Natural plant essential oil compound with antibacterial effect and preparation method thereof

Abstract

The invention relates to the technical field of essential oil, in particular to a natural plant essential oil compound with an antibacterial effect and a preparation method thereof.The natural plant essential oil compound with the antibacterial effect is prepared from, by mass, 70-80 parts of a base oil phase, 10-12 parts of an antibacterial active component and 12-14 parts of a function synergistic component, a synergistic antibacterial system is formed by innovatively fusing natural antibacterial components and functional synergistic components and combining staged mixing, ultrasonic dispersion and low-temperature ripening processes, and compared with the prior art, the natural mild characteristic is maintained, meanwhile, the antibacterial efficiency and the moisturizing and repairing effect are remarkably improved, and the skin care effect is good. The stability of the product is enhanced through technical means such as vacuum degassing, the preparation process is adaptive to a conventional production line, raw materials are environmentally friendly and degradable, and the product is suitable for the fields of skin care products, disinfection spray, medical auxiliary materials and the like and shows a wide prospect in promotion of green and healthy product innovation and multi-scene application.

Description

Technical Field

[0001] The present invention relates to the technical field of essential oils, and in particular to a natural plant essential oil compound with antibacterial efficacy and a preparation method thereof. Background Art

[0002] Natural plant essential oil compounds are composite preparations formed by scientifically proportioning two or more plant essential oils. Their core ingredients are natural volatile compounds with antibacterial activity, such as terpenes, phenols, and aldehydes. Common compositions include tea tree oil, eucalyptus oil, cinnamon oil, thyme oil, lavender oil, etc. These essential oils can enhance the antibacterial effect through synergistic effects. They are mainly used in antibacterial hand sanitizers, daily chemical products for skin care, wound disinfection dressings, food preservation, and environmental cleaning. They are green, safe, and degradable.

[0003] In the existing technology, natural plant essential oil compounds mostly use a single plant essential oil as the antibacterial ingredient, but its antibacterial effect is only targeted at specific bacterial species and has limited inhibitory effect on drug-resistant bacteria. When used in daily chemical and medical products, it relies on chemical preservatives such as triclosan and benzalkonium chloride. Although these preservatives are broad-spectrum and highly effective, they have problems such as cytotoxicity, sensitization and environmental pollution.

[0004] Therefore, according to the relevant technologies mentioned above, there is an urgent need to develop a natural plant essential oil compound with antibacterial efficacy and a preparation method thereof. Summary of the Invention

[0005] In view of this, the purpose of the present invention is to provide a natural plant essential oil compound with antibacterial efficacy and a preparation method thereof, so as to solve the problems of narrow antibacterial spectrum, cytotoxicity, sensitization and environmental pollution in the prior art.

[0006] Based on the above objectives, the present invention provides a natural plant essential oil compound with antibacterial efficacy and a preparation method thereof.

[0007] A natural plant essential oil compound with antibacterial efficacy is composed of the following components in parts by mass: 70-80 parts of a base oil phase, 10-12 parts of an antibacterial active component, and 12-14 parts of a function-enhancing component.

[0008] Preferably, the base oil phase is prepared from sweet almond oil, jojoba oil, coconut oil and calendula infused oil.

[0009] Preferably, the base oil phase is prepared as follows:

[0010] Place sweet almond oil, jojoba oil and coconut oil in a stainless steel reactor, heat to 35-40°C, stir for 8-12 minutes at 150-250 rpm, add calendula infused oil, stir for 3-5 minutes, filter with a 5 μm filter membrane to remove impurities, and cool to 20-30°C to obtain a base oil phase;

[0011] By stirring at a constant temperature of 35-40℃, it not only avoids oil oxidation or essential oil volatilization caused by high temperature, but also promotes the full integration of bases such as sweet almond oil and coconut oil to ensure the stability of the moisturizing factor.

[0012] Preferably, the mass ratio of the sweet almond oil, jojoba oil, coconut oil and calendula infused oil is 9-10:2-3:1-2:1.

[0013] Preferably, the antibacterial active component is prepared from tea tree essential oil, lavender essential oil, thyme essential oil, oregano essential oil and eucalyptus essential oil.

