Cell shaking culture device

Abstract

The invention discloses a cell shaking culture device, and relates to the technical field of cell culture, the cell shaking culture device comprises a case, a shaking plate is arranged in the case, a driving mechanism is arranged in the case, the driving end of the driving mechanism is connected with the shaking plate and drives the shaking plate to shake back and forth, a lower layer elastic net is arranged in the shaking plate, and a lower layer elastic net is arranged in the lower layer elastic net. A plurality of stand columns are arranged on the swing plate, a lifting frame is arranged on the stand columns in a lifting and sliding mode, an upper-layer elastic net is arranged in the lifting frame, and the upper-layer elastic net is located over the lower-layer elastic net. By utilizing the lifting frame, when the conical flask is limited, the lifting frame slides upwards firstly, the lifting frame drives the upper-layer elastic net to slide upwards, then the conical flask is placed on the lower-layer elastic net of the rocking plate, finally, the lifting frame slides downwards, the lifting frame drives the upper-layer elastic net to slide downwards, and the top of the conical flask penetrates through a limiting opening of the upper-layer elastic net; and therefore, the conical flask can be conveniently limited.

Description

Technical Field

[0001] The present application relates to the field of cell culture technology, and in particular to a cell oscillation culture device. Background Art

[0002] Cell culture is a technique that simulates the in vivo environment by placing cells in a suitable culture medium to allow them to grow, proliferate, and differentiate. By continuously or intermittently shaking the culture vessel, cells are kept in suspension. This culture method improves the even distribution of nutrients and oxygen, promoting cell growth.

[0003] Currently, the cell oscillation culture device includes an incubator, which is equipped with a drive mechanism and a shaker. The drive mechanism drives the shaker to shake on the horizontal plane, thereby culturing the cells. Two layers of elastic nets are installed at intervals along the vertical direction of the shaker. Each layer of elastic net is composed of two groups of elastic ropes. Each group of multiple elastic ropes is evenly spaced and arranged in parallel. The two groups of elastic ropes are perpendicular to each other on the horizontal plane and form an elastic net. A rectangular limit opening is formed between two adjacent elastic ropes in one group and two adjacent elastic ropes in the other group. Multiple limit openings are evenly spaced on a layer of elastic net. The elastic net is used to limit the cell culture container so that the culture container is not easily shaken during the shaking of the shaker.

[0004] Cell culture containers are typically test tubes or Erlenmeyer flasks. When using a test tube for cell culture, the tube is vertically inserted into the two retaining openings of the two layers of elastic mesh to retain the tube. When using an Erlenmeyer flask for cell culture, the bottom of the flask is passed through the retaining opening of the upper elastic mesh and placed on the lower elastic mesh. The retaining opening of the upper elastic mesh is now located in the upper half of the flask, and the upper elastic mesh cooperates with the lower elastic mesh to retain the flask in place.

[0005] When limiting the position of the conical flask, since the diameter of the bottom of the conical flask is larger than the diameter of the top of the conical flask, the diameter of the bottom of the conical flask will be much larger than the space of the limiting opening. Therefore, it will be difficult for the bottom of the conical flask to pass through the limiting opening of the upper elastic net, making it difficult to limit the conical flask. Summary of the Invention

[0006] In order to facilitate the positioning of a conical flask, the present application provides a cell oscillation culture device.

[0007] The cell oscillation culture device provided in this application adopts the following technical solution:

[0008] A cell oscillation culture device includes a chassis, a rocking plate is provided in the chassis, a driving mechanism is provided in the chassis, a driving end of the driving mechanism is connected to the rocking plate and drives the rocking plate to rock back and forth, a lower elastic net is provided in the rocking plate, a plurality of columns are provided on the rocking plate, a lifting frame is provided on the plurality of columns for lifting and sliding, an upper elastic net is provided in the lifting frame, and the upper elastic net is located directly above the lower elastic net.

[0009] By adopting the above technical solution, when limiting the position of the conical flask, first slide the lifting frame upward, the lifting frame drives the upper elastic net to slide upward, then place the conical flask on the lower elastic net of the rocking plate, and finally slide the lifting frame downward, the lifting frame drives the upper elastic net to slide downward, and the top of the conical flask passes through the limiting opening of the upper elastic net, thereby facilitating the limiting of the conical flask.

