Microfluidic sampling device capable of quickly and accurately positioning liver cancer tumor biomarkers

Abstract

The invention discloses a microfluidic sampling device capable of quickly and accurately positioning a liver cancer tumor biomarker, and relates to the technical field of medical instruments, the microfluidic sampling device comprises a chip mounting part, the chip mounting part is formed by assembling a bottom assembly disc, a microfluidic chip, a top plate and an assembly rod, and the microfluidic chip is arranged at the upper part of the bottom assembly disc; through accurate cooperation of the air pressure push rod and an external control system and flow adjustment of a valve control hole in the micro-fluidic chip, it is ensured that liquid inlet conditions for each detection are highly consistent, the detection stability is greatly improved, waste liquid generated in the detection process flows into a waste bin through a sample outlet micro-fluidic channel and a liquid outlet micro-fluidic channel, and the detection efficiency is improved. Due to the fact that the waste bin is independently arranged and is effectively isolated from untreated sample and reagent space, residual pollutants in waste liquid cannot diffuse or leak to other areas, cross contamination between samples is avoided, and specificity and sensitivity of liver cancer tumor biomarker detection are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and more specifically to a microfluidic sampling device capable of quickly and accurately locating biomarkers of liver cancer tumors. Background Art

[0002] Microfluidics is a technology for precisely controlling and manipulating fluids at the microscale. Specifically, it integrates basic operation units such as sample preparation, reaction, separation, and detection in a biological, chemical, or medical analysis process onto a microfluidic chip with an area of a few square centimeters, and automatically completes the entire analysis process. Due to its great potential in the fields of biology, chemistry, and medicine, it has developed into a new research field that intersects multiple disciplines such as biology, chemistry, medicine, fluidics, electronics, materials, and machinery.

[0003] When the existing microfluidic sampling devices are used for detecting biomarkers of liver cancer tumors, the traditional liquid inlet method often relies on manual operation, making it difficult to accurately control the liquid inlet speed, liquid inlet volume, and liquid inlet timing. As a result, the flow rate of the sample and reagent fluctuates significantly when entering the microfluidic chip, the injection volume is difficult to be stabilized within the set threshold, and it is difficult for different components to be mixed orderly according to the preset program. This instability in the liquid inlet process directly interferes with the chemical reaction process between the biomarker and the detection reagent, thereby affecting the generation and amplification of the detection signal, and ultimately leading to deviations in the detection results, reducing the accuracy and reliability of the detection.

[0004] On the other hand, the inconvenience in the waste liquid treatment link mainly stems from the fact that the device's structural design does not fully consider the independent collection and effective isolation of waste liquid. The existing microfluidic sampling devices usually lack an optimized layout specifically for waste liquid collection and storage. After the waste liquid is generated, it is often in a space close to or connected to the untreated sample and reagent, lacking effective physical separation and an independent discharge channel. This allows pollutants such as residual biomolecules and chemical reagents in the waste liquid to easily diffuse and leak through the microchannels or tiny gaps on the surface of the device, cross-contact with the samples being detected or to be detected, causing cross-contamination between samples, and thereby interfering with subsequent detection reactions, resulting in false positives or false negatives in the detection results, seriously affecting the specificity and sensitivity of the detection of biomarkers of liver cancer tumors. Therefore, the present invention proposes a microfluidic sampling device capable of quickly and accurately locating biomarkers of liver cancer tumors. Summary of the Invention

[0005] The object of the present invention is to provide a microfluidic sampling device that can quickly and accurately locate liver cancer tumor biomarkers, so as to solve the problems in the above-mentioned background technology that the traditional liquid inlet mode often relies on manual operation, and it is difficult to accurately control the liquid inlet speed, liquid inlet volume, and liquid inlet timing. This results in large fluctuations in the flow rate of the sample and reagent when entering the microfluidic chip, the injection volume is difficult to be stabilized within the set threshold, and it is difficult for different components to be mixed orderly according to the preset program. The instability of this liquid inlet process will directly interfere with the chemical reaction process between the biomarker and the detection reagent, thereby affecting the generation and amplification of the detection signal, and ultimately leading to deviations in the detection results, reducing the accuracy and reliability of the detection.

