A plant extract telomerase factor repair cell anti-aging and sleep-inducing compound preparation

Abstract

The present invention belongs to the field of anti-aging and sleep-aiding compound preparations, and particularly relates to a plant extract telomerase factor repair cell anti-aging and sleep-aiding compound preparation. The plant component containing telomerase factor activation protein obtained by crushing and extraction and the Monascus extract are mixed with a carrier solution with a high specific surface area, and subjected to freeze nano-spray drying. The obtained repair cell extract preparation can efficiently repair cells; the anti-aging mixture is mixed with the sleep-aiding powder to form granules, and the mixture is sprayed to promote transdermal absorption. The anti-aging and sleep-aiding composition obtained by air-drying can be slowly released in the gastrointestinal tract to improve the absorption efficiency; the stabilizing viscous component cooperates with the anti-aging and sleep-aiding composition to slow down the release rate of the drug and help the effective ingredients in the repair cell anti-aging and sleep-aiding preparation to dissolve better. The prepared plant extract telomerase factor repair cell anti-aging and sleep-aiding compound preparation has a stable effect, does not burden the liver and kidneys with long-term use, and can maintain the effect for a long time.

Description

Technical Field

[0001] The present invention belongs to the field of anti-aging and sleep-aiding compound preparations, and specifically refers to a plant extract telomerase factor repair cell anti-aging and sleep-aiding compound preparation. Background Art

[0002] Telomerase activating protein (HTP) activates telomerase activity, extending telomere length, and helping to restore human DNA. This in turn promotes cell activity, slowing the renewal and aging process of human cells, thereby delaying human aging. By improving telomerase activity, HTP can help restore the pituitary gland to a youthful state, thereby promoting the normal functioning of nervous, endocrine, and metabolic systems, effectively delaying the aging process of joints and organs.

[0003] Telomerase activating protein can enhance myocardial metabolism, dilate blood vessels, improve microcirculation, reduce vascular resistance, increase blood flow, and effectively prevent conditions such as myocardial ischemia, hypertension, and arteriosclerosis. Furthermore, this protein helps restore kidney function, increase the production of antioxidant molecules in the blood, regulate endocrine function, and balance hormones. Telomerase activating protein can also effectively remove various toxins from the blood, activate blood cell activity, increase blood oxygen content and purity, and provide sufficient nutrition to cells throughout the body through blood circulation, ensuring the normal function of organs. This protein can also effectively restore the body's lipid metabolism balance and increase the metabolism of accumulated fat.

[0004] At present, the existing preparation technology of repair cell anti-aging sleep-aiding compound preparations has the following problems: First, the existing repair cell anti-aging sleep-aiding preparations have slow efficacy and unstable effects; second, long-term use of existing repair cell anti-aging sleep-aiding preparations will cause a burden on the liver and kidneys; third, the effects of existing repair cell anti-aging sleep-aiding preparations are not long-lasting after use, and long-term use requires continuously increasing the dosage. Summary of the Invention

[0005] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a plant extract telomerase factor repair cell anti-aging sleep-aiding compound preparation. In order to solve the problems of the existing cell repair anti-aging sleep-aiding compound preparation having slow efficacy, unstable effect, burden on the liver and kidneys with long-term use, and inability to exert long-term effects, the present invention uses a repair cell extract preparation, an anti-aging sleep-aiding composition, and a stabilizing viscous component to mix. The prepared plant extract telomerase factor repair cell anti-aging sleep-aiding compound preparation has stable efficacy, does not burden the liver and kidneys with long-term use, and can maintain the effect for a long time.

[0006] In order to achieve the above-mentioned object, the technical solution adopted by the present invention is as follows: the present invention proposes a plant extract telomerase factor repair cell anti-aging sleep-inducing composite preparation, the raw materials for preparing the plant extract telomerase factor repair cell anti-aging sleep-inducing composite preparation specifically include the following components in parts by weight:

[0007] 22-26 parts of repair cell extract preparation, 13-17 parts of anti-aging and sleep-aiding composition, 2.2-2.8 parts of hydroxypropyl cellulose, 0.6-0.9 parts of copovidone, 1.1-1.4 parts of tartaric acid, and 1.2-1.5 parts of propyl gallate.

[0008] Preferably, the raw materials for preparing the repair cell extract preparation include the following components in parts by weight: 6-8 parts of strawberry seeds, 5-7 parts of grape peels, 2-3 parts of tomato leaves, 4-8 parts of wild ginseng, 3-7 parts of pomegranate seeds, 1.1-1.3 parts of Monascus, 2.5-3.5 parts of bile acid, 3-4 parts of L-lysine diisocyanate, and 1.5-1.8 parts of glycerol.

[0009] Preferably, the raw materials for preparing the anti-aging and sleep-aiding composition include the following components in parts by weight: 4-6 parts of fish oil, 2-4 parts of pumpkin seeds, 3-6 parts of ginkgo leaves, 4-5 parts of valerian, 2.5-3 parts of lemon balm, 2-4 parts of walnuts, 3-4.5 parts of lily, 2.6-3.2 parts of sucrose laurate, 2.5-3.2 parts of corn protein, and 1.1-1.3 parts of glutaraldehyde.

[0010] Preferably, the preparation method of the repair cell extract preparation specifically comprises the following steps:

[0011] S1. Place strawberry seeds, grape peels, tomato leaves, wild ginseng, and pomegranate seeds into a 1.5-1.8 kW grinder at a grinding temperature of 23-28° C. for 15-20 min at a grinding speed of 3500-4500 r / min. After grinding, place the mixture with ultrapure water into a 3.5-3.8 kW fully automatic extractor at an extraction pressure of 12 MPa, an extraction temperature of 50° C., and an extraction time of 30-40 min to obtain a plant component containing telomerase factor activating protein;

[0012] S2. Inoculate Monascus into a 5% sterile glucose solution, place the solution in a constant temperature shaking incubator with a power of 2.2-2.6 kW, set the constant temperature shaking incubation temperature to 33°C, the constant temperature shaking incubation time to 24-30 h, and the constant temperature shaking incubation speed to 270 r / min. After constant temperature shaking incubation, place the solution in an ultrasonic cell disruptor with a power of 1.2-1.5 kW, set the ultrasonic disruption frequency to 20 kHz, set the ultrasonic disruption temperature to 0°C, and set the ultrasonic disruption time to 10-13 min. After disruption, filter the solution through a 0.22 mm sterile filter membrane to obtain a Monascus extract.

