Culture dish

Abstract

The culture dish comprises a bottom shell and an upper cover, the upper cover covers the bottom shell, a culture cavity is defined by the upper cover and the bottom shell, a culture medium is arranged at the bottom of the culture cavity, an interlayer cavity is formed in the upper cover, a plurality of balls are arranged in the interlayer cavity, and ball passing holes allowing the balls to pass through are formed in the lower end face of the upper cover. And the interlayer cavity is communicated with the culture cavity below the interlayer cavity through the ball passing hole. The culture dish provided by the invention is good in coating effect and simple and efficient to operate, and particularly, the upper cover and the bottom shell are in a covering state in the coating process, so that the risk of polluting a culture medium is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedicine-related equipment, in particular to a culture dish. Background Art

[0002] A culture dish is a laboratory vessel used for culturing microorganisms or cells. It consists of a flat bottom and a lid, and is generally made of glass or plastic. When a culture dish is needed, the culture medium and the microorganisms or cells to be cultured are placed in the culture dish, and then the microorganisms and cells are evenly spread with a coating rod, and finally the culture of various microorganisms and cells is completed. Among them, the coating work of the microorganisms or cells is completed by laboratory personnel using the existing coating rod, that is, the laboratory personnel take the coating rod and then use the coating rod to evenly spread the clumps of microorganisms or cells on the upper surface of the entire culture medium. This coating process not only has poor coating effect and low efficiency, but also the lid is opened for a long time during the coating process, increasing the risk of contaminating the culture medium. Summary of the Invention

[0003] The present invention aims to solve one of the technical problems in the related art to at least a certain extent: to provide a culture dish with good coating effect, simple and efficient operation, especially when the upper cover and the bottom shell are in a covering state during the coating process, thereby reducing the risk of contaminating the culture medium.

[0004] To this end, one object of the present invention is to provide a culture dish, which includes a bottom shell and an upper cover, wherein the upper cover is covered on the bottom shell and forms a culture chamber with the bottom shell, the bottom of the culture chamber is provided with a culture medium, the interior of the upper cover has an interlayer cavity, and the interlayer cavity is provided with a plurality of balls, and the lower end surface of the upper cover has a ball hole for allowing the balls to pass through, and the interlayer cavity is connected to the culture chamber below through the ball hole. By shaking the bottom shell, the balls can fall into the culture chamber through the ball hole under the action of inertia, and the rolling of the balls during the shaking of the bottom shell can be used to evenly coat the bacterial colony on the culture medium. Compared with the existing coating rod, the coating effect is better, the operation is simple and efficient, and the upper cover and the bottom shell remain in a covered state during the entire coating process, reducing the contamination of the culture chamber by the external environment.

[0005] According to an example of the present invention, a protective patch is provided on the lower end surface of the upper cover, and the protective patch is attached to the ball hole.

[0006] According to an example of the present invention, the ball hole is located on the lower end surface of the upper cover at a position away from the central axis, so that the ball hole is connected to the edge of the interlayer cavity.

[0007] According to an example of the present invention, a guide surface for driving the balls to move radially outward is provided on the bottom surface of the interlayer cavity. The guide surface can enable the balls to be better positioned at the edge of the interlayer cavity.

[0008] According to one embodiment of the present invention, the bottom surface of the interlayer chamber is provided with ridges and / or recesses on both sides of the ball hole along the circumferential direction. The ridges and / or recesses can reduce the risk of balls in the interlayer chamber falling through the ball hole into the culture chamber below when the bottom shell and the upper cover are used together for subsequent culture operations.

[0009] According to an example of the present invention, the upper cover includes an outer cover and an inner cover, the outer cover is covered outside the inner cover, the inner cover and the outer cover together form an interlayer cavity, and the ball hole is located on the inner cover.

