Macadamia nut polypeptide-containing toothpaste and preparation method thereof

Abstract

The invention relates to the technical field of oral care products, in particular to macadamia nut polypeptide toothpaste and a preparation method thereof. The toothpaste containing the macadamia nut polypeptide is prepared from the following raw materials in parts by weight: 2 to 3 parts of the macadamia nut polypeptide, 15 to 30 parts of a friction agent, 10 to 75 parts of a humectant, 0.5 to 5 parts of a surfactant, 0.1 to 5 parts of a sweetening agent, 0.5 to 3 parts of a thickening agent and 0.1 to 2 parts of essence, the abrasives are conventional abrasives and natural abrasives; the conventional abrasive comprises at least one of silicon dioxide, calcium bicarbonate and calcium hydrogen phosphate dihydrate; the natural abrasive is macadamia nut shell powder. According to the technical scheme, the technical problem that in the prior art, an oral care product containing macadamia nut polypeptide with good stability and efficacy is lacked can be solved. According to the scheme, oral care products containing macadimia nut polypeptide are deeply researched and developed, so that the market potential of the economic tree species of macadimia nuts is further improved.

Description

Technical Field

[0001] The present invention relates to the technical field of oral care products, and in particular to a toothpaste containing macadamia polypeptide and a preparation method thereof. Background Art

[0002] As a natural bioactive ingredient, the peptides in macadamia kernels are bioactive peptides prepared by enzymatic hydrolysis of macadamia protein. They have many potential functions, including antioxidant, blood pressure lowering, immunomodulatory, and antibacterial properties. Macadamia peptides have the ability to scavenge free radicals, which can protect cells from oxidative damage, thereby delaying the aging process. Macadamia peptides can also regulate the function of the immune system, enhance the body's immunity, and have a positive effect on resisting infection and disease. In addition, macadamia peptides also have antibacterial, anti-inflammatory, and anti-tumor functions. Macadamia peptides are rich in various amino acids and can be used as a nutritional supplement to enhance the body's immunity. Due to their antioxidant and antibacterial properties, macadamia peptides are widely used in the development of health foods, such as health products and functional beverages.

[0003] However, there are currently few reports on the use of macadamia peptides in oral care products. In fact, the antioxidant and antibacterial properties of macadamia peptides are very effective for maintaining oral health, and macadamia peptides have considerable potential application value in the oral care field. However, how to incorporate macadamia peptides into oral care products while ensuring their effectiveness and compatibility with conventional oral care ingredients presents a new challenge in the application of macadamia peptides.

[0004] In summary, with the global rise in health awareness and the continued growth in consumer demand for high-quality health products, macadamia peptides, with their natural, safe, and highly effective bioactive properties, present broad application prospects. Their potential value, particularly in the oral care sector, cannot be underestimated. Therefore, in-depth research and development of oral care products containing macadamia peptides is not only of great scientific significance but also holds enormous market potential. To promote progress in this field, researchers must focus on addressing key issues such as the stability and bioavailability of active ingredients to meet market demand. Summary of the Invention

[0005] The present invention aims to provide a toothpaste containing macadamia polypeptides to solve the technical problem of the lack of stable and effective oral care products containing macadamia polypeptides in the prior art.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A toothpaste containing macadamia polypeptide, wherein the raw materials include, by weight: 2-3 parts of macadamia polypeptide, 15-30 parts of abrasive, 10-75 parts of moisturizer, 0.5-5 parts of surfactant, 0.1-5 parts of sweetener, 0.5-3 parts of thickener, and 0.1-2 parts of flavor;

[0008] The friction agent includes a conventional friction agent and a natural friction agent; the conventional friction agent includes at least one of silicon dioxide, calcium bicarbonate and calcium hydrogen phosphate dihydrate; and the natural friction agent is macadamia nut shell powder.

[0009] Furthermore, the macadamia nut polypeptide is prepared by the following method: removing the oil from the kernel of macadamia nuts through a cold pressing process to obtain fruit meal; soaking the fruit meal in anhydrous ethanol to defatted, and then drying to constant weight; crushing the dried fruit meal to obtain fruit meal powder; boiling the fruit meal powder in boiling water, and after cooling, enzymatically hydrolyzing the fruit meal powder with papain; after the enzymatic hydrolysis is completed, boiling the mixture and then centrifuging to collect the supernatant; drying the supernatant to obtain a powdered macadamia nut polypeptide with a moisture content of less than 5%.