[0013] S3, placing bile acid, L-lysine diisocyanate, glycerol, and dimethyl sulfoxide in a 2.4 kW reactor, reacting at a temperature of 100° C., a reaction rate of 300 r / min, and a reaction time of 8 h, mixing and performing a cross-linking reaction to obtain a carrier solution;

[0014] S4. Place the carrier solution prepared in S3, the plant component containing telomerase factor activating protein prepared in S1, and the Monascus extract prepared in S2 into a stirring tank with a power of 1.5 kW, stirring at a temperature of 35°C, a stirring time of 1 h, and a stirring speed of 400 r / min for sufficient adsorption to obtain a repair cell solution;

[0015] S5. Place the repair cell solution prepared in S4 into a vacuum freeze-nano spray dryer with a power of 2.3 kW, a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 2.5 h for freeze-spray drying to obtain a repair cell extract preparation.

[0016] Furthermore, in S1, the material-liquid ratio of the strawberry seeds to ultrapure water is 1:2 g / mL.

[0017] Furthermore, in S2, the Monascus was purchased from the China General Microbiological Culture Collection Center with a collection number of CGMCC3.7036, and the solid-liquid ratio of the Monascus to 5% sterile glucose solution was 1:100 g / mL.

[0018] Furthermore, in S3, the mass fraction of bile acid in dimethyl sulfoxide is 3%.

[0019] Preferably, the preparation method of the anti-aging and sleep-aiding composition specifically comprises the following steps:

[0020] L1. Place fish oil, pumpkin seeds, and ginkgo leaves into a refiner with a power of 1.5-1.8 kW, at a refining speed of 3000 r / min, a refining temperature of 28-33° C., and a refining time of 10-15 min to obtain an anti-aging mixture;

[0021] L2. Place valerian, lemon balm, walnut, and lily in a 5.5-7.5 kW vacuum freeze dryer at a vacuum degree of 60-80 Pa and a freezing temperature of -20°C for 2-3 hours. Then, warm the freeze dryer to 2-4°C and dry for 1.2-1.5 hours. Then, pulverize the freeze dryer using a 1.6-2.2 kW powdering machine at a temperature of 25-28°C for 20-25 minutes to obtain a sleep-inducing powder.

[0022] L3. Place sucrose laurate, zein, glutaraldehyde, and 70% ethanol solution in a 2.4 kW reactor, react at a temperature of 40-50° C., a reaction rate of 300 r / min, and a reaction time of 6 h, and mix and react to obtain a mixture that promotes transdermal absorption;

[0023] L4, the anti-aging mixture prepared in L1 and the sleep-aiding powder prepared in L2 are placed in a stirrer with a power of 1.2-1.4 kW, the stirring temperature is 23-28° C., the stirring time is 20-30 min, the stirring speed is 300 r / min, and after mixing, the mixture is placed in a granulator with a power of 2.1-2.4 kW, a granulation temperature of 28-32° C., a granulation time of 30-45 min, and a granulation diameter of 1.2-1.6 mm, and granulated to obtain the anti-aging and sleep-aiding component;

[0024] L5. Use a sprayer with a power of 1.6-2.3 kW to evenly spray the mixture prepared in L3 on the surface of the anti-aging and sleep-aiding component prepared in L4. The mixture is placed at a temperature of 25-30°C for 2-2.5 hours at a temperature of 25-28°C and air-dried to obtain the anti-aging and sleep-aiding composition.

[0025] Furthermore, in L3, the mass fraction of the sucrose laurate in the 70% ethanol solution is 5%.

[0026] Preferably, the preparation method of the plant extract telomerase factor cell repair anti-aging and sleep-inducing composite preparation specifically comprises the following steps:

[0027] Step 1: Place hydroxypropyl cellulose, copovidone, tartaric acid, propyl gallate, and 70% ethanol solution in a stirrer with a power of 1.2-1.4 kW, stir at a temperature of 23-28° C., stir for 10-15 minutes, and stir at a speed of 500 r / min to obtain a stabilizing viscous component;

[0028] Step 2: Place the repair cell extract preparation and the anti-aging sleep-aiding composition into a stirring tank with a power of 1.5 kW, stir at a temperature of 20° C., a stirring speed of 300 r / min, and a stirring time of 18 min, and stir and mix to obtain the repair cell anti-aging sleep-aiding preparation;

[0029] Step 3: Place the stabilizing viscous component prepared in step 1 into the cell repair, anti-aging, and sleep-aiding preparation prepared in step 2, with a stirring temperature of 20°C, a stirring speed of 300 r / min, and a stirring time of 20 min. Mix thoroughly, place in a tablet press with a power of 1.8-2.2 kW, a tableting temperature of 20-25°C, a tableting time of 18-22 min, and a tableting diameter of 8-11 mm. After tableting, a plant extract telomerase factor cell repair, anti-aging, and sleep-aiding composite preparation is obtained.

[0030] Furthermore, in step 1, the mass fraction of the hydroxypropyl cellulose in the 70% ethanol solution is 2%.

[0031] The beneficial effects achieved by the present invention are as follows:

[0032] The present invention uses strawberry seeds, grape peels, tomato leaves, wild ginseng, and pomegranate seeds for crushing and extraction to obtain a plant component containing telomerase factor activation protein that is rich in telomerase factor activation protein. Telomerase factor plays a key role in the cell repair process and can activate telomerase in cells, extend the length of cell telomeres, thereby delaying cell aging and enhancing the self-repair ability of cells. The saponin components in wild ginseng also have significant anti-fatigue and immunity-enhancing effects, and can provide rich nutritional support for cell repair. Monascus extract has high cell compatibility and contains antioxidant components such as phenolic substances and short-chain fatty acids, which can effectively scavenge free radicals in cells and inhibit inflammation. The expression of metalloproteinases induced by telomerase is inhibited. By inhibiting the activity of these enzymes, Monascus extract can protect the integrity of the extracellular matrix and provide a stable microenvironment for cells, thereby promoting cell repair. After bile acid is mixed with L-lysine diisocyanate and glycerol, the isocyanate group of L-lysine diisocyanate reacts with the hydroxyl groups in bile acid and glycerol to generate carbamate bonds, forming multiple cross-linking points, so that the molecules are interconnected to form a three-dimensional network structure with a high specific surface area. The plant components containing telomerase factor activating protein and Monascus extract are fully adsorbed and freeze-nano spray-dried to form a porous solid to obtain the repair cell extract. The preparation can efficiently repair cells; after grinding fish oil, pumpkin seeds, and ginkgo leaves, an anti-aging mixture containing ω-3 fatty acids, unsaturated fatty acids, vitamin E, zinc and magnesium, flavonoids, etc. can be obtained, which can neutralize free radicals, reduce the oxidative damage of free radicals to cells, effectively delay cell aging, enhance the function of the immune system, and thus indirectly delay aging. Valerian, lemon balm, walnut, and lily are vacuum freeze-dried and powdered to obtain a sleep-aiding powder that can regulate nerve excitability, promote sleep, reduce tension and anxiety, and thus improve sleep quality. It can also promote the synthesis of melatonin, thereby improving sleep quality. Sucrose laurate is mixed with corn protein, glutaraldehyde, and 70% ethanol solution. A mixing reaction is carried out. Zein itself has a certain film-forming ability and contains rich amino acid residues. It can undergo a cross-linking reaction with glutaraldehyde to construct a three-dimensional network structure. Sucrose laurate reduces interfacial tension and promotes contact and reaction between zein and glutaraldehyde. At the same time, the long-chain alkyl portion in its molecular structure can be embedded in the cross-linked network of zein, further enhancing the stability and density of the network structure. Sucrose laurate has excellent emulsifying and solubilizing properties, which can increase the solubility and dispersibility of effective components in the gastrointestinal tract, thereby facilitating the absorption of these substances through the gastrointestinal mucosa. The resulting anti-aging and sleep-aiding composition can be slowly released in the gastrointestinal tract, improving its absorption efficiency.Mix hydroxypropyl cellulose, copovidone, tartaric acid, propyl gallate, and 70% ethanol solution. Hydroxypropyl cellulose has excellent water solubility and adhesion, forming a water-soluble gel layer that protects the cell-repairing anti-aging sleep-aiding preparation and prevents degradation during storage. This synergistically slows the release of the drug, helping the active ingredients in the cell-repairing anti-aging sleep-aiding preparation to dissolve and disperse better. The resulting plant extract telomerase factor cell-repairing anti-aging sleep-aiding compound exhibits stable efficacy, poses no burden to the liver and kidneys with long-term use, and maintains its effects over time. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] In order to more clearly illustrate the present invention or the technical solutions in the prior art, the following will be described in a clear and easy-to-understand manner with reference to the accompanying drawings. Obviously, the drawings described below are only for the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0034] Figure 1 This is a graph showing the cell viability results described in Experimental Example 1 of the present invention;

[0035] Figure 2 This is a graph showing the deep sleep time throughout the night as described in Experimental Example 1 of the present invention;

[0036] Figure 3 This is a graph showing the number of awakenings at night described in Experimental Example 1 of the present invention;

[0037] Figure 4 This is the metabolic index result diagram described in Experimental Example 1 of the present invention;

[0038] Figure 5 This is a graph showing the rat organ index results described in Experimental Example 2 of the present invention. DETAILED DESCRIPTION

[0039] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0040] Unless otherwise defined, all technical and scientific terms used herein have the same meanings as those familiar to those skilled in the art. Furthermore, any methods and materials similar or equivalent to those described herein can be applied to the present invention. The preferred embodiments and materials described herein are for illustrative purposes only and are not intended to limit the scope of this application.

[0041] The experimental methods in the following examples, unless otherwise specified, are conventional methods; the test materials and test strains used in the following examples, unless otherwise specified, are purchased from commercial channels.

[0042] Example 1: This example provides a plant extract telomerase factor repair cell anti-aging sleep-inducing composite preparation, the plant extract telomerase factor repair cell anti-aging sleep-inducing composite preparation comprising the following components in parts by weight:

[0043] 22 parts of repair cell extract preparation, 13 parts of anti-aging and sleep-aiding composition, 2.2 parts of hydroxypropyl cellulose, 0.6 parts of copovidone, 1.1 parts of tartaric acid, and 1.2 parts of propyl gallate.

[0044] The raw materials for preparing the repair cell extract preparation include the following components in parts by weight: 6 parts of strawberry seeds, 5 parts of grape peels, 2 parts of tomato leaves, 4 parts of wild ginseng, 3 parts of pomegranate seeds, 1.1 parts of Monascus, 2.5 parts of bile acid, 3 parts of L-lysine diisocyanate, and 1.5 parts of glycerol.

[0045] The raw materials for preparing the anti-aging and sleep-aiding composition include the following components in parts by weight: 4 parts of fish oil, 2 parts of pumpkin seeds, 3 parts of ginkgo leaves, 4 parts of valerian, 2.5 parts of lemon balm, 2 parts of walnuts, 3 parts of lilies, 2.6 parts of sucrose laurate, 2.5 parts of corn protein, and 1.1 parts of glutaraldehyde.

[0046] The preparation method of the repair cell extract preparation specifically comprises the following steps:

[0047] S1. Place strawberry seeds, grape peels, tomato leaves, wild ginseng, and pomegranate seeds in a 1.5 kW grinder at a grinding temperature of 23°C, a grinding time of 15 min, and a grinding speed of 3500 r / min. After grinding, place the crushed seeds and ultrapure water in a 3.5 kW fully automatic extractor at an extraction pressure of 12 MPa, an extraction temperature of 50°C, and an extraction time of 30 min to obtain a plant component containing telomerase factor activating protein;

[0048] S2. Inoculate Monascus into a 5% sterile glucose solution and place it in a constant temperature shaking incubator with a power of 2.2 kW. The constant temperature shaking incubation temperature is 33°C, the constant temperature shaking incubation time is 24 h, and the constant temperature shaking incubation speed is 270 r / min. After constant temperature shaking incubation, place it in an ultrasonic cell disruptor with a power of 1.2 kW, the ultrasonic disruption frequency is 20 kHz, the ultrasonic disruption temperature is 0°C, and the ultrasonic disruption time is 10 min. After disruption, filter using a 0.22 mm sterile filter membrane to obtain a Monascus extract;

[0049] S3, placing bile acid, L-lysine diisocyanate, glycerol, and dimethyl sulfoxide in a 2.4 kW reactor, reacting at a temperature of 100° C., a reaction rate of 300 r / min, and a reaction time of 8 h, mixing and performing a cross-linking reaction to obtain a carrier solution;

[0050] S4. Place the carrier solution prepared in S3, the plant component containing telomerase factor activating protein prepared in S1, and the Monascus extract prepared in S2 into a stirring tank with a power of 1.5 kW, stirring at a temperature of 35°C, a stirring time of 1 h, and a stirring speed of 400 r / min for sufficient adsorption to obtain a repair cell solution;

[0051] S5. Place the repair cell solution prepared in S4 into a vacuum freeze-nano spray dryer with a power of 2.3 kW, a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 2.5 h for freeze-spray drying to obtain a repair cell extract preparation.

