Culture device for biological stem cells

Abstract

The invention discloses a biological stem cell culture device which comprises a culture cabinet and a cabinet door hinged to the front side of the culture cabinet, at least two sets of supporting plate sets are arranged on the inner side of the culture cabinet in an up-down distribution mode, and the two sets of supporting plate sets divide the culture cabinet into three culture spaces; a bottom frame is arranged below the culture cabinet, a heating assembly and at least three sets of temperature adjusting assemblies are arranged on the inner side of the bottom frame, and the interior of each temperature adjusting assembly is divided into an inner space and an outer space; the multiple supporting plate sets are distributed in the culture cabinet up and down, the culture cabinet can be divided into the multiple independent spaces under the action of the supporting plate sets, the culture cabinet is provided with the heating assembly, the temperature adjusting assembly and the like, and the heating assembly and the temperature adjusting assembly are connected with the independent spaces; the temperature, humidity and gas ratio of each independent space can be adjusted through the work of the heating assembly and the temperature adjusting assembly, so that cell culture under different conditions can be completed at the same time, and the efficiency of a culture experiment is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, in particular to a culture device for biological stem cells. Background Art

[0002] In the field of biological stem cell research and application, the performance of culture devices plays a key role in experimental efficiency and cell culture quality. Currently, the limitations of traditional culture devices are becoming increasingly prominent, restricting the development of stem cell culture work.

[0003] In the field of biological stem cell culture, traditional culture devices often utilize a single culture vessel to cultivate cells. This single-vessel design presents numerous drawbacks, the most notable of which is extremely low space utilization. Within the device, structural limitations make it difficult to create multiple, independent culture spaces tailored to diverse culture needs. In both scientific research and production practice, stem cell culture experiments often require a variety of different conditions. For example, different stem cell types have varying temperature sensitivities. Some may thrive at a constant temperature of 37°C, while others require slightly lower or higher temperatures. Humidity is also crucial. Proper humidity prevents cell dehydration and maintains normal physiological metabolism. Some stem cells may require a high humidity level above 90%, while others thrive in relatively low humidity. Regarding gas concentrations, the ratio of gases such as oxygen and carbon dioxide significantly influences stem cell proliferation and differentiation. Some culture experiments may require high concentrations of carbon dioxide to simulate the microenvironment of specific tissues in the body.

[0004] However, due to the limitations of traditional culture devices, researchers cannot simultaneously conduct multiple culture experiments under different conditions within the same device. They are forced to adopt a sequential culture model. This involves completing the culture process under one condition first, then comprehensively adjusting the culture device parameters, such as the temperature control system, gas supply, and culture medium composition, before starting the next culture process.

[0005] This culture method has many disadvantages. First, the inability to conduct multiple experiments in parallel leads to a significant increase in the time required to culture under a variety of different conditions, which seriously reduces experimental efficiency and increases the time cost of scientific research projects. Second, sequential culture may cause cells to miss the best experimental observation and processing opportunities, affecting the accuracy and reliability of experimental results. In addition, frequent adjustments to the conditions of the culture device also increase the risk of cell contamination during the culture process, thereby affecting the quality of stem cell culture and the success rate of the experiment. With the deepening of biological stem cell research and the expansion of its application, the demand for new culture devices that can achieve parallel culture under a variety of different conditions and improve culture efficiency and quality has become increasingly urgent. Summary of the Invention

[0006] The object of the present invention is to provide a biological stem cell culture device, aiming to improve the problem that existing culture devices are inconvenient to simultaneously complete cell culture under different conditions.

[0007] The present invention is implemented as follows: a biological stem cell culture device includes a culture cabinet and a cabinet door hingedly arranged on the front side of the culture cabinet. At least two sets of supporting plate groups are distributed above and below the inner side of the culture cabinet, and the two sets of supporting plate groups divide the culture cabinet into three culture spaces; a bottom frame is provided below the culture cabinet, and a heating assembly and at least three sets of temperature control assemblies are provided inside the bottom frame. The interior of the temperature control assembly is divided into an inner and outer space. The inner space of the temperature control assembly is connected to an air source and the culture space, and the heating assembly is connected to the outer space of the temperature control assembly via an air pump.