[0010] Preferably, slides are provided on the two adjacent inner side walls of the lifting frame for sliding in the horizontal direction, and the upper elastic net includes multiple first elastic ropes and second elastic ropes, the two ends of the first elastic ropes are respectively fixedly connected to the inner wall of the lifting frame and a slide, the multiple first elastic ropes are parallel to each other, the two ends of the second elastic ropes are respectively fixedly connected to the inner wall of the lifting frame and another slide, the multiple second elastic ropes are parallel to each other, and the first elastic ropes and the second elastic ropes are perpendicular to each other, a driving rack is provided on the two columns, and two driving assemblies are provided in the lifting frame, the driving assembly is transmission-connected to the driving rack and the slide, and when the lifting frame moves downward, the driving rack drives the slide to move in the lifting frame through the driving assembly.

[0011] By adopting the above technical solution, when the lifting frame drives the upper elastic net downward, if the first elastic cord or the second elastic cord is located directly above the mouth of the conical flask, the first elastic cord or the second elastic cord will press down on the mouth of the conical flask, preventing the mouth of the conical flask from entering the restricted opening of the upper elastic net. When the lifting frame moves downward, the two drive racks drive the two slides within the lifting frame through the two drive assemblies. The two slides respectively drive one end of the first elastic cord and the second elastic cord to move, causing the first elastic cord and the second elastic cord that are pressing down on the mouth of the conical flask to deflect, thereby facilitating the mouth of the conical flask to enter the restricted opening of the upper elastic net.

[0012] Preferably, the driving assembly includes a driving shaft, a driving gear, a driven gear, a driven rack and a non-return component. The driving shaft is rotatably set in the lifting frame, the driving gear is rotatably set on the driving shaft and meshes with the driving rack, the driven rack is fixedly set at the end of the slide, the driven gear is fixedly set on the driving shaft and meshes with the driven rack, and the non-return component is set at the connection between the driving shaft and the driving gear. When the lifting frame moves upward, the driving rack drives the driving gear to idle on the driving shaft. When the lifting frame moves downward, the non-return component locks the driving gear and the driving shaft, and the driving gear drives the driving shaft to rotate.

[0013] By adopting the above technical solution, when the lifting frame moves upward, the driving rack drives the driving gear to rotate in the forward direction, and the driving gear rotates on the driving shaft but does not drive the driving shaft to rotate, so that the slide plate does not move. When the lifting frame moves downward, the driving rack drives the driving gear to rotate in the reverse direction. At this time, the anti-return component locks the driving gear and the driving shaft, so that the driving gear drives the driving shaft to rotate, the driving shaft drives the driven gear to rotate, the driven gear drives the driven rack to rotate, and the driven rack drives the slide plate to slide, thereby causing the first elastic rope and the second elastic rope to be offset.

[0014] Preferably, the anti-return component includes multiple pawls and elastic reset members, multiple pawls are rotatably arranged on the outer wall of the drive shaft, multiple elastic reset members are arranged at the rotational connection between multiple pawls and the drive shaft, a ratchet groove is opened on the side wall of the drive gear, and multiple pawls are located in the ratchet groove.

[0015] By adopting the above technical solution, when the driving rack drives the driving gear to rotate forward, the driving gear rotates through multiple pawls. When the driving rack drives the driving gear to rotate reversely, the pawls are clamped in the ratchet grooves, thereby driving the driving shaft to rotate.

[0016] The two ends of the driving shaft are detachably mounted on the two front ends of the driving member, and the two ends of the driving member are detachably mounted on the two front ends of the driving member.

[0017] By adopting the above technical solution, when the lifting frame is on the rocking plate, the second bracket is inserted into the lifting frame, and the driving shaft is located in the second claw. When the lifting frame rises and moves from the rocking plate, the lifting frame drives the two first claws to move up, and the driving shaft first enters the first claw, so that the driven gear is meshed with the driven rack, and the second claw is separated from the driving shaft. When the lifting frame moves down to the rocking plate, the lifting frame drives the driving shaft to move downward, and the second bracket is inserted into the lifting frame, and the driving shaft first enters the second claw, and the first claw is separated from the driving shaft, so that the driven gear is disengaged from the driven rack. At this time, the elastic member pushes the slide plate to move and reset through the push block, so that the first elastic rope and the second elastic rope return to a mutually perpendicular state again, thereby making the limiting effect of the upper elastic net better.

[0018] Preferably, a first accommodating hole is formed in the first claw, and a first guide wall is provided on the first claw to facilitate the drive shaft to enter the first accommodating hole. A second accommodating hole is formed in the second claw, and a second guide wall is provided on the second claw to facilitate the drive shaft to enter the second accommodating hole.

