Tea polyphenol composition with brightening and tightening effects and application thereof

Abstract

The invention discloses a tea polyphenol composition with brightening and tightening effects and application thereof, and belongs to the technical field of cosmetics. The tea polyphenol composition provided by the invention is prepared from the following components in parts by mass: 0.5 to 1 part of tea polyphenol, 0.1 to 0.3 part of oxyresveratrol and 1 to 3 parts of alpha-glucan oligosaccharide. According to the invention, by selecting the components with proper mass parts, the components cooperate with each other, the stability of the composition can be effectively improved, and the components cooperate with each other, so that short-term and long-term skin whitening, brightening and tightening effects can be better realized.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a tea polyphenol composition with brightening and firming effects and an application thereof. Background Art

[0002] Tea polyphenols is a general term for the polyhydroxyphenol compounds found in tea leaves. Its main components include catechins (60%-80%), flavonoids, and anthocyanins. Their free radical-scavenging capacity is dozens of times greater than that of vitamins C and E. They can delay skin aging, inhibit tyrosinase activity, reduce melanin production, and have anti-inflammatory and pore-tightening properties.

[0003] However, existing tea polyphenols face difficulties in cosmetic application. Tea polyphenols are stable at pH 4-8 and room temperature, but are easily decomposed when encountering metal components such as iron and copper. They are very likely to undergo complexation reactions, resulting in oxidation and yellowing, and the degree of yellowing is uncontrollable. At the same time, the formula of the cosmetic base liquid is highly selective, and existing cosmetics have difficulty maintaining the stability of the formula with added tea polyphenols. The longer the application time, the weaker the efficacy, that is, the compatibility of the tea polyphenols in the formula is poor, and it is difficult to exert its corresponding efficacy. Summary of the Invention

[0004] The object of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide a tea polyphenol composition and its application having good stability, as well as good short-term and long-term skin brightening and skin firming effects.

[0005] To achieve the above objectives, in a first aspect of the present invention, a tea polyphenol composition having brightening and firming effects is provided, wherein the tea polyphenol composition comprises the following components in parts by weight:

[0006] 0.5-1 parts of tea polyphenols, 0.1-0.3 parts of oxidized resveratrol, and 1-3 parts of α-glucan oligosaccharides.

[0007] The present invention can effectively improve the stability of the composition by selecting components in appropriate mass parts and cooperating with each other. The components also work together to better achieve short-term and long-term skin whitening, brightening and firming effects.

[0008] Specifically, tea polyphenols have a powerful ability to scavenge free radicals, which can effectively tighten the skin. At the same time, they can inhibit the activity of tyrosinase and reduce the production of melanin, thereby effectively brightening the skin; oxidized resveratrol is a natural diphenylethylene compound, and oxidized resveratrol is the oxidation product of resveratrol, which can inhibit the release of inflammatory mediators, reduce skin inflammatory response, and relieve sensitive skin and inflammatory skin problems; α-glucan oligosaccharides are oligosaccharides formed by glucose connected by α-glycosidic bonds, and the degree of polymerization is generally between 2-10. Compared with glucan, α-glucan oligosaccharides have excellent water solubility and can form a breathable moisturizing film on the skin surface to prevent water loss, keep the skin moist and soft, and have the advantages of promoting skin absorption and efficient penetration into the skin. The present invention has found that by selecting the appropriate mass proportions of the above three ingredients for compounding, the instability problem of tea polyphenols can be effectively solved, and the problem that the effects of oxidized resveratrol and α-glucan oligosaccharides are not as good as those of resveratrol and glucan can be avoided; after the three ingredients are compounded, not only can excellent skin brightening and firming effects be achieved in a relatively short period of time, but the effects are also long-lasting and stable.

[0009] As a preferred embodiment of the tea polyphenol composition of the present invention, the tea polyphenol composition comprises the following components in parts by mass: 0.7-0.8 parts of tea polyphenols, 0.15-0.25 parts of oxidized resveratrol, and 1.5-2.5 parts of α-glucan oligosaccharides.

[0010] The present invention has found that the mass parts of the components in the tea polyphenol composition will affect its comprehensive effect. When the mass parts of the components in the tea polyphenol composition are further selected to be within the above-mentioned range, when the obtained tea polyphenol composition is applied to subsequent cosmetics, the obtained cosmetics have better stability, better skin brightening and firming effects, and the above-mentioned effects are not only fast-acting but also long-lasting.

