On-site large-scale culture device suitable for MICP (Microbial Inductively Coupled Plasma) microbial fermentation

Abstract

The invention discloses an on-site large-scale culture device suitable for MICP microbial fermentation, and relates to the technical field of microbial culture.The on-site large-scale culture device comprises a fermentation barrel, oxygen pumps are evenly arranged at the bottom of the fermentation barrel, double-screw pumps are symmetrically arranged in the middle of the fermentation barrel, intelligent control boxes are installed on the double-screw pumps, and an exhaust pipe is arranged on the upper surface of the fermentation barrel; according to the scheme, bacterial liquid is dynamically pumped and discharged, so that the double-screw pump can pump and discharge the bacterial liquid at different positions of the bottom of the fermentation barrel, and the problems that a traditional stirring device cannot avoid precipitation, the activity of the bacterial liquid at the bottom is not uniformly distributed, and the fermentation efficiency is low can be solved. The bacteria liquid at the bottom of the fermentation barrel is uniformly and dynamically pumped to the upper part of the fermentation barrel through the double-screw pump, and the bacteria liquid at the bottom of the culture device is pumped to the upper part through the double-screw pump, so that an up-and-down circulating mixing mode is formed, and the mixing mode can break the concentration gradient in the bacteria liquid, so that nutrient substances, oxygen and the like are uniformly distributed; the growth rate and the metabolic activity of the thalli are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial culture, in particular to an on-site large-scale culture device suitable for MICP microbial fermentation. Background Art

[0002] MICP (Microbially Induced Calcium Carbonate Precipitation) technology is widely used in fields such as soil reinforcement and concrete remediation. Existing technologies primarily rely on laboratory-based microbial culture equipment. These technologies are characterized by: fixed laboratory equipment, such as fermentation tanks and shakers, which require a stable power supply and temperature control; small-scale culture models, which are large and complex, making them difficult to apply directly to construction sites; manual operation, which requires frequent monitoring of pH, temperature, and bacterial activity, and is unsuitable for continuous on-site operations; and outdoor microbial culture, which is often performed manually in traditional fermentation tanks with overhead agitators or air pumps for aeration. This results in easy sedimentation of the bacterial solution at the bottom, low mixing efficiency, and limited control over bacterial activity.

[0003] In summary, existing microbial cultivation equipment has the following disadvantages:

[0004] (1) Inability to apply on-site on a large scale: Laboratory equipment is large in size, consumes high energy, and relies on fixed infrastructure (such as constant temperature air conditioning), making it difficult to deploy in the wild or in an environment without power supply.

[0005] (2) Uneven mixing of bacterial liquid: Traditional stirring devices cannot avoid sedimentation, resulting in uneven distribution of bacterial liquid activity at the bottom and unstable quality during large-scale culture (structural defect: the stirrer is located at the top of the container, making it difficult to stir the sediment at the bottom).

[0006] (3) Inaccurate temperature control: External heating methods respond slowly and are difficult to control temperature evenly (principle defect: relying on an external heat source and not in direct contact with the bacterial solution).

[0007] (4) Lack of monitoring of bacterial liquid activity: There is a lack of real-time monitoring methods such as conductivity. Manual detection of parameters such as pH and activity cannot provide real-time feedback, which affects the culture quality.

[0008] Therefore, an on-site large-scale culture device suitable for MICP microbial fermentation is proposed to solve the above problems. Summary of the Invention

[0009] In view of this, the technical problem to be solved by the present invention is to propose an on-site large-scale culture device suitable for MICP microbial fermentation to solve the problems arising from the background technology.

[0010] To achieve the above objectives, the present invention provides the following technical solutions: an on-site large-scale culture device suitable for MICP microbial fermentation, comprising a fermentation barrel, oxygen pumps evenly arranged at the bottom of the fermentation barrel, a twin-screw pump symmetrically arranged in the middle of the fermentation barrel, an intelligent control box installed on the twin-screw pump, an exhaust pipe opened on the upper surface of the fermentation barrel, and a two-way valve installed on the outer surface of the middle of the fermentation barrel. The on-site large-scale culture device suitable for MICP microbial fermentation further includes a multi-point monitoring aeration mechanism and a reflux regulation mechanism;

[0011] The multi-point monitoring aeration mechanism is arranged at the bottom of the fermentation barrel, and is used to precipitate, stir and uniformly aerate the culture solution at the bottom of the fermentation barrel;

[0012] The reflux regulating mechanism is arranged above the multi-point monitoring aeration mechanism, and the reflux regulating mechanism is used for dynamic detection of the culture solution and prevention of precipitation.

