Carthamus tinctorius polysaccharide nanoemulsion moisturizing spray and preparation method thereof

Abstract

The invention is suitable for the technical field of cosmetics, provides a safflower polysaccharide nanoemulsion moisturizing spray and a preparation method thereof, and relates to a safflower extract with a moisturizing effect, and the safflower extract is prepared by taking safflower polysaccharide (SPS) as a core active component through heating reflux extraction. Extracts of traditional Chinese medicinal materials such as saussurea involucrata, scutellaria baicalensis and liquorice are also added, various natural plant essence is adopted, and the components have a synergistic effect, so that the moisturizing, relieving and repairing effects of the composition are further enhanced. In the preparation process, a homogenate nanoemulsion preparation method is adopted, the unique nanoemulsion preparation method enables the product to efficiently package hydrophilic and hydrophobic active ingredients, and the solubility and stability of the ingredients are remarkably improved. Through scientific formula design and process optimization, the composition provided by the invention can effectively improve the problems of dry skin, rough skin and the like, and provides a natural, safe and efficient skin care choice for users.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to a safflower polysaccharide nanoemulsion moisturizing spray and a preparation method thereof. Background Art

[0002] Safflower polysaccharide (SPS), a key active ingredient in safflower, is a homogeneous heteropolysaccharide with diverse pharmacological activities, including anti-tumor, immunomodulatory, and anticoagulant properties. Its molecules contain numerous hydrophilic groups, such as hydroxyl and carboxyl groups, which can form hydrogen bonds with water molecules, exerting a moisturizing effect. Furthermore, safflower polysaccharide forms a gelatinous matrix with other components in the skin, exhibiting excellent film-forming properties that provide moisturizing and protection.

[0003] However, despite the significant moisturizing potential of safflower polysaccharide, its application in the cosmetics field currently faces challenges. Nanoemulsions, as submicron colloidal dispersion systems, have been widely used to improve the solubility, stability, and permeability of active ingredients. However, there is no research in the prior art on the use of safflower polysaccharide in nanoemulsion formulations for cosmetic applications. The shortcomings of the existing technology are that it fails to fully utilize the moisturizing properties of safflower polysaccharide and lacks the development of effective nanoemulsion formulations, which limits its application in moisturizing cosmetics.

[0004] Therefore, it is necessary to provide a safflower polysaccharide nanoemulsion moisturizing spray and a preparation method thereof to solve the above technical problems. Summary of the Invention

[0005] The invention provides a safflower polysaccharide nanoemulsion moisturizing spray and a preparation method thereof, aiming to solve the technical problem of insufficient application of safflower polysaccharide in cosmetics in the prior art.

[0006] The present invention relates to a safflower extract with moisturizing effect. The extract takes safflower polysaccharide (SPS) as a core active ingredient and is prepared by heating and refluxing extraction.

[0007] The present invention also adds extracts of Chinese medicinal materials such as Tianshan Snow Lotus, Scutellaria baicalensis, and Licorice, as well as a variety of natural plant essences. These ingredients work synergistically to further enhance the moisturizing, soothing and repairing effects of the composition.

[0008] The present invention provides a safflower polysaccharide nanoemulsion moisturizing spray, which comprises the following components by mass fraction:

[0009] Polyoxyethylene castor oil, Tween-80, PEG-400 and safflower seed oil 0.3%,

[0010] 1,4-Butanediol 3%,

[0011] Safflower polysaccharide 5% to 20%,

[0012] Snow lotus extract 3%,

[0013] Licorice extract, Scutellaria baicalensis extract 2%,

[0014] Niacinamide 0.2%,

[0015] Preservative 1%,

[0016] Triethanolamine 0.2%.

[0017] Preferably, the moisturizing ingredient, the antibacterial and whitening ingredient, and the whitening ingredient are: safflower polysaccharide, 1,4-butanediol, scutellaria baicalensis extract, licorice extract, snow lotus extract, and niacinamide.

[0018] The present invention also provides a method for preparing a safflower polysaccharide nanoemulsion moisturizing spray, comprising the following steps:

[0019] S1, preparing a basic nanoemulsion by homogenizing polyoxyethylene castor oil, Tween-80, PEG-400 and safflower seed oil;

[0020] S2, adding the moisturizing component, antibacterial and whitening component and the whitening component to the basic nanoemulsion and continuing to homogenize;

[0021] S3, add preservatives, and after the system cools down, add triethanolamine and flavor;

[0022] The average particle size of the basic nanoemulsion is 33.07 nm, the polydispersity index PDI test result is 0.196, and the average potential of the nanoemulsion is -16.7 mV.