[0011] As a preferred embodiment of the tea polyphenol composition of the present invention, the tea polyphenol composition further comprises an antioxidant, and the antioxidant comprises at least one of vitamin C and vitamin E.

[0012] The present invention has found that further adding at least one of vitamin C and vitamin E as an antioxidant to the composition can not only further stabilize the tea polyphenol composition, but also better realize the good antioxidant properties of the tea polyphenol composition, thereby achieving an excellent skin brightening and firming effect.

[0013] As a preferred embodiment of the tea polyphenol composition of the present invention, the mass percentage of the antioxidant is 0.1-0.6% based on the mass of the tea polyphenol.

[0014] Illustratively, based on the mass of tea polyphenols, the mass percentage of the antioxidant can be any point value or any two point range values between 0.1-0.6%, such as 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, 0.55%, 0.6%, etc.

[0015] The present invention has found that when the mass percentage of the antioxidant is within the above range based on the mass of the tea polyphenols, the comprehensive effect of the obtained tea polyphenol composition is more excellent.

[0016] It should be noted that the oxidized resveratrol in the present invention can also be encapsulated in a nanoliposome structure. Specifically, the encapsulated layer of the nanoliposome structure comprises the following components in weight percentage: 3-5% lecithin, 15-25% butylene glycol, and 55-75% water. The nanocomposite material has a particle size of 200 nm to 300 nm. The weight percentage of oxidized resveratrol in the nanoliposome structure is 2-4%. Using oxidized resveratrol in a nanoliposome structure can improve the stability of the tea polyphenol composition and enhance its short-term and long-term skin brightening and firming effects.

[0017] In a second aspect of the present invention, the present invention provides use of the tea polyphenol composition in preparing cosmetics.

[0018] The tea polyphenol composition provided by the present invention has good free radical scavenging and antioxidant effects, and the provided tea polyphenol composition has excellent stability. Therefore, it can be widely used as an active ingredient in cosmetics to achieve excellent skin brightening and firming effects.

[0019] As a preferred embodiment of the application of the present invention, the cosmetics include any one of lotion, emulsion, essence, cream, and mask.

[0020] In a third aspect of the present invention, the present invention provides an emulsion, comprising the tea polyphenol composition of the present invention.

[0021] By adding the tea polyphenol composition of the present invention to the emulsion provided by the present invention, the purpose of brightening and firming the skin with fast onset and long-lasting effect can be effectively achieved.

[0022] As a preferred embodiment of the emulsion of the present invention, the mass percentage of the tea polyphenol composition is 1-5% based on the total mass of the emulsion.

[0023] Illustratively, based on the total mass of the emulsion, the mass percentage of the tea polyphenol composition can be any point value between 1-5% or any two points in the range, such as 1%, 2%, 3%, 4%, 5%, etc.

[0024] As a preferred embodiment of the emulsion of the present invention, the emulsion further comprises at least one of a moisturizer, a thickener, a preservative, and a pH regulator.

[0025] As a preferred embodiment of the emulsion of the present invention, the emulsion comprises the following components in mass percentage: 1-5% of the tea polyphenol composition of the present invention, 5-25% of a moisturizer, 0.2-5% of a thickener, 0.05-1% of a preservative, 0.1-0.3% of a pH regulator, and the balance deionized water.

[0026] As a preferred embodiment of the emulsion of the present invention, the moisturizing agent includes at least one of caprylic / capric triglyceride, glycerin, butylene glycol, and polydimethylsiloxane.

[0027] As a preferred embodiment of the emulsion of the present invention, the thickener includes at least one of cetearyl alcohol and xanthan gum.

[0028] As a preferred embodiment of the emulsion of the present invention, the preservative includes at least one of p-hydroxyacetophenone and 1,2-hexanediol.

[0029] As a preferred embodiment of the emulsion of the present invention, the pH regulator includes triethanolamine.

[0030] The present invention has found that when the components are further selected to be of the above types and the mass percentage of the components is within the above range, the obtained emulsion has better stability and better short-term and long-term skin brightening and firming effects.

