Fermentation device for preparing chitosan oligosaccharide from chitosan

Abstract

The invention belongs to the technical field of chitosan oligosaccharide production and processing in biotechnology, and particularly relates to a fermentation device for preparing chitosan oligosaccharide from chitosan, which comprises a tank body, a feed port and a discharge port are mounted on the tank body, a motor is mounted on the tank body, and the motor drives a rotating shaft and stirring blades in the tank body; a fixed seat is mounted in the tank body, the rotating shaft is mounted between the fixed seat and the motor shaft, and a jacking part is arranged on the fixed seat; a plurality of groups of arranging rings are mounted in the tank body, the arranging rings are in contact with the inner wall of the tank body, a synchronous rod and a connecting rod are mounted on the rotating shaft, and the arranging rings are mounted on the connecting ring; the fermentation device is simple in structure, the stirring blades rotate and move up and down, raw materials are stirred up and down and left and right, the raw materials are uniform and prevented from sinking to the bottom, meanwhile, the raw materials near the inner wall of the tank body are stirred in cooperation with up-down movement of the arrangement ring, the inner wall of the tank body is fully cleaned during cleaning, and the cleaning effect and cleaning efficiency of the fermentation device are improved.

Description

Technical Field

[0001] The invention belongs to the technical field of biotechnology chitosan oligosaccharide production and processing, in particular to a fermentation device for preparing chitosan oligosaccharide from chitosan. Background Art

[0002] Chitosan oligosaccharide, also known as chitosan oligosaccharide or low-molecular-weight chitosan, is an oligosaccharide product obtained by degrading chitosan with a degree of polymerization between 2 and 20, and a molecular weight of ≤3200Da. It is a low-molecular-weight product with good water solubility, great functional effects, and high biological activity. Chitosan oligosaccharide is also used as an ingredient in some medicines. In existing processes, microbial fermentation is often used to prepare chitosan oligosaccharides, and for this purpose, corresponding fermentation equipment is required. In the pharmaceutical production process, chitosan oligosaccharide, as an ingredient in some medicines, requires the use of biotechnology fermentation to produce pharmaceutical ingredients for medical treatment. In the preparation and production of chitosan oligosaccharides in the prior art, materials need to be filled into a chitosan oligosaccharide fermentation tank, and the chitosan oligosaccharide materials are uniformly fermented in the fermentation tank to produce chitosan oligosaccharides. However, the overall fermentation efficiency of the chitosan oligosaccharide materials in the prior art chitosan oligosaccharide fermentation tank is not high. When a large amount of materials is filled in the fermentation tank, a long period of precipitation and stirring is required to allow the materials to merge with each other, and the production efficiency cannot be improved. In addition, the materials are prone to unevenness and sinking to the bottom, which is not conducive to production. At the same time, after the fermentation tank produces chitosan oligosaccharides, the inner wall of the fermentation tank will be covered with residual chitosan oligosaccharides, which is very inconvenient to clean the fermentation tank. Summary of the Invention

[0003] In order to make up for the shortcomings of the existing technology, the stirring blade is rotated and moved up and down to stir the raw materials up and down and left and right to make the raw materials uniform and prevent the raw materials from sinking to the bottom. At the same time, the up and down movement of the sorting ring is coordinated to stir the raw materials near the inner wall of the tank, and the inner wall of the tank is fully cleaned during cleaning, thereby improving the cleaning effect and cleaning efficiency of the fermentation device. The present invention proposes a fermentation device for preparing chitosan oligosaccharides from chitosan.

[0004] The technical solution adopted by the present invention to solve the technical problem is as follows: the fermentation device for preparing chitosan oligosaccharides from chitosan of the present invention comprises a tank body, the tank body is equipped with a feed port and a discharge port, the tank body is equipped with a motor, the motor drives a rotating shaft in the tank body, and the rotating shaft is equipped with a stirring blade; A fixing seat is installed on the bottom surface of the tank body, the lower end of the rotating shaft is installed on the fixing seat, the upper end of the rotating shaft and the motor shaft of the motor are connected to each other through a spline, a jacking portion is provided on the fixing seat, and a contact rod is installed on the lower end of the rotating shaft, and the contact rod is in contact with the upper end of the jacking portion; The tank body is provided with a plurality of arranging rings, the arranging rings contacting the inner wall of the tank body, the arranging rings are connected to each other by a connecting rod, the upper end of the rotating shaft is provided with a driving block, the upper end of the rotating shaft is provided with a synchronizing rod slidably mounted, the driving block is located below the synchronizing rod, and the synchronizing rod is connected to the upper end of the connecting rod; The synchronization rod, the connection rod and the finishing ring are provided with interconnected cavities. An input pipe is installed on the tank body. The input pipe is connected with the cavity. The finishing ring is provided with a discharge hole.