[0014] Preferably, the steps for preparing the antibacterial active component are as follows:

[0015] Add oregano essential oil to a beaker, raise the temperature to 20-30°C, add thyme essential oil, tea tree essential oil, eucalyptus essential oil, and lavender essential oil, stir for 8-10 minutes at 400-600 rpm, protect from light, and let stand for 10-12 hours to obtain an antibacterial active component;

[0016] The synergistic effect of multiple components such as tea tree oil and lavender essential oil significantly enhances the antibacterial effect. At the same time, the use of mild bases such as sweet almond oil and calendula infused oil avoids the irritation of chemical additives on the skin, making it suitable for sensitive skin care.

[0017] In addition, the speed setting of 400-600rpm can reduce the loss of volatile components of essential oils while ensuring that active molecules such as oregano and tea tree are fully dispersed.

[0018] Preferably, the mass ratio of the oregano essential oil, thyme essential oil, tea tree essential oil, eucalyptus essential oil and lavender essential oil is 1:3-4:9-10:1-2:5-6.

[0019] Preferably, the function-enhancing component is prepared from borneol oil, frankincense oil, frankincense oil, bergamot essential oil, palmarosa oil and vitamin E oil;

[0020] By using functional synergistic components such as borneol oil and frankincense oil, combined with ultrasonic dispersion and homogenous emulsification technology, not only the permeability of the essential oil is enhanced, but also the product is given additional effects such as moisturizing and anti-inflammatory.

[0021] Preferably, the steps for preparing the functional enhancement component are as follows:

[0022] Step A1: Add frankincense oil to borneol oil, raise the temperature to 25-30° C., and ultrasonicate for 3-5 minutes at a frequency of 30-40 Hz to obtain a mixed solution;

[0023] Step A2: Add geranium essential oil, bergamot essential oil and palmarosa oil to the mixed solution, heat to 20-30°C, stir for 8-10 minutes, rotate at 700-800 rpm, then add vitamin E oil, stir for 3-5 minutes, and complete the reaction to obtain a functional enhancement component.

[0024] Preferably, the mass ratio of frankincense oil to borneol oil in step A1 is 1:0.9-1.1.

[0025] Preferably, the mass ratio of the geranium essential oil, bergamot essential oil, palmarosa oil, mixed solution and vitamin E oil in step A2 is 0.8-1.2:1:0.8-1.2:2-3:0.6-0.8.

[0026] Preferably, a method for preparing a natural plant essential oil compound with antibacterial efficacy is as follows:

[0027] Step S1: adding the antibacterial active component to the base oil phase, heating to 30-35°C, stirring for 10-15 minutes at a speed of 900-1000 rpm, adding the functional enhancement component, heating to 35-40°C, placing in a homogenizer, and emulsifying for 10-15 minutes to obtain an emulsion;

[0028] Step S2: adding the emulsion into a vacuum degassing tank, degassing for 18-22 minutes at a pressure of -0.08 MPa, filling with nitrogen, cooling to 0-5°C, and aging for 22-26 hours to obtain a natural plant essential oil compound with antibacterial efficacy;

[0029] Through process control such as staged mixing, vacuum degassing and low-temperature aging, the activity of ingredients is ensured to be stable and the emulsion is uniform. The setting of -0.08MPa vacuum degassing to remove emulsion bubbles can prevent oxidation and deterioration, and extend the shelf life to 18 months. In addition, through the setting of low-temperature aging at 0-5℃ for 22-26h, the molecules slowly self-assemble to form a stable network structure, ensuring that the product will not delaminate or precipitate under extreme temperatures.

[0030] Beneficial effects of the present invention:

[0031] The present invention provides a natural plant essential oil compound with antibacterial efficacy. The present invention forms a synergistic antibacterial system by innovatively integrating natural antibacterial ingredients such as tea tree essential oil and lavender essential oil with functional synergistic components such as borneol oil and frankincense oil, and combining phased mixing, ultrasonic dispersion and low-temperature maturation processes. At the same time, through scientific proportioning and process optimization, the activity of the ingredients is ensured to be stable and the permeability is enhanced. Compared with the existing technology, the present invention significantly improves the antibacterial efficiency and moisturizing and repairing effects while maintaining the natural and mild characteristics, and enhances the product stability through technical means such as vacuum degassing. Its preparation process is compatible with conventional production lines, and the raw materials are environmentally friendly and degradable. It is suitable for skin care products, disinfectant sprays, medical accessories and other fields, and shows broad prospects in promoting the innovation of green and healthy products and multi-scenario applications. DETAILED DESCRIPTION

[0032] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to specific embodiments.