[0006] On the other hand, the inconvenience in the waste liquid treatment link mainly stems from the fact that the device's structural design does not fully consider the independent collection and effective isolation of waste liquid. Existing microfluidic sampling devices usually lack an optimized layout specifically for waste liquid collection and storage. After the waste liquid is generated, it is often in a space close to or connected to the untreated sample and reagent, lacking effective physical separation and independent discharge channels. This allows contaminants such as residual biomolecules and chemical reagents in the waste liquid to easily diffuse and leak through the microchannels or tiny gaps on the surface of the device, making cross-contact with the samples being detected or to be detected, causing cross-contamination between samples, and thus interfering with subsequent detection reactions, resulting in false positives or false negatives in the detection results, seriously affecting the specificity and sensitivity of liver cancer tumor biomarker detection.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A microfluidic sampling device that can quickly and accurately locate liver cancer tumor biomarkers, including a chip mounting member, which is assembled by a bottom assembly plate, a microfluidic chip, a top plate, and an assembly rod. The microfluidic chip is installed on the upper part of the bottom assembly plate. The bottom of the microfluidic chip is assembled with a top plate, and an assembly rod is installed on the top of the top plate. A support rod is annularly arranged on the upper end surface of the top plate, and the ends of several support rods are commonly connected to an installation plate. An air pressure push rod is fixedly installed on the top of the installation plate, and the output end of the air pressure push rod penetrates through the installation plate and is connected to a pressing plate. The bottom of the pressing plate is provided with a liquid inlet part, and several groups of the liquid inlet parts are annularly arranged along the microfluidic chip. A sampling part is detachably connected to the side of several groups of the liquid inlet parts.

[0009] Optionally, the bottom assembly plate includes a first assembly plate, a second assembly plate, and a chassis. The first assembly plate is located between the second assembly plate and the chassis, and the first assembly plate is located above the second assembly plate.

[0010] Optionally, a convex opening is provided at the center of the first assembly disc, and a plurality of fan-shaped grooves are distributed in the ring portion of the first assembly disc. A limiting block is provided between each of the plurality of fan-shaped grooves, and a slot is provided in a ring between the convex opening and the fan-shaped grooves on the first assembly disc.

[0011] Optionally, a circular hole adapted to the convex opening is provided in the middle of the second assembly disc. A plurality of chip mounting grooves corresponding to the fan-shaped grooves are provided on the upper end surface of the second assembly disc. Microfluidic chips are mounted on each of the plurality of chip mounting grooves. Plug blocks adapted to the plurality of slots are distributed at the bottom of the second assembly disc.

[0012] Optionally, the upper part of the chassis is in interference fit with the assembly rod through an assembly key.

[0013] Optionally, a waste bin is independently provided on each of the plurality of fan-shaped grooves, and a communication hole communicating with the pipeline of the microfluidic chip is provided on the side of the waste bin.

[0014] Optionally, the microfluidic chip includes a sample inlet, a liquid inlet, and a sample inlet microfluidic channel, a liquid inlet microfluidic channel, a sample outlet microfluidic channel, and a liquid outlet microfluidic channel which are correspondingly communicated and arranged on the microfluidic chip. A first valve control hole and a second valve control hole are respectively provided on the sample inlet microfluidic channel and the liquid inlet microfluidic channel. The sample outlet microfluidic channel and the liquid outlet microfluidic channel communicate with the waste bin.

[0015] Optionally, the liquid inlet part includes a column provided on the top of the top plate. A plurality of bearing plates are connected to the top of the column through a mounting frame. A syringe is fixedly installed on the side end surface of the bearing plate through a mounting sleeve. The piston rod body of the syringe is fixedly connected to the bottom of the extrusion disc. A clamp for clamping the head of the syringe is provided below the mounting sleeve. The output end of the syringe faces the liquid inlet.