[0008] Preferably, the supporting plate group includes an intermediate plate and second thermal insulation plates distributed on the upper and lower sides of the intermediate plate, the front side surface of the intermediate plate is flush with the front side surface of the second thermal insulation plate, and the remaining side surfaces of the intermediate plate protrude from the remaining side surfaces of the second thermal insulation plate; multiple groups of card slots are provided on the inner side wall of the incubator, and each group has multiple card slots distributed up and down, and the end of the intermediate plate protruding from the second thermal insulation plate is inserted into the card slot.

[0009] Preferably, three access holes are distributed on the cabinet door, and the three access holes are opposite to the three culture spaces one by one, and a flip door is hingedly provided at each access hole; the part of the cabinet door deviating from the access hole contacts the front side of the supporting plate group.

[0010] Preferably, the heating assembly includes a frame, an upper cover plate and a lower cover plate distributed and installed on the upper and lower sides of the frame; at least two guide plates are distributed on the upper and lower sides of the inner side of the frame, the width of the guide plate is equal to the width of the inner space of the frame, the length of the guide plate is less than the length of the inner space of the frame, and at the same time, the ends of the two guide plates away from each other are respectively connected to the two ends of the frame.

[0011] Preferably, an air inlet is provided at one end of the lower cover plate, and an air outlet pipe is provided at the top of the end wall of the frame away from the air inlet, and the air outlet pipe is connected to the air inlet end of the air pump; the air outlet pipe and the air inlet are distributed on the upper and lower sides of the two guide plates, and the air outlet pipe, the air inlet and the two guide plates form an S-shaped flow channel for the gas; a filter device is provided at the air inlet, and the filter device includes a shell, and a top net and a bottom net respectively installed on the upper and lower sides of the shell.

[0012] Preferably, heating wires are provided in the three spaces distributed above and below formed by the two guide plates and the upper cover plate and the lower cover plate. The heating wires are supported on the bracket, and the ends of the heating wires pass through the frame and are connected to the electrical control box. The bracket is installed on the end wall of the frame.

[0013] Preferably, each set of temperature control components includes an outer tube, and pipes are provided at the upper and lower ends of the outer tube, and a first solenoid valve is installed on the upper pipe; the upper and lower pipes of the three sets of temperature control components are respectively connected to the second tube body and the third tube body, the second tube body is connected to the output end of the air pump, and the curved pipe installed at the end of the third tube body extends to the air inlet.

[0014] Preferably, the temperature control component further includes a converging device, which includes a first end plate, a second end plate and a plurality of air pipes; the first end plate and the second end plate are respectively installed at the upper and lower ends of the outer tube, and the plurality of air pipes are all arranged through the outer tube.

[0015] Preferably, a first cavity is provided on the inner side of the first end plate, and a plurality of first end tubes and a first conduit are respectively connected on the upper and lower sides of the first cavity, a second solenoid valve is installed on the first end tube, and the first end tube is connected to the gas source; a second cavity is provided on the inner side of the second end plate, and a plurality of second conduits and a second end tube are respectively connected on the upper and lower sides of the second cavity, and the second end tube is connected to the air inlet pipe installed on the rear side of the culture cabinet; a first sleeve and a second sleeve are respectively provided at both ends of the air pipe, the first sleeve is threadedly sleeved on the first conduit, and the second sleeve is sleeved on the second conduit.