[0013] Preferably, the multi-point monitoring aeration mechanism includes a sedimentation scraper, the middle part of the sedimentation scraper is rotatably connected to the bottom of the fermentation barrel, the oxygen pump is evenly installed on the sedimentation scraper, the middle part of the sedimentation scraper is fixedly connected to a transmission shaft, the outer surface of the transmission shaft is fixedly connected to a first transmission tooth, and the tooth surface of the first transmission tooth is meshed with a second transmission tooth.

[0014] Preferably, an inner gear disc is provided at the bottom of the second transmission tooth, an engaging groove is opened in the middle of the inner gear disc, the second transmission tooth is evenly rotated and connected to the middle of the inner gear disc, the tooth surface of the second transmission tooth away from the first transmission tooth is engaged in the engaging groove opened in the inner gear disc, and the outer surface of the inner gear disc is evenly installed with arc-surface push-flow plates.

[0015] Preferably, the arc-surface flow-pushing plate is evenly provided with guide holes, the upper end of the transmission shaft is fixedly connected to a connecting plate, the inner wall of the fermentation barrel is rotatably connected to a rotating ring, both ends of the connecting plate are fixedly connected to the rotating ring, and the twin-screw pump is symmetrically mounted on the connecting plate.

[0016] Preferably, the reflux regulating mechanism includes a filter cover, which is installed at the bottom of the twin-screw pump. A servo drive motor is installed on the twin-screw pump, and a drive tooth is fixedly connected to the drive shaft of the servo drive motor. The inner surface of the filter cover is rotatably connected to an inner gear ring, and a sliding tooth groove is provided on the outer surface of the inner gear ring, and the tooth surface of the drive tooth is engaged in the sliding tooth groove of the inner gear ring.

[0017] Preferably, the inner tooth surface of the inner gear ring is engaged with a third transmission tooth, and the third transmission tooth is circumferentially connected to the filter cover, a guide groove is opened on the circumference of the filter cover, and an L-shaped multi-function plate is arranged on the circumference of the filter cover, and sliding teeth are evenly arranged on the L-shaped multi-function plate, and the tooth surface of the L-shaped multi-function plate sliding tooth is engaged with the third transmission tooth.

[0018] Preferably, the L-shaped multifunctional plate is fixedly connected to a fixed block on the side close to the sliding tooth, and the fixed block is slidably connected in the guide groove. The L-shaped multifunctional plate is fixedly connected to a scraper on the end away from the sliding tooth, and the scraper slides on the upper surface of the filter hole at the bottom of the filter cover.

[0019] Preferably, a resistance wire and a temperature sensor are provided inside the twin-screw pump body to achieve rapid preheating of the bacterial solution.

[0020] Compared with the prior art, the present invention provides an on-site large-scale culture device suitable for MICP microbial fermentation, which has the following beneficial effects:

[0021] 1. On-site large-scale cultivation: The modular design supports parallel connection of multiple devices. The cultivation capacity of a single fermentation barrel can reach 500L, which can be expanded to thousands of liters after parallel connection to meet the needs of the project site.

[0022] 2. This solution abandons the traditional top-mounted stirring device, which easily causes the bacterial liquid to settle at the bottom. The twin-screw pump is used to dynamically rotate in the bacterial barrel to prevent the bacterial liquid from settling.

[0023] 3. This solution adopts dynamic aeration at the bottom of the fermentation barrel. Compared with traditional fixed aeration, it makes the bacterial liquid more uniform in aerobic respiration and reduces the death of bacterial colonies due to lack of oxygen.

[0024] 4. Dynamically collect pH-related data of bacterial liquid to reduce the error of single-area collection.

