Anti-aging enhanced PDRN composite composition and preparation method thereof

Abstract

The invention belongs to the technical field of nucleotide compositions, and relates to an anti-aging enhanced PDRN compound composition and a preparation method thereof, and the preparation method comprises the following steps: preparing a high-stability PDRN compound; preparing an anti-aging enhanced liquid crystal oil phase carrier; and assembling the PDRN water phase solution and the liquid crystal oil phase solution. The preparation method comprises the following steps: mixing glyceryl monooleate, cetyl-PG hydroxyethyl palmitamide and behenyl alcohol, heating, cooling, adding capryloyl salicylic acid and a beta-glucan-pterostilbene solution, uniformly mixing, cooling at a constant speed, preliminarily forming a lamellar liquid crystal oil phase in the solution, and quenching to shape a liquid crystal structure, so as to obtain the liquid crystal composition. The prepared liquid crystal oil phase enables a subsequent water-in-oil system to have high stability, cetyl-PG hydroxyethyl palmitamide and capryloyl salicylic acid can synergistically increase the transdermal rate of the water-in-oil system, and pterostilbene and beta-glucan can enhance the slow release stability of the system and can synergistically improve the anti-aging ability of the PDRN composite composition to the skin.

Description

Technical Field

[0001] The present invention belongs to the technical field of nucleotide compositions, and in particular relates to an anti-aging enhanced PDRN composite composition and a preparation method thereof. Background Art

[0002] Polydeoxyribonucleotide (PDRN) is a specific DNA fragment extracted from salmon germ cells or salmon sperm vesicles. It has a 98% similarity with human DNA bases and can generate nucleotides and nucleosides that accelerate DNA synthesis, improve the proliferation and growth of fibroblasts, promote skin tissue regeneration, inhibit the expression of inflammatory cytokines and apoptosis proteins, and have anti-inflammatory and resistance to UV damage. However, if the molecular weight of PDRN is too small, it will be difficult to effectively bind to the A2A receptor, thereby reducing its anti-aging effect; if the molecular weight of PDRN is too large and the polarity is strong, it will be difficult to pass through the skin barrier and enrich in the anti-aging target areas of the skin, thereby also reducing its anti-aging effect.

[0003] In order to fully exert the efficacy of PDRN, the existing technology usually uses microneedle injection to inject it into the subcutaneous tissue and prepare it into nanoliposomes so that it can penetrate into the subcutaneous tissue. The microneedle injection method causes certain damage to the skin, and the skin may cause discomfort after injection. In addition, microneedle injection requires professional institutions to operate and has limitations. For the preparation of PDRN into nanoliposomes, the existing technology often uses a conventional oil phase system to directly coat the aqueous phase PDRN. The resulting oil-in-water system has poor stability, and the activity of PDRN is easily lost during storage. Summary of the Invention

[0004] In order to solve the above technical defects, the present invention has developed an anti-aging enhanced PDRN composite composition and its preparation method. The PDRN is first composite-modified and an anti-aging enhanced liquid crystal oil phase carrier is prepared, and then an oil-in-water system is constructed, which can greatly enhance the stability and sustained-release performance of the system and give full play to the anti-aging and skin repair effects of the system.

[0005] A method for preparing an anti-aging enhanced PDRN composite composition comprises the following steps: S1: Preparation of highly stable PDRN compounds In parts by weight, 1-1.2 parts of PDRN and hydroxyethylpiperazineethanesulfonic acid buffer are compounded into a PDRN mother solution, and then 0.8-1 parts of silk fibroin are added, followed by adding 0.08-0.1 parts of trimethylpentanediol / adipic acid / glycerol cross-linked polymer and 0.2-0.25 parts of PPG-24-glycerol polyether-24, ultrasonic treatment is performed, and filtering and cleaning are performed to obtain a highly stable PDRN compound; S2: Preparation of anti-aging enhanced liquid crystal oil phase carrier In parts by weight, 1-1.2 parts of β-glucan, 0.2-0.3 parts of lecithin, and 0.3-0.5 parts of pterostilbene are mixed and filtered in sequence to prepare a β-glucan-pterostilbene solution; 10-12 parts of glyceryl monooleate, 3.5-4 parts of cetyl-PG hydroxyethyl palmitamide, and 2-3 parts of behenyl alcohol are mixed and heated and then cooled; 0.8-1 parts of capryloyl salicylic acid and the β-glucan-pterostilbene solution are added; the temperature is uniformly lowered and then quenched to obtain a liquid crystal oil phase solution; S3: Assembly of PDRN aqueous solution and liquid crystal oil solution In parts by weight, 0.08-0.1 parts of a highly stable PDRN compound is mixed with 10-15 parts of purified water to prepare an aqueous phase solution, which is slowly added dropwise to 50-60 parts of a liquid crystal oil phase solution to obtain an anti-aging enhanced PDRN composite composition.