[0016] Preferably, the first end tube is connected to the top of the disinfection assembly through the first tube body, and the bottom of the disinfection assembly is connected to the gas source through the fourth tube body; the disinfection assembly includes a barrel, a lamp holder and an ultraviolet lamp, the lamp holder is installed at the opening of the barrel, the ultraviolet lamp is installed on the lamp holder, and the ultraviolet lamp is located in the barrel.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] 1. The culture cabinet of the present invention is provided with multiple sets of supporting plate groups distributed above and below, which can divide the culture cabinet into multiple independent spaces under the action of the supporting plate groups. The culture cabinet is equipped with a heating component, a temperature control component, etc., and the heating component and the temperature control component are connected to each independent space. The temperature, humidity and gas ratio of each independent space can be adjusted by the operation of the heating component and the temperature control component, so that cell culture under different conditions can be completed at the same time, thereby improving the efficiency of the culture experiment.

[0019] 2. The temperature control component provided in the present invention is composed of an outer tube and a converging device, and the converging device is provided through the outer tube, and then the outer tube is divided into inner and outer spaces under the action of the converging device. The hot gas flowing in from the heating component is located in the outer space of the converging device, and the gas flowing in from the gas source is located in the inner space of the converging device. The gas flowing in from the gas source is heated by the principle of heat conduction, and then flows into each independent space to achieve temperature and humidity regulation. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic structural diagram of the present invention as a whole;

[0021] Figure 2 It is a first structural schematic diagram of the culture cabinet of the present invention;

[0022] Figure 3 It is a structural schematic diagram of the supporting plate assembly of the present invention;

[0023] Figure 4 It is a partial structural diagram of the cabinet door of the present invention;

[0024] Figure 5 is a second structural schematic diagram of the culture cabinet of the present invention;

[0025] Figure 6 This is a first structural diagram of the bottom frame, temperature regulating assembly, disinfection assembly, and heating assembly of the present invention;

[0026] Figure 7 This is a second structural diagram of the bottom frame, temperature control component, disinfection component, and heating component of the present invention;

[0027] Figure 8 It is a schematic structural diagram of the heating assembly of the present invention;

[0028] Figure 9 It is a schematic structural diagram of the heating wire of the present invention;

[0029] Figure 10 It is a structural diagram of the frame, lower cover and upper cover of the present invention;

[0030] Figure 11 It is a partial structural schematic diagram of the lower cover plate of the present invention;

[0031] Figure 12 It is a structural schematic diagram of the filter device of the present invention;

[0032] Figure 13 It is a structural schematic diagram of the temperature control component and the disinfection component of the present invention;

[0033] Figure 14 It is a schematic structural diagram of the disinfection assembly of the present invention;

[0034] Figure 15 It is a structural schematic diagram of the temperature control component of the present invention;

[0035] Figure 16 It is a structural schematic diagram of the outer tube of the present invention;

[0036] Figure 17 It is a structural schematic diagram of the convergence device of the present invention;

[0037] Figure 18 It is a schematic structural diagram of the second end plate of the present invention;

[0038] Figure 19It is a schematic structural diagram of the first end plate of the present invention;

[0039] Figure 20 It is a structural schematic diagram of the trachea of the present invention.

[0040] In the figure: 1. Incubator; 11. Cabinet door; 12. Exhaust hole; 13. Air inlet pipe; 14. Card slot; 15. Inlet and outlet hole; 16. Flip door; 17. Inner plate; 18. First heat insulation board; 19. Outer plate; 2. Support plate group; 21. Middle plate; 22. Second heat insulation board; 3. Bottom frame; 4. Temperature control component; 41. First tube body; 42. Second tube body; 43. Third tube body; 44. Outer tube; 441. First solenoid valve; 5. Disinfection component; 51. Fourth tube body; 52. UV lamp; 53. Lamp holder; 54. Cylinder body; 6. Heating component ;61. Frame;62. Heating wire;621. Bracket;63. Guide plate;64. Lower cover;641. Air inlet;65. Upper cover;66. Filter device;661. Shell;662. Bottom net;663. Top net;7. Air pump;8. Electrical control box;9. Converging device;91. Air pipe;911. First sleeve;912. Second sleeve;92. First end plate;921. First cavity;922. First conduit;923. Second solenoid valve;93. Second end plate;931. Second cavity;932. Second conduit. DETAILED DESCRIPTION