[0031] In a fourth aspect of the present invention, the present invention provides a method for preparing the emulsion, comprising the following steps:

[0032] (1) caprylic / capric triglyceride, cetearyl alcohol, and polydimethylsiloxane are mixed, heated, stirred, and dissolved to obtain an oil phase;

[0033] (2) adding glycerol, butylene glycol, and xanthan gum to water, heating and stirring to dissolve, to obtain an aqueous phase;

[0034] (3) adding the oil phase to the water phase under stirring and emulsifying to obtain a uniformly emulsified crude emulsion;

[0035] (4) cooling the crude emulsion, adding α-glucan oligosaccharide and stirring evenly, then adding oxidized resveratrol and heating and stirring;

[0036] (5) After heating and stirring, add the freeze-dried mixture of tea polyphenols and antioxidants and stir evenly, then cool down and adjust the pH value with triethanolamine, and finally add pre-dissolved p-hydroxyacetophenone and 1,2-hexanediol aqueous solution and mix evenly to obtain an emulsion.

[0037] The present invention has found that when the preparation method of the present invention is used for preparation, specifically, by sequentially adding α-glucan oligosaccharide, oxidized resveratrol, and a freeze-dried mixture of tea polyphenols and antioxidants after cooling the obtained crude emulsion, the stability of the obtained emulsion can be better improved, and the short-term and long-term skin brightening and firming effects of the emulsion can be made better.

[0038] As a preferred embodiment of the preparation method of the present invention, in step (1), the temperature of heating and stirring is 75-80°C.

[0039] As a preferred embodiment of the preparation method of the present invention, in the step (2), the temperature of heating and stirring is 75-80°C.

[0040] As a preferred embodiment of the preparation method of the present invention, in step (3), the rotation speed of the stirring and emulsification is 1000-1500 rpm, and the stirring and emulsification time is 15-20 min.

[0041] As a preferred embodiment of the preparation method of the present invention, in step (4), the temperature after cooling is 40-45°C.

[0042] As a preferred embodiment of the preparation method of the present invention, in the step (4), the temperature of heating and stirring is 50±2°C.

[0043] As a preferred embodiment of the preparation method of the present invention, in step (5), the temperature after cooling is 40±2°C.

[0044] As a preferred embodiment of the preparation method of the present invention, in the step (5), the pH value is adjusted to 6.5-7.5.

[0045] As a preferred embodiment of the preparation method of the present invention, in the step (5), the freeze-drying temperature is -20°C to -40°C, the freeze-drying pressure is 10Pa to 100Pa, and the freeze-drying time is 1.5h to 3h.

[0046] Compared with the prior art, the present invention has the following beneficial effects:

[0047] The present invention can effectively improve the stability of the composition by selecting components in appropriate mass parts and cooperating with each other. The components also work together to better achieve short-term and long-term skin whitening, brightening and firming effects. DETAILED DESCRIPTION

[0048] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0049] Unless otherwise specified, the reagents, methods, and equipment used in the present invention are all conventional reagents, methods, and equipment in the art; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0050] Tea polyphenols 1: commercially available.

[0051] Tea polyphenol 2: Freeze-dry tea polyphenol 1 at -30±5°C and 50 Pa for 2 h to obtain tea polyphenol 2.

[0052] Oxyresveratrol: Commercially available.

[0053] α-Glucan oligosaccharide: Beijing Mairuida Technology Co., Ltd., 27707-45-5.

[0054] Examples 1-6 and Comparative Examples 1-4

[0055] The examples and comparative examples of the present invention provide a tea polyphenol composition, the components (parts by mass) of the tea polyphenol composition are shown in Table 1;

[0056] Table 1

[0057]

[0058]

[0059] The preparation method of the tea polyphenol composition provided in Example 1 is: mixing the components uniformly to obtain the tea polyphenol composition.

[0060] The preparation methods of the tea polyphenol compositions provided in Examples 2-6 and Comparative Examples 1-4 are consistent with that in Example 1.

[0061] Comparative Example 5

[0062] The comparative example of the present invention provides a tea polyphenol composition. The only difference between the tea polyphenol composition and Example 1 is that tea polysaccharides are used instead of tea polyphenols.

[0063] Comparative Example 6

[0064] The comparative example of the present invention provides a tea polyphenol composition. The only difference between the tea polyphenol composition and Example 1 is that resveratrol is used instead of oxidized resveratrol.

