Livestock and poultry breeding wastewater treatment device based on dynamic bacterial-algae biological membrane and method and application of livestock and poultry breeding wastewater treatment device

Abstract

The invention discloses a livestock and poultry breeding wastewater treatment device based on a dynamic bacterial-algae biological membrane and a method and application thereof, and relates to the field of livestock and poultry breeding tail water treatment. According to the biological treatment membrane bed unit, biological treatment and evaporation processes are creatively combined, and the system stability and the wastewater purification efficiency are remarkably improved. The unit is high in environmental adaptability and free of emission and peculiar smell during operation, the wastewater volume is effectively reduced, and the limitation of resource utilization of aquaculture wastewater is broken through. The biological membrane contains a plurality of microorganisms, including high-efficiency microorganisms added at the early stage and microorganisms captured from the environment, so that the operation and maintenance cost of equipment is reduced. Microalgae cells grow in the form of a biological membrane and are easy and convenient to harvest, and automatic continuous production is realized. According to the technology, organic waste in the breeding wastewater is recycled, the product is widely applied to multiple fields, the biological carbon sequestration function is achieved, and the carbon trapping device is low in cost, high in output value, green and environmentally friendly and assists in constructing a sustainable technical system and a circular economy mode.

Description

Technical Field

[0001] The present invention relates to the field of livestock and poultry breeding tail water treatment, and in particular to a livestock and poultry breeding wastewater treatment device based on dynamic bacteria and algae biofilm, and a method and application thereof. Background Art

[0002] With the rapid expansion of livestock and poultry farming, the environmental problems it causes are becoming increasingly serious. Livestock and poultry manure remains the main source of agricultural pollution. Specifically, (1) water-soluble organic matter, pathogenic microorganisms, nitrogen, phosphorus and other substances in livestock and poultry manure enter water bodies, causing eutrophication, triggering algal blooms, damaging aquatic ecosystems, and threatening the health of surrounding residents; (2) manure discharge exceeds the self-purification capacity of the soil, leading to soil salinization, compaction, decreased aeration, nutrient imbalance, and even heavy metal pollution of the soil due to trace elements in the feed; (3) microbial decomposition in manure produces malodorous gases such as NH3 and H2S, which reduce the resistance of livestock and poultry on farms and have a negative impact on the living environment of surrounding residents.

[0003] Biogas projects are one of the main energy-based approaches for treating livestock and poultry manure at home and abroad. Through the action of anaerobic and facultative anaerobic microorganisms, the organic matter in manure is converted into biogas, biogas residue and biogas liquid. Biogas liquid is rich in organic elements such as nitrogen, phosphorus and potassium, as well as bioactive substances, and is suitable for plant growth. However, the output of biogas liquid is huge. The pig farming industry alone produces up to 9 million cubic meters of biogas liquid per day. At present, the main way to utilize biogas liquid is to combine farming and breeding, but this requires large areas of arable land, making it difficult to effectively utilize a large amount of biogas liquid. It usually needs to be further processed in high-cost A / O tanks. There is no large-scale biogas liquid resource technology that can be promoted on a large scale.

[0004] Microalgae are a type of photosynthetic microorganism widely distributed in natural water bodies and are widely used in livestock and poultry farming, aquaculture, biofuels, healthcare, and biomaterials. Currently, large-scale commercial microalgae cultivation relies on the addition of nutrients such as fertilizers and organic matter. This is not only costly and complex, but also results in secondary pollution from organic wastewater at the end of production. The rich nutrients in aquaculture wastewater offer potential for commercial microalgae cultivation. Research has shown that biogas slurry after anaerobic treatment in biogas digesters contains high levels of free ammonia and a carbon-nitrogen ratio typically below 6, making it unsuitable for direct microalgae cultivation, potentially leading to slow algae growth or even death. By further biochemical treatment of the biogas slurry and adjusting its composition, it can be converted into a high-concentration culture medium suitable for microalgae growth. The nutrients in the wastewater can be used to produce microalgae liquid and powder on a large scale, and then to extract bioenergy, bioactive substances, and biomaterials, thereby realizing the resource utilization and high-value utilization of aquaculture wastewater.