[0010] Furthermore, the method of enzymatic hydrolysis of fruit meal powder with papain is as follows: the fruit meal powder is dispersed in boiling water and kept for 10 minutes, then the boiling water is cooled to 37°C, the pH value is adjusted to 7.0, and papain is added to obtain an enzymatic hydrolysis system; the mass percentage of fruit meal powder in the enzymatic hydrolysis system is 20%, the mass percentage of papain in the fruit meal powder is 0.5%, and the enzymatic hydrolysis is carried out at a constant temperature for 8 hours.

[0011] Furthermore, the water content of the macadamia nut shell powder is ≤5% and the particle size is 20-100 μm.

[0012] Furthermore, the moisturizing agent includes a first moisturizing agent and a second moisturizing agent; the first moisturizing agent is glycerin and / or sorbitol; and the second moisturizing agent is polyethylene glycol.

[0013] Furthermore, the thickener includes at least one of sodium carboxymethyl cellulose, carrageenan, guar gum and xanthan gum.

[0014] Furthermore, the surfactant includes at least one of sodium lauryl sulfate, cocoyl alanine triacetate ammonium, sodium laureth sulfate and cocoyl glutamate triethanolamine.

[0015] Furthermore, the sweetener includes at least one of saccharin sodium, aspartame, stevia, sucralose, and xylitol.

[0016] Furthermore, the raw materials include, by weight: 3 parts of macadamia nut polypeptide, 15 parts of abrasive, 55 parts of moisturizer, 3 parts of surfactant, 1 part of sweetener, 2.5 parts of thickener, and 0.5 part of flavor;

[0017] The mass ratio of conventional friction agent to natural friction agent is 1:2.

[0018] The present technical solution also provides a method for preparing a toothpaste containing macadamia polypeptide, characterized in that macadamia shell powder and water are mixed in a ratio of 5:1 and mixed evenly; after 20-40 minutes, a second moisturizing agent is added and mixed with the moistened macadamia shell powder to obtain a mixture A;

[0019] Dispersing the thickener in the first moisturizer to obtain mixture B; dispersing the macadamia polypeptide in mixture B and allowing it to stand for 20-40 minutes to obtain mixture C;

[0020] Adding a surfactant, a sweetener, and the remaining water to mixture C to obtain mixture D;

[0021] Mix mixture A and mixture D, add conventional friction agent, stir and homogenize in a paste making machine and evacuate;

[0022] Add essence, stir and homogenize, and deaerate to obtain toothpaste.

[0023] The technical principle of this technical solution is:

[0024] The present invention deeply explores the application potential of macadamia nut polypeptide in the field of oral care, and successfully develops a toothpaste containing macadamia nut polypeptide and its preparation method.

[0025] Macadamia polypeptide is a biologically active polypeptide component extracted from macadamia nut protein through an enzymatic hydrolysis process. It has multiple biological activities such as antioxidant properties and is the main functional ingredient of the toothpaste of the present invention. Macadamia nut shell powder, as a natural abrasive, not only has ideal physical properties (such as suitable particle size and hardness), but also can enhance the effect of macadamia nut polypeptide under appropriate processing methods. The microstructure of the shell powder surface helps to adsorb and stabilize the polypeptide component, preventing its degradation during the preparation and storage of toothpaste. In addition, the shell powder itself also has certain antibacterial and antioxidant properties, further enhancing the oral care effect of the toothpaste.

[0026] In addition to macadamia nut peptides and shell powder, the toothpaste formula also incorporates humectants, surfactants, sweeteners, thickeners, and flavors. The selection and proportions of these ingredients have been carefully optimized to ensure the toothpaste's stability, taste, and user experience. Humectants maintain the toothpaste's moisturizing properties, preventing it from drying out during use; surfactants reduce the toothpaste's surface tension, making it easier to spread and cleanse the mouth; and sweeteners and flavors enhance the toothpaste's taste and aroma, improving user acceptance.