[0065] Comparative Example 7

[0066] The comparative example of the present invention provides a tea polyphenol composition. The only difference between the tea polyphenol composition and Example 1 is that glucan is used instead of α-glucan oligosaccharide.

[0067] Effect Example 1

[0068] The present invention investigates the free radical scavenging effects of the tea polyphenol compositions prepared in Examples 1-6 and Comparative Examples 1-7, specifically:

[0069] 1. ABTS+ free radical scavenging:

[0070] Reagents: ABTS reagent, potassium persulfate, ethanol, phosphate buffered saline (PBS, pH 7.4), etc.

[0071] Instruments: spectrophotometer, electronic balance, constant temperature water bath, pipette, volumetric flask, etc.

[0072] ABTS stock solution: Weigh 0.134 g of ABTS reagent, dissolve it in ethanol and dilute to 10 mL to prepare a 7 mmol / L ABTS stock solution. Protect from light.

[0073] Potassium persulfate solution: Weigh 0.038 g of potassium persulfate and dissolve it in deionized water to prepare 10 mL of a solution with a concentration of 2.45 mmol / L. Prepare it before use.

[0074] ABTS+ free radical working solution: Mix ABTS stock solution with potassium persulfate solution in a 1:1 volume ratio. Incubate in the dark at room temperature for 12-16 hours to obtain the ABTS+ free radical solution. Dilute with PBS before use to an absorbance of 0.70 ± 0.02 at 734 nm. To prepare the ABTS+ free radical working solution, take 1 mL of the mixed solution and dilute to 10 mL with PBS. Measure the absorbance. If the absorbance is not within the range of 0.70 ± 0.02, adjust the dilution factor until the absorbance reaches the desired value.

[0075] The samples to be tested were dissolved in ethanol to prepare different sample solutions.

[0076] Reaction process: Place 1.0 mL of ABTS + free radical working solution in a cuvette, add 0.1 mL of sample solution, and mix quickly. Simultaneously, set up a blank control group, which consists of 1.0 mL of ABTS + free radical working solution and 0.1 mL of the corresponding solvent, without adding sample.

[0077] Absorbance measurement: After the reaction system was reacted at room temperature in the dark for 8 minutes, the absorbance was measured at a wavelength of 734 nm using a spectrophotometer. Record the absorbance A1 of the sample group, the absorbance A0 of the blank control group, and the absorbance A of the control group (vitamin E). C Calculate the clearance rate: Calculate the clearance rate of the sample to ABTS+ free radicals according to the formula: Clearance rate (%) = (A 0- A1) / A0×100%;

[0078] The results are shown in Table 2;

[0079] Table 2 Antioxidant test results

[0080] Group <![CDATA[IC 50 (ABTS + )]]> Example 1 0.005％ Example 2 0.012％ Example 3 0.006％ Example 4 0.011％ Example 5 0.015％ Example 6 0.009％ Comparative Example 1 2.154％ Comparative Example 2 1.398％ Comparative Example 3 0.231％ Comparative Example 4 0.154％ Comparative Example 5 0.983％ Comparative Example 6 0.487％ Comparative Example 7 0.599％ control group 0.020％

[0081] It can be seen from Table 2 that when the technical solution provided by the present invention is adopted, the obtained tea polyphenol composition has an excellent effect of scavenging free radicals.

[0082] It can be seen from Examples 1-6 and Comparative Examples 1-2 that when oxidized resveratrol or α-glucan oligosaccharide is not added to the tea polyphenol composition, the free radical scavenging effect of the obtained tea polyphenol composition is significantly reduced; it can be seen from Examples 1-6 and Comparative Examples 3-4 that when the mass parts of the components in the tea polyphenol composition are not within the range given in the present invention, the free radical scavenging effect of the obtained tea polyphenol composition also shows a certain downward trend; it can be seen from Examples 1-6 and Comparative Examples 5-7 that when the components in the tea polyphenol composition are replaced with other similar ingredients, the obtained tea polyphenol composition cannot achieve the effect of the present invention.

[0083] Effect Example 2

[0084] The present invention investigates the stability of the tea polyphenol compositions prepared in Examples 1-6 and Comparative Examples 1-7. The specific tests are as follows:

[0085] (1) Heat resistance test: Place the sample in an electric constant temperature incubator and adjust the temperature to 40±1°C. Take it out and place it at room temperature on the 1st, 7th, and 45th day respectively. Observe whether there are any abnormal phenomena such as discoloration, stratification, precipitation, or taste change.