[0005] Preferably, the distance between adjacent arranging rings in the tank body is smaller than the height of the jacking portion on the fixing seat, and the discharge holes on adjacent arranging rings in the tank body are installed in a staggered manner.

[0006] Preferably, a connecting sleeve is installed on the connecting rod, and the sorting ring is composed of at least two sub-units. A sliding tube is provided at the end face of the sub-unit, and the sliding tube is slidably installed in the connecting sleeve. A spring is installed in the sliding sleeve, and both ends of the spring are respectively connected to the end face of the sliding tube.

[0007] Preferably, the surface of the arranging ring is covered with an elastic layer, the surface of the elastic layer is provided with ejection holes, and the ejection holes on the elastic layer and the discharge holes on the arranging ring are offset from each other.

[0008] Preferably, the outlet directions of the ejection holes on the elastic layer are tilted toward the inner wall of the tank body, and the ejection holes are symmetrically distributed with the horizontal plane where the tidying ring is located as the center.

[0009] Preferably, a connecting ring area is provided between the elastic layer and the arranging ring, the connecting ring area is evenly distributed on the elastic layer, and the elastic layer is fixedly connected to the arranging ring only at the location of the connecting ring area.

[0010] Preferably, a ventilation groove is provided on the surface of the arranging ring, and the outlet of the discharge hole on the arranging ring is located in the ventilation groove.

[0011] Preferably, a roller is installed at the lower end of the rotating shaft, and the roller contacts the surface of the jacking portion; A suction bag is installed on the surface of the fixing seat, and the roller squeezes the suction bag. The suction bag is provided with an opening inclined downward.

[0012] The beneficial effects of the present invention are as follows: 1. The fermentation device for preparing chitosan oligosaccharides from chitosan described in the present invention is provided with a motor shaft, a rotating shaft, a fixing seat, a lifting part, a driving block, a synchronization rod, a connecting rod and a sorting ring, so that the stirring blade is driven by the motor to stir the raw materials up and down and left and right in the tank body, thereby promoting the circulation of the raw materials in the tank body. At the same time, the sorting ring can fully contact the inner wall of the tank body when moving up and down, thereby stirring the raw materials close to the inner wall of the tank body during fermentation, preventing the raw materials from sticking to and affecting the fermentation effect, and fully cleaning the inner wall of the tank body during cleaning, thereby improving the cleaning effect and cleaning efficiency.

[0013] 2. The fermentation device for preparing chitosan oligosaccharides from chitosan described in the present invention is provided with a sorting ring, a connecting sleeve, a sliding tube and a spring. The spring causes the sorting ring to have a tendency to expand in diameter, so that the sorting ring is tightly attached to the inner wall of the tank, thereby improving the cleaning effect of the inner wall of the tank and the stirring effect of the raw materials near the inner wall of the tank.

[0014] 3. The chitosan fermentation device for producing chitooligosaccharides of the present invention comprises a finishing ring, an elastic layer, a spray hole, a vent groove, and a discharge hole. The discharge hole is sealed by the elastic layer when not in use, preventing the ingress of raw materials. Furthermore, the ingress of sterile air or clean water in pulses causes the elastic layer to continuously expand and contract, creating an oscillating effect. This further prevents residual raw materials from remaining on the surface of the elastic layer, which could affect the cleaning efficiency of the fermentation device. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The present invention will be further described below with reference to the accompanying drawings.

[0016] Figure 1 is a perspective view of the fermentation apparatus of the present invention; Figure 2 It is a schematic structural diagram of the fermentation device of the present invention; Figure 3 It is a schematic structural diagram of the rotating shaft and stirring blades in the fermentation device of the present invention; Figure 4 It is a schematic structural diagram of a fixed seat and a roller in the fermentation device of the present invention; Figure 5 Schematic diagram of the installation position of a single finishing ring in the fermentation device of the present invention; Figure 6 It is a schematic structural diagram of the finishing ring in the fermentation device of the present invention; Figure 7 is a partial cross-sectional view of a finishing ring in the fermentation device of the present invention; Figure 8 yes Figure 3 A partial enlarged view of the middle part; Figure 9 yes Figure 5 A partial enlarged view of point B in the middle; Figure 10 yes Figure 7 A partial enlarged view of point C in the middle; In the figure: tank body 1, feed port 11, discharge port 12, input pipe 13, motor 14, motor shaft 2, rotating shaft 21, driving block 211, stirring blade 22, roller 23, fixing base 3, lifting part 31, suction bag 32, sorting ring 4, connecting sleeve 41, sliding tube 42, spring 43, elastic layer 44, ejection hole 441, ventilation groove 45, discharge hole 451, synchronization rod 5, connecting rod 51. DETAILED DESCRIPTION