[0025] 5. The resistance wire on the inner wall of the cylinder and the cavity on the inner wall of the twin-screw pump are heated by the resistance wire, and the bacterial liquid is extracted by hot water heat exchange and the temperature is controlled at the same time.

[0026] 6. Intelligence and environmental protection:

[0027] (1) Real-time monitoring data is transmitted wirelessly to mobile terminals to achieve remote monitoring;

[0028] (2) The silent fan is combined with an activated carbon filter layer to prevent the spread of odor.

[0029] (3) The conductivity needle is linked to the pH sensor to reflect the metabolic status of microorganisms in real time.

[0030] (4) Automated control based on multi-parameter feedback reduces manual intervention and improves cultivation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0032] Figure 2 It is a half-cut schematic diagram of the three-dimensional structure of the present invention;

[0033] Figure 3 For the present invention Figure 2 Enlarged view of point A in the middle;

[0034] Figure 4 This is a schematic diagram of the structural connection relationship of the reflux regulating mechanism of the present invention;

[0035] Figure 5 For the present invention Figure 4 Enlarged view of point B in the middle;

[0036] Figure 6 This is an auxiliary schematic diagram of the structural connection relationship of the reflux regulating mechanism of the present invention;

[0037] Figure 7 This is a diagram showing the structural connection relationship of the L-shaped multifunctional board of the present invention;

[0038] Figure 8 This is a flow chart of automatic temperature control of the present invention.

[0039] In the picture:

[0040] 1. Fermentation barrel; 11. Oxygen pump; 12. Twin-screw pump; 13. Intelligent control box; 14. Exhaust pipe; 15. Two-way valve;

[0041] 2. Multi-point monitoring aeration mechanism; 21. Sedimentation scraper; 22. Drive shaft; 23. First drive gear; 24. Second drive gear; 25. Internal gear plate; 26. Surface push plate; 27. Connecting plate; 28. Rotating ring;

[0042] 3. Backflow adjustment mechanism; 31. Filter cover; 32. Drive gear; 33. Internal gear ring; 34. Third transmission gear; 35. L-shaped multifunctional plate; 36. Scraper; 37. Guide groove; 38. Fixed block. DETAILED DESCRIPTION

[0043] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0044] The present invention will be described in further detail below with reference to the accompanying drawings and examples.

[0045] For example, please refer to Figures 1 to 8 As shown:

[0046] To solve the problems mentioned in the technical solution, the embodiment of the present application provides an on-site large-scale culture device suitable for MICP microbial fermentation, including a fermentation barrel 1, an oxygen pump 11 is evenly arranged at the bottom of the fermentation barrel 1, a twin-screw pump 12 is symmetrically arranged in the middle of the fermentation barrel 1, an intelligent control box 13 is installed on the twin-screw pump 12, an exhaust pipe 14 is opened on the upper surface of the fermentation barrel 1, a two-way valve 15 is installed on the outer surface of the middle of the fermentation barrel 1, and further includes a multi-point monitoring aeration mechanism 2 and a reflux regulation mechanism 3;

[0047] The multi-point monitoring aeration mechanism 2 is arranged at the bottom of the fermentation tank 1, and the multi-point monitoring aeration mechanism 2 is used to precipitate, stir and uniformly aerate the culture solution at the bottom of the fermentation tank 1;

[0048] The reflux regulating mechanism 3 is arranged above the multi-point monitoring aeration mechanism 2 and is used for dynamic detection of the culture solution and prevention of precipitation;

[0049] Among them, the inner wall of the fermentation barrel 1 is embedded with a resistance wire, and the intelligent control box 13 is equipped with a pH sensor, a temperature sensor, a control logic unit adaptive adjustment algorithm, and a conductivity sensor. The conductivity sensor is used to quantitatively evaluate the activity of microorganisms. The twin-screw pump 12 is provided with a resistance wire and a temperature sensor inside the pump body to achieve rapid preheating of the bacterial solution.

