temperature of 30 °C and a dissolved oxygen concentration of 5 mg / L for resuscitation culture for 1 day;

[0026] Step 3: Reapply the resuscitated aerobic granular sludge to the sewage treatment system.

[0027] Take out the aerobic granular sludge and test its performance. The COD, EPS, and ATP results are listed in Table 1, and the SVI, MLSS, and MLVSS results are listed in Table 2. The effect diagram of the aerobic granular sludge is Figure 1 .

[0028] Example 2:

[0029] Step 1: Mix the stored aerobic granular sludge with a resuscitation solution containing C8-HSL, C10-HSL, and C12-HSL in a ratio of 1:1:1 and a concentration of 40 nM;

[0030] Step 2: Place the 4 kinds of mixed solutions under the conditions of a temperature of 35 °C and a dissolved oxygen concentration of 8 mg / L for resuscitation culture for 4 days;

[0031] Step 3: Reapply the resuscitated aerobic granular sludge to the sewage treatment system.

[0032] Take out the aerobic granular sludge and test its performance. The COD, EPS, and ATP results are listed in Table 1, and the SVI, MLSS, and MLVSS results are listed in Table 2. The effect diagram of the aerobic granular sludge is Figure 2 .

[0033] Example 3:

[0034] Step 1: Mix the stored aerobic granular sludge with a resuscitation solution containing C8-HSL and C10-HSL in a ratio of 1:1 and a concentration of 80 nM;

[0035] Step 2: Place the mixture under the conditions of a temperature of 25°C and a dissolved oxygen concentration of 6 mg / L for resuscitation culture, and the culture time is 2 days;

[0036] Step 3: Reapply the resuscitated aerobic granular sludge to the sewage treatment system, and the sewage treatment efficiency is significantly improved.

[0037] Take out the aerobic granular sludge and test its performance. The COD, EPS, and ATP results are listed in Table 1, and the SVI, MLSS, and MLVSS results are listed in Table 2. The effect diagram of the aerobic granular sludge is Figure 3 .

[0038] Example 4:

[0039] Step 1: Mix the stored aerobic granular sludge with a resuscitation solution containing C12-HSL at a concentration of 100 nM;

[0040] Step 2: Place the mixture under the conditions of a temperature of 30°C and a dissolved oxygen concentration of 7 mg / L for resuscitation culture, and the culture time is 3 days;

[0041] Step 3: Reapply the resuscitated aerobic granular sludge to the sewage treatment system, and the sewage treatment efficiency is significantly improved.

[0042] Take out the aerobic granular sludge and test its performance. The COD, EPS, and ATP results are listed in Table 1, and the SVI, MLSS, and MLVSS results are listed in Table 2. The effect diagram of the aerobic granular sludge is Figure 4 .

[0043] Table 1

[0044]

[0045]

[0046] Table 2

[0047]

[0048] The technical solution of the present invention is not limited to the limitations of the above specific embodiments. Any technical deformation made according to the technical solution of the present invention falls within the protection scope of the present invention.

Claims

1. A method for reviving stored aerobic granular sludge with a combination of AHLs, characterized in that, It includes the following steps: S1: Mix aerobic granular sludge with a resuscitation solution containing a combination of AHLs; S2: Place the mixed solution under aerobic conditions for resuscitation culture; S3: Reapply the resuscitated aerobic granular sludge to the sewage treatment system.

2. The method for resuscitating the stored aerobic granular sludge using the AHLs combination according to claim 1, characterized in that: The concentration of the AHLs combination in the resuscitation solution is 20 - 100 nM.

3. The method for reviving stored aerobic granular sludge with the AHLs combination according to claim 2, wherein: The AHLs combination includes one or more of C6-HSL, C8-HSL, C10-HSL, and C12-HSL.

4. The method for reviving stored aerobic granular sludge with the AHLs combination according to claim 3, wherein: The molar concentration ratio of C6-HSL, C8-HSL, C10-HSL, and C12-HSL is 1:1:1:1, 1:1:1:0, 1:1:0:0, or 1:0:0:0; 1:1:1:1 means equal molar concentrations of C6-HSL, C8-HSL, C10-HSL, and C12-HSL, 1:1:1:0 means equal molar concentrations of any three of C6-HSL, C8-HSL, C10-HSL, and C12-HSL; 1:1:0:0 means equal molar concentrations of any two of C6-HSL, C8-HSL, C10-HSL, and C12-HSL; 1:0:0:0 means one of C6-HSL, C8-HSL, C10-HSL, and C12-HSL.

5. The method for resuscitating stored aerobic granular sludge using the AHLs combination according to claim 1, wherein: The conditions for the resuscitation culture are a temperature of 20 - 35 °C, a dissolved oxygen concentration of 5 - 8 mg / L, and a culture time of 1 - 4 days.

6. The method for reviving the stored aerobic granular sludge using the AHLs combination according to claim 1, wherein: The sewage treatment system is an aerobic granular sludge process treatment device including an influent, a reaction tank, a sedimentation tank, and an effluent part.

7. The method for reviving stored aerobic granular sludge with the AHLs combination according to claim 1 or 6, characterized in that: The aerobic granular sludge is prepared through steps of inoculating sludge, adding carriers, microbial inoculants, the aerobic granular sludge process, and storage.

8. The method for reviving the stored aerobic granular sludge using the AHLs combination according to claim 1, characterized in that: The resuscitation solution is prepared by dissolving the AHLs combination in methanol.

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