Reagent container for nucleic acid extraction and nucleic acid amplification

Abstract

The invention relates to a reagent container for nucleic acid extraction and nucleic acid amplification, which comprises a box body and a box cover, and the box body is provided with a sample cavity and a reagent cavity; the kit cover comprises an upper cover and a lower cover, a reagent through hole communicated with the reagent cavity and a sample injection hole communicated with the reagent cavity are formed in a base plate of the lower cover, and the reagent through hole and the sample injection hole are sealed by sealing films; an upper disc of the upper cover is provided with a sample inlet hole and a sealing hole; a puncture needle is arranged at the bottom of the upper disc; when the upper cover is located at the first screwing position, a gap is formed between the puncture needle and the sealing film; when the upper cover is located at the second screwing position, the sample injection hole is opposite to the sample injection hole, and the puncture needle punctures the sealing film located at the reagent through hole; when the upper cover is located at the third screwing position or returns to the first screwing position, the sealing hole is opposite to the sample injection hole, and the sample injection hole is blocked through the sealing plug. The technical problems that a box cover for nucleic acid extraction and nucleic acid amplification is inconvenient to open and close, and the box cover is not tightly closed and easily leaks are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of molecular diagnosis, and in particular to a reagent container for nucleic acid extraction and nucleic acid amplification. Background Art

[0002] Molecular diagnostic technology uses DNA and RNA as diagnostic materials and molecular biology techniques to diagnose human conditions and diseases by detecting the presence, defects, or abnormal expression of genes. Its basic principle is to detect changes in the structure, quantity, and expression function of DNA or RNA to determine whether the subject has abnormal genetic changes. This is of great significance for the prevention, prediction, diagnosis, treatment, and prognosis of diseases. In simple terms, all methodological techniques based on molecular biology, such as polymerase chain reaction (PCR) and gene sequencing, are considered molecular diagnostic technologies.

[0003] To complete molecular diagnostic testing on the sample to be tested, it is necessary to perform nucleic acid extraction and nucleic acid amplification steps on the sample to be tested. Nucleic acid extraction refers to the release of nucleic acid (DNA or RNA) from cells or viruses and the removal of impurities other than nucleic acid by using various reagents and mechanical operations of instruments; nucleic acid amplification refers to the replication and amplification of the extracted nucleic acid through PCR technology.

[0004] The instruments required to perform nucleic acid extraction and nucleic acid amplification steps on the test sample include nucleic acid extractors, constant temperature amplification instruments, etc. With the development and progress of technology, instruments with integrated nucleic acid extraction and nucleic acid amplification functions have emerged in recent years, that is, nucleic acid extraction and nucleic acid amplification steps can be completed on one instrument. There are various containers for containing test samples and reagents required for nucleic acid extraction and nucleic acid amplification that are matched with such instruments. There are reagent strips, boxes, and split containers (multiple containers, each containing different reagents and test samples). Among them, the box-shaped container usually includes a box body and a box lid, which is connected to the box body in a hinged manner. A plurality of reagent cavities are formed on the box body, and various reagents can be contained in the reagent cavity. The top opening of the reagent cavity is sealed by a sealing film and is pressed under the box lid. When in use, the operator needs to first open the box lid, tear off the sealing film, inject the test sample, and then cover the box lid. Finally, the box-shaped container is placed on the instrument to perform nucleic acid extraction and nucleic acid amplification operations.

[0005] This type of box-shaped container has complicated operating steps when in use, and because the box lid and the box body need to be tightly closed, there are operational difficulties when opening and closing the box lid. Therefore, the operator not only needs two hands to cooperate with each other when opening and closing the box lid, but also needs to exert a large force to lift or close the box lid, resulting in the disadvantages of poor user experience, time-consuming and labor-intensive use of this type of box-shaped container. In addition, the box lid of this type of box-shaped container is prone to not closing tightly when closing, resulting in leakage of reagents in the reagent chamber of the box body, thereby affecting subsequent test results.

[0006] Therefore, the present invention provides a reagent container for nucleic acid extraction and nucleic acid amplification to overcome the defects of the prior art. Summary of the Invention

[0007] The purpose of the present invention is to provide a reagent container for nucleic acid extraction and nucleic acid amplification, which is easy to use and the operator can open and close the box cover and open the sealing film with one hand, giving the operator a better experience.

[0008] Another object of the present invention is to provide a reagent container for nucleic acid extraction and nucleic acid amplification, which can be opened and closed and the sealing membrane can be opened by twisting the box lid, and there will be no leakage due to loose fastening, and it has better sealing performance.