[0027] During the preparation process, the present invention employs specific process steps and parameters to ensure the effective utilization and stable dispersion of macadamia nut polypeptides and husk powder. For example, when preparing Mixture A, the ratio of husk powder to water and the mixing time are controlled to ensure that the husk powder is fully infiltrated, allowing for adequate modification of the husk powder by polyethylene glycol. When preparing Mixture C, the dispersion of the macadamia nut polypeptide is controlled to ensure its stable presence in the mixture.

[0028] In summary, this proposal utilizes the natural bioactive components of macadamia nut peptides and the physical properties of macadamia nut shell powder to develop a toothpaste product with significant antioxidant and antibacterial effects through an optimized formula and innovative preparation process.

[0029] The beneficial effects of this technical solution are:

[0030] (1) Enhance oral health: The antioxidant and antibacterial functions of macadamia nut peptides help maintain oral health, reduce the damage of free radicals to oral cells, inhibit the growth of harmful bacteria, thereby protecting oral tissues and preventing oral diseases.

[0031] The antioxidant effect of the toothpaste of the present invention was verified by measuring the DPPH free radical scavenging rate. The experimental results showed that the antioxidant effect of the toothpaste significantly improved with increasing the amount of macadamia nut polypeptide added. Furthermore, compared with the comparative example, the toothpaste of the present invention demonstrated significant advantages in both stability and antioxidant efficacy.

[0032] (2) Improved stability: The stability of the toothpaste was enhanced through a specific preparation process, specifically the use of macadamia nut shell powder as a natural abrasive and polyethylene glycol modification of its surface. This modification not only improved the compatibility of the shell powder with other toothpaste ingredients but also enhanced the storage stability of the toothpaste at both high and room temperatures.

[0033] (3) Optimized user experience: The toothpaste of the present invention has a reasonable formula and good compatibility between ingredients. The prepared toothpaste is uniform and smooth, with a pleasant mouthfeel. The rational selection of moisturizers and thickeners makes the toothpaste easy to squeeze out, with moderate foaming, and a better brushing experience.

[0034] (4) Natural safety: As a natural bioactive ingredient, macadamia peptides are highly safe and biocompatible. Compared with traditional chemically synthesized ingredients, the application of macadamia peptides is more in line with modern consumers' demand for natural and healthy products.

[0035] (5) Fully utilizing natural resources: The present invention utilizes the peptides and shell components of macadamia nuts, not only providing a natural active ingredient for the oral care field, but also achieving the in-depth development and sustainable utilization of macadamia nuts as a natural resource. This helps promote the resource utilization of agricultural waste, reduce environmental pollution, and promote the development of the local economy. Through the implementation of the present invention, the industrial chain of macadamia nuts has been extended, its added value has been increased, and it has brought more economic and social benefits to related industries.

[0036] In summary, the present invention provides a toothpaste containing macadamia polypeptide and a preparation method thereof. The toothpaste has significant antioxidant effect, good stability, good user experience, and natural and safe ingredients, which meets the needs of modern consumers for high-quality oral care products. DETAILED DESCRIPTION

[0037] The present invention will be further described in detail below with reference to the examples, but the embodiments of the present invention are not limited thereto. Unless otherwise specified, the technical means used in the following examples and experimental examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used are all commercially available.

[0038] The following is further described in detail through specific implementation methods:

[0039] Example 1: Preparation of macadamia polypeptide

[0040] The preparation method of macadamia polypeptide used in this scheme refers to the applicant's prior patent "CN115887331B A Panax notoginseng flower essence containing macadamia oil and polypeptide and its preparation method". The specific process is as follows:

[0041] The kernels of macadamia nuts are subjected to conventional cold pressing to extract oil. After the oil is separated, the remaining portion is the meal, which is recycled for the preparation of macadamia nut polypeptides. The meal remaining after the cold pressing process is taken, soaked in anhydrous ethanol for 10 minutes to defatted, and then taken out and dried to constant weight. The dried meal is then crushed and passed through an 80-mesh sieve to obtain meal powder. The meal powder is dispersed in boiling water and kept for 10 minutes, and then the boiling water is cooled to 37°C, the pH value is adjusted to 7.0, and papain is added to obtain an enzymatic hydrolysis system. The mass percentage of meal powder in the enzymatic hydrolysis system is 20%, and the mass percentage of papain (800u / mg, CAS: 9001-73-4) in the meal powder is 0.5%, and the enzymatic hydrolysis is carried out at a constant temperature for 8 hours. After the enzymatic hydrolysis is completed, the system is heated to boiling for 10 minutes. The mixture was then centrifuged at 4000 rpm for 15 minutes, and the supernatant was collected and subjected to reduced pressure evaporation using a rotary evaporator for 5 hours to obtain an extract, which was then freeze-dried to obtain a dry powder of macadamia polypeptide (with a water content of less than 5%). The macadamia polypeptide was used to prepare toothpaste.

[0042] Example 2: Preparation of natural friction agent

[0043] Take fresh macadamia nut shells, dry them to a moisture content of ≤5%, then ultrafinely grind them to a particle size of 20-100μm, and use them as a natural abrasive for toothpaste (macadamia nut shell powder). Macadamia nut shells are rich in natural plant fibers (which can be used as abrasives), and are rich in antioxidants, phenolic compounds and other beneficial ingredients. After drying and grinding, they can be used as a substitute for abrasives in toothpaste. Generally speaking, macadamia nut shells need to be harmlessly treated after the kernels are removed and then discarded. This technical solution uses macadamia nut shells to prepare toothpaste abrasives, which not only helps to reduce the impact of agricultural waste on the environment, but also creates additional economic value.

[0044] Example 3: Toothpaste containing macadamia polypeptide

[0045] (1) Composition

[0046] The toothpaste containing macadamia polypeptide comprises the following components, calculated by weight: 2-3 parts of macadamia polypeptide, 15-30 parts of abrasive, 10-75 parts of moisturizer, 0.5-5 parts of surfactant, 0.1-5 parts of sweetener, 0.5-3 parts of thickener and 0.1-2 parts of flavor.

[0047] The friction agent includes a conventional friction agent and a natural friction agent in a mass ratio of 1:2; the conventional friction agent includes at least one of silicon dioxide, calcium bicarbonate and calcium hydrogen phosphate dihydrate; the natural friction agent is macadamia nut shell powder, which is prepared according to the method of Example 2 above.

[0048] The moisturizing agent comprises at least one of glycerol, sorbitol and polyethylene glycol. The moisturizing agent comprises a first moisturizing agent and a second moisturizing agent in a mass ratio of 45:10, wherein the first moisturizing agent is glycerol and / or sorbitol, and the second moisturizing agent is polyethylene glycol.

[0049] The thickener includes at least one of sodium carboxymethyl cellulose, carrageenan, guar gum and xanthan gum.

[0050] The surfactant includes at least one of sodium lauryl sulfate, cocoyl alanine triacetate ammonium, sodium laureth sulfate and cocoyl glutamate triethanolamine.

[0051] The sweetener includes at least one of saccharin sodium, aspartame, stevia, sucralose, and xylitol.

[0052] Flavors can be selected from conventional flavors used in oral care products, such as lemon flavor, fruit flavor, spearmint flavor, etc.

[0053] (2) Preparation method

[0054] Deionized water was added to the macadamia nut shell powder at a ratio of 5:1 and mixed thoroughly. After approximately 30 minutes (20-40 minutes may be optional), polyethylene glycol (a second moisturizer) was added and thoroughly mixed with the moistened macadamia nut shell powder to obtain a mixture A.

[0055] Disperse the thickener in the first moisturizer and mix well to obtain a mixture B. Disperse the macadamia polypeptide in the mixture B and mix well to obtain a mixture C, which is allowed to stand for about 30 minutes (optionally 20-40 minutes) before use.

[0056] The surfactant, sweetener, and the remaining water were added to the mixture C that had been allowed to stand for 30 minutes, and mixed evenly to obtain a mixture D.

[0057] Mixture A and mixture D were mixed, and then a conventional abrasive was added, and the mixture was stirred and homogenized in a paste making machine for 30 minutes and vacuumed. Finally, flavor was added, and the mixture was stirred and homogenized, and degassed to obtain toothpaste.

[0058] More specifically, the formulations of Examples 1-3 and Comparative Examples 1-3 are shown in Table 1. The formulations of Comparative Examples 4-6 are consistent with that of Example 3, with only the preparation methods being different.