[0019] By adopting the above technical solution, the first guide wall and the second guide wall are used to facilitate the drive shaft to enter the first claw and the second claw, so that the drive shaft can switch between the first claw and the second claw more smoothly.

[0020] Preferably, the side of the lifting frame diagonal close to the two slides is area A, which is used to place conical flasks, and the side of the lifting frame diagonal away from the two slides is area B, which is used to place test tubes.

[0021] By adopting the above technical solution, area A of the lifting frame is the side close to the slide, so the inclination and deflection of the first elastic rope and the second elastic rope in area A are larger, which is convenient for placing the conical flask. Area B of the lifting frame is the side away from the slide, so the inclination and deflection of the first elastic rope and the second elastic rope in area B are smaller, which is convenient for placing the test tube.

[0022] Preferably, a limit block is fixedly provided on the top of the column, a first magnetic part is provided on the bottom wall of the limit block and the top wall of the rocking plate, and a second magnetic part is provided on the top and bottom walls of the lifting frame, and the first magnetic part and the second magnetic part attract each other.

[0023] By adopting the above technical solution, when the lifting frame moves upward, the limit block limits the moving position of the lifting frame, and the first magnetic part at the bottom of the limit block and the second magnetic part at the top of the lifting frame attract each other, so that the lifting frame can be suspended in mid-air, thereby facilitating the placement of the conical bottle. When the lifting frame descends to the rocking plate, the first magnetic part at the top of the rocking plate and the second magnetic part at the bottom of the lifting frame attract each other, so that the lifting frame is not easy to move during the shaking of the rocking plate.

[0024] In summary, this application includes at least one of the following beneficial technical effects:

[0025] 1. When using the lifting frame to limit the position of the conical flask, first slide the lifting frame upwards, which drives the upper elastic net to slide upwards, then place the conical flask on the lower elastic net of the rocking plate, and finally slide the lifting frame downwards, which drives the upper elastic net to slide downwards. The top of the conical flask passes through the limiting opening of the upper elastic net, making it easier to limit the position of the conical flask;

[0026] 2. With the help of the drive assembly, when the lifting frame drives the upper elastic net downward, if the first elastic cord or the second elastic cord is located directly above the mouth of the conical flask, the first elastic cord or the second elastic cord will press down on the mouth of the conical flask, preventing the mouth of the conical flask from entering the restricted opening of the upper elastic net. When the lifting frame moves downward, the two drive racks drive the two slides within the lifting frame through the two drive assemblies. The two slides respectively drive one end of the first elastic cord and the second elastic cord to move, causing the first elastic cord and the second elastic cord pressing down on the mouth of the conical flask to deflect, thereby facilitating the mouth of the conical flask to enter the restricted opening of the upper elastic net.

[0027] 3. Through the first magnetic part and the second magnetic part, when the lifting frame moves upward, the limit block limits the moving position of the lifting frame, and the first magnetic part at the bottom of the limit block and the second magnetic part at the top of the lifting frame attract each other, so that the lifting frame can be suspended in mid-air, thereby facilitating the placement of the conical flask. When the lifting frame descends to the rocking plate, the first magnetic part at the top of the rocking plate and the second magnetic part at the bottom of the lifting frame attract each other, so that the lifting frame is not easy to move during the shaking of the rocking plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 Schematic diagram of the overall structure of the cell oscillation culture device in Example 1 of the present application;

[0029] Figure 2 This is a schematic diagram of the structure of the cell oscillation culture device in Example 1 of the present application, highlighting the interior of the chassis;

[0030] Figure 3 This is a schematic diagram of a portion of the structure of the cell oscillation culture device in Example 1 of the present application;

[0031] Figure 4 This is a partial structural diagram of the cell oscillation culture device in Example 2 of the present application;

[0032] Figure 5 This is a partial structural cross-sectional view of the cell oscillation culture device in Example 2 of the present application;

[0033] Figure 6 This is an exploded cross-sectional view of a portion of the structure of the cell oscillation culture device in Example 2 of the present application;

[0034] Figure 7 For this application Figure 6 The enlarged schematic diagram of point C in the middle;

[0035] Figure 8 For this application Figure 6 The enlarged schematic diagram of point D in the middle;

[0036] Figure 9 For this application Figure 6 Enlarged schematic diagram at point E in the middle.