[0010] According to an example of the present invention, the outer cover is sleeved outside the bottom shell, and the upper end of the bottom shell abuts against the inner cover. The outer cover and the inner cover are axially slidably matched. A blocking protrusion is provided on the outer cover at a position corresponding to the ball hole. The outer cover has an upward position and a downward position. When the outer cover is in the upward position, the distance L1 between the ball hole and the shielding protrusion is equal to or greater than the ball diameter; When the outer cover is in the downward position, the distance L2 between the ball hole and the shielding protrusion is smaller than the ball diameter. The upward or downward movement of the outer cover can control whether the ball can pass through the ball hole, effectively preventing the ball from accidentally falling into the culture chamber.

[0011] According to an example of the present invention, the outer side wall of the inner cover matches the inner side wall of the bottom shell, the upper end of the inner cover side wall has an outward-turned flange, and the outer side surface of the flange has an inward-concave annular groove, and the inner side wall of the outer cover has a positioning protrusion matching the annular groove, and the outer cover has an upward position where the positioning protrusion is assembled in the annular groove and a downward position where the positioning protrusion is located below the flange.

[0012] According to one example of the present invention, the outer cover is configured so that when the outer cover is in the upward position, the lower end surface of the outer cover is flush with the lower end surface of the inner cover. By flushing the lower end surface of the outer cover with the lower end surface of the inner cover, the outer cover is more conveniently in the upward position during assembly with the inner cover.

[0013] Another object of the present invention is to provide a method for using the above-mentioned culture dish, comprising the following steps: S1. Prepare a culture medium in a bottom shell and place the bacterial colony to be cultured on the culture medium; S2. Select an upper cover that matches the bottom shell, and tear off the patch attached to the ball hole to open the ball hole; S3. Close the upper cover on the bottom shell and shake the bottom shell and the upper cover horizontally so that the balls fall into the culture chamber through the ball holes and the bacteria are dispersed on the culture medium as the balls roll; S4, invert the bottom shell and the upper cover so that the ball returns to the interlayer cavity through the ball hole; S5, pressing the bottom shell and the upper cover tightly, so that the upper cover moves from the upper position to the lower position; S6. Reset the inverted bottom shell and upper cover.

[0014] The above technical solution has the following advantages or beneficial effects: First, after the upper cover is closed on the bottom shell, the balls only need to be shaken to make the balls automatically fall onto the culture medium, and as the shaking continues, the bacterial colony on the culture medium is coated by the rolling of the balls. After coating, the bacterial colony is more evenly dispersed, and its coating effect is better than the existing coating rod. Secondly, the upper cover and the bottom shell remain in a closed state during the entire coating process, thereby avoiding the problem of the upper cover being opened due to the coating operation and then contaminating the culture chamber; secondly, the ball hole is set at a position close to the side wall of the interlayer cavity, and by adding a guide surface, the balls can pass through the ball hole more quickly during the shaking of the bottom shell; finally, through the movable design between the inner cover and the outer cover, the outer cover has an upward position and a downward position, and then the blocking protrusion on the upper cover is used to prevent the balls from passing through the ball hole or allow the balls to pass through the ball hole.

[0015] Additional aspects and advantages of the present invention will be set forth in part in the description which follows and, in part, will be obvious from the description which follows, or may be learned by practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a structural schematic diagram of a culture dish of the present invention.

[0017] Figure 2 It is a schematic diagram of the explosive disassembly of the culture dish of the present invention.

[0018] Figure 3 for Figure 1 Schematic diagram of the internal structure of the culture dish in longitudinal section.

[0019] Figure 4 for Figure 3 A partial enlarged schematic diagram of the "A" area when the middle and outer covers are in the downward position.

[0020] Figure 5 for Figure 3 A partial enlarged schematic diagram of the "A" area when the middle and outer covers are in the upward position.

[0021] Figure 6 It is a flow chart of the method for using the culture dish of the present invention.

[0022] Among them, 1. bottom shell; 2. outer cover; 3. inner cover; 4. culture chamber; 5. culture medium; 6. interlayer chamber; 7. ball; 8. ball hole; 9. protective patch; 10. guide surface; 11. ridge; 12. shielding protrusion; 13. flange; 14. annular groove; 15. positioning protrusion. DETAILED DESCRIPTION

[0023] The following describes embodiments of the present invention in detail, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and are not to be construed as limiting the present invention.