[0059] Table 1: Examples and Comparative Examples (raw materials are expressed in parts by mass, 100 parts in total)

[0060] raw material Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Macadamia Nut Peptides 2 2.5 3 3 3 0 Macadamia Nut Shell Powder 10 10 10 0 10 10 Silicon dioxide 5 5 5 15 5 5 sorbitol 45 45 45 45 55 45 Polyethylene glycol 300 10 10 10 10 - 10 lemon essence 0.5 0.5 0.5 0.5 0.5 0.5 Sodium lauryl sulfate 3 3 3 3 3 3 Xanthan gum 2 2 2 2 2 2 Sodium carboxymethyl cellulose 0.5 0.5 0.5 0.5 0.5 0.5 Sucralose 1 1 1 1 1 1 water margin margin margin margin margin margin

[0061] The preparation methods of the toothpastes of Examples 1-3 refer to the preparation methods described above ("(2) Preparation method").

[0062] Comparative Example 1 does not use macadamia shell powder as a friction agent. Instead, the friction agent is entirely made of silica. The preparation method is as follows: thickeners (xanthan gum and sodium carboxymethyl cellulose) are dispersed in humectants (sorbitol and polyethylene glycol 300) and mixed evenly. Then, macadamia polypeptide is dispersed in the above system and mixed evenly. A surfactant, a sweetener, and the remainder of water are added and mixed evenly. Next, a silica friction agent is added, and the mixture is stirred and homogenized in a paste making machine for 30 minutes and vacuumed. Finally, flavoring is added, stirred and homogenized, and degassed to obtain toothpaste.

[0063] In Comparative Example 2, polyethylene glycol was not used, and its preparation method was specifically as follows:

[0064] Deionized water was added to the macadamia nut shell powder at a ratio of 5:1, mixed evenly, and allowed to stand for about 30 minutes to obtain a mixture A.

[0065] Disperse the thickener in the sorbitol moisturizer and mix thoroughly to obtain Mixture B. Disperse the macadamia polypeptide in Mixture B and mix thoroughly to obtain Mixture C, which is allowed to stand for approximately 30 minutes. Add the surfactant, sweetener, and remaining water to Mixture C, which has been allowed to stand for 30 minutes, and mix thoroughly to obtain Mixture D.

[0066] Mixture A and mixture D were mixed, and then a conventional abrasive was added, and the mixture was stirred and homogenized in a paste making machine for 30 minutes and vacuumed. Finally, flavor was added, and the mixture was stirred and homogenized, and degassed to obtain toothpaste.

[0067] In Comparative Example 3, macadamia polypeptide was not used. The preparation method was essentially the same as in Example 3, except that the addition of macadamia polypeptide was omitted. After the thickener was dispersed in the first humectant, the surfactant, sweetener, and balance water were added directly. The operation method was similar to that in Example 3.

[0068] Comparative Example 4

[0069] The formula of this comparative example is the same as that of Example 3, except for the preparation method: the macadamia nut shell powder is not soaked with water or mixed with polyethylene glycol. Instead, the macadamia nut shell powder and silicon dioxide are directly added at the same process position. The details are as follows:

[0070] Disperse the thickeners (xanthan gum and sodium carboxymethylcellulose) in the humectants (sorbitol and polyethylene glycol 300) and mix well. Then, disperse the macadamia polypeptide in the above system, mix well, and let it sit for about 30 minutes. Add a surfactant, sweetener, and the remaining water and mix well. Next, add silica abrasives and macadamia shell powder abrasives, stir and homogenize in a paste making machine for 30 minutes and evacuate. Finally, add the flavoring, stir and homogenize, and degas to obtain the toothpaste.

[0071] Comparative Example 5

[0072] The formula of this comparative example is the same as that of Example 3, except that the preparation method is as follows: the macadamia nut shell powder is not soaked with water, but is directly mixed with polyethylene glycol.