[0037] Figure 1: Chassis; 2: Rocker plate; 3: Cover; 4: Lower elastic net; 5: Column; 6: Lifting frame; 7: Upper elastic net; 71: First elastic rope; 72: Second elastic rope; 8: Slide plate; 9: Drive assembly; 91: Drive shaft; 92: Drive gear; 93: Driven rack; 94: Anti-return component; 941: Ratchet; 942: Elastic reset member; 95: Driven gear; 10: Drive rack; 11: Ratchet Slot; 12. Push block; 13. Elastic member; 14. First bracket; 15. First clamping claw; 16. Second bracket; 17. Second clamping claw; 18. First accommodating hole; 19. First guide wall; 20. Second accommodating hole; 21. Second guide wall; 22. Area A; 23. Area B; 24. Limit block; 25. First magnetic member; 26. Second magnetic member; 27. Lifting slot; 28. Guide rod; 29. Guide seat; 30. Slot. DETAILED DESCRIPTION

[0038] The following is combined with Figures 1-9 This application is described in further detail.

[0039] Example 1:

[0040] The embodiments of the present application disclose a cell oscillation culture device.

[0041] Reference Figure 1 and Figure 2A cell oscillation culture device includes a chassis 1, a cover 3 is rotatably installed on the top of the chassis 1, a driving mechanism is installed in the chassis 1, a rocking plate 2 is installed in the chassis 1, and the rocking plate 2 is installed on the driving end of the driving mechanism. The driving mechanism can drive the rocking plate 2 to shake on a horizontal plane. The driving mechanism is an existing technology and will not be described in detail here.

[0042] Reference Figure 2 and Figure 3 A lower elastic net 4 is installed inside the rocking board 2. The lower elastic net 4 is composed of multiple elastic ropes. Vertical posts 5 are fixedly mounted at the four corners of the top wall of the rocking board 2. A lifting frame 6 is vertically slidably mounted on the four posts 5. An upper elastic net 7 is installed inside the lifting frame 6. The upper elastic net 7 is located directly above the lower elastic net 4. Both the rocking board 2 and the lifting frame 6 are rectangular.

[0043] To position the Erlenmeyer flask, first slide the lifting frame 6 upward, causing it to simultaneously raise the upper elastic mesh 7, thereby creating sufficient space between the upper elastic mesh 7 and the lower elastic mesh 4. Next, place the Erlenmeyer flask steadily on the lower elastic mesh 4 of the rocking plate 2, ensuring its bottom is secure. Then, slide the lifting frame 6 downward, causing the upper elastic mesh 7 to descend with it, until the top of the Erlenmeyer flask passes through the position-limiting opening of the upper elastic mesh 7.

[0044] The cooperation between the upper elastic mesh 7 and the lower elastic mesh 4 secures the conical flask between the two layers of mesh, preventing it from shifting or tipping during shaking while ensuring the stability and safety of the experiment. Furthermore, this positioning method prevents the bottom of the conical flask from passing through the positioning opening, thus facilitating the positioning of the conical flask.

[0045] A limit block 24 is fixedly installed on the top of each column 5, and a first magnetic part 25 is fixedly embedded and installed on the bottom wall of the four limit blocks 24 and the four corners of the top wall of the rocking plate 2. A second magnetic part 26 is fixedly embedded and installed on the top wall and bottom wall at the four corners of the lifting frame 6. The first magnetic part 25 and the second magnetic part 26 attract each other. In this application, the first magnetic part 25 and the second magnetic part 26 can both be selected as magnets.

[0046] As the lifting frame 6 moves upward, the stopper 24 limits its range of movement, preventing it from rising excessively. Simultaneously, the first magnetic member 25 at the bottom of the stopper 24 and the second magnetic member 26 at the top of the lifting frame 6 attract each other through magnetic force, allowing the lifting frame 6 to remain stably suspended in mid-air, making it easier to place a conical flask.

[0047] When the lifting frame 6 descends onto the rocking plate 2, the first magnetic member 25 at the top of the rocking plate 2 and the second magnetic member 26 at the bottom of the lifting frame 6 are magnetically attracted to each other again, forming a secure connection. This magnetic attraction design not only ensures that the lifting frame 6 is not easily displaced during the rocking plate 2, but also enhances the stability of the overall structure, making experimental operations more convenient and safer.