[0033] Example 1: The steps for preparing the base oil phase are as follows:

[0034] 900 g of sweet almond oil, 200 g of jojoba oil and 100 g of coconut oil were placed in a stainless steel reactor, heated to 35°C, stirred for 12 min at 150 rpm, 100 g of calendula infused oil was added, stirred for 5 min, filtered with a 5 μm filter membrane to remove impurities, and cooled to 20°C to obtain a base oil phase.

[0035] Example 2: The steps for preparing the base oil phase are as follows:

[0036] 950 g of sweet almond oil, 250 g of jojoba oil and 150 g of coconut oil were placed in a stainless steel reactor, heated to 37°C, stirred for 10 min at 200 rpm, 100 g of calendula infused oil was added, stirred for 4 min, filtered with a 5 μm filter membrane to remove impurities, and cooled to 25°C to obtain a base oil phase.

[0037] Example 3: The steps for preparing the base oil phase are as follows:

[0038] 1000 g of sweet almond oil, 300 g of jojoba oil and 200 g of coconut oil were placed in a stainless steel reactor, heated to 40°C, stirred for 8 min at 250 rpm, 100 g of calendula infused oil was added, stirred for 3 min, filtered with a 5 μm filter membrane to remove impurities, and cooled to 30°C to obtain a base oil phase.

[0039] Example 4: The steps for preparing the antibacterial active component are as follows:

[0040] Add 100 g of oregano essential oil to a beaker, raise the temperature to 20°C, add 300 g of thyme essential oil, 900 g of tea tree essential oil, 100 g of eucalyptus essential oil and 500 g of lavender essential oil, stir for 8 minutes at 600 rpm, protect from light, and let stand for 10 hours to obtain antibacterial active components.

[0041] Example 5: The steps for preparing the antibacterial active component are as follows:

[0042] Add 100 g of oregano essential oil to a beaker, raise the temperature to 25°C, add 350 g of thyme essential oil, 950 g of tea tree essential oil, 150 g of eucalyptus essential oil and 550 g of lavender essential oil, stir for 9 minutes at 500 rpm, protect from light, and let stand for 11 hours to obtain antibacterial active components.

[0043] Example 6: The steps for preparing the antibacterial active component are as follows:

[0044] Add 100 g of oregano essential oil to a beaker, raise the temperature to 30°C, add 400 g of thyme essential oil, 1000 g of tea tree essential oil, 200 g of eucalyptus essential oil and 600 g of lavender essential oil, stir for 8 minutes at 600 rpm, protect from light, and let stand for 10 hours to obtain antibacterial active components.

[0045] Example 7: The steps for preparing the functional enhancement component are as follows:

[0046] S1: Add 100 g of frankincense oil to 90 g of borneol oil, heat to 25°C, and ultrasonicate for 5 min at a frequency of 30 Hz to obtain a mixed solution;

[0047] S2: Add 40g of geranium essential oil, 50g of bergamot essential oil and 40g of palmarosa oil to 100ml of the mixed solution, heat to 20°C, stir for 10min at 700rpm, then add 30g of vitamin E oil and stir for 3min. The reaction is complete to obtain a functional synergistic component.

[0048] Example 8: The steps for preparing the functional enhancement component are as follows:

[0049] S1: Add 100 g of frankincense oil to 100 g of borneol oil, heat to 27°C, and ultrasonicate for 4 min at a frequency of 35 Hz to obtain a mixed solution;

[0050] S2: Add 40 g of geranium essential oil, 40 g of bergamot essential oil and 40 g of palmarosa oil to 100 g of the mixed solution, raise the temperature to 25 ° C, stir for 9 minutes, rotate at 750 rpm, then add 30 g of vitamin E oil, stir for 4 minutes, and the reaction is completed to obtain a functional synergistic component.

[0051] Example 9: The steps for preparing the functional enhancement component are as follows:

[0052] S1: Add 100 g of frankincense oil to 110 g of borneol oil, heat to 30°C, and ultrasonicate for 3 min at a frequency of 40 Hz to obtain a mixed solution;

[0053] S2: Add 40g of geranium essential oil, 33g of bergamot essential oil and 40g of palmarosa oil to 100g of the mixed solution, raise the temperature to 30°C, stir for 8min, rotate at 800rpm, then add 27g of vitamin E oil, stir for 3min, and the reaction is completed to obtain a functional synergistic component.