[0041] In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," "connect," "fixed," etc. should be understood broadly. For example, they may refer to fixed connection, detachable connection, or integration; mechanical connection or electrical connection; direct connection or indirect connection through an intermediate medium; internal communication between two components or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0042] The following is a further description with reference to the accompanying drawings and specific embodiments:

[0043] like Figure 1 、 Figure 6 、 Figure 7As shown, in order to simultaneously complete cell culture under different culture conditions, reduce the time spent on different culture conditions, and improve the efficiency of culture experiments, this embodiment provides a new culture device. The culture device includes a culture cabinet 1 and a cabinet door 11 hingedly mounted on the front side of the culture cabinet 1. The culture cabinet 1 is divided into multiple independent spaces, and cells under different culture conditions can be placed in different spaces. In order to create different culture environments in multiple independent spaces, the culture cabinet 1 needs to be equipped with corresponding heating components 6, temperature control components 4, etc. The interior of the temperature control component 4 is divided into two spaces, the inner and outer spaces. The inner space of the temperature control component 4 is connected to the gas source (oxygen, carbon dioxide, etc.) and the culture space. A humidification bottle can be added to the pipeline connecting the temperature control component 4 and the gas source. The humidification bottle adjusts the humidity of the gas, and while adjusting the ratio of oxygen, carbon dioxide, etc. in the culture space, the humidity of the culture space is also adjusted. In addition, the heating component 6 is connected to the outer space of the temperature control component 4 through an air pump 7. The air pump 7 can be used to transport the gas heated by the heating component 6 to the temperature control component 4. The heat conduction principle is used to increase the gas flowing through the temperature control component 4, thereby adjusting the temperature in the culture space. In order to be able to adjust the temperature, humidity, and the ratio of gases such as oxygen and carbon dioxide, temperature and humidity sensors, oxygen, carbon dioxide, etc. gas concentration sensors, etc., need to be installed in each culture space, and each sensor is connected to the electrical control box 8.

[0044] Through the above technical solution, spaces with different temperatures, humidity and gas concentrations can be created in each culture space according to needs, and then in a single culture process in the culture cabinet 1, stem cells can be cultured under multiple different conditions, thereby correspondingly improving the culture efficiency.

[0045] like Figure 3 As shown, in order to form multiple independent spaces in the culture cabinet 1, it is necessary to install multiple supporting plate groups 2 in the culture cabinet 1. In order to facilitate the introduction of the specific technical solution, in this embodiment, the supporting plate groups 2 are set as two sets, and each set of supporting plate groups 2 includes an intermediate plate 21 and a second thermal insulation plate 22 distributed on the upper and lower sides of the intermediate plate 21. The front side surface of the intermediate plate 21 is flush with the front side surface of the second thermal insulation plate 22, and the remaining side surfaces of the intermediate plate 21 protrude from the remaining side surfaces of the second thermal insulation plate 22. The setting of the intermediate plate 21 is mainly to increase the higher strength so that the culture dish can be stably supported by the supporting plate group 2. The thermal insulation plate can be made of thermal insulation materials such as rock wool and glass wool. Its purpose is to block the heat flow transfer and reduce the temperature transfer between adjacent culture spaces.

[0046] like Figure 1 、 Figure 3As shown, to stably mount the support plate assembly 2 within the incubator 1, multiple sets of slots 14 are provided on the inner sidewall of the incubator 1. Each set includes multiple slots 14 arranged vertically. The ends of the intermediate plate 21 protruding from the second thermal insulation plate 22 are inserted into the slots 14, thereby stably mounting the support plate assembly 2 within the incubator 1 and facilitating removal and repositioning of the support plate assembly 2 as needed. The two sets of support plate assemblies 2 divide the incubator 1 into three independent culture spaces. Furthermore, the portion of the cabinet door 11 that deviates from the access opening 15 contacts the front side of the support plate assembly 2, thereby forming an isolated culture space in cooperation with the support plate assembly 2.