[0016] Optionally, a clamping groove is provided on one side of the bearing plate. A clamping block is movably clamped on the clamping groove. An extension rod is provided outward on one side of the clamping block. A mounting seat is provided at the bottom of the extension rod through a connecting rod. A sample storage tank is installed in the mounting seat. The top of the sample storage tank is connected with a sampling hose through a connecting cap, and a sampling needle is provided at the bottom of the sample storage tank. The sampling needle faces the sample inlet.

[0017] Optionally, the sampling hose (19) is connected to an external sample.

[0018] The beneficial effects of the present invention are:

[0019] 1. In the present invention, through the precise coordination of the pneumatic push rod and the external control system, as well as the flow rate adjustment of the valve control holes on the microfluidic chip, the liquid inlet conditions for each detection are ensured to be highly consistent, greatly improving the stability of the detection. The waste liquid generated during the detection process flows into the waste bin through the sample outlet microfluidic channel and the liquid outlet microfluidic channel. Since the waste bin is independently arranged and effectively isolated from the untreated sample and reagent space, the residual pollutants in the waste liquid cannot diffuse or leak into other areas, avoiding cross-contamination between samples. The specificity and sensitivity of the detection of liver cancer tumor biomarkers are improved.

[0020] 2. When the pneumatic push rod in the present invention pushes the extrusion disc downward, the reagent in the syringe can directly and accurately enter the liquid inlet of the microfluidic chip without a complex adjustment process. Moreover, the sampling part is movably clamped to the card slot on the bearing plate through the clamping block. During installation, only need to pull the clamping block upward from the bottom of the card slot and push it to the top to achieve complete clamping in place. Since the card slot is pre-precisely set, the installed sampling part is naturally directly above the sample inlet, ensuring that the sampling needle can accurately insert into the sample inlet, realizing rapid and accurate sampling of the sample. This simple clamping installation method not only improves the assembly efficiency, but also ensures high-precision alignment between the sampling part and the sample inlet. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is a schematic structural diagram of the microfluidic sampling device for rapidly and accurately positioning liver cancer tumor biomarkers of the present invention;

[0022] Figure 2 is a schematic structural diagram of the sampling part in the present invention;

[0023] Figure 3 is a schematic structural diagram of the chip mounting part in the present invention;

[0024] Figure 4 is a schematic structural diagram of the back of the chip mounting part in the present invention;

[0025] Figure 5 is an exploded view of the microfluidic chip in the present invention;

[0026] Figure 6 is an exploded view of the microfluidic chip from another perspective in the present invention;

[0027] Figure 7 is a schematic plan view of the microfluidic chip in the present invention.

[0028] The reference numerals in the figures are:

[0029] 1. Chip mounting part;

[0030] 101. Bottom assembly plate; 1011. First assembly plate; 10111. Convex port; 10112. Slot; 10113. Sector slot; 10114. Limit block; 1012. Second assembly plate; 10121. Chip mounting groove; 10122. Insert block; 1013. Chassis; 10131. Assembly key;

[0031] 102. Microfluidic chip; 1021. Sampling inlet; 10211. Sampling microfluidic channel; 10212. First valve control hole; 10213. Sampling outlet microfluidic channel; 1022. Liquid inlet; 10221. Liquid inlet microfluidic channel; 10222. Second valve control hole; 10223. Liquid outlet microfluidic channel;

[0032] 103. Top plate; 104. Assembly rod; 105. Waste bin; 1051. Communication hole;

[0033] 2. Support rod; 3. Mounting plate; 4. Pneumatic push rod; 5. Extrusion plate; 6. Column; 7. Mounting frame; 8. Bearing plate; 801. Card slot; 9. Mounting sleeve; 10. Clamp; 11. Syringe; 12. Block; 13. Extension rod; 14. Connecting rod; 15. Mounting seat; 16. Sample storage tank; 17. Connecting cap; 18. Sampling needle; 19. Sampling hose. Detailed implementation manners

[0034] To make the technical means, creative features, achieved purposes and functions of the present invention easy to understand, the present invention will be further described below in conjunction with specific implementation manners.