[0005] Therefore, it is of great practical significance to develop a water treatment equipment that can simultaneously process large amounts of biogas slurry and produce high-quality microalgae culture medium. Summary of the Invention

[0006] In response to the shortcomings of the existing technology, the present invention discloses a livestock and poultry breeding wastewater treatment device based on dynamic bacterial and algal biofilm and its application. Through the use of the device, a water treatment equipment is realized that can simultaneously process large amounts of biogas slurry and produce high-quality microalgae culture medium. At the same time, it has the characteristics of high purification efficiency, no emissions, and no odor, reduces the volume of wastewater, and breaks through the limitations of resource utilization of breeding wastewater.

[0007] To achieve the above-mentioned purpose, the present application discloses a livestock and poultry breeding wastewater treatment system based on dynamic bacteria-algae biofilm technology, which consists of a biological treatment membrane bed unit, a water tank, a biological filter material layer and a water-air pumping system; the biological treatment membrane bed unit is fixed on the top of the water tank, and a plurality of groups of suspended biofilm beds are arranged inside it; the water tank is divided into three functional areas, an aeration tank, an intermediate sedimentation tank and a conversion tank by a wall, and the breeding wastewater to be treated is stored in the tank; the intermediate sedimentation tank is equipped with biological treatment materials such as Widmannian Bacillus bacteria bags, Escherichia coli bags and microalgae seed liquid; the biological filter material layer is arranged on the top of the water tank, and a gauze cushion layer is laid on the bottom; the wind screen in the biological treatment membrane bed unit is made of one or more of polyester, nylon or other chemical fiber materials, and is black or dark gray in color, and has a coil or thread array rough surface made by wire drawing process to assist biofilm formation, the wind screen hanging density is 7-10 sheets / m, and the total working area of a single biofilm bed is 12-20m 2 .

[0008] Furthermore, the water vapor pumping system includes a spray pipe and a spray pump, one end of the spray pipe is connected to the lower end of the water tank, and a spray device is arranged at one end of the spray pipe extending to the interior of the biological treatment membrane bed unit. A comprehensive treatment unit is arranged on one side of the water tank, and a negative pressure fractionator is installed inside the comprehensive treatment unit. One side of the negative pressure fractionator is connected to a moisture absorption pipe communicating with the conversion tank, and the top of the negative pressure fractionator is connected to an external discharge pipe extending to the outside of the comprehensive treatment unit. An aeration pump is installed inside the comprehensive treatment unit, and an aeration pipe connected to the aeration pump is arranged inside the aeration tank, and an aeration head is arranged at the top of the aeration pipe. The aeration tank is connected to the aquaculture wastewater tank through a water supply pipe and a water supply pump. One side of the water tank is connected to the runway tank through a liquid delivery pipe, and a photobioreactor is installed at one end of the runway tank.

[0009] Furthermore, the distribution density of the spraying devices is 7-10 pieces / m, and the flow rate of a single spraying device is set to 150-250 L / h.

[0010] Furthermore, the biological filter material layer is composed of rice husks or other corrosion-resistant plant-based biological materials, with a thickness of 150-350 mm, and the mesh size of the underlying gauze cushion layer is 50-100 meshes.

[0011] Furthermore, the distribution density of the aeration heads is 2 / m, and the gas flow rate of a single aeration head is 2000-5000 L / h.

[0012] Furthermore, the flow rate of the water supply pipe is set to 10-15m 3 / h.

[0013] Furthermore, the application specifications of the Widmannian Bacillus bacteria bag and the Marmota Escherichia coli bag are 10 8 -10 10 cfu / g.

[0014] Furthermore, the microalgae seed liquid includes but is not limited to one or more of Chlorella, Chlamydomonas, diatoms, and Anabaena.

[0015] The present invention also discloses a method for treating livestock and poultry breeding wastewater based on a dynamic bacterial and algal biofilm, the method comprising the following steps: S1. Wastewater inlet and flow: Aquaculture wastewater that has undergone anaerobic fermentation or other pretreatment is introduced into the aeration tank, and the wastewater flows in the multi-stage tank of the water tank in an overflow manner.