[0006] Furthermore, step S1 of preparing a highly stable PDRN compound specifically comprises the following steps: S1.1: Place 1-1.2 parts of PDRN in a glass reactor, add 10-12 parts of hydroxyethylpiperazineethanesulfonic acid buffer (pH 7.4), and stir at 200-250 rpm for 20-25 minutes to prepare a PDRN stock solution. S1.2: Add 0.8-1 parts of fibroin to the PDRN mother solution from step S1.1 in 3-4 portions at 5-10°C and 4000-5000 rpm to prepare a precursor solution. S1.3: Take the precursor solution prepared in step S1.2, add 0.08-0.1 parts of trimethylpentanediol / adipic acid / glycerol cross-linked polymer and 0.2-0.25 parts of PPG-24-glycerol polyether-24, transfer to an ultrasonic cell disruptor, and ultrasonically treat at a frequency of 35-40 kHz for 12-15 minutes under a nitrogen atmosphere. After standing for 20-30 minutes, filter and take the solid matter. Wash it with deionized water to obtain a highly stable PDRN compound.

[0007] Furthermore, step S2, the preparation of the anti-aging enhanced liquid crystal oil phase carrier, specifically comprises the following steps: S2.1: Dissolve 1-1.2 parts of β-glucan in 15-20 parts of deionized water at 60-65°C, add 0.2-0.3 parts of lecithin, and sonicate at 25-30 kHz for 15-20 minutes. Then, add 0.3-0.5 parts of pterostilbene, and magnetically stir at 60-65°C and 500-600 rpm for 30-35 minutes. Filter through a microporous filter membrane to obtain a β-glucan-pterostilbene solution. S2.2: Add 10-12 parts of glyceryl monooleate, 3.5-4 parts of cetyl-PG hydroxyethyl palmitamide, and 2-3 parts of behenyl alcohol to a vacuum emulsification reactor, and stir at 70-75°C and 200-250 rpm for 25-30 minutes. Then cool to 40-45°C, add 0.8-1 part of capryloyl salicylic acid and the β-glucan-pterostilbene solution prepared in step S2.1, and stir at a constant temperature of 150-200 rpm for 30-40 minutes. After uniformly cooling to 30-35°C, inject cold nitrogen gas at 5°C and quench to 8-10°C. Keep warm for 10-15 minutes to obtain a liquid crystal oil phase solution.

[0008] Furthermore, step S3 of assembling the PDRN aqueous phase solution and the liquid crystal oil phase solution specifically includes the following steps: S3.1: Mix 0.08-0.1 parts of the highly stable PDRN compound with 10-15 parts of purified water to obtain an aqueous solution. S3.2: Take 50-60 parts of the liquid crystal oil phase solution and heat it to 37-40°C, then add the aqueous phase solution prepared in step S3.1 dropwise into the liquid crystal oil phase solution within 30-40 minutes to obtain a nanoemulsion, which is the anti-aging enhanced PDRN composite composition.

[0009] Furthermore, the PDRN in step S1.1 has a purity of ≥96% and an average molecular weight of 200-850 KDa. Furthermore, the temperature of the hydroxyethylpiperazineethanesulfonic acid buffer in step S1.1 is 3-4°C.

[0010] Furthermore, the time interval between each addition of silk fibroin in step S1.2 is 4-5 minutes.

