Mesenchymal stem cell extraction equipment

Abstract

The mesenchymal stem cell extraction equipment comprises an extraction body, and a motor and a clutch transmission mechanism are arranged in the center of the bottom of the extraction body; a first turntable with a central hole is rotationally arranged in the extraction body; a central shaft penetrating into the central hole is arranged in the center of the top of the first turntable; a vibration ring is movably arranged in the first turntable in a lifting manner, and is fixedly provided with a container unit; the central shaft is rotationally sleeved with a second rotating disc, a first guide groove and a second guide groove are formed in the peripheral wall of the second rotating disc, and the second guide groove penetrates through the second rotating disc; a locking groove is formed in the peripheral wall of the second rotating disc; a second friction block is arranged in the second rotating disc, and an extrusion boss is arranged on the second friction block; a first bayonet lock extends from the inner wall of the vibrating ring; a second bayonet lock is elastically arranged at the end part of the first bayonet lock; therefore, the treatment of tissue separation and filtration, chemical enzymolysis and centrifugal layering is completed in single extraction equipment, the cell extraction efficiency and quality are remarkably improved, and the operation difficulty and the extraction cost are reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of mesenchymal stem cell extraction, and in particular to a mesenchymal stem cell extraction device. Background Art

[0002] Mesenchymal stem cells (MSCs) have attracted widespread attention in the fields of regenerative medicine and cell therapy due to their unique biological properties and therapeutic potential. The extraction of MSCs typically involves multiple processes, including tissue separation and filtration, chemical enzymatic hydrolysis, and centrifugal stratification.

[0003] However, existing extraction equipment can only complete a certain process, which means that multiple devices are required to complete the extraction process, resulting in complex and inefficient extraction operations. In addition, there is a risk of damaging cells during the transfer process between devices, thereby affecting subsequent experiments or treatment effects. Summary of the Invention

[0004] The purpose of the present invention is to overcome the above-mentioned shortcomings and provide a mesenchymal stem cell extraction device, so as to complete the tissue separation and filtration, chemical enzymatic hydrolysis and centrifugal stratification processes in a single extraction device, which will significantly improve the efficiency and quality of cell extraction and reduce the operation difficulty and extraction cost.

[0005] To achieve the above object, the specific solutions of the present invention are as follows:

[0006] A mesenchymal stem cell extraction device comprises an extraction body, wherein a motor and a clutch transmission mechanism connected to the motor are provided at the bottom center of the extraction body;

[0007] The clutch transmission mechanism includes a plurality of first friction blocks uniformly distributed along the circumferential direction and elastically slidably arranged along the radial direction;

[0008] A first turntable with a central hole is provided for rotation in the extraction body; a central shaft is provided at the top center of the first turntable and penetrates into the central hole; a vibration ring is provided for lifting movement in the first turntable, and a container unit is fixed to the vibration ring;

[0009] The central axis is rotatably sleeved with a second turntable, and the outer peripheral wall of the second turntable is provided with a first guide groove extending in the circumferential direction and having the same trajectory and a second guide groove with a smaller groove width than the first guide groove, the second guide groove being provided at the groove bottom position of the first guide groove and passing through the second turntable; the outer peripheral wall of the second turntable is provided with a downwardly inclined locking groove connected to the first guide groove corresponding to the wave crest position of each first guide groove; a plurality of second friction blocks elastically slidably arranged in the radial direction are uniformly distributed in the circumferential direction within the second turntable, and the second friction blocks are provided with an extrusion boss adapted to the wave crest position trajectory of the second guide groove; the first friction block and the second friction block both extend into the center hole;

[0010] A first bayonet is extended from the inner wall of the vibration ring and is movably embedded in the first guide groove. A second bayonet is elastically provided at the end of the first bayonet and is movably embedded in the second guide groove.