[0009] The purpose of the present invention can be achieved by adopting the following scheme:

[0010] The present invention provides a reagent container for nucleic acid extraction and nucleic acid amplification, comprising:

[0011] A box body having a plurality of accommodating chambers with an open top and a sealed bottom, wherein the plurality of accommodating chambers are respectively configured as a sample chamber for storing samples to be tested and a reagent chamber for storing reagents required for nucleic acid extraction and amplification;

[0012] The box cover comprises an upper cover and a lower cover, wherein:

[0013] The lower cover includes a base plate, which is arranged at the top opening of the box body through the base plate. The base plate has a reagent through hole and a sample injection hole, the reagent through hole is connected to the reagent cavity, and the sample injection hole is connected to the sample cavity. A sealing film is provided on the top of the base plate to seal the reagent through hole and the sample injection hole;

[0014] The upper cover comprises an upper plate, the upper plate having an injection hole and a sealing hole, a sealing plug is provided at the sealing hole, and at least one puncture needle is provided at the bottom of the upper plate;

[0015] The upper cover is screwed together with the lower cover, and the upper cover has at least a first screwing position, a second screwing position, and a third screwing position relative to the lower cover. When the upper cover is in the first screwing position, the sealing hole is opposite to the injection hole, and a gap is formed between the puncture needle and the sealing film; when the upper cover is screwed down to the second screwing position, the injection hole is opposite to the injection hole, and the puncture needle punctures the sealing film at the reagent through-hole; when the upper cover is screwed down to the third screwing position or is screwed back to the first screwing position, the sealing hole is opposite to the injection hole, and the sealing plug arranged at the sealing hole blocks the injection hole.

[0016] In a preferred embodiment of the present invention, the top of the chassis has a lower outer ring and a lower inner ring arranged coaxially, the lower outer ring is arranged around the outer circumference of the lower inner ring, the reagent through hole is located on the inner side of the lower inner ring, and the sample injection hole is located between the lower outer ring and the lower inner ring;

[0017] The lower outer ring and the lower inner ring both have thread structures.

[0018] In a preferred embodiment of the present invention, there are multiple reagent through holes, and the multiple reagent through holes correspond one-to-one to the multiple reagent chambers.

[0019] In a preferred embodiment of the present invention, the bottom of the upper plate has an upper outer ring and an upper inner ring arranged coaxially, and the upper outer ring is arranged on the outer circumference of the upper inner ring;

[0020] The upper outer ring and the upper inner ring both have a threaded structure. When the upper cover and the lower cover are screwed together, the lower outer ring and the upper outer ring are screwed together via the threaded structure, and the lower inner ring and the upper inner ring are screwed together via the threaded structure.

[0021] In a preferred embodiment of the present invention, the sealing hole and the injection hole have different diameters, and are connected to the injection hole through the sealing hole or the injection hole to accommodate injection tools of different sizes.

[0022] In a preferred embodiment of the present invention, the upper plate is provided with a breathable grid, and after the puncture needle pierces the sealing film located at the reagent through-hole, the reagent chamber is connected to the outside world through the reagent through-hole and the breathable grid opposite thereto in sequence.

[0023] In a preferred embodiment of the present invention, when the upper cover is screwed down relative to the lower cover to the second screwing position, an injection tool is inserted through the injection hole and punctures the sealing film at the injection hole to inject the sample to be tested into the sample chamber.

[0024] In a preferred embodiment of the present invention, at least one of the accommodating chambers is configured as a plunger chamber, and the sample chamber and the reagent chamber are arranged around the periphery of the plunger chamber;

[0025] The bottom of the upper plate has a plunger coaxially arranged with the upper outer ring and the upper inner ring, and the plunger is located on the inner side of the upper inner ring;

[0026] The chassis is provided with a plunger hole coaxially arranged with the lower outer ring and the lower inner ring. The plunger hole is communicated with the plunger cavity, and the plunger can be movably inserted into the plunger hole.

[0027] In a preferred embodiment of the present invention, an annular boss is provided on the inner side of the lower inner ring and on the outer periphery of the plunger hole, and the reagent through hole is provided on the annular boss;

[0028] The sealing film for sealing the reagent through hole is arranged on the top surface of the annular boss.

[0029] In a preferred embodiment of the present invention, the bottom wall of the box body is provided with a plurality of liquid holes, and the plurality of liquid holes correspond to and communicate with the plurality of accommodating cavities one by one;

[0030] A flow channel switching valve is provided at the bottom of the box body, and the flow channel switching valve has a transfer storage chamber, and the transfer storage chamber is communicated with the plunger chamber. The flow channel switching valve is provided with a first inlet and a second inlet and a second inlet that are communicated with the transfer storage chamber. The flow channel switching valve can be driven to rotate around the axis of the plunger chamber so that one of the first inlet and the second inlet is communicated with one of the multiple liquid holes.

[0031] As described above, the characteristics and advantages of the reagent container for nucleic acid extraction and nucleic acid amplification of the present invention are:

[0032] A sample chamber for storing a sample to be tested and a reagent chamber for storing reagents required for nucleic acid extraction and amplification are formed in the box body. A box cover is provided on the top cover of the box body. The box cover is divided into two parts (i.e., an upper cover and a lower cover) that are screwed together. During use, the upper cover is in a first screwing position in the initial state. At this time, the sealing hole on the upper cover is opposite to the injection hole on the lower cover, and there is a gap between the puncture needle and the sealing film (the sealing film at the injection hole is not punctured), which can ensure a stable seal for the reagent chamber; when the upper cover is screwed down to the second screwing position, the injection hole on the upper cover is rotated to align with the injection hole on the lower cover. In contrast, at this time, the injection tool is inserted through the injection hole and the sealing membrane at the injection hole is punctured to inject the sample to be tested into the sample chamber; in the process of screwing the upper cover downward, the puncture needle at the bottom of the upper cover will puncture the sealing membrane at the reagent through hole as it rotates downward, and each reagent chamber can be connected to the outside world for subsequent nucleic acid extraction and nucleic acid amplification operations; when the upper cover is continued to be screwed down to the third screwing position (of course, the upper cover can also be screwed up to return to the first screwing position), so that the sealing hole is opposite to the injection hole again, a sealing plug can be added to the sealing hole at this time, and the sealing plug blocks the injection hole. Compared with the hinged box cover, the operator only needs to screw the upper cover with one hand to complete the opening and closing of the box cover and the opening of the sealing membrane. The operation is convenient and gives the operator a better experience.

