Anti-hair loss composition containing cacumen biotae extract and preparation method of anti-hair loss composition

Abstract

The invention discloses an anti-hair-loss composition containing a cacumen biotae extract and a preparation method of the anti-hair-loss composition, and particularly belongs to the technical field of scalp care and raw material compounding. The anti-hair loss composition comprises the following components: 8-12 parts of a red ginseng extract, 8-12 parts of a Chinese arborvitae twig and leaf extract, 3-4 parts of a flat ground mushroom extract, 2-3 parts of a dendrobium officinale stem extract and 1-2 parts of lactobacillus / ginseng root fermentation product filtrate. The anti-alopecia composition provided by the invention has a remarkable effect of inhibiting the activity of 5alpha-reductase, can remarkably improve the alopecia phenomenon of mice, inhibits the generation of testosterone and dihydrotestosterone, and reduces the risk of androgen-derived alopecia.

Description

Technical Field

[0001] The present invention belongs to the technical field of scalp care and raw material compounding, and specifically relates to an anti-hair loss composition containing Platycladus orientalis leaf extract and a preparation method thereof. Background Art

[0002] Androgenic alopecia (AGA), also known as seborrheic alopecia, is one of the most common types of hair loss in clinical practice. Its main pathological features include an oily scalp, a receding hairline in an "M" shape in men, an "O"-shaped hair loss on the top of the head, or a "U"-shaped pattern of both. In women, hair thinning, fine hair, and a widening of the parting of the hairline are common. Hair loss can cause significant psychological stress for patients, impacting their work and personal lives.

[0003] Studies have shown that patients with seborrheic alopecia have elevated levels of dihydrotestosterone (DHT) in their blood, and microscopically exhibit hair follicle atrophy. 5α-Reductase (5αR) is a key enzyme in the metabolism of the androgen testosterone (T). With the electrons provided by reduced coenzyme II (NADPH), it reduces the unsaturated double bonds at positions 4 and 5 of testosterone, converting it to DHT. Overexpression of 5αR produces high levels of DHT, which has a five-fold greater ability to bind to the androgen receptor (AR) than T. High levels of DHT binding to the AR can lead to androgen-dependent diseases such as androgenic alopecia, prostatic hyperplasia, acne, and hirsutism. 5αR is divided into three types. Type I 5α-reductase (5αR1) is mainly distributed in organs such as the liver, sebaceous glands, and sweat glands, with an optimal pH of 6 to 9; type II 5α-reductase (5αR2) is mainly present in organs such as the epididymis, prostate, and scalp hair follicles, with an optimal pH of 5.5; type III 5α-reductase (5αR3) is almost not expressed or rarely expressed in normal organs, but is overexpressed in prostate cancer.

[0004] Currently, reducing DHT levels by inhibiting 5αR1 and 5αR2 has become an important method for treating androgenic alopecia. Drugs based on this method include finasteride, dutasteride, and aprelimin, but these synthetic 5αR inhibitors have certain side effects and are relatively expensive. Finding 5αR inhibitors from natural plants and microbial products has become a new approach. Summary of the Invention

[0005] In view of the shortcomings of the existing technology, the purpose of the present invention is to provide an anti-hair loss composition composed of a variety of natural extracts, which has the effect of significantly inhibiting the activity of 5α-reductase, thereby reducing the conversion of DHT and reducing the risk of androgenic alopecia.

[0006] In order to achieve the above object, the present invention discloses the following scheme:

[0007] In a first aspect, the present invention provides an anti-hair loss composition, which comprises the following components in parts by mass:

[0008]

[0009] Preferably, the method for preparing the lactobacillus / ginseng root fermentation product filtrate comprises the following steps:

[0010] 1-1. Take ginseng root slices and bake to constant weight to obtain dried ginseng root slices, which are then crushed through a ≥60 mesh sieve to obtain ginseng root powder;