[0047] like Figure 4 As shown, in order to be able to take and place the culture dishes in a certain culture space according to needs, three access holes 15 are distributed on the cabinet door 11. The three access holes 15 are directly opposite to the three culture spaces, and a flip door 16 is hingedly provided at each access hole 15, which can facilitate opening and closing the flip door 16 to adjust the smoothness of the access hole 15, providing convenience for taking and placing the culture dishes in each space.

[0048] like Figure 5 As shown, in order to reduce heat loss in each space, the side walls of the incubator 1 and the cabinet door 11 include an inner plate 17, a first thermal insulation plate 18 and an outer plate 19. The first thermal insulation plate 18 is located between the inner plate 17 and the outer plate 19. The thermal insulation plate can block the heat flow transfer and reduce the temperature transfer between the incubator 1 and the external space.

[0049] like Figure 6 、 Figure 7 、 Figure 13As shown, in order to realize the regular distribution of the heating components 6 and the temperature control components 4, a bottom frame 3 is provided at the bottom of the incubator 1, and the heating components 6 and the temperature control components 4 are arranged on the inner side of the bottom frame 3. In order to realize the connection between the heating components 6, the temperature control components 4 and the incubator 1. The sides of the three sets of temperature control components 4 are connected with a second tube body 42 and a third tube body 43, the second tube body 42 is connected to the output end of the air pump 7, the air inlet end of the air pump 7 is connected to the air outlet pipe, and the air outlet pipe is connected to the heating component 6. In addition, the bend installed at the end of the third tube body 43 extends to the air inlet hole 641 of the heating component 6, so that the heated gas can be pumped to the outer space of the temperature control component 4 under the action of the air pump 7, and then transported from the end of the third tube body 43 to the air inlet hole 641, so as to realize the heating treatment of the gas flowing through the temperature control component 4. The air inlet end of the temperature control component 4 is connected to the top of the disinfection component 5 via a first tube 41, and the bottom of the disinfection component 5 is connected to the air source via a fourth tube 51. This allows the disinfection of the gas through the disinfection component 5, preventing the gas from entering the culture cabinet 1 and causing contamination. Furthermore, the air outlet pipe of the temperature control component 4 is connected to the air inlet pipe 13 on the rear side of the culture cabinet 1 via a pipeline, and the air inlet pipe 13 is arranged to be directly opposite and communicate with the three culture spaces. Therefore, the gas flowing through the temperature control component 4 is heated and then flows into each culture space of the culture cabinet 1. When the gas environment is satisfied, the temperature of the culture space can be increased. To prevent the pressure in the culture space from increasing, exhaust holes 12 are also provided on the side walls of each culture space.

[0050] like Figure 15 As shown, in order to form two inner and outer spaces inside the temperature control component 4 to realize the heating treatment of the gas, it includes an outer tube 44, a converging device 9, etc. The converging device 9 is arranged through the outer tube 44, and under the action of the converging device 9, the outer tube 44 is divided into two inner and outer spaces. The oxygen, carbon dioxide and other gases flowing through the temperature control component 4 pass through the space inside the converging device 9, and the gas input from the heating component 6 passes through the space outside the converging device 9, so that the heat transfer of the gas can be realized through the heat transfer of the converging device 9.

[0051] like Figure 16 As shown, specifically, pipes are provided at both ends of the outer tube 44, a first solenoid valve 441 is installed on the upper pipe, and the pipes distributed above and below are connected to the second tube body 42 and the third tube body 43 respectively, and the second tube body 42 is connected to the output end of the air pump 7.

[0052] like Figure 17 As shown, the converging device 9 includes a first end plate 92, a second end plate 93 and a plurality of air pipes 91. The first end plate 92 and the second end plate 93 are respectively installed at the upper and lower ends of the outer tube 44. The plurality of air pipes 91 (made of heat-conducting materials such as copper and aluminum) are all set through the outer tube 44.