[0048] The cell oscillation culture device of the present embodiment is implemented as follows: When culturing cells, the Erlenmeyer flask is placed first, followed by the test tube. To position the Erlenmeyer flask, the lifting frame 6 is first slid upward, causing the upper elastic mesh 7 to rise synchronously, thereby creating sufficient space between the upper elastic mesh 7 and the lower elastic mesh 4. Next, the Erlenmeyer flask is placed steadily on the lower elastic mesh 4 of the rocking plate 2, ensuring its bottom is secure. The lifting frame 6 is then slid downward, causing the upper elastic mesh 7 to descend, until the top of the Erlenmeyer flask passes through the retaining opening of the upper elastic mesh 7. This retaining mechanism prevents the bottom of the Erlenmeyer flask from passing through the retaining opening, facilitating the positioning of the Erlenmeyer flask. After the Erlenmeyer flask is positioned, the test tube is inserted into the retaining opening between the upper elastic mesh 7 and the lower elastic mesh 4, thereby securing the test tube. A drive mechanism is used to rock the rocking plate 2, which in turn rock the Erlenmeyer flask and test tube, thereby enabling cell culture.

[0049] Example 2:

[0050] Reference Figure 4 This embodiment differs from the first embodiment in that slide plates 8 are mounted horizontally and slideably on two adjacent inner side walls of the lifting frame 6, and push blocks 12 are mounted horizontally and slideably on two adjacent top walls of the lifting frame 6. The two push blocks 12 are fixedly connected to the two slide plates 8. A drive rack 10 is fixedly mounted on the side walls of the lifting frame 6, on the side away from the two slide plates 8.

[0051] The upper elastic net 7 is composed of multiple first elastic ropes 71 and second elastic ropes 72, wherein the two ends of the first elastic ropes 71 are respectively fixed to the inner wall of the lifting frame 6 and one of the slides 8, and the multiple first elastic ropes 71 are arranged parallel to each other; the two ends of the second elastic ropes 72 are respectively fixed to the inner wall of the lifting frame 6 and the other slide 8, and the multiple second elastic ropes 72 are also arranged parallel to each other, and the first elastic ropes 71 and the second elastic ropes 72 are vertically cross-distributed on the horizontal plane.

[0052] Two drive assemblies 9 are installed inside the lifting frame 6. These two drive assemblies 9 are located on the side of the two slides 8 that are away from each other. The two drive assemblies 9 drive the drive racks 10 to the slides 8. When the lifting frame 6 moves downward, the drive racks 10 drive the slides 8 to move horizontally within the lifting frame 6 through the drive assemblies 9, thereby adjusting the position of the upper elastic net 7.

[0053] When the lifting frame 6 drives the upper elastic net 7 to descend, if the first elastic rope 71 or the second elastic rope 72 is located just above the mouth of the conical flask, the first elastic rope 71 or the second elastic rope 72 may press down on the mouth of the conical flask, causing the mouth of the conical flask to be unable to smoothly enter the limit opening of the upper elastic net 7.

[0054] To address this issue, as the lifting frame 6 continues to descend, the two drive racks 10, via the drive assembly 9, respectively drive the two slides 8 within the lifting frame 6, moving away from each other. The movement of the slides 8 deflects one end of the first and second elastic cords 71, 72, causing the elastic cords, originally pressed against the mouth of the conical flask, to move away from the mouth. This design allows the mouth of the conical flask to smoothly enter the retaining opening of the upper elastic net 7, ensuring that the conical flask is securely held in place.

[0055] In the diagonal of the lifting frame 6 at both ends of which are located at the two driving racks 10, the upper elastic net 7 is located on the side of the diagonal close to the two slides 8 as area A 22, which is used to place conical flasks; the upper elastic net 7 is located on the side of the diagonal away from the two slides 8 as area B 23, which is used to place test tubes.

[0056] Because area A 22 is close to slide 8, the movement of slide 8 has a more significant impact on the first and second elastic cords 71, 72 within area A 22, causing the elastic cords within area A 22 to tilt more sharply. This design facilitates the placement and positioning of the Erlenmeyer flask, ensuring it can securely pass through the retaining opening of the upper elastic net 7. After the Erlenmeyer flask is positioned, the test tube can be placed in area B 23.

[0057] Reference Figure 5 、 Figure 6 、 Figure 7 and Figure 8 Specifically, the drive assembly 9 includes a drive shaft 91, a drive gear 92, a driven gear 95, a driven rack 93, and a check member 94. First brackets 14 are fixedly mounted on both sides of each drive shaft 91 within the lifting frame 6. First claws 15 are fixedly mounted on the top of the first brackets 14. The ends of the drive shaft 91 are detachably and rotatably mounted on the two first claws 15. Four lifting slots 27 are defined within the lifting frame 6. The ends of each drive shaft 91 are inserted into two of the lifting slots 27, respectively, and the drive shaft 91 can be raised and lowered within the lifting slots 27.