[0017] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the present invention is further described below in conjunction with specific implementation methods.

[0018] like Figures 1 to 10 As shown, the fermentation device for preparing chitosan oligosaccharides from chitosan of the present invention comprises a tank body 1, on which a feed port 11 and a discharge port 12 are installed, and a motor 14 is installed on the tank body 1, and the motor 14 drives a rotating shaft 21 in the tank body 1, and a stirring blade 22 is installed on the rotating shaft 21; A fixing seat 3 is installed on the bottom surface of the tank body 1, and the lower end of the rotating shaft 21 is installed on the fixing seat 3. The upper end of the rotating shaft 21 is connected to the motor shaft 2 of the motor 14 via a spline. A lifting portion 31 is provided on the fixing seat 3, and a contact rod is installed at the lower end of the rotating shaft 21. The contact rod and the upper end of the lifting portion 31 are in contact with each other. A plurality of arranging rings 4 are installed in the tank body 1. The arranging rings 4 contact the inner wall of the tank body 1 and are connected to each other via a connecting rod 51. A driving block 211 is installed at the upper end of the rotating shaft 21. A synchronization rod 5 is slidably installed at the upper end of the rotating shaft 21. The driving block 211 is located below the synchronization rod 5. The synchronization rod 5 is connected to the upper end of the connecting rod 51. The synchronization rod 5, the connecting rod 51 and the finishing ring 4 define interconnected cavities. An input pipe 13 is installed on the tank body 1. The input pipe 13 is interconnected with the cavity. The finishing ring 4 defines a discharge hole 451. Among them, the outside of the tank body 1 in the fermentation device is equipped with a heating jacket in the prior art, so as to stably and accurately adjust the temperature inside the tank body 1 to ensure good results in the fermentation of chitosan to prepare chitosan oligosaccharides; During operation, the interior of the fermentation device is first sterilized by high-temperature steam, and then the chitosan raw material is added to the interior of the tank body 1 from the feed port 11. After that, the motor 14 is controlled by the controller to rotate, driving the rotating shaft 21 and the stirring blade 22 to rotate, stirring the raw materials in the tank body 1 to promote the mixing and uniform distribution of the raw materials. After that, after the fermentation is completed, the material is discharged from the discharge port 12, and the chitosan oligosaccharide product is separated; At the same time, since the rotating shaft 21 and the motor shaft 2 are connected by a spline, and the contact rod at the lower end of the rotating shaft 21 contacts the jacking portion 31 on the fixing seat 3, when the motor 14 drives the rotating shaft 21 and the stirring blade 22 to rotate, the contact rod on the rotating shaft 21 will periodically move from the surface of the fixing seat 3 to the surface of the jacking portion 31. Since the jacking portion 31 is higher than the surface of the fixing seat 3, the rotating shaft 21 and the stirring blade 22 are periodically moved up and down in the tank body 1, thereby cooperating with the rotation of the rotating shaft 21 to stir the stirring blade 22 up and down and left and right in the tank body 1, so that the stirring blade 22 stirs the raw materials in the tank body 1 as much as possible, avoiding the raw materials in the tank body 1 from sinking to the bottom and uneven mixing, thereby ensuring a good fermentation effect; At the same time, when the rotating shaft 21 is moved upward by the lifting portion 31, the driving block 211 on the rotating shaft 21 drives the synchronization rod 5, the connecting rod 51 and the finishing ring 4 to move upward. After that, after the contact rod passes over the lifting portion 31, the rotating shaft 21, the synchronization rod 5, the connecting rod 51 and the finishing ring 4 will fall again under the action of gravity, so that the finishing ring 4 will also move up and down periodically: During the fermentation process, the finishing ring 4 stirs the raw materials near the inner wall of the tank body 1, promotes the movement of the raw materials near the inner wall of the tank body 1, and prevents the raw materials near the inner wall of the tank body 1 from not being stirred in time and moving away during fermentation, which affects the fermentation effect. At the same time, the stirring and pushing of the raw materials near the inner wall by the finishing ring 4, in conjunction with the action of the stirring blades 22, promotes the circulation of the raw materials near the inner wall and the raw materials in the middle position of the tank body 1, thereby accelerating the transfer of external heat and making the temperature of the raw materials in the tank body 1 uniform and stable. At the same time, by stirring the raw materials near the inner wall of the tank body 1, it is prevented that the raw materials near the inner wall of the tank body 1 are kept close to the inner wall for a long time and do not move, causing the raw materials to adhere to the inner wall of the tank body 1. At the same time, when the finishing ring 4 moves up and down, sterile air can also be input from the outside through the input pipe 13 to further stir the raw materials near the inner wall of the tank body 1, and the input sterile air promotes the fermentation process. In the production process of chitosan oligosaccharides, the purpose of producing chitosan oligosaccharides is achieved by fermenting the raw materials by microorganisms using biological fermentation technology. This technology is fully applied in the current biotechnology used in medicine and plays a great role in the development of medical drug ingredients. After fermentation is completed, when cleaning the fermentation device: external cleaning water is sent into the sorting ring 4 from the input pipe 13, and sprayed onto the inner wall of the tank body 1 from the discharge hole 451 to clean the inner wall of the tank body 1, so as to avoid the presence of residual materials in the fermentation device and affect the subsequent use of the fermentation device. At the same time, through the up and down movement of the sorting ring 4, the inner wall of the tank body 1 is fully cleaned, thereby improving the cleaning effect and cleaning efficiency and reducing the difficulty of cleaning the residual raw materials in the tank body 1.

