Pathogen detection device and method

Abstract

The invention provides a pathogen detection device and a pathogen detection method, belongs to the technical field of medical instruments, and aims to solve the technical problems that pathogen detection needs long time and a special experimental environment is needed in the prior art. The invention provides a pathogen detection device and method. Wherein the pathogen detection device comprises a motor, a sample box, a clear liquid mechanism and a filling mechanism; the bottom of the sample box is detachably connected to an output shaft of the motor, and the clear liquid mechanism and the filling mechanism are arranged in the upper area of the sample box and used for extracting substances from the sample box and adding substances into the sample box respectively; a round-bottom containing cavity is formed in the sample box, a ventilation channel is arranged at the bottom of the containing cavity, an elastic sealing film and a rubber plug are arranged at the top of the containing cavity, the rubber plug presses the edge of the elastic sealing film to an opening of the containing cavity, a sample box body is made of metal materials, and an electromagnetic induction coil is arranged on the periphery of the sample box body. The method does not need a special laboratory environment, can quickly complete pathogen detection, and solves the problems of long detection time and need of a special environment in the prior art.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a pathogen detection device and method. Background Art

[0002] Pathogens refer to pathogenic factors that can cause infectious diseases in the host, including bacteria, viruses, fungi, mycoplasmas, chlamydia, etc. They are widely present in the natural environment and in organisms, and are the root cause of many infectious diseases.

[0003] Currently, the main method for detecting pathogens is pathogen culture, which simulates the pathogen's growth environment to cause it to proliferate, thereby determining its presence. However, this method is time-consuming and requires specialized laboratory equipment and a professional operating environment, making it difficult to quickly perform on-site testing.

[0004] In order to meet the special needs of diagnosis and treatment in remote areas, emergency epidemic prevention and control, and other scenarios, there is an urgent need for technical means that can achieve rapid on-site detection of pathogens. Summary of the Invention

[0005] In view of the shortcomings of the existing technology, the present invention proposes a pathogen detection device and method to solve the technical problems in the existing technology that pathogen detection takes a long time and requires a special experimental environment.

[0006] The technical solution adopted by the present invention is a pathogen detection device and method.

[0007] The pathogen detection device includes a motor, a sample box, a liquid clearing mechanism and a filling mechanism;

[0008] The bottom of the sample box is detachably connected to the output shaft of the motor, and the liquid clearing mechanism and the filling mechanism are provided in the upper area of the sample box, for extracting substances from the sample box and adding substances to the sample box, respectively;

[0009] A round-bottomed accommodating cavity is provided inside the sample box, a ventilation channel is provided at the bottom of the accommodating cavity, and an elastic sealing film and a rubber plug are provided on the top. The rubber plug presses the edge of the elastic sealing film tightly against the mouth of the accommodating cavity. The sample box body is made of metal material, and an electromagnetic induction coil is provided on the outer periphery.

[0010] Optionally, the liquid clearing mechanism comprises a liquid clearing container, a liquid clearing device feeding mechanism and a liquid clearing feeding mechanism;

[0011] The head end of the clear liquid container is provided with a needle and faces the rubber plug, and the tail end is a piston structure including a first piston shell and a first piston rod. The clear liquid feed mechanism is sleeved on the first piston shell, and the clear liquid feed mechanism is sleeved on the first piston rod.

[0012] Optionally, the liquid clearing device feeding mechanism includes a first fixed ring and a first rotating ring, the first fixed ring is fixed to the housing of the pathogen detection device, the first rotating ring is rotatably matched with the first fixed ring, the inner ring surface of the first rotating ring is sleeved on the outer ring surface of the first piston shell, and they are threadedly matched with each other.

[0013] Optionally, the clear liquid feeding mechanism includes a second fixed ring and a second rotating ring, the second fixed ring is fixed to the housing of the pathogen detection device, the second rotating ring is rotatably matched with the second fixed ring, the inner ring surface of the second rotating ring is sleeved on the outer ring surface of the first piston rod, and they are threadedly matched with each other.