[0023] Preferably, the preparation of the basic nanoemulsion comprises the following steps:

[0024] S1.1. Reagent Mixing: Heat polyoxyethylene castor oil in an oven at 50°C for 2-3 min to dissolve it into a liquid. Add Tween-80, PEG-400, and safflower oil in sequence, stirring thoroughly after each addition. Ultrasonicate for 10 min after adding the safflower oil. Subsequently, add 50 mL of distilled water while stirring, and mechanically stir at 1000 rpm for 10 min.

[0025] S1.2. Primary emulsification: homogenize the mixed solution obtained in step S1.1 at 2500 rpm for 50 min in an ice bath;

[0026] S1.3. Fine emulsification: The emulsion obtained in step S1.2 was disrupted and emulsified in an ice bath using a cell disruptor for 30 min to obtain a basic nanoemulsion.

[0027] The nanoemulsion preparation method using the homogenization method enables the product to efficiently encapsulate hydrophilic and hydrophobic active ingredients, significantly improving the solubility and stability of these ingredients; at the same time, it can significantly enhance the permeability of the active ingredients, thereby improving the product's absorption rate and moisturizing ability.

[0028] Preferably, the step of adding the moisturizing component, the antibacterial and whitening component and the whitening component comprises:

[0029] Dissolve safflower polysaccharide, 1,4-butanediol, scutellaria baicalensis extract, licorice extract, snow lotus extract and niacinamide in the aqueous phase, stir and dissolve, and then add to the basic nanoemulsion in proportion;

[0030] The cells were first emulsified with a high-speed homogenizer for 15 min and then disrupted with a cell disruptor for 15 min to prepare safflower polysaccharide nanoemulsion.

[0031] Then, the preservative is added while magnetic stirring, and when the system temperature is lower than 40°C, triethanolamine and flavor are added to adjust the pH to prevent the flavor from volatilizing.

[0032] After cooling and emulsification, the emulsified system is stirred and allowed to cool to near room temperature before filling.

[0033] Compared with related technologies, the safflower polysaccharide nanoemulsion moisturizing spray and its preparation method provided by the present invention have the following beneficial effects:

[0034] 1. The present invention adopts a composite emulsifier to emulsify the oil phase safflower seed oil, and no stratification phenomenon occurs after standing for a period of time, thereby improving the stability of the nanoemulsion and improving the greasy feeling and absorbency of the product.

[0035] 2. Through scientific formula design and process optimization, the composition of the present invention can effectively improve problems such as dry and rough skin, providing users with a natural, safe and efficient skin care option.

[0036] 3. The present invention also provides the stability and moisturizing results of safflower polysaccharide nanoemulsion moisturizing sprays with different safflower polysaccharide contents determined based on stability tests, skin moisture content determinations, and sensory evaluations. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 The graphs of moisture absorption rates of glycerol, sodium hyaluronate, and safflower polysaccharide over time at relative humidity of 43% (A) and 81% (B) are shown;

[0038] Figure 2 This is a graph showing the moisturizing rates of glycerin, sodium hyaluronate, and safflower polysaccharide after being placed under dry silica gel for 48 hours;

[0039] Figure 3 This is the appearance of the nanoemulsion and its Tyndall phenomenon diagram;

[0040] Figure 4 is the nanoemulsion particle size diagram;

[0041] Figure 5 is the potential distribution diagram of the nanoemulsion;

[0042] Figure 6 This is the morphological diagram of the stability experiment of 7 successful formulations;

[0043] Figure 7 This is a bar graph showing the results of measuring skin moisture content after using safflower polysaccharide nanoemulsion moisturizing spray;

[0044] Figure 8 It is a radar chart of sensory evaluation of samples. DETAILED DESCRIPTION

[0045] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0046] The present invention relates to a safflower extract with moisturizing effect. The extract takes safflower polysaccharide (SPS) as a core active ingredient and is prepared by heating and refluxing extraction.