[0016] S2. Mixing and Spraying: After the wastewater is mixed with the bacterial bags and microalgae seed solution in the intermediate settling tank, it is evenly sprayed onto the vertical biological treatment membrane bed unit through a spray pipe, distributing the bacteria and microalgae on the wind screen. The wastewater then flows back to the water tank, continuing the cycle.

[0017] S3. Biofilm Formation: After 1-5 days, a stable biofilm, 2-5mm thick, is formed by microorganisms trapped in the bacterial bags, microalgae seed solution, and air. The biofilm composition automatically adjusts to regional, temperature, and light conditions.

[0018] S4. Wastewater treatment and concentration: The wastewater is concentrated to 10%-30% of its original volume after natural evaporation with a hydraulic retention time of 3-15 days and a circulation rate of 7-15 m³ / h.

[0019] S5. Biofilm cleaning and utilization: Partially clean the vertical biotreatment membrane bed unit every 6-8 weeks, retaining 25%-50% of the original biofilm. The cleaned biofilm can be used to extract bioactive components or natural biological materials.

[0020] S6. Utilization of concentrated tail water: The treated concentrated tail water is used as a high-efficiency microalgae culture medium for subsequent large-scale microalgae cultivation. It can be connected to circular pools, runway pools, bag-type photoreactors and other equipment for continuous cultivation.

[0021] At the same time, the present invention also discloses the application of an aquaculture wastewater treatment device based on a dynamic bacterial and algal biofilm, which is characterized in that the equipment is used for aquaculture wastewater treatment, the obtained concentrated tail water can be used for large-scale cultivation of microalgae, and the obtained biofilm can be used to extract biologically active substances or natural biological materials.

[0022] Beneficial effects of the technical solution of the present invention 1. The biotreatment membrane bed unit of this invention innovatively combines biological treatment with evaporation processes, offering numerous significant advantages. The high biofilm capacity ratio and biomass density per unit area significantly enhance system stability and significantly improve wastewater purification efficiency. Furthermore, the membrane bed unit exhibits excellent environmental adaptability, with zero emissions and no odor during operation. While converting and consuming organic waste in the water, it also effectively reduces the total volume of wastewater, successfully overcoming the limitations and obstacles previously faced in the resource utilization of aquaculture wastewater.

[0023] 2. The biofilm of the biotreatment membrane bed unit of this invention contains a diverse array of microorganisms. In addition to several highly effective microorganisms artificially added during the initial operation of the equipment, microorganisms are captured from the natural environment through air flow and naturally screened within the biotreatment membrane bed, forming a unique local microbiome. This microbiome is highly resilient to environmental changes, and its biological composition can self-adjust based on local environmental conditions such as temperature, humidity, and light, ensuring stable operation of the wastewater treatment equipment. This characteristic also reduces commissioning, operation, and maintenance costs under varying conditions.

[0024] 3. The microalgae cells in the biofilm of the biotreatment membrane bed unit of the present invention grow and reproduce as a biofilm attached to the surface of a solid substrate. Harvesting can be accomplished through simple hydraulic flushing and scraping, which is technically simple and significantly reduces costs compared to traditional harvesting techniques. Furthermore, the membrane bed unit can be connected to external microalgae cultivation equipment, leveraging the natural shedding of the biofilm to naturally inoculate subsequent cultures, enabling automated, continuous, and large-scale production of microalgae, thereby reducing labor costs and worker intensity.

[0025] 4. This invention achieves resource utilization of organic waste in aquaculture wastewater treatment. The resulting product has a wide range of applications, including livestock and poultry farming, aquaculture, biofuels, healthcare, biomaterials, and other fields. Furthermore, the device also possesses biological carbon sequestration capabilities, making it a low-cost, high-value, and environmentally friendly new carbon capture device. This not only contributes to the development of a sustainable technology system for energy conservation, emission reduction, and efficient waste resource utilization, but also extends the industrial chain of livestock and poultry farming enterprises, contributing to the creation of a new circular economy model. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. Those skilled in the art can also derive other drawings based on these drawings without inventive effort.

[0027] Figure 1 Schematic diagram of the structure of the system of the present invention; Figure 2 for Figure 1 A-direction structural diagram; Figure 3 This is a physical picture of the system of the present invention being used for livestock and poultry breeding wastewater treatment; Figure 4 a microalgae culture medium produced by the system of the present invention; Figure 5 Microscopic morphology of microalgae cultured in the microalgae culture medium produced by the system of the present invention Figure 1 .