[0011] The present invention further includes a container unit including a container seat, a guide column penetrating downwardly through the vibration ring is provided at the bottom of the container seat; a guide cylinder is provided at the position of the first turntable corresponding to the container seat, the guide column movably extends into the guide cylinder and is connected to the first turntable by a tension spring.

[0012] The present invention further provides a limiting groove on the inner wall of the guide cylinder and a limiting platform on the outer wall of the guide column, wherein the limiting platform is movably embedded in the limiting groove.

[0013] The present invention further includes a container unit comprising a container body provided on a container seat and having a receiving groove, a container cover provided at an opening of the receiving groove, and a plurality of first semipermeable membranes and second semipermeable membranes uniformly distributed circumferentially in the receiving groove, wherein the first semipermeable membranes are provided above the corresponding second semipermeable membranes; a central cavity and an annular cavity located outside the central cavity are enclosed between the container body, the container cover, the first semipermeable membrane, and the second semipermeable membrane; a partition layer is provided in the annular cavity, which divides the annular cavity into an upper half cavity and a lower half cavity; a through hole is provided in the center of the container cover, and a filter is provided in the through hole.

[0014] Furthermore, in the present invention, two guide cylinders are arranged at intervals on the first turntable corresponding to each container seat, and two guide columns are arranged at intervals on each container seat.

[0015] The present invention further includes a clutch transmission mechanism that includes an active disk that is rotatably arranged at the bottom of the extraction body, the active disk is connected to the motor, and a plurality of radially arranged slide grooves are evenly distributed along the circumferential direction on the disk surface of the active disk, a centrifugal slider and a first spring are provided in the slide groove, the two ends of the first spring are connected between the centrifugal slider and the groove wall of the slide groove, and the first friction block is fixed on the centrifugal slider.

[0016] The present invention further provides a plurality of container units uniformly distributed along the circumference of the vibration ring; the inner wall of the vibration ring is provided with a first bayonet corresponding to each container unit, and a second bayonet is provided at the end of each first bayonet.

[0017] The present invention further provides the second turntable with an outer ring portion and an inner ring portion, a guide rod is provided between the inner ring portion and the outer ring portion corresponding to each second friction block, the second friction block is slidably sleeved on the guide rod, a second spring is connected between the second friction block and the outer ring portion, and the second spring is sleeved on the guide rod; the first guide groove and the second guide groove are provided on the outer ring portion.

[0018] Furthermore, in the present invention, each second friction block is connected to a stopper, an outer side wall of the stopper is provided with an extrusion boss, and both ends of the extrusion boss are provided with transition slopes.

[0019] Furthermore, the present invention provides an accommodating hole at the end of the first bayonet, a third spring is provided in the accommodating hole, and the second bayonet is movably inserted into the accommodating hole and abuts against the third spring.

[0020] The beneficial effects of the present invention are as follows: by controlling the motor speed, the present invention realizes the synchronous rotation and oscillation of the container unit at low speed, so that the cells and enzyme solution are fully mixed, and centrifugal stratification is achieved at high speed, thereby completing the tissue separation and filtration, chemical enzymatic hydrolysis and centrifugal stratification in a single extraction device, which will significantly improve the efficiency and quality of cell extraction and reduce the operation difficulty and extraction cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is a perspective view of the present invention;

[0022] Figure 2 1 is a cross-sectional schematic diagram of the present invention when the first friction block is in frictional contact with the central axis;

[0023] Figure 3 yes Figure 2 A partial enlarged schematic diagram of point A in the middle;

[0024] Figure 4 1 is a cross-sectional schematic diagram of the present invention when the second friction block contacts the hole wall of the central hole;

[0025] Figure 5 yes Figure 4 A partial enlarged schematic diagram of point B in the middle;

[0026] Figure 6 This is a schematic structural diagram of the present invention when the first bayonet enters the locking groove;

[0027] Figure 7 This is an exploded schematic diagram of the coordination of the first turntable, the vibration ring, and the container unit of the present invention;

[0028] Figure 8 yes Figure 7 A partial enlarged schematic diagram of point C in the middle;