[0052] In S1, the material-liquid ratio of the strawberry seeds to ultrapure water is 1:2 g / mL.

[0053] In S2, the Monascus was purchased from the China General Microbiological Culture Collection Center with a collection number of CGMCC3.7036. The material-liquid ratio of the Monascus to 5% sterile glucose solution was 1:100 g / mL.

[0054] In S3, the mass fraction of bile acid in dimethyl sulfoxide is 3%.

[0055] The preparation method of the anti-aging and sleep-aiding composition specifically comprises the following steps:

[0056] L1. Put fish oil, pumpkin seeds, and ginkgo leaves into a 1.5 kW refiner at a refining speed of 3000 r / min, a refining temperature of 28°C, and a refining time of 10 min to obtain an anti-aging mixture;

[0057] L2. Place valerian, lemon balm, walnut, and lily in a 5.5 kW vacuum freeze dryer with a vacuum degree of 60 Pa and a freezing temperature of -20°C for 2 hours. Then, heat the freeze dryer to 2°C and dry for 1.2 hours. Then, pulverize the freeze dryer using a 1.6 kW pulverizer at a temperature of 25°C for 20 minutes to obtain a sleep-inducing powder.

[0058] L3. Place sucrose laurate, zein, glutaraldehyde, and 70% ethanol solution in a 2.4 kW reactor at a reaction temperature of 40°C, a reaction rate of 300 r / min, and a reaction time of 6 h, and mix and react to obtain a mixture that promotes transdermal absorption;

[0059] L4, the anti-aging mixture prepared in L1 and the sleep-aiding powder prepared in L2 were placed in a 1.2 kW stirrer at a stirring temperature of 23°C, a stirring time of 20 min, and a stirring speed of 300 r / min. After mixing, the mixture was placed in a granulator with a power of 2.1 kW, a granulation temperature of 28°C, a granulation time of 30 min, and a granulation diameter of 1.2 mm for granulation to obtain the anti-aging and sleep-aiding component;

[0060] L5. Use a 1.6 kW sprayer to evenly spray the transdermal absorption promoting mixture prepared in L3 onto the surface of the anti-aging and sleep-aiding component prepared in L4. The mixture is placed at 25°C for 2 hours and air-dried to obtain an anti-aging and sleep-aiding composition.

[0061] In L3, the mass fraction of the sucrose laurate in the 70% ethanol solution is 5%.

[0062] The preparation method of the plant extract telomerase factor cell repair anti-aging sleep-inducing composite preparation specifically comprises the following steps:

[0063] Step 1: Place hydroxypropyl cellulose, copovidone, tartaric acid, propyl gallate, and 70% ethanol solution in a 1.2 kW stirrer at a stirring temperature of 23° C. for 10 min at a stirring speed of 500 r / min to obtain a stabilizing viscous component;

[0064] Step 2: Place the repair cell extract preparation and the anti-aging sleep-aiding composition into a stirring tank with a power of 1.5 kW, stir at a temperature of 20° C., a stirring speed of 300 r / min, and a stirring time of 18 min, and stir and mix to obtain the repair cell anti-aging sleep-aiding preparation;

[0065] Step 3: Place the stabilizing viscous component prepared in step 1 into the cell repair, anti-aging, and sleep-aiding preparation prepared in step 2, with a stirring temperature of 20°C, a stirring speed of 300 r / min, and a stirring time of 20 min. Mix thoroughly, place in a tablet press with a power of 1.8 kW, a tableting temperature of 20°C, a tableting time of 18 min, and a tableting diameter of 8 mm. After tableting, a plant extract telomerase factor cell repair, anti-aging, and sleep-aiding composite preparation is obtained.

[0066] In step 1, the mass fraction of the hydroxypropyl cellulose in the 70% ethanol solution is 2%.

[0067] Example 2: This example provides a plant extract telomerase factor repair cell anti-aging sleep-inducing composite preparation, the plant extract telomerase factor repair cell anti-aging sleep-inducing composite preparation comprising the following components in parts by weight:

[0068] 24 parts of repair cell extract preparation, 15 parts of anti-aging and sleep-aiding composition, 2.5 parts of hydroxypropyl cellulose, 0.8 parts of copovidone, 1.2 parts of tartaric acid, and 1.3 parts of propyl gallate.

[0069] The raw materials for preparing the repair cell extract preparation include the following components in parts by weight: 7 parts of strawberry seeds, 6 parts of grape peels, 2.5 parts of tomato leaves, 6 parts of wild ginseng, 5 parts of pomegranate seeds, 1.2 parts of Monascus, 3 parts of bile acid, 3.5 parts of L-lysine diisocyanate, and 1.6 parts of glycerol.

[0070] The raw materials for preparing the anti-aging and sleep-aiding composition include the following components in parts by weight: 5 parts of fish oil, 3 parts of pumpkin seeds, 5 parts of ginkgo leaves, 4.5 parts of valerian, 2.8 parts of lemon balm, 3 parts of walnuts, 4 parts of lily, 2.9 parts of sucrose laurate, 2.8 parts of corn protein, and 1.2 parts of glutaraldehyde.

[0071] The preparation method of the repair cell extract preparation specifically comprises the following steps:

[0072] S1. Place strawberry seeds, grape peels, tomato leaves, wild ginseng, and pomegranate seeds in a 1.6 kW grinder at a grinding temperature of 26°C, a grinding time of 18 min, and a grinding speed of 4000 r / min. After grinding, place the crushed seeds and ultrapure water in a 3.6 kW fully automatic extractor at an extraction pressure of 12 MPa, an extraction temperature of 50°C, and an extraction time of 35 min to obtain a plant component containing telomerase factor activating protein;

[0073] S2. Inoculate Monascus into a 5% sterile glucose solution and place it in a constant temperature shaking incubator with a power of 2.3 kW. The constant temperature shaking incubation temperature is 33°C, the constant temperature shaking incubation time is 28 h, and the constant temperature shaking incubation speed is 270 r / min. After constant temperature shaking incubation, place it in an ultrasonic cell disruptor with a power of 1.3 kW, the ultrasonic disruption frequency is 20 kHz, the ultrasonic disruption temperature is 0°C, and the ultrasonic disruption time is 12 min. After disruption, filter using a 0.22 mm sterile filter membrane to obtain a Monascus extract;

[0074] S3, placing bile acid, L-lysine diisocyanate, glycerol, and dimethyl sulfoxide in a 2.4 kW reactor, reacting at a temperature of 100° C., a reaction rate of 300 r / min, and a reaction time of 8 h, mixing and performing a cross-linking reaction to obtain a carrier solution;

[0075] S4. Place the carrier solution prepared in S3, the plant component containing telomerase factor activating protein prepared in S1, and the Monascus extract prepared in S2 into a stirring tank with a power of 1.5 kW, stirring at a temperature of 35°C, a stirring time of 1 h, and a stirring speed of 400 r / min for sufficient adsorption to obtain a repair cell solution;

[0076] S5. Place the repair cell solution prepared in S4 into a vacuum freeze-nano spray dryer with a power of 2.3 kW, a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 2.5 h for freeze-spray drying to obtain a repair cell extract preparation.