[0028] Figure 6 Microscopic morphology of microalgae cultured in the microalgae culture medium produced by the system of the present invention Figure 2 .

[0029] In the figure, 1. biological treatment membrane bed unit; 2. biological membrane bed; 3. spraying device; 4. spraying pipe; 5. spraying pump; 6. integrated treatment unit; 7. moisture absorption pipe; 8. negative pressure fractionator; 9. photobioreactor; 10. runway pool; 11. water supply pipe; 12. water supply pump; 13. aeration pump; 14. aeration tank; 15. liquid delivery pipe; 16. intermediate sedimentation tank; 17. Widmannian Bacillus bag; 18. aquaculture wastewater; 19. Marmota Escherichia bag; 20. wall; 21. aeration head; 22. aeration pipe; 23. wastewater inlet pipe; 24. water tank; 25. biological filter layer; 26. external discharge pipe; 27. conversion tank. DETAILED DESCRIPTION

[0030] In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they cannot be understood as limiting the present invention.

[0031] In the description of the present invention, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they may refer to fixed, detachable, or integral connections; mechanical or electrical connections; direct or indirect connections through an intermediate medium; and internal connections between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on the specific circumstances.

[0032] The following describes the implementation of the present invention using specific embodiments. Those skilled in the art will readily understand the other advantages and benefits of the present invention from the disclosure herein. Obviously, the embodiments described are only a portion of the present invention, not all of it. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without inventive effort are intended to fall within the scope of protection of the present invention.

[0033] refer to Figure 1-3 A livestock and poultry breeding wastewater treatment system based on dynamic bacteria and algae biofilm technology consists of a biological treatment membrane bed unit 1, a water tank 24, a biological filter layer 25 and a water-air pumping system; wherein the biological treatment membrane bed unit 1 is fixed on the top of the water tank 24, and multiple groups of suspended biofilm beds 2 are arranged inside it; the water tank 24 is divided into three functional areas by a wall 20: an aeration tank 14, a middle sedimentation tank 16 and a conversion tank 27, wherein the aquaculture wastewater 18 to be treated is stored in the tank; the middle sedimentation tank 16 is equipped with a bacillus spore bag of Widmannii 17. Biotreatment materials such as Escherichia coli package 19 and microalgae seed liquid; a biological filter material layer 25 is arranged on the top of the water tank 24, and a gauze cushion layer is laid on the bottom; the wind screen in the biological treatment membrane bed unit 1 is made of one or more of polyester, nylon or other chemical fiber materials, and is black or dark gray in color. It has a rough surface of coils or thread arrays made by wire drawing process to assist biofilm formation. The wind screen hanging density is 7-10 sheets / m, and the total working area of a single biofilm bed 2 is 12-20m 2 .

[0034] In addition, the water vapor pumping system includes a spray pipe 4 and a spray pump 5. One end of the spray pipe 4 is connected to the lower end of the water tank 24. The spray pipe 4 extends to one end of the biological treatment membrane bed unit 1 and is spaced apart with a spray device 3. One side of the water tank 24 is provided with a comprehensive treatment unit 6. A negative pressure fractionator 8 is installed inside the comprehensive treatment unit 6. One side of the negative pressure fractionator 8 is connected to a moisture absorption pipe 7 communicated with the conversion tank 27. The top of the negative pressure fractionator 8 is connected to an extension pipe. An external discharge pipe 26 extends to the outside of the integrated treatment unit 6, an aeration pump 13 is installed inside the integrated treatment unit 6, an aeration pipe 22 connected to the aeration pump 13 is provided inside the aeration tank 14, and an aeration head 21 is provided at the top of the aeration pipe 22, the aeration tank 14 is connected to the aquaculture wastewater tank through a water supply pipe 11 and a water supply pump 12, one side of the water tank pool 24 is connected to the runway pool 10 through a liquid delivery pipe 15, and a photobioreactor 9 is installed at one end of the runway pool 10.