[0053] like Figure 18-20 As shown, a first cavity 921 is provided on the inner side of the first end plate 92. Multiple first end tubes and a first conduit 922 are connected to the upper and lower sides of the first cavity 921. A second solenoid valve 923 is installed on the first end tube. The first end tube is connected to the top of the disinfection assembly 5 via the first tube body 41, and the bottom of the disinfection assembly 5 is connected to the air source via the fourth tube body 51. A second cavity 931 is provided on the inner side of the second end plate 93. Multiple second conduits 932 and second end tubes are connected to the upper and lower sides of the second cavity 931. The second end tube is connected to the air inlet pipe 13 installed on the rear side of the incubator 1. A first sleeve 911 and a second sleeve 912 are provided at each end of the air pipe 91. The first sleeve 911 is threadedly mounted on the first conduit 922, and the second sleeve 912 is mounted on the second conduit 932. Gases such as oxygen and carbon dioxide flow through the fourth tube 51 and the first tube 41 into the converging device 9, then exit through the second end tube and the air inlet pipe 13 and flow into the incubator 1. During this process, the gases output from the heating assembly 6 surround the outer side of the air pipe 91, allowing them to be heated by the air pipe 91. To adjust the temperature within the incubation space as needed, the flow of heated gases is controlled by the first solenoid valve 441. To adjust the concentration of gases such as oxygen and carbon dioxide, the flow rate of each gas is adjusted by controlling the second solenoid valve 923.

[0054] like Figure 8 、 Figure 10 As shown, in order to provide support for temperature regulation of different culture spaces, the heating assembly 6 includes a frame 61, an upper cover plate 65 and a lower cover plate 64 installed on the upper and lower sides of the frame 61. At least two guide plates 63 are arranged on the upper and lower sides of the inner side of the frame 61. The width of the guide plates 63 is equal to the width of the space inside the frame 61, and the length of the guide plates 63 is less than the length of the space inside the frame 61. At the same time, the ends of the two guide plates 63 that are away from each other are respectively connected to the two ends of the frame 61. Therefore, the two guide plates 63 form a gap with the end wall of the frame 61, thereby forming an S-shaped flow channel inside the frame 61.

[0055] like Figure 10 、 Figure 11 As shown, in addition, an air inlet hole 641 is provided at one end of the lower cover plate 64, and the air inlet hole 641 is set away from the gap near the end of the guide plate 63. An air outlet pipe is provided on the top of the end wall of the frame 61 away from the air inlet hole 641. Similarly, the air outlet pipe is set away from the gap near the end of the guide plate 63. The air outlet pipe and the air inlet hole 641 are distributed on the upper and lower sides of the two guide plates 63. The air outlet pipe is connected to the air inlet end of the air pump 7. This arrangement can pump external gas into the heating component 6 under the action of the air pump 7 and extend the gas movement path in the heating component 6.

[0056] like Figure 8 、 Figure 9 As shown, heating wires 62 are provided in the three spaces distributed above and below formed by the two guide plates 63, the upper cover plate 654 and the lower cover plate 64. The heating wires 62 are supported on the bracket 621, and the end of the heating wire 62 passes through the frame 61 and is connected to the electrical control box 8. The bracket 621 is installed on the end wall of the frame 61 and contacts the heating wire 62 during the gas circulation process, thereby increasing the temperature of the gas.

[0057] like Figure 12 As shown, in order to prevent impurities from entering the heating component 6, a filter device 66 is provided at the air inlet 641. The filter device 66 includes a shell 661, a top net 663 and a bottom net 662 respectively installed on the upper and lower sides of the shell 661. The aperture of the top net 663 is smaller than the aperture of the bottom net 662.

[0058] like Figure 14 As shown, in order to achieve gas disinfection, the disinfection component 5 includes a cylinder 54, a lamp holder 53 and an ultraviolet lamp 52. The lamp holder 53 is installed at the opening of the cylinder 54, and the ultraviolet lamp 52 is installed on the lamp holder 53. The ultraviolet lamp 52 is located in the cylinder 54. The gas entering and exiting the cylinder 54 must pass through the ultraviolet lamp 52, and then the gas is disinfected under the action of the ultraviolet lamp 52.