[0047] The present invention also adds extracts of Chinese medicinal materials such as Tianshan Snow Lotus, Scutellaria baicalensis, and Licorice, as well as a variety of natural plant essences. These ingredients work synergistically to further enhance the moisturizing, soothing and repairing effects of the composition.

[0048] The present invention provides a safflower polysaccharide nanoemulsion moisturizing spray, which comprises the following ingredients, calculated by mass fraction: 0.3% of polyoxyethylene castor oil, Tween-80, PEG-400 and safflower seed oil, 3% of 1,4-butylene glycol, 5%-20% of safflower polysaccharide, 3% of snow lotus extract, 2% of liquorice extract and scutellaria baicalensis extract, 0.2% of niacinamide, 1% of preservative, and 0.2% of triethanolamine; wherein the moisturizing ingredient, the antibacterial and whitening ingredient, and the whitening ingredient are: safflower polysaccharide, 1,4-butylene glycol, scutellaria baicalensis extract, liquorice extract, snow lotus extract, and niacinamide.

[0049] The preparation method of the safflower polysaccharide nanoemulsion moisturizing spray comprises the following steps:

[0050] S1, preparing a basic nanoemulsion by homogenizing polyoxyethylene castor oil, Tween-80, PEG-400 and safflower seed oil;

[0051] S2, adding the moisturizing component, antibacterial and whitening component and the whitening component to the basic nanoemulsion and continuing to homogenize;

[0052] S3, add preservatives, and after the system cools down, add triethanolamine and flavor;

[0053] The average particle size of the basic nanoemulsion is 33.07 nm, the polydispersity index PDI test result is 0.196, and the average potential of the nanoemulsion is -16.7 mV.

[0054] Among them, the nanoemulsion preparation method using the homogenization method can enable the product to efficiently encapsulate hydrophilic and hydrophobic active ingredients, significantly improving the solubility and stability of these ingredients; at the same time, it can significantly enhance the permeability of the active ingredients, thereby improving the absorption rate and moisturizing ability of the product. The preparation of the basic nanoemulsion includes the following steps:

[0055] S1.1. Reagent Mixing: Heat polyoxyethylene castor oil in an oven at 50°C for 2-3 min to dissolve it into a liquid. Add Tween-80, PEG-400, and safflower oil in sequence, stirring thoroughly after each addition. Ultrasonicate for 10 min after adding the safflower oil. Subsequently, add 50 mL of distilled water while stirring, and mechanically stir at 1000 rpm for 10 min.

[0056] S1.2. Primary emulsification: homogenize the mixed solution obtained in step S1.1 at 2500 rpm for 50 min in an ice bath;

[0057] S1.3. Fine emulsification: The emulsion obtained in step S1.2 was disrupted and emulsified in an ice bath using a cell disruptor for 30 min to obtain a basic nanoemulsion.

[0058] The steps of adding the moisturizing component, the antibacterial and whitening component and the whitening component include:

[0059] Dissolve safflower polysaccharide, 1,4-butanediol, scutellaria baicalensis extract, licorice extract, snow lotus extract and niacinamide in the aqueous phase, stir and dissolve, and then add to the basic nanoemulsion in proportion;

[0060] The cells were first emulsified with a high-speed homogenizer for 15 min and then disrupted with a cell disruptor for 15 min to prepare safflower polysaccharide nanoemulsion.

[0061] Then, add the preservative while magnetically stirring. When the system temperature is below 40°C, add triethanolamine and flavor to adjust the pH to prevent the flavor from volatilizing. After cooling and emulsification, stir the emulsified system and allow it to cool to near room temperature before filling.

[0062] In the above preparation method, the present invention uses a composite emulsifier to emulsify the oil phase safflower seed oil, and no stratification occurs after standing for a period of time, thereby improving the stability of the nanoemulsion and improving the greasy feel and absorbency of the product.

[0063] In the preparation method provided by the present invention, through scientific formula design and process optimization, the composition of the present invention can effectively improve problems such as dry and rough skin, providing users with a natural, safe and efficient skin care option.

[0064] The present invention will provide the stability and moisturizing results of safflower polysaccharide nanoemulsion moisturizing sprays with different safflower polysaccharide contents determined by stability tests, skin moisture content determination, and sensory evaluation in specific embodiments.