[0019] As an embodiment of the present invention, the distance between adjacent finishing rings 4 in the tank body 1 is smaller than the height of the jacking portion 31 on the fixing seat 3, and the discharge holes 451 on adjacent finishing rings 4 in the tank body 1 are staggered with each other. Since the distance between adjacent finishing rings 4 is less than the height of the jacking portion 31 on the fixing seat 3, when the rotating shaft 21 drives the finishing ring 4 to move up and down, there will be overlapping parts between the areas through which each finishing ring 4 moves, so that the finishing ring 4 can increase the coverage area when moving up and down as much as possible, avoiding the existence of dead corner areas on the inner wall of the tank body 1 that the finishing ring 4 cannot reach, resulting in areas on the inner wall of the tank body 1 that are not thoroughly cleaned with raw materials, affecting the cleaning effect and cleaning efficiency of the fermentation device. At the same time, since there is overlap between the moving areas of the finishing rings 4 when they move up and down, when the cleaning water sprayed from the discharge hole 451 flows downward along the inner wall of the tank body 1, the cleaning water will flush and clean the surface of the finishing ring 4, thereby avoiding the existence of residual raw materials on the surface of the finishing ring 4 itself; Furthermore, since the projections of the discharge holes 451 on the two adjacent sorting rings 4 on the horizontal plane are staggered with each other, the cleaning water sprayed from the discharge holes 451 can fully cover the inner wall of the tank body 1, effectively cleaning the raw materials remaining on the inner wall of the tank body 1, avoiding the residual raw materials after the inner wall of the tank body 1 is cleaned, thereby ensuring the subsequent normal use of the fermentation device.

[0020] As an embodiment of the present invention, a connecting sleeve 41 is installed on the connecting rod 51, and the finishing ring 4 is composed of at least two subunits. A sliding tube 42 is provided at the end surface of each subunit. The sliding tube 42 is slidably installed in the connecting sleeve 41. A spring 43 is installed in the sliding sleeve, and both ends of the spring 43 are respectively connected to the end surface of the sliding tube 42. Since the subunits of the sorting ring 4 are slidably installed in the connecting sleeve 41 through the sliding tube 42, the diameter of the sorting ring 4 can be adjusted as the sliding tube 42 moves in the connecting sleeve 41. Therefore, through the action of the spring 43, the sliding tubes 42 connected at both ends of the sliding sleeve are subjected to the elastic force of the spring 43 to move away from each other, thereby causing the diameter of the sorting ring 4 installed in the tank body 1 to tend to expand, so that the sorting ring 4 can be tightly attached to the inner wall of the tank body 1, thereby improving the stirring effect of the sorting ring 4 on the raw materials during fermentation and the cleaning effect of the raw materials remaining on the inner wall of the tank body 1 during cleaning.