[0077] In S1, the material-liquid ratio of the strawberry seeds to ultrapure water is 1:2 g / mL.

[0078] In S2, the Monascus was purchased from the China General Microbiological Culture Collection Center with a collection number of CGMCC3.7036. The material-liquid ratio of the Monascus to 5% sterile glucose solution was 1:100 g / mL.

[0079] In S3, the mass fraction of bile acid in dimethyl sulfoxide is 3%.

[0080] The preparation method of the anti-aging and sleep-aiding composition specifically comprises the following steps:

[0081] L1. Put fish oil, pumpkin seeds, and ginkgo leaves into a 1.7 kW refiner at a refining speed of 3000 r / min, a refining temperature of 31°C, and a refining time of 13 min to obtain an anti-aging mixture;

[0082] L2. Place valerian, lemon balm, walnut, and lily in a 6.5 kW vacuum freeze dryer with a vacuum degree of 70 Pa and a freezing temperature of -20°C for 2.5 hours. Then, heat the freeze dryer to 3°C and dry for 1.3 hours. Then, pulverize the freeze dryer using a 1.9 kW pulverizer at a temperature of 26°C for 23 minutes to obtain a sleep-inducing powder.

[0083] L3. Place sucrose laurate, zein, glutaraldehyde, and 70% ethanol solution in a 2.4 kW reactor, react at a temperature of 45°C, a reaction rate of 300 r / min, and a reaction time of 6 h, and mix and react to obtain a mixture that promotes transdermal absorption;

[0084] L4, the anti-aging mixture prepared in L1 and the sleep-aiding powder prepared in L2 were placed in a 1.3 kW stirrer at a stirring temperature of 26°C, a stirring time of 25 min, and a stirring speed of 300 r / min. After mixing, the mixture was placed in a granulator with a power of 2.3 kW, a granulation temperature of 30°C, a granulation time of 35 min, and a granulation diameter of 1.5 mm for granulation to obtain the anti-aging and sleep-aiding component;

[0085] L5. Use a 2.1 kW sprayer to evenly spray the transdermal absorption promoting mixture prepared in L3 onto the surface of the anti-aging and sleep-aiding component prepared in L4. The mixture is placed at a temperature of 26°C for 2.3 hours and air-dried to obtain an anti-aging and sleep-aiding composition.

[0086] In L3, the mass fraction of the sucrose laurate in the 70% ethanol solution is 5%.

[0087] The preparation method of the plant extract telomerase factor cell repair anti-aging sleep-inducing composite preparation specifically comprises the following steps:

[0088] Step 1: Place hydroxypropyl cellulose, copovidone, tartaric acid, propyl gallate, and 70% ethanol solution in a 1.3 kW stirrer at a stirring temperature of 26°C for 12 minutes at a stirring speed of 500 r / min to obtain a stabilizing viscous component;

[0089] Step 2: Place the repair cell extract preparation and the anti-aging sleep-aiding composition into a stirring tank with a power of 1.5 kW, stir at a temperature of 20° C., a stirring speed of 300 r / min, and a stirring time of 18 min, and stir and mix to obtain the repair cell anti-aging sleep-aiding preparation;

[0090] Step 3: Place the stabilizing viscous component prepared in step 1 into the cell repair, anti-aging, and sleep-aiding preparation prepared in step 2, with a stirring temperature of 20°C, a stirring speed of 300 r / min, and a stirring time of 20 min. Mix thoroughly, place in a tablet press with a power of 2.1 kW, a tableting temperature of 23°C, a tableting time of 20 min, and a tableting diameter of 10 mm. After tableting, a plant extract telomerase factor cell repair, anti-aging, and sleep-aiding composite preparation is obtained.

[0091] In step 1, the mass fraction of the hydroxypropyl cellulose in the 70% ethanol solution is 2%.

[0092] Example 3: This example provides a plant extract telomerase factor repair cell anti-aging sleep-inducing composite preparation, the plant extract telomerase factor repair cell anti-aging sleep-inducing composite preparation comprising the following components in parts by weight:

[0093] 26 parts of repair cell extract preparation, 17 parts of anti-aging and sleep-aiding composition, 2.8 parts of hydroxypropyl cellulose, 0.9 parts of copovidone, 1.4 parts of tartaric acid, and 1.5 parts of propyl gallate.

[0094] The raw materials for preparing the repair cell extract preparation include the following components in parts by weight: 8 parts of strawberry seeds, 7 parts of grape peels, 3 parts of tomato leaves, 8 parts of wild ginseng, 7 parts of pomegranate seeds, 1.3 parts of Monascus, 3.5 parts of bile acid, 4 parts of L-lysine diisocyanate, and 1.8 parts of glycerol.

[0095] The raw materials for preparing the anti-aging and sleep-aiding composition include the following components in parts by weight: 6 parts of fish oil, 4 parts of pumpkin seeds, 6 parts of ginkgo leaves, 5 parts of valerian, 3 parts of lemon balm, 4 parts of walnuts, 4.5 parts of lilies, 3.2 parts of sucrose laurate, 3.2 parts of corn protein, and 1.3 parts of glutaraldehyde.

