A strain of *Morchella mosierifolia* and its application in promoting crop growth and enhancing crop stress resistance.

Abstract

This invention discloses a strain of *Tetranychus mosieurii* and its application in promoting crop growth and enhancing crop stress resistance, relating to the field of screening strains related to crop growth promotion. The strain is deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC NO. 40677. Under salt stress, inoculation with this strain reduced sodium content in tomato stems, leaves, and roots by 16.9% and 20.7%, respectively. Furthermore, plant biomass, nitrogen, phosphorus, and potassium uptake, and root morphology were essentially on par with the salt-free control, indicating that this strain almost reversed the harmful effects of 100 mM NaCl salt stress on tomatoes. Simultaneously, this strain also significantly promotes cucumber root growth and nutrient absorption rate, root biomass, total root length, total root surface area, total root volume, and root activity.

Description

Technical Field

[0001] This invention relates to the field of screening technology for crop growth-promoting strains, and more specifically to a strain of *Mammillaria mosierifolia* and its application in promoting crop growth and improving crop stress resistance. Technical Background

[0002] my country possesses abundant brackish water resources. According to incomplete statistics, the area of ​​brackish groundwater in northern my country is approximately 1.38 million km². 2 Under arid conditions, irrigation with brackish water is of great significance in alleviating the water crisis. Tomato (Lycopersicon esculentum Miller) is one of the world's most important vegetables, with a total cultivation area of ​​approximately 200,000 hectares in China. 2 It also has a certain degree of salt tolerance and has the potential to effectively utilize brackish water resources.

[0003] Microorganisms play a crucial role in mitigating salt stress in plants, enhancing their resilience and tolerance, repairing damaged soil, and restoring the ecosystem. Currently, various fungi have been confirmed to enhance crop salt tolerance. Among them, arbuscular mycorrhizal fungi (AMF) are an ancient class of microorganisms that can form symbiotic relationships with most plants, thereby promoting plant growth and stress resistance. However, different strains have varying effects. Irrigating tomatoes with slightly saline water and applying highly efficient microbial strains to reduce salt stress has significant potential for saving substantial amounts of freshwater resources.

[0004] Cucumber (Cucumis sativus L.) is one of the major cultivated vegetable crops in my country. However, its shallow root system, weak absorption capacity, and low drought resistance and stress resistance restrict the stable and high yield of cucumbers.

[0005] Therefore, how to provide a microbial strain that promotes crop growth and enhances crop resistance, and apply it to promote salt tolerance in tomatoes and root growth and nutrient absorption in cucumbers, is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0006] In view of this, the present invention provides a strain of *Benmoses styracifolium* and its application in promoting crop growth and improving crop stress resistance.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] A strain of *Funneliformis mosseae* has been deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC NO.40677, deposited on July 3, 2023, at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

[0009] Uses of *M. moses*, including the following uses;

[0010] 1) Application in promoting crop nutrient absorption and increasing yield;

[0011] 2) Application in improving crop resistance to environmental stress.

[0012] Preferably, the crops include tomatoes and cucumbers.

[0013] Preferably, the environmental stress is salt stress.

[0014] Preferably, the resistance to environmental stress manifests as follows:

[0015] 1) Moses tube-stalk sclerotium reversed plant cell membrane damage, reduced the degree of plasma membrane peroxidation, and decreased the MDA content in plants under salt stress.

[0016] 2) Moses tube-stalk sclerotium increased the content of superoxide dismutase (SOD) in plants under salt stress;

[0017] 3) Moses tube-stalk cysticercosis upregulated Na + Expression of the efflux gene SOS1.

[0018] A microbial preparation comprising the aforementioned *Morchella mosierifolia*, said microbial preparation being used to improve crop growth and enhance the crop's resistance to salt stress.