[0035] In this embodiment, the function of the biological treatment membrane bed unit 1 is as follows: The biological treatment membrane bed unit is the core processing unit of the system, and multiple groups of biofilm beds 2 are suspended inside to cultivate and fix microorganisms and microalgae to form biofilms. The biofilm achieves the purpose of purifying water quality by adsorbing, degrading and transforming pollutants such as organic matter, nitrogen and phosphorus in wastewater. The wind screen on the biofilm bed is made of chemical fiber materials such as polyester and nylon. The surface has a rough structure formed by a wire drawing process, which facilitates the attachment and growth of microorganisms and microalgae. The hanging density of the wind screen is 7-10 sheets / m, ensuring sufficient surface area for biofilm formation.

[0036] Function of the water tank 24: The water tank is the primary container for wastewater treatment and is divided into three functional areas: an aeration tank 14, an intermediate settling tank 16, and a conversion tank 27, respectively used for aeration, sedimentation, and further treatment of the wastewater. Aeration tank 14: Air is injected into the wastewater via an aeration pump 13 and aeration pipe 22, providing oxygen to promote the growth and metabolism of aerobic microorganisms and accelerate the degradation of organic matter. Intermediate settling tank 16: Used to precipitate suspended matter and microbial flocs in the wastewater. It is equipped with biological treatment materials such as Widmannian Bacillus bacteria bags 17, Escherichia coli bags 19, and microalgae seed liquid to further degrade organic matter and remove nutrients such as nitrogen and phosphorus. Conversion tank 27: Used for further treatment and conversion of the wastewater, volatile organic compounds and gases are removed from the wastewater via a negative pressure fractionator 8 and a moisture absorption pipe 7.

[0037] The biological filter layer 25, located at the top of the tank and with a mesh underlayment at the bottom, filters suspended matter and microorganisms from the wastewater, while also providing a surface for microorganisms to attach, further purifying the water. As wastewater passes through the biological filter layer, suspended matter is trapped, and microorganisms form a biofilm on the filter surface, further degrading organic matter and removing pollutants such as nitrogen and phosphorus.

[0038] The spray pipe 4 and spray pump 5 of the water-gas pumping system are used to connect one end of the spray pipe to the water tank and the other end to the interior of the biological treatment membrane bed unit. The wastewater is evenly sprayed onto the biofilm bed through the spray device 3 to ensure full contact between the wastewater and the biofilm, thereby promoting the degradation of pollutants. Aeration pump 13 and aeration pipe 22: The aeration pump injects air into the wastewater in the aeration tank through the aeration pipe, providing oxygen to promote the growth and metabolism of aerobic microorganisms and accelerate the degradation of organic matter. Negative pressure fractionator 8 and moisture absorption pipe 7: The negative pressure fractionator extracts volatile organic compounds and gases from the conversion tank through the moisture absorption pipe, and after treatment, discharges them from the system through the external exhaust pipe 26, thereby reducing waste gas emissions.

[0039] Function of the integrated treatment unit 6: This unit centrally controls the operation of the system. It houses a negative pressure fractionator 8, an aeration pump 13, and other equipment to ensure coordinated operation of all system components. The negative pressure fractionator processes volatile organic compounds and gases, while the aeration pump controls the oxygen supply in the aeration tank, ensuring normal microbial growth and metabolism.

[0040] The raceway pool 10 and photobioreactor 9 are used to further treat wastewater. The photobioreactor uses light to promote the growth of microalgae, further removing nutrients such as nitrogen and phosphorus from the wastewater. Wastewater enters the raceway pool through liquid delivery pipe 15. The microalgae in the photobioreactor undergo photosynthesis under the illumination, absorbing nutrients such as nitrogen and phosphorus from the wastewater while releasing oxygen, further purifying the water.

[0041] When in use, wastewater enters the water tank: the aquaculture wastewater first enters the aeration tank 14 of the water tank, and is aerated through the aeration pump 13 and the aeration pipe 22 to provide oxygen to promote the growth and metabolism of aerobic microorganisms.

[0042] Biofilm treatment: wastewater is evenly sprayed onto the biotreatment membrane bed unit 1 through the spray pipe 4 and the spray pump 5. The microorganisms and microalgae on the biofilm bed adsorb and degrade organic matter and nutrients in the wastewater.