[0096] The preparation method of the repair cell extract preparation specifically comprises the following steps:

[0097] S1. Place strawberry seeds, grape peels, tomato leaves, wild ginseng, and pomegranate seeds in a 1.8 kW grinder at a grinding temperature of 28°C, a grinding time of 20 min, and a grinding speed of 4500 r / min. After grinding, place the crushed seeds and ultrapure water in a 3.8 kW fully automatic extractor at an extraction pressure of 12 MPa, an extraction temperature of 50°C, and an extraction time of 40 min to obtain a plant component containing telomerase factor activating protein;

[0098] S2. Inoculate Monascus into a 5% sterile glucose solution and place it in a constant temperature shaking incubator with a power of 2.6 kW, the constant temperature shaking incubation temperature is 33°C, the constant temperature shaking incubation time is 30 h, and the constant temperature shaking incubation speed is 270 r / min. After constant temperature shaking incubation, place it in an ultrasonic cell disruptor with a power of 1.5 kW, the ultrasonic disruption frequency is 20 kHz, the ultrasonic disruption temperature is 0°C, and the ultrasonic disruption time is 13 min. After disruption, filter using a 0.22 mm sterile filter membrane to obtain a Monascus extract;

[0099] S3, placing bile acid, L-lysine diisocyanate, glycerol, and dimethyl sulfoxide in a 2.4 kW reactor, reacting at a temperature of 100° C., a reaction rate of 300 r / min, and a reaction time of 8 h, mixing and performing a cross-linking reaction to obtain a carrier solution;

[0100] S4. Place the carrier solution prepared in S3, the plant component containing telomerase factor activating protein prepared in S1, and the Monascus extract prepared in S2 into a stirring tank with a power of 1.5 kW, stirring at a temperature of 35°C, a stirring time of 1 h, and a stirring speed of 400 r / min for sufficient adsorption to obtain a repair cell solution;

[0101] S5. Place the repair cell solution prepared in S4 into a vacuum freeze-nano spray dryer with a power of 2.3 kW, a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 2.5 h for freeze-spray drying to obtain a repair cell extract preparation.

[0102] In S1, the material-liquid ratio of the strawberry seeds to ultrapure water is 1:2 g / mL.

[0103] In S2, the Monascus was purchased from the China General Microbiological Culture Collection Center with a collection number of CGMCC3.7036. The material-liquid ratio of the Monascus to 5% sterile glucose solution was 1:100 g / mL.

[0104] In S3, the mass fraction of bile acid in dimethyl sulfoxide is 3%.

[0105] The preparation method of the anti-aging and sleep-aiding composition specifically comprises the following steps:

[0106] L1. Put fish oil, pumpkin seeds, and ginkgo leaves into a 1.8 kW refiner at a refining speed of 3000 r / min, a refining temperature of 33°C, and a refining time of 15 min to obtain an anti-aging mixture;

[0107] L2. Place valerian, lemon balm, walnut, and lily in a 7.5 kW vacuum freeze dryer with a vacuum degree of 80 Pa and a freezing temperature of -20°C for 3 hours. Then, heat the freeze dryer to 4°C and dry for 1.5 hours. Then, pulverize the freeze dryer using a 2.2 kW pulverizer at a temperature of 28°C for 25 minutes to obtain a sleep-inducing powder.

[0108] L3. Place sucrose laurate, zein, glutaraldehyde, and 70% ethanol solution in a 2.4 kW reactor at a reaction temperature of 50°C, a reaction rate of 300 r / min, and a reaction time of 6 h, and mix and react to obtain a mixture that promotes transdermal absorption;

[0109] L4, the anti-aging mixture prepared in L1 and the sleep-aiding powder prepared in L2 were placed in a 1.4 kW stirrer at a stirring temperature of 28° C., a stirring time of 30 min, and a stirring speed of 300 r / min. After mixing, the mixture was placed in a granulator with a power of 2.4 kW, a granulation temperature of 32° C., a granulation time of 45 min, and a granulation diameter of 1.6 mm for granulation to obtain the anti-aging and sleep-aiding component;

[0110] L5. Use a 2.3 kW sprayer to evenly spray the transdermal absorption promoting mixture prepared in L3 onto the surface of the anti-aging and sleep-aiding component prepared in L4. The mixture is placed at a temperature of 28°C for 2.5 hours and air-dried to obtain an anti-aging and sleep-aiding composition.

[0111] In L3, the mass fraction of the sucrose laurate in the 70% ethanol solution is 5%.

[0112] The preparation method of the plant extract telomerase factor cell repair anti-aging sleep-inducing composite preparation specifically comprises the following steps:

[0113] Step 1: Place hydroxypropyl cellulose, copovidone, tartaric acid, propyl gallate, and 70% ethanol solution in a 1.4 kW stirrer at a stirring temperature of 28°C for 15 minutes at a stirring speed of 500 r / min to obtain a stabilizing viscous component;

[0114] Step 2: Place the repair cell extract preparation and the anti-aging sleep-aiding composition into a stirring tank with a power of 1.5 kW, stir at a temperature of 20° C., a stirring speed of 300 r / min, and a stirring time of 18 min, and stir and mix to obtain the repair cell anti-aging sleep-aiding preparation;

[0115] Step 3: Place the stabilizing viscous component prepared in step 1 into the cell repair, anti-aging, and sleep-aiding preparation prepared in step 2, with a stirring temperature of 20°C, a stirring speed of 300 r / min, and a stirring time of 20 min. Mix thoroughly, place in a tablet press with a power of 2.2 kW, a tableting temperature of 25°C, a tableting time of 22 min, and a tableting diameter of 11 mm. After tableting, a plant extract telomerase factor cell repair, anti-aging, and sleep-aiding composite preparation is obtained.

[0116] In step 1, the mass fraction of the hydroxypropyl cellulose in the 70% ethanol solution is 2%.

[0117] Comparative Example 1: This comparative example provides a cell repair anti-aging sleep-aiding composite preparation and a preparation method thereof. The only difference from Example 1 is that the added cell repair extract preparation does not contain a carrier solution, and the remaining components, component contents, and method steps are the same as those in Example 1.

[0118] Comparative Example 2: This comparative example provides a cell repair anti-aging sleep-aiding composite preparation and a preparation method thereof. The only difference from Example 1 is that the added anti-aging sleep-aiding composition does not contain a transdermal absorption-promoting mixture, and the remaining components, component contents, and method steps are the same as those in Example 1.

[0119] Experimental Example 1: Test to determine the efficacy of cell repair, anti-aging and sleep-inducing properties.

