Shampoo containing plant exosome and melanin and preparation method thereof

Abstract

The invention provides shampoo containing plant exosomes and melanin and a preparation method of the shampoo. The shampoo can effectively delay white hair, nourish scalp and improve hair quality by utilizing the characteristics of oxidation resistance, inflammation resistance, wrapping, delivery and the like of the plant exosome. The preparation method is simple and feasible and is suitable for large-scale production.

Description

Technical Field

[0001] The present invention relates to a shampoo, in particular to a shampoo containing plant exosomes and melanin and a preparation method thereof, particularly to the technical field of whitening-delaying shampoo. Background Art

[0002] The accelerated pace of life and increasing stress in modern society are causing gray hair to strike younger people, seriously impacting their appearance and mental health. Existing anti-gray hair products lack effective absorption by the scalp, and long-term use can damage the scalp and hair. Therefore, developing a natural, safe, and effective anti-gray hair shampoo is crucial.

[0003] The current common shampoo formula contains the following ingredients:

[0004] Surfactants are the main ingredients of shampoo. Anionic surfactants (lauryl sulfate, lauryl polyether sulfate, etc.) are used to clean grease and dirt on the hair and scalp.

[0005] Conditioners (cationic guar gum, emulsified silicone oil, etc.), thickeners, preservatives, fragrances, etc. are auxiliary ingredients of shampoo, which are used to improve the user experience and hair care effect of shampoo.

[0006] The formula of some shampoo products can be adjusted based on user feedback to achieve personalized care and meet consumers' personalized needs for shampoo products.

[0007] Existing technical solution CN201210214950.5 Eclipta prostrata black hair nourishing shampoo, black hair nourishing hair conditioner and black hair nourishing liquid: A Eclipta prostrata black hair nourishing shampoo, including deionized water, sodium salt of fatty alcohol polyoxyethylene ether sulfate, coconut oil diethanolamide, zwitterionic surfactant, silk protein, marine polysaccharide moisturizer, fresh Eclipta prostrata extract, essence and preservative.

[0008] Exosomes are nanoscale vesicles secreted by cells and rich in a variety of bioactive substances, including proteins, lipids, and RNA. In recent years, plant exosomes have garnered widespread attention due to their excellent biocompatibility and diverse bioactive functions. They possess antioxidant, anti-inflammatory, and damage-repairing properties, effectively promoting scalp health and improving hair quality.

[0009] Melanin, a biological pigment produced by melanocytes, is primarily responsible for the color of skin and hair. Melanin synthesis is influenced by a variety of factors, including genetics, environment, and age. With aging, melanocyte function gradually declines, leading to graying of hair.

[0010] Exosomes can encapsulate and protect melanin from damage by the external environment and deliver it to target cells through specific mechanisms. Studies have shown that exosomes can significantly promote the proliferation of melanocytes and melanin synthesis.

[0011] Exosomes exert their effects through the following pathways:

[0012] 1. Promote melanocyte proliferation: Exosomes contain a variety of growth factors and signaling molecules that stimulate melanocyte division and proliferation, thereby increasing melanin production. 2. Antioxidant and anti-inflammatory effects: Exosomes possess antioxidant and anti-inflammatory properties, protecting melanocytes from oxidative stress and inflammatory damage, maintaining their normal function. 3. Encapsulation and delivery: Exosomes can encapsulate and deliver melanin, protecting it from damage by the external environment and effectively delivering it to target cells.

[0013] The active ingredients of existing anti-gray hair products cannot be effectively absorbed by the scalp, and long-term use may cause damage to the scalp and hair. Summary of the Invention

[0014] The present invention primarily addresses the deficiencies of the prior art by providing a shampoo containing low-proportion plant exosomes and melanin as active ingredients. The shampoo provided by the present invention, by adding Polygonum multiflorum exosomes or Sapindus mukorossi exosomes and melanin, can effectively delay hair graying and promote melanin production and absorption. Furthermore, the shampoo is mild and non-irritating, with rich lather, leaving hair feeling refreshed and flowing, and the scalp clean and comfortable. This shampoo, containing plant exosomes and melanin, and its preparation method, combines hair cleansing and hair care.