[0021] As an embodiment of the present invention, the surface of the finishing ring 4 is covered with an elastic layer 44, and the surface of the elastic layer 44 is provided with a spray hole 441. The spray hole 441 on the elastic layer 44 and the discharge hole 451 on the finishing ring 4 are offset from each other. By covering the finishing ring 4 with an elastic layer 44 and staggering the ejection hole 441 and the discharge hole 451, when no sterile air or cleaning water is input into the finishing ring 4, the discharge hole 451 can be sealed by the elastic layer 44, thereby preventing the raw material in the tank body 1 from flowing back into the finishing ring 4 through the discharge hole 451 and affecting the normal use of the finishing ring 4. At the same time, since the arranging ring 4 and the inner wall of the tank body 1 are in contact with each other, the elastic layer 44 is covered on the surface of the arranging ring 4 to reduce the friction between the arranging ring 4 and the inner wall of the tank body 1, thereby reducing the wear of both. At the same time, due to the elasticity of the elastic layer 44, after the external sterile air or cleaning water is fed into the finishing ring 4 through the input pipe 13, the sterile air or cleaning water is discharged from the discharge hole 451 under the action of external pressure, and then enters the space between the elastic layer 44 and the finishing ring 4. At this time, the sterile air or cleaning water that enters will prop up the gap between the elastic layer 44 and the finishing ring 4, causing the elastic layer 44 to expand. Thereafter, the sterile air or cleaning water will be sprayed from the spray holes 441 on the elastic layer 44 onto the inner wall of the tank body 1, cleaning the raw materials remaining on the inner wall of the tank body 1 or stirring the raw materials near the inner wall of the tank body 1, thereby improving the cleaning effect or the stirring effect of the raw materials. At the same time, when the elastic layer 44 expands, it will further fully contact the inner wall of the tank body 1 and slightly deform the expanded elastic layer 44, thereby increasing the contact area between the elastic layer 44 and the inner wall of the tank body 1, thereby further improving the cleaning effect of the finishing ring 4 and the elastic layer 44 on the inner wall of the tank body 1 or increasing the stirring effect of the raw materials near the inner wall of the tank body 1, thereby promoting the circulation of the raw materials in the tank body 1; At the same time, sterile air or cleaning water is input from the outside in the form of pulses, causing the elastic layer 44 to continuously expand and contract, thereby causing the elastic layer 44 to produce an oscillating effect, promoting the detachment of raw materials attached to the surface of the elastic layer 44, reducing the possibility of residual raw materials on the surface of the elastic layer 44, and improving the cleaning effect of the fermentation device.

[0022] As an embodiment of the present invention, the outlet direction of the ejection holes 441 on the elastic layer 44 is tilted toward the inner wall of the tank body 1, and the ejection holes 441 are symmetrically distributed with the horizontal plane where the finishing ring 4 is located as the center; By setting the spray holes 441 symmetrically distributed above and below the horizontal plane, the sterile air or cleaning water sprayed from the spray holes 441 can fully reach the inner wall of the tank body 1 above and below the sorting ring 4, thereby improving the cleaning effect of the inner wall of the tank body 1. At the same time, since the sorting ring 4 will move up and down, the cleaning water or sterile air sprayed from the spray holes 441 onto the inner wall of the tank body 1 will come into contact with the surface of the connected sorting ring 4, and cooperate with the oscillation generated by the expansion and contraction of the elastic layer 44 on the sorting ring 4 to fully avoid the attachment of raw materials to the surface below the sorting ring 4 and close to the inner wall of the tank body 1, and the raw materials are not easy to clean, resulting in the residue of raw materials after the fermentation device is cleaned, affecting the subsequent normal use of the fermentation device.

[0023] As an embodiment of the present invention, a connecting ring area is provided between the elastic layer 44 and the finishing ring 4. The connecting ring area is evenly distributed on the elastic layer 44. The elastic layer 44 is fixedly connected to the finishing ring 4 only at the location of the connecting ring area. The gap formed between the elastic layer 44 and the finishing ring 4 is divided into a plurality of relatively separated spaces by the connecting ring area. Thus, when the elastic layer 44 expands, the elastic layer 44 is restricted and pulled at the location of the connecting ring area, that is, a concave ring is formed on the expanded elastic layer 44, thereby preventing the space after the expansion of the elastic layer 44 from being a whole, resulting in excessive expansion of the elastic layer 44 near the middle position of the subunit, causing damage to the elastic layer 44 or excessive force between the elastic layer 44 and the inner wall of the tank body 1 after expansion, affecting the normal use of the fermentation device.