[0014] Optionally, the liquid clearing mechanism is provided at the center of the pathogen detection device, and the filling mechanism is provided at the periphery of the liquid clearing mechanism;

[0015] The filling mechanism includes a filling container, a filler feeding mechanism and a filling feeding mechanism;

[0016] The head end of the filling container is provided with a needle and faces the rubber plug, and the tail end is a piston structure including a second piston shell and a second piston rod. The filler feeding mechanism is sleeved on the second piston shell, and the filling feeding mechanism is sleeved on the second piston rod.

[0017] Optionally, the filling containers are at least two groups;

[0018] The filler feeding mechanism includes a third fixed ring and a third rotating ring. The third fixed ring is fixed to the housing of the pathogen detection device. The third rotating ring rotates with the third fixed ring. The number of the third rotating rings is consistent with the number of the filling containers and they are respectively sleeved on the outer ring surface of each second piston shell and are threadedly engaged with each other.

[0019] Optionally, the filling and feeding mechanism includes a fourth fixed ring and a fourth rotating ring, the fourth fixed ring is fixed to the housing of the pathogen detection device, the fourth rotating ring is rotatably matched with the fourth fixed ring, the inner ring surface of the fourth rotating ring is sleeved on the outer ring surface of the second piston rod, and they are threadedly matched with each other.

[0020] Optionally, the filling container and the clear liquid container are syringes, and the outer cylinder and the piston body of the syringe are fixedly sleeved with contact rings, and the contact rings are provided with external threads.

[0021] Optionally, a battery is further included, and the battery provides power to the motor and the electromagnetic induction coil.

[0022] The pathogen detection method, using the pathogen detection device described above, comprises the following steps:

[0023] The sample box is removed from the pathogen detection device, the elastic sealing membrane is sealed at the mouth of the accommodating cavity and the edge is pressed tightly with the rubber stopper, a syringe pierces the rubber stopper and injects the original specimen into the elastic sealing membrane until the elastic sealing membrane bulges and fills the accommodating cavity, and the syringe is removed;

[0024] The sample box is loaded into the pathogen detection device, the motor rotates and centrifuges the original specimen, the clearing mechanism pierces the rubber stopper and extracts the upper clear liquid from the sample box, and after completion, the clearing mechanism moves back;

[0025] The filling mechanism adds a first substance to the sample box and then moves back. After a first time period, the filling mechanism adds a second substance to the sample box and then moves back. An alternating current of a first power is applied to the electromagnetic induction coil, causing the sample box body to heat up. After a second time period, the electromagnetic induction coil is reduced to a second power. After a third time period, the filling mechanism adds a third substance to the sample box and then moves back. After a fourth time period, heating is stopped, and the filling mechanism adds a fourth substance to the sample box and then moves back.

[0026] It can be seen from the above technical solution that the beneficial technical effects of the present invention are as follows:

[0027] This method breaks through the traditional reliance on laboratory environments, enabling testing to be carried out smoothly in remote areas and outdoors where conditions are limited, greatly expanding the application scenarios of testing. At the same time, this testing method significantly shortens testing time, typically completing the test in less than an hour, significantly improving efficiency compared to traditional testing processes. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly describes the drawings required for the specific embodiments or the description of the prior art. Similar elements or parts are generally identified by similar reference numerals throughout the drawings. Elements or parts in the drawings are not necessarily drawn to scale.

[0029] Figure 1 It is an overall schematic diagram of the present invention;

[0030] Figure 2 This is a disassembled schematic diagram of the present invention;

[0031] Figure 3 This is a partial cutaway schematic diagram of the sample box area of the present invention;

[0032] Figure 4 Schematic diagram of the feed mechanism of the liquid clearing device and the feed mechanism of the filling device of the present invention;

[0033] Figure 5 This is a schematic diagram of the feed mechanism of the filler of the present invention;

[0034] Figure 6 This is a schematic diagram of a single injector of the present invention;

[0035] Figure 7 This is a schematic diagram of a fourth fixing ring of the present invention;

[0036] Figure 8 It is a half-section schematic diagram of the present invention;

[0037] Figure 9 For the present invention Figure 8 A partial enlarged schematic diagram in the middle;

[0038] Figure 10 A half-section schematic diagram of a sample box of the present invention;

[0039] Figure 11 It is a schematic cross-sectional view of the feeding mechanism of the present invention.

