Polymerase chain reaction device and control method thereof

Abstract

The present invention relates to the technical field of PCR instruments, and discloses a polymerase chain reaction device and a control method thereof, the polymerase chain reaction device comprises: a frame provided with a first driving member and a transverse guide rail; water in the constant-temperature water tanks is kept at different water temperatures; the material rack is provided with a plurality of material placing areas, and the plurality of material placing areas can be used for placing a plurality of reaction materials; the material frame moving module is movably arranged on the rack, and the first driving piece can drive the material frame moving module to slide along the transverse guide rail; the material frame moving module comprises a longitudinal guide rail, a second driving part and a grabbing manipulator, the longitudinal guide rail is perpendicular to the transverse guide rail, and the second driving part can drive the grabbing manipulator to slide up and down along the longitudinal guide rail; and the material frame is grabbed by the grabbing manipulator, so that the plurality of reaction materials on the material frame are turned over among the plurality of constant-temperature water tanks with different water temperatures. The polymerase chain reaction device has the advantages that the preparation efficiency of a standard product can be effectively improved, and the automation degree is high.

Description

Technical Field

[0001] The present invention relates to the technical field of PCR instruments, and in particular to a polymerase chain reaction device and a control method thereof. Background Art

[0002] A DNA ladder is a standard reference for nucleic acid fragment sizing in molecular biology experiments. Composed of a series of DNA fragments of known length, it is widely used in genetic analysis, electrophoresis quality control, and apoptosis detection. Its core function is to provide precise molecular weight comparisons based on electrophoretic mobility differences, ensuring the accuracy of experimental results.

[0003] DNA ladders typically contain DNA fragments ranging from 50 bp to 10,000 bp (base pairs), with different products covering specific gradients (e.g., 100 bp or 1 kb intervals). These fragments are prepared by enzyme digestion, PCR (polymerase chain reaction) amplification, or chemical synthesis, and their lengths are rigorously calibrated. During electrophoresis, the DNA to be tested migrates simultaneously with the ladder, forming separate bands due to differences in fragment length. By comparing the positions of these bands with those on the ladder, the molecular weight of the unknown DNA can be estimated.

[0004] Because nucleic acid fragment standards are usually prepared by PCR amplification, the single reaction volume of a conventional PCR instrument is generally limited to 20-50ul, and the heating and cooling speeds of the metal well plate are slow, so the output of standards produced by amplification is very limited. Summary of the Invention

[0005] In view of the above-mentioned deficiencies in the prior art, the technical problem to be solved by the present invention is to propose a polymerase chain reaction device and a control method thereof that can effectively improve the efficiency of standard preparation and has a high degree of automation.

[0006] The technical solution adopted by the present invention to solve the technical problem is to provide a polymerase chain reaction device, comprising:

[0007] a frame, wherein the frame is provided with a first driving member and a transverse guide rail;

[0008] A plurality of constant temperature water tanks are arranged in sequence along the length direction of the transverse guide rail, and the water in the plurality of constant temperature water tanks is maintained at different water temperatures;

[0009] A material rack is provided near one end of the transverse guide rail, and the material rack is provided with a plurality of material placement areas, and the plurality of material placement areas can be used to place a plurality of pieces of reaction materials;

[0010] a material rack moving module, movably disposed on the frame, and the first driving member can drive the material rack moving module to slide along the transverse guide rail; the material rack moving module includes a longitudinal guide rail, a second driving member, and a grasping manipulator, the longitudinal guide rail is disposed perpendicular to the transverse guide rail, and the second driving member can drive the grasping manipulator to lift and slide along the longitudinal guide rail;

[0011] The material rack is grabbed by the grabbing manipulator, so that the multiple pieces of the reaction materials on the material rack are circulated between the multiple constant temperature water tanks with different water temperatures.

[0012] Furthermore, the plurality of material placement areas are spaced apart from each other, and when a plurality of pieces of the reaction material are placed in the material placement area, the plurality of pieces of the reaction material are spaced apart from each other;

[0013] When the material rack with the plurality of pieces of the reaction materials placed thereon is placed in the constant temperature water tank, the water in the constant temperature water tank contacts all side walls of the reaction materials and performs heat transfer.

[0014] Furthermore, the material rack includes a bottom support, a peripheral frame and a plurality of U-shaped brackets;

[0015] A plurality of supporting steel wires are sequentially provided on the bottom support, and a plurality of pieces of the reaction material are supported on the supporting steel wires;

[0016] The outer frame is arranged on the bottom bracket and is fixedly connected to the bottom bracket; the top of the outer frame is provided with a rectangular frame, and an insertion opening is formed on both sides of the rectangular frame, and the insertion opening can be used to insert multiple pieces of the reaction material; a limiting steel wire is provided on both sides of the outer frame, and the limiting steel wire limits the lateral movement of the reaction material in the material rack;

[0017] A plurality of U-shaped brackets are arranged on the base and fixedly connected to the base; two rows of U-shaped brackets are provided on the base, and each row includes a plurality of U-shaped brackets arranged at intervals, and a material placement area is formed between two adjacent U-shaped brackets in the same row.