[0024] As an embodiment of the present invention, a vent groove 45 is provided on the surface of the finishing ring 4 , and the outlet of the discharge hole 451 on the finishing ring 4 is located in the vent groove 45 ; Because the elastic layer 44 covers and adheres tightly to the surface of the sorting ring 4, the outlet of the discharge hole 451 is closed by the elastic layer 44, so that the sterile air or cleaning water in the discharge hole 451 pushes up the elastic layer 44, and the pressure required for the elastic layer 44 to expand is relatively large, resulting in the external sterile air or cleaning water requiring a greater pressure, increasing additional energy consumption. Therefore, a ventilation groove 45 is opened on the surface of the sorting ring 4, so that the outlet of the discharge hole 451 is located in the ventilation groove 45, and the spatial volume of the gap between the elastic layer 44 and the sorting ring 4 is additionally increased by the volume of the ventilation groove 45. When sterile air or cleaning water is filled in, the sterile air or cleaning water can first enter the ventilation groove 45, which facilitates its entry between the elastic layer 44 and the sorting ring 4 to expand the elastic layer 44, thereby reducing the pressure required for the external sterile air or cleaning water, and reducing the energy consumption required to input sterile air or cleaning water.

[0025] As an embodiment of the present invention, a roller 23 is installed at the lower end of the rotating shaft 21, and the roller 23 contacts the surface of the lifting portion 31; A suction bag 32 is installed on the surface of the fixing seat 3, and the roller 23 squeezes the suction bag 32. The suction bag 32 has an opening that is tilted downward. The installed roller 23 contacts the surface of the fixing base 3 and the surface of the top portion 31, thereby reducing wear on the contact rod, the surface of the fixing base 3, and the surface of the top portion 31, thereby extending the service life of the fermentation device. In addition, the rolling action of the roller 23 enables the rotating shaft 21 to move up and down smoothly under the action of the roller 23 and the top portion 31, thereby preventing the rotating shaft 21 from shaking or stuttering. At the same time, when the roller 23 moves along the surface of the fixed seat 3, the roller 23 will continuously squeeze the suction bag 32, so that the raw material entering the suction bag 32 is squeezed out, further stirring the material at the bottom of the tank body 1, reducing the possibility of the material sinking to the bottom.

