Filter type kinase integrated reaction device

Abstract

The invention discloses a filtering type kinase integrated reaction device, and relates to the technical field of kinase reaction, the filtering type kinase integrated reaction device comprises a device main body and a reaction box body, the device main body comprises a stirring assembly, a transmission assembly connected with the stirring assembly, and a conveying assembly connected with the transmission assembly, the conveying assembly is connected with the conveying frame, the filtering assembly is connected with the conveying assembly, the cleaning assembly is connected with the filtering assembly, the conveying assembly comprises a second motor and a first belt wheel, and a rotating shaft B is arranged on the second motor. A motor II drives a conveying belt and an extrusion sheet to rotate, and the kinase enters the stirring box body again, is effectively stirred and filtered for multiple times through stirring blades, and finally enters a shaking frame I and a shaking frame II to be filtered for multiple times, so that the kinase is effectively treated for multiple times.

Description

Technical Field

[0001] The present invention relates to the technical field of kinase reactions, in particular to an integrated reaction device for filtering kinases. Background Art

[0002] Immobilized enzyme technology simulates the action of enzymes in vivo. Through chemical or physical means, enzymes are bound or confined to a specific area with a carrier, allowing the enzyme molecules to perform unique and active catalytic actions in this area. The technology can also be recovered and reused multiple times. Currently, the theoretical and applied research of immobilized enzymes has achieved many important results and has been widely used in the fields of food, medicine, chemical industry, etc. In the field of kinase reaction production, traditional treatment methods have significant disadvantages. On the one hand, the existing filtration device has a single function and cannot effectively treat kinase multiple times. During filtration, kinase is prone to local accumulation and blockage of the filter material pores, resulting in a significant increase in filtration resistance and reduced efficiency. On the other hand, kinase is not sufficiently dispersed in the reaction system and the materials are unevenly mixed, which seriously affects the reaction and is difficult to meet the current high-quality and high-efficiency production requirements. For this reason, we propose an integrated reaction device for filtering kinase. Summary of the Invention

[0003] The object of the present invention is to provide an integrated reaction device for filtering kinase.

[0004] To achieve the above-mentioned object, the present invention provides the following technical solution: an integrated reaction device for filtering kinase, comprising a device body and a reaction box, the device body comprising a stirring assembly, a transmission assembly connected to the stirring assembly, a conveying assembly connected to the transmission assembly, a filtering assembly connected to the conveying assembly, and a cleaning assembly connected to the filtering assembly, the conveying assembly comprising a second motor and a first pulley, the second motor being provided with a rotating shaft B, the first pulley being provided on the rotating shaft B, the first pulley and the second pulley being provided with a transmission belt, the other end of the rotating shaft B being provided with a conveyor belt, the conveyor belt being provided with a plurality of extrusion sheets, the plurality of extrusion sheets being provided with a soft section on one side close to the conveyor belt, the outer sides of the conveyor belt and the plurality of extrusion sheets being provided with a conveying frame, the filtering assembly comprising a second pulley, the second pulley being provided with a rotating shaft C, the rotating shaft C being provided with a linkage rod, the linkage rod rotating around the rotating shaft C, the cleaning assembly comprising a fixed frame, the fixed frame being provided with a slide groove, the interior of the slide groove being slidably connected to a slider, the front bottom surfaces of the sliders being respectively connected to a shaking frame 1 and a shaking frame 2.

[0005] As a further solution of the present invention: the stirring assembly includes motor 1, bevel gear 1, bevel gear 2 and stirring blades, the motor 1 is provided with a rotating shaft A, the bevel gear 1 is provided on the rotating shaft A, the bevel gear 1 and the bevel gear 2 are meshed, the bevel gear 2 is provided with a stirring shaft, and multiple stirring blades are evenly arranged on the stirring shaft.

[0006] As a further solution of the present invention: a protective box is provided on the outer sides of the bevel gear 1 and the bevel gear 2, and a position sensor is provided on the slider.

[0007] As a further solution of the present invention: a fixing groove is provided at the bottom of the fixing frame, a telescopic rod is provided inside the fixing groove, a cleaning brush 1 and a cleaning brush 2 are provided in front of the fixing frame, the rotating shaft C passes through the interior of the fixing frame, and the fixing frame is provided on the inner wall of the reaction box.

[0008] As a further solution of the present invention: the shaking frame 1 and the shaking frame 2 are both provided with filter screens, and the cleaning brush 1 and the cleaning brush 2 are respectively attached to the shaking frame 1 and the shaking frame 2.

[0009] As a further solution of the present invention: the lengths of the cleaning brush 1 and the cleaning brush 2 correspond to the diameters of the shaking frame 1 and the shaking frame 2 respectively.