[0018] Furthermore, the gripping manipulator comprises four chucks and four clamping jaws, wherein one clamping jaw is disposed on the inner side of each chuck, and the clamping jaw can rotate relative to the chuck to switch between a clamping position and a loosening position;

[0019] The chuck is configured as an isosceles trapezoidal block, with one inclined surface facing upward and the other inclined surface facing downward;

[0020] When the grasping robot grasps the material rack, the four clamps penetrate into the outer frame, and the plurality of clamps rotate out from the bottom of the rectangular frame and clamp toward the rectangular frame.

[0021] Furthermore, the first driving member and the second driving member are both configured as motors, the first driving member drives the material rack moving module to slide along the transverse guide rail through a gear rack mechanism or a screw nut mechanism, and the second driving member drives the grasping robot to slide up and down along the longitudinal guide rail through a gear rack mechanism or a screw nut mechanism.

[0022] Furthermore, a water receiving tray is provided at the bottom of the multiple constant temperature water tanks and the bottom of the material rack, and the projections of the material rack and the multiple constant temperature water tanks from top to bottom onto the water receiving tray are within the area surrounded by the periphery of the water receiving tray.

[0023] Furthermore, the water receiving tray and the multiple constant temperature water tanks are provided with drainage pipes, and the multiple drainage pipes are interconnected, and the drainage pipes are provided with switch valves.

[0024] Furthermore, the plurality of constant temperature water tanks are each provided with an upward opening, and the grabbing manipulator can place the material rack into the constant temperature water tank through the opening;

[0025] A movable top cover is provided at the opening of the constant temperature water tank, and the movable top cover can seal the opening.

[0026] Furthermore, a control module is further included, and the first driving member, the second driving member and the plurality of constant temperature water tanks are electrically connected or communicatively connected to the control module;

[0027] A compressor is built into each of the plurality of constant temperature water tanks.

[0028] The present invention solves the technical problem by adopting a technical solution of providing a control method for a polymerase chain reaction device, which is applied to the above-mentioned polymerase chain reaction device and comprises the following steps:

[0029] S1. The first driving member drives the rack moving module to move above the rack along the transverse guide rail;

[0030] S2. A plurality of pieces of the reaction material are placed on the material rack, and the second driving member drives the grabbing manipulator to move downward along the longitudinal guide rail to grab the material rack;

[0031] S3, the second driving member drives the grabbing manipulator to move upward along the longitudinal guide rail to lift the material rack;

[0032] S4, the first driving member drives the rack moving module to move along the transverse guide rail to above the preset constant temperature water tank;

[0033] S5, the second drive only drives the grabbing manipulator to move downward along the longitudinal guide rail to place the material rack into the constant temperature water tank;

[0034] S6. The material moving module drives the material rack to rotate between the multiple constant temperature water tanks.

[0035] Compared with the prior art, the present invention has at least the following beneficial effects:

[0036] In the present invention, the polymerase chain reaction device includes multiple constant temperature water tanks, and the temperatures of the multiple constant temperature water tanks are maintained at different levels. The temperatures of the multiple constant temperature water tanks are the temperatures required for each stage of the polymerase chain reaction. According to the progress of each stage of the reaction, the grabbing robot places the material rack containing the reaction material in the corresponding constant temperature water tank. The temperature changes quickly, which can effectively improve the reaction efficiency, thereby improving the preparation efficiency of the standard product and increasing production capacity. It is suitable for large-scale PCR instruments.

[0037] In the present invention, more than ten pieces of reaction materials can be placed on each rack at intervals, and when the reaction materials are placed on the rack and placed in a constant temperature water tank, all sides of the reaction materials are in contact with the water in the constant temperature water tank, thereby increasing the heat conduction contact area, improving the heat conduction efficiency, and further improving the production efficiency of standard products.

[0038] In the present invention, the material rack is driven to rotate between a plurality of constant temperature water tanks by the first driving member and the second driving member, so the degree of automation is high and the overall structure is simple. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 Schematic diagram of the structure of the polymerase chain reaction device of the present invention;

[0040] Figure 2 for Figure 1 Structural diagram from another perspective;

[0041] Figure 3 This is a structural diagram of the material movement module;

[0042] Figure 4 for Figure 3 Structural diagram from another perspective;

[0043] Figure 5 It is a structural diagram of the material rack;

[0044] Figure 6 It is a schematic diagram of the structure in which the reaction materials are placed on the material rack;

[0045] Figure 7 This is a structural diagram of the material rack and reaction materials placed in a constant temperature water tank.

[0046] In the picture:

[0047] 1. Frame; 10. First drive member; 11. Transverse guide rail;

[0048] 2. Constant temperature water tank;

[0049] 3. Material rack; 31. Bottom support; 311. Support wire; 32. Outer frame; 321. Rectangular frame; 322. Insertion and placement port; 323. Limiting wire; 33. U-shaped bracket;

[0050] 4. Material rack moving module; 40. Second driving member; 41. Longitudinal guide rail; 42. Grasping manipulator; 421. Chuck; 422. Clamping claw;

[0051] 5. Drain tray; 50. Drain pipe; 51. On / off valve;

[0052] 6. Reaction materials. DETAILED DESCRIPTION

[0053] The following are specific embodiments of the present invention and the accompanying drawings to further describe the technical solutions of the present invention, but the present invention is not limited to these embodiments.

