Method for promoting microalgae growth by using pharmaceutical product

Abstract

The invention belongs to the technical field of microalgae biology and culture, and particularly relates to a method for promoting microalgae growth by using a pharmaceutical product, which comprises the following steps: activating microalgae on a solid flat plate, inoculating the activated microalgae into a liquid culture medium, adding the pharmaceutical product at the initial stage of microalgae growth, placing in a constant-temperature shaking table, and carrying out illumination culture. According to the method, different types of pharmaceutical products with different concentrations are added into the microalgae, the influence of the addition of the different types of pharmaceutical products with different concentrations on the growth promotion of the microalgae is analyzed, the biomass of the chlorella can be rapidly increased, and the method has important significance on the large-scale production of the microalgae.

Description

Technical Field

[0001] The invention belongs to the technical field of microalgae biology and cultivation, and particularly relates to a method for promoting the growth of microalgae by utilizing pharmaceutical products. Background Art

[0002] There are many types of microalgae, which are widely distributed on land and in the ocean. They have high photosynthesis efficiency, can efficiently utilize light energy, and absorb CO2 to convert it into nutrients such as proteins, polysaccharides and lipids. They have good development prospects in the fields of food, medicine, energy, environmental protection, etc.

[0003] Growth rate and production efficiency are key factors in producing high-value-added products from microalgae. However, current production processes and technologies still have limitations in microalgae growth and product synthesis, resulting in long production cycles and limited output of added products. Microalgae absorb carbon dioxide through photosynthesis, but their limited growth rate and scale make it difficult to sequester enough carbon dioxide to meet the needs of large-scale emissions reductions. Accelerating microalgae growth can increase their biomass and photosynthetic efficiency, thereby sequestering more carbon dioxide per unit time and making a greater contribution to mitigating the greenhouse effect.

[0004] Traditional methods for promoting microalgae growth, such as optimizing culture conditions like light, temperature, and nutrients, have achieved some success but are facing bottlenecks. Therefore, finding a low-cost, efficient, and environmentally friendly method for promoting microalgae growth is of great practical significance. Summary of the Invention

[0005] In response to the shortcomings of the above-mentioned existing technologies, the present invention provides a method for promoting the growth of microalgae using pharmaceutical products. By adding different types and concentrations of pharmaceutical products to the microalgae, the effects of the addition of different types and concentrations of pharmaceutical products on the growth promotion of the microalgae are analyzed. This method can rapidly increase the biomass of Chlorella vulgaris, which is of great significance for the large-scale production of microalgae.

[0006] The specific technical solutions are as follows: A method for promoting the growth of microalgae using pharmaceutical products comprises the following steps: activating the microalgae on a solid plate, inoculating the activated microalgae into a liquid culture medium, adding the pharmaceutical product at the early stage of microalgae growth, placing the microalgae in a constant temperature shaker, and culturing under light.

[0007] Furthermore, the added concentration of the pharmaceutical product is 0.1~30 mg / L.

[0008] Furthermore, the added concentration of the pharmaceutical product is preferably 5 mg / L, 10 mg / L, 20 mg / L, 30 mg / L, and more preferably 20 mg / L.

[0009] Furthermore, the initial OD of the microalgae680 The luminance is 0.1-0.3, the shaking speed is 80-150 rpm, the illumination intensity is 1000-3000 lux, and the culture temperature is 25-30°C.

[0010] Furthermore, the pharmaceutical product is one or more of steroids, fluoroquinolone antibiotics, sulfonamide antibiotics or non-steroidal anti-inflammatory drugs.

[0011] Furthermore, the steroid drug is prednisone and / or dexamethasone, preferably dexamethasone.

[0012] Furthermore, the fluoroquinolone antibiotic is one or more of norfloxacin, enoxacin, fleroxacin, ofloxacin, ciprofloxacin or lomefloxacin, preferably norfloxacin.

[0013] Furthermore, the sulfonamide antibiotic is one or more of sulfamethazine, sulfamethoxazole, sulfisoxazole, sulfacetamide or sulfadiazine, preferably sulfisoxazole.

[0014] Furthermore, the nonsteroidal anti-inflammatory drug is one or more of diclofenac, ibuprofen or indomethacin, preferably indomethacin.

[0015] Furthermore, the microalgae is Chlorella vulgaris, the solid plate is a BG11 solid plate, and the culture medium is BG11 culture medium.

