Gel with antibacterial and anti-inflammatory effects and preparation method thereof

Abstract

The invention discloses gel with antibacterial and anti-inflammatory effects and a preparation method thereof, and belongs to the technical field of gel for decontamination. The gel is prepared from the following components in parts by weight: 10 to 25 parts of an abelmoschus manihot flavone extract, 10 to 30 parts of an abelmoschus manihot polysaccharide extract, 1 to 3 parts of a gel matrix, 5 to 10 parts of a humectant, 5 to 10 parts of a surfactant and 0.2 to 1 part of a preservative. The flavone and the polysaccharide are separated from the abelmoschus manihot through an ultrasonic-assisted extraction technology, and a stable hydrogel system is formed by combining the flavone and the polysaccharide with a gel matrix. By optimizing the extraction process of the active ingredients of the abelmoschus manihot and combining a gel delivery system, the synergistic antibacterial and anti-inflammatory effects of flavone and polysaccharide are achieved, and the product is stable in performance, high in safety and suitable for clinical and family decontamination scenes.

Description

Technical Field

[0001] The present invention relates to the technical field of gel for disinfection, in particular to a gel with antibacterial and anti-inflammatory effects and a preparation method thereof. Background Art

[0002] Disinfection products play an important role in healthcare, public health, and daily life. However, existing products often rely on chemically synthesized ingredients (such as chlorhexidine and benzalkonium chloride), which can lead to drug resistance, skin irritation, and microbial imbalances with long-term use. Natural plant extracts have garnered attention in recent years for their broad-spectrum antimicrobial properties and safety, but they suffer from issues such as low active ingredient extraction yields, poor stability, and unclear synergistic effects.

[0003] Abelmoschus manihot is a plant of the Malvaceae family, and its petals, stems and leaves are rich in flavonoids and polysaccharide compounds. In the prior art, flavonoids or polysaccharides are mostly used alone, and the combination of the two is not fully utilized, and there is a lack of stable formulations suitable for disinfection scenarios. In addition, the yield of flavonoids in traditional extraction processes is limited by insufficient solvent polarity matching, and polysaccharides are easily degraded, resulting in loss of active ingredients. Therefore, it is of great application value to develop an efficient extraction process and combine it with a gel carrier to prepare a disinfection product that has both rapid sterilization, long-lasting anti-inflammatory properties, and is mild and non-irritating. Summary of the Invention

[0004] The present invention aims to provide a gel with antibacterial and anti-inflammatory effects and a method for preparing the same, in order to address the above-mentioned problems in the prior art. The gel prepared by the present invention has highly effective antibacterial and anti-inflammatory properties, is mild and non-irritating, and is suitable for both clinical and household disinfection scenarios.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] Technical solution 1: A gel with antibacterial and anti-inflammatory effects, the components of which include sunflower flavonoids extract and sunflower polysaccharide extract.

[0007] Furthermore, the invention comprises the following components in parts by weight: 10-25 parts of flavonoids extract of Helianthus annuus, 10-30 parts of polysaccharide extract of Helianthus annuus, 1-3 parts of gel matrix, 5-10 parts of moisturizing agent, 5-10 parts of surfactant and 0.2-1 part of preservative.

[0008] Furthermore, the gel matrix is one or more of carbomer 934, carbomer 940, carbomer 980, sodium carboxymethyl cellulose, sodium alginate, gelatin, and aluminum distearate; the moisturizer is one or more of glycerin and propylene glycol; the surfactant is one or more of polysorbate 40, polysorbate 60, polysorbate 80, and sodium lauryl sulfate; and the preservative is one or more of sodium benzoate, potassium sorbate, and chlorobutanol.

[0009] Furthermore, the gel matrix is carbomer 940, the moisturizer is glycerin, the surfactant is polysorbate 40, and the preservative is sodium benzoate.