[0033] In addition, during the entire operation process, the box lid of the present application is always covered on the top of the box body. The opening and closing and the opening of the sealing film can be completed by screwing the upper lid. There will be no leakage due to the box lid not being tightly fastened during the opening or closing process, and a good seal is always maintained between the box lid and the box body. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] The following drawings are only intended to illustrate and explain the present invention, and are not intended to limit the scope of the present invention.

[0035] in:

[0036] Figure 1 A perspective view of a reagent container for nucleic acid extraction and nucleic acid amplification according to the present invention in an unused state;

[0037] Figure 2 A perspective view of a reagent container for nucleic acid extraction and nucleic acid amplification according to the present invention in a used state;

[0038] Figure 3 This is one of the exploded views of the reagent container for nucleic acid extraction and nucleic acid amplification of the present invention;

[0039] Figure 4 This is the second exploded view of the reagent container for nucleic acid extraction and nucleic acid amplification of the present invention;

[0040] Figure 5 This is an exploded view of the box body portion of the reagent container for nucleic acid extraction and nucleic acid amplification of the present invention;

[0041] Figure 6 Schematic cross-sectional view of the box body portion of the reagent container for nucleic acid extraction and nucleic acid amplification of the present invention;

[0042] Figure 7 The figure is a schematic structural diagram of a flow channel switching valve in a reagent container for nucleic acid extraction and nucleic acid amplification according to the present invention.

[0043] The accompanying drawings in the present invention are:

[0044] 1. Box body; 101. Accommodating cavity;

[0045] 1011. Reagent chamber; 1012. Sample chamber;

[0046] 1013, plunger cavity; 102, liquid hole;

[0047] 2. Box cover; 201. Upper cover;

[0048] 2011, Upper Plate; 2012, Upper Outer Ring;

[0049] 2013, upper inner ring; 2014, injection hole;

[0050] 2015, sealing hole; 2016, sealing plug;

[0051] 2017, puncture needle; 2018, breathable grid;

[0052] 2019, plunger; 202, lower cover;

[0053] 2021, chassis; 2022, lower outer ring;

[0054] 2023, lower inner ring; 2024, reagent through hole;

[0055] 2025, injection hole; 2026, plunger hole;

[0056] 2027, annular boss; 3, flow channel switching valve;

[0057] 301. Transfer storage chamber; 302. First entrance / exit;

[0058] 303. Second inlet and outlet; 304. Cylinder;

[0059] 305, valve seat; 306, ultrasonic chamber;

[0060] 307. Soft rubber plug; 308. Driving rod. DETAILED DESCRIPTION

[0061] The technical solution of the present invention will be described in detail below with reference to the accompanying drawings and specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention. After reading the present invention, modifications of various equivalent forms of the present invention by those skilled in the art all fall within the scope defined by the claims attached to this application.

[0062] It should be noted that when an element is referred to as being "disposed on" another element, it may be directly on the other element or there may be an element centered thereon. When an element is referred to as being "connected to" another element, it may be directly connected to the other element or there may be an element centered thereon. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only implementations.

[0063] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this application pertains. The terms used herein in the specification of this application are intended only to describe specific embodiments and are not intended to limit this application. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0064] like Figures 1 to 4 As shown, the present invention provides a reagent container for nucleic acid extraction and nucleic acid amplification, which comprises a box body 1 with an open top and a sealed bottom, and a box cover 2 arranged at the top opening of the box body 1. The box body 1 has a plurality of accommodating chambers 101 with an open top and a sealed bottom. The plurality of accommodating chambers 101 are respectively configured as sample chambers 1012 for storing samples to be tested and reagent chambers 1011 for storing reagents required for nucleic acid extraction and amplification; the box cover 2 comprises an upper cover 201 and a lower cover 202, wherein: the lower cover 202 comprises a bottom plate 2021, and the lower cover 202 is covered with the top opening of the box body 1 through the bottom plate 2021. The bottom plate 2021 has a reagent through hole 2024 and a sample injection hole 2025, the reagent through hole 2024 is connected to the reagent chamber 1011, and the sample injection hole 2025 is connected to the sample chamber 1012. The top of the bottom plate 2021 is provided with a sealing film (not shown) for sealing the reagent through hole 2024 and the sample injection hole 2025; the upper cover 201 includes an upper plate 2011, the upper plate 2011 has an injection hole 2014 and a sealing hole 2015, a sealing plug 2016 is provided at the sealing hole 2015, and at least one puncture needle 2017 is provided at the bottom of the upper plate 2011; when assembling the box cover 2, the upper cover 201 is screwed together with the lower cover 202.