[0024] The culture dish according to an embodiment of the present invention will be described in detail below with reference to the accompanying drawings.

[0025] The present invention provides a culture dish, as shown in the figure, which includes a bottom shell 1 and an upper cover. The top of the bottom shell is an open opening. The upper cover is covered on the bottom shell 1 from top to bottom and forms a culture chamber 4 between the bottom shell 1 and the bottom shell 1. The bottom of the culture chamber 4 is paved with a culture medium 5, which is preferably a solid culture medium 5. The interior of the upper cover has an interlayer cavity 6, and a plurality of balls 7 are arranged in the interlayer cavity 6. The lower end surface of the upper cover has a ball hole 8, which is connected to the interlayer cavity 6 and is configured to allow the balls 7 to pass through, that is, the aperture of the ball hole 8 is larger than the diameter of the balls 7. At this time, Figure 3 As shown, the culture chamber 4 is located below the interlayer chamber 6, and the interlayer chamber 6 is connected to the culture chamber 4 below through the ball hole 8. In this embodiment, when the bottom shell 1 and the upper cover are in the upright position with the bottom shell 1 at the bottom and the upper cover at the top, shaking the bottom shell 1 can allow the balls 7 to fall into the culture chamber 4 through the ball hole 8, and then the rolling of the balls 7 on the culture medium 5 can drive the bacteria or cells on the surface of the culture medium 5 to be dispersed, achieving a uniform coating effect. After the coating is completed, it is only necessary to invert the bottom shell 1 and the upper cover to the inverted position, that is, the upper cover is at the bottom and the bottom shell 1 is at the top. At this time, the balls 7 can return to the interlayer chamber 6 through the ball hole 8 under the action of their own gravity, completing the separation of the balls 7 and the culture medium 5.

[0026] After the uniform distribution action is completed in the above embodiment, the upper cover together with the balls 7 returned to the interlayer cavity 6 can be removed from the bottom shell 1, and then a new existing culture dish cover can be replaced. At the same time, the used upper cover and balls 7 can be cleaned, sterilized, and disinfected before reuse; the upper cover and the bottom shell 1 can also be used together for subsequent culture work. At this time, the balls 7 are located in the interlayer cavity 6, which has little effect on the culture of the bacterial flora in the culture cavity, or even no effect.

[0027] Based on the preferred embodiment described above, a protective patch 9 is provided on the lower end surface of the upper cover, and the protective patch 9 is attached to the ball hole 8. Before the upper cover is used, the protective patch 9 can effectively block the ball hole 8, on the one hand, preventing the balls 7 in the interlayer cavity 6 from falling out, and on the other hand, reducing the impact of the external environment on the balls 7 in the interlayer cavity 6, keeping the balls 7 clean.

[0028] Based on the preference of the above embodiment, the ball hole 8 is located on the lower end surface of the upper cover away from the central axis, so that the ball hole 8 is connected to the edge of the interlayer cavity 6. In this embodiment, when the bottom shell 1 is shaken and the upper cover and the balls 7 inside the upper cover are shaken, the balls 7 can fall out of the ball hole 8 more easily after being close to the edge. Preferably, the cross section of the interlayer cavity 6 is circular. At this time, when the bottom shell 1 is shaken in the circumferential direction, the balls 7 will cling to the inner wall of the interlayer cavity 6 under the action of centrifugal force. At this time, as the balls 7 move in the circumferential direction, each ball 7 can fall into the culture cavity 4 below in sequence when passing above the ball hole 8.

[0029] Further, if Figure 2 and Figure 3 As shown, the bottom surface of the interlayer cavity 6 has a guide surface 10 that is higher in the middle and lower around the periphery, which is used to drive the balls 7 to move radially outward. In this embodiment, when the bottom shell 1 is placed on a horizontal surface, the balls 7 in the interlayer cavity 6 can automatically move radially outward under the action of the guide surface 10 and the weight of the balls 7 until the balls 7 abut against the inner wall of the interlayer cavity 6.