[0011] 1-2. Ginseng root powder was mixed with deionized water at a mass volume ratio of 1:10 g / mL, and a composite enzyme preparation of 4-5% of the total mass of the liquid was added. The enzymatic hydrolysis was carried out at 45-55 ° C for 14-16h. The composite enzyme preparation contains protease, cellulase and amylase. After the enzymatic hydrolysis is completed, the enzyme was heated to 90-100 ° C for 15 min to inactivate the enzyme to obtain an enzymatic solution;

[0012] 1-3. Add nitrogen source, carbon source, inorganic salt and defoamer to the enzymatic hydrolysate to obtain fermentation medium, sterilize at 121°C for 15-20 min and set aside;

[0013] 1-4. Take the number of viable bacteria 1-2×10 8 A Lactobacillus acidophilus liquid with a CFU / mL was inoculated into a fermentation medium at a rate of 10-15 v / v%, and fermented for 24-36 hours at 35-37°C, a rotation speed of 100-150 r / min, and a dissolved oxygen content of 0.5-1% to obtain a fermentation liquid;

[0014] 1-5. The fermentation broth was centrifuged at 10,000 rpm for 15-20 min, and the obtained supernatant was sterilized by filtration through a 0.22 μm filter membrane to obtain a lactobacillus / ginseng root fermentation product filtrate.

[0015] More preferably, in step 1-1, the sliced ginseng roots are placed at 50-60° C. for drying.

[0016] Further preferably, the protease activity in the complex enzyme preparation is ≥500 U / g, the cellulase activity is ≥1200 U / g, and the amylase activity is ≥500 U / g.

[0017] Further preferably, the fermentation medium is obtained by adding 15g glucose, 10g peptone, 5g yeast extract, 5g beef extract, 2g K2HPO4, 5g sodium acetate, 2g triammonium citrate, 0.2g MgSO4·7H2O, 0.05g MnSO4·H2O, and 1mL Tween 80 to each kilogram of enzymatic hydrolyzate.

[0018] In a second aspect, the present invention provides a use of the anti-hair loss composition described in the first aspect in the preparation of a hair care product with the efficacy of preventing hair loss and promoting hair growth.

[0019] In a third aspect, the present invention provides a scalp care solution for preventing hair loss, wherein the scalp care solution comprises the anti-hair loss composition according to the first aspect;

[0020] The amount of the anti-hair loss composition added to the scalp care solution is 1-10 wt %.

[0021] Preferably, the scalp care solution further comprises a moisturizer, a film former, an emollient, a scalp conditioner, a pH regulator, a preservative, and a solvent.

[0022] Further preferably, the scalp conditioning agent is selected from at least one of raspberry extract, ginger root extract, clover extract, polygonum multiflorum root extract, emblica fruit extract, and artemisia argyi extract.

[0023] In a fourth aspect, the present invention provides a method for preparing the scalp care solution according to the third aspect, characterized in that the preparation method comprises the following steps:

[0024] The anti-hair loss composition, moisturizing agent, film-forming agent, emollient, scalp conditioner, pH regulator, preservative and solvent are stirred and mixed uniformly to obtain an anti-hair loss scalp care liquid.

[0025] In the present invention:

[0026] Red ginseng extract is rich in ginsenosides, polyphenols and polysaccharides. It reduces DHT production by inhibiting 5α-reductase activity (especially the 5αR2 subtype), while activating the Wnt / β-catenin pathway to promote the proliferation of dermal papilla cells. Its antioxidant properties can protect hair follicles from free radical damage, thereby delaying the process of hair loss and enhancing hair resilience.

[0027] Platycladus orientalis leaf extract contains active ingredients such as flavonoids such as quercetin and kaempferol, as well as volatile oils, which have significant anti-inflammatory and antibacterial effects. They can inhibit the reproduction of scalp pathogens and reduce the release of inflammatory factors. At the same time, it can also promote blood circulation in the scalp, dilate capillaries, increase blood oxygen supply to hair follicles, prolong the growth period of hair follicles, and improve the symptoms of seborrheic alopecia.

[0028] The extract of Agaricus rutaecarpa is rich in polysaccharides, proteins, fatty acids, amino acids and other bioactive ingredients, which can effectively promote the proliferation of hair follicle cells and improve the vitality of hair follicle cells.