[0065] During use after assembly is completed, the upper cover 201 has at least a first screwing position, a second screwing position and a third screwing position relative to the lower cover 202. When the upper cover 201 is in the first screwing position, the sealing hole 2015 is opposite to the sample injection hole 2025, and there is a gap between the puncture needle 2017 and the sealing film; when the upper cover 201 is screwed down to the second screwing position relative to the lower cover 202, the sample injection hole 2014 is opposite to the sample injection hole 2025, and the puncture needle 2017 pierces the sealing film at the reagent through-hole 2024; when the upper cover 201 is screwed down to the third screwing position relative to the lower cover 202 or is screwed back to the first screwing position, the sealing hole 2015 is opposite to the sample injection hole 2025, and the sealing plug 2016 arranged at the sealing hole 2015 blocks the sample injection hole 2025.

[0066] In the present invention, the chassis 2021 of the lower cover 202 and the top opening of the box body 1 can be fixedly connected (such as integral injection molding) or detachably connected. Among them, the detachable connection is preferred because it is more convenient for assembly and disassembly and production. However, since the structures of the lower cover 202 and the box body 1 are relatively complex, integral injection molding is difficult in terms of processing technology, prone to quality problems, and will increase production costs. However, molding the lower cover 202 and the box body 1 separately greatly reduces the processing difficulty and improves product quality.

[0067] In the present invention, the reagent chamber 1011 is pre-loaded with reagents required for nucleic acid extraction and amplification. Before the sealing membrane at the reagent through-hole 2024 is punctured, the reagents required for nucleic acid extraction and amplification pre-loaded in the reagent chamber 1011 are in a freeze-dried state; after the sealing membrane at the reagent through-hole 2024 is punctured, the corresponding solvent can be injected into the reagent chamber 1011 to dissolve the freeze-dried reagent into a liquid state.

[0068] The number of reagent chambers 1011 in the present invention can be multiple, and the specific number of reagent chambers 1011 is determined by the reagents required to be stored. Among them, the reagents required for nucleic acid extraction and amplification can be but are not limited to lysis solution, cleaning solution and PCR reaction system.

[0069] Further, such as Figure 3 and Figure 4 As shown, corresponding to the number of reagent chambers 1011, the number of reagent through holes 2024 may also be multiple, and the multiple reagent through holes 2024 correspond one-to-one to the multiple reagent chambers 1011; in addition, there may also be multiple puncture needles 2017 arranged at the bottom of the upper plate 2011. When the upper cover 201 is in the first screwing position, the multiple puncture needles 2017 correspond one-to-one to the multiple reagent through holes 2024, respectively, to ensure that when the upper cover 201 is screwed down, the sealing membranes sealed at each reagent through hole 2024 can be punctured.

[0070] The above technical solution of the present invention is that a sample chamber 1012 for storing a sample to be tested and a reagent chamber 1011 for storing reagents required for nucleic acid extraction and amplification are formed in the box body 1, and a box cover 2 is provided on the top cover of the box body 1. The box cover 2 is divided into an upper and a lower part (i.e., an upper cover 201 and a lower cover 202) that are screwed together. During use, in the initial state, the upper cover 201 is in a first screwing position, at which time the sealing hole 2015 on the upper cover 201 is opposite to the injection hole 2025 on the lower cover 202, and there is a gap between the puncture needle 2017 and the sealing film (the sealing film at the injection hole 2025 is not punctured), which can ensure a stable seal for the reagent chamber 1011; when the upper cover 201 is screwed down to the second screwing position, the injection hole 2014 on the upper cover 201 is rotated to be aligned with the injection hole 2025 on the lower cover 202 The sample holes 2025 are opposite to each other. At this time, the sample injection tool is inserted into the sample injection hole 2014 and punctures the sealing film at the sample injection hole 2025 to inject the sample to be tested into the sample chamber 1012; in the process of screwing the upper cover 201 downward, the puncture needle 2017 at the bottom of the upper cover 201 will puncture the sealing film at the reagent through hole 2024 as it rotates downward, and each reagent chamber 1011 can be connected to the outside world for subsequent nucleic acid extraction and nucleic acid amplification operations; when the upper cover 201 is further screwed down to the third screwing position (of course, the upper cover 201 can also be screwed up to return to the first screwing position), so that the sealing hole 2015 is opposite to the sample injection hole 2025 again, and the sealing plug 2016 can be added to the sealing hole 2015 to seal the sample injection hole 2025. Compared with a hinged lid, the operator only needs to twist the upper cover 201 with one hand to open and close the lid 2 and open the sealing membrane, which is convenient to operate and provides the operator with a better experience. In addition, during the entire operation process, the lid 2 of the present application is always covered on the top of the box body 1, and the opening and closing and opening of the sealing membrane can be completed by twisting the upper cover 201. There will be no leakage due to the lid 2 not being tightly fastened during the opening or closing process, and a good seal is always maintained between the lid 2 and the box body 1.