[0026] The specific workflow is as follows: Among them, the outside of the tank body 1 in the fermentation device is equipped with a heating jacket in the prior art, so as to stably and accurately adjust the temperature inside the tank body 1; During operation, the interior of the fermentation device is first sterilized by high-temperature steam, and then the chitosan raw material is added to the interior of the tank body 1 from the feed port 11. After that, the motor 14 is controlled by the controller to rotate, driving the rotating shaft 21 and the stirring blade 22 to rotate, stirring the raw materials in the tank body 1 to promote the mixing and uniform distribution of the raw materials. After that, after the fermentation is completed, the material is discharged from the discharge port 12, and the chitosan oligosaccharide product is separated; At the same time, since the rotating shaft 21 and the motor shaft 2 are connected by a spline, and the contact rod at the lower end of the rotating shaft 21 contacts the top portion 31 on the fixing seat 3, when the motor 14 drives the rotating shaft 21 and the stirring blade 22 to rotate, the contact rod on the rotating shaft 21 will periodically move from the surface of the fixing seat 3 to the surface of the top portion 31, causing the rotating shaft 21 and the stirring blade 22 to periodically move up and down in the tank body 1, causing the stirring blade 22 to stir up and down and left and right in the tank body 1; During the fermentation process, the finishing ring 4 will stir the raw materials near the inner wall of the tank body 1, promoting the movement of the raw materials near the inner wall of the tank body 1. At the same time, when the finishing ring 4 moves up and down, sterile air can be introduced from the outside through the input pipe 13 to further stir the raw materials near the inner wall of the tank body 1 and promote the fermentation process. After fermentation is completed, when cleaning the fermentation device: external cleaning water is fed into the finishing ring 4 from the input pipe 13 and sprayed onto the inner wall of the tank body 1 from the discharge hole 451 to clean the inner wall of the tank body 1. At the same time, the inner wall of the tank body 1 is fully cleaned by the up and down movement of the finishing ring 4; Since the distance between adjacent finishing rings 4 is less than the height of the top portion 31 on the fixing seat 3, when the rotating shaft 21 drives the finishing rings 4 to move up and down, there will be overlapping parts between the areas through which the finishing rings 4 move. At the same time, when the cleaning water sprayed from the discharge hole 451 flows downward along the inner wall of the tank body 1, the cleaning water will flush and clean the surface of the finishing rings 4; Furthermore, since the projections of the discharge holes 451 on the two adjacent finishing rings 4 on the horizontal plane are offset from each other, the cleaning water sprayed from the discharge holes 451 can fully cover the inner wall of the tank body 1; The spring 43 acts to push the sliding tubes 42 connected at both ends of the sliding sleeve away from each other, thereby causing the diameter of the tidying ring 4 installed in the tank body 1 to expand, so that the tidying ring 4 can be tightly attached to the inner wall of the tank body 1. By covering the finishing ring 4 with an elastic layer 44 and staggering the ejection hole 441 and the discharge hole 451, when no sterile air or cleaning water is input into the finishing ring 4, the discharge hole 451 can be sealed by the elastic layer 44, thereby preventing the raw material in the tank body 1 from flowing back into the finishing ring 4 through the discharge hole 451. At the same time, since the arranging ring 4 and the inner wall of the tank body 1 are in contact with each other, the elastic layer 44 is covered on the surface of the arranging ring 4 to reduce the friction between the arranging ring 4 and the inner wall of the tank body 1; At the same time, due to the elasticity of the elastic layer 44, after the external sterile air or cleaning water is fed into the finishing ring 4 through the input pipe 13, the sterile air or cleaning water is discharged from the discharge hole 451 under the action of the external pressure and then enters the space between the elastic layer 44 and the finishing ring 4. At this time, the sterile air or cleaning water that enters will prop up the gap between the elastic layer 44 and the finishing ring 4, causing the elastic layer 44 to expand. Thereafter, the sterile air or cleaning water will be sprayed out from the spray hole 441 on the elastic layer 44 onto the inner wall of the tank body 1; At the same time, when the elastic layer 44 expands, the elastic layer 44 will further fully contact the inner wall of the tank body 1, and the expanded elastic layer 44 will be slightly deformed, thereby increasing the contact area between the elastic layer 44 and the inner wall of the tank body 1; At the same time, sterile air or clean water is input from the outside in the form of pulses, causing the elastic layer 44 to continuously expand and contract, thereby causing the elastic layer 44 to produce an oscillating effect, thereby promoting the detachment of the raw materials attached to the surface of the elastic layer 44; By providing the spray holes 441 symmetrically distributed in the horizontal plane, the sterile air or cleaning water sprayed from the spray holes 441 can fully reach the inner wall of the tank body 1 above and below the finishing ring 4. At the same time, since the finishing ring 4 moves up and down, the cleaning water or sterile air sprayed from the spray holes 441 onto the inner wall of the tank body 1 will contact the surface of the connected finishing ring 4. In combination with the oscillation generated by the expansion and contraction of the elastic layer 44 on the finishing ring 4, it is fully avoided that the raw materials are adhered to the surface below the finishing ring 4 and close to the inner wall of the tank body 1, and the raw materials are difficult to clean. The gap formed between the elastic layer 44 and the finishing ring 4 is divided into a plurality of relatively separated spaces by the connecting ring area. Thus, when the elastic layer 44 expands, the elastic layer 44 is restricted and pulled at the location of the connecting ring area, that is, a concave ring is formed on the expanded elastic layer 44, thereby preventing the space of the elastic layer 44 from being a whole after expansion, which would cause the elastic layer 44 near the middle of the subunit to expand excessively. A vent groove 45 is provided on the surface of the finishing ring 4, so that the outlet of the discharge hole 451 is located in the vent groove 45, thereby increasing the volume of the gap between the elastic layer 44 and the finishing ring 4 by the volume of the vent groove 45. When sterile air or clean water is filled in, the sterile air or clean water can first enter the vent groove 45, facilitating its entry between the elastic layer 44 and the finishing ring 4, causing the elastic layer 44 to expand, thereby reducing the pressure required to release the sterile air or clean water from the outside. The installed roller 23 contacts the surface of the fixing base 3 and the surface of the top portion 31, thereby reducing the wear on the contact rod, the surface of the fixing base 3 and the surface of the top portion 31, thereby extending the service life of the fermentation device; At the same time, when the roller 23 moves along the surface of the fixing seat 3, the roller 23 will continuously squeeze the suction bag 32, so that the raw material entering the suction bag 32 is squeezed out, and the material at the bottom of the tank body 1 is further stirred.