[0086] (2) Cold resistance test: Place the sample in a constant temperature refrigerator and adjust the temperature to -15±1°C. Take it out and place it at room temperature on the 1st, 7th, and 45th day respectively. Observe whether there are any abnormal phenomena such as discoloration, stratification, precipitation, or taste change.

[0087] The results are shown in Table 3;

[0088] Table 3 Stability test of different tea polyphenol compositions

[0089]

[0090]

[0091] As can be seen from Table 3, the tea polyphenol composition prepared by the technical solution of the present invention has excellent stability. As can be seen from Example 1 and Comparative Examples 1-2, when a certain component is missing, the obtained product becomes unstable and the yellow color deepens on the 7th day under high temperature conditions. As can be seen from Example 1 and Comparative Examples 5-7, when similar ingredients are used to replace the components of the present invention, the high-temperature stability and low-temperature stability of the obtained product show a significant downward trend.

[0092] Application Examples 1-8, Comparative Application Examples 1-7

[0093] The application examples of the present invention and the comparative application examples provide an emulsion, the components (mass percentage) of the emulsion are shown in Table 4; wherein the tea polyphenol compositions used in application examples 1-6 are respectively the tea polyphenol compositions prepared in embodiments 1-6, for example, the tea polyphenol compositions used in application example 1 are respectively the tea polyphenol compositions prepared in embodiment 1, and so on, the tea polyphenol compositions used in comparative application examples 1-7 are respectively the tea polyphenol compositions prepared in comparative examples 1-7; the tea polyphenol compositions used in application examples 7-8 are the tea polyphenol compositions prepared in embodiment 1;

[0094] In addition, a blank application example was set up, wherein the only difference between the blank application example and application example 1 was that no tea polyphenol composition was added;

[0095] Table 4 Composition of emulsion

[0096]

[0097] The preparation method of the emulsion provided in Application Example 1 comprises the following steps:

[0098] (1) Add caprylic / capric triglyceride, cetearyl alcohol, and polydimethylsiloxane to the oil phase pot, heat to 77±1°C, and stir evenly to completely dissolve them to obtain the oil phase;

[0099] (2) Add deionized water, glycerol, butylene glycol, and xanthan gum to the water phase pot, stir evenly, and heat to 77±1°C to fully dissolve the xanthan gum and disperse it evenly to obtain the water phase;

[0100] (3) Slowly add the oil phase to the water phase pot while stirring at 1200 rpm. After the addition is completed, continue stirring and emulsifying at 1200 rpm for 18 minutes to obtain a uniformly emulsified crude emulsion;

[0101] (4) Cooling the uniformly emulsified crude emulsion to 42 ± 1 °C, adding α-glucan oligosaccharide and stirring evenly, then adding oxidized resveratrol and stirring and heating to 50 °C;

[0102] (5) After heating to 50°C, add the mixture of tea polyphenols and antioxidants and stir evenly to fully disperse them in the emulsion. Then cool to 40°C and add triethanolamine to adjust the pH value of the emulsion to 6.5-7.5. Finally, add pre-dissolved p-hydroxyacetophenone and 1,2-hexanediol aqueous solution and stir evenly to obtain an emulsion.

[0103] The preparation methods of the emulsions provided in Application Examples 2-8, Comparative Application Examples 1-7 and Blank Application Examples are consistent with that in Application Example 1, and no relevant components are added if they are not present.

[0104] Comparative Application Example 8

[0105] The application example of the present invention and the comparative application example provide an emulsion. The difference between the emulsion and application example 1 is the preparation method. The preparation method of the emulsion provided in this comparative application example includes the following steps:

[0106] (1) Add caprylic / capric triglyceride, cetearyl alcohol, and polydimethylsiloxane to the oil phase pot, heat to 77±1°C, and stir evenly to completely dissolve them to obtain the oil phase;

[0107] (2) Add deionized water, glycerol, butylene glycol, and xanthan gum to the water phase pot, stir evenly, and heat to 77±1°C to fully dissolve the xanthan gum and disperse it evenly to obtain the water phase;

[0108] (3) Slowly add the oil phase to the water phase pot while stirring at 1200 rpm. After the addition is completed, continue stirring and emulsifying at 1200 rpm for 18 minutes to obtain a uniformly emulsified crude emulsion;

[0109] (4) Cooling the uniformly emulsified crude emulsion to 42±1°C, adding α-glucan oligosaccharide, oxidized resveratrol and tea polyphenols and stirring evenly, then cooling to 40°C and adding triethanolamine to adjust the pH value of the emulsion to 6.5-7.5; finally, adding pre-dissolved p-hydroxyacetophenone and 1,2-hexanediol aqueous solution and stirring evenly to obtain an emulsion.