[0019] As can be seen from the above technical solution, compared with the prior art, the beneficial effects of the present invention are as follows:

[0020] This invention screened a strain of *Tetranychus mosierifolius*, and inoculation with this strain improved the ability of tomatoes to antagonize salt stress. Under salt stress (100 mM NaCl), the sodium content in tomato stems, leaves, and roots decreased by 16.9% and 20.7%, respectively. Plant biomass, nitrogen, phosphorus, and potassium uptake, and root morphology were all at levels comparable to the salt-free control, indicating that this strain almost completely reversed the harmful effects of 100 mM NaCl salt stress on tomatoes. Inoculation with this strain reduced salt damage in tomatoes from a physiological and molecular biological perspective, reversed cell membrane damage, and increased antioxidant enzyme activity; it also upregulated the expression of tomato genes SlAMT1.2 and SlPT4, the vacuolar membrane aquaporin gene TIP, and the Na+ efflux gene SOS1. This strain can improve the ability of tomatoes to antagonize salt stress, reverse membrane damage, and increase antioxidant enzyme activity, laying the foundation for subsequent development of salt-stress-resistant microbial agents.

[0021] Meanwhile, this strain also significantly promoted root growth and nutrient absorption in cucumber production, increasing root biomass, total root length, total root surface area, total root volume, and root activity by 38.8%, 62.4%, 50.5%, 43.2%, and 6.6%, respectively. Root nitrogen, phosphorus, and potassium absorption increased by 47.8%, 70.3%, and 55.8%, respectively. Cucumber yield increased by 6.9%. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0023] Figure 1 The spore morphology characteristics of the strain of this invention; Figure 1 A: Magnified 10 times in PLVG solution; Figure 1 B: Magnified 50 times in PLVG solution; Figure 1 C: Melzer reagent magnified 100 times;

[0024] Figure 2 Phylogenetic tree construction for the SSU-ITS-LSU sequence of the strain of this invention;

[0025] Figure 3 The effect of the strain of this invention on the root morphology of tomatoes under brackish water irrigation; Figure 3 A: Effects of bacterial strain on total root length of tomatoes under slightly saline irrigation; Figure 3 B: The effect of strain on the total root surface area of ​​tomato roots under slightly saline water irrigation; Figure 3 C: The effect of strain on the average diameter of tomato roots under slightly saline irrigation; Figure 3 D: Total root volume of tomato under slightly saline irrigation by the strain; CK is the uninoculated control, NYN is the strain treatment; in the same block, the letters a and b indicate significant differences at the 5% level (n=4), with an error of SE;

[0026] Figure 4 The effects of the strain of this invention on proline, soluble sugar, malondialdehyde (MDA), and superoxide dismutase (SOD) in tomatoes under slightly brackish water irrigation. Figure 4 A: The effect of bacterial strains on proline in tomatoes under slightly brackish water irrigation; Figure 4 B: Effect of the strain on soluble sugars in tomatoes under slightly saline irrigation; Figure 4 C: Effect of the strain on malondialdehyde (MDA) in tomatoes under brackish water irrigation; Figure 4 D: Effect of the strain on superoxide dismutase (SOD) in tomatoes under slightly saline irrigation; CK is the uninoculated control, and NYN is the strain treatment; within the same block, the letters a and b indicate significant differences at the 5% level (n=3), with an error of SE. Detailed Implementation

[0027] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0028] Example 1

[0029] Isolation, screening and identification of strains

[0030] 1. Isolation and screening of strains

[0031] This strain was derived from the topsoil of the rhizosphere of *Setaria viridis* in the northwestern desert of Inner Mongolia, China (39°19′N, 109°57′E). The specific isolation method was as follows: Topsoil samples from 0 to 10 cm depth were collected using a 5 cm soil auger. After air-drying, 50 g of the air-dried soil sample was weighed and placed on the top of the analyzer's sieve set (sieve apertures from top to bottom: 5 mm, 2 mm, 1 mm, 0.5 mm, 0.25 mm, 50 μm). Deionized water was slowly added along the barrel wall until the soil sample was submerged. The sample was soaked and moistened for 5 minutes, then vertically shaken for 5 minutes at 25-0 times / min with an amplitude of 3 cm. The material remaining on the 50 μm sieve was collected, and single spores were extracted under a microscope using tweezers.