[0029] Figure 9 yes Figure 7 A partial enlarged schematic diagram of point D in the middle;

[0030] Figure 10 It is a structural schematic diagram of the container unit of the present invention;

[0031] Figure 11 is a schematic cross-sectional view of a container unit of the present invention;

[0032] Figure 12 This is a schematic diagram of the structure of the cooperation between the second rotating disk, the second friction block, the stop block, and the second spring of the present invention;

[0033] Figure 13 It is a cross-sectional schematic diagram of the cooperation of the second rotating disk, the second friction block, the stop block, and the second spring of the present invention;

[0034] Figure 14 It is a structural schematic diagram of the present invention when the extrusion boss is embedded in the crest position of the second guide groove;

[0035] Figure 15 This is a schematic diagram of the structure of the cooperation between the second friction block, the stop block, and the second spring of the present invention;

[0036] Figure 16 It is a schematic diagram of the structure of the active disk, the centrifugal slider, the first spring, and the first friction block of the present invention;

[0037] Explanation of Reference Numerals: 1. Extraction body; 21. Motor; 22. Active disk; 221. Slide groove; 23. Centrifugal slider; 24. First spring; 25. First friction block; 31. First turntable; 311. Center hole; 312. Center axis; 313. Guide cylinder; 314. Limiting groove; 32. Vibrating ring; 321. First latch; 322. Second latch; 323. Third spring; 33. Container unit; 331. Container seat; 3311. Guide column; 3312, limit platform; 332, container body; 333, container cover; 334, first semipermeable membrane; 335, second semipermeable membrane; 336, partition layer; 337, filter; 34, tension spring; 41, second turntable; 411, first guide groove; 412, second guide groove; 413, locking groove; 414, outer ring; 415, inner ring; 416, guide rod; 42, second friction block; 43, stop block; 431, extrusion boss; 44, second spring. DETAILED DESCRIPTION

[0038] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments, but the scope of implementation of the present invention is not limited thereto.

[0039] like Figures 1 to 16 As shown, the mesenchymal stem cell extraction device described in this embodiment includes an extraction body 1. A motor 21 and a clutch transmission mechanism connected to the motor 21 are provided at the bottom center of the extraction body 1. The clutch transmission mechanism includes a plurality of first friction blocks 25 uniformly distributed along the circumference and elastically slidingly arranged along the radial direction.

[0040] A first turntable 31 with a center hole 311 is rotatably provided in the extraction body 1; a center shaft 312 is provided at the top center of the first turntable 31 and penetrates into the center hole 311; a vibration ring 32 is provided in the first turntable 31 for lifting and lowering, and a container unit 33 is fixed to the vibration ring 32;

[0041] A second rotary disk 41 is rotatably sleeved on the central shaft 312. The outer peripheral wall of the second rotary disk 41 is provided with a first guide groove 411 extending in the circumferential direction and having the same trajectory, and a second guide groove 412 with a smaller groove width than the first guide groove 411. The second guide groove 412 is provided at the groove bottom position of the first guide groove 411 and passes through the second rotary disk 41. The outer peripheral wall of the second rotary disk 41 is provided with a downwardly inclined locking groove 413 connected to the first guide groove 411 corresponding to the peak position of each first guide groove 411. A plurality of second friction blocks 42 elastically slidably arranged in the radial direction are uniformly distributed in the circumferential direction inside the second rotary disk 41. The second friction blocks 42 are provided with an extrusion boss 431 adapted to the peak position trajectory of the second guide groove 412. The first friction blocks 25 and the first friction block 25 both extend into the central hole 311.

[0042] A first latch 321 extends from the inner wall of the vibration ring 32 and is movably embedded in the first guide groove 411 . A second latch 322 is elastically provided at the end of the first latch 321 and is movably embedded in the second guide groove 412 .