[0035] The following description is made in conjunction with the preferred embodiments of the device of the present invention.

[0036] Embodiment 1:

[0037] As shown in Appendix Figure 1 to Appendix Figure 7 As shown, the present invention provides a microfluidic sampling device capable of quickly and accurately positioning liver cancer tumor biomarkers, including a chip mounting member 1, which is assembled by a bottom assembly plate 101, a microfluidic chip 102, a top plate 103 and an assembly rod 104. The microfluidic chip 102 is installed on the upper part of the bottom assembly plate 101, the bottom of the microfluidic chip 102 is assembled with a top plate 103, the top of the top plate 103 is provided with an assembly rod 104, the upper end surface of the top plate 103 is annularly provided with support rods 2, the ends of several support rods 2 are commonly connected to a mounting plate 3, the top of the mounting plate 3 is fixedly installed with a pneumatic push rod 4, the output end of the pneumatic push rod 4 penetrates through the mounting plate 3 and is connected to an extrusion plate 5, the bottom of the extrusion plate 5 is provided with a liquid inlet part, the liquid inlet part is annularly provided with several groups along the microfluidic chip 102, and a sampling part is detachably connected to the side of several groups of liquid inlet parts.

[0038] As described above, the pneumatic push rod 4 is precisely controlled by an external control system, which can accurately adjust the telescopic speed and displacement of the pneumatic push rod 4 according to a preset program. When the detection process is started, the external control system sends an instruction to the pneumatic push rod 4, and the output end of the pneumatic push rod 4 begins to push down the extrusion disc 5. During the downward movement of the extrusion disc 5, a uniform pressure is applied to the liquid inlet part. By precisely adjusting the parameters of the pneumatic push rod 4, such as air pressure, telescopic speed, and stroke, etc., the accurate control of the liquid inlet speed and liquid inlet volume can be achieved. At the initial stage of detection, it is necessary to slowly inject the sample. At this time, the telescopic speed of the pneumatic push rod 4 can be reduced to make the sample enter the microfluidic chip 102 at a smaller flow rate, ensuring that the sample can fully infiltrate the microfluidic channel. In the subsequent reagent addition stage, according to the characteristics of the reagent and reaction requirements, the parameters of the pneumatic push rod 4 can be adjusted to make the reagent enter the microfluidic chip 102 at an appropriate speed and amount, realizing the mixing of different components according to the preset time sequence. This precise liquid inlet control provides a stable and controllable environment for the subsequent biochemical reactions, ensuring that the reactions can proceed as expected and improving the accuracy and reliability of the detection.

[0039] Embodiment 2:

[0040] As shown in the Figure 5 to the Figure 7 accompanying drawings, this embodiment is basically the same as the previous embodiment, except that the bottom assembly disc 101 includes a first assembly disc 1011, a second assembly disc 1012, and a chassis 1013. The first assembly disc 1011 is located between the second assembly disc 1012 and the chassis 1013, and the first assembly disc 1011 is located above the second assembly disc 1012.

[0041] Furthermore, a convex mouth 10111 is provided at the center of the first assembly disc 1011. A number of fan-shaped grooves 10113 are distributed on the ring part of the first assembly disc 1011. Limiting blocks 10114 are provided between the fan-shaped grooves 10113. A slot 10112 is provided in a ring between the convex mouth 10111 and the fan-shaped grooves 10113 on the first assembly disc 1011.

[0042] Furthermore, a circular hole adapted to the convex mouth 10111 is provided in the middle of the second assembly disc 1012. A number of chip mounting grooves 10121 corresponding to the fan-shaped grooves 10113 are provided on the upper end surface of the second assembly disc 1012. Microfluidic chips 102 are mounted on the chip mounting grooves 10121. Insert blocks 10122 cooperating with the slots 10112 are distributed at the bottom of the second assembly disc 1012.

