Controllable fermentation culture system for nitrogen-fixing microorganisms

Abstract

The invention discloses a nitrogen-fixing microorganism controllable fermentation culture system, and relates to the technical field of microorganism fermention.The nitrogen-fixing microorganism controllable fermentation culture system comprises an outer box body, a first fermentation tank and a second fermentation tank, the first fermentation tank and the second fermentation tank are located in the upper position and the lower position in the outer box body respectively, an opening is formed in the surface of the left end of the outer box body, and the left end of the outer box body is connected with a built-in frame in a clamped mode through the opening; gas stripping mechanisms are arranged in the first fermentation tank and the second fermentation tank, telescopic mechanisms are arranged above the gas stripping mechanisms, a moving mechanism is arranged at the bottom of the built-in frame, and a fixing plate is connected to the surface of the front end of the built-in frame. By arranging a series of structures, the whole system can provide a plurality of closed fermentation environments, so that the temperature, the PH value and the oxygen concentration can be independently regulated and controlled corresponding to different fermentation stages of nitrogen-fixing microorganisms, meanwhile, a traditional mechanical structure for stirring a whole culture solution is abandoned, and the cost is reduced. The nitrogen-fixing microorganisms are prevented from being damaged by overlarge shearing force in the stirring process.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial fermentation, in particular to a controllable fermentation culture system for nitrogen-fixing microorganisms. Background Art

[0002] Nitrogen-fixing microorganisms are microorganisms that can convert atmospheric nitrogen into bioavailable ammonia or ammonium salts. This process, known as biological nitrogen fixation, is a key component of the natural nitrogen cycle and is crucial for maintaining nitrogen supplies for ecosystems and agricultural production.

[0003] Nitrogen-fixing microbial fermentation refers to the process of using microorganisms with the ability to biologically fix nitrogen, cultivating them through fermentation technology and applying them to agriculture, ecological restoration or industrial production, which can reduce dependence on chemical nitrogen fertilizers.

[0004] Currently, microbial fermentation is mainly carried out in fermentation tanks. However, when in use, traditional fermentation tanks provide a single enclosed space for microbial fermentation, which makes them unsuitable for microorganisms such as nitrogen-fixing microorganisms, which have different requirements for oxygen concentration, temperature and pH value in different fermentation stages. In addition, traditional fermentation tanks use a mechanical structure to stir the microorganisms, which causes the microorganisms to be damaged due to excessive shear force during the stirring process, affecting the fermentation process. Therefore, a controllable fermentation culture system for nitrogen-fixing microorganisms is proposed. Summary of the Invention

[0005] The object of the present invention is to provide a controllable fermentation culture system for nitrogen-fixing microorganisms to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a controllable fermentation culture system for nitrogen-fixing microorganisms, comprising an outer box body and a first fermentation tank and a second fermentation tank located at upper and lower positions inside the outer box body, respectively. The first fermentation tank and the second fermentation tank have the same shape and size. A display screen is installed on the front surface of the outer box body, a base is fixedly connected to the lower surface of the outer box body, and an inner cavity is opened inside the base. An opening is provided on the left end surface of the outer box body, and an internal frame is connected to the left end of the outer box body through the opening. The internal frame is a U-shaped plate structure, and a U-shaped pull rod is fixed on the left end surface of the internal frame, and the upper and lower ends of the U-shaped pull rod pass through. The built-in frame is connected to the first fermentation tank and the second fermentation tank respectively, and openings are provided on the upper and lower end surfaces of the first fermentation tank and the second fermentation tank, and the first fermentation tank and the second fermentation tank are connected by a connecting pipe. The first fermentation tank and the second fermentation tank are both provided with an air lift mechanism inside, and a telescopic mechanism is provided above the air lift mechanism. An addition port is provided at one end of the upper surface of the first fermentation tank and the second fermentation tank, a moving mechanism is provided at the bottom of the built-in frame, and a fixed plate is connected to the front end surface of the built-in frame, a fixing bolt is connected to the right end of the fixed plate, and a positioning hole is provided on the front end surface of the outer box body at the same horizontal height on the right side of the fixed plate.