[0027] The basic principles, main features, and advantages of the present invention are shown and described above. Those skilled in the art should understand that the present invention is not limited to the foregoing embodiments. The foregoing embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and modifications may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and modifications are intended to fall within the scope of the present invention. The scope of protection claimed in the present invention is defined by the appended claims and their equivalents.

Claims

1. A fermentation device for preparing chitosan oligosaccharides from chitosan, comprising a tank body (1), wherein the tank body (1) is provided with a feed port (11) and a discharge port (12), and the tank body (1) is provided with a motor (14), wherein the motor (14) drives a rotating shaft (21) in the tank body (1), and a stirring blade (22) is provided on the rotating shaft (21); Its characteristics are: A fixing seat (3) is installed on the bottom surface of the tank body (1), the lower end of the rotating shaft (21) is installed on the fixing seat (3), the upper end of the rotating shaft (21) and the motor shaft (2) of the motor (14) are connected to each other through a spline, a lifting portion (31) is provided on the fixing seat (3), and a contact rod is installed on the lower end of the rotating shaft (21), and the contact rod and the upper end of the lifting portion (31) are in contact with each other; A plurality of arranging rings (4) are installed in the tank body (1), the arranging rings (4) contact the inner wall of the tank body (1), and the arranging rings (4) are connected to each other through a connecting rod (51). A driving block (211) is installed on the upper end of the rotating shaft (21), and a synchronization rod (5) is slidably installed on the upper end of the rotating shaft (21). The driving block (211) is located below the synchronization rod (5), and the synchronization rod (5) is connected to the upper end of the connecting rod (51); The synchronization rod (5), the connecting rod (51) and the arranging ring (4) are provided with interconnected cavities, an input pipe (13) is installed on the tank body (1), the input pipe (13) and the cavity are interconnected, and a discharge hole (451) is provided on the arranging ring (4).

2. The fermentation device for preparing chitosan oligosaccharides from chitosan according to claim 1, wherein: The distance between adjacent arranging rings (4) in the tank body (1) is less than the height of the jacking portion (31) on the fixing seat (3), and the discharge holes (451) on adjacent arranging rings (4) in the tank body (1) are installed in a staggered manner.

3. The fermentation device for preparing chitosan oligosaccharides from chitosan according to claim 1, wherein: A connecting sleeve (41) is installed on the connecting rod (51), and the arranging ring (4) is composed of at least two subunits. A sliding tube (42) is provided at the end surface of the subunit. The sliding tube (42) is slidably installed in the connecting sleeve (41). A spring (43) is installed in the sliding sleeve, and both ends of the spring (43) are respectively connected to the end surface of the sliding tube (42).

4. The fermentation device for preparing chitosan oligosaccharides from chitosan according to claim 1, wherein: The surface of the arranging ring (4) is covered with an elastic layer (44), and a spray hole (441) is provided on the surface of the elastic layer (44). The spray hole (441) on the elastic layer (44) and the discharge hole (451) on the arranging ring (4) are offset from each other.

5. The fermentation device for preparing chitosan oligosaccharides from chitosan according to claim 4, characterized in that: The outlet direction of the ejection holes (441) on the elastic layer (44) is tilted toward the inner wall of the tank body (1), and the ejection holes (441) are symmetrically distributed with the horizontal plane where the arranging ring (4) is located as the center.

6. The fermentation device for preparing chitosan oligosaccharides from chitosan according to claim 4, wherein: A connecting ring area is provided between the elastic layer (44) and the finishing ring (4), the connecting ring area is evenly distributed on the elastic layer (44), and the elastic layer (44) is fixedly connected to the finishing ring (4) only at the location of the connecting ring area.

7. A fermentation device for preparing chitosan oligosaccharides from chitosan according to claim 6, characterized in that: A vent groove (45) is provided on the surface of the arranging ring (4), and an outlet of the discharge hole (451) on the arranging ring (4) is located in the vent groove (45).

8. The fermentation device for preparing chitosan oligosaccharides from chitosan according to claim 1, characterized in that: A roller (23) is installed at the lower end of the rotating shaft (21), and the roller (23) contacts the surface of the jacking portion (31); A suction bag (32) is installed on the surface of the fixing seat (3), and the roller (23) squeezes the suction bag (32). The suction bag (32) is provided with an opening inclined downward.

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