[0029] Dendrobium officinale stem extract contains dendrobium polysaccharides, dendrobium alkaloids and amino acid groups, which can slightly improve the energy metabolism and keratin synthesis efficiency of hair papilla cells. At the same time, it can repair the scalp barrier and maintain a weakly acidic environment, thereby improving hair loss.

[0030] After ginseng root is fermented and transformed by Lactobacillus acidophilus, the bioavailability of saponins is improved, and 5α-reductase is effectively inhibited. The short-chain fatty acids in the fermentation product filtrate can effectively regulate the balance of scalp flora, and antimicrobial peptides can target and inhibit inflammatory pathways, synergistically reduce inflammatory damage to hair follicles and promote a healthy hair growth microenvironment.

[0031] The present invention has found that adding Agaricus rutaecarpa extract, Dendrobium officinale stem extract and Lactobacillus / ginseng root fermentation product filtrate to a formula system mainly composed of red ginseng extract and Platycladus orientalis leaf extract can effectively improve the effect of the system in inhibiting 5α-reductase and promote the efficacy of the overall formula.

[0032] Dendrobium officinale stem extract contains

[0033] Beneficial effects of the present invention:

[0034] 1. The anti-hair loss composition provided by the present invention, comprising red ginseng extract, Platycladus orientalis leaf extract, Agaricus rutaecarpa extract, Dendrobium officinale stem extract, and Lactobacillus / ginseng root fermentation product filtrate, has significant efficacy in inhibiting 5α-reductase activity. Experimental studies have demonstrated that Agaricus rutaecarpa extract, Dendrobium officinale stem extract, and Lactobacillus / ginseng root fermentation product filtrate promote and enhance the efficacy of the anti-hair loss formulation system comprised of red ginseng extract and Platycladus orientalis leaf extract.

[0035] 2. The red ginseng extract, Platycladus orientalis leaf extract, Agaricus rutaecarpa extract, Dendrobium officinale stem extract, and Lactobacillus / ginseng root fermentation product filtrate provided in this application can significantly improve hair loss in mice when used in combination, inhibit the production of testosterone and dihydrotestosterone, and reduce the risk of androgenic alopecia. DETAILED DESCRIPTION

[0036] Those skilled in the art can refer to the contents of this article and appropriately improve the process parameters. It should be noted that all similar replacements and modifications are obvious to those skilled in the art and are considered to be included in the present invention.

[0037] The products and methods of the present invention have been described through preferred embodiments. It is obvious that relevant personnel can modify or appropriately change and combine the products and methods described herein without departing from the content, spirit and scope of the present invention to implement and apply the technology of the present invention.

[0038] In the present invention:

[0039] The experimental methods in the following examples where specific conditions are not specified are generally carried out under conventional conditions or conditions recommended by the manufacturers.

[0040] Unless otherwise specified, all materials and reagents used in this invention are commercially available. Unless otherwise specified, the materials described in this invention are calculated by mass percentage and mass fraction.

[0041] Ginseng root: the root of the ginseng plant (Panax ginseng CAMey.);

[0042] Lactobacillus acidophilus was purchased from China General Microorganism Culture Collection Center, with the collection number of CGMCC 1.1854.

[0043] Preparation of Lactobacillus / Ginseng Root Fermentation Filtrate

[0044] Step 1-1. Take fresh ginseng root slices, place them in an oven at 50-60 ° C and bake them to constant weight to obtain dried ginseng root slices, and then place them in a high-speed grinder and grind them through a 60-mesh sieve to obtain ginseng root powder;

[0045] Step 1-2. Ginseng root powder and deionized water were mixed at a mass-to-volume ratio of 1:10 g / mL, and a composite enzyme preparation of 4.5% of the total mass of the liquid was added. Enzyme hydrolysis was performed at 49°C for 15 hours. The composite enzyme preparation contained protease, cellulase, and amylase, with protease activity ≥500 U / g, cellulase activity ≥1200 U / g, and amylase ≥500 U / g. After the enzymatic hydrolysis was completed, the enzyme was heated to 96°C for 15 minutes to inactivate the enzyme to obtain an enzymatic solution;

[0046] Steps 1-3. To each kilogram of enzymatic hydrolysate, add 15 g glucose, 10 g peptone, 5 g yeast extract, 5 g beef extract, 2 g K2HPO4, 5 g sodium acetate, 2 g triammonium citrate, 0.2 g MgSO4·7H2O, 0.05 g MnSO4·H2O, and 1 mL Tween 80 to obtain a fermentation medium. Sterilize at 121°C for 15 min until ready for use.