[0030] In the above embodiment, after the balls 7 have completed the uniform coating work in the culture chamber 4, the balls 7 can be returned to the interlayer chamber 6 by inverting the bottom shell 1 and the upper cover. If the bottom shell 1 and the upper cover are further used for subsequent culture work, there is a risk that the balls 7 will fall back into the culture chamber, affecting the culture of the bacterial colony on the culture medium. Therefore, the improvement of this embodiment is as follows: Figure 2 and Figure 3 As shown, the bottom surface of the interlayer cavity 6 is provided with ridges 11 and / or recesses, which are arranged circumferentially on both sides of the position of the ball hole 8. The ridges 11 and / or recesses can hinder the rolling of the ball 7. During the culture process, when the personnel slightly translate the culture dish, the ball 7 will promptly roll in the interlayer cavity 6. When the ball 7 approaches the ball hole 8 along the circumferential direction, it will first hit the ridges 11 or recesses. The ridges 11 can abut against the ball 7 to prevent it from moving further toward the ball hole 8, and the recesses can allow the bottom of the ball 7 to sink into the recesses, thereby limiting the further movement of the ball 7 toward the ball hole 8. It should be understood that when the shell 1 is shaken to a large extent, the ball 7 can pass over the above-mentioned ridges 11 and / or pits under the action of a large inertial force. The purpose of the ridges 11 and / or pits in this embodiment is to prevent the ball 7 from approaching the ball hole 8 when the bottom shell 1 is placed horizontally or when the shell 1 is slightly tilted or slightly translated.

[0031] See also Figure 2 and Figure 3As shown, the upper cover includes an outer cover 2 and an inner cover 3 which are detachably connected to each other, the outer cover 2 having a top plate and side plates surrounding the top plate, the outer cover 2 is covered on the outside of the inner cover 3, and the inner cover 3 and the outer cover 2 together form an interlayer cavity 6, that is, the inner cover 3, the top plate of the outer cover 2 and part of the side plates together form the interlayer cavity 6, and the ball hole 8 is located on the inner cover 3.

[0032] Preferably, the inner wall of the side plate of the outer cover 2 matches the outer wall of the bottom shell 1, so that the outer cover 2 can be put on the outside of the bottom shell 1 with the opening direction facing downward, and the upper end of the bottom shell 1 is against the inner cover 3, and the outer cover 2 and the inner cover 3 are axially slidably matched. A blocking protrusion 12 is provided on the outer cover 2 at a position corresponding to the ball hole 8, and the outer cover 2 has an upward position and a downward position.

[0033] See also Figure 3 and Figure 5 When the outer cover 2 is in the upward position, the distance L1 between the ball hole 8 and the shielding protrusion 12 is equal to or greater than the diameter of the ball 7. At this point, the ball 7 can pass through the passage between the ball hole 8 and the shielding protrusion 12, allowing the ball 7 in the interlayer cavity 6 to pass through the ball hole 8 and fall into the culture cavity 4. Alternatively, when the housing 1 is inverted, the ball 7 in the culture cavity 4 that has completed the coating process can return to the interlayer cavity 6 through the ball hole 8 and the passage between the ball hole 8 and the shielding protrusion 12.

[0034] See also Figure 3 and Figure 4 When the outer cover 2 is in the downward position, the distance L2 between the ball hole 8 and the shielding protrusion 12 is smaller than the diameter of the ball 7. At this time, the ball 7 in the interlayer cavity 6 can be confined in the interlayer cavity 6 and will not fall out of the ball hole 8.

[0035] Based on one of the preferred examples in the above embodiment where the outer cover 2 has an upward position and a downward position There is sliding friction between the outer wall of the inner cover 3 and the inner wall of the outer cover 2 , and the friction resistance of the sliding friction is greater than the sum of the weight of the inner cover 3 and the weight of all the balls 7 .