[0065] Example 1

[0066] A method for preparing a safflower polysaccharide nanoemulsion moisturizing spray comprises the following steps:

[0067] The first step is to mix the reagents. Place the weighed polyoxyethylene castor oil in a 50°C oven for 2-3 minutes to melt until it becomes liquid. Then, add Tween-80, PEG-400, and safflower oil in sequence, ensuring each reagent is thoroughly stirred. After the seed oil is added, ultrasonicate for 10 minutes to ensure uniform mixing. Finally, add 50 mL of distilled water while stirring, and mechanically stir at 1000 rpm for 10 minutes.

[0068] The second step is primary (coarse) emulsification. The mixed solution obtained in the previous step is homogenized in an ice bath in a high-speed homogenizer at 2500 rpm for 50 min.

[0069] The third step is fine emulsification. The homogenized emulsion is crushed and fine emulsified on a cell crusher in an ice bath for 30 minutes to obtain a basic nanoemulsion.

[0070] In the fourth step, safflower polysaccharide, 1,4-butanediol, scutellaria baicalensis extract, licorice extract, snow lotus extract, and niacinamide were dissolved in the aqueous phase and stirred to dissolve, and then added to the nanoemulsion base component phase according to the proportion, emulsified by a high-speed homogenizer for 15 minutes, and then disrupted on a cell disruptor for 15 minutes to obtain safflower polysaccharide nanoemulsion.

[0071] The fifth step is to add the preservative while stirring using a magnetic stirrer to obtain the maximum concentration of the preservative in the water and to distribute it evenly.

[0072] Step 6: When the system temperature is below 40°C, add triethanolamine and flavor to adjust the pH to prevent the flavor from volatilizing. After cooling and emulsification, stir the emulsified system and allow it to cool to near room temperature before filling.

[0073] Performance Testing

[0074] (1) Hygroscopicity and moisturizing properties of safflower polysaccharide

[0075] Sodium hyaluronate and safflower polysaccharide were ground into 80 mesh and placed in an oven along with glycerin at 40°C until constant weight was reached. The dried sample was used directly for the hygroscopicity test. For the moisture retention test, deionized water (10% of the sample mass) was added to the dried sample.

[0076] Accurately weigh 0.5g of processed safflower polysaccharide, glycerol, and sodium hyaluronate in two portions, and place them in weighing bottles with a diameter of 3cm. Place the weighing bottles in two desiccators, one of which contains a saturated ammonium sulfate solution (relative humidity RH = 81%), and the other contains a saturated sodium carbonate solution (relative humidity RH = 43%). The samples were placed in the desiccators for 3, 6, 12, 24, 36, 48, 60, and 72 hours, respectively. Weigh the mass of the sample before placement (M0) and the mass after placement (M n Each experiment was repeated three times, and the moisture absorption rate was calculated according to the following formula: Moisture absorption rate / % = 100 (M n -M0) / M0.

[0077] At room temperature, accurately weigh three 0.5g samples, place them in a weighing bottle with a diameter of 3cm, add 10% of the sample amount of deionized water, and place them in a desiccator filled with blue silica gel. Then place them in a comprehensive drug stability test box (25℃) for moisture retention test, take them out after 48 hours, and weigh them. Weigh the mass W after placement n And the mass W0 before placement. Calculate the moisturizing rate according to the following formula: Moisturizing rate / % = 100 (W n -W0) / W0.

[0078] like Figure 1 The hygroscopic curves shown in Figure 1 depict the hygroscopic properties of safflower polysaccharide, sodium hyaluronate, and glycerol at 43% and 81% relative humidity. All three substances initially exhibited rapid water absorption rates, but after 24 hours, the water absorption rates of safflower polysaccharide and sodium hyaluronate decreased significantly. At 43% relative humidity, the hygroscopic curves of the three substances nearly overlapped from 0 to 6 hours, indicating similar hygroscopic behavior during this period. However, over time, from 6 to 24 hours, the curves began to diverge, revealing significant differences in hygroscopic rates. By 24 hours, the hygroscopic rates of safflower polysaccharide and sodium hyaluronate plateaued, suggesting that they may have reached hygroscopic equilibrium. At 81% relative humidity, the hygroscopic curves of the three substances exhibited similar trends as those at 43%. Safflower polysaccharide exhibited a moderate hygroscopic capacity under both humidity conditions. Because both safflower polysaccharide and sodium hyaluronate contain macromolecular structures with multiple hydroxy groups, they are able to absorb water through hydrogen bonding. However, once the surface of these substances absorbs water, they may swell, thereby hindering the further absorption of water by the internal molecules. Therefore, compared with glycerol, safflower polysaccharide and sodium hyaluronate are more likely to reach moisture absorption equilibrium earlier.