[0015] The above technical problems of the present invention are mainly solved by the following technical solutions:

[0016] A shampoo containing plant exosomes and melanin and a preparation method thereof, comprising the following components by weight:

[0017] Surfactant: 10-20%; plant exosomes: 5-25%; melanin: 0.01-1%; moisturizer: 5-10%; antioxidant: 1-3%; flavor: 0.1-0.5%; physiological saline: balance.

[0018] Preferably, the plant exosomes are Polygonum multiflorum and Sapindus mukorossi.

[0019] A shampoo containing plant exosomes and melanin and a preparation method thereof are carried out according to the following steps:

[0020] (1) Preparation of plant exosomes:

[0021] Collect fresh Polygonum multiflorum and Sapindus mukorossi, clean them and chop them;

[0022] The chopped plant material was added to an appropriate amount of physiological saline and homogenized;

[0023] The homogenate was centrifuged and the supernatant was taken;

[0024] The supernatant is further separated by ultrafiltration or dialysis to obtain plant exosomes;

[0025] (2) Plant exosomes coated with melanin:

[0026] Mix exosomes and melanin in appropriate proportions;

[0027] By repeatedly freezing and thawing the exosomes three times, melanin was introduced;

[0028] (3) Preparation of shampoo:

[0029] Add surfactant, moisturizer, antioxidant and fragrance into normal saline in the above weight proportions in sequence and stir evenly;

[0030] Add the plant exosomes encapsulating melanin into the above mixture and continue stirring evenly;

[0031] The mixed solution is filtered and sterilized to obtain a shampoo containing plant exosomes.

[0032] Preferably, in step (1), the homogenate is centrifuged at 4°C, 10,000g, for 30 min, and the supernatant is collected.

[0033] Preferably, in step (2), the exosomes and melanin are mixed in a ratio of 1:100.

[0034] Preferably, in step (iii), the mixed solution is sterilized by filtering through a 0.22 μM filter membrane.

[0035] It aims to utilize the bioactive substances in plant exosomes that encapsulate melanin to nourish the scalp, improve hair quality and turn gray hair black.

[0036] The shampoo containing plant exosomes provided by the present invention utilizes the antioxidant, anti-inflammatory, and damage-repairing properties of plant exosomes to effectively nourish the scalp, improve hair quality, and reduce dandruff and hair loss. Furthermore, the preparation method of the present invention is simple and easy to implement, making it suitable for large-scale production.

[0037] Compared with traditional methods, this method has natural biological properties and can improve the delivery efficiency and biological absorption rate of melanin; secondly, the low immunogenicity and high stability of exosomes make them safer and more tolerable. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1This is a transmission electron microscope observation of the plant exosomes obtained in Example 1 of the present invention;

[0039] Figure 2 This is a graph showing the results of the particle size-concentration distribution analysis in Example 1 of the present invention;

[0040] Figure 3 This is a transmission electron microscope observation of the product in Example 1 of the present invention;

[0041] Figure 4 This is a comparison chart of the whitening function test of this product on volunteers in Example 1 of the present invention. DETAILED DESCRIPTION

[0042] The technical solution of the present invention is further specifically described below through examples.

[0043] Example 1: A shampoo containing plant exosomes and melanin, the components of which are as follows by weight:

[0044] The surfactant is sodium lauryl sulfate: 15%;

[0045] Plant exosomes are soapberry exosomes: 10%

[0046] Melanin: 0.05%

[0047] Moisturizing agent is glycerin: 8%

[0048] Antioxidant as Vitamin C: 2%

[0049] Rose essential oil: 0.3%

[0050] Normal saline: balance

[0051] The preparation method is as follows:

[0052] 1. Collect fresh soapberries, clean them, remove the core and chop them into small pieces, then add an appropriate amount of physiological saline for homogenization;

[0053] 2. The homogenate was centrifuged, and the supernatant was further separated by ultrafiltration to obtain soapberry exosomes;

[0054] The obtained plant exosomes were observed by transmission electron microscopy (e.g. Figure 1 ). Electron microscopy results showed that small vesicles also had a typical exosome saucer-like structure, with a bright bright circle on the edge of the vesicle and a darker interior, showing obvious membrane structural characteristics.