[0016] Furthermore, the activation comprises the following steps: using an inoculation loop to pick up the preserved Chlorella liquid, streaking and culturing on a BG11 solid plate; picking a single algae colony grown on the plate, inoculating it into a liquid culture medium BG11 medium for growth, and expanding the culture after the algae grows to a dark green color. The expanded Chlorella is activated Chlorella.

[0017] Compared with the prior art, the present invention has the following beneficial effects: (1) The present invention can effectively promote the growth of microalgae, rapidly increase the biomass of microalgae, and realize large-scale production of microalgae by adding low concentrations of different pharmaceutical products to the microalgae culture system; (2) The present invention uses different pharmaceutical products as growth regulators for Chlorella, effectively increasing the biomass of Chlorella, which is of great significance to the large-scale production of Chlorella; (3) The method of the present invention using pharmaceutical products to promote the growth of microalgae can significantly promote the growth of Chlorella vulgaris and achieve the best promotion effect when the concentration of the pharmaceutical product is 20 mg / L; (4) The pharmaceutical product mixture of the present invention will affect the growth of Chlorella. The mixture containing different pharmaceutical pollutants can significantly promote the growth of Chlorella at a lower concentration through synergistic effects. (5) The method of the present invention using pharmaceutical products to promote the growth of microalgae can, on the one hand, realize the resource utilization of pharmaceutical by-products, reduce environmental pollution, be environmentally friendly, and reduce processing costs; on the other hand, it provides a new strategy for large-scale cultivation of microalgae, reduces production costs, improves the market competitiveness of microalgae-related products, and promotes the development of related industries. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 OD of Examples 1 to 4 of the present invention and Comparative Example 1 680 Graph of changes over time; Figure 2 The biomass results of Examples 1 to 4 of the present invention and Comparative Example 1 are shown; Figure 3 These are photos of Examples 1 to 4 of the present invention and Comparative Example 1; Figure 4 The OD values of prednisone and dexamethasone in the steroid drugs added by the present invention are 680 Graph of changes over time; Figure 5 The OD values of norfloxacin, enoxacin, fleroxacin, ofloxacin, ciprofloxacin and lomefloxacin in the fluoroquinolone antibiotics are added to the present invention. 680 Graph of changes over time; Figure 6 The OD value of sulfadimethazine, sulfamethoxazole, sulfisoxazole, sulfacetamide and sulfadiazine in the sulfonamide antibiotics is added to the present invention. 680 Graph of changes over time; Figure 7 The OD of diclofenac, ibuprofen and indomethacin in the non-steroidal anti-inflammatory drugs of the present invention is added 680 Graph of changes over time; Figure 8 The OD of dexamethasone added to the present invention 680 Curve graph showing changes with concentration; Figure 9 The OD of the present invention is added with sulfisoxazole 680 The curve of the change with concentration; Figure 10 The OD of norfloxacin added to the present invention 680 The curve of the change with concentration; Figure 11 The OD of indomethacin added to the present invention 680 The curve of the change with concentration; Figure 12 OD of the mixed preparation composed of different pharmaceutical products added in the present invention 680 Graph of changes over time. DETAILED DESCRIPTION

[0019] The principles and features of the present invention are described below with reference to examples. The examples are only used to explain the present invention and are not used to limit the scope of the present invention. The parts described in the specific implementation manner are parts by weight.

[0020] In a specific embodiment, the Chlorella pyrenoidosa GY-D26 used was isolated and purified by the laboratory of the School of Pharmacy of Binzhou Medical College. The specific process is as follows: a Chlorella suspension was obtained by sampling from Jixia Lake of Shandong University of Technology; the Chlorella suspension was gradiently diluted with sterile water to 10 -8 concentration; take 0.1 mL of the dilution and drop it onto a BG11 solid culture medium plate, and use a sterile applicator to evenly spread the bacterial solution on the surface of the plate to ensure that the bacterial solution is evenly distributed; the inoculated plate is placed upside down in an incubator with a light intensity of 2000 lux, a temperature of 25°C, and a photoperiod of 12 hours light / 12 hours dark. Culture for one week until a single algal colony grows; use a sterile inoculation loop to pick a single colony, inoculate it onto a new BG11 solid culture medium plate, and streak the plate again. Repeat this 2-3 times to obtain a purified Chlorella strain; after obtaining the strain, extract the genome for strain identification; after identification, the obtained strain is Chlorella pyrenoidosa GY-D26.