[0071] In an optional embodiment of the present invention, Figure 3As shown, the top of the chassis 2021 has a coaxially arranged lower outer ring 2022 and a lower inner ring 2023, the radius of the lower outer ring 2022 is larger than the radius of the lower inner ring 2023, the lower outer ring 2022 is arranged on the outer circumference of the lower inner ring 2023, the reagent through hole 2024 is located on the inner side of the lower inner ring 2023, and the sample injection hole 2025 is located between the lower outer ring 2022 and the lower inner ring 2023. The reagent through hole 2024 and the sample injection hole 2025 are separated by the lower outer ring 2022 and the lower inner ring 2023, that is, the reagent through hole 2024 and the sample injection hole 2025 are respectively opposite to the reagent chamber 1011 and the sample chamber 1012 in the box body 1, and can play a certain isolation role when injecting the sample to be tested, thereby avoiding the situation where leakage occurs when adding the sample to be tested and directly enters the reagent chamber 1011.

[0072] Further, such as Figure 4 As shown, the bottom of the upper plate 2011 has an upper outer ring 2012 and an upper inner ring 2013 arranged coaxially, the radius of the upper outer ring 2012 is larger than the radius of the upper inner ring 2013, and the upper outer ring 2012 is arranged on the outer circumference of the upper inner ring 2013; wherein, both the lower outer ring 2022 and the lower inner ring 2023 have a threaded structure (such as: threads are respectively provided on the outer wall of the lower outer ring 2022 and the outer wall of the lower inner ring 2023), and both the upper outer ring 2012 and the upper inner ring 2013 have a threaded structure (such as: thread grooves are respectively provided on the inner wall of the upper outer ring 2012 and the inner wall of the upper inner ring 2013), when the upper cover 201 and the lower cover 202 are screwed together, the lower outer ring 2022 is screwed together with the upper outer ring 2012 through the threaded structure, and the lower inner ring 2023 is screwed together with the upper inner ring 2013 through the threaded structure. The upper cover 201 and the lower cover 202 are connected by two threaded structures to ensure good sealing and avoid leakage due to loose fastening of the box cover 2 during opening or closing.

[0073] In an optional embodiment of the present invention, Figures 1 to 3 As shown, a retaining ring is provided on the periphery of sealing hole 2015. The retaining ring is connected to a cylindrical sealing plug 2016 via an elastic member. This allows sealing plug 2016 to remain connected to upper cover 201 when it is inserted into or removed from sealing hole 2015, facilitating its use and preventing loss. The retaining ring, elastic member, and sealing plug 2016 may be integrally injection molded.

[0074] Furthermore, the sealing plug 2016 is made of a material that is breathable but does not leak liquid. The sealing plug 2016 can be made of, but is not limited to, soft silicone material.

[0075] In an optional embodiment of the present invention, the sealing hole 2015 and the injection hole 2014 have different diameters. In actual use, the upper cover 201 can be rotated to connect the injection hole 2014 with the injection hole 2025, and then the sample to be tested can be injected into the sample chamber 1012 using a sample injection tool. Of course, if the sealing hole 2015 is not provided with a sealing plug 2016, the sealing hole 2015 can also be directly connected to the injection hole 2025, and then the sample to be tested can be injected into the sample chamber 1012 using a sample injection tool, thereby achieving the purpose of adapting to sample injection tools of different sizes. The diameter of the sealing hole 2015 can be set to be larger than the diameter of the injection hole 2014.

[0076] In an optional embodiment of the present invention, Figures 1 to 3 As shown, the upper plate 2011 is provided with a breathable grid 2018. After the puncture needle 2017 pierces the sealing film at the reagent through hole 2024, the reagent chamber 1011 is connected to the outside world through the reagent through hole 2024 and the breathable grid 2018. During the nucleic acid extraction and nucleic acid amplification process, the reagent chamber 1011 needs to be connected to the outside world to ensure that when the reagent in each reagent chamber 1011 is used, the corresponding reagent chamber 1011 can change the required pressure according to the use of the reagent, so as to avoid the reagent being unable to be taken out in a completely sealed state.

[0077] In an optional embodiment of the present invention, Figures 3 to 5As shown, among the multiple accommodating cavities 101, at least one accommodating cavity 101 is configured as a plunger cavity 1013, and the plunger cavity 1013 is located in the middle position of the box body 1, and the sample cavity 1012 and the multiple reagent cavities 1011 are arranged around the periphery of the plunger cavity 1013; a plunger 2019 is provided at the bottom of the upper plate 2011 and is coaxially arranged with the upper outer ring 2012 and the upper inner ring 2013, and the radius of the upper inner ring 2013 is greater than the radius of the plunger 2019, and the plunger 2019 is located on the inner side of the upper inner ring 2013; a plunger hole 2026 is provided in the middle position of the bottom plate 2021 and is coaxially arranged with the lower outer ring 2022 and the lower inner ring 2023, and the plunger hole 2026 is communicated with the plunger cavity 1013, and when the upper cover 201 and the lower cover 202 are assembled, the plunger 2019 is arranged coaxially with the upper outer ring 2022 and the lower inner ring 2023. The plug 2019 can be movably inserted into the plunger hole 2026, and the outer wall of the plunger 2019 is sealedly connected to the inner wall of the plunger hole 2026, wherein the plunger hole 2026 and the plunger 2019 are both hollow cylinders. After the plunger hole 2026 is sealedly connected to the plunger 2019, an external device (such as a driving member that can drive the soft rubber plug 307 to move up and down) is connected to the plunger cavity 1013, and the soft rubber plug 307 on the driving member can pass through the plunger hole 2026 and the plunger 2019 into the plunger cavity 1013. The outer wall of the soft rubber plug 307 is slidably sealed with the inner wall of the plunger cavity 1013. The driving member can move up and down along the plunger cavity 1013 to drive the soft rubber plug 307 thereon to move up and down, so as to achieve the purpose of adjusting the pressure in the plunger cavity 1013. Among them, Figure 7 As shown, the driving member may be but is not limited to a driving rod 308 , and the soft rubber plug 307 is provided at one end of the driving rod 308 , and the driving rod 308 can be driven to perform telescopic movement by an external device.