[0010] As a further solution of the present invention: a feeding box and a stirring box are respectively provided inside the reaction box from top to bottom, and an outlet is opened on the bottom surface of the stirring box.

[0011] As a further solution of the present invention: the transmission assembly is arranged on bevel gear 1, the transmission assembly includes a transmission auger and a barrel-shaped net, the transmission auger is arranged inside the barrel-shaped net, the bottom of the conveying frame is connected to the barrel-shaped net, the right side of the conveying frame is connected to the stirring box, an inlet is opened on the barrel-shaped net, the inlet and outlet correspond to each other, and a biosensor is provided on the inner wall of the stirring box.

[0012] As a further solution of the present invention: a controller is provided in front of the device body, and the controller is electrically connected to the motor 1, the motor 2 and the position sensor respectively.

[0013] By adopting the above technical solution, compared with the prior art, the beneficial effects of the present invention are: 1. The present invention drives the transmission auger through the motor 1, and transmits the kinase to the inside of the conveying frame. The motor 2 drives the conveyor belt and the extrusion sheet to rotate. The kinase re-enters the interior of the mixing box and is effectively stirred and filtered multiple times by the stirring blades. Finally, it enters the interior of the shaking frame 1 and the shaking frame 2 for multiple filtrations, thereby achieving multiple and effective kinase treatments and uniform material mixing, which meets the current high-quality and high-efficiency production requirements. 2. The present invention starts the second motor to rotate, driving the first pulley and the transmission belt, which in turn drives the second pulley to rotate, driving the linkage rod to slide inside the chute, thereby driving the first and second shaking frames to shake fully, fully breaking up the kinase compound and promoting the reaction; 3. The present invention arranges a cleaning brush 1 and a cleaning brush 2 which are respectively attached to the shaking frame 1 and the shaking frame 2. The telescopic rod is started to drive the cleaning brush 1 and the cleaning brush 2 to clean the filter screen, so as to facilitate the cleaning of the filter screen after filtration for subsequent operations without clogging the pores of the filter material.

[0014] Other advantages, objects and features of the present invention will be described in part in the following description and, in part, will be apparent to those skilled in the art based on an examination of the following or may be learned from the practice of the invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a first stereoscopic schematic diagram of an embodiment of the present invention; Figure 2 This is a second stereoscopic schematic diagram of an embodiment of the present invention; Figure 3 Schematic diagram of the transport assembly, entrance, shaking frame 1 and shaking frame 2 in an embodiment of the present invention; Figure 4 This is a schematic diagram of the connection structure of the protection box, the stirring assembly, and the transmission assembly in an embodiment of the present invention; Figure 5 Schematic diagram of the connection structure of the stirring assembly, the protective box, the extrusion assembly and the shaking assembly in an embodiment of the present invention; Figure 6 Schematic diagram of the connection structure between the transport assembly and the transport frame in an embodiment of the present invention; Figure 7 Schematic diagram of the connection structure of the position sensor and the filter assembly in an embodiment of the present invention; Figure 8 Schematic diagram of a cleaning component in an embodiment of the present invention.

[0016] In the figure: 1. Device body; 2. Reaction box; 3. Stirring box; 4. Feeding box; 5. Stirring assembly; 51. Motor 1; 52. Rotating shaft A; 53. Bevel gear 1; 54. Bevel gear 2; 55. Stirring blade; 6. Protective box; 7. Transport assembly; 71. Motor 2; 72. Pulley 1; 73. Rotating shaft B; 74. Transmission belt; 75. Conveyor belt; 76. Extrusion sheet; 761. Soft section; 8. Conveyor frame; 9. Transmission Dynamic component; 91. Transmission auger; 92. Barrel net; 10. Position sensor; 11. Filter component; 111. Pulley 2; 112. Rotating shaft C; 113. Linkage rod; 114. Slide; 115. Swing frame 1; 116. Swing frame 2; 12. Cleaning component; 121. Fixed frame; 122. Telescopic rod; 123. Cleaning brush 1; 124. Cleaning brush 2; 13. Exit; 14. Entrance; 15. Biosensor. DETAILED DESCRIPTION

[0017] The specific embodiments of the present invention will be further described below in conjunction with the accompanying drawings. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention.