[0043] Sedimentation and further treatment: The wastewater enters the intermediate sedimentation tank 16, where suspended solids and microbial flocs are precipitated. The configured bacterial bags and microalgae seed liquid further degrade organic matter and remove nitrogen and phosphorus.

[0044] Conversion and waste gas treatment: The wastewater enters the conversion tank 27 and passes through the negative pressure fractionator 8 and the moisture absorption pipe 7 to remove volatile organic compounds and gases, thereby reducing waste gas emissions.

[0045] Filtration and photobiological reaction: The wastewater is filtered through the biological filter layer 25 and then enters the runway pool 10. The microalgae in the photobioreactor 9 further remove nutrients such as nitrogen and phosphorus under light, ultimately achieving wastewater purification.

[0046] On the basis of the above embodiment, the distribution density of the spray device 3 is 7-10 pieces / m, and the flow rate of a single spray device 3 is set to 150-250L / h. The biological filter layer 25 is composed of rice husks or other corrosion-resistant plant-based biological materials, with a thickness of 150-350mm, and the mesh size of the mesh pad below is 50-100 mesh. The distribution density of the aeration head 21 is 2 pieces / m, and the gas flow rate of a single head of the aeration head 21 is 2000-5000L / h. The flow rate of the water supply pipe 11 is set to 10-15m 3 / h. The application specifications of the Widmannian Bacillus bacteria bag 17 and the Marmota Escherichia coli bag 19 are 10 8 -10 10 The microalgae seed liquid includes, but is not limited to, one or more of Chlorella, Chlamydomonas, diatoms, and Anabaena.

[0047] The present invention also discloses a method for treating livestock and poultry breeding wastewater based on a dynamic bacterial and algal biofilm, the method comprising the following steps: S1. Wastewater Introduction and Flow: The aquaculture wastewater 18 that has undergone anaerobic fermentation or other pretreatment is introduced into the aeration tank 14 , and the wastewater flows in the multi-stage tanks 24 in an overflow manner.

[0048] S2. Mixing and Spraying: After the wastewater is mixed with the bacterial bags and microalgae seed solution in the intermediate settling tank 16, it is evenly sprayed onto the vertical biological treatment membrane bed unit 1 through the spray pipe 4, distributing the bacteria and microalgae on the wind screen. The wastewater then flows back to the water tank 24, continuing the cycle.

[0049] S3. Biofilm Formation: After 1-5 days, a stable biofilm, 2-5mm thick, is formed by microorganisms trapped in the bacterial bags, microalgae seed solution, and air. The biofilm composition automatically adjusts to regional, temperature, and light conditions.

[0050] S4. Wastewater treatment and concentration: The wastewater is concentrated to 10%-30% of its original volume after natural evaporation with a hydraulic retention time of 3-15 days and a circulation rate of 7-15 m³ / h.

[0051] S5. Biofilm cleaning and utilization: Partially clean the vertical biotreatment membrane bed unit every 6-8 weeks, retaining 25%-50% of the original biofilm. The cleaned biofilm can be used to extract bioactive components or natural biological materials.

[0052] S6. Utilization of concentrated tail water: The treated concentrated tail water is used as a high-efficiency microalgae culture medium for subsequent large-scale microalgae cultivation. It can be connected to external circular pools, runway pools 10, bag-type photoreactors and other equipment for continuous cultivation.

[0053] Application example 1: The one-year application study of this equipment was carried out at the Guosheng Breeding and Farming Professional Cooperative Breeding Base. The indicators of the pig breeding wastewater tail water treated by black film anaerobic treatment in the base were as follows: Total C 2600mg / L, total N 1350mg / L, total P 340mg / L, total suspended solids TSS 2.28mg / L.

[0054] The indicators after being processed by the above device are: Total C 1200 mg / L, total N 1800 mg / L, total P 340 mg / L, total suspended solids TSS 0.58 mg / L.

[0055] In this example, the treated tailwater was concentrated to 1 / 10 of the original wastewater volume. Based on the total element content, the removal rates for total C, total N, total P, and total suspended solids were 95.38%, 86.72%, 90.00%, and 97.46%, respectively. When used as a high-efficiency microalgae culture medium, the solution was diluted appropriately at a ratio of 1:25-1:50 based on the nutritional requirements of different microalgae.