[0058] The driving gear 92 is rotatably mounted on the driving shaft 91. The anti-return component 94 is mounted at the connection between the driving shaft 91 and the driving gear 92 to control the linkage relationship between the driving shaft 91 and the driving gear 92. Two driven racks 93 and two driven gears 95 are installed. The two driven racks 93 are fixedly mounted at the ends of the slide 8 at intervals. The two driven gears 95 are fixedly mounted at both ends of the driving shaft 91, and the two driven gears 95 are respectively engaged with the two driven racks 93.

[0059] When the lifting frame 6 moves upward, the driving rack 10 drives the driving gear 92 to rotate in the forward direction. Due to the effect of the anti-return component 94, the driving gear 92 rotates idly on the driving shaft 91 and does not drive the driving shaft 91 to rotate, so the slide plate 8 remains stationary and does not move.

[0060] As the lifting frame 6 descends, the drive rack 10 drives the drive gear 92 in the opposite direction. At this point, the anti-return component 94 locks the drive gear 92 and the drive shaft 91, allowing the drive gear 92 to rotate synchronously with the drive shaft 91. The rotation of the drive shaft 91 further drives the two driven gears 95. These two driven gears 95, meshing with the two driven racks 93, drive the slide 8 to slide horizontally within the lifting frame 6, causing the two slides 8 to move away from each other within the lifting frame 6.

[0061] The movement of the two slides 8 causes one end of the first and second elastic cords 71 and 72 to deflect, thereby moving the first and second elastic cords 71 and 72, which might have been pressed against the mouth of the conical flask, away from the mouth of the flask. This linkage mechanism ensures that the mouth of the conical flask can smoothly enter the restricted opening of the upper elastic net 7, preventing the elastic cords from interfering with the mouth of the flask.

[0062] Specifically, the anti-return component 94 includes two pawls 941 and an elastic return member 942. The two pawls 941 are rotatably mounted on the outer wall of the drive shaft 91 at equal intervals along the circumference of the drive shaft 91. The two elastic return members 942 are installed at the rotational connection between the pawls 941 and the drive shaft 91 to provide a return force. In this application, the elastic return members 942 can be used as torsion springs. A ratchet groove 11 is defined in the side wall of the drive gear 92, and the two pawls 941 are located in the ratchet groove 11, forming a ratchet mechanism.

[0063] When the lifting frame 6 ascends, the drive rack 10 drives the drive gear 92 to rotate in the forward direction. The rotation of the drive gear 92 passes through multiple ratchets 941. Because the ratchets 941 are able to rotate freely under the action of the elastic return members 942, the rotation of the drive gear 92 is not hindered. The ratchets 941 slide within the ratchet grooves 11, allowing the drive gear 92 to idle on the drive shaft 91 without driving the drive shaft 91 to rotate. At this time, the slide plate 8 remains stationary and does not move.

[0064] When the lifting frame 6 descends, the drive rack 10 drives the drive gear 92 to rotate in the opposite direction, and the pawl 941 is locked in the ratchet groove 11, preventing it from rotating freely. At this point, the meshing action between the pawl 941 and the ratchet groove 11 locks the drive gear 92 and the drive shaft 91 together. The rotation of the drive gear 92 directly drives the synchronous rotation of the drive shaft 91. The rotation of the drive shaft 91 further drives the driven gear 95, which, through its meshing with the driven rack 93, drives the slide plate 8 to slide horizontally within the lifting frame 6.

[0065] Reference Figure 4 and Figure 5 A guide rod 28 is fixedly mounted on the end wall of the push block 12 away from the drive rack 10. Both guide rods 28 are sleeved with an elastic member 13, which is located on the side of the push block 12 near the drive assembly 9. A guide seat 29 is fixedly mounted on the top wall of the lifting frame 6. The end of the guide rod 28 away from the push block 12 slides through the guide seat 29, and the two ends of the elastic member 13 respectively abut the push block 12 and the guide seat 29. The two elastic members 13 abut the two push blocks 12 and are used to drive the two slides 8 to move and reset. In this application, the elastic members 13 can be springs.

[0066] Reference Figure 6 、 Figure 7 、 Figure 8 and Figure 9 Four second brackets 16 are fixedly mounted on the top wall of the rocker plate 2. Four slots 30 are defined within the lift frame 6 and communicate with the lift slots 27. The top ends of the four second brackets 16 are inserted into the slots 30 of the lift frame 6 and are located at the ends of the two drive shafts 91. Second claws 17 are mounted on the top ends of the second brackets 16. The ends of the drive shafts 91 are also detachably and rotatably mounted within the second claws 17.