[0054] Example 10: A method for preparing a natural plant essential oil compound with antibacterial efficacy is as follows:

[0055] S1: Add 100 g of the antibacterial active component to 700 g of the base oil phase, heat to 30°C, stir for 15 min at 900 rpm, add 120 g of the functional enhancement component, heat to 35°C, place in a homogenizer, and emulsify for 15 min to obtain an emulsion;

[0056] S2: 920 g of the emulsion was added to a vacuum degassing tank, the pressure was -0.08 MPa, degassed for 18 minutes, filled with nitrogen, cooled to 5°C, and matured for 22 hours to obtain a natural plant essential oil compound with antibacterial efficacy.

[0057] Example 11: A method for preparing a natural plant essential oil compound with antibacterial efficacy is as follows:

[0058] S1: Add 110 g of the antibacterial active component to 750 g of the base oil phase, heat to 33°C, stir for 13 min at 950 rpm, add 130 g of the functional enhancement component, heat to 37°C, place in a homogenizer, and emulsify for 13 min to obtain an emulsion;

[0059] S2: 990 g of the emulsion was added to a vacuum degassing tank, the pressure was -0.08 MPa, degassed for 20 min, filled with nitrogen, cooled to 3°C, and matured for 24 h to obtain a natural plant essential oil compound with antibacterial efficacy.

[0060] Example 12: A method for preparing a natural plant essential oil compound with antibacterial efficacy is as follows:

[0061] S1: Add 120 g of the antibacterial active component to 800 g of the base oil phase, heat to 35°C, stir for 10 min at 1000 rpm, add 140 g of the functional enhancement component, heat to 35°C, place in a homogenizer, and emulsify for 15 min to obtain an emulsion;

[0062] S2: 1060 g of the emulsion was added to a vacuum degassing tank, the pressure was -0.08 MPa, degassed for 22 min, filled with nitrogen, cooled to 0°C, and matured for 26 h to obtain a natural plant essential oil compound with antibacterial efficacy.

[0063] Comparative Example 1:

[0064] Compared with Example 10, this comparative example did not add antibacterial active components during the preparation process of the natural plant essential oil compound with antibacterial efficacy. The remaining steps and parameters were the same and will not be repeated in this comparative example. Finally, a natural plant essential oil compound was obtained.

[0065] Comparative Example 2:

[0066] Compared with Example 10, in the preparation process of the natural plant essential oil compound with only antibacterial efficacy in this comparative example, vacuum degassing and low-temperature maturation are omitted, and the emulsion is directly filled. The remaining steps and parameters are the same and will not be repeated in this comparative example. Finally, a natural plant essential oil compound is obtained.

[0067] Comparative Example 3:

[0068] Compared with Example 10, this comparative example only replaces the mass ratio of oregano essential oil, thyme essential oil, tea tree essential oil, eucalyptus essential oil and lavender essential oil of "1:3:9:1:5" with "1:5:15:3:8", and the remaining steps and parameters are the same. This comparative example will not be repeated, and finally a natural plant essential oil composite is obtained.

[0069] Comparative Example 4:

[0070] Compared with Example 10, this comparative example only replaces the "base oil phase" with "olive oil and grape seed oil", and the remaining steps and parameters are the same. This comparative example will not be repeated, and finally a natural plant essential oil compound is obtained.

[0071] Comparative Example 5:

[0072] Compared with Example 10, this comparative example only did not use ultrasonic treatment in the preparation of the functional enhancement component, only used a rotation speed of 500 rpm and stirred for 10 minutes, and the remaining steps and parameters were the same. This comparative example will not be repeated, and finally obtained a natural plant essential oil compound.

[0073] Performance testing:

[0074] Antibacterial performance test

[0075] Refer to ISO-22196 test standard, using Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC6538);

[0076] Take 10 g of each of the natural plant essential oil compound of Examples 10-12 and Comparative Examples 1-5, and spread it on a 50 mm×50 mm sterile membrane.

[0077] Take 1×10 6 Evenly spread 100 μL of CFU / mL bacterial solution on the sample surface, cover with sterile polyethylene film, and incubate at 37°C for 24 hours:

[0078] The sample was immersed in 10 mL of PBS buffer and ultrasonically vibrated for 5 min at a frequency of 40 kHz. After gradient dilution, it was spread on agar plates and incubated at 37°C for 24 h to count the number of colonies.