[0011] Furthermore, the pore size of the microporous filter membrane in step S2.1 is 0.45-0.5 μm.

[0012] Furthermore, the uniform cooling rate in step S2.2 is 1-1.5°C / min.

[0013] An anti-aging enhanced PDRN composite composition is prepared by the preparation method of the anti-aging enhanced PDRN composite composition.

[0014] The beneficial effects of the present invention are: 1. The present invention first modifies β-glucan with lecithin, then adds pterostilbene and stirs to prepare a β-glucan-pterostilbene solution, and then glyceryl monooleate, cetyl-PG hydroxyethyl palmitamide and behenyl alcohol are mixed and heated, and after cooling, capryloyl salicylic acid and β-glucan-pterostilbene solution are added and mixed evenly, and then the temperature is uniformly cooled to initially form a lamellar liquid crystal oil phase in the solution, which is then quenched to finalize the liquid crystal structure. The prepared liquid crystal oil phase can make the subsequent oil-in-water system have high stability, cetyl-PG hydroxyethyl palmitamide and capryloyl salicylic acid can synergistically increase the transdermal rate of the oil-in-water system, pterostilbene and β-glucan can enhance the sustained-release stability of the system, and cetyl-PG hydroxyethyl palmitamide, capryloyl salicylic acid, pterostilbene and β-glucan can also synergistically improve the anti-aging ability of the PDRN composite composition on the skin.

[0015] 2. The present invention adds trimethylpentanediol / adipic acid / glycerol cross-linked polymer and PPG-24-glycerol polyether-24 to the mixed system of polydeoxyribonucleotide and silk fibroin. Under the cross-linked network formed by PPG-24-glycerol polyether-24 and trimethylpentanediol / adipic acid / glycerol cross-linked polymer, polydeoxyribonucleotide and silk fibroin can form a highly stable complex, thereby enhancing the storage stability of PDRN in the subsequent oil-in-water system, ensuring the activity of PDRN when entering the dermis, and improving the repair effect of PDRN on the skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 Flow chart of the preparation method of the anti-aging enhanced PDRN composite composition adopted in the embodiments of the present invention. DETAILED DESCRIPTION

[0017] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0018] It should be noted that, unless otherwise specified, all parts in the following examples represent parts by weight.

[0019] Example 1 A method for preparing an anti-aging enhanced PDRN composite composition, such as Figure 1 As shown, the specific steps include: S1: Preparation of highly stable PDRN compounds S1.1: Place 1 part of PDRN (96% purity, 200 kDa average molecular weight) in a glass reactor. Add 10 parts of hydroxyethylpiperazineethanesulfonic acid buffer (pH 7.4, 3°C) and stir at 200 rpm for 20 minutes to prepare a PDRN stock solution. S1.2: Under high-speed stirring conditions of 4000 rpm and 5°C, add 0.8 parts of fibroin to the PDRN mother solution of step S1.1 in three portions, with an interval of 4 minutes between each addition of fibroin, to prepare a precursor solution; S1.3: Take the precursor solution prepared in step S1.2, add 0.08 parts of trimethylpentanediol / adipic acid / glycerol cross-linked polymer and 0.2 parts of PPG-24-glycerol polyether-24, transfer to an ultrasonic cell disruptor, and ultrasonically treat at a frequency of 35 kHz for 12 minutes under a nitrogen atmosphere. After standing for 20 minutes, filter and take the solid matter. Wash it with deionized water to obtain a highly stable PDRN compound.

[0020] S2: Preparation of anti-aging enhanced liquid crystal oil phase carrier S2.1: Dissolve 1 part β-glucan in 15 parts deionized water at 60°C, add 0.2 parts lecithin, and sonicate at 25 kHz for 15 minutes. Then, add 0.3 parts pterostilbene. Magnetic stirring is performed at 60°C and 500 rpm for 30 minutes. Filter through a microporous filter membrane with a pore size of 0.45 μm to obtain a β-glucan-pterostilbene solution. S2.2: Add 10 parts of monoolein, 3.5 parts of cetyl-PG hydroxyethyl palmitamide and 2 parts of behenyl alcohol to a vacuum emulsification reactor, stir at 70°C and 200 rpm for 25 minutes, then cool to 40°C, add 0.8 parts of capryloyl salicylic acid and the β-glucan-pterostilbene solution prepared in step S2.1, stir at a constant temperature of 150 rpm for 30 minutes, cool uniformly to 30°C at a cooling rate of 1°C / min, inject cold nitrogen at 5°C and quench to 8°C, and keep warm for 10 minutes to obtain a liquid crystal oil phase solution.