[0032] 2. Identification of strains

[0033] 1) Morphological identification

[0034] The extracted spores were pressed into slides to determine their morphological characteristics. Specifically, AMF spore slides and spore wall samples were prepared using polyvinyl alcohol-lactic acid glycerol mixture (PVLG) and Melzer's reagent, respectively. The microstructure of AMF spores was examined under an Olympus DP72 compound microscope to determine the morphological characteristics of the spores. The color of fresh specimens was examined under a 20-micron dissecting microscope.

[0035] Spore morphological characteristics (see) Figure 1 ):

[0036] Spore color: yellow to brown

[0037] Spore shape and size: spherical or only spherical, (94.41-)171.55(-307.35)μm

[0038] Spore wall: There are three layers of spore wall: SWL1, SWL2, and SWL3.

[0039] SWL1 characteristics: semi-flexible, semi-transparent, stains reddish-brown in Melzer reagent, with a thickness of (1.16-)3.4(-8.51)μm; often degrades to form a granular layer that falls off in mature spores, and appears granular in the late stage of degradation.

[0040] SWL2 characteristics: permanent, usually rigid, breaks into smooth, flaky fragments when separated from SWL3, does not react in Melzer reagent, and has a thickness of (1.16-)2.45(-4.91)μm.

[0041] SWL3 characteristics: uniform, permanent, usually yellow to yellowish-brown, separable from the lower surface of SWL2 in crushed spores, with a thickness of (1.15-)2.04(-3.37) μm.

[0042] Melzer staining: SWL1 is stained reddish-brown in Melzer reagent, while SWL2-3 layers are left unstained.

[0043] Other characteristics: the germination pores are (10.81-)16.59(-24.4) μm wide at the top, with septa commonly seen below, forming a funnel shape together, and the spores contain an oily substance.

[0044] 2) Molecular biological identification

[0045] DNA was extracted from the selected strains using a DNA extraction kit (CTAB Plant Genomic DNA Rapid Extraction Kit, Adley Company). The SSU-ITS-LSU sequence was amplified to obtain the sequence shown in SEQ ID NO.1. After BLAST alignment, a phylogenetic tree was constructed by comparing the SSU-ITS-LSU sequence of the strain with sequences of species with high homology in the NCBI database. The phylogenetic tree showed that the strain of this invention and *Tetranychus mosierifolius* belong to the same clade and are genetically closest (see...). Figure 2 ).

[0046] Example 2

[0047] Effects of *Mammillaria mosierifolia* on tomato biomass, nitrogen, phosphorus, potassium uptake, and sodium ion content under brackish water irrigation

[0048] The experiment used a control group with no salt damage (0 mmol·L⁻¹). -1 (0mM) and salt damage 100mmol· -1 NaCl (100 mM) treatment was performed in six replicates. Six seeds were sown per pot, and after germination, three seedlings of uniform growth were retained. Each seedling in the treatment group was inoculated with 50 spores of this strain. Salt stress treatment began two weeks later. Salt was applied via irrigation with the nutrient solution, with saturated irrigation every three days. At the end of the experiment, the NaCl concentration in the leachate from the 100 mM treatment was [data missing]. + The content is 4.5 g·L -1 .

[0049] Methods for determining growth and mineral nutrient uptake indicators: Biomass was measured as oven-dried weight; nitrogen was determined by the Kjeldahl method after digestion of dried plant samples; phosphorus was determined by the molybdenum-antimony colorimetric method; potassium and sodium contents were determined by flame spectrophotometry. Root morphology was scanned using a Perfection V800 Photo scanner (Seiko Epson, Japan) and analyzed using the WinRHIZO 2007 system. All data were analyzed by ANOVA and SPSS variance analysis, with a significance level of 5%.

[0050] Table 1 shows that at a salt concentration of 100 mM, the average weight of a single tomato plant without inoculation and salt stress treatment was 1.03 g, which was 21.97% lower than that of the control plant without salt stress (1.32 g). However, when this strain was inoculated under salt stress conditions, the weight of a single plant was 1.24 g, which was not significantly different from the control, indicating that the 100 mM salt stress was reversed in terms of biomass.