[0043] Specifically, in the mesenchymal stem cell extraction device of this embodiment, at the initial stage, each first friction block 25 is arranged around the central axis 312 and is in frictional contact with the outer wall of the central axis 312 under the action of elasticity. Figure 2 As shown; when in use, a certain amount of digestive enzyme (such as collagenase) is added to the container unit 33 in advance, and then a certain amount of tissue cells are extracted by the extractor and placed on the container unit 33, and then the motor 21 drives each first friction block 25 to rotate clockwise at a low speed. Since the speed of each first friction block 25 is relatively low at this time, each first friction block 25 still maintains friction contact with the central axis 312 under the action of elasticity, thereby clamping the central axis 312, realizing the transmission between the motor 21 and the first turntable 31, as shown Figure 2 As shown, the first turntable 31 is driven to rotate by friction, and the first turntable 31 drives the vibration ring 32 and the container unit 33 to rotate synchronously. The vibration ring 32 drives the first latch 321 and the second latch 322 to rotate. The first latch 321 moves along the trajectory of the first guide groove 411, and the second latch 322 moves along the trajectory of the second guide groove 412. As a result, the vibration ring 32 is synchronized with the first guide groove 411 and the second guide groove 412 to reciprocate up and down relative to the first turntable 31, thereby driving the container unit 33 to rotate and oscillate, so that the cells and enzyme solution in the container unit 33 are fully mixed, promoting uniform enzymatic hydrolysis of the tissue and cell release, so that the target stem cells can be more effectively separated in the subsequent high-speed centrifugation stage, greatly improving the efficiency of cell extraction and reducing the operation time. During the rotation of the vibration ring 32, due to the restriction of the second latch 322 and the second guide groove 412, the first latch 321 will not enter the locking groove 413;

[0044] After the enzymatic hydrolysis is completed, the speed of the motor 21 is increased, so that each first friction block 25 overcomes the elastic effect and slides outward under the action of centrifugal force. At this time, each first friction block 25 is disconnected from the central shaft 312, so that the transmission between the motor 21 and the first turntable 31 is disconnected, and the first turntable 31 stops rotating.

[0045] During the outward movement of the first friction block 25, the first friction block 25 slides outward until it comes into contact with the corresponding second friction block 42, thereby realizing the transmission connection between the motor 21 and the second turntable 41, that is, the motor 21 drives the second turntable 41 to rotate relative to the first turntable 31, and at the same time, the first friction block 25 pushes the second friction block 42 to overcome the elastic effect and slide synchronously, and the second friction block 42 drives the extrusion boss 431 to move outward until the extrusion boss 431 is embedded in the crest position of the second guide groove 412, as shown in FIG. Figure 14 As shown, as the second rotating disk 41 rotates, when the second detent 322 contacts the extrusion protrusion 431, the extrusion protrusion 431 retracts the second detent 322 into the first detent 321, that is, the second detent 322 and the second guide groove 412 release the restriction on the first detent 321. At this time, when the second rotating disk 41 rotates to make the position of the first detent 321 correspond to the locking groove 413, the first detent 321 enters the locking groove 413 along the trajectory of the locking groove 413, and the second detent 322 remains in the retracted state. Figure 4 As shown, at this time, the first turntable 31 and the second turntable 41 are locked, and the second friction block 42 is in frictional contact with the wall of the center hole 311, so that the transmission between the first turntable 31 and the second turntable 41 is more stable. The second turntable 41 drives the first turntable 31 to rotate synchronously at high speed through the first latch 321 and the second friction block 42, and the first turntable 31 drives the container unit 33 to rotate at high speed, so that the cells in the container unit 33 are centrifugally stratified under the action of centrifugal force.

[0046] This embodiment controls the speed of the motor 21 to achieve synchronous rotation and oscillation of the container unit 33 at low speed, so that the cells and enzyme solution are fully mixed, and centrifugal stratification is achieved at high speed, thereby completing tissue separation and filtration, chemical enzymatic hydrolysis and centrifugal stratification in a single extraction device, which will significantly improve the efficiency and quality of cell extraction and reduce the difficulty of operation and extraction cost.