[0021] S3: Assembly of PDRN aqueous solution and liquid crystal oil solution S3.1: Mix 0.08 parts of the highly stable PDRN compound with 10 parts of purified water to obtain an aqueous solution. S3.2: Take 50 parts of the liquid crystal oil phase solution and heat it to 37°C, then add the aqueous phase solution prepared in step S3.1 dropwise into the liquid crystal oil phase solution within 30 minutes to obtain a nanoemulsion, which is the anti-aging enhanced PDRN composite composition.

[0022] Example 2 A method for preparing an anti-aging enhanced PDRN composite composition, such as Figure 1 As shown, the specific steps include: S1: Preparation of highly stable PDRN compounds S1.1: Place 1.2 parts of PDRN (96% purity, 200 kDa average molecular weight) in a glass reactor. Add 12 parts of hydroxyethylpiperazineethanesulfonic acid buffer (pH 7.4, 3°C) and stir at 200 rpm for 20 minutes to prepare a PDRN stock solution. S1.2: Under high-speed stirring conditions of 4000 rpm and 5°C, add 1 part of fibroin to the PDRN mother solution of step S1.1 in three portions, with an interval of 4 minutes between each addition of fibroin, to prepare a precursor solution; S1.3: Take the precursor solution prepared in step S1.2, add 0.1 parts of trimethylpentanediol / adipic acid / glycerol cross-linked polymer and 0.25 parts of PPG-24-glycerol polyether-24, transfer to an ultrasonic cell disruptor, and ultrasonically treat at a frequency of 35 kHz for 12 minutes under a nitrogen atmosphere. After standing for 20 minutes, filter and take the solid matter. Wash it with deionized water to obtain a highly stable PDRN compound.

[0023] S2: Preparation of anti-aging enhanced liquid crystal oil phase carrier S2.1: Dissolve 1.2 parts of β-glucan in 20 parts of deionized water at 60°C, add 0.3 parts of lecithin, and sonicate at 25 kHz for 15 minutes. Then, add 0.5 parts of pterostilbene. Magnetic stirring is performed at 60°C and 500 rpm for 30 minutes. The mixture is filtered through a microporous filter membrane with a pore size of 0.45 μm to obtain a β-glucan-pterostilbene solution. S2.2: Add 12 parts of monoolein, 4 parts of cetyl-PG hydroxyethyl palmitamide and 3 parts of behenyl alcohol to a vacuum emulsification reactor, stir at 70°C and 200 rpm for 25 minutes, then cool to 40°C, add 1 part of capryloyl salicylic acid and the β-glucan-pterostilbene solution prepared in step S2.1, stir at a constant temperature of 150 rpm for 30 minutes, cool uniformly to 30°C at a cooling rate of 1°C / min, inject cold nitrogen at 5°C and quench to 8°C, and keep warm for 10 minutes to obtain a liquid crystal oil phase solution.

[0024] S3: Assembly of PDRN aqueous solution and liquid crystal oil solution S3.1: Mix 0.1 parts of the highly stable PDRN compound with 15 parts of purified water to obtain an aqueous solution. S3.2: Take 60 parts of the liquid crystal oil phase solution and heat it to 37°C, then add the aqueous phase solution prepared in step S3.1 dropwise into the liquid crystal oil phase solution within 30 minutes to obtain a nanoemulsion, which is the anti-aging enhanced PDRN composite composition.