[0067] A first receiving hole 18 is formed in the first clamping jaw 15, and the drive shaft 91 is rotatably mounted in the first receiving hole 18. Two first guide walls 19 are formed on the first clamping jaw 15 above the first receiving hole 18. The two first guide walls 19 are used to guide the drive shaft 91 smoothly into the first receiving hole 18. Similarly, a second receiving hole 20 is formed in the second clamping jaw 17, and a second guide wall 21 is formed on the second clamping jaw 17 to guide the drive shaft 91 smoothly into the second receiving hole 20.

[0068] When the lifting frame 6 is located on the rocking plate 2 , the driving shaft 91 is rotatably mounted in the second claw 17 , and at this time, the driven gear 95 is disengaged from the driven rack 93 .

[0069] As the lifting frame 6 rises from the rocker plate 2, it drives the two first claws 15 upward synchronously. The drive shaft 91 first passes through the first guide wall 19 and enters the first claw 15, causing the driven gear 95 to engage with the driven rack 93. The drive shaft 91 then separates from the second claw 17. At this point, the connection between the drive shaft 91 and the first claw 15 ensures the proper functioning of the drive assembly 9. Driven by the drive rack 10 and the drive assembly 9, the slide 8 moves, adjusting the position of the upper elastic net 7. Simultaneously, as the slide 8 slides within the lifting frame 6, it drives the push block 12 to compress the elastic member 13, causing it to contract and deform, storing elastic potential energy.

[0070] When the lifting frame 6 descends onto the rocking plate 2, it drives the drive shaft 91 downward. The second bracket 16 is inserted into the lifting frame 6, and the drive shaft 91 first passes through the second guide wall 21 and enters the second claw 17. The second claw 17 then drives the drive shaft 91 upward and disengages from the first claw 15. At this point, the driven gear 95 disengages from the driven rack 93, and the drive assembly 9 stops operating. The elastic member 13 releases its elastic potential energy and, through the push block 12, pushes the slide plate 8 back to its original position, returning the first and second elastic cords 71, 72 to their perpendicular position. This restores the original position of the upper elastic net 7 and ensures a more effective position control.

[0071] The operating principle of this second embodiment is as follows: As the lifting frame 6 continues to descend, the drive rack 10 drives the drive gear 92 to rotate in the opposite direction. At this point, the pawl 941 is locked in the ratchet groove 11, preventing it from rotating freely. The meshing action between the pawl 941 and the ratchet groove 11 locks the drive gear 92 and the drive shaft 91 together. The rotation of the drive gear 92 directly drives the synchronous rotation of the drive shaft 91. The rotation of the drive shaft 91 further drives the two driven gears 95 to rotate. These driven gears 95, through engagement with the two driven racks 93, drive the slide 8 to slide horizontally within the lifting frame 6, causing the two slides 8 to move away from each other within the lifting frame 6. The movement of the slide 8 deflects one end of the first and second elastic cords 71 and 72, causing the elastic cords originally pressed against the mouth of the conical flask to move away from the mouth. This allows the mouth of the conical flask to smoothly enter the retaining opening of the upper elastic net 7, ensuring that the conical flask is securely positioned.

[0072] The above are merely optional embodiments of the present disclosure and are not intended to limit the present disclosure. Those skilled in the art will readily appreciate that the present disclosure may be modified and varied in various ways. Any modifications, equivalent substitutions, improvements, and the like made within the spirit and principles of the present disclosure shall be included within the scope of protection of the present disclosure.

Claims

1. A cell oscillation culture device, characterized in that: The invention comprises a chassis (1), a rocking plate (2) is arranged in the chassis (1), a driving mechanism is arranged in the chassis (1), a driving end of the driving mechanism is connected to the rocking plate (2) and drives the rocking plate (2) to rock back and forth, a lower elastic net (4) is arranged in the rocking plate (2), a plurality of columns (5) are arranged on the rocking plate (2), a lifting frame (6) is arranged on the plurality of columns (5) to lift and slide, an upper elastic net (7) is arranged in the lifting frame (6), and the upper elastic net (7) is located directly above the lower elastic net (4).