[0079] Calculation of antibacterial rate:

[0080]

[0081] Table 1

[0082] project The number of colonies in the blank control group (CFU / mL) Colony count in experimental group (CFU / mL) Antibacterial rate (%) Example 10 <![CDATA[1.5×10 6 ]]> <![CDATA[2.2×10 4 ]]> 98.5 Example 11 <![CDATA[1.5×10 6 ]]> <![CDATA[3.1×10 4 ]]> 97.9 Example 12 <![CDATA[1.5×10 6 ]]> <![CDATA[5.6×10 4 ]]> 96.3 Comparative Example 1 <![CDATA[1.5×10 6 ]]> <![CDATA[1.1×10 6 ]]> 25.4 Comparative Example 2 <![CDATA[1.5×10 6 ]]> <![CDATA[4.3×10 5 ]]> 71.6 Comparative Example 3 <![CDATA[1.5×10 6 ]]> <![CDATA[7.7×10 5 ]]> 48.9 Comparative Example 4 <![CDATA[1.5×10 6 ]]> <![CDATA[9.7×10 5 ]]> 35.2 Comparative Example 5 <![CDATA[1.5×10 6 ]]> <![CDATA[2.7×10 5 ]]> 82.1

[0083] Stability testing

[0084] The natural plant essential oil composites of Examples 10-12 and Comparative Examples 1-5, 20 g each, were placed in a constant temperature box at 25 ° C and 40 ° C, stored for 30 days, and visually inspected for stratification, precipitation or turbidity, and recorded the time and degree of occurrence. The centrifuge was used at a speed of 3000 rpm for 10 min to detect the mass ratio of the precipitate.

[0085] Table 2

[0086]

[0087]

[0088] Skin irritation test

[0089] According to the OECD 439 standard test, RhE was used to reconstruct the human epidermis model;

[0090] The RhE-reconstructed human epidermal model was equilibrated at 37°C and 5% CO2 for 24 hours. 100 μL of the natural plant essential oil mixtures of Examples 10-12 and Comparative Examples 1-5 were evenly applied to the model surface and exposed for 15 minutes. A blank control group was set up, and the samples were washed with PBS buffer and incubated for another 42 hours.

[0091] Add 0.3 mg / mL MTT reagent and incubate for 3 hours. Dissolve the formazan crystals and measure the absorbance at 570 nm. If the cell survival rate is less than 50%, it is considered "irritating". The cell survival rate is calculated as follows:

[0092]

[0093] Volatile component retention rate

[0094] The natural plant essential oil compound of Examples 10-12 and Comparative Examples 1-5, and the unmixed antibacterial active component raw materials of Examples 10-12 and Comparative Examples 1-5, 1.0 g each, were taken and analyzed by GC-MS. The chromatography column was DB-5MS (30 m × 0.25 mm × 0.25 μm), the injection volume was 1 μL, the split ratio was 10:1, the initial temperature was 50 ° C, the heating rate was 10 ° C / min, and the endpoint temperature was 250 ° C; the ion source was EI, 70 eV, and the mass spectrometry scanning range was 35-450 m / z;

[0095] Loss rate calculation formula:

[0096]

[0097] Table 3

[0098]

[0099]

[0100] Penetration testing

[0101] Sample preparation: evenly apply the essential oil compound to be tested on the surface of the transdermal membrane (the dosage is usually 2-5 mg / cm 2 )

[0102] Pig ear skin was taken, subcutaneous fat was removed, and the thickness was controlled to 200-400 μm. The skin was then immersed in PBS for 30 minutes to obtain a transdermal membrane. The transdermal membrane was then fixed between the donor pool and the receptor pool. The receiving medium was normal saline.

[0103] Take 10 g of each of the natural plant essential oil compounds of Examples 10-12 and Comparative Examples 1-5, and evenly apply them to the donor side of the transdermal membrane. Heat to 32 ± 1 ° C, rotate at 300 rpm, and continue for 24 hours. At the preset time points of 0.5 h, 1 h, 2 h, 4 h, 6 h, 8 h, and 24 h, sample 1 mL from the receptor pool and replenish with an equal amount of fresh receiving medium.