[0007] Preferably, the first fermentation tank and the second fermentation tank are both provided with inner liners, and cavities are reserved between the first fermentation tank and the second fermentation tank and the inner liners. The left ends of the outer surfaces of the first fermentation tank and the second fermentation tank are connected to fixing rods, and the fixing rods are fixedly connected to the inner wall of the built-in frame.

[0008] Preferably, the gas lift mechanism includes an L-shaped tube, a vent pipe and an air head, the L-shaped tube is connected to the inner wall of the right end of the first fermentation tank and the second fermentation tank, the vent pipe is inserted into the interior of the L-shaped tube, and the top outer surface of the vent pipe is fixedly connected to the inner wall of the L-shaped tube, the top of the vent pipe is connected to the air head, and a plurality of through holes are evenly distributed on the outer surface of the air head.

[0009] Preferably, the end of the ventilation pipe away from the air head is flange-connected to a delivery pipe, and the delivery pipe is a telescopic hose structure. The end of the delivery pipe away from the ventilation pipe passes through the outer box and is connected to an air pump, and the air pump is fixedly connected to the rear end surface of the outer box.

[0010] Preferably, the telescopic mechanism includes a connecting rod, a connecting block, a guide rod, a screw rod and a motor. The connecting rod is connected to the top outer surface of the L-shaped tube, the top of the connecting rod is connected to the connecting block, the left and right ends of the connecting block are respectively interspersed with a guide rod and a screw rod, and the bottom end of the screw rod is connected to the motor.

[0011] Preferably, the outer surface of the connecting rod is sleeved with a sleeve, and the inner wall of the sleeve is connected with a rubber ring.

[0012] Preferably, the moving mechanism includes a base plate, a first roller and a second roller, the base plate is a broken line structure, the base plate is connected to the bottom left end of the built-in frame, a plurality of first rollers are installed at the bottom of the base plate, and a plurality of second rollers are installed on the lower surface of the built-in frame.

[0013] Preferably, a sliding groove is provided below the second roller and is opened on the inner wall of the bottom end of the outer box body.

[0014] Preferably, an insertion rod is provided below the slide groove, and the left end of the insertion rod is connected to the built-in frame, and the right end of the insertion rod passes through the interior of the base and is connected to a slider.

[0015] Preferably, transparent windows are installed on the front end surfaces of the first fermentation tank and the second fermentation tank, and the positions of the transparent windows correspond to the positions of the L-shaped tubes. Temperature sensors and pH detectors are provided on the outer surfaces of the first fermentation tank and the second fermentation tank, and the temperature sensors and pH detectors are electrically connected to the display screen.

[0016] Compared with the prior art, the present invention has the following beneficial effects: 1. This controllable fermentation culture system for nitrogen-fixing microorganisms has two closed fermentation environments in the entire system by setting up a first fermentation tank and a second fermentation tank. In this way, independent and effective regulation can be carried out according to the requirements of nitrogen-fixing microorganisms for temperature, pH value and oxygen concentration at different fermentation stages, thereby avoiding the difficulty in controlling environmental conditions caused by nitrogen-fixing microorganisms completing the entire fermentation stage in a single environment.

[0017] 2. This controllable fermentation culture system for nitrogen-fixing microorganisms is equipped with a gas stripping mechanism, which includes an L-shaped tube, a vent pipe and a gas head. The gas stripping mechanism introduces oxygen-containing air and inert gas into the first fermentation tank and the second fermentation tank, respectively, to meet the different fermentation requirements of nitrogen-fixing microorganisms. At the same time, it replaces the traditional mechanical structure to stir the entire culture solution, so that the nitrogen-fixing microorganisms will not be damaged by excessive shear force during the stirring process.

[0018] 3. This nitrogen-fixing microorganism controllable fermentation culture system is equipped with a telescopic mechanism, which includes a connecting rod, a connecting block, a guide rod, a screw and a motor. The height of the entire air lift mechanism can be adjusted to ensure that half of the top opening of the L-shaped tube is in the culture solution and the other half is above the culture solution, so that the water flow discharged from the inside of the L-shaped tube can push the entire liquid surface with sufficient power.

[0019] 4. This nitrogen-fixing microorganism controllable fermentation culture system is provided with a moving mechanism, which includes a bottom plate, a first roller and a second roller, so that the built-in frame can slide in the outer box, making it easy to change the positions of the first fermentation tank and the second fermentation tank according to the situation.