[0056] Application Example 2: Application of High-Efficiency Microalgae Culture Medium See also Figure 4-6 This culture medium can be used for large-scale cultivation of various microalgae species, including but not limited to the Chlorella sorokinensis described in this application example ( Chlorella sorokiniana ) and Nitzschia granatum ( Nitzschia palea ); and is applicable to a variety of different photobioreactors that can be used for microalgae cultivation, including but not limited to the tubular photobioreactor and open flat-plate photobioreactor described in this application example.

[0057] In this application example, Jiangxi Heyi Biotechnology Co., Ltd. C. sorokiniana Conduct application research on microalgae culture medium for experimental subjects.

[0058] The pipeline reactor described in this application example has a working volume of 100t and a total pipeline length of 8000m.

[0059] In this application example, C. sorokiniana The inoculum biomass was 5·10 6 -10 7 cfu / mL, the total culture time was 96 h, natural light was used during the culture process, and the biomass at the end of the culture was 6·10 7 -7.8•10 7 cfu / mL, and the dry matter mass of the algal liquid was 2.0 g / L.

[0060] In this application example, Jiangxi Heyi Biotechnology Co., Ltd. N. palea Conduct application research on microalgae culture medium for experimental subjects.

[0061] The open flat-plate reactor described in this application example has a working volume of 15 L and a total light receiving area of 1 m 2 .

[0062] In this application example, N. palea The inoculum biomass was 2·10 5 cfu / mL, the total culture time was 96 h, 18 W fluorescent tubes were used for supplementary lighting during the culture process, and the biomass at the end of the culture was 6·10 6 cfu / mL, and the dry matter mass of the algal liquid was 2.3 g / L.

[0063] At the same time, the present invention also discloses the application of an aquaculture wastewater treatment device based on a dynamic bacterial and algal biofilm, which is characterized in that the equipment is used for aquaculture wastewater treatment, the obtained concentrated tail water can be used for large-scale cultivation of microalgae, and the obtained biofilm can be used to extract biologically active substances or natural biological materials.

[0064] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A livestock and poultry breeding wastewater treatment system based on dynamic bacterial and algal biofilm technology, mainly consisting of a biological treatment membrane bed unit (1), a water tank (24), a biological filter material layer (25) and a water-air pumping system; wherein, The biological treatment membrane bed unit (1) is fixed on the top of the water tank (24), and multiple groups of suspended biofilm beds (2) are arranged inside the water tank (24); the water tank (24) is divided into three functional areas, namely, an aeration tank (14), an intermediate sedimentation tank (16), and a conversion tank (27) by a wall (20), and the aquaculture wastewater (18) to be treated is stored in the tank; the intermediate sedimentation tank (16) is equipped with biological treatment materials such as Widmannian Bacillus bacteria bags (17), Escherichia coli bags (19), and microalgae seed liquid; the biological filter material layer (25) is arranged on the top of the water tank (24), and a gauze cushion layer is laid on the bottom; the wind screen in the biological treatment membrane bed unit (1) is made of one or more of polyester, nylon or other chemical fiber materials, and is black or dark gray in color, and has a coil or thread array rough surface made by a wire drawing process to assist in the formation of biofilm, the wind screen hanging density is 7-10 sheets / m, and the total working area of a single biofilm bed (2) is 12-20m 2 .

2. The livestock and poultry breeding wastewater treatment system based on dynamic bacteria and algae biofilm according to claim 1 is characterized in that: The water vapor pumping system includes a spray pipe (4) and a spray pump (5), one end of the spray pipe (4) is connected to the lower end of the water tank (24), and the spray pipe (4) is extended to the end of the biological treatment membrane bed unit (1) and is spaced apart with a spray device (3). A comprehensive treatment unit (6) is provided on one side of the water tank (24), and a negative pressure fractionator (8) is installed inside the comprehensive treatment unit (6). One side of the negative pressure fractionator (8) is connected to a moisture absorption pipe (7) communicating with the conversion tank (27), and the top of the negative pressure fractionator (8) is connected to a pipe extending to the comprehensive treatment unit (6). An external discharge pipe (26) is arranged outside the integrated treatment unit (6); an aeration pump (13) is installed inside the integrated treatment unit (6); an aeration pipe (22) connected to the aeration pump (13) is arranged inside the aeration tank (14); and an aeration head (21) is arranged at intervals at the top end of the aeration pipe (22); the aeration tank (14) is connected to the aquaculture wastewater tank through a water supply pipe (11) and a water supply pump (12); one side of the water tank (24) is connected to the runway tank (10) through a liquid delivery pipe (15), and a photobioreactor (9) is installed at one end of the runway tank (10).