[0025] Example 3 A method for preparing an anti-aging enhanced PDRN composite composition, such as Figure 1 As shown, the specific steps include: S1: Preparation of highly stable PDRN compounds S1.1: Place 1 part of PDRN (99% purity, 850 kDa average molecular weight) in a glass reactor. Add 10 parts of hydroxyethylpiperazineethanesulfonic acid buffer (pH 7.4, 4°C) and stir at 250 rpm for 25 minutes to prepare a PDRN stock solution. S1.2: Under high-speed stirring conditions of 10°C and 5000 rpm, add 0.8 parts of fibroin to the PDRN mother solution in step S1.1 in four portions, with an interval of 5 minutes between each addition of fibroin, to prepare a precursor solution; S1.3: Take the precursor solution prepared in step S1.2, add 0.08 parts of trimethylpentanediol / adipic acid / glycerol cross-linked polymer and 0.2 parts of PPG-24-glycerol polyether-24, transfer to an ultrasonic cell disruptor, ultrasonically treat at a frequency of 40 kHz for 15 minutes under a nitrogen atmosphere, let it stand for 30 minutes, and then filter. Take the solid and wash it with deionized water to obtain a highly stable PDRN compound.

[0026] S2: Preparation of anti-aging enhanced liquid crystal oil phase carrier S2.1: Dissolve 1 part β-glucan in 15 parts deionized water at 65°C, add 0.2 parts lecithin, and sonicate at 30 kHz for 15 minutes. Then, add 0.3 parts pterostilbene. Magnetic stirring is performed at 65°C and 600 rpm for 35 minutes. The mixture is filtered through a microporous filter membrane with a pore size of 0.5 μm to obtain a β-glucan-pterostilbene solution. S2.2: Add 10 parts of monoolein, 3.5 parts of cetyl-PG hydroxyethyl palmitamide and 2 parts of behenyl alcohol to a vacuum emulsification reactor, stir at 75°C and 250 rpm for 30 minutes, then cool to 45°C, add 0.8 parts of capryloyl salicylic acid and the β-glucan-pterostilbene solution prepared in step S2.1, stir at a constant temperature of 200 rpm for 40 minutes, cool uniformly to 35°C at a cooling rate of 1.5°C / min, inject cold nitrogen at 5°C and quench to 10°C, and keep warm for 15 minutes to obtain a liquid crystal oil phase solution.

[0027] S3: Assembly of PDRN aqueous solution and liquid crystal oil solution S3.1: Mix 0.08 parts of the highly stable PDRN compound with 10 parts of purified water to obtain an aqueous solution. S3.2: Take 50 parts of the liquid crystal oil phase solution and heat it to 40°C, then add the aqueous phase solution prepared in step S3.1 dropwise into the liquid crystal oil phase solution within 40 minutes to obtain a nanoemulsion, which is the anti-aging enhanced PDRN composite composition.

[0028] Comparative Example 1 The difference from Example 1 is that Comparative Example 1 adopts a conventional oil-in-water system, and the liquid crystal oil phase solution in S3 is replaced by a conventional oil phase solution (Span 80, isopropyl palmitate and Tween 80 are weighed in a mass ratio of 1:1:1.5, Span 80 and isopropyl palmitate are heated at 80°C and mixed, and after cooling to 37°C, Tween 80 is added, stirred and mixed). The remaining specific implementation methods are the same as those in Example 1.

[0029] Comparative Example 2 The difference from Example 1 is that Comparative Example 2 removes step S1.2, replaces the precursor solution in step S1.3 with PDRN mother liquor to prepare a PDRN compound, and replaces the high-stable PDRN compound in step S3 with a PDRN compound of equal quality. The remaining specific implementation methods are the same as Example 1.

[0030] Experiment 1: The anti-aging enhanced PDRN composite composition prepared in Example 1-3 and the PDRN composite composition prepared in Comparative Example 1-2 were taken as samples and stored at room temperature, respectively. The Zeta potential value and the average particle size of the samples were tested when the storage time was 1, 4, and 8 months, respectively. Three parallel experiments were performed and the average value was taken. The results are shown in Table 1.