[0043] Furthermore, the upper part of the chassis 1013 is in interference fit with the assembly rod 104 through an assembly key 10131.

[0044] Furthermore, waste bins 105 are independently provided on several sector-shaped grooves 10113, and a communication hole 1051 communicating with the pipeline of the microfluidic chip 102 is formed in the side of the waste bin 105.

[0045] Furthermore, the microfluidic chip 102 includes a sample inlet 1021, a liquid inlet 1022, and a sample inlet microfluidic channel 10211, a liquid inlet microfluidic channel 10221, a sample outlet microfluidic channel 10213, and a liquid outlet microfluidic channel 10223 that are correspondingly communicated and arranged on the microfluidic chip 102. First valve control holes 10212 and second valve control holes 10222 are respectively arranged on the sample inlet microfluidic channel 10211 and the liquid inlet microfluidic channel 10221. The sample outlet microfluidic channel 10213 and the liquid outlet microfluidic channel 10223 are communicated with the waste bin 105.

[0046] Furthermore, the microfluidic chip 102 is coated with a transparent substrate.

[0047] As can be seen from the above, waste bins 105 are independently provided on several sector-shaped grooves 10113, and each waste bin 105 corresponds to a microfluidic chip 102, realizing the independent collection of waste liquid. A communication hole 1051 communicating with the pipeline of the microfluidic chip 102 is formed in the side of the waste bin 105. During the detection process, the waste liquid generated in the microfluidic chip 102 flows into the waste bin 105 through the pipeline via the communication hole 1051. Since each waste bin 105 is independent and effectively isolated from the untreated sample and reagent space, pollutants such as residual biomolecules and chemical reagents in the waste liquid cannot diffuse or leak to other areas, avoiding cross-contamination between samples.

[0048] Specifically, pick up the second assembly plate 1012, align its round hole with the convex mouth 10111 of the first assembly plate 1011, and slowly lower the second assembly plate 1012 to make the round hole and the convex mouth 10111 fit preliminarily. At this time, the chip installation groove 10121 of the second assembly plate 1012 should roughly correspond to the fan-shaped groove 10113 of the first assembly plate 1011. Take out the microfluidic chip 102, carefully pick up the edge of the chip with tweezers, avoid touching the microfluidic channels and interfaces on the chip, and prevent damage to the chip. Align the microfluidic chip 102 with the chip installation groove 10121 on the second assembly plate 1012, and slowly lower the chip so that the chip falls smoothly into the chip installation groove 10121. Place each waste bin 105 on the corresponding fan-shaped groove 10113 of the first assembly plate 1011. After placement, due to the limitation of the fan-shaped groove 10113, the communication hole 1051 of the waste bin 105 is automatically aligned with the pipeline outlet of the microfluidic chip 102. Then align the assembly key 10131 on the chassis 1013 with the assembly rod 104, and slowly push the chassis 1013 downward to make the assembly key 10131 and the assembly rod 104 achieve interference fit. During the pushing process, a certain resistance may be felt, which is due to the characteristics of interference fit. At this time, apply force evenly to ensure that the chassis 1013 is installed in place smoothly.

[0049] Furthermore, for the sealing test: An airtightness detection device can be used to introduce gas with a certain pressure into the sample inlet 1021 and the liquid inlet 1022 of the microfluidic chip 102, and observe the change in the reading of the pressure gauge and whether there is gas leakage at each connection part. If the reading of the pressure gauge remains stable within the specified time and no bubbles emerge from the connection part, it indicates that the device has good sealing performance; if the pressure drops significantly or there is gas leakage, it is necessary to check the connection part, find the leakage point and repair it.

[0050] For the microfluidic channel patency test: Inject a small amount of test liquid into the microfluidic chip 102, and observe the flow of the liquid in the microfluidic channels through a microscope. Under normal circumstances, the liquid should be able to flow smoothly through each channel without blockage or poor flow. If it is found that the liquid stagnates or flows slowly somewhere, it indicates that the channel at that place may be blocked, and special tools are needed to clean it.