[0078] Further, such as Figure 3 As shown, an annular boss 2027 is provided on the inner side of the lower inner ring 2023 and on the outer periphery of the plunger hole 2026, and the reagent through hole 2024 is arranged on the annular boss 2027; a sealing membrane for sealing the reagent through hole 2024 is arranged on the top surface of the annular boss 2027, thereby providing a setting position for the sealing membrane.

[0079] In an optional embodiment of the present invention, Figures 5 to 7As shown, the bottom wall of the box body 1 is provided with a plurality of liquid holes 102, and the plurality of liquid holes 102 correspond to and are connected with the plurality of accommodating chambers 101 one by one; the bottom of the box body 1 is provided with a flow channel switching valve 3 which can control its rotation, and the flow channel switching valve 3 has a transfer storage chamber 301, and the transfer storage chamber 301 is connected with the plunger chamber 1013, and the flow channel switching valve 3 is provided with a first inlet and outlet 302 and a second inlet and outlet 303 which are connected with the transfer storage chamber 301, and the flow channel switching valve 3 can be driven to rotate around the axis of the plunger chamber 1013 so that one of the first inlet and outlet 302 and the second inlet and outlet 303 is connected with one of the plurality of liquid holes 102. When a flow channel switching valve 3 is provided, the soft rubber plug 307 on the driving member can pass through the plunger hole 2026 and the plunger 2019 to reach the transfer storage chamber 301. The driving member can move up and down along the transfer storage chamber 301 to drive the connected soft rubber plug 307 to move up and down, so as to achieve the purpose of adjusting the pressure in the transfer storage chamber 301.

[0080] The use process of the reagent container for nucleic acid extraction and nucleic acid amplification of the present invention is as follows:

[0081] like Figure 1 As shown, the state of the reagent container of the present invention when it is not in use is shown. Figure 4 As shown in FIG, the state of the reagent container of the present invention after the sample injection operation is completed. Figure 1As shown, at this time, the upper cover 201 and the lower cover 202 are in a loose connection state (that is, the upper cover 201 is in the first screwing position), and the reagent through hole 2024 and the sample injection hole 2025 are sealed by the sealing film to prevent the reagent container from leaking during transportation or transfer. At this time, the lower cover 202 and the box body 1 are in a connected state, and the sealing plug 2016 does not seal the sealing hole 2015. After the user takes the reagent container in this state, he can screw the upper cover 201 with one hand, so that the upper cover 201 rotates and moves downward until it is rotated to the point where the sample injection hole 2014 is located directly above the sample injection hole 2025 (the two are vertically opposite to each other). At this time, the screwing of the upper cover 201 is paused ( The user can then use the injection tool with his other hand to perform the injection operation, puncturing the sealing membrane at the injection hole 2025 with the injection tool and injecting the sample to be tested into the sample chamber 1012. The user can then continue to screw the upper cover 201 in the original direction to the third screwing position or reversely screw the upper cover 201 to return to the first screwing position, so that the sealing hole 2015 is again directly above the injection hole 2025. At this point, the upper cover 201 and the lower cover 202 are in a tightly connected state. The sealing plug 2016 can then be inserted into the sealing hole 2015, and at least part of the sealing plug 2016 blocks the injection hole 2025 to prevent leakage of the sample to be tested. During the process of screwing the upper cover 201 downward, the sealing membrane on the annular boss 2027 is punctured and cut open by the puncture needle 2017, thereby allowing the sealed reagent chamber 1011 to communicate with the outside world. After the sample injection operation is completed, the reagent container of the present invention can be placed on an instrument to perform nucleic acid extraction and nucleic acid amplification procedures.

[0082] In addition, it should be noted that the operation process of the reagent container is not limited to the above-mentioned content, but can also be: after the user gets the reagent container (such as Figure 1 The sealing film at the injection hole 2025 is punctured directly through the sample injection tool through the sealing hole 2015, and the sample to be tested is injected into the sample chamber 1012 through the sample injection tool. Then, the upper cover 201 is twisted for one circle so that the sealing hole 2015 is again located directly above the injection hole 2025, and the sealing hole 2015 and the injection hole 2025 are sealed with the sealing plug 2016. At this time, the upper cover 201 is tightly connected to the lower cover 202. In the process of twisting the upper cover 201, the sealing film on the annular boss 2027 will be punctured and cut open by the puncture needle 2017, so that the sealed reagent chamber 1011 can be connected to the outside world.