[0020] 5. This controllable fermentation culture system for nitrogen-fixing microorganisms, by setting up the above series of structures, enables the entire system to provide multiple closed fermentation environments, thereby corresponding to different fermentation stages of nitrogen-fixing microorganisms, facilitating independent regulation of temperature, pH value and oxygen concentration, and at the same time abandoning the traditional mechanical structure for stirring the entire culture solution, thereby preventing the nitrogen-fixing microorganisms from being damaged by excessive shear force during the stirring process. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the front cross-sectional structure of the present invention; Figure 2 This is a schematic diagram of the front view of the appearance structure of the present invention; Figure 3 Schematic diagram of the internal structure of the first fermentation tank and the second fermentation tank of the present invention; Figure 4 It is a schematic diagram of the gas mechanism structure of the present invention; Figure 5 This is a schematic diagram of the storage state of the present invention; Figure 6 This is a schematic diagram of the left side structure of the built-in frame of the present invention; Figure 7 It is a schematic diagram of the top view of the outer box body of the present invention.

[0022] In the figure: 1. Outer box; 2. Display screen; 3. Base; 4. Internal frame; 5. U-shaped pull rod; 6. First fermentation tank; 7. Second fermentation tank; 8. Inner liner; 9. Cavity; 10. Addition port; 11. L-shaped tube; 12. Sleeve; 13. Connecting rod; 14. Connecting block; 15. Guide rod; 16. Screw rod; 17. Motor; 18. Vent pipe; 19. Air head; 20. Fixing plate; 21. Positioning hole; 22. Bottom plate; 23. First roller; 24. Second roller; 25. Slide groove; 26. Insert rod; 27. Slider; 28. Connecting pipe; 29. Delivery pipe; 30. Air pump; 31. Transparent window; 32. Fixing rod. DETAILED DESCRIPTION

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0024] In the description of the present invention, it should be noted that the terms "upper," "lower," "inner," "outer," "front end," "rear end," "both ends," "one end," "the other end," and the like, indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely to facilitate and simplify the description of the present invention. They are not intended to indicate or imply that the devices or components referred to must have, be constructed, or operate in a specific orientation, and therefore should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0025] like Figure 1-7As shown, the controllable fermentation culture system of nitrogen-fixing microorganisms in this embodiment includes an outer box body 1 and a first fermentation tank 6 and a second fermentation tank 7 located at the upper and lower positions inside the outer box body 1, respectively. The first fermentation tank 6 and the second fermentation tank 7 have the same shape and size. The bottom end surfaces of the first fermentation tank 6 and the second fermentation tank 7 are both arc-shaped, so as to facilitate the discharge of materials in the tank body. A display screen 2 is installed on the front surface of the outer box body 1. The installation circuit between the display screen 2 and the outer box body 1 adopts conventional technical means, and two display screens 2 are provided. The two display screens 2 respectively display the internal temperature, pH value and other information of the first fermentation tank 6 and the second fermentation tank 7. The lower surface of the outer box body 1 is fixedly connected to a base 3, and an inner cavity is opened inside the base 3. 3 The outer surface is wrapped with a layer of shock-absorbing rubber pad. An opening is set on the left end surface of the outer box body 1, and the left end of the outer box body 1 is connected to the built-in frame 4 through the opening. By setting the opening, the entire left end surface of the outer box body 1 is exposed, so that the built-in frame 4 can slide in and out smoothly from the inside of the outer box body 1. The built-in frame 4 is a U-shaped plate structure. A U-shaped pull rod 5 is inserted and fixed on the left end surface of the built-in frame 4, and the upper and lower ends of the U-shaped pull rod 5 pass through the built-in frame 4 and are respectively connected to the first fermentation tank 6 and the second fermentation tank 7. The staff can control the built-in frame 4 to slide inside the outer box body 1 by pulling the U-shaped pull rod 5, thereby driving the first fermentation tank 6 and the second fermentation tank 7 to move out from the inside of the outer box body 1 for feeding. Operations such as, and during fermentation, it is located inside the outer box body 1, so as to be hidden and protected, the upper and lower end surfaces of the first fermentation tank 6 and the second fermentation tank 7 are provided with openings, and the first fermentation tank 6 and the second fermentation tank 7 are connected through a connecting pipe 28, the opening is provided at the center position of the surface of the first fermentation tank 6 and the second fermentation tank 7, and an electric control valve is provided, the electric control valve is connected to the switch installed on the front end surface of the outer box body 1 through a conventional circuit, and the two are connected through the connecting pipe 28, so that the fermented nitrogen-fixing microorganisms can convert the fermentation environment, and the first fermentation tank 6 and the second fermentation tank 7 can be disassembled and replaced between the connecting pipe 28, and the interior of the first fermentation tank 6 and the second fermentation tank 7 are provided with an air stripping mechanism, through The air stripping mechanism allows the culture solution inside the first fermentation tank 6 and the second fermentation tank 7 to circulate, replacing the traditional mechanical stirring method to avoid damage to nitrogen-fixing microorganisms due to excessive shear force. A telescopic mechanism is provided above the air stripping mechanism. The telescopic mechanism can change the internal height of the air stripping mechanism in the first fermentation tank 6 and the second fermentation tank 7 according to the liquid level of the nutrient solution, thereby ensuring that the air stripping mechanism can stably promote the circulation of the nutrient solution. An addition port 10 is provided at one end of the upper surface of the first fermentation tank 6 and the second fermentation tank 7. The nutrient solution and acid and alkali solution necessary for the fermentation process can be added to the interior of the first fermentation tank 6 and the second fermentation tank 7 through the addition port 10, and an electrically controlled valve is provided on the addition port 10.The electrically controlled valve is connected to a switch mounted on the front surface of the outer box body 1 via a conventional circuit. A movable mechanism is provided at the bottom of the internal frame 4, and a fixing plate 20 is connected to the front surface of the internal frame 4. This movable mechanism allows the internal frame 4 to slide stably relative to the outer box body 1. A fixing bolt is connected to the right end of the fixing plate 20, and a positioning hole 21 is provided on the front surface of the outer box body 1 at the same level as the right side of the fixing plate 20. When the internal frame 4 slides into the interior of the outer box body 1, the end of the fixing plate 20 will correspond to the position of the positioning hole 21. The fixing bolt can then be inserted to fix the fixing plate 20 to the outer box body 1, thereby securing the internal frame 4.