[0018] In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0019] In the field of modern biopharmaceuticals and biochemical research, kinases, as an important type of biocatalyst, play a key role in numerous biological reaction processes. Reactions involving kinases usually require precise control of reaction conditions and efficient separation and purification steps to ensure the acquisition of target products with high purity and high activity. Currently, kinase reactions and filtration separation processes mostly use relatively independent equipment and processes. Traditional kinase reaction devices often focus on the control of reaction conditions, such as temperature, pH value, and reactant concentration, but have many defects in the filtration and separation link after the reaction. Existing filtration devices generally have a single function and an unreasonable structural design, and cannot be flexibly adjusted according to the actual situation of kinase filtration. Therefore, the present invention provides an integrated reaction device for filtration-type kinase, please refer to the attached Figure 1 -Attached Figure 8, including a device body 1 and a reaction box body 2, the device body 1 includes a stirring component 5, a transmission component 9 connected to the stirring component 5, a conveying component 7 connected to the transmission component 9, a filtering component 11 connected to the conveying component 7 and a cleaning component 12 connected to the filtering component 11, the conveying component 7 includes a second motor 71 and a pulley 72, a rotating shaft B73 is provided on the second motor 71, a pulley 72 is provided on the rotating shaft B73, a transmission belt 74 is provided on the pulley 72 and the pulley 111, a conveyor belt 75 is provided at the other end of the rotating shaft B73, a plurality of extrusion sheets 76 are provided on the conveyor belt 75, and a soft section 761 is provided on the side of the plurality of extrusion sheets 76 close to the conveyor belt 75, a conveying frame 8 is provided on the outer side of the conveyor belt 75 and the plurality of extrusion sheets 76, the filtering component 11 includes a second pulley 1 11. A rotating shaft C112 is provided on the pulley 2 111, and a linkage rod 113 is provided on the rotating shaft C112. The linkage rod 113 rotates around the rotating shaft C112. The cleaning component 12 includes a fixed frame 121. A slide groove 114 is provided on the fixed frame 121. The internal sliding connection of the slide groove 114 is connected with a slider. The bottom surface of the front of the slider is respectively connected with a shaking frame 115 and a shaking frame 2 116. The kinase enters the interior of the transmission component 9 through the kinase, and the kinase is fully squeezed by the multiple squeezing sheets 76 in the conveying component 7, and finally re-enters the interior of the mixing box 3, so as to fully squeeze the kinase inside the mixing box 3, which can effectively promote the uniform distribution of the kinase in the mixing box 3, and make up for the insufficient reaction problem caused by local accumulation or uneven distribution of the kinase, thereby achieving effective supplementation and optimization of the kinase reaction process.

[0020] Embodiment 2, the stirring assembly 5 includes a motor 1 51, a bevel gear 1 53, a bevel gear 2 54 and a stirring blade 55, a rotating shaft A52 is provided on the motor 1 51, the bevel gear 1 53 is provided on the rotating shaft A52, the bevel gear 1 53 and the bevel gear 2 54 are meshed, a stirring shaft is provided on the bevel gear 2 54, a plurality of stirring blades 55 are evenly arranged on the stirring shaft, a protective box 6 is provided on the outside of the bevel gear 1 53 and the bevel gear 2 54, a position sensor 10 is provided on the slider, and two holes are provided on the right side of the conveying frame 8, one of the holes is connected to the barrel net 92, and the other hole is connected to the stirring box 3; Specifically, the motor 1 51 drives the bevel gear 2 54 to rotate and drive the multiple stirring blades 55 on the stirring shaft to stir the kinase. At the same time, the kinase enters the interior of the barrel-shaped net 92 through the inlet 14, is transported by the transmission auger 91, and finally the motor 2 71 drives the pulley 1 72 to rotate, and the multiple extrusion plates 76 drive the kinase to re-enter the interior of the stirring box 3, thereby achieving multiple effective treatments of the kinase. Finally, the kinase enters the interior of the reaction box 2 for subsequent operations. Specifically, the soft section 761 is provided to facilitate the transport of the kinase back to the interior of the mixing box 3 , and the distance between the two extrusion sheets 76 is consistent with the distance between the holes opened on the conveying frame 8 .

[0021] Example 3: A fixing groove is provided at the bottom of the fixed frame 121, a telescopic rod 122 is provided inside the fixing groove, a cleaning brush 123 and a cleaning brush 2 124 are provided in front of the fixed frame 121, the rotating shaft C112 passes through the interior of the fixed frame 121, the fixed frame 121 is arranged on the inner wall of the reaction box 2, and a filter is provided on the shaking frame 1 115 and the shaking frame 2 116. The cleaning brush 1 123 and the cleaning brush 2 124 are respectively attached to the shaking frame 1 115 and the shaking frame 2 116, and the lengths of the cleaning brush 1 123 and the cleaning brush 2 124 correspond to the diameters of the shaking frame 1 115 and the shaking frame 2 116, respectively. Specifically, the telescopic rod 122 is provided to drive the cleaning brush 1 123 and the cleaning brush 2 124 to extend and retract, so as to facilitate the cleaning of the shaking frame 1 115 and the shaking frame 2 116, thereby preventing the problem of clogging of the two filter screens.