[0083] The following describes the corresponding structure of the flow channel switching valve 3 in the reagent container for nucleic acid extraction and nucleic acid amplification of the present invention and the principle of pipetting operation: Figures 5 to 7As shown, the multiple accommodating chambers 101 include a plunger chamber 1013, a sample chamber 1012 and multiple reagent chambers 1011, wherein the multiple reagent chambers 1011 can be, but are not limited to, a cleaning liquid chamber, a buffer chamber, a waste liquid chamber, a first reserved chamber (i.e., for subsequent function expansion), a second reserved chamber, etc., and the bottom wall of each chamber has a corresponding liquid hole 102 (such as Figure 6 As shown), it can be understood that the channel switching valve 3 can be driven to rotate by a preset angle through a corresponding control driving component (such as a drive motor, the output shaft of the drive motor is connected to the body of the channel switching valve 3), so that one of the first inlet and outlet 302 and the second inlet and outlet 303 on the channel switching valve 3 is connected to one of the aforementioned multiple reagent chambers 1011 through the liquid hole 102 thereon, and then the external device connected to the transfer storage chamber 301 moves up and down along the transfer storage chamber 301 to drive the connected soft rubber plug 307 to move up and down, so as to achieve the purpose of adjusting the pressure in the transfer storage chamber 301, thereby changing the pressure in the transfer storage chamber 301 to suck the liquid reagent located in the reagent chamber 1011 into the transfer storage chamber 301 of the channel switching valve 3 through the liquid hole 102, or transfer it from the transfer storage chamber 301 of the channel switching valve 3 to the corresponding reagent chamber 1011. It can be seen that the channel switching valve 3 realizes the function of the pipette gun in the prior art.

[0084] In a specific embodiment of the present invention, Figures 5 to 7 As shown, the flow channel switching valve 3 includes a cylinder 304 inserted in the plunger cavity 1013 and a valve seat 305 at the bottom end of the cylinder 304. The soft rubber plug 307 connected to the aforementioned external device can be slidably arranged in the cylinder cavity of the cylinder 304 (that is, the transfer storage cavity 301). The first inlet and outlet 302 and the second inlet and outlet 303 are located on the valve seat 305. The external device connected to the transfer storage cavity 301 moves up and down along the transfer storage cavity 301 to drive the connected soft rubber plug 307 to move up and down, so as to change the size of the transfer storage cavity 301, thereby realizing the liquid inlet or outlet control of the transfer storage cavity 301.

[0085] Further, such as Figure 7 As shown, an ultrasonic chamber 306 is also formed within the valve seat 305. The ultrasonic chamber 306 is in communication with the first inlet and outlet 302, the second inlet and outlet 303, and the transfer storage chamber 301. An ultrasonic generator is provided at the bottom of the ultrasonic chamber 306. The ultrasonic waves emitted by the ultrasonic generator achieve a nucleic acid lysis function, thereby ultrasonically lysing the sample to be tested and releasing the nucleic acid to be detected.

[0086] The characteristics and advantages of the reagent container for nucleic acid extraction and nucleic acid amplification of the present invention are:

[0087] 1. The reagent container for nucleic acid extraction and nucleic acid amplification changes the existing hinged connection between the box cover and the box body into a screw connection. On the one hand, it allows the user to operate with one hand, saving time and effort and improving the injection efficiency. On the other hand, it simplifies the operation steps. The user only needs to twist the upper cover 201 with one hand and perform the sample injection operation with the other hand. There is no need to apply excessive force with both hands to open the cover body and then perform the sample injection (after injection, both hands are still needed to cooperate to tighten the box cover and the box body), and there is no need to manually tear off the film.

[0088] 2. During use of the reagent container for nucleic acid extraction and nucleic acid amplification, the box cover 2 is always covered on the top of the box body 1. Operations such as opening and closing and opening of the sealing film can be completed by screwing the upper cover 201. There will be no leakage due to loose fastening of the box cover 2 during opening or closing, and a good seal is always maintained between the box cover 2 and the box body 1.

[0089] 3. The reagent container for nucleic acid extraction and nucleic acid amplification can be used with injection tools of different sizes, and has stronger compatibility and wider applicability.

[0090] It should be noted that, in the description of this application, the terms "first," "second," etc., are used solely for descriptive purposes and to distinguish similar objects. There is no order of precedence between the two, nor should they be understood to indicate or imply relative importance. Furthermore, in the description of this application, unless otherwise specified, "plurality" means two or more.

[0091] The above-mentioned various embodiments in this specification are described in a progressive manner. The same or similar parts between the various embodiments can be referenced to each other. Each embodiment focuses on the differences from other embodiments.

[0092] The above are only a few embodiments of the present invention. Although the embodiments disclosed in the present invention are as above, the contents are only for the purpose of facilitating understanding of the present invention and are not intended to limit the present invention. Any equivalent changes and modifications made by any person skilled in the art without departing from the concept and principle of the present invention shall fall within the scope of protection of the present invention.