[0059] In order to control the operation of the air pump 7, solenoid valve, ultraviolet lamp 52, heating wire 62, etc., and receive information from the sensor, the electrical control box 8 is provided with at least a controller, a switching power supply, an input and output module, a relay, a protection device, a sensor interface circuit, a communication module, etc.

[0060] The controller can be configured as a programmable logic controller (PLC), which offers advantages such as high reliability, flexible programming, and powerful functionality. It processes various input signals according to pre-programmed logic and outputs control signals to drive devices such as air pumps, solenoid valves, UV lamps, and heating wires. It is suitable for more complex control logic and the coordinated control of multiple devices.

[0061] Input and output modules can be configured as either digital input or digital output modules. Digital input modules receive digital signals from sensors and convert them into digital signals that the controller can interpret for logical analysis and processing. Digital output modules, based on controller instructions, output digital signals to control the on / off states of devices such as air pumps, solenoid valves, and UV lamps. For example, a high or low level output controls the opening and closing of a solenoid valve, thereby enabling on / off control of gas or liquid.

[0062] Relays can be configured as intermediate relays to increase the number and capacity of contacts in a control circuit, thereby expanding the controller's control capabilities. For example, when a single controller output point needs to control multiple devices or switch different circuits, this can be achieved using an intermediate relay.

[0063] Protective devices include fuses, circuit breakers, surge protectors, etc.

[0064] The sensor interface circuit includes signal conditioning and isolation circuits. The signal conditioning circuit amplifies, filters, and linearizes the sensor output signal to meet the controller's input requirements. For example, it amplifies weak sensor signals to an appropriate level, filters out noise interference, and improves signal quality and stability. The isolation circuit uses technologies such as optoelectronic isolation or magnetic isolation to isolate the electrical signals between the sensor and the controller, preventing external interference signals from entering the controller and protecting the sensor from electrical interference and faults on the controller side.

[0065] The communication module can be set as a serial communication module, such as RS232, RS485, etc., which can realize serial data communication between the controller and other devices (such as host computer, sensor, etc.), and is suitable for short-distance, low-speed data transmission.

[0066] The above is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that the present invention is susceptible to various modifications and variations. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A biological stem cell culture device, characterized in that: The invention comprises a culture cabinet (1) and a cabinet door (11) hingedly arranged on the front side of the culture cabinet (1); at least two sets of supporting plate groups (2) are distributed on the upper and lower sides of the inner side of the culture cabinet (1); the two sets of supporting plate groups (2) divide the culture cabinet (1) into three culture spaces; a bottom frame (3) is arranged below the culture cabinet (1); a heating component (6) and three sets of temperature control components (4) are arranged on the inner side of the bottom frame (3); the interior of the temperature control component (4) is divided into an inner and outer space; the inner space of the temperature control component (4) is connected to an air source and a culture space; the heating component (6) is connected to the outer space of the temperature control component (4) via an air pump (7).

2. A biological stem cell culture device according to claim 1, characterized in that: The supporting plate group (2) comprises an intermediate plate (21) and second heat-insulating plates (22) distributed on the upper and lower sides of the intermediate plate (21); the front side surface of the intermediate plate (21) is flush with the front side surface of the second heat-insulating plate (22); and the remaining side surfaces of the intermediate plate (21) protrude from the remaining side surfaces of the second heat-insulating plate (22); a plurality of groups of card slots (14) are provided on the inner side wall of the culture cabinet (1), and each group includes a plurality of card slots (14) distributed vertically, and the end portion of the intermediate plate (21) protruding from the second heat-insulating plate (22) is inserted into the card slot (14).

3. A biological stem cell culture device according to claim 2, characterized in that: The cabinet door (11) is provided with three inlet and outlet holes (15) distributed thereon, the three inlet and outlet holes (15) are directly opposite to the three culture spaces, and a flip door (16) is hingedly provided at each of the inlet and outlet holes (15); the portion of the cabinet door (11) that deviates from the inlet and outlet holes (15) contacts the front side of the supporting plate group (2).