[0026] Specifically, the first fermentation tank 6 and the second fermentation tank 7 are each provided with an inner liner 8, and a cavity 9 is reserved between the first fermentation tank 6, the second fermentation tank 7 and the inner liner 8. The left ends of the outer surfaces of the first fermentation tank 6 and the second fermentation tank 7 are connected to a fixing rod 32, and the fixing rod 32 is fixedly connected to the inner wall of the built-in frame 4. The outer surfaces of the first fermentation tank 6 and the second fermentation tank 7 are both provided with a water inlet and a water outlet connected to the cavity 9. By injecting hot water into the net cavity 9, the internal temperature of the first fermentation tank 6 and the second fermentation tank 7 can be changed, and an electric heating wire can be built into the cavity 9 and a corresponding temperature controller can be configured to achieve electric heating of the water.

[0027] Furthermore, the air lift mechanism includes an L-shaped tube 11, a vent tube 18 and an air head 19. The L-shaped tube 11 is connected to the inner wall of the right end of the first fermentation tank 6 and the second fermentation tank 7. The vent tube 18 is inserted into the interior of the L-shaped tube 11, and the top outer surface of the vent tube 18 is fixedly connected to the inner wall of the L-shaped tube 11. The top of the vent tube 18 is connected to the air head 19, and the outer surface of the air head 19 is evenly distributed with a plurality of through holes. When the outside air is transported through the vent tube 18 and ejected through the air head 19, a large number of bubbles will be formed. After the bubbles are mixed with the culture liquid, the density of the gas is much lower than that of the liquid, so the density of the mixed flow is significantly lower than that of the pure liquid. Due to the reduction in density, the gas-liquid mixed flow is subjected to greater force in the gravitational field. The buoyancy of the mixture pushes the mixed flow upward, similar to the "bubble pump" effect. When the gas rises in the liquid, the surrounding liquid pressure gradually decreases, the bubble volume expands, and the buoyancy is further increased. During the rising process, the bubbles rub against the liquid, transferring the momentum of the gas to the liquid, forming a continuous flow. After the gas-liquid mixed flow reaches the high position, that is, the top of the L-shaped tube 11, the gas escapes and the liquid is discharged horizontally, thereby forming a water flow push, replacing the traditional mechanical stirring to stir the culture solution, avoiding damage to nitrogen-fixing microorganisms due to excessive shear force. The gas head 19 is provided with multiple through holes on its surface, so that the discharged gas can fully contact with the liquid. At the same time, the top part of the L-shaped tube 11 is a telescopic hose structure.