[0079] like Figure 2 As shown, this figure depicts the moisturizing properties of safflower polysaccharide after being placed in a dry silica gel environment for 48 hours. Figure 2 It can be seen that under these dry conditions, glycerin and hyaluronic acid exhibited a high moisturizing effect. In contrast, safflower polysaccharide exhibited significantly lower water loss than purified water. After 48 hours, even though the purified water had completely evaporated, safflower polysaccharide still retained a 10.246% moisture retention rate, demonstrating its considerable moisturizing capacity. This result suggests that safflower polysaccharide, as a humectant, can provide a certain degree of moisture retention in dry environments.

[0080] (2) Nanoemulsion prescription screening

[0081] In nanoemulsion formulations, the selection of oil and lipid is the most critical parameter, while the selection of other components, such as surfactants and cosurfactants, is equally important. This study used instruments such as a high-speed homogenizer and ultrasonic cell disruptor to sequentially add an emulsifier, a co-emulsifier, and blank safflower seed oil as the oil phase, according to the dosages specified in Table 3. After thorough stirring, 50 mL of purified water was added and the mixture was emulsified in a high-speed homogenizer for 30 minutes to prepare a blank safflower seed oil nanoemulsion. This was then illuminated with an infrared laser pen, and the degree of light scattering was used as an indicator to screen the optimal formulation. This selected formulation was then modified by varying the reagent mixing process, mixing method, and homogenization and sonication time, with particle size and polydispersity index (PDI) as references to develop a nanoemulsion with smaller and more stable particle sizes. This was then applied to the development of a safflower polysaccharide nanoemulsion moisturizing spray.

[0082] Table 1 Nanoemulsion formulation screening composition

[0083]

[0084]

[0085] 1 mL of nanoemulsion sample was pipetted onto the wall of a Malvern quartz cuvette. The cuvette was then placed in a laser particle size analyzer with zeta potential and absolute molecular weight. Particle size and polydispersity index (PDI) were selected and analyzed to identify nanoemulsion preparation methods that met the desired objectives. All samples were measured three times at 25°C.

[0086] After aspirating 1 mL of nanoemulsion and diluting it 15-fold, 1 mL was placed in a Malvern potential cell. The Malvern potential cell was then placed in a laser particle size zeta potential and absolute molecular weight analyzer. The potential detection program was selected and the potential detection was performed. All samples were measured three times at 25°C.

[0087] Based on the basic nanoemulsion formula ingredients, following conventional R&D processes, we carefully selected appropriate raw materials from multiple perspectives, including emulsification, thickening, antioxidants, preservatives, sensory enhancement, and system safety, and precisely determined the dosage ratios of each raw material. Using a single-factor control method, we manipulated the content of safflower polysaccharide in the nanoemulsion moisturizing spray. We also designed the following seven formulations for comparison with glycerin and sodium hyaluronate groups.

[0088] Table 2 Formula of safflower polysaccharide nanoemulsion moisturizing spray

[0089]

[0090]

[0091] The basic nanoemulsion formulation was screened to determine the amounts of oil phase, emulsifier, and co-emulsifier in the formulation. The screening results are shown in Table 2. As can be seen from Table 2, the particle size and PDI of the nanoemulsion prepared by formulation 5 were better than those of the other formulations. Therefore, the optimal basic nanoemulsion formulation was determined to be 0.3 g of oil phase (safflower seed oil), 0.3 g + 0.3 g of emulsifier (castor oil + Tween-80), and 0.3 g of co-emulsifier (PEG400).