[0110] Effect Example 3

[0111] The present invention investigates the stability of the emulsions prepared in Application Examples 1-8, Comparative Application Examples 1-8, and Blank Application Examples. The specific test is the same as the test of the stability of the tea polyphenol composition in Effect Example 2. The results are shown in Table 5.

[0112] Table 5 Stability test of different tea polyphenol compositions applied in emulsion

[0113]

[0114]

[0115]

[0116] As can be seen from Table 5, the emulsions prepared using the technical solution of the present invention have excellent stability. As can be seen from Application Examples 1-6 and Comparative Application Examples 1-2, when no oxidized resveratrol or α-glucan oligosaccharide is added to the tea polyphenol composition in the emulsion, the obtained emulsion becomes unstable and precipitates after 45 days at both high and low temperatures. As can be seen from Application Examples 1-6 and Comparative Application Examples 5-7, when the components in the tea polyphenol composition in the emulsion are replaced with other similar components, the stability of the obtained emulsion decreases significantly, becoming unstable and precipitating after 7 days at high temperature and also becoming unstable and precipitating after 45 days at low temperature. As can be seen from Application Examples 1-6 and Comparative Application Example 8, when the preparation method of the emulsion is not within the scope of the present invention, the stability of the obtained product also shows a certain downward trend.

[0117] Effect Example 4

[0118] The effect examples of the present invention explore the brightening and firming effects of the emulsions prepared in Application Examples 1-8, Comparative Application Examples 1-8 and Blank Application Examples on human skin, specifically including the following contents:

[0119] 1 test sample

[0120] 1.1. Sample Usage

[0121] The emulsions prepared in Application Examples 1-8, Comparative Application Examples 1-8 and Blank Application Examples were evenly applied to the face using 2-3 pumps each time, and gently massaged until absorbed.

[0122] 1.2. Research Sample Requirements

[0123] Before the trial, the client needs to provide a signed and stamped product safety statement to guarantee the safety of the test samples. This safety statement is used to ensure that the test samples will not pose a foreseeable risk to the health of the subjects when used under the conditions specified in the protocol.

[0124] 2. Subjects

[0125] 2.1. Signed informed consent and voluntary participation in this study: All subjects provided verbal and written informed consent in accordance with local regulations and procedures. This informed consent explained the nature, purpose, and potential risks of the study and emphasized the voluntary nature of participation. Subjects were allowed to withdraw from the study at any time for any reason. All subjects were given ample time to consider the study before signing. All informed consent forms must be signed before the study begins.

[0126] 2.2. Inclusion Criteria

[0127] 1) Chinese women;

[0128] 2) Age 35-60 years old (at the start of the trial);

[0129] 3) Sagging facial skin;

[0130] 4) Gloss DSC of the test area ≤ 7a.u;

[0131] 5) Patients without severe systemic diseases, immunodeficiency or autoimmune diseases, and the tested area has not received skin treatment, cosmetic treatment or other tests that may affect the results;

[0132] 6) The test site has not participated in other clinical trials currently or in the past month;

[0133] 7) No cosmetic surgery such as laser or chemical peels;

[0134] 8) Stop using skin care products of the same type as the test sample during the trial;

[0135] Exclusion criteria

[0136] 1) Patients who have used antihistamines in the past week or immunosuppressants or immunomodulatory biological agents and small molecule drugs in the past month;

[0137] 2) Use of any anti-inflammatory drugs in the test area in the past two months;

[0138] 3) Patients with clinically unresolved inflammatory skin diseases;

[0139] 4) Women who are breastfeeding or pregnant;

[0140] 5) Bilateral mastectomy and bilateral axillary lymph node resection;

[0141] 6) People with highly sensitive constitution;

[0142] 7) People with allergic constitution, allergic dermatitis, or a history of skin diseases;

[0143] 8) In addition, the trial leader judges that the population is not suitable as the subjects of this trial.