[0051] Table 1. Effects of this strain on tomato biomass under brackish water irrigation.

[0052]

[0053] Note: In the same block, the letters a, b, and c indicate that there is a significant difference at the 5% level (n=6), and the error value is SD.

[0054] Inoculation with this strain increased nitrogen, phosphorus, and potassium uptake in tomato plants under salt stress, providing more nutrients for plant growth and improving their salt tolerance (Tables 2, 3, 4). Inoculation with this strain significantly promoted the uptake of nitrogen, phosphorus, and potassium in tomatoes under salt stress. Total nitrogen uptake increased from 25.80 mg in the control to 33.82 mg, phosphorus from 7.42 mg to 11.00 mg, and potassium from 63.96 mg to 84.35 mg, representing increases of 31.08%, 48.25%, and 31.88%, respectively, all approaching the levels of the salt-free control. This indicates that this strain reversed the salt damage caused by 100 mM NaCl in slightly brackish water in terms of nutrient uptake.

[0055] Table 2. Effects of this strain on nitrogen content and uptake in tomatoes under brackish water irrigation.

[0056]

[0057] Note: In the same block, the letters a, b, and c indicate that there is a significant difference at the 5% level (n=6), and the error value is SD.

[0058] Table 3. Effects of this strain on phosphorus content and uptake in tomatoes under brackish water irrigation.

[0059]

[0060] Note: In the same block, the letters a, b, and c indicate that there is a significant difference at the 5% level (n=6), with an error of SD.

[0061] Table 4. Effects of this strain on potassium content and uptake in tomatoes under brackish water irrigation.

[0062]

[0063] Note: In the same block, the letters a, b, and c indicate that there is a significant difference at the 5% level (n=6), with an error of SD.

[0064] Table 5. Effects of this strain on sodium content and uptake in tomatoes under brackish water irrigation.

[0065]

[0066] Note: In the same block, the letters a, b, and c indicate that there is a significant difference at the 5% level (n=6), with an error of SD.

[0067] Na + It is a monovalent cation that can be absorbed by crops; it is K. + The main competitor of ions, but K+ The function cannot be used by Na + Substitution and a decrease in the K:Na ratio can disrupt the structure and function of the membrane, thereby exacerbating the damage caused by salt stress to plants. Therefore, maintaining an appropriate homeostatic K:N ratio is crucial. Under salt stress, inoculation with this strain increased this ratio from 1.92 (63.96:33.39) to 2.53 (84.35:33.33) (Tables 3 and 4), an increase of 31.77%.

[0068] Inoculation with this strain results in the formation of a mycelial network at the plant roots, absorbing water and nutrients, increasing the surface area for root absorption of mineral elements, and inhibiting sodium absorption. + Excessive accumulation of Na+ in plants reduces Na+ levels. + Sodium content. In this invention, tomatoes were irrigated with slightly saline water and inoculated with this strain, resulting in a 16.9% reduction in sodium content in stems and leaves and a 20.7% reduction in sodium content in roots (Table 5). This indicates that this strain significantly enhances the ability of tomatoes to antagonize salt stress.

[0069] According to the experimental results of this embodiment, inoculation with this strain also improved the root morphology of tomatoes. Under salt stress, the total root length, total root surface area, average diameter, and total root volume decreased significantly. However, after inoculation with this strain, the total root length, total root surface area, average diameter, and total root volume increased significantly by 39.69%, 28.07%, 20.00%, and 100.00%, respectively. Figure 3 Under salt stress, this strain expands the plant's absorption range by improving plant nutrition levels and altering root morphology.

[0070] Example 3

[0071] The effects of *Benmoses styracifolium* on salt tolerance physiology in tomatoes under brackish water irrigation

[0072] The experiment used a control group with no salt damage (0 mol·L⁻¹). -1 (0mM) and salt treatment 100mmol·L -1 NaCl (100mM), 6 replicates.