[0022] Example 4. The interior of the reaction box 2 is respectively provided with a feeding box 4 and a stirring box 3 from top to bottom. The bottom surface of the stirring box 3 is provided with an outlet 13, and the transmission assembly 9 is arranged on the bevel gear 53. The transmission assembly 9 includes a transmission auger 91 and a barrel-shaped net 92. The transmission auger 91 is arranged inside the barrel-shaped net 92. The bottom of the conveying frame 8 is connected to the barrel-shaped net 92, and the right side of the conveying frame 8 is connected to the stirring box 3. An inlet 14 is provided on the barrel-shaped net 92, and the inlet 14 and the outlet 13 correspond to each other. A biosensor 15 is provided on the inner wall of the stirring box 3, which can monitor the pH value and temperature of the reaction system in real time and detect the uniformity of material mixing. When it is detected that specific impurities exist in the filtered material or the target kinase concentration does not meet the expectation, a feedback signal is given to the controller to adjust and optimize the filtration process; Specifically, by setting a position sensor 10, when the kinase enters the interior of the stirring box 3, the starting motor 2 71 drives the linkage rod 113 to transmit on the rotating shaft C112, and then drives the shaking frame 1 115 and the shaking frame 2 116 to shake, so that the kinase is fully dispersed and processed in the reaction system.

[0023] Embodiment 5: A controller is provided on the front of the device body 1, and the controller is electrically connected to the motor 1 51, the motor 2 71 and the position sensor 10 respectively; Specifically, the controller uses PID control. When the kinase compound needs to be dispersed, the position sensor 10 senses the kinase compound and starts the second motor 71 to rotate, driving the first pulley 72 and the transmission belt 74 to drive. In turn, the second pulley 111 rotates, driving the linkage rod 113 to slide inside the slide 114, thereby driving the shaking frame 115 and the shaking frame 116 to shake fully, fully breaking up the kinase compound and promoting the reaction. Specifically, the transmission screw 91 is driven by the motor 1 51 to transmit the kinase to the interior of the conveying frame 8, the motor 2 71 drives the conveyor belt 75 and the extrusion sheet 76 to rotate, and the kinase re-enters the interior of the mixing box 3 and is effectively stirred and filtered multiple times by the stirring blade 55, and finally enters the interior of the shaking frame 1 115 and the shaking frame 2 116 for multiple filtration, thereby achieving multiple effective kinase treatments and uniform material mixing, which meets the current high-quality and high-efficiency production requirements; The telescopic rod 122 in the cleaning component 12 drives the cleaning brush 1 123 and the cleaning brush 2 124 to effectively clean the shaking frame 1 115 and the shaking frame 2 116 respectively, avoiding the problem of local blockage of kinase and accumulation during multiple filtrations.

[0024] Working principle: First, move the device body 1 to the desired position, and place the kinase to be processed into the inside of the feed box 4. The kinase first enters the inside of the stirring box 3. At this time, start the motor 1 51 to drive the rotating shaft A52, the bevel gear 1 53 and the bevel gear 2 54 to rotate, thereby driving the stirring blade 55 to stir the kinase inside the stirring box 3. The biosensor 15 detects the mixing degree of the kinase after stirring in real time. The kinase falls from the outlet 13 to the position of the inlet 14 and enters the inside of the barrel-shaped net 92. The barrel-shaped net 92 filters the kinase for the first time. After the transmission of the transmission auger 91, the kinase enters the inside of the conveying frame 8. At this time, turn on the motor 2 71, and the motor 2 71 drives the conveyor belt 75 and the conveyor The extrusion piece 76 on the conveyor belt 75 squeezes the kinase, and the kinase re-enters the interior of the mixing box 3. The kinase falls from the inside of the barrel-shaped net 92 to the inside of the shaking frame 115 and the shaking frame 2 116. The position sensor 10 senses it, and the transmission belt 74 drives the pulley 1 72 and the pulley 2 111 to rotate, driving the linkage rod 113 to slide inside the slide groove 114, thereby driving the shaking frame 115 and the shaking frame 2 116 to shake fully, realizing multiple effective treatments of the kinase. After use, start the telescopic rod 122 to drive the cleaning brush 123 and the cleaning brush 2 124 to clean the shaking frame 115 and the shaking frame 2 116. At this point, the entire work flow is completed.

[0025] The above-mentioned front, back, left, right, up and down are all based on the Figure 1 As a benchmark.