[0028] Furthermore, the flange of the end of the ventilation pipe 18 away from the air head 19 is connected to the delivery pipe 29, and the delivery pipe 29 is a telescopic hose structure. The end of the delivery pipe 29 away from the ventilation pipe 18 passes through the outer box body 1 and is connected to the air pump 30, and the air pump 30 is fixedly connected to the rear end surface of the outer box body 1. The air pump 30 can pump the air into the interior of the ventilation pipe 18 through the delivery pipe 29, and the telescopic hose structure of the delivery pipe 29 ensures that the built-in frame 4 will not be affected when sliding.

[0029] Furthermore, the telescopic mechanism includes a connecting rod 13, a connecting block 14, a guide rod 15, a screw rod 16 and a motor 17. The connecting rod 13 is connected to the top outer surface of the L-shaped tube 11, the top of the connecting rod 13 is connected to the connecting block 14, the left and right ends of the interior of the connecting block 14 are respectively interspersed with the guide rod 15 and the screw rod 16, and the bottom end of the screw rod 16 is connected to the motor 17. After the liquid level of the culture medium inside the first fermentation tank 6 and the second fermentation tank 7 is determined (considering factors such as the liquid level change during the fermentation process, it can be selected in the first fermentation tank 6 or the second fermentation tank 7 A liquid level sensor or other sensor is installed inside). At this time, by starting the motor 17, the screw rod 16 rotates, and the connecting block 14 slides up and down, and then the top height of the L-shaped tube 11 is changed through the connecting rod 13, so that the top opening ensures that half of it is in the culture liquid and the other half is above the culture liquid, so that the water flow discharged from the inside of the L-shaped tube 11 can push the entire liquid surface with sufficient power. The model of the motor 17 can be selected from existing products on the market according to actual needs, and its control switch is set on the front surface of the outer box body 1 through a conventional circuit.

[0030] Furthermore, the outer surface of the connecting rod 13 is sleeved with a sleeve 12, and the inner wall of the sleeve 12 is connected to a rubber ring. The sleeve 12 is connected through the first fermentation tank 6 and the second fermentation tank 7. The rubber ring arranged inside the sleeve tightly wraps the surface of the connecting rod 13 to avoid gaps that allow outside air to enter the interior of the first fermentation tank 6 and the second fermentation tank 7. At the same time, the friction generated by the rubber ring wrapping ensures that the connecting rod 13 is stable in the absence of external force and will not fall by itself due to gravity.

[0031] Furthermore, the moving mechanism includes a base plate 22, a first roller 23 and a second roller 24. The base plate 22 is a broken line structure. The base plate 22 is connected to the left end of the bottom of the built-in frame 4. A plurality of first rollers 23 are installed at the bottom of the base plate 22, and a plurality of second rollers 24 are installed on the lower surface of the built-in frame 4. Through the setting of the first roller 23 and the second roller 24, the sliding of the built-in frame 4 is smoother.

[0032] Furthermore, a slide groove 25 is provided below the second roller 24 and is opened on the inner wall of the bottom end of the outer box body 1. By providing the slide groove 25, the second roller 24 can be stuck in the inner wall of the outer box body 1 and move stably.

[0033] Furthermore, an insertion rod 26 is provided below the slide groove 25, and the left end of the insertion rod 26 is connected to the built-in frame 4, and the right end of the insertion rod 26 passes through the interior of the base 3 and is connected to a slider 27. By providing the insertion rod 26 and the slider 27, the built-in frame 4 as a whole will be limited when sliding, and there will be no sliding deviation or the whole will be completely separated from the outer box body 1.