[0051] Example Three:

[0052] As shown in the appendix Figure 1 to the appendix Figure 2As shown, this embodiment is basically the same as the previous embodiment, except that the liquid inlet portion includes a column 6 arranged on the top of the top plate 103, the top of the column 6 is connected to a plurality of supporting plates 8 through a mounting frame 7, the side end surface of the supporting plate 8 is fixedly mounted with a syringe 11 through a mounting sleeve 9, the piston rod of the syringe 11 is fixedly connected to the bottom of the extrusion disk 5, and a clamp 10 for clamping the head of the syringe 11 is provided below the mounting sleeve 9, and the output end of the syringe 11 is directly opposite to the liquid inlet 1022.

[0053] A card slot 801 is provided on one side of the supporting plate 8, and a card block 12 is movably connected to the card slot 801. An extension rod 13 is provided outward on one side of the card block 12. A mounting seat 15 is provided at the bottom of the extension rod 13 through a connecting rod 14. A sample storage tank 16 is installed in the mounting seat 15. The top of the sample storage tank 16 is connected to a sampling hose 19 through a connecting cap 17, and an injection needle 18 is provided at the bottom of the sample storage tank 16. The injection needle 18 is opposite to the injection port 1021, and the sampling hose 19 is connected to the external sample.

[0054] As can be seen from the above, when installing the sampling part, it is only necessary to pull the card block 12 upward from the bottom of the card slot 801, and slowly push the card block 12 into the card slot 801 until the card block 12 is moved to the top of the card slot 801 to achieve complete snapping into place. Since the card slot 801 is pre-set, the installed sampling part is directly above the sampling port 1021.

[0055] Specifically, when the pneumatic push rod 4 pushes the extrusion disk 5 downward, the reagent in the syringe 11 can directly and accurately enter the liquid inlet 1022 of the microfluidic chip 102, without the need for a complicated adjustment process, which greatly improves the liquid inlet efficiency and positioning accuracy. The sampling part is movably connected to the card slot 801 on the carrier plate 8 through the card block 12. During installation, it is only necessary to pull the card block 12 upward from the bottom of the card slot 801 and push it to the top to achieve complete card connection. Since the card slot 801 is accurately set in advance, the sampling part after installation is naturally directly above the injection port 1021, ensuring that the injection needle 18 can be accurately inserted into the injection port 1021, thereby achieving rapid and accurate injection of the sample. This simple card-connected installation method not only improves assembly efficiency, but also ensures high-precision alignment between the sampling part and the injection port 1021.

[0056] The above shows and describes the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions merely illustrate the principles of the present invention. Various changes and modifications may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and modifications are intended to fall within the scope of the present invention. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. A microfluidic sampling device capable of quickly and accurately positioning liver cancer tumor biomarkers, characterized in that: Comprising a chip mounting member (1), the chip mounting member (1) is assembled by a bottom mounting plate (101), a microfluidic chip (102), a top plate (103) and a mounting rod (104). The microfluidic chip (102) is installed on the upper part of the bottom mounting plate (101). The bottom of the microfluidic chip (102) is assembled with a top plate (103). The top of the top plate (103) is provided with a mounting rod (104). The upper end surface of the top plate (103) is provided with a support rod (2) in a ring shape. The ends of a number of the support rods (2) are jointly connected to a mounting plate (3). The top of the mounting plate (3) is fixedly installed with a pneumatic push rod (4). The output end of the pneumatic push rod (4) penetrates through the mounting plate (3) and is connected to a pressing plate (5). The bottom of the pressing plate (5) is provided with a liquid inlet part. The liquid inlet part is provided with a number of groups in a ring shape along the microfluidic chip (102). A sampling part is detachably connected to the side of each of the number of groups of the liquid inlet parts.