[0026] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the scope of protection of the present invention.

[0027] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings, but the present invention is not limited to the described embodiments.

[0028] It is obvious to those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and these changes still fall within the scope of protection of the present invention.

Claims

1. An integrated reaction device for filtering kinase, comprising a device body (1) and a reaction chamber (2), characterized in that: The device body (1) includes a stirring assembly (5), a transmission assembly (9) connected to the stirring assembly (5), a conveying assembly (7) connected to the transmission assembly (9), a filtering assembly (11) connected to the conveying assembly (7), and a cleaning assembly (12) connected to the filtering assembly (11). The conveying assembly (7) includes a second motor (71) and a first pulley (72). A rotating shaft B (73) is provided on the second motor (71). The first pulley (72) is provided on the rotating shaft B (73). A transmission belt (74) is provided on the first pulley (72) and the second pulley (111). A conveying belt (75) is provided at the other end of the rotating shaft B (73). A plurality of extrusion sheets (76) are provided on the conveying belt (75). A soft section (761) is provided on one side of the extrusion sheet (76) close to the conveyor belt (75), and a conveying frame (8) is provided on the outer side of the conveyor belt (75) and multiple extrusion sheets (76). The filtering component (11) includes a second pulley (111), a rotating shaft C (112) is provided on the second pulley (111), and a linkage rod (113) is provided on the rotating shaft C (112), and the linkage rod (113) rotates around the rotating shaft C (112). The cleaning component (12) includes a fixed frame (121), a sliding groove (114) is provided on the fixed frame (121), and a slider is slidably connected to the inside of the sliding groove (114), and the bottom surface of the front of the slider is respectively connected to the shaking frame 1 (115) and the shaking frame 2 (116).

2. The integrated reaction device for filtering kinase according to claim 1, characterized in that: The stirring assembly (5) includes a motor 1 (51), a bevel gear 1 (53), a bevel gear 2 (54) and a stirring blade (55), wherein the motor 1 (51) is provided with a rotating shaft A (52), the bevel gear 1 (53) is provided on the rotating shaft A (52), the bevel gear 1 (53) and the bevel gear 2 (54) are meshed, the bevel gear 2 (54) is provided with a stirring shaft, and a plurality of stirring blades (55) are evenly provided on the stirring shaft.

3. The integrated reaction device for filtering kinase according to claim 2, characterized in that: A protective box (6) is provided on the outer sides of the bevel gear 1 (53) and the bevel gear 2 (54), and a position sensor (10) is provided on the slider.

4. The integrated reaction device for filtering kinase according to claim 1, characterized in that: A fixing groove is provided at the bottom of the fixing frame (121), a telescopic rod (122) is provided inside the fixing groove, a cleaning brush 1 (123) and a cleaning brush 2 (124) are provided in front of the fixing frame (121), the rotating shaft C (112) passes through the interior of the fixing frame (121), and the fixing frame (121) is provided on the inner wall of the reaction box (2).

5. The integrated reaction device for filtering kinase according to claim 4, characterized in that: The shaking frame 1 (115) and the shaking frame 2 (116) are both provided with filter screens, and the cleaning brush 1 (123) and the cleaning brush 2 (124) are respectively attached to the shaking frame 1 (115) and the shaking frame 2 (116).

6. The integrated reaction device for filtering kinase according to claim 5, characterized in that: The lengths of the cleaning brush 1 (123) and the cleaning brush 2 (124) correspond to the diameters of the shaking frame 1 (115) and the shaking frame 2 (116), respectively.

7. The integrated reaction device for filtering kinase according to claim 4, characterized in that: The reaction box (2) is provided with a feeding box (4) and a stirring box (3) from top to bottom, respectively. The bottom surface of the stirring box (3) is provided with an outlet (13).

8. The integrated reaction device for filtering kinase according to claim 4, characterized in that: The transmission assembly (9) is arranged on bevel gear 1 (53), and the transmission assembly (9) includes a transmission auger (91) and a barrel-shaped net (92). The transmission auger (91) is arranged inside the barrel-shaped net (92). The bottom of the conveying frame (8) is connected to the barrel-shaped net (92), and the right side of the conveying frame (8) is connected to the stirring box (3). An inlet (14) is provided on the barrel-shaped net (92), and the inlet (14) and the outlet (13) correspond to each other. A biosensor (15) is provided on the inner wall of the stirring box (3).

9. The integrated reaction device for filtering kinase according to claim 1, characterized in that: A controller is provided in front of the device body (1), and the controller is electrically connected to the motor 1 (51), the motor 2 (71) and the position sensor (10) respectively.

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