[0050] like Figure 4 As shown, this solution provides a heat exchange chamber on the inner wall of 12, and a temperature sensor is installed in the heat exchange chamber, and a resistance wire is installed in the heat exchange chamber. The temperature sensor can control the heating of the resistance wire, and the hot water can be added to the heat exchange chamber through the sealing cover that can be threaded and rotated on the side of the drive motor above 12. The heating of the hot water by the resistance wire can achieve heat exchange with the bacterial liquid through the inner wall of the twin-screw pump 12 after the heated water is heated, so that the temperature of the bacterial liquid can be controlled more accurately. The temperature of the culture liquid can be controlled at all times by the twin-screw pump 12 and the temperature control device inside the fermentation barrel 1, and the bacterial liquid can be maintained at a constant temperature of ±1°C in an environment of -10°C to 40°C, adapting to extreme temperatures in the field.

[0051] The specific temperature control process is as follows Figure 8 shown.

[0052] In this solution, pulleys are evenly installed on the lower surface of the fermentation barrel 1, and the fermentation barrel 1 can be quickly moved by the pulleys. At the same time, two-way valves 15 are installed on both sides of the fermentation barrel 1. When facing the demand for mixed culture of larger bacterial liquids, it is only necessary to add a fermentation barrel 1 and quickly connect the two-way valve 15. The modular design supports parallel connection of multiple devices in the fermentation barrel 1. The culture capacity of a single set of devices in the fermentation barrel 1 can reach 500L, which can be expanded to thousands of liters after parallel connection. It is detachable and combinable, can adapt to complex on-site working conditions, and meet the needs of the engineering site. At the same time, the temperature of the culture liquid can be controlled at all times by the twin-screw pump 12 and the temperature control device inside the fermentation barrel 1. The bacterial liquid can be maintained at a constant temperature of ±1°C in an environment of -10°C to 40°C, adapting to extreme temperatures in the field.

[0053] Specifically, such as Figure 3 As shown, the middle part of the sedimentation scraper 21 is rotatably connected to the bottom of the fermentation barrel 1, and the oxygen pump 11 is evenly installed on the sedimentation scraper 21. The middle part of the sedimentation scraper 21 is fixedly connected to the transmission shaft 22, and the outer surface of the transmission shaft 22 is fixedly connected to the first transmission tooth 23, and the tooth surface of the first transmission tooth 23 is meshed with the second transmission tooth 24; the bottom of the second transmission tooth 24 is provided with an inner gear disc 25, and the middle part of the inner gear disc 25 is provided with an engaging groove. The second transmission tooth 24 is evenly rotatably connected to the middle part of the inner gear disc 25, and the tooth surface of the second transmission tooth 24 away from the first transmission tooth 23 is meshed in the engaging groove provided in the inner gear disc 25, and the outer surface of the inner gear disc 25 is evenly installed with an arc-surface pushing plate 26; the arc-surface pushing plate 26 is evenly provided with guide holes.

[0054] A driving motor is installed at the bottom of the fermentation barrel 1, which drives the middle part of the sedimentation scraper 21 to rotate through the driving motor. The rotation of the sedimentation scraper 21 drives the first transmission tooth 23 to drive the second transmission tooth 24 to rotate in the opposite direction. The rotation of the second transmission tooth 24 drives the inner gear disc 25 to rotate synchronously again. In this scheme, the scraper at the bottom of the sedimentation scraper 21 can be used to scrape the sediment at the bottom of the fermentation barrel 1 to prevent uniform sediment from adhering to the bottom of the fermentation barrel 1. The bottom of the fermentation barrel 1 can be stirred by the rotation and scraping of the sedimentation scraper 21 and the inner gear disc 25 rotating in the opposite direction relative to the sedimentation scraper 21. The arc-surface pushing plate 26 uniformly welded on the inner gear disc 25 can accelerate the flow of the bacteria liquid at the bottom of the fermentation barrel 1 through the guide holes on the arc-surface pushing plate 26, thereby preventing the sedimentation of the bacteria liquid again.