3. The livestock and poultry breeding wastewater treatment system based on dynamic bacteria and algae biofilm according to claim 1 is characterized in that: The distribution density of the spraying device (3) is 7-10 pieces / m, and the flow rate of a single spraying device (3) is set to 150-250 L / h.

4. The livestock and poultry breeding wastewater treatment system based on dynamic bacteria and algae biofilm according to claim 1 is characterized in that: The biological filter material layer (25) is composed of rice husks or other corrosion-resistant plant-based biological materials, with a thickness of 150-350 mm, and the mesh size of the underlying gauze cushion layer is 50-100 meshes.

5. The livestock and poultry breeding wastewater treatment system based on dynamic bacteria and algae biofilm according to claim 1 is characterized in that: The distribution density of the aeration heads (21) is 2 / m, and the gas flow rate of a single aeration head (21) is 2000-5000 L / h.

6. The livestock and poultry breeding wastewater treatment system based on dynamic bacteria-algae biofilm according to claim 1 is characterized in that: The flow rate of the water supply pipe (11) is set to 10-15m 3 / h.

7. The livestock and poultry breeding wastewater treatment system based on dynamic bacteria-algae biofilm according to claim 1 is characterized in that: The application specifications of the Widmannian Bacillus package (17) and the Marmoset Escherichia package (19) are 10 8 -10 10 cfu / g.

8. The livestock and poultry breeding wastewater treatment system based on dynamic bacteria-algae biofilm according to claim 1 is characterized in that: The microalgae seed liquid includes but is not limited to one or more of Chlorella, Chlamydomonas, diatoms, and Anabaena.

9. A method for treating livestock and poultry breeding wastewater based on dynamic bacteria-algae biofilm according to any one of claims 1 to 8, characterized in that: The aquaculture wastewater treatment method comprises the following steps: S1. Wastewater introduction and flow: The aquaculture wastewater (18) that has undergone anaerobic fermentation or other pretreatment is introduced into the aeration tank (14), and the wastewater flows in the multi-stage tank of the water tank (24) in an overflow manner; S2. Mixing and spraying: After the wastewater is mixed with the bacterial bag and microalgae seed solution in the intermediate sedimentation tank (16), it is evenly sprayed onto the vertical biological treatment membrane bed unit (1) through the spray pipe (4), so that the bacteria and microalgae are distributed on the wind screen; The wastewater flows down and returns to the water tank (24), and the cycle continues; S3. Biofilm Formation: Microorganisms captured from the bacterial bags, microalgae seed solution, and air form a stable biofilm over 1-5 days, reaching a thickness of 2-5 mm, and entering a stable treatment state. The composition of the biofilm automatically adjusts to regional, temperature, light and other conditions; S4. Wastewater treatment and concentration: The wastewater is concentrated to 10%-30% of its original volume through natural evaporation with a hydraulic retention time of 3-15 days and a circulation rate of 7-15 m³ / h. S5. Biofilm Cleaning and Utilization: Partially clean the vertical biotreatment membrane bed unit every 6-8 weeks, retaining 25%-50% of the original biofilm. The cleaned biofilm can be used to extract bioactive components or natural biomaterials. S6. Utilization of concentrated tail water: The treated concentrated tail water is used as a high-efficiency microalgae culture medium for subsequent large-scale microalgae cultivation. It can be connected to circular pools, runway pools (10), bag-type photoreactors and other equipment for continuous cultivation.

10. The use of a dynamic bacteria-algae biofilm-based aquaculture wastewater treatment device according to claims 1-8, characterized in that: The equipment is used for aquaculture wastewater treatment, the concentrated tail water obtained can be used for large-scale cultivation of microalgae, and the biofilm obtained can be used for extracting bioactive substances or natural biological materials.

Images