[0047] like Figure 15 and Figure 16 As shown, in this embodiment, the first friction block 25 is in a fan-shaped ring shape, which allows for better frictional fit between the first friction block 25 and the outer wall of the central shaft 312, increasing the contact surface and thus ensuring more reliable power transmission. In this embodiment, the second friction block 42 is generally in a fan-shaped ring shape, which allows for better contact between the first friction block 25 and the second friction block 42, while also allowing for better fit between the second friction block 42 and the wall of the central hole 311, increasing the contact surface and ensuring reliable power transmission.

[0048] like Figure 2 、 Figures 6 to 11 As shown, in the mesenchymal stem cell extraction device of this embodiment, in some embodiments, the container unit 33 includes a container seat 331, and a guide column 3311 is provided at the bottom of the container seat 331 and downwardly penetrates the vibration ring 32; the first turntable 31 is provided with a guide cylinder 313 at a position corresponding to the container seat 331, and the guide column 3311 is movably extended into the guide cylinder 313 and is connected to the first turntable 31 by a tension spring 34. Specifically, when the motor 21 is rotating at a low speed, the first detent 321 moves along the trajectory of the first guide slot 411, thereby driving the vibrating ring 32 and the container unit 33 to oscillate. When the first detent 321 moves from the trough position to the peak position of the first guide slot 411, the vibrating ring 32 drives the container unit 33 upward, causing the tension spring 34 to be stretched. When the first detent 321 moves from the peak position to the trough position of the first guide slot 411, the vibrating ring 32 drives the container unit 33 downward, and the tension spring 34 recovers its deformation. Thus, as the first detent 321 rotates, driven by the first guide slot 411, the vibrating ring 32 and the container unit 33 continuously move up and down, causing the container unit 33 to rotate and oscillate synchronously, thereby fully mixing the cells and enzyme solution in the container unit 33. At the same time, the coordination of the guide cylinder 313 and the guide post 3311 makes the vibrating ring 32 and the container unit 33 more stable during the up and down movement.

[0049] like Figures 9 to 11 As shown, in some embodiments of the mesenchymal stem cell extraction device of this embodiment, a limiting groove 314 is provided on the inner wall of the guide cylinder 313, and a limiting platform 3312 is provided on the outer wall of the guide column 3311. The limiting platform 3312 is movably embedded in the limiting groove 314. In this embodiment, the limiting groove 314 and the limiting platform 3312 cooperate to limit the vertical movement of the container unit 33, preventing the container unit 33 from detaching from the guide cylinder 313.

[0050] Illustratively, two limit grooves 314 are symmetrically provided on the inner wall of the guide cylinder 313, and the two limit grooves 314 are located in the same radial direction. Correspondingly, two limit platforms 3312 are symmetrically provided on the outer wall of the guide column 3311, and the two limit platforms 3312 are located in the same radial direction; the two limit platforms 3312 are slidably connected in the two limit grooves 314 one by one, so that the movement of the vibration ring 32 and the container unit 33 is more stable.

[0051] like Figure 1 、 Figure 2 、 Figure 4 、 Figure 10 and Figure 11As shown, in the mesenchymal stem cell extraction device of this embodiment, in some embodiments, the container unit 33 also includes a container body 332 provided on the container seat 331 and having a receiving groove, a container cover 333 provided at the opening position of the receiving groove, and a plurality of first semipermeable membranes 334 and second semipermeable membranes 335 uniformly distributed in the receiving groove along the circumferential direction, and the first semipermeable membranes 334 are provided above the corresponding second semipermeable membranes 335; a central cavity and an annular cavity located outside the central cavity are enclosed between the container body 332, the container cover 333, the first semipermeable membrane 334 and the second semipermeable membrane 335; a partition layer 336 is provided in the annular cavity, and the partition layer 336 divides the annular cavity into an upper half cavity and a lower half cavity; a through hole is provided in the center of the container cover 333, and a filter screen 337 is provided in the through hole.