[0040] Wherein: motor 1, sample box 2, accommodating chamber 20, air permeable channel 21, sealing film 22, rubber stopper 23, electromagnetic induction coil 24, clear liquid container 30, first piston shell 301, first piston rod 302, clear liquid feed mechanism 31, first fixed ring 311, first rotating ring 312, clear liquid feed mechanism 32, second fixed ring 321, second rotating ring 322, filling container 40, second piston shell 401, second piston rod 402, filler feed mechanism 41, third fixed ring 411, third rotating ring 412, filling feed mechanism 42, fourth fixed ring 421, fourth rotating ring 422. DETAILED DESCRIPTION

[0041] The following embodiments of the technical solution of the present invention will be described in detail with reference to the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present invention and are therefore only examples and are not intended to limit the scope of protection of the present invention.

[0042] It should be noted that, unless otherwise specified, the technical or scientific terms used in this application should have the common meanings understood by those skilled in the art to which the present invention belongs.

[0043] This embodiment provides a pathogen detection device and method, one possible implementation of which is:

[0044] See Figure 1 and Figure 2The pathogen detection device is cylindrical in shape as a whole, and its overall appearance is like a large pen, including a motor 1, a sample box 2, a liquid clearing mechanism and a filling mechanism; the bottom of the sample box 2 can be detachably connected to the output shaft of the motor 1, and the specific connection method can be selected according to needs. For example, the simplest method can be a rectangular interface that is inserted into each other through friction, with one end protruding and the other end recessed, and an elastic rubber layer is provided on the connection interface to adapt to the cross-sectional deformation and friction requirements of insertion and extraction. The device can be used at any angle, but the best state of use is vertical. In this state, the liquid clearing mechanism and the filling mechanism are provided in the upper area of the sample box 2, which are used to extract substances from the sample box 2 and add substances to the sample box 2 respectively; a round-bottomed accommodating chamber 20 is provided inside the sample box 2, such as Figure 10 As shown, a ventilation channel 21 is provided at the bottom of the accommodating chamber 20, and an elastic sealing film 22 and a rubber plug 23 are provided at the top. The rubber plug 23 presses the edge of the elastic sealing film 22 against the opening of the accommodating chamber 20. The sample box 2 is made of metal material, and an electromagnetic induction coil 24 is provided on the periphery. The ventilation channel 21 can be provided on the protruding portion of the bottom of the sample box 2 connected to the motor shaft. To ensure that the ventilation channel 21 is connected to the external environment, a connecting ventilation hole can be provided in the recessed portion of the motor shaft end. To ensure balance, multiple ventilation holes can be distributed at equal intervals around the circumference.

[0045] The following is an introduction to the pathogen detection method based on this device:

[0046] First, the sample preparation and boxing operations are carried out. The sample box 2 is carefully disassembled and removed from the pathogen detection device. The elastic sealing film 22 is obtained and sealed at the mouth of the receiving cavity 20 and the edge is pressed tightly with the rubber plug 23. A closed space is formed between the elastic sealing film 22 and the rubber plug 23. The syringe pierces the rubber plug 23 and injects the original specimen into the elastic sealing film 22 until the elastic sealing film 22 bulges and fills the receiving cavity 20. Figure 10 When using the syringe, strictly follow the aseptic operation specifications, slowly pierce the stopper with the syringe needle without damaging the elastic sealing film 22, and then steadily and slowly inject the original specimen. During the injection process, closely observe the state of the elastic sealing film 22 until it bulges and completely fills the receiving cavity, reaching the state shown in the figure. Figure 10 The original specimen can be a sample extracted from a wound or other part, and the sample is placed in physiological saline (or other solutions, mainly to adapt to the survival environment of the target pathogen) for soaking to obtain the liquid.