[0055] Furthermore, the oxygen pump 11 is evenly installed on the sedimentation scraper 21, and the oxygen pump 11 is used to aerate the bottom of the fermentation barrel 1. Compared with the traditional technology of fixing the aeration at the bottom of the fermentation barrel 1, local aeration of the culture liquid occurs. Through the arrangement of this scheme, the oxygen pump 11 is used to rotate synchronously with the sedimentation scraper 21. When the sedimentation scraper 21 cleans and stirs the bottom of the fermentation barrel 1, the oxygen pump 11 can simultaneously aerate the bottom of the fermentation barrel 1. The rotation of the oxygen pump 11 can achieve uniform aeration coverage of the bottom of the fermentation barrel 1, thereby reducing the occurrence of local aeration, allowing the bacterial liquid to undergo a more uniform aerobic reaction, and increasing the uniformity of the uniform culture.

[0056] Furthermore, the upper end of the transmission shaft 22 is fixedly connected to a connecting plate 27, the inner wall of the fermentation barrel 1 is rotatably connected to a swivel 28, both ends of the connecting plate 27 are fixedly connected to the swivel 28, and the twin-screw pump 12 is symmetrically mounted on the connecting plate 27;

[0057] This solution adopts the method of synchronously driving the rotation of the inner gear disc 25 and the connecting plate 27 through the transmission shaft 22, so that the connecting plate 27 rotates in the opposite direction relative to the inner gear disc 25, and drives the twin-screw pump 12 to rotate evenly in the fermentation barrel 1 through the connecting plate 27. Compared with the fixed twin-screw pump 12 installation, this solution adopts dynamic pumping of the bacterial liquid, so that the twin-screw pump 12 can pump and discharge the bacterial liquid at different positions at the bottom of the fermentation barrel 1, thereby avoiding the unavoidable precipitation of the traditional stirring device, resulting in uneven distribution of the activity of the bacterial liquid at the bottom. The bacterial liquid at the bottom of the fermentation barrel 1 is evenly and dynamically pumped to the top of the fermentation barrel 1 by the twin-screw pump 12, and the twin-screw pump 12 pumps the bacterial liquid at the bottom of the incubator to the upper part, forming an up-and-down circulation mixing mode. This mixing mode can break the concentration gradient in the bacterial liquid, evenly distribute nutrients, oxygen, etc., and improve the growth rate and metabolic activity of the bacteria.

[0058] Specifically, such as Figure 6 As shown, a servo drive motor is installed on the twin-screw pump 12, and a drive gear 32 is fixedly connected to the drive shaft of the servo drive motor. The inner surface of the filter cover 31 is rotatably connected to an inner gear ring 33. The outer surface of the inner gear ring 33 is provided with a sliding tooth groove, and the tooth surface of the drive gear 32 is engaged with the sliding tooth groove of the inner gear ring 33;

[0059] The servo motor rotates to drive the driving gear 32 to drive the inner gear ring 33 to rotate and adjust in the inner wall of the filter cover 31.

[0060] The inner tooth surface of the inner gear ring 33 is meshed with a third transmission tooth 34, and the third transmission tooth 34 is rotatably connected to the filter cover 31. A guide groove 37 is provided on the circumference of the filter cover 31. An L-shaped multifunctional plate 35 is provided on the circumference of the filter cover 31. Sliding teeth are evenly provided on the L-shaped multifunctional plate 35. The sliding tooth surface of the L-shaped multifunctional plate 35 meshes with the third transmission tooth 34; a fixed block 38 is fixedly connected to the side of the L-shaped multifunctional plate 35 close to the sliding teeth, and the fixed block 38 is slidably connected to the guide groove 37. A scraper 36 is fixedly connected to the end of the L-shaped multifunctional plate 35 away from the sliding teeth, and the scraper 36 slides on the upper surface of the filter hole at the bottom of the filter cover 31;

[0061] Among them, such as Figure 6 As shown, the L-shaped multifunctional plate 35 is arranged in an L-shape. The sliding movement of the L-shaped multifunctional plate 35 controls the size of the liquid inlet of the twin-screw pump 12. Simultaneously, the sliding of the cleaning plate 36 across the surface of the filter screen 31 prevents clogging of the filter screen 31 caused by bacterial liquid accumulation. By controlling the size of the liquid inlet of the twin-screw pump, this solution allows precise adjustment of the bacterial liquid delivery rate to meet the needs of different culture stages. In the early stages of culture, the liquid inlet size can be reduced to avoid excessive shear forces on the bacteria. In the later stages of culture, the liquid inlet size can be increased to accelerate mixing. Mixing by the twin-screw pump 12 prevents bacterial sedimentation at the bottom of the incubator, reducing bacterial death and degradation.