[0047] Step 1-4: Take the number of viable cells 1-2×10 8A Lactobacillus acidophilus liquid with a CFU / mL inoculation rate of 13 v / v% (calculated on the fermentation medium) was inoculated into the fermentation medium obtained in steps 1-3, and the culture was carried out at 37° C., a rotation speed of 150 r / min, and a dissolved oxygen content of 0.8% for 36 hours. The obtained fermentation liquid was centrifuged at 10,000 r / min for 15 minutes, and the obtained supernatant was filtered and sterilized through a 0.22 μm filter membrane to obtain a lactobacillus / ginseng root fermentation product filtrate.

[0048] Preparation of anti-hair loss composition

[0049] Accurately weigh the raw materials in Table 1 according to the mass ratio, then place them in a container and mix and stir them evenly to obtain an anti-hair loss composition;

[0050] Table 1 Mass ratio of each raw material in the anti-hair loss composition

[0051]

[0052] Note: “-” in the table means no additive.

[0053] Performance Testing

[0054] 1 5α-reductase (5αR) inhibition experiment

[0055] 1.1 Preparation of 5αR Extract

[0056] (1) Pretreatment: Four male SD rats (purchased from Guangdong Medical Laboratory Animal Center, animal license number SCXK (Yue) 2022-0002) were fasted for 24 hours before the experiment and killed by cervical dislocation;

[0057] (2) Liver lavage: Pre-cooled saline (4°C) is perfused through the portal vein until the liver tissue becomes uniformly pale yellow;

[0058] (3) Tissue processing: The whole liver tissue was quickly removed and the wet weight was measured using a precision electronic balance. Pre-cooled pH 6.5 Tris buffer was added at a tissue mass to buffer volume ratio of 1:2 (g / mL);

[0059] (4) Low-temperature homogenization: Use a tissue homogenizer at 3000 rpm under ice bath conditions (3 cycles with ice bath cooling in between) to prepare liver tissue homogenate;

[0060] (5) Centrifugation purification: The homogenate was centrifuged at 10,000 × g for 30 min at 4°C, and the supernatant rich in 5αR was collected;

[0061] (6) Aliquot and store: Aliquot the clarified enzyme extract into sterile cryovials and immediately transfer to a -20°C freezer for storage.

[0062] 1.2 5αR activity assay

[0063] (1) Test samples: Compositions 1-8 prepared above were taken respectively, mixed with deionized water at a mass ratio of 1:11.5, and placed in a homogenizer for homogenization and uniform dispersion to obtain test samples 1-8 containing 8 wt% of the composition.

[0064] (2) Reaction system: 0.5 mL of phosphate buffer, 0.2 mL of the test sample, 200 μL of a 300 mg / L testosterone solution, and 200 μL of a 0.8 g / L NADPH (reduced coenzyme II) solution were sequentially added to a stoppered test tube, and finally 0.5 mL of the 5αR extract prepared above was added. The reaction was carried out at 37°C for 30 min.

[0065] (3) Control settings: Positive control: replace the test sample with an equal volume of 0.05 mg / L finasteride solution; enzyme reaction tube: contains only enzyme extract and substrate (no test sample); blank tube: no enzyme extract (replaced with an equal volume of buffer).