[0052] In actual use, a certain amount of digestive enzyme (such as collagenase) is added to the central cavity in advance, and then a certain amount of tissue cells are extracted by the extractor into the through hole of the container cover 333. The cells are left to stand for a period of time so that the filter 337 can filter the cell suspension into the central cavity to remove the remaining cell waste liquid, such as large cell clumps and impurities.

[0053] When the motor 21 runs at a low speed, the container unit 33 rotates and oscillates synchronously, so that the cells and enzyme solution in the central cavity are fully mixed; when the motor 21 runs at a high speed, the cells in the central cavity are centrifugally stratified under the action of centrifugal force and the first semipermeable membrane 334 and the second semipermeable membrane 335, and the stratified cells are distributed in the upper cavity and the lower cavity.

[0054] like Figure 7 、 Figure 10 and Figure 11 As shown, in some embodiments of the mesenchymal stem cell extraction device of this embodiment, two guide cylinders 313 are spaced apart from each container seat 331 on the first turntable 31, and two guide posts 3311 are spaced apart from each container seat 331. This arrangement makes the container units 33 and the vibration ring 32 more stable during movement, and enhances structural stability.

[0055] like Figure 2 、 Figure 4 and Figure 16 As shown, in the mesenchymal stem cell extraction device of this embodiment, in some embodiments, the clutch transmission mechanism includes a driving disk 22 rotatably arranged at the bottom of the extraction body 1, the driving disk 22 is connected to the motor 21, and a plurality of radially arranged slide grooves 221 are evenly distributed along the circumference on the disk surface of the driving disk 22. A centrifugal slider 23 and a first spring 24 are provided in the slide groove 221. The two ends of the first spring 24 are connected between the centrifugal slider 23 and the groove wall of the slide groove 221, and the first friction block 25 is fixed on the centrifugal slider 23.

[0056] Specifically, in this embodiment, the motor 21 drives the active disk 22 to rotate, and the active disk 22 drives each centrifugal slider 23 and the first friction block 25 to rotate. When the motor 21 is at a low speed, the centrifugal force exerted on the centrifugal slider 23 is insufficient to cause the centrifugal slider 23 to drive the first friction block 25 to slide outward to overcome the elastic force of the first spring 24. In this way, the first friction block 25 maintains friction contact with the central shaft 312 under the elastic force of the first spring 24, as shown in FIG. Figure 2 As shown, the transmission between the motor 21 and the first turntable 31 is realized; and after the speed of the motor 21 is increased, the centrifugal force exerted on the centrifugal slider 23 gradually increases, so that the centrifugal slider 23 drives the first friction block 25 to overcome the elastic force of the first spring 24 and slide outward, thereby causing the first friction block 25 to break away from the central axis 312, and the power transmission between the motor 21 and the first turntable 31 is cut off. As the centrifugal slider 23 moves outward, the first spring 24 is further compressed until the first friction block 25 contacts the second friction block 42, and the first friction block 25 and the second friction block 42 realize power. The motor 21 drives the second rotary disk 41 to rotate through the first friction block 25 and the second friction block 42. At the same time, the first friction block 25 pushes the second friction block 42 to drive the extrusion boss 431 to move outward until the extrusion boss 431 is embedded in the crest position of the second guide groove 412, so that the second bayonet 322 retracts into the first bayonet 321, so that the first bayonet 321 enters the locking groove 413 to realize the transmission between the first rotary disk 31 and the second rotary disk 41. In this way, after the first bayonet 321 enters the locking groove 413, the second friction block 42 is in friction contact with the hole wall of the center hole 311, as shown in FIG. Figure 4 As shown, transmission between the first turntable 31 and the second turntable 41 is achieved, and the second turntable 41 drives the first turntable 31 to rotate at high speed to perform centrifugal stratification processing.