[0010] Furthermore, the preparation method of the sunflower flavonoids extract includes: ultrasonically extracting the crushed sunflower petals with a 70% volume concentration ethanol solution at 50-60°C for 2-3 times, each time for 45 minutes, combining the extracts and concentrating under reduced pressure, freeze-drying to obtain a crude flavonoid extract, and purifying to obtain the sunflower flavonoids extract.

[0011] Furthermore, the preparation method of the Helianthus annuus polysaccharide extract includes: reflux extraction of crushed Helianthus annuus stems and leaves with deionized water at 85-95°C for 2-3 hours, centrifugation to obtain the supernatant, eluting the protein, precipitating the polysaccharide with anhydrous ethanol, and freeze-drying to obtain the Helianthus annuus polysaccharide extract.

[0012] Furthermore, the pH value of the gel is 5.8.

[0013] Technical Solution 2: A method for preparing the gel, comprising the following steps:

[0014] The gel matrix is dispersed in deionized water, a moisturizer is added after swelling, and the mixture is stirred evenly to obtain a matrix solution; a flavonoid extract of sunflower seedlings, a polysaccharide extract of sunflower seedlings, a surfactant, and a preservative are dissolved in deionized water, and ultrasonic dispersion is performed to obtain an active ingredient solution; the obtained active ingredient solution is added to the matrix solution, homogenized, and the pH value is adjusted to obtain the gel.

[0015] Furthermore, the homogenization speed is 4000 rpm, and the homogenization time is 25 minutes.

[0016] Technical solution three: Application of the gel in the preparation of disinfectant products.

[0017] The present invention discloses the following technical effects:

[0018] The present invention provides an antibacterial and anti-inflammatory gel prepared based on flavonoids and polysaccharides from golden sunflower. The extraction process of the active ingredients is optimized by ultrasound-assisted extraction technology, which significantly improves the yield and purity of flavonoids and polysaccharides. The gel formula adopts excipients such as carbomer 940, glycerol, polysorbate 40 and sodium benzoate, combined with a homogenization process (4000rpm, 25 minutes) and pH adjustment to form a stable hydrogel system, which still maintains colloidal stability after 2 months of high temperature and light testing at 55°C. Antibacterial experiments show that the antibacterial rates of the gel in Example 1 against Escherichia coli and Staphylococcus aureus are 96.2% and 95.1%, respectively, which are significantly better than the control group with a single ingredient or unoptimized process (such as the antibacterial rate of comparative example 4 is only 67.7%), verifying the synergistic effect of flavonoids and polysaccharides. The gel has the characteristics of high efficiency antibacterial, anti-inflammatory, mild and non-irritating, and is suitable for clinical and home disinfection scenarios. Given that both sunflower flavonoids and sunflower polysaccharides have anti-inflammatory effects, the gel material containing sunflower flavonoids and sunflower polysaccharides prepared by the present invention can also be used for the research and development of anti-inflammatory products. Therefore, the gel prepared by the present invention has important application value in the field of antibacterial and anti-inflammatory. DETAILED DESCRIPTION

[0019] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0020] It should be understood that the terms described herein are intended only to describe particular embodiments and are not intended to limit the present invention. In addition, for numerical ranges herein, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. The intermediate value within any stated value or stated range, and each smaller range between any other stated value or intermediate value within the stated range, is also encompassed within the present invention. The upper and lower limits of these smaller ranges may be independently included or excluded within the scope.

[0021] Unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein may also be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of any conflict with any incorporated document, the contents of this specification shall prevail.

[0022] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from the description of the invention. The description and examples are intended to be exemplary only.

[0023] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0024] Example 1

[0025] 1. Preparation of Heliotropium flavonoids extract

[0026] (1) Take 1 kg of dried golden sunflower petals, crush them to 60 mesh, and add 15 L of 70% ethanol solution;

[0027] (2) Ultrasonic extraction (power 300 W, frequency 40 kHz) at 55°C twice, each time for 45 minutes;

[0028] (3) combining the extracts, concentrating under reduced pressure to obtain an extract, and freeze-drying to obtain a crude flavonoid;

[0029] (4) The crude flavonoids were dissolved in deionized water, chromatographed on an AB-8 macroporous resin column, eluted with 60% ethanol by volume, and the eluate was collected, concentrated, and freeze-dried to obtain a flavonoid extract.