[0062] The specific implementation steps in the above embodiment are as follows:

[0063] Step 1: assemble and start the fermentation barrel 1 on site; transport the culture unit of the fermentation barrel 1 to the project site, connect the pipeline, power supply and control module through the two-way valve 15 quick interface; inject preheated water into the twin-screw pump 12, set the initial temperature according to the environment, and start the internal circulation.

[0064] The specific steps are as follows:

[0065] First, start the drive motor installed at the bottom of the fermentation tank 1, and then start the drive motor to drive the Figure 3The sedimentation scraper 21 shown starts to rotate synchronously. The rotation of the sedimentation scraper 21 can clean the bottom of the fermentation barrel 1 to prevent the bacterial liquid from depositing and adhering to the bottom of the fermentation barrel 1. At the same time, the transmission shaft 22 drives the inner gear disc 25 through the engagement of the second transmission gear 24 to drive the cambered surface flow-pushing plate 26 to rotate in the opposite direction relative to the sedimentation scraper 21. The rotation of the cambered surface flow-pushing plate 26 can accelerate the stirring of the bacterial liquid at the bottom of the fermentation barrel 1. At the same time, the flow guide holes evenly opened on the cambered surface flow-pushing plate 26 can accelerate the flow of the bacterial liquid at the bottom of the fermentation barrel 1. At the same time, the transmission of the transmission shaft 22 can synchronously drive the connecting plate 27 to drive the twin-screw pump 12 to rotate in the fermentation barrel 1, and drive the connecting plate 27 to drive the The twin-screw pump 12 rotates evenly in the fermentation barrel 1. Compared with the fixed twin-screw pump 12 installation, this solution adopts dynamic pumping of the bacterial liquid, so that the twin-screw pump 12 can pump and discharge the bacterial liquid at different positions at the bottom of the fermentation barrel 1, thereby avoiding the precipitation that cannot be avoided by the traditional stirring device, resulting in uneven distribution of the activity of the bacterial liquid at the bottom. The twin-screw pump 12 uniformly and dynamically pumps the bacterial liquid at the bottom of the fermentation barrel 1 to the top of the fermentation barrel 1, and the twin-screw pump 12 pumps the bacterial liquid at the bottom of the incubator to the upper part, forming an up-and-down circulation mixing mode. This mixing mode can break the concentration gradient in the bacterial liquid, make nutrients, oxygen, etc. evenly distributed, and improve the growth rate and metabolic activity of the bacteria.

[0066] Step 2: Bacterial liquid loading and expansion.

[0067] Inject bacterial liquid and culture medium into the first set of fermentation barrels 1, gradually connect the new units of fermentation barrel 1 through the two-way valve 15, the system automatically balances the flow of each unit, pours culture medium into the new fermentation barrel 1, opens the two-way valve 15 between the first set of fermentation barrels 1 and the new fermentation barrel 1, and transfers the cultured bacteria in the first set of fermentation barrels 1 to the new fermentation barrel 1. The cycle continues until the fermentation barrels 1 are full of bacteria. At this time, bacteria with different activities can be obtained, and bacteria with the same activity can be obtained at different time periods.

[0068] Step 3: Intelligent control example.

[0069] The resistance wire embedded in the inner wall of the fermentation barrel 1 is started to heat the ambient temperature. When the ambient temperature reaches the specified temperature, the control unit increases the power of the resistance wire and simultaneously speeds up the flow rate of the twin-screw pump 12 to balance the heat.