[0057] like Figure 1 、 Figure 2 、 Figure 4 and Figure 7 As shown, in the mesenchymal stem cell extraction device of this embodiment, in some embodiments, a plurality of container units 33 are evenly distributed along the circumference of the vibration ring 32. The number of the container units 33 can be set according to actual design requirements, such as setting the number of the container units 33 to four; the inner wall of the vibration ring 32 is provided with a first latch 321 corresponding to each container unit 33, and the end of each first latch 321 is provided with a second latch 322, so as to ensure the stability of the up and down movement of the vibration ring 32.

[0058] like Figure 4 、 Figure 12 and Figure 13As shown, in the mesenchymal stem cell extraction device of this embodiment, in some embodiments, the second turntable 41 is provided with an outer ring portion 414 and an inner ring portion 415, and a guide rod 416 is provided between the inner ring portion 415 and the outer ring portion 414 corresponding to each second friction block 42, and the second friction block 42 is slidably sleeved on the guide rod 416, and a second spring 44 is connected between the second friction block 42 and the outer ring portion 414, and the second spring 44 is sleeved on the guide rod 416; the first guide groove 411 and the second guide groove 412 are provided on the outer ring portion 414.

[0059] In this embodiment, a guide rod 416 is provided to provide guidance and limitation for the second friction block 42. After the first friction block 25 contacts the second friction block 42, the first friction block 25 pushes the second friction block 42 to overcome the elastic force of the second spring 44 and slide outward along the guide rod 416, so that the second spring 44 is compressed until the extrusion boss 431 is embedded in the second guide groove 412, and the second friction block 42 is in friction contact with the hole wall of the center hole 311.

[0060] like Figure 15 As shown, in some embodiments of the mesenchymal stem cell extraction device of this embodiment, each second friction block 42 is connected to a stopper 43. The outer wall of the stopper 43 is provided with a compression boss 431, and both ends of the compression boss 431 are provided with transition slopes. In this embodiment, the provision of the stopper 43 facilitates the placement of the compression boss 431 and the alignment of the compression boss 431 with the second guide groove 412. As the second friction block 42 slides outward, it can smoothly engage the crest of the second guide groove 412. As the second detent 322 moves along the second guide groove 412 and gradually contacts the transition slope of the compression boss 431, it retracts into the first detent 321, releasing the restraint exerted by the second detent 322 and the second guide groove 412 on the vibration ring 32.

[0061] like Figure 8 As shown, in some embodiments of the mesenchymal stem cell extraction device of this embodiment, a receiving hole is provided at the end of the first latch 321, and a third spring 323 is disposed within the receiving hole. The second latch 322 is movably inserted into the receiving hole and abuts against the third spring 323. In this embodiment, the receiving hole is provided to facilitate the installation of the third spring 323 and the second latch 322. The third spring 323 is provided to maintain the tendency of the second latch 322 to extend outward, so that the second latch 322 can reliably engage with the second guide groove 412 at low rotational speeds.

[0062] The above is only a preferred embodiment of the present invention. Therefore, any equivalent changes or modifications made according to the structure, characteristics and principles described in the scope of the patent application of the present invention are included in the protection scope of the patent application of the present invention.

Claims

1. A mesenchymal stem cell extraction device, characterized in that: It includes an extraction body, wherein a motor and a clutch transmission mechanism connected to the motor are provided at the bottom center of the extraction body; The clutch transmission mechanism includes a plurality of first friction blocks uniformly distributed along the circumferential direction and elastically slidably arranged along the radial direction; A first turntable with a central hole is provided for rotation in the extraction body; a central shaft is provided at the top center of the first turntable and penetrates into the central hole; a vibration ring is provided for lifting movement in the first turntable, and a container unit is fixed to the vibration ring; The central axis is rotatably sleeved with a second turntable, and the outer peripheral wall of the second turntable is provided with a first guide groove extending in the circumferential direction and having the same trajectory and a second guide groove with a smaller groove width than the first guide groove, the second guide groove being provided at the groove bottom position of the first guide groove and passing through the second turntable; the outer peripheral wall of the second turntable is provided with a downwardly inclined locking groove connected to the first guide groove corresponding to the wave crest position of each first guide groove; a plurality of second friction blocks elastically slidably arranged in the radial direction are uniformly distributed in the circumferential direction within the second turntable, and the second friction blocks are provided with an extrusion boss adapted to the wave crest position trajectory of the second guide groove; the first friction block and the second friction block both extend into the center hole; A first bayonet is extended from the inner wall of the vibration ring and is movably embedded in the first guide groove. A second bayonet is elastically provided at the end of the first bayonet and is movably embedded in the second guide groove.