[0073] Add polyethylene glycol (second moisturizer) to macadamia shell powder and mix thoroughly to obtain mixture A. Disperse the thickener in the first moisturizer and mix well to obtain mixture B. Disperse the macadamia polypeptide in mixture B and mix well to obtain mixture C, which is allowed to stand for about 30 minutes. Add a surfactant, sweetener, and the remaining water to mixture C, which has been allowed to stand for 30 minutes, and mix well to obtain mixture D. Mixture A and mixture D are mixed, and then a conventional abrasive is added. Stir and homogenize in a paste making machine for 30 minutes and vacuum. Finally, add flavoring, stir and homogenize, deaerate, and obtain toothpaste.

[0074] Comparative Example 6

[0075] The formula of this comparative example is the same as that of Example 3, except that the preparation method is as follows: the macadamia polypeptide is processed differently and is not directly added to the mixture of the thickener and the first wetting agent. Specifically: deionized water is added to the macadamia shell powder at a ratio of 5:1 and mixed evenly. After about 30 minutes, polyethylene glycol (the second wetting agent) is added and thoroughly mixed with the moistened macadamia shell powder to obtain mixture A. The thickener is dispersed in the first wetting agent and mixed evenly to obtain mixture B. A surfactant, a sweetener and the remainder of water are added to mixture B and mixed evenly to obtain mixture C. Finally, macadamia polypeptide is added and mixed evenly to obtain mixture D. Mixture A and mixture D are mixed, and then a conventional friction agent is added, stirred and homogenized in a paste maker for 30 minutes and vacuumed. Finally, flavor is added, stirred and homogenized, degassed, and toothpaste is obtained.

[0076] Experimental Example 1: Antioxidant Effect of Toothpaste

[0077] The antioxidant effect of toothpaste was studied by measuring the DPPH free radical scavenging rate. The experimental method can be found in the literature (Fu Jiarong, Preparation process and antioxidant activity of macadamia polypeptides with different molecular weights, Food Industry Science and Technology, October 2023). Prepare a 0.2 mmol / L DPPH solution with anhydrous ethanol and store in the dark. Accurately measure 2 mL of the sample solution in a test tube, add 2 mL of DPPH solution and shake well. React at room temperature in the dark for 30 minutes. Measure the absorbance at a wavelength of 517 nm as A1. Replace the DPPH solution with 2 mL of anhydrous ethanol and measure its absorbance as A2. Replace the sample solution with 2 mL of distilled water and measure its absorbance as A0. The DPPH free radical scavenging rate is calculated as: (1-(A1-A2) / A0)×100%.

[0078] The sample solution was prepared as follows: Approximately 2.0 g of the toothpaste samples from the Examples and Comparative Examples were placed in a 50 mL centrifuge tube. 10 mL of pre-chilled phosphate buffered saline (PBS, pH 7.4) was added and vortexed for 2 minutes to fully disperse the sample. PBS maintains the pH stability of the solution while penetrating into the paste matrix, disrupting the interaction between the surfactant and the protein / peptide. The centrifuge tube was placed in an ice-water bath and sonicated (200 W power, 40 kHz frequency) for 10 minutes (5 seconds on, 5 seconds off). The cavitation effect of ultrasound disrupts the micelle structure in the paste, releasing the encapsulated polypeptides, while the low temperature prevents polypeptide degradation. The sample was centrifuged at 12,000 rpm for 20 minutes (4°C). The supernatant was transferred to a new centrifuge tube, and the precipitate (primarily containing abrasives, etc.) was discarded to separate the soluble components (including polypeptides) from the insoluble particles. The supernatant obtained through the above preparation process is the sample solution for DPPH free radical scavenging rate determination.