[0070] The specific operation process is as follows by controlling the size of the liquid inlet at the bottom of the twin-screw pump 12:

[0071] First, the control system in the intelligent control box 13 is used to control the servo motor installed on the twin-screw pump 12 to start. At this time, the rotation of the servo motor can drive the driving gear 32 to drive the inner gear ring 33 to rotate. When the inner gear ring 33 rotates, the inner tooth surface of the inner gear ring 33 and the L-shaped multi-function plate 35 engage with each other, and the rotation of the inner gear ring 33 can drive the third transmission gear 34 to drive the L-shaped multi-function plate 35 to slide on the filter cover 31. By controlling the sliding between the L-shaped multi-function plates 35, the size of the liquid inlet between the L-shaped multi-function plate 35 and the twin-screw pump 12 can be controlled. By controlling the size of the liquid inlet of the twin-screw pump, the delivery amount of the bacterial liquid can be accurately adjusted to meet the needs of different culture stages; in the early stage of culture, the size of the liquid inlet can be reduced to avoid excessive shear force on the bacteria; in the later stage of culture, the size of the liquid inlet can be increased to speed up the mixing speed. Mixing by the twin-screw pump 12 can prevent the bacteria from depositing at the bottom of the culture vessel and reduce the death and degradation of the bacteria.

[0072] Furthermore, buffer solution is automatically injected when pH exceeds the limit; the oxygen meter is started at the bottom of the fermentation barrel at regular intervals to enhance bacterial activity. When the conductivity needle detects a decrease in activity, the oxygen pump 11 is automatically triggered for pressurized aeration. Since the oxygen pump 11 is installed on the sedimentation scraper 21, the rotation of the sedimentation scraper 21 can synchronously drive the oxygen pump 11 to start rotating. In this solution, the oxygen pump 11 is rotated synchronously with the sedimentation scraper 21. When the sedimentation scraper 21 cleans and stirs the bottom of the fermentation barrel 1, the bottom of the fermentation barrel 1 can be aerated synchronously through the oxygen pump 11. The rotation of the oxygen pump 11 can achieve uniform aeration coverage of the bottom of the fermentation barrel 1, thereby reducing the occurrence of local aeration, allowing the bacterial liquid to undergo aerobic reaction more evenly, and increasing the uniformity of uniform culture.

[0073] like Figure 8 As shown, when a sensor installed in the heat exchange chamber (12) detects that the bacterial solution has not reached the appropriate temperature, the system automatically starts to read the real-time temperature of the current temperature sensor. At this time, the control circuit in the system compares the received electrical signal with a preset temperature value, which is set according to the needs of bacterial culture and is usually a constant value. When the control circuit detects that the actual temperature of the bacterial solution is lower than the preset temperature, it sends a control signal, which activates the resistance wire to heat the water in the heat exchange chamber. The heat generated by the resistance wire is transferred to the water in the heat exchange chamber. Through the heat exchange between the water and the inner wall (12), the inner wall (12) controls the temperature of the bacterial solution, thereby gradually increasing the temperature of the bacterial solution.

[0074] During the heating process, the temperature sensor continuously monitors the temperature of the bacterial solution. When the temperature approaches the preset value, the temperature sensor feeds this information back to the control circuit. The control circuit then adjusts the heating power of the resistance wire based on this feedback signal to ensure that the temperature of the bacterial solution remains stable near the preset value. Through the temperature sensor's continuous monitoring and the control circuit's adjustment, the temperature of the bacterial solution will eventually stabilize near the preset value, creating a stable temperature control environment.

[0075] Step 4: Exhaust treatment.

[0076] A silent fan is installed in the exhaust pipe 14. The silent fan adjusts its speed according to the gas sensor data, and the exhaust port is connected to an extension pipe to an unmanned area 30 meters away.

[0077] Please refer to the above working process Figures 1 to 8 .

[0078] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply the existence of any such actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article, or device comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or elements inherent to such process, method, article, or device. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other identical elements in the process, method, article, or device comprising the element.

[0079] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. An on-site large-scale culture device suitable for MICP microbial fermentation, characterized in that: The invention comprises a fermentation barrel (1), wherein an oxygen pump (11) is evenly arranged at the bottom of the fermentation barrel (1), a twin-screw pump (12) is symmetrically arranged in the middle of the fermentation barrel (1), an intelligent control box (13) is installed on the twin-screw pump (12), an exhaust pipe (14) is opened on the upper surface of the fermentation barrel (1), and a two-way valve (15) is installed on the outer surface of the middle part of the fermentation barrel (1). The on-site large-scale culture device suitable for MICP microbial fermentation also includes a multi-point monitoring aeration mechanism (2) and a reflux regulation mechanism (3); The multi-point monitoring aeration mechanism (2) is arranged at the bottom of the fermentation barrel (1), and the multi-point monitoring aeration mechanism (2) is used to stir and uniformly aerate the culture solution at the bottom of the fermentation barrel (1); The reflux regulating mechanism (3) is arranged above the multi-point monitoring aeration mechanism (2), and the reflux regulating mechanism (3) is used for dynamic detection of the culture solution and for preventing precipitation.