[0021] All chemical reagents used were commercially available products.

[0022] The absorbance value of Chlorella was measured at an ultraviolet absorption wavelength of 680 nm using an ultraviolet spectrophotometer, so the growth effect of Chlorella was judged based on the change in the absorbance value of Chlorella at 680 nm.

[0023] Example 1 A method for promoting the growth of Chlorella using a pharmaceutical product comprises the following steps: activating Chlorella on a BG11 solid plate, picking up a stored Chlorella liquid with an inoculation loop, and streaking the plate for culture; picking up a single algae colony grown on the plate, inoculating it into a liquid culture medium BG11, growing it, and expanding it after it grows to a dark green color, so that the expanded Chlorella is activated Chlorella; inoculating the activated Chlorella into the liquid culture medium BG11, and the initial OD value of the Chlorella is 0.04. 680 The concentration of 0.15 was set, and 20 mg / L of the pharmaceutical steroid drug prednisone was added at the early stage of the growth of Chlorella. The culture was placed in a constant temperature shaker at 80 rpm, with a light intensity of 1000 lux and cultured at 25°C for 12 days. Samples were taken every 2 days to measure the absorbance of the algae solution.

[0024] Examples 2-4 A method for promoting the growth of Chlorella vulgaris using pharmaceutical products, referring to Example 1, wherein the steroid drug prednisone in Example 1 is replaced in sequence with the fluoroquinolone antibiotic enoxacin, the sulfonamide antibiotic sulfacetamide, and the non-steroidal anti-inflammatory drug ibuprofen, and the rest is the same as in Example 1.

[0025] Comparative Example 1 A method for growing Chlorella vulgaris is described with reference to Example 1, except that the steroid drug prednisone is deleted from Example 1. The remaining steps are the same as those in Example 1.

[0026] Example 5 A method for promoting the growth of Chlorella using a pharmaceutical product comprises the following steps: activating Chlorella on a BG11 solid plate, picking up a stored Chlorella liquid with an inoculation loop, and streaking the plate for culture; picking up a single algae colony grown on the plate, inoculating it into a liquid culture medium BG11, growing it, and expanding it after it grows to a dark green color, so that the expanded Chlorella is activated Chlorella; inoculating the activated Chlorella into the liquid culture medium BG11, and the initial OD value of the Chlorella is 0.04. 680 The concentration of 1g / L prednisone (a pharmaceutical steroid drug) was 0.15, and 10 mg / L of prednisone was added at the early stage of the growth of Chlorella vulgaris. The culture was placed in a constant temperature shaker at 150 rpm, with a light intensity of 3000 lux and cultured at 30°C for 12 days. Samples were taken every 2 days to measure the absorbance of the algae solution.

[0027] Examples 6 to 20 A method for promoting the growth of Chlorella vulgaris using pharmaceutical products, referring to Example 5, wherein the steroid drug prednisone in Example 5 is replaced with dexamethasone, the fluoroquinolone antibiotics include norfloxacin, enoxacin, fleroxacin, ofloxacin, ciprofloxacin, and lomefloxacin, the sulfonamide antibiotics include sulfamethazine, sulfamethoxazole, sulfisoxazole, sulfacetamide, and sulfadiazine, and the nonsteroidal anti-inflammatory drugs include diclofenac, ibuprofen, and indomethacin. The rest of the ingredients are the same as in Example 5.

[0028] Comparative Example 2 A method for growing Chlorella vulgaris is described with reference to Example 5, except that the steroid drug prednisone is deleted. The remaining steps are the same as those described in Example 5.

[0029] Example 21 A method for promoting the growth of Chlorella using a pharmaceutical product comprises the following steps: activating Chlorella on a BG11 solid plate, picking up a stored Chlorella liquid with an inoculation loop, and streaking the plate for culture; picking up a single algae colony grown on the plate, inoculating it into a liquid culture medium BG11, growing it, and expanding it after it grows to a dark green color, so that the expanded Chlorella is activated Chlorella; inoculating the activated Chlorella into the liquid culture medium BG11, and the initial OD value of the Chlorella is 0.04.680 The concentration of the steroid drug dexamethasone was 0.3, and 5 mg / L of the pharmaceutical product dexamethasone was added at the early stage of the growth of Chlorella. The culture was placed in a constant temperature shaker at 120 rpm, with a light intensity of 2000 lux and cultured at 28°C for 12 days. Samples were taken every 2 days to measure the absorbance of the algae solution.