[0066] (4) Detection: After the reaction is completed, 3 mL of dichloromethane is added to stop the reaction and extract testosterone, and then 0.25 mL of 100 mg / L propyl hydroxybenzoate is added as an internal standard. Centrifuge at 5000 r / min for 10 min. Discard the upper aqueous phase, remove about 1 mL of the organic phase, evaporate to dryness, dissolve the residue in 1.5 mL of methanol, and take 10 μL of it to determine the residual testosterone content by high performance liquid chromatography. The high performance liquid chromatography conditions are: the chromatographic column is C 18 The column was 4.6×250 mm, 5 μm, the mobile phase was methanol / water (70:30, v / v), the flow rate was 1.0 mL / min, the detection wavelength was 254 nm, and the injection volume was 10 μL.

[0067] The 5αR inhibition rate was calculated according to the following formula:

[0068]

[0069] Where: W is the ratio of the testosterone peak area to the internal standard peak area.

[0070] 1.3 Test Results

[0071] Table 2 Inhibitory effect on 5αR

[0072] Group 5αR inhibition rate / % Positive control group 89.72 Test sample 1 (corresponding to composition 1) 57.89 Test sample 2 (corresponding to composition 2) 60.12 Test sample 3 (corresponding to composition 3) 59.34 Test sample 4 (corresponding to composition 4) 41.09 Test sample 5 (corresponding to composition 5) 43.98 Test sample 6 (corresponding to composition 6) 37.52 Test sample 7 (corresponding to composition 7) 34.87 Test sample 8 (corresponding to composition 8) 22.16

[0073] Result Analysis

[0074] According to the results in Table 2, compositions 1-3 have strong inhibitory abilities against 5α-reductase, with composition 2 being the best, with an inhibition rate of 60.12%. Although there is a certain gap compared to the positive control group, it still reaches 60% of the performance of the positive control group, indicating that the compositions provided by the present invention can reduce DHT and alleviate androgenic alopecia caused by androgens;

[0075] Compared with composition 2, compositions 4-6 lacked Agaricus rutaecarpa extract, Dendrobium officinale stem extract, and Lactobacillus / ginseng root fermentation product filtrate, respectively, resulting in an inferior inhibitory effect on 5αR compared to composition 2. This indicates that in the formula system composed of red ginseng extract and Platycladus orientalis leaf extract, Agaricus rutaecarpa extract, Dendrobium officinale stem extract, and Lactobacillus / ginseng root fermentation product filtrate can effectively enhance the inhibitory effect of the formula system on 5αR, and Agaricus rutaecarpa extract, Dendrobium officinale stem extract, and Lactobacillus / ginseng root fermentation product filtrate play a key role in the formula;

[0076] Compared with composition 2, composition 7 only retains red ginseng extract and Platycladus orientalis leaf extract. Although it has a certain 5αR inhibitory effect, it is still far behind composition 2. This once again proves that Agaricus rutaecarpa extract, Dendrobium officinale stem extract, and Lactobacillus / ginseng root fermentation product filtrate can effectively enhance the effect of this formula system in inhibiting 5αR.

[0077] 2 Experiment on prevention and treatment of hair loss in mice

[0078] 2.1 Group Processing

[0079] In the experiment, 110 SPF-grade C57BL / 6J male mice (purchased from Guangdong Medical Experimental Animal Center, animal license number SCXK (Yue) 2022-0002) weighing 20±2 g were selected and divided into 11 treatment groups using a completely randomized method, with 10 mice in each group (n=10): blank control group, model control group, positive drug group and experimental drug group (compositions 1-8).

[0080] 2.2 Modeling and experimental intervention plan are as follows:

[0081] Before the experiment, the modeling reagent was prepared: 25 mg / mL testosterone propionate injection was diluted with soybean oil for injection to obtain a 5 mg / mL testosterone propionate solution for use.

[0082] (1) Subcutaneous injection: The blank control group received a subcutaneous injection of soybean oil (the same volume as the modeling reagent in the modeling group) on the back every day, and the other groups received a 5 mg / (kg·d) testosterone propionate solution modeling reagent.

[0083] (2) Local administration: While maintaining the model, each group received drug intervention on the back hair area every day:

[0084] The positive drug group applied 0.3 mL of minoxidil tincture;

[0085] Each of the experimental groups was smeared with 0.3 mL of test solution 1-8 (a solution containing 8 wt % of the composition, the solvent was normal saline);

[0086] The blank control group and the model control group were smeared with an equal amount of normal saline.