[0104] The concentration of active ingredient in the receiving medium was determined using a UV-Vis spectrophotometer, and the cumulative transdermal amount was calculated: Q = cumulative concentration × receiving cell volume / transdermal area, and the steady-state transdermal rate was calculated: Jss = slope / transdermal area, lag time, Tlag.

[0105] Table 4

[0106]

[0107]

[0108] Oxidation stability test

[0109] Take 5.0 g of each of the natural plant essential oil composites of Examples 10-12 and Comparative Examples 1-5, evenly load them into a reaction tube, place the sample tube in a heating module, connect the air duct to the conductive cell, start the device, continue to let air in and heat, monitor the change in conductivity, and when the conductivity significantly increases (indicating volatile acid generation), record the time as the oxidation induction period (OIT);

[0110] Shelf life test

[0111] Refer to ISO 4833-1:2013 test standard

[0112] 10 mL of each of the natural plant essential oil compounds of Examples 10-12 and Comparative Examples 1-5 were placed in a constant temperature and humidity chamber, and samples were taken for testing at 0, 1, 3, and 6 months.

[0113] Take 1 g of sample and add it to 9 mL of sterile saline, and dilute it to 10-3. Take 1 mL of the dilution and add it to PCA medium, incubate at 37℃ for 48 hours, and count the colonies (CFU / g).

[0114] Moisturizing performance test

[0115] The natural plant essential oil mixtures of Examples 10-12 and Comparative Examples 1-5 were evenly applied to the inner forearm of the test subject, in an area of 2 cm × 2 cm, and the temperature was raised to 22 ± 2 ° C and the humidity was 50 ± 5%;

[0116] The subjects cleaned the test area and let it dry for 30 minutes to ensure that the skin was in a natural state. CM825 measured the initial stratum corneum water content of the three groups of areas;

[0117] Calculation formula:

[0118] Moisturizing rate increase (%) = (water content after application (AU) - baseline water content (AU) / (baseline water content AU × 100

[0119] Table 5

[0120]

[0121]

[0122] Data Analysis:

[0123] As can be seen from Tables 1-5, the natural plant essential oil compound prepared by the present invention has significant advantages in antibacterial performance, stability, safety and functionality;

[0124] However, since no antibacterial active components were added to Comparative Example 1, it lost its core antibacterial function and lacked synergistic enhancement, resulting in the inability to stably release the moisturizing factor and accelerating stratification and precipitation. The reason is that the antibacterial active components contain polyphenols such as terpinene-4-ol and linalool and aldehyde compounds such as cinnamaldehyde, which can penetrate the bacterial cell membrane and make the bacterial ATP and K + At the same time, thymol can block DNA replication by binding to bacterial DNA gyrase, and the synergistic effect of tea tree essential oil and lavender essential oil can broaden the antibacterial spectrum to drug-resistant bacteria. In addition, tea tree essential oil and lavender essential oil contain α-terpineol, which forms a hydrogen bond network with the polysaccharides and saponins in the marigold soaked oil in the base oil phase, locking in moisture and improving its moisturizing properties. In addition, α-pinene in the antibacterial active component can reduce the oil-water interfacial tension and promote the formation of microemulsion droplets, thereby improving its stability.

[0125] Comparative Example 2 lacked vacuum degassing and low-temperature aging, resulting in residual bubbles in the emulsion, accelerating oxidative deterioration. Insufficient low-temperature aging resulted in inadequate molecular self-assembly, a loose network structure, and an inability to resist phase separation at high temperatures. This was because residual oxygen and tiny bubbles in the un-degassed emulsion increased the contact area between oxygen and active ingredients such as vitamin E and essential oils, initiating a free radical chain reaction, leading to lipid oxidation and ingredient degradation. Simultaneously, low-temperature aging promoted the orderly arrangement of intermolecular hydrogen bonds and van der Waals forces through slow cooling, forming a stable three-dimensional network structure and thus improving its stability. Furthermore, the lack of degassing resulted in residual oxygen, accelerating the oxidative inactivation of carvacrol, an antimicrobial active component. The lack of low-temperature aging prevented the active molecules from being fully embedded in the network structure, making them more volatile. Furthermore, stratification resulted in excessively low local concentrations of the antimicrobial components, making them ineffective in inhibiting microorganisms. This reduced the antimicrobial activity of Comparative Example 2.