[0034] Furthermore, a transparent window 31 is installed on the front end surface of the first fermentation tank 6 and the second fermentation tank 7, and the position of the transparent window 31 corresponds to the position of the L-shaped tube 11. The outer surfaces of the first fermentation tank 6 and the second fermentation tank 7 are provided with a temperature sensor and a pH detector, and the temperature sensor and the pH detector are electrically connected to the display screen 2. By providing the transparent window 31, it is convenient for the staff to intersperse the liquid level conditions inside the first fermentation tank 6 and the second fermentation tank 7, so as to accurately adjust the position height of the L-shaped tube 11. The models of the temperature sensor and the pH detector are selected from existing products on the market according to actual needs, and the detection ends of the two pass through the interior of the first fermentation tank 6 and the second fermentation tank 7.

[0035] The method of use of this embodiment is as follows: first, the staff pulls the U-shaped pull rod 5 to pull the internal frame 4 out from the interior of the outer box 1, so that the first fermentation tank 6 and the second fermentation tank 7 are exposed to the outside world. At this time, the valve at the top opening of the first fermentation tank 6 is opened, and the nitrogen-fixing microorganisms are loaded into the interior of the first fermentation tank 6 and then the corresponding valves are closed. At this time, the liquid level inside the first fermentation tank 6 is penetrated through the transparent window 31, and then the motor 17 connected to the first fermentation tank 6 is started to control the rotation of the screw rod 16, thereby pushing the connecting block 14 to slide up and down, so as to control the top of the L-shaped tube 11 to move up and down through the connecting rod 13, ensuring that half of its top opening is in the culture solution and the other half is above the culture solution, so that the water flow discharged from the inside of the L-shaped tube 11 can push the entire liquid level with sufficient power. After the adjustment is completed, the internal frame 4 is pushed back into the interior of the outer box 1 for fermentation of nitrogen-fixing microorganisms. At the initial stage of fermentation, that is, after the nitrogenase activity protection stage, the bottom valve of the first fermentation tank 6 is opened to allow the nitrogen-fixing microorganism culture liquid to flow into the second fermentation tank 7 as a whole, and the nitrogen fixation period, that is, the bacterial growth and nutrient absorption stage, is carried out. When the nitrogenase is fermented at different stages inside the first fermentation tank 6 and the second fermentation tank 7, the staff respectively regulates the pH value and temperature of the fermentation environment. At the same time, the air stripping mechanism is used to pass oxygen-containing air in the first fermentation tank 6, and inert gas is introduced into the second fermentation tank 7 to ensure that the nitrogen-fixing microorganisms accurately control the oxygen, temperature and pH value at different fermentation stages. At the same time, the air stripping mechanism is used for air stripping and transportation, so that the nutrient solution can be pushed to circulate, replacing the traditional mechanical structure to stir the entire nutrient solution, thereby avoiding damage to the nitrogen-fixing microorganisms. After the entire fermentation process is completed, the nitrogen-fixing microorganisms can be discharged and collected as a whole by opening the bottom valve of the second fermentation tank 7.

[0036] Finally, it should be noted that the above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or substitute equivalents for some of the technical features. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A controllable fermentation culture system for nitrogen-fixing microorganisms, comprising an outer box (1) and a first fermentation tank (6) and a second fermentation tank (7) located at upper and lower positions inside the outer box (1), respectively, wherein the first fermentation tank (6) and the second fermentation tank (7) have the same shape and size, and are characterized by: The front end surface of the outer box (1) is provided with a display screen (2), the lower surface of the outer box (1) is fixedly connected with a base (3), and an inner cavity is provided inside the base (3), the left end surface of the outer box (1) is provided with an opening, and the left end of the outer box (1) is connected with an internal frame (4) through the opening, and the internal frame (4) is in a U-shaped plate structure, and a U-shaped pull rod (5) is fixedly inserted into the left end surface of the internal frame (4), and the upper and lower ends of the U-shaped pull rod (5) pass through the internal frame (4) and are respectively connected to the first fermentation tank (6) and the second fermentation tank (7), and the upper and lower end surfaces of the first fermentation tank (6) and the second fermentation tank (7) are provided with The first fermentation tank (6) and the second fermentation tank (7) are connected by a connecting pipe (28), the first fermentation tank (6) and the second fermentation tank (7) are both provided with an air lift mechanism, a telescopic mechanism is provided above the air lift mechanism, an addition port (10) is provided at one end of the upper surface of the first fermentation tank (6) and the second fermentation tank (7), a moving mechanism is provided at the bottom of the built-in frame (4), and a fixing plate (20) is connected to the front end surface of the built-in frame (4), a fixing bolt is connected to the right end of the fixing plate (20), and a positioning hole (21) is provided on the front end surface of the outer box body (1) at the same level as the right side of the fixing plate (20).