[0092] Table 3 Nanoemulsion prescription screening results

[0093]

[0094] As can be seen from the data in the table, the particle size and PDI of Formulation 5 are still far from the expected target. Subsequently, using particle size and PDI as evaluation indicators, the final preparation scheme was explored by varying the reagent mixing process, mixing method, and homogenization and sonication time. The specific preparation steps are as follows:

[0095] The first step is reagent mixing. Place the weighed polyoxyethylene castor oil in a 50°C oven for 2-3 minutes to melt it into a liquid, then add Tween-80, PEG-400 and safflower seed oil in sequence, ensuring that each added reagent has been fully stirred, and ultrasonicate for 10 minutes after the seed oil is added and stirred to mix it evenly. Finally, add 50mL of distilled water while stirring, and mechanically stir at 1000r / min for 10 minutes. The second step is primary (coarse) emulsification. Homogenize the mixed solution obtained in the previous step in an ice bath in a high-speed homogenizer at 2500r / min for 50 minutes. The third step is fine emulsification. The homogenized emulsion is crushed and finely emulsified on a cell crusher in an ice bath for 30 minutes to obtain a basic nanoemulsion. The finished product is shown in FIG. Figure 3 .

[0096] Depend on Figure 3It can be seen that the basic nanoemulsion is a light milky white, slightly yellowish clear emulsion. After irradiation with an infrared laser pen, there is an obvious Tyndall phenomenon, which preliminarily indicates that the nanoemulsion particle size is small and evenly dispersed.

[0097] Nanoemulsion particle size and polydispersity index test results:

[0098] Depend on Figure 4 The average size of the basic nanoemulsion is about 33.07 nm, and the PDI is 0.196, which further proves that the basic nanoemulsion has a small particle size and is evenly dispersed.

[0099] Nanoemulsion potential test results:

[0100] The potential of the basic nanoemulsion was tested by the laser particle size Zeta potential and absolute molecular weight analyzer. The test results are shown in Figure 5 .

[0101] Depend on Figure 5 It can be seen that the average potential of the basic nanoemulsion is -16.7 mV, and the potential is relatively concentrated, which proves that the nanoparticles in the nanoemulsion can be dispersed from each other without aggregation, and the nanoemulsion has good stability.

[0102] (3) Product stability test

[0103] Heat and cold resistance experiments:

[0104] Equal amounts of the nanoemulsion were placed in three 15ml centrifuge tubes. The tubes were then placed at -10°C, -5°C, 25°C, and 40°C for 24 hours. After returning to room temperature, the nanoemulsions were observed for abnormalities such as turbidity or stratification. The experiment was repeated three times for each temperature condition.

[0105] Centrifugal stability test:

[0106] Equal amounts of the nanoemulsion were placed in three 15 ml centrifuge tubes and centrifuged at 1000 rpm, 2000 rpm, and 3000 rpm, respectively. After centrifugation, the nanoemulsions were observed for turbidity, stratification, or demulsification.

[0107] Dilution stability test:

[0108] Take equal amounts of nanoemulsion and place them in three 15 ml centrifuge tubes, dilute them to 5 times, 10 times, and 15 times the volume, respectively, and observe whether the diluted nanoemulsion becomes turbid, stratified, or demulsified.

[0109] like Figure 6As shown, after the 7 samples were successfully prepared according to the method of 3.4.2, they all showed a uniform translucent liquid appearance. In the heat and cold resistance experiment, the 7 samples did not show stratification, demulsification and other phenomena under different temperature conditions. After the dilution test with different dilution multiples, the 7 samples did not show stratification, demulsification and other phenomena. During the centrifugation experiment, at a speed of 2000r / min of the centrifuge, Formula 5 and Formula 6 showed slight stratification and turbidity, and at a speed of 3000r / min, Formula 5 and Formula 6 showed stratification, indicating that after the safflower polysaccharide content exceeded 10%, the product became unstable. After testing, the pH values of the 7 samples were all within the theoretical range.

[0110] (4) Skin moisture test and sensory evaluation results of the product

[0111] The successfully formulated product was selected from the above experiments, and 14 volunteers aged 20 to 35 were recruited as subjects through in vivo testing. Before the experiment began, the subjects were required to clean and dry their left and right forearms, and then let them stand for 30 minutes in an environment with a temperature of 22°C and a relative humidity of 50% to 65% to reach equilibrium. A 3cm×3cm test area was marked on the left and right forearms of the subjects. After spraying two pumps of moisturizing spray on the test area, the skin moisture content was measured using a skin moisture content tester at 0.5 hours, 1 hour, 2 hours, 3 hours, 6 hours, 9 hours and 12 hours. Three different parts of each test area were selected for measurement, and the average value was taken as the result.