2. The on-site large-scale culture device suitable for MICP microbial fermentation according to claim 1, characterized in that: The multi-point monitoring aeration mechanism (2) includes a sedimentation scraper (21), the middle portion of the sedimentation scraper (21) is rotatably connected to the bottom of the fermentation barrel (1), the oxygen pump (11) is evenly installed on the sedimentation scraper (21), the middle portion of the sedimentation scraper (21) is fixedly connected to a transmission shaft (22), the outer surface of the transmission shaft (22) is fixedly connected to a first transmission tooth (23), and the tooth surface of the first transmission tooth (23) is meshed with a second transmission tooth (24).

3. The on-site large-scale culture device suitable for MICP microbial fermentation according to claim 2, characterized in that: An inner tooth disc (25) is provided at the bottom of the second transmission tooth (24), and an engagement groove is provided in the middle of the inner tooth disc (25). The second transmission tooth (24) is evenly rotated and connected to the middle of the inner tooth disc (25). The tooth surface of the second transmission tooth (24) away from the first transmission tooth (23) is engaged in the engagement groove provided in the inner tooth disc (25), and an arc-surface pusher plate (26) is evenly installed on the outer surface of the inner tooth disc (25).

4. The on-site large-scale culture device suitable for MICP microbial fermentation according to claim 3, characterized in that: The arc-surface flow-pushing plate (26) is evenly provided with flow guide holes, the upper end of the transmission shaft (22) is fixedly connected to a connecting plate (27), the inner wall of the fermentation barrel (1) is rotatably connected to a rotating ring (28), the two ends of the connecting plate (27) are fixedly connected to the rotating ring (28), and the twin-screw pump (12) is symmetrically mounted on the connecting plate (27).

5. The on-site large-scale culture device suitable for MICP microbial fermentation according to claim 1, characterized in that: The reflux regulating mechanism (3) comprises a filter cover (31), the filter cover (31) being mounted on the bottom of a twin-screw pump (12), a servo drive motor being mounted on the twin-screw pump (12), and a drive tooth (32) being fixedly connected to a drive shaft of the servo drive motor, an inner gear ring (33) being rotatably connected to the inner surface of the filter cover (31), a sliding tooth groove being formed on the outer surface of the inner gear ring (33), and a tooth surface of the drive tooth (32) being meshed in the sliding tooth groove of the inner gear ring (33).

6. The on-site large-scale culture device suitable for MICP microbial fermentation according to claim 5, characterized in that: The inner tooth surface of the inner gear ring (33) is meshed with a third transmission tooth (34), and the third transmission tooth (34) is circumferentially connected to the filter cover (31). A guide groove (37) is provided on the circumference of the filter cover (31). An L-shaped multifunctional plate (35) is provided on the circumference of the filter cover (31). Sliding teeth are evenly provided on the L-shaped multifunctional plate (35), and the sliding tooth surface of the L-shaped multifunctional plate (35) is meshed with the third transmission tooth (34).

7. The on-site large-scale culture device suitable for MICP microbial fermentation according to claim 6, characterized in that: The L-shaped multifunctional plate (35) is fixedly connected to a fixed block (38) on one side close to the sliding teeth, and the fixed block (38) is slidably connected to the guide groove (37). The L-shaped multifunctional plate (35) is fixedly connected to an end away from the sliding teeth with a cleaning plate (36), and the cleaning plate (36) slides on the upper surface of the filter hole at the bottom of the filter cover (31).

8. The on-site large-scale culture device suitable for MICP microbial fermentation according to claim 1, characterized in that: A resistance wire and a temperature sensor are provided inside the pump body of the twin-screw pump (12) for realizing rapid preheating of the bacterial liquid.

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