[0079] The test results of Examples 1-3 and Comparative Examples 1-6 are detailed in Table 2. It can be seen from the experimental data that the free radical scavenging rates of Examples 1-3 increase with the addition of macadamia polypeptide. In Comparative Example 3 (Comparative Example 3 is significantly different from Example 3, t-test, P < 0.05), macadamia polypeptide was not used, and its DPPH free radical scavenging rate was very low, and basically no antioxidant effect existed. In Comparative Example 1 (Comparative Example 1 is significantly different from Example 3, t-test, P < 0.05), macadamia nut shell powder was not used as a natural abrasive for toothpaste. Compared with Example 3, the DPPH free radical scavenging function of toothpaste decreased, which shows that the addition of macadamia nut shell powder plays a positive role in enhancing the effect of macadamia nut polypeptide. The inventor analyzed that the macadamia nut shell powder may have a certain protective effect on the polypeptide. In Comparative Example 2 (Comparative Example 2 is significantly different from Example 3, t-test, P < 0.05), macadamia nut shell powder and macadamia nut polypeptide are added at the same time. However, polyethylene glycol is not used to modify the surface of the macadamia nut shell powder (polyethylene glycol is not added to the formula), and the effect of macadamia nut shell powder on the efficacy of macadamia nut polypeptide is reduced. The inventor analyzed that the shell powder needs to be treated and surface modified in order to exert the protective effect of the polypeptide. In Comparative Example 4 (Comparative Example 4 is significantly different from Example 3, t-test, P < 0.05), the macadamia nut shell powder is not soaked in deionized water and modified with polyethylene glycol, and the macadamia nut shell powder and silica are added together at the end of the process. Although polyethylene glycol is added to the formula, the overall free radical scavenging effect of the toothpaste is not ideal. This also illustrates the importance of polyethylene glycol for the surface modification of the shell powder. The shell powder cannot be treated in the same way as ordinary abrasives. Comparative Example 5 did not use deionized water for immersion before polyethylene glycol modification, resulting in a reduced enhancement of the macadamia nut shell powder's efficacy on the macadamia nut polypeptide. The inventors believe this may be due to the unsatisfactory effect of polyethylene glycol modification on the macadamia nut shell powder that had not been immerged in water. Comparative Example 6 (Comparative Example 6 showed a significant difference compared to Example 3, t-test, P < 0.05) incorporated the macadamia nut polypeptide differently from Example 3, resulting in a negative impact on its antioxidant efficacy.

[0080] Table 2: DPPH free radical scavenging rate (%) of Examples and Comparative Examples

[0081] Grouping Repeat 1 Repeat 2 Repeat 3 average value Example 1 58.2 56.7 55.6 56.8±1.3 Example 2 63.8 65.5 67.7 65.7±2.0 Example 3 72.5 75.8 70.4 72.9±2.7 Comparative Example 1 58.3 55.1 58.8 57.4±2.0 Comparative Example 2 63.6 66.3 64.0 64.6±1.5 Comparative Example 3 4.2 3.3 2.1 3.2±1.1 Comparative Example 4 65.7 66.9 64.3 65.6±1.3 Comparative Example 5 69.4 70.1 64.3 67.9±3.1 Comparative Example 6 60.1 62.6 60.8 61.2±1.3

[0082] Experimental Example 2: Stability of toothpaste

[0083] In order to investigate the effect of the addition of macadamia nut polypeptide and shell powder on the stability of the paste, the toothpastes of the embodiment and the comparative example were stored at room temperature and high temperature (45°C), respectively. The appearance of the paste was observed after three months, and the DPPH radical scavenging rate of the sample after three months was tested according to the method of Experimental Example 1.

[0084] Three months later, the results of the observation of the appearance of the paste are shown in Table 3. The toothpaste prepared according to this scheme has good stability. For details, please refer to the experimental results of Examples 1-3. In Comparative Example 1, no nut shell powder was added, and the stability of the paste was not negatively affected, indicating that the addition of nut shell powder is the main factor affecting the stability of the paste. In Comparative Example 2, no polyethylene glycol was added, and the nut shell powder was not modified, resulting in poor paste stability. Although no polypeptide was added in Comparative Example 3, the nut shell powder was modified in the manner of this scheme, so the paste stability was also relatively ideal. Although polyethylene glycol was used in Comparative Example 4, the nut shell powder was not modified in the manner of this scheme, resulting in unsatisfactory paste stability. In Comparative Example 5, the nut shell powder was not soaked before being modified with polyethylene glycol, which had a certain negative impact on the stability of the paste. Comparative Example 6 used a different method of treating the polypeptide, which affected the overall stability of the toothpaste to a certain extent.

[0085] Table 3: Observation results of paste appearance

[0086]

[0087] The above is only an embodiment of the present invention, and the common knowledge such as the specific technical solutions and / or characteristics in the solution are not described in detail here. It should be pointed out that for those skilled in the art, without departing from the technical solution of the present invention, several variations and improvements can be made, which should also be regarded as the scope of protection of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the description can be used to interpret the content of the claims.