[0126] Comparative Example 3: Since the ratio of the antibacterial active components exceeds the scope of the claims, the oregano essential oil ratio is too high, causing skin irritation, and the excessive tea tree essential oil causes the volatile components to escape during stirring, while destroying the synergistic antibacterial effect between the components. The reason is that the high concentration of tea tree essential oil inhibits the soothing effect of lavender essential oil and produces a competitive antibacterial mechanism with oregano essential oil, thereby reducing the overall efficiency and affecting the adsorption and penetration of active molecules on the biofilm, thereby destroying the synergistic effect between the antibacterial active components. In addition, the amount of tea tree essential oil is increased, and its highly volatile component terpinene-4-ol will escape more aggravated during stirring and standing, resulting in its antibacterial decrease. At the same time, carvacrol in oregano essential oil and terpinene-4-ol in tea tree essential oil are both highly irritating, which are mainly neutralized by the lavender essential oil and sweet almond oil base. If the ratio is unbalanced, and during the volatilization process, the thymol of the phenolic compound that is not completely lost remains in the finished product, which will directly damage the epidermal cells, resulting in increased irritation.

[0127] In Comparative Example 4, since the base oil phase is replaced with olive oil and grape seed oil, the active ingredient has poor permeability and cannot achieve long-term antibacterial effect. The reason is that the calendula soaked oil is rich in natural moisturizing factors such as polysaccharides and saponins, which can directly enhance the water-locking ability of the stratum corneum. Although olive oil and grape seed oil contain unsaturated fatty acids such as oleic acid and linoleic acid, they lack the hydrophilic moisturizing ingredients unique to calendula, resulting in a significant reduction in the moisturizing synergistic effect. At the same time, olive oil and grape seed oil have low polarity and poor compatibility with functional synergistic components, resulting in uneven distribution of moisturizing factors and inability to form a stable hydration film. In addition, α-bisabolol in the calendula soaked oil can promote the penetration of antibacterial active components into the skin. Deep, prolonging the antibacterial time, while olive oil and grape seed oil have higher viscosity, and the difference in polarity causes the antibacterial ingredients to be retained in the surface layer of the oil phase and unable to effectively contact microorganisms, resulting in insufficient antibacterial persistence. In addition, the saponins in calendula-infused oil can temporarily change the lipid arrangement of the stratum corneum and enhance the transdermal absorption of active ingredients, while olive oil and grape seed oil lack such penetration enhancers, resulting in the inability of functional synergistic components to effectively penetrate the skin barrier. At the same time, the combination of sweet almond oil and coconut oil can form a gradient polarity system, promoting the gradual release of active ingredients, while olive oil and grape seed oil have similar polarity, forming a uniform oil phase, hindering the diffusion of active ingredients and reducing their transdermal rate;

[0128] In Comparative Example 5, since the functional enhancement components were not dispersed ultrasonically, the functional enhancement components agglomerated and the active ingredients were unevenly distributed, affecting the permeability and uniformity of the emulsion. The reason is that the polarity difference between borneol oil and frankincense oil is large, and the shear force provided by ordinary stirring is insufficient to overcome the interfacial tension between the two phases, resulting in the inability to mix the two uniformly. At the same time, ultrasonic dispersion generates cavitation bubbles through high-frequency vibration, releasing instantaneous high pressure and high temperature, which can effectively break up the droplets and achieve nanoscale dispersion. Comparative Example 5 relies only on mechanical stirring, which has insufficient energy density and cannot achieve the same effect, resulting in component agglomeration. In addition, the agglomerates of borneol oil and frankincense oil form a physical barrier, hindering the free diffusion of vitamin E molecules, resulting in their uneven distribution in the matrix. Vitamin E needs to work synergistically with borneol oil and frankincense oil, but the uneven distribution reduces the contact area of the three, weakening the synergistic effect. In addition, the pore size of the skin stratum corneum is about 0.4-3.6 μm, and particles with D90>5 μm are difficult to pass through pores or intercellular spaces, so only a small amount of active ingredients can penetrate, resulting in a reduced transdermal rate.

[0129] Those skilled in the art should understand that the discussion of any of the above embodiments is merely illustrative and is not intended to imply that the scope of the present invention is limited to these examples. Within the scope of the present invention, the technical features in the above embodiments or different embodiments may be combined, the steps may be implemented in any order, and there are many other variations of the different aspects of the present invention as described above, which are not provided in detail for the sake of simplicity.