[0031] Table 1: Zeta potential values and average particle size of samples after storage for different time periods

[0032] It is known that the higher the Zeta potential value, the better the system stability, the smaller the increase in particle size during storage, and the better the system stability. It can be seen from the data of Examples 1-3 and Comparative Examples 1-2 in Table 1 that the anti-aging enhanced PDRN composite composition prepared in Examples 1-3 has high stability. At the same time, the stability of Comparative Example 1-2 is lower than that of Example 1-3. It can be proved that preparing a liquid crystal oil phase solution as the oil phase for oil-in-water can improve the stability of the obtained anti-aging enhanced PDRN composite composition, and the composite modification of PDRN using silk core protein can also improve the stability of the anti-aging enhanced PDRN composite composition.

[0033] Experiment 2: Take the anti-aging enhanced PDRN composite composition prepared in Example 1-3 and the PDRN composite composition prepared in Comparative Example 1-2, and mix them evenly with physiological saline at a mass ratio of 1:10 to prepare test samples for use. Depilation was performed on the same area on the back of 10 SD rats, and the skin moisture content (skin moisture meter), skin elasticity (skin elasticity meter), and wrinkle depth (skin surface three-dimensional scanner) in the depilated area were tested. The average value was taken and recorded as the initial value. Then, the test sample was applied twice a day, 2 mL each time, for 30 consecutive days. The skin moisture content, skin elasticity and wrinkle depth were tested again, and the average value was recorded as the final value. The change value = final value - initial value. The results are shown in Table 2.

[0034] Table 2: Anti-aging effects of test samples on skin

[0035] It can be seen from the data of Examples 1-3 and Comparative Examples 1-2 in Table 2 that the anti-aging enhanced PDRN composite composition prepared in Examples 1-3 has excellent anti-aging effect on the skin, while the anti-aging effect of Comparative Example 1-2 is significantly decreased compared with that of Examples 1-3. This proves that preparing a liquid crystal oil phase solution as the oil phase for oil-in-water can enhance the anti-aging effect of the prepared PDRN composite composition, and that composite modification of PDRN using silk fibroin can also enhance the anti-aging effect of the PDRN composite composition.

[0036] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by one of ordinary skill in the art without departing from the spirit and technical principles disclosed herein are intended to be covered by the claims of the present invention.

Claims

1. A method for preparing an anti-aging enhanced PDRN composite composition, characterized in that, The following steps are involved: S1: Preparation of highly stable PDRN compounds In parts by weight, 1-1.2 parts of PDRN and hydroxyethylpiperazineethanesulfonic acid buffer are compounded into a PDRN mother solution, and then 0.8-1 parts of silk fibroin are added, followed by adding 0.08-0.1 parts of trimethylpentanediol / adipic acid / glycerol cross-linked polymer and 0.2-0.25 parts of PPG-24-glycerol polyether-24, ultrasonic treatment is performed, and filtering and cleaning are performed to obtain a highly stable PDRN compound; S2: Preparation of anti-aging enhanced liquid crystal oil phase carrier In parts by weight, 1-1.2 parts of β-glucan, 0.2-0.3 parts of lecithin, and 0.3-0.5 parts of pterostilbene are mixed and filtered in sequence to prepare a β-glucan-pterostilbene solution; 10-12 parts of glyceryl monooleate, 3.5-4 parts of cetyl-PG hydroxyethyl palmitamide, and 2-3 parts of behenyl alcohol are mixed and heated and then cooled; 0.8-1 parts of capryloyl salicylic acid and the β-glucan-pterostilbene solution are added; the temperature is uniformly lowered and then quenched to obtain a liquid crystal oil phase solution; S3: Assembly of PDRN aqueous solution and liquid crystal oil solution In parts by weight, 0.08-0.1 parts of a highly stable PDRN compound is mixed with 10-15 parts of purified water to prepare an aqueous phase solution, which is slowly added dropwise to 50-60 parts of a liquid crystal oil phase solution to obtain an anti-aging enhanced PDRN composite composition.