[0112] Skin moisture content test:

[0113] like Figure 7 The changes in skin moisture content of the subjects shown are as follows 0.5 hours, 1 hour, 2 hours, 3 hours, 6 hours, 9 hours and 12 hours after using the safflower polysaccharide nanoemulsion moisturizing spray. The analysis results showed that over time, the skin moisture content of all test sample areas increased compared with before use. The obtained data were analyzed using GraphPad Prism 10.4.2 software, and the probability P value after using different concentrations of safflower polysaccharide spray for 0.5 hours, 1 hour, 2 hours, 3 hours, 6 hours, 9 hours, and 12 hours was calculated using one-way analysis of variance; when P≤0.05, it indicates that there is a significant difference in the effect of different concentrations of safflower polysaccharide nanoemulsion on skin moisture content. The results showed that after using 5%, 10%, 15%, and 20% safflower polysaccharide nanoemulsion spray for 0.5 hours, 1 hour, 2 hours, 3 hours, 6 hours, 9 hours, and 12 hours, the test area had a significant difference in P value after using 5%, 10%, 15%, and 20% safflower polysaccharide nanoemulsion spray for 0.5 hours, 1 hour, 2 hours, 3 hours, 6 hours, 9 hours, and 12 hours. 0.5 =0.890, P1=0.7808, P2=0.1632, P3=0.0791, P6=0.2326, P9=0.0541, P 12=0.3206, all greater than 0.05, indicating that within 12 hours, there was no statistically significant difference in the effects of different concentrations of safflower polysaccharide nanoemulsion spray on skin moisture content.

[0114] Further analysis showed that after 0.5 hours, 1 hour, 2 hours, 3 hours, 6 hours, 9 hours, and 12 hours of spraying with blank spray and 5% safflower polysaccharide nanoemulsion, the significance P 0.5 =0.0193, P1=0.0344, P2=0.0259, P3=0.0099, P6=0.0006, P9=0.4295, P 12 =0.0092, both less than 0.05, confirming that the addition of safflower polysaccharide to the spray significantly enhances its moisturizing effect. Furthermore, the figure shows that within the first two hours of using the safflower polysaccharide nanoemulsion spray, the skin moisture content in the test area was significantly higher than that in the areas sprayed with sodium hyaluronate and glycerin. This further confirms the excellent moisturizing properties of the safflower polysaccharide nanoemulsion moisturizing spray. Therefore, the skin moisture content test results indicate that the safflower polysaccharide nanoemulsion moisturizing spray can significantly increase skin moisture content in the short term.

[0115] Sensory evaluation plays a crucial role in the early and mid-stages of cosmetic formulation development, helping to select high-quality product formulations from a wide range of candidates. This evaluation process allows for detailed analysis and comparison of the sensory properties of formulations, ensuring that the final product meets consumer needs and expectations. This study employed a single-factor control approach, using sensitivity, fragrance, smoothness, moisturizing properties, greasiness, stickiness, and absorbability of the moisturizing spray as evaluation criteria (see Table 4 for specific evaluation criteria). The experimental group was compared with a blank group, a glycerol group, and a sodium hyaluronate group to investigate the effect of safflower polysaccharide content on the sensory experience of the safflower polysaccharide nanoemulsion moisturizing spray. Data were analyzed using a one-way analysis of variance (ANOVA) using GraphPadPrism 10.4.2 software.

[0116] Table 4 Sensory evaluation standards for moisturizing spray

[0117]

[0118]

[0119] The sensitivity, fragrance, fineness, moisturizing, greasiness, stickiness and absorbability of the moisturizing spray were used as evaluation indicators. The experimental group was compared with the blank group, glycerin group and sodium hyaluronate group. Based on the sensory evaluation scores of 14 subjects, a radar chart of the sample sensory evaluation was drawn using Origin Pro 2025 drawing software to show the performance of different formulas in multiple attributes. Figure 8This figure shows that Formula 1 (glycerin group), Formula 2 (blank group), and Formula 7 (sodium hyaluronate group) performed well in sensitivity, fragrance, and stickiness, but performed averagely in moisturizing. This result is generally consistent with the results of skin moisture content measurement in 4.5.1. Formulas 5 and 6 performed averagely in terms of absorbability and stickiness, indicating that the addition of 15% and 20% safflower polysaccharide to the spray can make the product sticky. This is due to the viscosity and adhesiveness of safflower polysaccharide. Excessive addition can reduce the product experience.