[0036] Based on the second preferred example of the above embodiment in which the outer cover 2 has an upward position and a downward position join Figure 4 and Figure 5As shown, the outer side wall of the inner cover 3 matches the inner side wall of the bottom shell 1, the upper end of the side wall of the inner cover 3 has an outward-turned flange 13, and the outer side surface of the flange 13 has an inward-concave annular groove 14, and the inner side wall of the outer cover 2 has a positioning protrusion 15 matching the annular groove 14, and the outer cover 2 has an upward position and a downward position, wherein when the outer cover 2 is in the upward position, the positioning protrusion 15 is assembled in the annular groove 14, and at the same time, the distance L1 between the ball hole 8 and the shielding protrusion 12 is equal to or greater than the diameter of the ball 7; wherein when the outer cover 2 is in the downward position, the positioning protrusion 15 is located below the flange 13, and at this time, the distance L2 between the ball hole 8 and the shielding protrusion 12 is less than the diameter of the ball 7.

[0037] Preferably, the outer cover 2 and bottom shell 1 are made of glass, and the inner cover 3 is made of plastic. The flange 13 of the inner cover 3 can elastically deform to allow the annular groove 14 to engage with the positioning protrusion 15, or the flange 13 to be positioned above the positioning protrusion 15 when the positioning protrusion 15 descends to the lowered position along with the outer cover 2. The plastic material is a conventional material used in existing culture dishes, and the specific composition of the plastic material is not described in detail in this embodiment.

[0038] In the above embodiment, when the upper cover and the ball 7 in the upper cover are reusable, the outer cover 2, the inner cover 3 and the ball 7 need to be reassembled after completing all the steps of cleaning, disinfection and sterilization, and then be reused. The newly assembled upper cover needs to have the outer cover 2 in a Figure 5 The upward position shown, i.e., the positioning protruding ring 15 is assembled in the annular groove 14. To this end, the improvement of this embodiment is that the outer cover 2 is arranged so that the lower end surface of the outer cover 2 is flush with the lower end surface of the inner cover 3 when the outer cover 2 is in the upward position. In this embodiment, with the aid of a clean work surface or other flat clean surface, the outer cover 2 is first placed over the inner cover 3, and then a thrust is applied to the outer cover 2, so that the lower end surface of the inner cover 3 abuts the above-mentioned work surface until the lower end surface of the outer cover 2 is flush with the lower end surface of the inner cover 3. At this time, the positioning protruding ring 15 is embedded in the annular groove 14, and the outer cover 2 is in the upward position relative to the inner cover 3.

[0039] Based on the above embodiments of the culture dish, the present invention provides a method for using the culture dish, comprising the following steps: S1, prepare a culture medium 5 in the bottom shell 1, and place the bacterial colony to be cultured on the culture medium 5; S2. Select an upper cover that matches the bottom shell 1 and tear off the protective patch 9 on the bottom of the upper cover, that is, tear off the protective patch 9 attached to the ball hole 8; S3. Place the upper cover with its opening facing downward on the bottom shell 1 and shake the bottom shell 1 and the upper cover horizontally so that the balls 7 pass through the ball holes 8 and fall into the culture chamber 4. As the balls 7 roll, the bacterial colonies are dispersed on the upper surface of the culture medium 5. S4, invert the bottom shell 1 and the upper cover so that the ball 7 falls back into the interlayer cavity 6 through the ball hole 8; S5, pressing the bottom shell 1 and the upper cover, so that the upper cover moves from the upper position to the lower position; S6, resetting the inverted bottom shell 1 and upper cover.

[0040] The time for the balls 7 to be shaken in the culture chamber 4 for coating in step S3 can be obtained through a limited number of tests.

[0041] The above inversion means Figure 3 The up and down directions shown are reversed 180 degrees, so that the bottom shell 1 is on top and the upper cover is on the bottom. Figure 3 In the position shown, the bottom shell 1 is at the bottom and the upper cover is at the top.

[0042] The above-mentioned pressing of the bottom shell 1 and the upper cover refers to applying a force to the bottom shell 1 and / or the upper cover to drive the bottom shell 1 and the upper cover to move relative to each other.

[0043] Although the embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention.

[0044] Various changes and modifications will undoubtedly become apparent to those skilled in the art upon reading the foregoing description. Therefore, the appended claims should be construed to encompass all changes and modifications within the true intent and scope of the present invention. Any and all equivalents within the scope of the claims should be considered to be within the intent and scope of the present invention.