[0047] The accommodating chamber 20 is set to a round bottom structure mainly due to the consideration of adapting to the expansion characteristics of the elastic sealing membrane 22. During the pathogen detection process, when the original specimen or subsequent substances are added to the accommodating chamber 20, the elastic sealing membrane 22 will expand due to changes in internal pressure. Compared with other shapes, the round bottom design can provide a more uniform and sufficient expansion space for the elastic sealing membrane 22. Its arc-shaped surface can better conform to the deformation of the elastic sealing membrane 22 in all directions, avoiding stress concentration of the elastic sealing membrane 22 during the expansion process due to sharp edges or irregular shapes, thereby ensuring that the elastic sealing membrane 22 can expand stably and smoothly and ensure sealing performance. A breathable channel 21 is set at the bottom of the accommodating chamber 20. Its key role is to maintain the pressure balance on both sides of the elastic sealing membrane 22. When the elastic sealing membrane 22 expands or contracts, the volume of gas in the accommodating chamber 20 will change accordingly. Without the vent channel 21, as the elastic sealing membrane 22 expands, the gas within the containment chamber 20 is compressed, increasing the pressure and potentially hindering further expansion of the elastic sealing membrane 22, or even causing it to rupture. Conversely, when the elastic sealing membrane 22 contracts, a negative pressure forms within the containment chamber 20, affecting the extraction of the substance and subsequent reactions. The presence of the vent channel 21 allows for gas exchange between the inside and outside of the containment chamber 20, maintaining pressure balance on both sides of the elastic sealing membrane 22. This ensures that the elastic sealing membrane 22 can expand and contract normally, ensuring the stable operation of the pathogen detection device and the reliability of the test results.

[0048] The elastic sealing membrane 22 can be made of a highly elastic, transparent rubber material. For example, ultra-thin rubber has excellent elasticity, can flexibly deform when subjected to external forces, and quickly return to its original shape after the external force is removed. Its transparency facilitates intuitive observation of the specimen's condition within the chamber, facilitating sample monitoring during pathogen detection. The elastic sealing membrane 22 can be cylindrical so that it does not cling to the bottom of the rubber stopper 23 when installed within the chamber 20, making it difficult for the syringe needle to penetrate the elastic sealing membrane 22. The elastic sealing membrane 22 is expandable to effectively accommodate the addition and removal of substances within the sealed state. During the pathogen detection process, different test substances, such as buffers, markers, and reaction reagents, need to be added to the sample box 2 multiple times, and the supernatant liquid is also extracted simultaneously. The expandable nature of the elastic sealing membrane 22 allows it to expand outward accordingly as the volume of the substance within the chamber 20 increases when substances are added, maintaining the sealed environment within the chamber 20 and preventing substance leakage and external contamination. When the clear liquid is extracted or the amount of the substance is reduced, the elastic sealing membrane 22 can shrink and always fit tightly above the substance in the accommodating cavity 20, thereby ensuring the stability and accuracy of the detection process.

[0049] The rubber plug 23 is made of a rubber material with excellent elasticity and self-healing properties. When the needle is inserted, the plug deforms; when the needle is withdrawn, its elastic recovery force causes the needle hole in the plug to quickly shrink and close. The material's inherent properties create a self-sealing seal, effectively preventing liquid leakage and external contamination. The device can be used normally even when inverted.

[0050] The sample box 2 is loaded into the pathogen detection device, and the output shaft of the motor 1 rotates and centrifuges the original specimen. The centrifugal operation is one of the key steps in the entire detection process. Through high-speed rotation, the density difference of different substances is used to separate the components in the original specimen into layers, where the upper layer is a relatively clear clear liquid, while the lower sediment is mainly cell debris, etc. After the centrifugation operation is completed, the clear liquid mechanism pierces the rubber plug 23 and extracts the upper clear liquid from the sample box 2. After completion, the clear liquid mechanism moves back. The upper clear liquid is mostly water, and the pathogen content is extremely low. The extraction can effectively remove excess water, so that subsequent detection can more accurately focus on pathogens such as cells and viruses retained in the sample box, thereby improving detection efficiency and accuracy;

[0051] After the concentration increases, the filling mechanism adds the first substance to the sample box 2 and then moves back. After a first time period, the filling mechanism adds the second substance to the sample box 2 and then moves back. An alternating current of a first power is passed through the electromagnetic induction coil 24. If a DC power supply is used, a DC / AC inverter device needs to be used in conjunction with it. The sample box 2 body heats up. After a second time period, the electromagnetic induction coil 24 is reduced to a second power. After a third time period, the filling mechanism adds the third substance to the sample box 2 and then moves back. After a fourth time period, heating is stopped, and the filling mechanism adds the fourth substance to the sample box 2 and then moves back.