[0144] 2.4. Restrictions

[0145] 1) During the trial, subjects should maintain the same living and eating habits as before participating in the trial;

[0146] 2) During the trial, any treatment that may affect the test area is prohibited;

[0147] 3) During the test, it is prohibited to use other similar skin care products or other products that may affect the test on the test area;

[0148] 4) During the test, daily necessities such as shower gel, soap, shampoo, etc. should be the same as those used before the test.

[0149] 2.5. Target number of cases

[0150] A total of 108 qualified subjects were selected, and it was guaranteed that at least 85 people would be included in the final study, that is, at least 5 people in each group.

[0151] 2.6. Screening failure, subject dropout and replacement

[0152] Unenrolled subjects: subjects who signed the informed consent form but were not enrolled because they did not meet the inclusion / exclusion criteria. This needs to be supplemented.

[0153] If a subject leaves the study after randomization or group assignment, it is considered a dropout. If a subject does not complete the study for any reason, it is considered a dropout and no additional enrollment will be made.

[0154] Shedding guidelines:

[0155] - Subject dropout consent: Subjects can withdraw at any time for any reason (according to the Declaration of Helsinki);

[0156] - Adverse event / serious adverse event: any event that the investigator believes is detrimental to the subject in continuing the trial;

[0157] - Failure to follow the protocol;

[0158] - Participants lost to follow-up;

[0159] - Other reasons: other reasons why the subject withdrew.

[0160] >In other cases, the researcher believes that continued participation in the trial would be detrimental to the subject;

[0161] >This client is terminated.

[0162] The CRF form must be filled out until the candidate fails the screening or is dropped out.

[0163] All subjects whose trial ends prematurely must be recorded by the investigator or research team on the flow sheet, the last page "End of Study", the Adverse Events Sheet and / or the Concomitant Therapy Sheet.

[0164] 3. Test equipment

[0165] ① Skin elasticity tester Cutometer MPA580 (Courage & Khazaka, Germany);

[0166] ② Glossymeter GL200 (Courage+Khazaka, Germany).

[0167] 4. Test Environment

[0168] Environmental requirements: Temperature: 21.0°C±1.0°C; Humidity: 50%±10%.

[0169] 5. Testing process

[0170] Experimental design

[0171] A single-center open-label, 2-week and 4-week before-after comparison trial (an error of 1 day is acceptable).

[0172] 5.2. Experimental process

[0173] ① During the first visit, the subjects were given an explanation of the experiment and signed an informed consent form.

[0174] ② On the morning of the test, participants should not use any facial products (including skincare, makeup, and medications). After cleansing their faces with water, they should enter a constant temperature and humidity chamber and sit quietly for 30 minutes. After 30 minutes, participants were screened and then tested with the instrument. A total of 108 participants were screened for inclusion, with a goal of ensuring that at least 90 participants were included.

[0175] ③ After the test is completed, the subjects will be instructed on product usage. After listening to the instructions, the product will be distributed and the subjects will use the test sample at home for 2 and 4 consecutive weeks. After 2 and 4 weeks of continuous product use according to the test instructions, the subjects will return to the site for a follow-up visit and perform instrument testing according to step ②.

[0176] ④Confirm the usage of the product at the beginning and end of the test.

[0177] 5.3. The test samples can be used for 2 weeks or 4 weeks continuously.

[0178] 5.4. Observe the test site

[0179] face.

[0180] 5.5. Test schedule

[0181] The test schedule is shown in Table 6, where "O" indicates implementation and " / " indicates non-implementation;

[0182] Table 6 Test schedule

[0183]

[0184] 5.6. Observation test items

[0185] > Instrumental testing was performed on the subjects’ faces three times before use, 2 weeks after use, and 4 weeks after use.

[0186] Skin firmness: Use a Cutometer to test skin firmness three times and take the average value. The smaller the value, the better the skin firmness.

[0187] Result judgment: After using the product, the skin firmness parameter value of the test area decreased significantly, indicating that the test sample has the effect of improving skin firmness.

[0188] Skin glossiness test: Use a Glossymeter to test three times and take the average Gloss DSC value. The higher the parameter value, the better the skin gloss.

[0189] Result judgment: After using the product, the gloss DSC of the test area increased significantly, indicating that the test product has the effect of improving skin gloss.