4. The biological stem cell culture device according to claim 1, characterized in that: The heating assembly (6) comprises a frame (61), an upper cover plate (65) and a lower cover plate (64) which are distributed and installed on the upper and lower sides of the frame (61); at least two guide plates (63) are distributed and arranged on the upper and lower sides of the inner side of the frame (61); the width of the guide plates (63) is equal to the width of the inner space of the frame (61), and the length of the guide plates (63) is less than the length of the inner space of the frame (61); at the same time, the ends of the two guide plates (63) that are away from each other are respectively connected to the two ends of the frame (61).

5. The biological stem cell culture device according to claim 4, characterized in that: An air inlet (641) is provided at one end of the lower cover plate (64), and an air outlet pipe is provided at the top of the end wall of the frame (61) away from the air inlet (641), and the air outlet pipe is connected to the air inlet end of the air pump (7); the air outlet pipe and the air inlet (641) are distributed on the upper and lower sides of the two guide plates (63), and the air outlet pipe, the air inlet (641) and the two guide plates (63) form an S-shaped flow channel for gas; a filter device (66) is provided at the air inlet (641), and the filter device (66) includes a shell (661), a top net (663) and a bottom net (662) respectively installed on the upper and lower sides of the shell (661).

6. The biological stem cell culture device according to claim 5, characterized in that: Heating wires (62) are provided in three spaces distributed above and below formed by the two guide plates (63), the upper cover plate (65) and the lower cover plate (64). The heating wires (62) are supported on a bracket (621), and the ends of the heating wires (62) pass through the frame (61) and are connected to the electrical control box (8). The bracket (621) is installed on the end wall of the frame (61).

7. The biological stem cell culture device according to claim 5, characterized in that: Each set of the temperature control components (4) includes an outer tube (44), and pipes are provided at both upper and lower ends of the outer tube (44), and a first solenoid valve (441) is installed on the upper pipe; the upper and lower pipes of the three sets of temperature control components (4) are respectively connected to a second pipe body (42) and a third pipe body (43), the second pipe body (42) is connected to the output end of the air pump (7), and a bend pipe installed at the end of the third pipe body (43) extends to the air inlet hole (641).

8. The biological stem cell culture device according to claim 7, characterized in that: The temperature control assembly (4) further comprises a convergence device (9), the convergence device (9) comprising a first end plate, a second end plate and a plurality of air pipes (91); the first end plate and the second end plate are respectively mounted at the upper and lower ends of the outer tube (44), and the plurality of air pipes (91) are all arranged to penetrate the outer tube (44).

9. The biological stem cell culture device according to claim 8, characterized in that: A first cavity (921) is provided on the inner side of the first end plate, and multiple first end tubes and a first conduit (922) are connected to each other on the upper and lower sides of the first cavity (921), a second solenoid valve (923) is installed on the first end tube, and the first end tube is connected to the gas source; a second cavity (931) is provided on the inner side of the second end plate, and multiple second conduits (932) and a second end tube are connected to each other on the upper and lower sides of the second cavity (931), and the second end tube is connected to the air inlet pipe (91) (13) installed on the rear side of the culture cabinet (1); a first sleeve (911) and a second sleeve (912) are provided at both ends of the air pipe (91), the first sleeve (911) is threadedly sleeved on the first conduit (922), and the second sleeve (912) is sleeved on the second conduit (932).

10. The biological stem cell culture device according to claim 9, characterized in that: The first end tube is connected to the top of the disinfection component (5) through the first tube body (41), and the bottom of the disinfection component (5) is connected to the gas source through the fourth tube body (51); the disinfection component (5) comprises a barrel (54), a lamp holder (53) and an ultraviolet lamp (52), wherein the lamp holder (53) is installed at the opening of the barrel (54), the ultraviolet lamp (52) is installed on the lamp holder (53), and the ultraviolet lamp (52) is located inside the barrel (54).

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