[0052] In the above steps, the substances added, the interval time, and the temperature maintained are all different for different types of pathogens, and can be set specifically according to the characteristics of each pathogen. Taking the detection of Mycoplasma pneumoniae as an example, the main steps are as follows: adding a lysis solution (first substance) to the sample box to lyse the pathogenic cells. The lysis time is 5 to 10 minutes (first time period), aiming to release the pathogen-related components in the cells. Then, adding a PCR amplification reaction buffer (second substance) to the sample box, heating it to about 95 degrees (first power), can denature the DNA and prepare for subsequent amplification. After maintaining it for 1 to 2 minutes (second time period), reduce the heating power and wait (third time period) for the temperature to stabilize and maintain at 40 to 45 degrees. Add the amplification enzyme (third substance) and amplify it for about 45 minutes (fourth time period) to allow the target DNA fragment to replicate in large quantities. Then stop heating and inject the Mycoplasma pneumoniae detection solution (fourth substance). At this time, the sample box 2 can be removed and the results (such as color changes) can be observed through the detection plate.

[0053] The beneficial effects of the above-mentioned embodiment are significant. It breaks through the dependence of traditional testing on laboratory environments, enables testing to be carried out smoothly in remote areas and outdoors with limited conditions, and greatly expands the application scenarios of testing. At the same time, this detection method significantly shortens the detection time, and the detection can usually be completed within 1 hour. Compared with the traditional detection process, the efficiency is greatly improved. This efficient and convenient feature enables it to quickly respond to various emergency scenarios, such as sudden epidemics and field rescue, and provides strong support for timely obtaining test results and taking corresponding measures.

[0054] In this embodiment, the liquid clearing mechanism includes a liquid clearing container 30, a liquid clearing device feeding mechanism 31 and a liquid clearing feeding mechanism 32; Figure 11 、 Figure 3 and Figure 5 The head end of the clear liquid container 30 is provided with a needle and faces the rubber plug 23, and the tail end is a piston structure including a first piston shell 301 and a first piston rod 302. The clear liquid feed mechanism 31 is sleeved on the first piston shell 301, and the clear liquid feed mechanism 32 is sleeved on the first piston rod 302. The operation of the clear liquid feed mechanism 31 can allow the needle at the head end of the clear liquid container 30 to be inserted into or pulled out of the sample box 2. When inserted, the operation of the clear liquid feed mechanism 32 can suck the liquid inside the sample box 2 into the clear liquid container 30. Specifically, the upper layer of clear liquid in the clear liquid container 30 after centrifugation is sucked into the clear liquid container 30. The purpose is to suck out the liquid with a low pathogen content, increase the concentration of pathogen components inside the sample box 2, and facilitate the effectiveness of subsequent detection.

[0055] As a further solution of the above embodiment, this embodiment can refer to Figure 4 、 Figure 8 and Figure 9 The liquid clearing device feed mechanism 31 comprises a first fixed ring 311 and a first rotating ring 312. The first fixed ring 311 is fixed to the housing of the pathogen detection device, and the first rotating ring 312 rotates in conjunction with the first fixed ring 311. The inner ring surface of the first rotating ring 312 fits over the outer ring surface of the first piston housing 301, and the two rings engage with each other through a threaded connection. To facilitate rotation of the first rotating ring 312, a protruding strip is provided on its outer circumference to increase friction and enhance tactile feel. This allows the first rotating ring 312 to be rolled with the fingers, driving the axial movement of the first piston housing 301, thereby allowing the needle at the end of the first piston housing 301 to be inserted into or removed from the sample cartridge 2.