[0120] The plant extract telomerase factor cell repair, anti-aging and sleep-inducing composite preparation prepared in Examples 1-3 of the present invention, and the test steps for determining the cell repair, anti-aging and sleep-inducing efficacy are as follows:

[0121] (1) 10 mL of a solution with a density of 5×10 5 Fibroblasts of 100 cells / mL were seeded in the wells of a 96-well plate and cultured in a constant temperature incubator at 37°C and 5% CO2. The culture medium was IMDM, 20 mM glutamine was added, and 10% fetal bovine serum was added. The culture was carried out for 24 h. 1.6 mM hydrogen peroxide and the plant extract telomerase factor repair cell anti-aging and sleep-aiding composite preparation prepared in Example 1-3, the repair cell anti-aging and sleep-aiding composite preparation prepared in Comparative Example 1-2, and a control group of commercially available cell repair preparations (purchased from Guangzhou Baicao Zhengyuan Biological Research Co., Ltd.) were added to the fresh culture medium at the same time. 10 mg of each was used as Example 1-3 group, Comparative Example 1-2 group, and control group. The culture was continued for 24 h, and then 20 mL of MTT was added to each well. After culturing for 4 h, the culture medium was discarded and 150 mL of DMSO was added. The absorbance value was detected at a wavelength of 490 nm on a microplate reader. The test was repeated 3 times for each group, and the average value was taken. The cell viability was expressed as a percentage.

[0122] (2) 60 subjects aged 50-55 years were randomly selected. All of them had insomnia symptoms. The average deep sleep time throughout the night measured by a sleep monitoring instrument was 1 hour, and the number of awakenings in the middle of the night was more than 3 times. They were divided into 6 groups, with 10 subjects in each group. Before the start, the average metabolic index of the subjects in each group was measured using a metabolic syndrome index measurement tool. The subjects in Example 1-3 groups took the plant extract telomerase factor repair cell anti-aging sleep-aiding composite preparation prepared in Example 1-3, the subjects in Comparative Example 1-2 groups took the repair cell anti-aging sleep-aiding composite preparation prepared in Comparative Example 1-2, and the subjects in the control group took the commercially available cell repair preparation in the control group. The daily dose for each person was 1 g. After taking the preparation for 30 days, the average deep sleep time throughout the night and the average number of awakenings in the middle of the night of the subjects in Example 1-3 groups, Comparative Example 1-2 groups, and the control group were measured and recorded by sleep monitoring. The average metabolic index of the subjects in Example 1-3 groups, Comparative Example 1-2 groups, and the control group was measured using a metabolic syndrome index measurement tool. The subjects stopped taking the preparation for 30 days. d after stopping the drug, the average deep sleep time throughout the night and the average number of awakenings in the middle of the night of the subjects in Example 1-3 Group, Comparative Example 1-2 Group, and Control Group were recorded. The metabolic syndrome index measurement tool was used to measure the average metabolic index of the subjects in Example 1-3 Group, Comparative Example 1-2 Group, and Control Group 30 d after stopping the drug and recorded.

[0123] Result analysis:

[0124] Figure 1 This is a graph showing the cell survival rate results of Experimental Example 1 of the present invention. As shown in the figure, the plant extract telomerase factor repair cell anti-aging and sleep-aiding composite preparations prepared in Examples 1-3 were added to fibroblasts, and after culture, the cell survival rates were 92%, 94%, and 96%, respectively. The repair cell anti-aging and sleep-aiding composite preparations prepared in Comparative Examples 1-2 were added to fibroblasts, and after culture, the cell survival rates were 80% and 87%, respectively. In the control group, the commercially available cell repair preparation was added to fibroblasts, and after culture, the cell survival rate was 44%; Figure 2 This is the result diagram of the deep sleep time throughout the night described in Experimental Example 1 of the present invention. Figure 3 This is the result graph of the number of awakenings in the middle of the night described in Experimental Example 1 of the present invention. Figure 4 This is the metabolic index result diagram described in Experimental Example 1 of the present invention, as shown in FIG. Figure 2 、 Figure 3 、 Figure 4As shown, after the subjects in Example 1-3 groups took the plant extract telomerase factor repair cell anti-aging sleep-aiding composite preparation prepared in Example 1-3 for 30 days, the average deep sleep time throughout the night was 2 h, 2.2 h, and 2.4 h, the average number of awakenings in the middle of the night was 2.2 times, 2 times, and 1.8 times, and the metabolic indexes were 1091, 1105, and 1140, respectively. After stopping taking the preparation for 30 days, the average deep sleep time throughout the night of the subjects in Example 1-3 groups was 2 h, 2.2 h, and 2.4 h, the average number of awakenings in the middle of the night was 2.2 times, 2.1 times, and 1.8 times, respectively, and the metabolic indexes were 1088, 1097, and 1135, respectively. After the subjects in Comparative Example 1-2 groups took the plant extract telomerase factor repair cell anti-aging sleep-aiding composite preparation prepared in Comparative Example 1-2 for 30 days, the average deep sleep time throughout the night was 1.9 h and 1.7 h, with an average number of awakenings in the middle of the night of 1.9 times and 2.5 times, and metabolic indexes of 1024 and 1036, respectively. 30 days after stopping taking the drug, the average deep sleep time of the subjects in groups 1 and 2 of the control group were 1.8 h and 1.6 h, with an average number of awakenings in the middle of the night of 2 times and 2.7 times, respectively, and metabolic indexes of 1009 and 1024, respectively. After taking the commercially available cell repair preparation for 30 days, the subjects in the control group had an average deep sleep time of 1.5 h, an average number of awakenings in the middle of the night of 3.4 times, and a metabolic index of 1053. 30 days after stopping taking the drug, the subjects in the control group had an average deep sleep time of 1.2 h, an average number of awakenings in the middle of the night of 3.8 times, and a metabolic index of 1021, indicating that the plant extract telomerase factor repair cell anti-aging sleep-aiding composite preparation of the present invention has a stable effect and can maintain the effect for a long time.

[0125] Experimental Example 2: Long-term consumption does not burden the liver and kidneys

[0126] The test steps for determining whether the plant extract telomerase factor cell repair, anti-aging and sleep-promoting composite preparation prepared in Examples 1-3 of the present invention produces no burden on the liver and kidneys after long-term consumption are as follows:

[0127] (1) Sixty male SD rats, each weighing 220 g (purchased from Chengdu Dashuo Animal Co., Ltd.), were randomly divided into six groups of 10 rats each. The rats were housed in separate cages, which were kept clean and dry at a temperature of 24°C and a humidity of 50%, with free access to food and water.