[0073] The proline content of tomato plants under different treatments was determined using the sulfosalicylic acid-toluene absorbance method. The results are as follows: Figure 4 A showed that under 100 mM salt stress, the proline content in tomato plants increased to 17.17 mg / g DW (dry weight). This indicates that under salt stress, the decrease in cell water potential induces proline accumulation to balance the high intracellular salt content. Inoculation with this strain under salt stress resulted in a 17.26% decrease in proline content, indicating that the inoculated strain improved the plant's salt tolerance and reduced proline synthesis and accumulation caused by salt stress.

[0074] The soluble sugar content in the leaves of tomato plants under different treatments was determined by anthrone method colorimetry, and the results are as follows: Figure 4 B shows that under 100mM salt stress, the soluble sugar content in tomato stems and leaves increased after inoculation with this strain, and was close to that under salt-free conditions, indicating that this strain can regulate plant salt tolerance by increasing soluble sugar.

[0075] The malondialdehyde (MDA) content in tomato plants under different treatments was determined using the phosphate buffer-thiobarbituric acid method. The results are as follows: Figure 4 C indicates that malondialdehyde (MDA) is the main end product of membrane lipid peroxidation. Under salt stress, the MDA content in tomato leaves is directly proportional to the salt concentration; the higher the salt concentration, the higher the MDA content in the plant. At salt concentrations of 0 mM and 100 mM, the MDA content in plant leaves was 1.60 and 2.57 μmol / g, respectively. Inoculation with this strain under salt-free conditions had no significant effect on leaf MDA; however, at a salt concentration of 100 mM, inoculation with this strain significantly reduced the MDA content by 37.74%, reaching the same level as under salt-free conditions. This indicates that inoculation with this strain reversed the effects of salt on plant cell membrane damage and reduced the degree of plasma membrane peroxidation, suggesting that plants under the conditions of this strain experience less oxidative damage.

[0076] The superoxide dismutase (SOD) content in the stems and leaves of tomato plants under different treatments was determined by the dismutation reaction of catalytic anion free radicals and the NBT (nitroblue tetrazolium) photoreduction method. The results are as follows: Figure 4 D showed that the SOD activity in tomato stems and leaves increased with increasing irrigation water salt concentration. Under 0 and 100 mM NaCl treatments, the changes in leaf SOD were significant, being 1.87 times and 2.92 times that of the salt-free control, respectively. This indicates that the plant's response to salt damage involves increased antioxidant enzyme activity, scavenging reactive oxygen species within the plant, reducing membrane peroxidation, and enhancing the plant's salt resistance. Inoculation with this strain under salt-damage treatment increased plant SOD by 26.9%, demonstrating that inoculation significantly improved the plant's salt tolerance.

[0077] Example 4

[0078] Effects of *Benmoses styracifolium* on nitrogen and phosphorus transport and salt tolerance-related gene expression in tomatoes under brackish water irrigation

[0079] The relative expression levels of genes related to nitrogen and phosphorus uptake and salt tolerance at the transcriptional level were analyzed using real-time quantitative PCR. RNA was extracted from plant roots using a kit from Tiangen Biotech (Beijing) Co., Ltd., and reverse transcribed into cDNA using a kit from Beijing Adley Biotechnology Co., Ltd. The resulting products were used for real-time quantitative PCR analysis. Ubiquitin genes were used as internal reference genes, and their relative expression levels were calculated according to Equation 2-ΔΔCt.

[0080] The AMT family of genes consists of high-affinity ammonium nitrogen transporter genes, and AMT1.2 is a member of the AMT family. Under salt-free conditions, the expression level of the AMT1.2 gene in tomatoes inoculated with this strain was upregulated. The PT gene is a type of phosphorus transporter gene, and PT4 is closely related to mycorrhizal infection. Under salt-free conditions, the expression of the PT4 gene in tomatoes inoculated with this strain was upregulated, indicating efficient phosphorus transport. Under salt-induced conditions, both AMT1.2 and PT4 genes in tomatoes inoculated with this strain were upregulated, indicating that the strain combats salt damage by improving nitrogen and phosphorus uptake in plants. The aquaporin gene TIP was significantly upregulated, indicating that inoculation with this strain improved the absorption of harmful Na+. + The ability to isolate into vacuoles.