[0056] In this embodiment, Figure 4As shown, the clear liquid feed mechanism 32 is located at the rear end of the clear liquid feed mechanism 31. The clear liquid feed mechanism 32 includes a second fixed ring 321 and a second rotating ring 322. The second fixed ring 321 is fixed to the housing of the pathogen detection device. The second rotating ring 322 rotates in conjunction with the second fixed ring 321. The inner ring surface of the second rotating ring 322 is sleeved onto the outer ring surface of the first piston rod 302, and the two are engaged by a threaded connection. To facilitate the rotation of the second rotating ring 322, a protruding strip is provided on the outer circumference of the second rotating ring 322 to increase friction and feel. This makes it easier to roll the second rotating ring 322 with your fingers, driving the axial movement of the first piston rod 302, thereby allowing the needle at the end of the first piston housing 301 to insert into the sample cartridge 2 and draw the substance in the sample cartridge 2 into the first piston housing 301.

[0057] In the above embodiment, in order to move the clear liquid container 30 as a whole, the first rotating ring 312 and the second rotating ring 322 can be rolled synchronously, so that the first piston rod 302 and the first piston shell 301 move synchronously, so that the clear liquid container 30 can be inserted into or removed from the sample box 2. In order to ensure that the movement amount of the first piston rod 302 and the first piston shell 301 is consistent during synchronous rolling, the thread parameters of the first piston rod 302 and the first piston shell 301 are consistent (the pitch is the same); in order to absorb the upper layer of clear liquid from the sample box 2, only the second rotating ring 322 can be rolled to move the first piston shell 301 backward relative to the first piston shell 301.

[0058] This embodiment Figure 5 As shown, the liquid clearing mechanism is arranged at the center of the pathogen detection device, and the filling mechanism is arranged at the periphery of the liquid clearing mechanism; the filling mechanism includes a filling container 40, a filler feeding mechanism 41 and a filling feeding mechanism 42;

[0059] The filling container 40 has a needle at its head end facing the rubber plug 23 , and a piston structure including a second piston shell 401 and a second piston rod 402 at its tail end. The filler feeding mechanism 41 is sleeved on the second piston shell 401 , and the filling feeding mechanism 42 is sleeved on the second piston rod 402 .

[0060] There are at least two groups of filling containers 40, which can be designed according to the type of substances to be added. In order to ensure the versatility of the device, 4 to 5 groups can be designed to reasonably balance the number of filling containers 40 and the volume of each filling container 40;

[0061] The dispenser feed mechanism 41 comprises a third fixed ring 411 and a third rotating ring 412. The third fixed ring 411 is fixed to the pathogen detection device housing, while the third rotating ring 412 rotatably engages with the third fixed ring 411. The number of third rotating rings 412 matches the number of dispensers 40, and each third rotating ring 412 is threadedly engaged with the outer surface of each second piston housing 401. Rotating the third rotating ring 412 allows the dispenser 40, which is threadedly engaged with the third rotating ring 412, to move axially, allowing the needle tip to be inserted into or removed from the sample cartridge 2.

[0062] Furthermore, the filling and feeding mechanism 42 includes a fourth fixed ring 421 and a fourth rotating ring 422. The fourth fixed ring 421 is fixed to the housing of the pathogen detection device, and the fourth rotating ring 422 rotates in engagement with the fourth fixed ring 421. The inner ring surface of the fourth rotating ring 422 fits over the outer ring surface of the second piston rod 402, and the rings are threadedly engaged with each other. When the fourth rotating ring 422 is rotated, the second piston rod 402 of the filling container 40, which is threadedly engaged with the ring, moves axially, thereby pushing the substance in the filling container 40 into the sample cartridge 2.

[0063] In summary, in the above embodiment, the clearing process is to extract the supernatant after centrifugation from the sample box 2 to increase the concentration of pathogens, and the steps include:

[0064] The clear liquid container is in place: the first rotating ring 312 and the second rotating ring 322 are rolled synchronously. Since the thread parameters are consistent, the first piston rod 302 and the first piston shell 301 move axially synchronously, driving the needle at the head end of the clear liquid container 30 to insert into the sample box 2, preparing for subsequent clear liquid operations.

[0065] Extracting the supernatant: Only the second rotating ring 322 is rotated, and the first piston rod 302 moves backward relative to the first piston housing 301, generating negative pressure, sucking the supernatant in the sample box 2 after centrifugation into the supernatant container 30, sucking out the liquid with low pathogen content, and increasing the pathogen concentration.