[0030] Examples 22-24 A method for promoting the growth of Chlorella using a pharmaceutical product, referring to Example 21, wherein the addition concentrations in Example 21 are replaced with 10 mg / L, 20 mg / L, and 30 mg / L, respectively, and the rest are the same as in Example 21.

[0031] Comparative Example 3 A method for growing Chlorella vulgaris is described with reference to Example 21, except that the steroid drug prednisone is deleted from Example 21. The remaining steps are the same as those described in Example 21.

[0032] Comparative Examples 4 and 5 A method for growing Chlorella, referring to Example 21, wherein the addition concentrations in Example 21 are replaced with 0.5 mg / L and 40 mg / L, respectively, and the rest are the same as in Example 21.

[0033] Example 25 A method for promoting the growth of Chlorella vulgaris using a pharmaceutical product, referring to Example 21, except that the steroid drug dexamethasone in Example 21 is replaced with the sulfonamide antibiotic sulfisoxazole, and the rest is the same as Example 21.

[0034] Examples 26-28 A method for promoting the growth of Chlorella using a pharmaceutical product, referring to Example 25, wherein the addition concentrations in Example 25 are replaced with 10 mg / L, 20 mg / L, and 30 mg / L, respectively, and the rest are the same as in Example 25.

[0035] Comparative Examples 6 and 7 A method for growing Chlorella, referring to Example 25, wherein the addition concentrations in Example 25 are replaced with 0.5 mg / L and 40 mg / L, respectively, and the rest are the same as in Example 25.

[0036] Example 29 A method for promoting the growth of Chlorella vulgaris using a pharmaceutical product, referring to Example 21, except that the steroid drug dexamethasone in Example 21 is replaced with the fluoroquinolone antibiotic norfloxacin, and the rest is the same as Example 21.

[0037] Examples 30-32 A method for promoting the growth of Chlorella using a pharmaceutical product, referring to Example 29, except that the added concentrations in Example 29 are replaced with 10 mg / L, 20 mg / L, and 30 mg / L, respectively, and the rest are the same as in Example 29.

[0038] Comparative Examples 8 and 9 A method for growing Chlorella is described, with reference to Example 29, except that the addition concentrations in Example 29 are replaced with 0.5 mg / L and 40 mg / L, respectively. The remainder is the same as in Example 29.

[0039] Example 33 A method for promoting the growth of Chlorella vulgaris using a pharmaceutical product, referring to Example 21, except that the steroid drug dexamethasone in Example 21 is replaced with the non-steroidal anti-inflammatory drug indomethacin, and the rest of the steps are the same as in Example 21.

[0040] Examples 34-36 A method for promoting the growth of Chlorella using a pharmaceutical product, referring to Example 33, except that the added concentrations in Example 33 are replaced with 10 mg / L, 20 mg / L, and 30 mg / L, respectively, and the rest are the same as in Example 33.

[0041] Comparative Examples 10 and 11 A method for growing Chlorella, referring to Example 33, wherein the addition concentrations in Example 33 are replaced with 0.5 mg / L and 40 mg / L, respectively, and the remainder is the same as in Example 33.

[0042] Example 37 A method for promoting the growth of Chlorella using pharmaceutical products. Referring to Example 31, the 20 mg / L norfloxacin added in Example 31 is replaced with a 2.5 mg / L mixed preparation consisting of four pharmaceutical products. The mixed preparation includes: 2.5 mg / L dexamethasone, 2.5 mg / L norfloxacin, 2.5 mg / L sulfamethoxazole, and 2.5 mg / L indomethacin. The remaining ingredients are the same as in Example 31.

[0043] Example 38 A method for promoting the growth of Chlorella using a pharmaceutical product, referring to Example 31, wherein the 20 0.1 mg / L norfloxacin was replaced with a mixed preparation of 16 pharmaceutical products at 0.1 mg / L, comprising: 0.1 mg / L prednisone and 0.1 mg / L dexamethasone (steroid drugs); 0.1 mg / L norfloxacin, 0.1 mg / L enoxacin, 0.1 mg / L fleroxacin, 0.1 mg / L ofloxacin, 0.1 mg / L ciprofloxacin and 0.1 mg / L lomefloxacin (fluoroquinolone antibiotics); 0.1 mg / L sulfamethazine, 0.1 mg / L sulfamethoxazole, 0.1 mg / L sulfisoxazole, 0.1 mg / L sulfacetamide and 0.1 mg / L sulfadiazine (sulfonamide antibiotics); 0.1 mg / L diclofenac, 0.1 mg / L ibuprofen and 0.1 mg / L indomethacin (non-steroidal anti-inflammatory drugs). The rest is with reference to Example 31.