[0030] 2. Preparation of Heliotropium polysaccharide extract

[0031] (1) Take 2 kg of stems and leaves of Heliotropium chrysantha, crush them and add 40 L of deionized water;

[0032] (2) Reflux extraction at 90°C for 2.5 hours, and centrifuge to remove the residue;

[0033] (3) The supernatant was deproteinized three times by the Sevage method (chloroform: n-butanol = 4:1);

[0034] (4) Add 3 times the volume of anhydrous ethanol to precipitate the polysaccharide, collect the precipitate by centrifugation, and freeze-dry to obtain the polysaccharide extract.

[0035] 3. Preparation of Decontamination Gel

[0036] (1) Weigh 9403 g of carbomer, disperse it in 30 mL of deionized water, and allow it to swell for 12 hours;

[0037] (2) Add 10 g of glycerol and stir to obtain a transparent matrix;

[0038] (3) 15 g of the Helianthus annuus flavonoids extract prepared in step 1, 25 g of the Helianthus annuus polysaccharide extract prepared in step 2, 406 g of polysorbate, and 0.4 g of sodium benzoate were dissolved in 40.6 mL of deionized water and ultrasonically dispersed to obtain a solution;

[0039] (4) Slowly add the solution from step (3) to the transparent matrix from step (2) and homogenize at 4000 rpm for 25 minutes;

[0040] (5) Add triethanolamine dropwise to adjust the pH to 5.8, and sterilize after filling to obtain the finished gel for later use.

[0041] Example 2

[0042] The difference from Example 1 is that the preparation of the disinfection gel in step 3 is: 1.5 g of carbomer 940, 10 g of glycerin, 3 g of Helianthus annuus flavonoid extract, 10 g of Helianthus annuus polysaccharide extract, 3 g of polysorbate 80, and 0.3 g of sodium benzoate. The remaining steps are the same as in Example 1.

[0043] Comparative Example 1

[0044] The difference from Example 1 is that the preparation of the flavonoid extract of Helianthus annuus in this comparative example is as follows:

[0045] (1) Take 1 kg of dried golden sunflower petals, crush them to 60 mesh, and add 15 L of 70% ethanol solution;

[0046] (2) Ultrasonic extraction (power 300 W, frequency 40 kHz) at 65°C for 45 min.

[0047] (3) concentrating the extract under reduced pressure to obtain an extract, and freeze-drying to obtain a crude flavonoid;

[0048] (4) The crude flavonoids were dissolved in deionized water, chromatographed on an AB-8 macroporous resin column, eluted with 60% ethanol by volume, and the eluate was collected, concentrated, and freeze-dried to obtain a flavonoid extract.

[0049] The remaining steps are the same as in Example 1.

[0050] Comparative Example 2

[0051] The difference from Example 1 is that the polysaccharide extract of Heliotrope chrysantha in this comparative example is prepared as follows:

[0052] (1) Take 2 kg of stems and leaves of Heliotropium chrysantha, crush them and add 40 L of deionized water;

[0053] (2) Reflux extraction at 100°C for 3.5 hours, and centrifuge to remove the residue;

[0054] (3) The supernatant was deproteinized three times by the Sevage method (chloroform: n-butanol = 4:1);

[0055] (4) Add 3 times the volume of anhydrous ethanol to precipitate the polysaccharide, collect the precipitate by centrifugation, and freeze-dry to obtain a polysaccharide extract (purity 88.3%).

[0056] The remaining steps are the same as in Example 1.