Claims

1. A reagent container for nucleic acid extraction and nucleic acid amplification, characterized in that: include: A box body having a plurality of accommodating chambers with an open top and a sealed bottom, wherein the plurality of accommodating chambers are respectively configured as a sample chamber for storing samples to be tested and a reagent chamber for storing reagents required for nucleic acid extraction and amplification; The box cover comprises an upper cover and a lower cover, wherein: The lower cover includes a base plate, which is arranged at the top opening of the box body through the base plate. The base plate has a reagent through hole and a sample injection hole, the reagent through hole is connected to the reagent cavity, and the sample injection hole is connected to the sample cavity. A sealing film is provided on the top of the base plate to seal the reagent through hole and the sample injection hole; The upper cover comprises an upper plate, the upper plate having an injection hole and a sealing hole, a sealing plug is provided at the sealing hole, and at least one puncture needle is provided at the bottom of the upper plate; The upper cover is screwed together with the lower cover, and the upper cover has at least a first screwing position, a second screwing position, and a third screwing position relative to the lower cover. When the upper cover is in the first screwing position, the sealing hole is opposite to the injection hole, and a gap is formed between the puncture needle and the sealing film; when the upper cover is screwed down to the second screwing position, the injection hole is opposite to the injection hole, and the puncture needle punctures the sealing film at the reagent through-hole; when the upper cover is screwed down to the third screwing position or is screwed back to the first screwing position, the sealing hole is opposite to the injection hole, and the sealing plug arranged at the sealing hole blocks the injection hole.

2. The reagent container for nucleic acid extraction and nucleic acid amplification according to claim 1, wherein The top of the chassis has a lower outer ring and a lower inner ring arranged coaxially, the lower outer ring is arranged on the outer circumference of the lower inner ring, the reagent through hole is located on the inner side of the lower inner ring, and the sample injection hole is located between the lower outer ring and the lower inner ring; The lower outer ring and the lower inner ring both have thread structures.

3. The reagent container for nucleic acid extraction and nucleic acid amplification according to claim 1 or 2, characterized in that: There are multiple reagent through holes, and the multiple reagent through holes correspond one-to-one to the multiple reagent cavities.

4. The reagent container for nucleic acid extraction and nucleic acid amplification according to claim 2, wherein: The bottom of the upper plate has an upper outer ring and an upper inner ring arranged coaxially, and the upper outer ring is arranged on the outer circumference of the upper inner ring; The upper outer ring and the upper inner ring both have a threaded structure. When the upper cover and the lower cover are screwed together, the lower outer ring and the upper outer ring are screwed together via the threaded structure, and the lower inner ring and the upper inner ring are screwed together via the threaded structure.

5. The reagent container for nucleic acid extraction and nucleic acid amplification according to claim 1, wherein The sealing hole and the injection hole have different diameters, and are connected to the injection hole through the sealing hole or the injection hole to adapt to injection tools of different sizes.

6. The reagent container for nucleic acid extraction and nucleic acid amplification according to claim 1, wherein The upper plate is provided with a breathable grid. After the puncture needle punctures the sealing film at the reagent through hole, the reagent cavity is connected to the outside world through the reagent through hole and the breathable grid opposite thereto in sequence.

7. The reagent container for nucleic acid extraction and nucleic acid amplification according to claim 1, wherein When the upper cover is screwed downward relative to the lower cover to the second screwing position, a sample injection tool is inserted through the injection hole and punctures the sealing film at the injection hole to inject the sample to be tested into the sample cavity.

8. The reagent container for nucleic acid extraction and nucleic acid amplification according to claim 4, wherein: At least one of the accommodating cavities is configured as a plunger cavity, and the sample cavity and the reagent cavity are both arranged around the periphery of the plunger cavity; The bottom of the upper plate has a plunger coaxially arranged with the upper outer ring and the upper inner ring, and the plunger is located on the inner side of the upper inner ring; The chassis is provided with a plunger hole coaxially arranged with the lower outer ring and the lower inner ring. The plunger hole is communicated with the plunger cavity, and the plunger can be movably inserted into the plunger hole.

9. The reagent container for nucleic acid extraction and nucleic acid amplification according to claim 8, wherein An annular boss is provided on the inner side of the lower inner ring and on the outer periphery of the plunger hole, and the reagent through hole is provided on the annular boss; The sealing film for sealing the reagent through hole is arranged on the top surface of the annular boss.

10. The reagent container for nucleic acid extraction and nucleic acid amplification according to claim 8, wherein The bottom wall of the box body is provided with a plurality of liquid holes, and the plurality of liquid holes correspond to and communicate with the plurality of accommodating cavities one by one; A flow channel switching valve is provided at the bottom of the box body, and the flow channel switching valve has a transfer storage chamber, and the transfer storage chamber is communicated with the plunger chamber. The flow channel switching valve is provided with a first inlet and a second inlet and a second inlet that are communicated with the transfer storage chamber. The flow channel switching valve can be driven to rotate around the axis of the plunger chamber so that one of the first inlet and the second inlet is communicated with one of the multiple liquid holes.

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