Claims

1. A culture dish comprising a bottom shell (1) and an upper cover, wherein the upper cover is covered on the bottom shell (1) and encloses a culture cavity (4) with the bottom shell (1), and a culture medium (5) is provided at the bottom of the culture cavity (4), characterized in that: The upper cover has an interlayer cavity (6) inside, and a plurality of balls (7) are arranged in the interlayer cavity (6). The lower end surface of the upper cover has a ball hole (8) for allowing the balls (7) to pass through, and the interlayer cavity (6) is connected to the culture cavity (4) below through the ball hole (8).

2. The culture dish according to claim 1, wherein: A protective patch (9) is provided on the lower end surface of the upper cover, and the protective patch (9) is attached to the ball hole (8).

3. The culture dish according to claim 2, wherein: The ball hole (8) is located on the lower end surface of the upper cover at a position away from the central axis, so that the ball hole (8) is connected to the edge of the interlayer cavity (6).

4. The culture dish according to claim 3, wherein: The bottom surface of the interlayer cavity (6) is provided with a guide surface (10) for driving the balls (7) to move radially outward.

5. The culture dish according to claim 4, characterized in that: The bottom surface of the interlayer cavity (6) is provided with convex ridges (11) and / or concave pits on both sides of the ball hole (8) along the circumferential direction.

6. The culture dish according to any one of claims 2 to 5, characterized in that: The upper cover comprises an outer cover (2) and an inner cover (3), wherein the outer cover (2) covers the outside of the inner cover (3), and the inner cover (3) and the outer cover (2) together form an interlayer cavity (6), and the ball hole (8) is located on the inner cover (3).

7. The culture dish according to claim 6, wherein: The outer cover (2) is sleeved on the outside of the bottom shell (1), and the upper end of the bottom shell (1) is in contact with the inner cover (3). The outer cover (2) and the inner cover (3) are axially slidably matched. A blocking protrusion (12) is provided on the outer cover (2) at a position corresponding to the ball hole (8). The outer cover (2) has an upward position and a downward position. When the outer cover (2) is in the upward position, the distance L1 between the ball hole (8) and the shielding protrusion (12) is equal to or greater than the diameter of the ball (7); When the outer cover (2) is in the downward position, the distance L2 between the ball hole (8) and the shielding protrusion (12) is smaller than the diameter of the ball (7).

8. The culture dish according to claim 7, wherein: The outer side wall of the inner cover (3) matches the inner side wall of the bottom shell (1), the upper end of the side wall of the inner cover (3) has an outward-turned flange (13), and the outer side surface of the flange (13) has an inward-concave annular groove (14), and the inner side wall of the outer cover (2) has a positioning protruding ring (15) matching the annular groove (14), and the outer cover (2) has an upward position in which the positioning protruding ring (15) is assembled in the annular groove (14) and a downward position in which the positioning protruding ring (15) is located below the flange (13).

9. The culture dish according to claim 8, characterized in that: The outer cover (2) is arranged such that when the outer cover (2) is in an upward position, the lower end surface of the outer cover (2) is flush with the lower end surface of the inner cover (3).

10. A method for using the culture dish according to claims 7-9, characterized in that: The following steps are included S1. Prepare a culture medium (5) in the bottom shell (1), and place the bacterial colony to be cultured on the culture medium (5); S2. Tear off the protective patch (9) at the bottom of the upper cover to open the ball hole (8); S3, closing the upper cover on the bottom shell (1), and shaking the bottom shell (1) and the upper cover in the horizontal direction, so that the ball (7) passes through the ball hole (8) and falls into the culture chamber (4), and as the ball (7) rolls, the bacterial colony is dispersed on the culture medium (5); S4, inverting the bottom shell (1) and the upper cover so that the ball (7) passes through the ball hole (8) and returns to the interlayer cavity (6); S5, pressing the bottom shell (1) and the upper cover, so that the upper cover moves from the upper position to the lower position; S6, resetting the inverted bottom shell (1) and the upper cover.

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