2. The mesenchymal stem cell extraction device according to claim 1, characterized in that: The container unit includes a container seat, and a guide column is provided at the bottom of the container seat and penetrates the vibration ring downward; a guide cylinder is provided at the position of the first turntable corresponding to the container seat, and the guide column is movably extended into the guide cylinder and is connected to the first turntable by a tension spring.

3. The mesenchymal stem cell extraction device according to claim 2, characterized in that: The inner wall of the guide cylinder is provided with a limiting groove, and the outer wall of the guide column is provided with a limiting platform, and the limiting platform is movably embedded in the limiting groove.

4. The mesenchymal stem cell extraction device according to claim 2, characterized in that: The container unit further includes a container body disposed on the container seat and having a receiving groove, a container cover disposed at an opening of the receiving groove, and a plurality of first semipermeable membranes and second semipermeable membranes uniformly distributed in the receiving groove along a circumferential direction, wherein the first semipermeable membranes are disposed above corresponding second semipermeable membranes; A central cavity and an annular cavity located outside the central cavity are enclosed between the container body, the container cover, the first semipermeable membrane and the second semipermeable membrane; a partition layer is provided in the annular cavity, which divides the annular cavity into an upper half cavity and a lower half cavity; a through hole is provided in the center of the container cover, and a filter is provided in the through hole.

5. The mesenchymal stem cell extraction device according to claim 2, characterized in that: Two guide cylinders are arranged at intervals on the first turntable corresponding to each container seat, and two guide columns are arranged at intervals on each container seat.

6. The mesenchymal stem cell extraction device according to claim 1, characterized in that: The clutch transmission mechanism includes an active disk that is rotatably arranged at the bottom of the extraction body. The active disk is connected to the motor. A plurality of radially arranged slide grooves are evenly distributed along the circumference on the disk surface of the active disk. A centrifugal slider and a first spring are provided in the slide groove. The two ends of the first spring are connected between the centrifugal slider and the groove wall of the slide groove. The first friction block is fixed on the centrifugal slider.

7. The mesenchymal stem cell extraction device according to claim 1, characterized in that: A plurality of container units are evenly distributed along the circumference of the vibration ring; a first bayonet is provided on the inner wall of the vibration ring corresponding to each container unit, and a second bayonet is provided at the end of each first bayonet.

8. The mesenchymal stem cell extraction device according to claim 1, characterized in that: The second turntable is provided with an outer ring portion and an inner ring portion, and a guide rod is provided between the inner ring portion and the outer ring portion corresponding to each second friction block, the second friction block is slidably sleeved on the guide rod, a second spring is connected between the second friction block and the outer ring portion, and the second spring is sleeved on the guide rod; the first guide groove and the second guide groove are provided on the outer ring portion.

9. The mesenchymal stem cell extraction device according to claim 8, characterized in that: Each second friction block is connected to a stopper, an outer side wall of the stopper is provided with an extrusion boss, and both ends of the extrusion boss are provided with transition inclined surfaces.

10. The mesenchymal stem cell extraction device according to claim 1, characterized in that: An accommodating hole is provided at the end of the first bayonet, a third spring is provided in the accommodating hole, and the second bayonet is movably inserted into the accommodating hole and abuts against the third spring.

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