2. The controlled fermentation culture system for nitrogen-fixing microorganisms according to claim 1, characterized in that: The first fermentation tank (6) and the second fermentation tank (7) are both provided with an inner liner (8), and a cavity (9) is reserved between the first fermentation tank (6) and the second fermentation tank (7) and the inner liner (8). The left ends of the outer surfaces of the first fermentation tank (6) and the second fermentation tank (7) are both connected to a fixing rod (32), and the fixing rod (32) is fixedly connected to the inner wall of the built-in frame (4).

3. The controlled fermentation culture system for nitrogen-fixing microorganisms according to claim 1, characterized in that: The gas lift mechanism includes an L-shaped tube (11), a vent tube (18) and a gas head (19), wherein the L-shaped tube (11) is connected to the inner wall of the right end of the first fermentation tank (6) and the second fermentation tank (7), the vent tube (18) is inserted into the interior of the L-shaped tube (11), and the top outer surface of the vent tube (18) is fixedly connected to the inner wall of the L-shaped tube (11), the top end of the vent tube (18) is connected to the gas head (19), and the outer surface of the gas head (19) is evenly distributed with a plurality of through holes.

4. A controlled fermentation culture system for nitrogen-fixing microorganisms according to claim 3, characterized in that: The end of the vent pipe (18) away from the air head (19) is flange-connected to a delivery pipe (29), and the delivery pipe (29) is a telescopic hose structure. The end of the delivery pipe (29) away from the vent pipe (18) passes through the outer box (1) and is connected to an air pump (30), and the air pump (30) is fixedly connected to the rear end surface of the outer box (1).

5. The controlled fermentation culture system for nitrogen-fixing microorganisms according to claim 1, characterized in that: The telescopic mechanism comprises a connecting rod (13), a connecting block (14), a guide rod (15), a screw rod (16) and a motor (17). The connecting rod (13) is connected to the top outer surface of the L-shaped tube (11). The top of the connecting rod (13) is connected to the connecting block (14). The left and right ends of the connecting block (14) are respectively inserted with the guide rod (15) and the screw rod (16). The bottom end of the screw rod (16) is connected to the motor (17).

6. The controlled fermentation culture system for nitrogen-fixing microorganisms according to claim 5, characterized in that: The outer surface of the connecting rod (13) is sleeved with a sleeve (12), and the inner wall of the sleeve (12) is connected to a rubber ring.

7. The controlled fermentation culture system for nitrogen-fixing microorganisms according to claim 1, characterized in that: The moving mechanism comprises a bottom plate (22), a first roller (23) and a second roller (24); the bottom plate (22) is in a broken line structure; the bottom plate (22) is connected to the left end of the bottom of the built-in frame (4); a plurality of first rollers (23) are mounted on the bottom of the bottom plate (22); and a plurality of second rollers (24) are mounted on the lower surface of the built-in frame (4).

8. The controlled fermentation culture system for nitrogen-fixing microorganisms according to claim 7, characterized in that: A sliding groove (25) is provided below the second roller (24) and is opened on the inner wall of the bottom end of the outer box body (1).

9. The controlled fermentation culture system for nitrogen-fixing microorganisms according to claim 8, characterized in that: An insertion rod (26) is provided below the slide groove (25), and the left end of the insertion rod (26) is connected to the built-in frame (4), and the right end of the insertion rod (26) passes through the interior of the base (3) and is connected to a slider (27).

10. The controlled fermentation culture system for nitrogen-fixing microorganisms according to claim 1, characterized in that: A transparent window (31) is installed on the front end surface of each of the first fermentation tank (6) and the second fermentation tank (7), and the position of the transparent window (31) corresponds to the position of the L-shaped tube (11). A temperature sensor and a pH detector are provided on the outer surface of each of the first fermentation tank (6) and the second fermentation tank (7), and the temperature sensor and the pH detector are electrically connected to the display screen (2).

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