[0057] Comparative Example 3

[0058] The difference from Example 1 is that the preparation of the decontamination gel in this comparative example is as follows:

[0059] (1) Weigh 9404 g of carbomer, disperse it in 30 mL of deionized water, and allow it to swell for 12 hours;

[0060] (2) Add 13 g of glycerol and stir to obtain a transparent matrix;

[0061] (3) 35 g of the Helianthus annuus flavonoids extract prepared in step 1 above, 9 g of the Helianthus annuus polysaccharide extract prepared in step 2 above, 404 g of polysorbate, and 1.4 g of sodium benzoate were dissolved in 33.6 mL of deionized water and ultrasonically dispersed to obtain a solution;

[0062] (4) Slowly add the solution from step (3) to the transparent matrix from step (2) and homogenize at 4000 rpm for 25 minutes;

[0063] (5) Add triethanolamine dropwise to adjust the pH to 5.8, and sterilize after filling to obtain the finished gel for later use.

[0064] The remaining steps are the same as in Example 1.

[0065] Comparative Example 4

[0066] The difference from Example 1 is that in the preparation of the disinfectant gel in this comparative example, 40 g of Helianthus annuus flavonoid extract was used, and no Helianthus annuus polysaccharide extract was added. The remaining steps were the same as in Example 1.

[0067] Comparative Example 5

[0068] The difference from Example 1 is that in the preparation of the disinfectant gel in this comparative example, 40 g of Helianthus annuus polysaccharide extract was used, and no Helianthus annuus flavonoid extract was added. The remaining steps were the same as in Example 1.

[0069] Test Example 1

[0070] The finished gel products prepared in Example 1, Example 2 and Comparative Examples 1-5 were stored at 55° C. for 2 months and exposed to light for 2 months. The colloids of all the finished gel products remained stable and did not change color, indicating that the gel prepared by the preparation method of the present invention has stable performance.

[0071] Test Example 2

[0072] The purity of the flavonoid extract and the polysaccharide extract in Example 1, Comparative Example 1 and Comparative Example 2 was determined by high performance liquid chromatography. The determination process is as follows:

[0073] Chromatographic conditions: The chromatographic column was ZORBAX Eclipse XDB-C18 (Agilent; 150 mm length × 4.6 mm inner diameter; filled particle diameter was 5 μm); the mobile phase was acetonitrile-water (the volume ratio of acetonitrile and water was 77:23); the flow rate was 0.6 mL / min; the injection volume was 10 μL; the column temperature was 20°C; and the detection wavelength was 254 nm.

[0074] Standard curve: Take 5 mg of flavonoid extract or polysaccharide extract standard and dissolve it in 5 mL of methanol to prepare standard solutions with final concentrations of 500, 250, 125, 62.5, 31.25, and 0 μg / mL. The concentrations were injected in sequence from low to high, with an injection volume of 10 μL, and each concentration was repeated 3 times. The analysis was performed according to the above-mentioned chromatographic conditions. With the mass concentration of the flavonoid extract or polysaccharide extract as the vertical coordinate Y and the peak area of the flavonoid extract or polysaccharide extract as the horizontal coordinate X, a linear regression was performed to obtain the regression equation. In combination with the above-mentioned chromatographic conditions and standard curve, the purity of the purified flavonoid extract or polysaccharide extract in Example 1, Comparative Example 1 and Comparative Example 2 was determined respectively, and recorded in Table 1:

[0075] Table 1

[0076] Example 1 Comparative Example 1 Comparative Example 2 Purity of flavonoid extract 81.5％ 66.4％ 81.5％ Purity of polysaccharide extract 82.6％ 82.6％ 73.5％

[0077] As can be seen from Table 1, the extraction method of the present invention can obtain high-purity Helianthus annuus flavonoids and Helianthus annuus polysaccharides.