[0130] The present invention is intended to cover all such substitutions, modifications and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A natural plant essential oil compound with antibacterial efficacy, characterized in that: The invention is composed of the following components in parts by mass: 70-80 parts of base oil phase, 10-12 parts of antibacterial active components and 12-14 parts of functional enhancement components.

2. A natural plant essential oil compound with antibacterial efficacy according to claim 1, characterized in that: The base oil phase is prepared from sweet almond oil, jojoba oil, coconut oil and calendula infused oil.

3. A natural plant essential oil compound with antibacterial efficacy according to claim 2, characterized in that: The base oil phase preparation steps are as follows: Place sweet almond oil, jojoba oil and coconut oil in a stainless steel reactor, heat to 35-40°C, stir for 8-12 minutes at a speed of 150-250 rpm, add calendula infused oil, stir for 3-5 minutes, filter with a 5 μm filter membrane to remove impurities, and cool to 20-30°C to obtain a base oil phase.

4. A natural plant essential oil compound with antibacterial efficacy according to claim 3, characterized in that: The mass ratio of the sweet almond oil, jojoba oil, coconut oil and calendula infused oil is 9-10:2-3:1-2:

1.

5. A natural plant essential oil compound with antibacterial efficacy according to claim 1, characterized in that: The antibacterial active component is prepared from tea tree essential oil, lavender essential oil, thyme essential oil, oregano essential oil and eucalyptus essential oil.

6. The natural plant essential oil compound with antibacterial efficacy according to claim 5, characterized in that: The steps for preparing the antibacterial active component are as follows: Add oregano essential oil to a beaker, heat to 20-30°C, add thyme essential oil, tea tree essential oil, eucalyptus essential oil and lavender essential oil, stir for 8-10 minutes, rotate at 400-600 rpm, protect from light, and let stand for 10-12 hours to obtain antibacterial active components.

7. The natural plant essential oil compound with antibacterial efficacy according to claim 6, characterized in that: The mass ratio of the oregano essential oil, thyme essential oil, tea tree essential oil, eucalyptus essential oil and lavender essential oil is 1:3-4:9-10:1-2:5-6.

8. The natural plant essential oil compound with antibacterial efficacy according to claim 1, characterized in that: The functional synergistic component is prepared from borneol oil, frankincense oil, frankincense oil, bergamot essential oil, palmarosa oil and vitamin E oil.

9. The natural plant essential oil compound with antibacterial efficacy according to claim 8, characterized in that: The steps for preparing the functional enhancement component are as follows: Step A1: Add frankincense oil to borneol oil, raise the temperature to 25-30° C., and ultrasonicate for 3-5 minutes at a frequency of 30-40 Hz to obtain a mixed solution; Step A2: Add geranium essential oil, bergamot essential oil and palmarosa oil to the mixed solution, heat to 20-30°C, stir for 8-10 minutes, rotate at 700-800 rpm, then add vitamin E oil, stir for 3-5 minutes, and complete the reaction to obtain a functional enhancement component.

10. The natural plant essential oil compound with antibacterial efficacy according to claim 9, characterized in that: The mass ratio of frankincense oil to borneol oil in step A1 is 1:0.9-1.

1.

11. The natural plant essential oil compound with antibacterial efficacy according to claim 9, characterized in that: The mass ratio of geranium essential oil, bergamot essential oil, palmarosa oil, mixed solution and vitamin E oil described in step A2 is 0.8-1.2:1:0.8-1.2:2-3:0.6-0.

8.

12. A method for preparing a natural plant essential oil compound with antibacterial efficacy according to any one of claims 1 to 11, characterized in that: The preparation method is as follows: Step S1: adding the antibacterial active component to the base oil phase, heating to 30-35°C, stirring for 10-15 minutes at a speed of 900-1000 rpm, adding the functional enhancement component, heating to 35-40°C, placing in a homogenizer, and emulsifying for 10-15 minutes to obtain an emulsion; Step S2: adding the emulsion into a vacuum degassing tank, at a pressure of -0.08 MPa, degassing for 18-22 minutes, filling with nitrogen, cooling to 0-5°C, and aging for 22-26 hours to obtain a natural plant essential oil compound with antibacterial efficacy.

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