[0081] Na in vacuole + / H + Downregulation of the antitransfer protein (NHX) gene indicates that the plant's absorption of Na... + The amount has decreased. SOS1 is Na + The efflux gene, SOS1, was upregulated after inoculation of this strain under saline conditions, indicating that mycorrhizal inoculation promoted the efflux of Na+ ions.

[0082] Table 6. Effects of this strain on nitrogen and phosphorus translocation and salt tolerance gene expression in tomatoes under brackish water irrigation.

[0083]

[0084] Note: In the same block, the letters a, b, and c indicate that there is a significant difference at the 5% level (n=4), with an error of SD.

[0085] Example 5

[0086] The effects of *Benmoses pedunculata* on cucumber growth and yield

[0087] A field trial was conducted using 10 cucumber varieties: Jingyan 20-s90, Jingyan Mini, Jingyan Dongmei, 8-21, Mili 62, Beinong Jiaxiu, Jingyan Mini 9, Zhongnong 49, Mini 2118, and Zhongnong 59. Treatments included inoculation with the strain, while the control was uninoculated. The trial was conducted with four replicates. Each plot was 8 square meters, and the plant spacing was 25 x 50 cm. The cucumber's growth period was 115 days.

[0088] The results are shown in Tables 7 and 8. Inoculation with this strain had a significant impact on nutrient absorption, root morphology, and yield of cucumber (Cucumis sativus L.). Table 7 shows that the average biomass of cucumber roots differed significantly. Inoculation with arbuscular mycorrhizal fungi increased the nitrogen, phosphorus, and potassium contents of the roots by 5.79%, 17.15%, and 14.12%, respectively; and the total uptake increased by 47.8%, 70.3%, and 55.8%, respectively.

[0089] Table 7. Effects of this strain on cucumber root nutrition.

[0090]

[0091]

[0092] Note: In the same block, the letters a, b, and c indicate that there is a significant difference at the 5% level (n=4), with an error of SD.

[0093] Plant root morphology determines the contact area with soil and its absorption capacity; a good root morphology facilitates the plant's acquisition of water and nutrients from the soil. This strain not only altered the root morphology of cucumbers but also significantly increased the average root biomass. Inoculation with this strain increased cucumber root dry weight by 38.8%, and in terms of root morphology, total root length increased by 62.4%, total root volume increased by 50.5%, and total root surface area increased by 43.2% (Table 8), significantly increasing root activity. The absorption of nitrogen, phosphorus, and potassium in cucumber roots is closely related to cucumber root activity. The experimental results showed that the yield per plant was 5.8 kg in the control group and 6.2 kg in the inoculated strain group, an increase of 6.9%.

[0094] Table 8. Effects of this strain on cucumber root morphology.

[0095]

[0096] Note: In the same block, the letters a, b, and c indicate that there is a significant difference at the 5% level (n=4), with an error of SD.

[0097] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0098] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A type of *M. moses* ( ) Funneliformis mosseae ) strain, characterized in that, The strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC NO.40677, deposited on July 3, 2023, at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

2. The use of *M. moses* as described in claim 1, characterized in that, The intended use is as follows; 1) Application in promoting cucumber growth and absorption of mineral nutrients (nitrogen, phosphorus, potassium) and increasing yield; 2) Application in improving the tolerance of tomatoes to salt stress.

3. The use according to claim 2, characterized in that, The tolerance to salt stress is as follows: 1) Moses tube-stalk sclerotium reversed tomato cell membrane damage, reduced plasma membrane peroxidation, and decreased MDA content in plants under salt stress. 2) Moses tuberculosis increased the content of superoxide dismutase (SOD) in tomatoes under salt stress; 3) Moses tube stalk cysts upregulated the expression of the Na+ efflux gene SOS1.

4. A microbial preparation, characterized in that, Includes *M. mossiostomata* as described in claim 1; the microbial preparation is used to improve cucumber growth and enhance the ability of tomatoes to resist salt stress.

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