2. The microfluidic sampling device for quickly and accurately locating liver cancer tumor biomarkers according to claim 1, wherein: The bottom mounting plate (101) includes a first mounting plate (1011), a second mounting plate (1012) and a chassis (1013). The first mounting plate (1011) is located between the second mounting plate (1012) and the chassis (1013). The first mounting plate (1011) is located on the upper part of the second mounting plate (1012).

3. The microfluidic sampling device for quickly and accurately locating liver cancer tumor biomarkers according to claim 2, wherein: A convex opening (10111) is provided at the central part of the first mounting plate (1011). A number of fan-shaped grooves (10113) are distributed in a ring shape on the first mounting plate (1011). A limiting block (10114) is provided between each of the number of fan-shaped grooves (10113). A slot (10112) is provided in a ring shape on the first mounting plate (1011) between the convex opening (10111) and the fan-shaped grooves (10113).

4. The microfluidic sampling device for quickly and accurately locating liver cancer tumor biomarkers according to claim 2, wherein: A circular hole adapted to the convex opening (10111) is opened at the middle part of the second mounting plate (1012). A number of chip mounting grooves (10121) corresponding to the fan-shaped grooves (10113) are provided on the upper end surface of the second mounting plate (1012). The microfluidic chip (102) is installed on each of the number of chip mounting grooves (10121). A number of insertion blocks (10122) adapted to the number of slots (10112) are distributed at the bottom of the second mounting plate (1012).

5. The microfluidic sampling device for rapidly and accurately locating liver cancer tumor biomarkers according to claim 2, wherein: The upper part of the chassis (1013) is in interference fit with the mounting rod (104) through a mounting key (10131).

6. The microfluidic sampling device for quickly and accurately locating liver cancer tumor biomarkers according to claim 3, wherein: A waste bin (105) is independently provided on each of the number of fan-shaped grooves (10113). A communication hole (1051) communicating with the pipeline of the microfluidic chip (102) is opened at the side part of the waste bin (105).

7. The microfluidic sampling device for quickly and accurately locating liver cancer tumor biomarkers according to claim 1, characterized in that: The microfluidic chip (102) includes a sample inlet (1021), a liquid inlet (1022), and a sample inlet microfluidic channel (10211), a liquid inlet microfluidic channel (10221), a sample outlet microfluidic channel (10213), and a liquid outlet microfluidic channel (10223) that are correspondingly connected and disposed on the microfluidic chip (102). A first valve control hole (10212) and a second valve control hole (10222) are respectively provided on the sample inlet microfluidic channel (10211) and the liquid inlet microfluidic channel (10221). The sample outlet microfluidic channel (10213) and the liquid outlet microfluidic channel (10223) are connected to a waste bin (105).

8. The microfluidic sampling device for quickly and accurately locating liver cancer tumor biomarkers according to claim 1, wherein: The liquid inlet part includes a column (6) provided on the top of the top plate (103). A plurality of carrier plates (8) are connected to the top of the column (6) through a mounting bracket (7). A syringe (11) is fixedly installed on the side end face of the carrier plate (8) through a mounting sleeve (9). The piston rod body of the syringe (11) is fixedly connected to the bottom of the extrusion disc (5). A clamp (10) for clamping the head of the syringe (11) is provided below the mounting sleeve (9). The output end of the syringe (11) is directed at the liquid inlet (1022).

9. The microfluidic sampling device for quickly and accurately locating liver cancer tumor biomarkers according to claim 8, wherein: A card slot (801) is formed on one side of the carrier plate (8). A card block (12) is movably clamped on the card slot (801). An extension rod (13) is provided outward on one side of the card block (12). A mounting seat (15) is provided at the bottom of the extension rod (13) through a connecting rod (14). A sample storage tank (16) is installed in the mounting seat (15). The top of the sample storage tank (16) is connected to a sampling hose (19) through a connecting cap (17). An inlet needle (18) is provided at the bottom of the sample storage tank (16). The inlet needle (18) is directed at the sample inlet (1021).

10. The microfluidic sampling device for rapidly and accurately locating liver cancer tumor biomarkers according to claim 9, wherein: The sampling hose (19) is connected to an external sample.

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