[0066] Exit of the clear liquid container: The first and second rotating rings are synchronously rolled again to make the first piston rod 302 and the first piston shell 301 move in opposite directions synchronously, driving the needle at the head end of the clear liquid container 30 to be pulled out of the sample box 2, completing the clear liquid operation.

[0067] In the above embodiment, the filling process is to add reaction substances, such as amplification enzymes, color developing agents, etc., into the sample box 2 to promote rapid amplification of pathogens (or pathogenic nucleic acids) in the sample box 2 for easy detection. The steps include:

[0068] The filling container is in place: the third rotating ring 412 and the fourth rotating ring 422 corresponding to the filling container 40 are rotated synchronously. Due to the threaded fit, the piston shell and the piston body of the filling container 40 move axially synchronously, and the needle at the head end is inserted into the sample box 2 without contacting the liquid, creating conditions for filling the sample box 2 with substances.

[0069] Pushing and injecting substances: rotating the fourth rotating ring 422 corresponding to the filling container 40, through threaded engagement, the second piston rod 402 moves axially, pushing the substances in the filling container 40 into the sample box 2, completing the filling of the required substances.

[0070] Filling container exit: synchronously rotate the third rotating ring 412 and the fourth rotating ring 422 in opposite directions to move the filling container 40 axially in the opposite direction, driving the needle to be pulled out of the sample box 2, completing the group of filling operations. If there are other filling requirements, repeat the above steps.

[0071] The above embodiment design has significant beneficial effects. During the filling process, the elastic sealing film can always keep the sample box sealed to prevent external contamination. When clearing the liquid, the elastic sealing film can automatically reduce its volume as the liquid decreases to avoid wasting space; when filling, it can automatically expand its volume to adapt to the addition of substances. When full, the elastic sealing film fits tightly to the sample box 2, increasing the contact area and greatly improving the heat transfer efficiency. When the sample box 2 is hot, the elastic sealing film can be evenly heated and kept warm, creating a stable temperature environment for the internal reaction, ensuring that the detection process is carried out efficiently and accurately, and improving the overall detection performance.

[0072] In this embodiment, the refill container 40 and the clear liquid container 30 are syringes. Contact rings are fixedly mounted on the outer barrel and piston of the syringes, and the outer rings of these contact rings are externally threaded. Modifying conventional syringes to create the refill container 40 and the clear liquid container 30 offers significant advantages. The modified syringes, with their externally threaded contact rings, significantly enhance the device's applicability and material interchangeability.

[0073] In this embodiment, a rechargeable battery is also included to provide power to the motor 1 and the electromagnetic induction coil 24. This rechargeable design eliminates the need for frequent battery replacement, reduces costs, and facilitates use in remote areas or outdoors without a fixed power source. This improves the device's portability and practicality, facilitating efficient testing.

[0074] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some or all of the technical features therein. These modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and description of the present invention.

Claims

1. A pathogen detection device, characterized in that: It comprises a motor (1), a sample box (2), a liquid clearing mechanism and a filling mechanism; The bottom of the sample box (2) is connected to the output shaft of the motor (1); the liquid clearing mechanism and the filling mechanism are arranged in the upper area of the sample box (2), and are used for extracting substances from the sample box (2) and adding substances to the sample box (2), respectively; The sample box (2) is provided with a round-bottomed accommodating cavity (20) inside, a ventilation channel (21) is provided at the bottom of the accommodating cavity (20), and an elastic sealing film (22) and a rubber plug (23) are provided at the top, and the rubber plug (23) presses the edge of the elastic sealing film (22) tightly against the mouth of the accommodating cavity (20). The body of the sample box (2) is made of metal material, and an electromagnetic induction coil (24) is provided on the periphery.

2. The pathogen detection device according to claim 1, wherein: The liquid clearing mechanism comprises a liquid clearing container (30), a liquid clearing device feeding mechanism (31) and a liquid clearing feeding mechanism (32); The head end of the clear liquid container (30) is provided with a needle and faces the rubber plug (23), and the tail end is a piston structure including a first piston shell (301) and a first piston rod (302). The clear liquid feed mechanism (31) is sleeved on the first piston shell (301), and the clear liquid feed mechanism (32) is sleeved on the first piston rod (302).