[0087] To ensure drug absorption, each medication group was given the drug twice a day, in the morning and afternoon, with 0.15 mL applied each time. The above combined intervention of injection and medication continued for 10 weeks.

[0088] 2.3 Detection indicators and methods

[0089] (1) Quantitative analysis of hair loss: Hair samples were collected daily starting from the fifth week. A standardized hair collection process was used for each group. The hair on the back of each mouse was combed with the same comb at a fixed time period. The shed hair was collected with tweezers and weighed. The weight of the shed hair was accurately weighed to investigate the degree of hair loss in the mice.

[0090] (2) Hormone Level Detection: At the end of the experiment, blood was collected from the eye socket to obtain serum samples. The samples were centrifuged at 13,000 rpm for 10 min at 4°C, aliquoted, and stored at -80°C. ELISA was used to quantitatively detect serum testosterone, dihydrotestosterone, and estradiol levels. The procedures were strictly in accordance with the kit instructions.

[0091] 2.4 Statistical methods

[0092] SPSS19 statistical analysis system was used for data processing, and independent sample t-test was used to compare differences between groups, with P < 0.05 as the standard for judging statistically significant differences.

[0093] 2.5 Experimental Results

[0094] 2.5.1 Mouse hair removal quality

[0095] Table 2 Total weight of mice depilated from week 5 to week 10

[0096]

[0097]

[0098] Note: In the table, "a" indicates significant difference compared with the model control group, P < 0.05, and "b" indicates significant difference compared with the composition 2 group, P < 0.05.

[0099] 2.5.3 Mouse serum testosterone, dihydrotestosterone, and estradiol levels

[0100] Table 3 Serum testosterone, dihydrotestosterone and estradiol levels in mice

[0101]

[0102] Note: In the table, "a" indicates significant difference compared with the model control group, P < 0.05, and "b" indicates significant difference compared with the composition 2 group, P < 0.05.

[0103] 2.6 Results Analysis

[0104] Androgens are one of the important factors affecting hair growth. Studies have shown that an abnormal increase in androgens can lead to an imbalance in the ratio of testosterone, dihydrotestosterone, and estradiol, which in turn leads to androgenic alopecia. Through the research of this application, it was found that compositions 1-3 can effectively reduce the levels of testosterone and dihydrotestosterone in the body, which is related to their strong inhibition of 5α-reductase activity; experimental results show that the red ginseng extract, arborvitae leaf extract, flat mushroom extract, Dendrobium officinale stem extract, and lactobacillus / ginseng root fermentation product filtrate provided in this application can significantly improve the hair loss phenomenon of mice when used in combination, inhibit the production of testosterone and dihydrotestosterone, and reduce the risk of androgenic alopecia.

[0105] Preparation of scalp care solution for preventing hair loss

[0106] Accurately weigh the raw materials according to the mass percentage in Table 4;

[0107] Step 2-1. Take deionized water and slowly add sodium hyaluronate to it at 25°C and 200 rpm while stirring until it is completely dissolved. Then add glycerol and 1,2-hexanediol and continue stirring to mix evenly to obtain an aqueous phase.

[0108] Step 2-2. After heating the aqueous phase to 60°C, PEG-40 hydrogenated castor oil was added, stirred at 200 r / min and kept warm for 15 minutes to fully disperse it to obtain a stable mixed phase;

[0109] Step 2-3. After the mixed phase is cooled to 30°C, the scalp conditioner and anti-hair loss composition 2 are added in sequence, stirred at 300 rpm for 20 minutes in the dark, and then p-hydroxyacetophenone is added and stirred until completely dissolved;

[0110] Step 2-4. Adjust the pH to 5.7-6.2 with citric acid, then sterilize and filter through a 0.45 μm microporous filter membrane, and fill in a sterile environment to obtain an anti-hair loss scalp care solution.

[0111] Table 4 Hair care essence raw materials mass percentage

[0112]

[0113] Note: “-” in the table means no additive.