[0078] Test Example 3

[0079] The antibacterial performance of the gels prepared in Example 1, Example 2, and Comparative Examples 1-5 was tested. The performance testing process is as follows:

[0080] 0.1 g of the gel prepared in Example 1, Example 2 and Comparative Examples 1-5 were respectively mixed with 10 mL of 1×10 7 The mixture of Escherichia coli liquid and Staphylococcus aureus liquid was placed in a test tube and incubated on a constant temperature shaker at 37°C and 120 r / min for 4 h. This was used as the experimental group, and the one without antibacterial gel was used as the blank group. The bacterial liquid of the experimental group and the blank group was diluted to 1×10 5 CFU / mL, 100 μL of bacterial solution was spread on the surface of the agar plate, and placed in a shaking incubator at 37°C for 24 hours. The antibacterial rate was calculated by the plate colony counting method. The antibacterial rate calculation formula is as follows:

[0081] =[(Na-Nb) / Na]×100%, where Na is the number of colonies in the blank group and Nb is the number of colonies in the experimental group. The calculation results are recorded in Table 2.

[0082] Table 2

[0083]

[0084] As can be seen from Table 2, the gel material prepared by adding the flavonoids and polysaccharides extracted from Helianthus annuus L. of the present invention has excellent antibacterial properties.

[0085] The embodiments described above are merely descriptions of preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by persons skilled in the art should fall within the scope of protection defined by the claims of the present invention.

Claims

1. A gel with antibacterial and anti-inflammatory effects, characterized in that: The components include sunflower flavonoids extract and sunflower polysaccharide extract.

2. The gel according to claim 1, characterized in that The invention comprises the following components by weight: 10-25 parts of flavonoids extract of golden sunflower, 10-30 parts of polysaccharide extract of golden sunflower, 1-3 parts of gel matrix, 5-10 parts of moisturizing agent, 5-10 parts of surfactant and 0.2-1 part of preservative.

3. The gel according to claim 1, characterized in that The gel matrix is one or more of carbomer 934, carbomer 940, carbomer 980, sodium carboxymethyl cellulose, sodium alginate, gelatin, and aluminum distearate; the moisturizer is one or more of glycerin and propylene glycol; the surfactant is one or more of polysorbate 40, polysorbate 60, polysorbate 80, and sodium lauryl sulfate; and the preservative is one or more of sodium benzoate, potassium sorbate, and chlorobutanol.

4. The gel according to claim 3, characterized in that The gel matrix is carbomer 940, the moisturizer is glycerin, the surfactant is polysorbate 40, and the preservative is sodium benzoate.

5. The gel according to claim 1, characterized in that The preparation method of the flavonoid extract of golden sunflower comprises: subjecting crushed golden sunflower petals to ultrasonic extraction with a 70% volume concentration ethanol solution at 50-60° C. for 2-3 times, each time for 45 minutes, combining the extracts and concentrating under reduced pressure, freeze-drying to obtain a crude flavonoid extract, and purifying to obtain the golden sunflower flavonoid extract.

6. The gel according to claim 1, characterized in that The preparation method of the Helianthus annuus polysaccharide extract comprises the following steps: reflux extraction of crushed Helianthus annuus stems and leaves with deionized water at 85-95° C. for 2-3 hours, centrifugation to obtain the supernatant, eluting the protein, precipitating the polysaccharide with anhydrous ethanol, and freeze-drying to obtain the Helianthus annuus polysaccharide extract.

7. The gel according to claim 1, characterized in that The pH value of the gel was 5.

8.

8. A method for preparing the gel according to any one of claims 1 to 7, characterized in that: The following steps are involved: The gel matrix is dispersed in deionized water, a moisturizer is added after swelling, and the mixture is stirred evenly to obtain a matrix solution; a flavonoid extract of sunflower seedlings, a polysaccharide extract of sunflower seedlings, a surfactant, and a preservative are dissolved in deionized water, and ultrasonic dispersion is performed to obtain an active ingredient solution; the obtained active ingredient solution is added to the matrix solution, homogenized, and the pH value is adjusted to obtain the gel.

9. The preparation method according to claim 8, characterized in that The homogenization speed is 4000 rpm, and the homogenization time is 25 minutes.

10. Use of the gel according to any one of claims 1 to 7 in the preparation of disinfectant products.

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