Claims

1. A toothpaste containing macadamia polypeptide, characterized in that: The raw materials include, by weight: 2-3 parts of macadamia nut polypeptide, 15-30 parts of abrasive, 10-75 parts of moisturizer, 0.5-5 parts of surfactant, 0.1-5 parts of sweetener, 0.5-3 parts of thickener, and 0.1-2 parts of flavor; The friction agent includes a conventional friction agent and a natural friction agent; the conventional friction agent includes at least one of silicon dioxide, calcium bicarbonate and calcium hydrogen phosphate dihydrate; and the natural friction agent is macadamia nut shell powder.

2. The toothpaste containing macadamia polypeptide according to claim 1, characterized in that The macadamia nut polypeptide is prepared by the following method: removing the oil from the kernel of macadamia nuts through a cold pressing process to obtain fruit meal; soaking the fruit meal in anhydrous ethanol to defatted, and then drying to constant weight; crushing the dried fruit meal to obtain fruit meal powder; boiling the fruit meal powder in boiling water, and after cooling, enzymatically hydrolyzing the fruit meal powder with papain; after the enzymatic hydrolysis is completed, boiling the mixture and then centrifuging to collect the supernatant; and drying the supernatant to obtain a powdered macadamia nut polypeptide with a moisture content of less than 5%.

3. The toothpaste containing macadamia polypeptide according to claim 2, characterized in that The method for enzymatic hydrolysis of fruit meal powder with papain is as follows: the fruit meal powder is dispersed in boiling water and kept for 10 minutes, then the boiling water is cooled to 37°C, the pH value is adjusted to 7.0, and papain is added to obtain an enzymatic hydrolysis system; the mass percentage of fruit meal powder in the enzymatic hydrolysis system is 20%, the mass percentage of papain in the fruit meal powder is 0.5%, and the enzymatic hydrolysis is carried out at a constant temperature for 8 hours.

4. The toothpaste containing macadamia polypeptide according to claim 1, characterized in that The moisture content of the macadamia nut shell powder is ≤5%; and the particle size is 20-100 μm.

5. The toothpaste containing macadamia polypeptide according to claim 1, characterized in that The moisturizing agent comprises a first moisturizing agent and a second moisturizing agent; the first moisturizing agent is glycerin and / or sorbitol; and the second moisturizing agent is polyethylene glycol.

6. The toothpaste containing macadamia polypeptide according to claim 1, characterized in that The thickener includes at least one of sodium carboxymethyl cellulose, carrageenan, guar gum and xanthan gum.

7. The toothpaste containing macadamia polypeptide according to claim 1, characterized in that The surfactant includes at least one of sodium lauryl sulfate, cocoyl alanine triacetate ammonium, sodium laureth sulfate and cocoyl glutamate triethanolamine.

8. The toothpaste containing macadamia polypeptide according to claim 1, characterized in that The sweetener includes at least one of saccharin sodium, aspartame, stevia, sucralose, and xylitol.

9. A toothpaste containing macadamia polypeptide according to any one of claims 1 to 8, characterized in that: The raw materials include, by weight: 3 parts of macadamia nut polypeptide, 15 parts of abrasive, 55 parts of moisturizer, 3 parts of surfactant, 1 part of sweetener, 2.5 parts of thickener, and 0.5 parts of flavor; The mass ratio of conventional friction agent to natural friction agent is 1:

2.

10. The method for preparing a toothpaste containing macadamia polypeptide according to any one of claims 1 to 9, characterized in that: Mix macadamia nut shell powder and water in a ratio of 5:1 and mix well; after 20-40 minutes, add a second moisturizer and mix with the moistened macadamia nut shell powder to obtain a mixture A; Dispersing the thickener in the first moisturizer to obtain mixture B; dispersing the macadamia polypeptide in mixture B and allowing to stand for 20-40 minutes to obtain mixture C; Adding a surfactant, a sweetener, and the remaining water to mixture C to obtain mixture D; Mix mixture A and mixture D, add conventional friction agent, stir and homogenize in a paste making machine and evacuate; Add essence, stir and homogenize, and deaerate to obtain toothpaste.

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