[0120] In general, among the six indicators, the highest comprehensive score of sensory evaluation was obtained in formula 3 (5% safflower polysaccharide content). Comprehensive analysis of the results showed that the nanoemulsion moisturizing spray containing 5% safflower polysaccharide was the optimal formula.

[0121] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the scope of protection of the invention. Obviously, the embodiments described are only some embodiments of the present invention, rather than all embodiments. Based on these embodiments, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in this field can still combine, add, delete or make other adjustments to the features in the various embodiments of the present invention according to the circumstances without conflict, without making creative work, so as to obtain different other technical solutions that do not deviate from the concept of the present invention in essence, and these technical solutions also fall within the scope of protection of the present invention.

Claims

1. A safflower polysaccharide nanoemulsion moisturizing spray, characterized in that: According to mass fraction, it includes the following ingredients: Polyoxyethylene castor oil, Tween-80, PEG-400 and safflower seed oil 0.3%, 1,4-Butanediol 3%, Safflower polysaccharide 5% to 20%, Snow lotus extract 3%, Licorice extract, Scutellaria baicalensis extract 2%, Niacinamide 0.2%, Preservative 1%, Triethanolamine 0.2%.

2. The safflower polysaccharide nanoemulsion moisturizing spray and preparation method thereof according to claim 1, characterized in that: The moisturizing ingredients, antibacterial and whitening ingredients are: safflower polysaccharide, 1,4-butanediol, scutellaria baicalensis extract, licorice extract, snow lotus extract, and niacinamide.

3. A method for preparing a safflower polysaccharide nanoemulsion moisturizing spray, characterized in that: The method comprises the safflower polysaccharide nanoemulsion moisturizing spray according to any one of claims 1 to 2, and the preparation method comprises the following steps: S1, preparing a basic nanoemulsion by homogenizing polyoxyethylene castor oil, Tween-80, PEG-400 and safflower seed oil; S2, adding the moisturizing component, antibacterial and whitening component and the whitening component to the basic nanoemulsion and continuing to homogenize; S3, add preservatives, and after the system cools down, add triethanolamine and flavor; The average particle size of the basic nanoemulsion is 33.07 nm, the polydispersity index PDI test result is 0.196, and the average potential of the nanoemulsion is -16.7 mV.

4. The method for preparing the safflower polysaccharide nanoemulsion moisturizing spray according to claim 3, wherein: The preparation of the basic nanoemulsion comprises the following steps: S1.

1. Reagent Mixing: Heat polyoxyethylene castor oil in an oven at 50°C for 2-3 min to dissolve it into a liquid. Add Tween-80, PEG-400, and safflower oil in sequence, stirring thoroughly after each addition. Ultrasonicate for 10 min after adding the safflower oil. Subsequently, add 50 mL of distilled water while stirring, and mechanically stir at 1000 rpm for 10 min. S1.

2. Primary emulsification: homogenize the mixed solution obtained in step S1.1 at 2500 rpm for 50 min in an ice bath; S1.

3. Fine emulsification: The emulsion obtained in step S1.2 was disrupted and emulsified in an ice bath using a cell disruptor for 30 min to obtain a basic nanoemulsion.

5. The method for preparing the safflower polysaccharide nanoemulsion moisturizing spray according to claim 3, wherein: The steps of adding the moisturizing component, the antibacterial whitening component and the whitening component include: Dissolve safflower polysaccharide, 1,4-butanediol, scutellaria baicalensis extract, licorice extract, snow lotus extract and niacinamide in the aqueous phase, stir and dissolve, and then add to the basic nanoemulsion in proportion; The safflower polysaccharide nanoemulsion was prepared by emulsifying with a high-speed homogenizer for 15 minutes and then disrupting with a cell disruptor for 15 minutes. Then, the preservative is added while magnetic stirring, and when the system temperature is lower than 40°C, triethanolamine and flavor are added to adjust the pH to prevent the flavor from volatilizing.

6. The method for preparing the safflower polysaccharide nanoemulsion moisturizing spray according to claim 3, characterized in that: The oil phase safflower seed oil was emulsified with a composite emulsifier and no stratification occurred after standing.

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