[0055] To further identify and characterize the extracted exosomes, the obtained plant exosomes were subjected to nanoparticle tracking analysis (e.g. Figure 2The results of particle size-concentration distribution analysis showed that the exosome particle size was about 100 nm, which was consistent with the results shown by electron microscopy.

[0056] 3. Mix the soapberry exosomes and melanin in proportion, and freeze and thaw the exosomes three times repeatedly to allow the exosomes to encapsulate the melanin;

[0057] 4. Add sodium lauryl sulfate, glycerin, vitamin C and rose essential oil to the normal saline solution in sequence and stir evenly;

[0058] 5. Add the prepared soapberry exosomes to the above mixture and continue stirring evenly;

[0059] 6. The mixed solution is filtered and sterilized to obtain a shampoo containing soapberry exosomes and melanin.

[0060] This product was observed under a transmission electron microscope (e.g. Figure 3 ), small vesicles were observed under electron microscopy, and the results showed that the vesicles also had a typical exosome saucer-like structure, indicating that the exosomes encapsulating melanin can be stably preserved in this product.

[0061] The whitening function of this product was tested on volunteers (such as Figure 4 ), experimental results show that using this product twice a week for 3 consecutive months can effectively turn white hair into black hair.

[0062] Example 2:

[0063] A shampoo containing plant exosomes and melanin, the components of which are as follows by weight:

[0064] The surfactant is cocamidopropyl betaine: 12%;

[0065] Plant exosomes include Polygonum multiflorum exosomes: 8%;

[0066] Melanin: 0.04%

[0067] Moisturizer is propylene glycol: 6%;

[0068] Antioxidant is vitamin E: 1%;

[0069] The fragrance is lemon essential oil: 0.2%;

[0070] Normal saline: balance.

[0071] The preparation method is similar to that of Example 1, except that the soapberry is replaced by Polygonum multiflorum.

Claims

1. A shampoo containing plant exosomes and melanin and a preparation method thereof, characterized in that By weight, it comprises the following components: Surfactant: 10-20%; plant exosomes: 5-25%; melanin: 0.01-1%; moisturizer: 5-10%; antioxidant: 1-3%; flavor: 0.1-0.5%; physiological saline: balance.

2. The shampoo containing plant exosomes and melanin and the preparation method thereof according to claim 1, characterized in that: The plant exosomes are Polygonum multiflorum and Sapindus mukorossi.

3. A shampoo containing plant exosomes and melanin and a preparation method thereof, wherein the preparation method uses the shampoo according to any one of claims 1 to 2, characterized in that: (1) Preparation of plant exosomes: Collect fresh Polygonum multiflorum and Sapindus mukorossi, clean them and chop them; Add the chopped plant material into an appropriate amount of physiological saline and homogenize it; The homogenate was centrifuged and the supernatant was taken; The supernatant is further separated by ultrafiltration or dialysis to obtain plant exosomes; (2) Plant exosomes coated with melanin: Mix exosomes and melanin in appropriate proportions; By repeatedly freezing and thawing the exosomes three times, melanin was introduced; (3) Preparation of shampoo: Add surfactant, moisturizer, antioxidant and fragrance into normal saline in the above weight proportions in sequence and stir evenly; Add the plant exosomes encapsulating melanin into the above mixture and continue stirring evenly; The mixed solution is filtered and sterilized to obtain a shampoo containing plant exosomes.

4. The method for preparing a product containing plant exosomes and melanin according to claim 3, wherein: In the step (1), the homogenate is centrifuged at 4° C., 10,000 g, for 30 min, and the supernatant is collected.

5. The method for preparing a product containing plant exosomes and melanin according to claim 3, wherein: In the step (2), the exosomes and melanin are mixed in a ratio of 1:

100.

6. The method for preparing a product containing plant exosomes and melanin according to claim 3, wherein: In the step (3), the mixed solution is sterilized by filtration using a 0.22 μM filter membrane.

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