[0044] Comparative Example 12 A method for growing Chlorella, referring to Example 31, wherein the 20 mg / L norfloxacin added in Example 31 is replaced with 0.01 mg / L of a mixed preparation of 16 pharmaceutical products, the mixed preparation comprising: 0.01 mg / L prednisone and 0.01 mg / L dexamethasone (steroid drugs); 0.01 mg / L norfloxacin, 0.01 mg / L enoxacin, 0.01 mg / L fleroxacin, 0.01 mg / L ofloxacin, 0.01 mg / L ciprofloxacin, and 0.01 mg / L dapoxetine. / L lomefloxacin (fluoroquinolone antibiotic); 0.01mg / L sulfamethazine, 0.01mg / L sulfamethoxazole, 0.01mg / L sulfisoxazole, 0.01mg / L sulfacetamide and 0.01mg / L sulfadiazine (sulfonamide antibiotics); 0.01mg / L diclofenac, 0.01mg / L ibuprofen and 0.01mg / L indomethacin (non-steroidal anti-inflammatory drugs). For the rest, refer to Example 31.

[0045] Result description: 1. Examples 1 to 4 studied the effects of adding different types of pharmaceutical products (steroids, fluoroquinolone antibiotics, sulfonamide antibiotics, or nonsteroidal anti-inflammatory drugs) on the growth of Chlorella vulgaris. Comparative Example 1 was a control group in which no pharmaceutical product was added.

[0046] Calculate the biomass on the eighth day based on the OD-dry weight curve: Chlorella OD 680 Adjust to 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, centrifuge and discard the supernatant, freeze-dry the precipitate, weigh it after freeze-drying, make a standard curve, and use the OD on the eighth day into the standard curve to calculate the biomass of Chlorella.

[0047] In Examples 1 to 4 and Comparative Example 1, OD 680 The curve of change over time is as follows Figure 1 As shown, the biomass results on the eighth day are as follows Figure 2 As shown, the photos of each group on the eighth day are as follows Figure 3 shown.

[0048] Depend on Figure 1 Middle OD 680 The curve of change over time, Figure 2 Biomass results and Figure 3 The darkening of the colors in the photos proves that compared with Comparative Example 1 in which no pharmaceutical product was added, the addition of low concentrations of steroids, fluoroquinolone antibiotics, sulfonamide antibiotics, and non-steroidal anti-inflammatory drugs in Examples 1 to 4 respectively had a significant promoting effect on the growth of Chlorella. These pharmaceutical products may promote the growth and biomass accumulation of Chlorella through various mechanisms (such as metabolic pathway regulation, antioxidant capacity enhancement, competitive microbial inhibition, etc.).

[0049] 2. Examples 5 to 20 studied the effects of adding different types of pharmaceutical products on the growth of Chlorella vulgaris. Comparative Example 2 was used as the control group without adding pharmaceutical products. 680 The curve of change over time is as follows Figure 4 As shown, the OD values of norfloxacin, enoxacin, fleroxacin, ofloxacin, ciprofloxacin, and lomefloxacin in the fluoroquinolone antibiotics were 680 The curve of change over time is as follows Figure 5 As shown, the OD values of sulfamethazine, sulfamethoxazole, sulfisoxazole, sulfacetamide, and sulfadiazine in the sulfonamide antibiotics were 680 The curve of change over time is as follows Figure 6 As shown, the OD values of diclofenac, ibuprofen and indomethacin in NSAIDs were 680 The curve of change over time is as follows Figure 7 shown.

[0050] Depend on Figures 4 to 7 The results show that compared with Comparative Example 1 in which no pharmaceutical product was added, the addition of different types of steroid drugs, different types of fluoroquinolone antibiotics, different types of sulfonamide antibiotics, and different types of non-steroidal anti-inflammatory drugs in Examples 5 to 20 respectively affects the growth of Chlorella, indicating that different types of pharmaceutical products can have a good growth-promoting effect on Chlorella.