2. the preparation method of a kind of anti-aging enhanced PDRN composite composition according to claim 1, is characterized in that, Step S1: Preparation of a highly stable PDRN compound, specifically comprising the following steps: S1.1: Place 1-1.2 parts of PDRN in a glass reactor, add 10-12 parts of hydroxyethylpiperazineethanesulfonic acid buffer (pH 7.4), and stir at 200-250 rpm for 20-25 minutes to prepare a PDRN stock solution. S1.2: Add 0.8-1 parts of fibroin to the PDRN mother solution from step S1.1 in 3-4 portions at 5-10°C and 4000-5000 rpm to prepare a precursor solution. S1.3: Take the precursor solution prepared in step S1.2, add 0.08-0.1 parts of trimethylpentanediol / adipic acid / glycerol cross-linked polymer and 0.2-0.25 parts of PPG-24-glycerol polyether-24, transfer to an ultrasonic cell disruptor, and ultrasonically treat at a frequency of 35-40 kHz for 12-15 minutes under a nitrogen atmosphere. After standing for 20-30 minutes, filter and take the solid matter. Wash it with deionized water to obtain a highly stable PDRN compound.

3. the preparation method of a kind of anti-aging enhanced PDRN composite composition according to claim 2, is characterized in that, Step S2 is the preparation of the anti-aging enhanced liquid crystal oil phase carrier, which specifically includes the following steps: S2.1: Dissolve 1-1.2 parts of β-glucan in 15-20 parts of deionized water at 60-65°C, add 0.2-0.3 parts of lecithin, and sonicate at 25-30 kHz for 15-20 minutes. Then, add 0.3-0.5 parts of pterostilbene, and magnetically stir at 60-65°C and 500-600 rpm for 30-35 minutes. Filter through a microporous filter membrane to obtain a β-glucan-pterostilbene solution. S2.2: Add 10-12 parts of glyceryl monooleate, 3.5-4 parts of cetyl-PG hydroxyethyl palmitamide, and 2-3 parts of behenyl alcohol to a vacuum emulsification reactor, and stir at 70-75°C and 200-250 rpm for 25-30 minutes. Then cool to 40-45°C, add 0.8-1 part of capryloyl salicylic acid and the β-glucan-pterostilbene solution prepared in step S2.1, and stir at a constant temperature of 150-200 rpm for 30-40 minutes. After uniformly cooling to 30-35°C, inject cold nitrogen gas at 5°C and quench to 8-10°C. Keep warm for 10-15 minutes to obtain a liquid crystal oil phase solution.

4. the preparation method of a kind of anti-aging enhanced PDRN composite composition according to claim 3, is characterized in that, Step S3: assembling the PDRN aqueous phase solution and the liquid crystal oil phase solution, specifically comprising the following steps: S3.1: Mix 0.08-0.1 parts of the highly stable PDRN compound with 10-15 parts of purified water to obtain an aqueous solution. S3.2: Take 50-60 parts of the liquid crystal oil phase solution and heat it to 37-40°C, then add the aqueous phase solution prepared in step S3.1 dropwise into the liquid crystal oil phase solution within 30-40 minutes to obtain a nanoemulsion, which is the anti-aging enhanced PDRN composite composition.

5. the preparation method of a kind of anti-aging enhanced PDRN composite composition according to claim 2, is characterized in that, The PDRN in step S1.1 has a purity of ≥96% and an average molecular weight of 200-850 kDa.

6. the preparation method of a kind of anti-aging enhanced PDRN composite composition according to claim 2, is characterized in that, The temperature of the hydroxyethylpiperazineethanesulfonic acid buffer in step S1.1 is 3-4°C.

7. the preparation method of a kind of anti-aging enhanced PDRN composite composition according to claim 2, is characterized in that, The time interval between each addition of silk fibroin in step S1.2 is 4-5 minutes.

8. The preparation method of a PDRN composite composition having anti-aging enhancement according to claim 3, wherein The pore size of the microporous filter membrane in step S2.1 is 0.45-0.5 μm.

9. the preparation method of a kind of anti-aging enhanced PDRN composite composition according to claim 3, is characterized in that, The uniform cooling rate in step S2.2 is 1-1.5°C / min.

10. An anti-aging enhanced PDRN composite composition, characterized in that, It is prepared by the preparation method of an anti-aging enhanced PDRN composite composition according to any one of claims 1 to 8.

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