[0114] Although specific embodiments of the present invention have been described above, those skilled in the art will appreciate that these are merely illustrative and that the scope of the present invention is defined by the appended claims. Those skilled in the art may make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, and such changes and modifications are intended to fall within the scope of the present invention.

Claims

1. An anti-hair loss composition, characterized in that The composition comprises the following components in parts by mass: 8-12 parts of red ginseng extract; 8-12 parts of Platycladus orientalis leaf extract; 3-4 parts of flat mushroom extract; 2-3 parts of Dendrobium officinale stem extract; 1-2 parts Lactobacillus / Ginseng root ferment filtrate.

2. The composition according to claim 1, characterized in that The preparation method of the lactobacillus / ginseng root fermentation product filtrate comprises the following steps: 1-1. Take ginseng root slices and bake to constant weight to obtain dried ginseng root slices, which are then crushed through a ≥60 mesh sieve to obtain ginseng root powder; 1-2. Ginseng root powder was mixed with deionized water at a mass volume ratio of 1:10 g / mL, and a composite enzyme preparation of 4-5% of the total mass of the liquid was added. The enzymatic hydrolysis was carried out at 45-55 ° C for 14-16h. The composite enzyme preparation contains protease, cellulase and amylase. After the enzymatic hydrolysis is completed, the enzyme was heated to 90-100 ° C for 15 min to inactivate the enzyme to obtain an enzymatic solution; 1-3. Add nitrogen source, carbon source, inorganic salt and defoamer to the enzymatic hydrolysate to obtain fermentation medium, sterilize at 121°C for 15-20 min and set aside; 1-4. Take the number of viable bacteria 1-2×10 8 A Lactobacillus acidophilus liquid with a CFU / mL was inoculated into a fermentation medium at a rate of 10-15 v / v%, and fermented for 24-36 hours at 35-37°C, a rotation speed of 100-150 r / min, and a dissolved oxygen content of 0.5-1% to obtain a fermentation liquid; 1-5. The fermentation broth was centrifuged at 10,000 rpm for 15-20 min, and the obtained supernatant was sterilized by filtration through a 0.22 μm filter membrane to obtain a lactobacillus / ginseng root fermentation product filtrate.

3. The composition according to claim 2, characterized in that In the step 1-1, the sliced ginseng roots are placed at 50-60° C. for drying.

4. The composition according to claim 2, characterized in that The protease activity in the complex enzyme preparation is ≥500 U / g, the cellulase activity is ≥1200 U / g, and the amylase activity is ≥500 U / g.

5. The composition according to claim 2, characterized in that The fermentation medium was prepared by adding 15 g glucose, 10 g peptone, 5 g yeast extract, 5 g beef extract, 2 g K2HPO4, 5 g sodium acetate, 2 g triammonium citrate, 0.2 g MgSO4·7H2O, 0.05 g MnSO4·H2O, and 1 mL Tween 80 to each kilogram of enzymatic hydrolyzate.

6. Use of the anti-hair loss composition according to any one of claims 1 to 5 in the preparation of a hair care product having the efficacy of preventing hair loss and promoting hair growth.

7. A scalp care liquid for preventing hair loss, characterized in that: The scalp care solution comprises the anti-hair loss composition according to any one of claims 1 to 5; The amount of the anti-hair loss composition added to the scalp care solution is 1-10 wt %.

8. The scalp care solution according to claim 7, characterized in that: The scalp care solution also contains a moisturizer, a film-forming agent, an emollient, a scalp conditioner, a pH regulator, a preservative, and a solvent.

9. The scalp care solution according to claim 8, characterized in that: The scalp conditioning agent is selected from at least one of raspberry extract, ginger root extract, clover extract, polygonum multiflorum root extract, emblica fruit extract, and mugwort extract.

10. The method for preparing the scalp care solution according to claim 8 or 9, characterized in that: The preparation method comprises the following steps: The anti-hair loss composition, moisturizing agent, film-forming agent, emollient, scalp conditioner, pH regulator, preservative and solvent are stirred and mixed uniformly to obtain an anti-hair loss scalp care liquid.

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