2. A cell oscillation culture device according to claim 1, characterized in that: Slide plates (8) are provided on the two adjacent inner side walls of the lifting frame (6) for sliding in the horizontal direction. The upper elastic net (7) includes a plurality of first elastic ropes (71) and a second elastic rope (72). The two ends of the first elastic rope (71) are fixedly connected to the inner wall of the lifting frame (6) and a slide plate (8) respectively. The plurality of first elastic ropes (71) are parallel to each other. The two ends of the second elastic rope (72) are fixedly connected to the inner wall of the lifting frame (6) and another slide plate (8) respectively. The plurality of second elastic ropes (72) are parallel to each other, and the first elastic rope (71) and the second elastic rope (72) are perpendicular to each other. A driving rack (10) is provided on the two uprights (5). Two driving assemblies (9) are provided in the lifting frame (6). The driving assembly (9) is connected to the driving rack (10) and the slide plate (8). When the lifting frame (6) moves downward, the driving rack (10) drives the slide plate (8) to move in the lifting frame (6) through the driving assembly (9).

3. A cell oscillation culture device according to claim 2, characterized in that: The driving assembly (9) comprises a driving shaft (91), a driving gear (92), a driven gear (95), a driven rack (93) and a backstop component (94). The driving shaft (91) is rotatably arranged in the lifting frame (6). The driving gear (92) is rotatably arranged on the driving shaft (91) and meshes with the driving rack (10). The driven rack (93) is fixedly arranged at the end of the slide plate (8). The driven gear (95) is fixedly arranged on the driving shaft (91) and meshes with the driven rack (93). The backstop component (94) is arranged at the connection between the driving shaft (91) and the driving gear (92). When the lifting frame (6) moves upward, the driving rack (10) drives the driving gear (92) to rotate on the driving shaft (91). When the lifting frame (6) moves downward, the backstop component (94) locks the driving gear (92) and the driving shaft (91), and the driving gear (92) drives the driving shaft (91) to rotate.

4. A cell oscillation culture device according to claim 3, characterized in that: The anti-return component (94) includes a plurality of ratchet pawls (941) and elastic reset members (942), wherein the plurality of ratchet pawls (941) are rotatably arranged on the outer side wall of the drive shaft (91), and the plurality of elastic reset members (942) are arranged at the rotation connection between the plurality of ratchet pawls (941) and the drive shaft (91), and a ratchet groove (11) is opened on the side wall of the drive gear (92), and the plurality of ratchet pawls (941) are located in the ratchet groove (11).

5. A cell oscillation culture device according to claim 3, characterized in that: A push block (12) is provided on the side wall of the slide plate (8), and an elastic member (13) is provided in the lifting frame (6). The elastic member (13) abuts against the push block (12) and is used to drive the slide plate (8) to move and reset. A first bracket (14) is fixedly provided at both ends of the two driving shafts (91) in the lifting frame (6), and a first claw (15) is provided at the top end of the first bracket (14). The two ends of the driving shaft (91) are detachably rotatable and are set in the two first claws (15). Four second brackets (16) are fixedly provided on the top wall of the rocking plate (2), and the top end of the second bracket (16) is inserted into the lifting frame (6). The second bracket ( The top of the lifting frame (6) is provided with a second claw (17), and the two ends of the driving shaft (91) are detachably rotatable and arranged in the second claw (17). When the lifting frame (6) moves upward from the rocking plate (2), the lifting frame (6) drives the two first claws (15) to move upward, the driving shaft (91) first enters the first claw (15), and the second claw (17) is separated from the driving shaft (91). When the lifting frame (6) moves down to the rocking plate (2), the lifting frame (6) drives the driving shaft (91) to move downward, the second bracket (16) is inserted into the lifting frame (6), the driving shaft (91) first enters the second claw (17), and the first claw (15) is separated from the driving shaft (91).

6. A cell oscillation culture device according to claim 5, characterized in that: A first receiving hole (18) is formed in the first clamping claw (15), and a first guide wall (19) is provided on the first clamping claw (15) for facilitating the drive shaft (91) to enter the first receiving hole (18). A second receiving hole (20) is formed in the second clamping claw (17), and a second guide wall (21) is provided on the second clamping claw (17) for facilitating the drive shaft (91) to enter the second receiving hole (20).

7. A cell oscillation culture device according to claim 3, characterized in that: The side of the lifting frame (6) diagonally close to the two slides (8) is area A (22), and area A (22) is used to place conical flasks. The side of the lifting frame (6) diagonally away from the two slides (8) is area B (23), and area B (23) is used to place test tubes.

8. The cell oscillation culture device according to claim 1, characterized in that: A limit block (24) is fixedly provided on the top of the column (5), a first magnetic member (25) is provided on the bottom wall of the limit block (24) and the top wall of the rocking plate (2), and a second magnetic member (26) is provided on the top wall and the bottom wall of the lifting frame (6), and the first magnetic member (25) and the second magnetic member (26) attract each other.

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