[0051] 3. Examples 21 to 36 studied the effect of adding different concentrations of pharmaceutical products on the growth of Chlorella vulgaris. Comparative Example 3 did not add pharmaceutical products, and Comparative Examples 4 to 11 added pharmaceutical products at concentrations of 0.5 mg / L or 40 mg / L, all of which were control groups. On the eighth day, the OD value of dexamethasone was 0.680 The concentration change curve is as follows Figure 8 As shown, the OD of sulfisoxazole was added 680 The concentration change curve is as follows Figure 9 As shown, the OD of norfloxacin was added 680 The concentration change curve is as follows Figure 10 As shown, the OD of indomethacin was added 680 The concentration change curve is as follows Figure 11 shown.

[0052] Depend on Figures 8 to 11 The results show that compared with Comparative Example 3 in which no pharmaceutical product was added and Comparative Examples 4 to 11 in which the pharmaceutical product was added at a concentration of 0.5 mg / L or 40 mg / L, the addition of 5 to 30 mg / L of dexamethasone, sulfisoxazole, norfloxacin, and indomethacin in Examples 21 to 36, respectively, can promote the growth of Chlorella; and when the concentration of the pharmaceutical product is 20 mg / L, the best promoting effect on the growth of Chlorella can be achieved.

[0053] 4. Examples 37, 38 and Comparative Example 12 studied the effects of adding mixed preparations composed of different pharmaceutical products on the growth of Chlorella vulgaris, and compared them with Comparative Example 3 where no pharmaceutical product was added and Example 31 where the pharmaceutical product added was 20 mg / L norfloxacin. In Examples 31, 37 and 38, and Comparative Examples 3 and 12, OD 680 The curve of change over time is as follows Figure 12 shown.

[0054] Depend on Figure 12 The results show that: pharmaceutical product mixtures will affect the growth of Chlorella, and mixtures containing different pharmaceutical pollutants can significantly promote the growth of Chlorella at lower concentrations through synergistic effects.

[0055] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A method for promoting the growth of microalgae using a pharmaceutical product, characterized in that: The method comprises the following steps: activating microalgae on a solid plate, inoculating the activated microalgae into a liquid culture medium, adding pharmaceutical products at the early stage of microalgae growth, placing the microalgae in a constant temperature shaker, and culturing the microalgae under light.

2. The method for promoting microalgae growth using pharmaceutical products according to claim 1, characterized in that: The added concentration of the pharmaceutical product is 0.1~30 mg / L.

3. The method for promoting microalgae growth using pharmaceutical products according to claim 1 or 2, characterized in that: The initial OD of the microalgae 680 The luminance is 0.1-0.3, the shaking speed is 80-150 rpm, the illumination intensity is 1000-3000 lux, and the culture temperature is 25-30°C.

4. The method for promoting microalgae growth using pharmaceutical products according to claim 1, characterized in that: The pharmaceutical product is one or more of steroids, fluoroquinolone antibiotics, sulfonamide antibiotics or non-steroidal anti-inflammatory drugs.

5. The method for promoting microalgae growth using pharmaceutical products according to claim 4, characterized in that: The steroid drugs are prednisone and / or dexamethasone.

6. The method for promoting microalgae growth using pharmaceutical products according to claim 4, characterized in that: The fluoroquinolone antibiotics are one or more of norfloxacin, enoxacin, fleroxacin, ofloxacin, ciprofloxacin or lomefloxacin.

7. The method for promoting microalgae growth using pharmaceutical products according to claim 4, characterized in that: The sulfonamide antibiotic is one or more of sulfamethazine, sulfamethoxazole, sulfisoxazole, sulfacetamide or sulfadiazine.

8. The method for promoting microalgae growth using pharmaceutical products according to claim 4, characterized in that: The nonsteroidal anti-inflammatory drug is one or more of diclofenac, ibuprofen or indomethacin.

9. The method for promoting the growth of microalgae using pharmaceutical products according to claim 1, characterized in that: The microalgae is Chlorella vulgaris, the solid plate is a BG11 solid plate, and the culture medium is a BG11 culture medium.

10. The method for promoting microalgae growth using pharmaceutical products according to claim 9, characterized in that: The activation comprises the following steps: using an inoculation loop to pick up the preserved Chlorella liquid, streaking and culturing on a BG11 solid plate; picking a single algae colony grown on the plate, inoculating it into a liquid culture medium BG11 medium for growth, and expanding the culture after the algae grows to a dark green color. The expanded Chlorella is activated Chlorella.

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