[0190] The results are shown in Table 7;

[0191] Table 7 Test results of different tea polyphenol compositions applied to body firming and brightening lotions

[0192]

[0193]

[0194] As can be seen from Table 7, when the technical solution provided by the present invention is used, the obtained emulsion has excellent immediate and long-lasting skin brightening and firming effects. Specifically, the obtained emulsion has a firmness change rate of more than 103.65% and a brightness change rate of more than 82.97% after 2 weeks; and a firmness change rate of more than 149.31% and a brightness change rate of more than 119.77% after 4 weeks.

[0195] It can be seen from Application Examples 1-8 and Comparative Application Examples 1-2 that when the tea polyphenol composition in the prepared emulsion does not include oxidized resveratrol or α-glucan oligosaccharide, the corresponding effect cannot be achieved; it can be seen from Application Examples 1-8 and Comparative Application Examples 3-4 that when the mass parts of the components in the tea polyphenol composition in the prepared emulsion are not within the range given by the present invention, the brightening and firming abilities of the obtained emulsion are significantly reduced; it can be seen from Application Examples 1-8 and Comparative Application Examples 5-7 that when the components in the tea polyphenol composition in the prepared emulsion are replaced by other similar ingredients, the corresponding effect cannot be achieved; it can be seen from Application Examples 1-8 and Comparative Application Example 8 that when the emulsion does not adopt the preparation method provided by the present invention, the comprehensive effect of the obtained emulsion is also significantly reduced.

[0196] Finally, it should be noted that the above embodiments are intended to illustrate the technical solutions of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A tea polyphenol composition with brightening and firming effects, characterized in that: The tea polyphenol composition comprises the following components in parts by weight: 0.5-1 parts of tea polyphenols, 0.1-0.3 parts of oxidized resveratrol, and 1-3 parts of α-glucan oligosaccharides.

2. The tea polyphenol composition according to claim 1, characterized in that The tea polyphenol composition comprises the following components in parts by weight: 0.7-0.8 parts of tea polyphenols, 0.15-0.25 parts of oxidized resveratrol, and 1.5-2.5 parts of α-glucan oligosaccharides.

3. The tea polyphenol composition according to claim 1, characterized in that The tea polyphenol composition also includes an antioxidant, which includes at least one of vitamin C and vitamin E.

4. The tea polyphenol composition according to claim 1, characterized in that Calculated based on the mass of tea polyphenols, the mass percentage of the antioxidant is 0.1-0.6%.

5. Use of the tea polyphenol composition according to any one of claims 1 to 4 in the preparation of cosmetics.

6. An emulsion, characterized in that The emulsion comprises the tea polyphenol composition according to any one of claims 1 to 4.

7. The emulsion according to claim 6, characterized in that Based on the total mass of the emulsion, the mass percentage of the tea polyphenol composition is 1-5%.

8. The emulsion according to claim 6, characterized in that The emulsion further comprises at least one of a moisturizer, a thickener, a preservative, and a pH adjuster.

9. The emulsion according to claim 8, characterized in that The moisturizing agent includes at least one of caprylic / capric triglyceride, glycerin, butylene glycol, and polydimethylsiloxane; And / or, the thickener includes at least one of cetearyl alcohol and xanthan gum; and / or, the preservative comprises at least one of p-hydroxyacetophenone and 1,2-hexanediol; And / or, the pH adjuster comprises triethanolamine.

10. The method for preparing the emulsion according to claim 9, wherein The preparation method comprises the following steps: (1) caprylic / capric triglyceride, cetearyl alcohol, and polydimethylsiloxane are mixed, heated, stirred, and dissolved to obtain an oil phase; (2) adding glycerol, butylene glycol, and xanthan gum to water, heating and stirring to dissolve, to obtain an aqueous phase; (3) adding the oil phase to the water phase under stirring and emulsifying to obtain a uniformly emulsified crude emulsion; (4) cooling the crude emulsion, adding α-glucan oligosaccharide and stirring evenly, then adding oxidized resveratrol and heating and stirring; (5) After heating and stirring, add the freeze-dried mixture of tea polyphenols and antioxidants and stir evenly, then cool down and adjust the pH value with triethanolamine, and finally add pre-dissolved p-hydroxyacetophenone and 1,2-hexanediol aqueous solution and mix evenly to obtain an emulsion.

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