3. The pathogen detection device according to claim 2, characterized in that The liquid clearing device feeding mechanism (31) comprises a first fixed ring (311) and a first rotating ring (312), wherein the first fixed ring (311) is fixed to the housing of the pathogen detection device, and the first rotating ring (312) is rotatably matched with the first fixed ring (311), and the inner ring surface of the first rotating ring (312) is sleeved on the outer ring surface of the first piston shell (301), and the two are threadedly matched with each other.

4. The pathogen detection device according to claim 3, characterized in that The clear liquid feeding mechanism (32) comprises a second fixed ring (321) and a second rotating ring (322), wherein the second fixed ring (321) is fixed to the housing of the pathogen detection device, and the second rotating ring (322) is rotatably engaged with the second fixed ring (321), and the inner ring surface of the second rotating ring (322) is sleeved on the outer ring surface of the first piston rod (302), and the two are engaged with each other through threads.

5. The pathogen detection device according to claim 2, wherein: The liquid clearing mechanism is arranged at the center of the pathogen detection device, and the filling mechanism is arranged at the periphery of the liquid clearing mechanism; The filling mechanism includes a filling container (40), a filler feeding mechanism (41) and a filling feeding mechanism (42); The filling container (40) is provided with a needle at the head end thereof and faces the rubber plug (23), and the tail end is a piston structure including a second piston shell (401) and a second piston rod (402). The filler feeding mechanism (41) is sleeved on the second piston shell (401), and the filling feeding mechanism (42) is sleeved on the second piston rod (402).

6. The pathogen detection device according to claim 5, wherein: The filling containers (40) are at least two groups; The filler feeding mechanism (41) includes a third fixed ring (411) and a third rotating ring (412). The third fixed ring (411) is fixed to the housing of the pathogen detection device, and the third rotating ring (412) is rotatably matched with the third fixed ring (411). The number of the third rotating rings (412) is consistent with the number of the filling containers (40) and is respectively sleeved on the outer ring surface of each second piston shell (401), and is threadedly matched with each other.

7. The pathogen detection device according to claim 5, wherein: The filling and feeding mechanism (42) comprises a fourth fixed ring (421) and a fourth rotating ring (422), wherein the fourth fixed ring (421) is fixed to the housing of the pathogen detection device, and the fourth rotating ring (422) is rotationally matched with the fourth fixed ring (421), and the inner ring surface of the fourth rotating ring (422) is sleeved on the outer ring surface of the second piston rod (402), and the rings are threadedly matched with each other.

8. The pathogen detection device according to claim 5, wherein: The filling container (40) and the clear liquid container (30) are syringes. The outer cylinder and the piston body of the syringe are fixedly sleeved with contact rings, and the contact rings are provided with external threads.

9. The pathogen detection device according to claim 1, wherein: It also includes a battery, which provides power to the motor (1) and the electromagnetic induction coil (24).

10. A pathogen detection method, characterized in that: Using the pathogen detection device as claimed in claim 1 comprises the following steps: The sample box (2) is disassembled from the pathogen detection device, the elastic sealing film (22) is sealed at the mouth of the accommodating cavity (20) and the edge is pressed tightly with the rubber plug (23), a syringe pierces the rubber plug (23) and injects the original specimen into the elastic sealing film (22) until the elastic sealing film (22) bulges and fills the accommodating cavity (20), and the syringe is removed; The sample box (2) is loaded into the pathogen detection device, the motor (1) rotates and centrifuges the original specimen, the clear liquid mechanism pierces the rubber plug (23) and extracts the upper clear liquid from the sample box (2), and after completion, the clear liquid mechanism moves back; The filling mechanism adds a first substance to the sample box (2) and then moves back. After a first time period, the filling mechanism adds a second substance to the sample box (2) and then moves back. An alternating current of a first power is passed through the electromagnetic induction coil (24), and the sample box (2) body is heated. After a second time period, the electromagnetic induction coil (24) is reduced to a second power. After a third time period, the filling mechanism adds a third substance to the sample box (2) and then moves back. After a fourth time period, heating is stopped, and the filling mechanism adds a fourth substance to the sample box (2) and then moves back.

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