[0128] (2) After 10 days of feeding, rats in Example 1-3 group were fed with the plant extract telomerase factor repair cell anti-aging sleep-aiding compound prepared in Example 1-3, rats in Comparative Example 1-2 group were fed with the repair cell anti-aging sleep-aiding compound prepared in Comparative Example 1-2 group, and rats in the control group were fed with the control group commercial cell repair preparation (purchased from Guangzhou Baicao Zhengyuan Biological Research Co., Ltd.), 1 g of which was consumed every day for 70 consecutive days. After 70 days, the rats were fasted for 24 hours and killed by cervical dislocation. The liver and kidney of the rats were separated, rinsed with 0.9% saline, and dried with filter paper. The liver and kidney were quickly weighed. The organ index (%) = weight of a certain organ / rat body weight × 100%. An increase in the organ index indicates congestion, edema or hyperplasia of the organ, while a decrease in the organ index indicates organ atrophy and other degenerative changes.

[0129] Result analysis:

[0130] Figure 5 This is the result graph of the rat organ index described in Experimental Example 2 of the present invention. The rats in Example 1-3 groups consumed the plant extract telomerase factor repair cell anti-aging sleep-aiding composite preparation prepared in Example 1-3, consuming 1 g per day. After consuming for 70 consecutive days, the rat liver indexes were 0.42, 0.50, and 0.52, respectively, and the rat kidney indexes were 1.03, 1.07, and 1.10, respectively. The rats in the comparative example 1-2 group consumed the repair cell anti-aging sleep-aiding composite preparation prepared in the comparative example 1-2 group consumed 1 g per day. After consuming for 70 consecutive days, the rat liver indexes were 0.44 and 0.45, respectively, and the rat kidney indexes were 1.05 and 1.06, respectively. The rats in the control group consumed the commercially available cell repair preparation of the control group, consuming 1 g per day. After consuming for 70 consecutive days, the rat liver indexes were 0.44 and 0.45, respectively, and the rat kidney indexes were 1.05 and 1.06, respectively. After d, the liver index of the rats was 0.85, and the kidney index of the rats was 1.44. The organ indexes of the rats in the control group increased, indicating that the organs were congested, edematous, or hypertrophic. This shows that the plant extract telomerase factor repair cell anti-aging and sleep-aiding composite preparation of the present invention has a stable effect, does not cause a burden on the liver and kidneys when used for a long time, and has no side effects on the organs after taking it.

[0131] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

[0132] The present invention and its embodiments are described above. Such description is not restrictive. The drawings show only one embodiment of the present invention, and actual applications are not limited thereto. In short, if a person skilled in the art is inspired by the above, and does not deviate from the purpose of the present invention, any method and embodiment similar to the technical solution without creative design shall fall within the scope of protection of the present invention.

Claims

1. A plant extract telomerase factor cell repair anti-aging sleep aid compound preparation, characterized in that: The plant extract telomerase factor cell repair, anti-aging and sleep-inducing composite preparation specifically comprises the following components in parts by weight: 22-26 parts of a repair cell extract preparation, 13-17 parts of an anti-aging and sleep-aiding composition, 2.2-2.8 parts of hydroxypropyl cellulose, 0.6-0.9 parts of copovidone, 1.1-1.4 parts of tartaric acid, and 1.2-1.5 parts of propyl gallate; The raw materials for preparing the repair cell extract preparation include the following components in parts by weight: 6-8 parts of strawberry seeds, 5-7 parts of grape peels, 2-3 parts of tomato leaves, 4-8 parts of wild ginseng, 3-7 parts of pomegranate seeds, 1.1-1.3 parts of Monascus, 2.5-3.5 parts of bile acid, 3-4 parts of L-lysine diisocyanate, and 1.5-1.8 parts of glycerol; The raw materials for preparing the anti-aging and sleep-aiding composition include the following components in parts by weight: 4-6 parts of fish oil, 2-4 parts of pumpkin seeds, 3-6 parts of ginkgo leaves, 4-5 parts of valerian, 2.5-3 parts of lemon balm, 2-4 parts of walnuts, 3-4.5 parts of lilies, 2.6-3.2 parts of sucrose laurate, 2.5-3.2 parts of corn protein, and 1.1-1.3 parts of glutaraldehyde; The preparation method of the repair cell extract preparation specifically comprises the following steps: S1. Crush strawberry seeds, grape peels, tomato leaves, wild ginseng, and pomegranate seeds, and extract to obtain plant components containing telomerase factor activating protein; S2, inoculating Monascus into 5% sterile glucose solution, culturing, and then ultrasonically crushing and filtering to obtain a Monascus extract; S3, mixing bile acid with L-lysine diisocyanate, glycerol, and dimethyl sulfoxide to react to obtain a carrier solution; S4, mixing the carrier solution prepared in S3 with the plant component containing telomerase factor activating protein prepared in S1 and the Monascus extract prepared in S2, and performing sufficient adsorption to obtain a repair cell solution; S5, freeze-spray drying the repair cell solution prepared in S4 to obtain a repair cell extract preparation; The preparation method of the anti-aging and sleep-aiding composition specifically comprises the following steps: L1. Grind fish oil, pumpkin seeds, and ginkgo leaves to obtain an anti-aging mixture; L2. Freeze-dry valerian, lemon balm, walnut, and lily, and grind them into powder to obtain a sleep-inducing powder; L3, mixing sucrose laurate with zein, glutaraldehyde, and 70% ethanol solution to obtain a mixture that promotes transdermal absorption; L4, mixing the anti-aging mixture prepared in L1 and the sleep-aiding powder prepared in L2, and granulating them to obtain an anti-aging and sleep-aiding component; L5. Spray the mixture prepared in L3 to promote transdermal absorption evenly on the surface of the anti-aging and sleep-aiding component prepared in L4, place it at a temperature of 25-28°C for 2-2.5 hours, and air-dry to obtain the anti-aging and sleep-aiding composition.

2. The plant extract telomerase factor cell repair, anti-aging and sleep-inducing composite preparation according to claim 1, characterized in that: The preparation method of the plant extract telomerase factor cell repair anti-aging sleep-inducing composite preparation specifically comprises the following steps: Step 1: Mix hydroxypropyl cellulose, copovidone, tartaric acid, propyl gallate, and 70% ethanol solution to obtain a stabilizing viscous component; Step 2: Stirring and mixing the repair cell extract preparation and the anti-aging and sleep-aiding composition to obtain the repair cell anti-aging and sleep-aiding preparation; Step 3: Add the stabilizing viscous component prepared in step 1 to the cell repair, anti-aging, and sleep-aiding preparation prepared in step 2, mix them thoroughly, and compress them into tablets to obtain a plant extract telomerase factor cell repair, anti-aging, and sleep-aiding composite preparation.

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