[0054] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, movement status, etc. between the various components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0055] In addition, in the present invention, descriptions such as "first," "second," and "one" are for descriptive purposes only and should not be understood to indicate or imply their relative importance or implicitly specify the number of the technical features indicated. Therefore, a feature specified as "first" or "second" may explicitly or implicitly include at least one of such features. In the description of the present invention, "plurality" means at least two, such as two, three, etc., unless otherwise specifically defined.

[0056] In the present invention, unless otherwise specified or limited, the terms "connection" and "fixation" should be understood in a broad sense. For example, "fixation" can mean fixed connection, detachable connection, or integration; mechanical connection or electrical connection; direct connection or indirect connection through an intermediate medium; internal communication between two elements or interaction between two elements, unless otherwise specified. Those skilled in the art will be able to understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0057] In addition, the technical solutions between the various embodiments of the present invention can be combined with each other, but it must be based on the fact that ordinary technicians in this field can implement it. When the combination of technical solutions is mutually contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0058] Example 1:

[0059] like Figure 1-Figure 7 As shown, a polymerase chain reaction device of this embodiment includes: a frame 1, multiple constant temperature water tanks 2, a material rack 3, a material rack moving module 4 and a water receiving tray 5. Among them, the frame 1 is provided with a first driving member 10 and a transverse guide rail 11. Multiple constant temperature water tanks 2 are arranged in sequence along the length direction of the transverse guide rail 11, and the water in the multiple constant temperature water tanks 2 is maintained at different water temperatures. The material rack 3 is arranged near one end of the transverse guide rail 11, and multiple material placement areas are provided on the material rack 3, and multiple material placement areas can place multiple pieces of reaction materials 6. The material rack moving module 4 is movably arranged on the frame 1, and the first driving member 10 can drive the material rack moving module 4 to slide along the transverse guide rail 11; the material rack moving module 4 includes a longitudinal guide rail 41, a second driving member 40 and a grabbing manipulator 42, the longitudinal guide rail 41 is arranged perpendicular to the transverse guide rail 11, and the second driving member 40 can drive the grabbing manipulator 42 to rise and fall and slide along the longitudinal guide rail 41. During actual use, the material rack 3 is grabbed by the grabbing manipulator 42 , so that the multiple pieces of reaction materials 6 on the material rack 3 are circulated between multiple constant temperature water tanks 2 with different water temperatures.

[0060] In this embodiment, the polymerase chain reaction device includes multiple constant temperature water tanks 2, and the temperatures of the multiple constant temperature water tanks 2 are maintained at different levels. The temperatures of the multiple constant temperature water tanks 2 are the temperatures required for each stage of the polymerase chain reaction. According to the progress of each stage of the reaction, the grabbing robot 42 places the material rack 3 containing the reaction material 6 in the corresponding constant temperature water tank 2. The temperature changes quickly, which can effectively improve the reaction efficiency, thereby improving the preparation efficiency of the standard product and increasing production capacity, and is suitable for large-scale PCR instruments.

[0061] A water collecting tray 5 is provided at the bottom of multiple constant temperature water tanks 2 and the bottom of the material rack 3. When the grabbing robot 42 grabs the material rack 3 and circulates between the various constant temperature water tanks 2, water is likely to drip. The setting of the water collecting tray 5 can collect the dripping water and prevent the dripping water from flowing everywhere.

[0062] Specifically, the projections of the material rack 3 and multiple constant temperature water tanks 2 from top to bottom onto the water receiving tray 5 are within the area surrounded by the periphery of the water receiving tray 5. The water receiving tray 5 in this embodiment is rectangular, and its edges are folded upward to form a water receiving tray 5 with an opening facing upward. The edges of the water receiving tray 5 are arranged around the periphery of multiple constant temperature water tanks 2 and the material rack 3 to ensure that when the grabbing robot 42 grabs the material rack 3, all water falling from the material rack 3 can be collected in the water receiving tray 5 to prevent it from falling outside the water receiving tray 5.

[0063] Furthermore, drain pipes 50 are provided on the water tray 5 and the multiple thermostatic water tanks 2, and these multiple drain pipes 50 are interconnected. Connecting these multiple drain pipes 50 not only reduces the number and length of drain pipes 50 used, but also improves the cleanliness of the device. Drain pipes 50 are provided with on / off valves 51. Each drain pipe 50 can be equipped with an on / off valve 51 to precisely control the drainage of one or more thermostatic water tanks 2 and the water tray 5. Alternatively, a single on / off valve 51 can be provided to control the simultaneous drainage of multiple thermostatic water tanks 2 and the water tray 5.

[0064] The polymerase chain reaction device of this embodiment can be arranged as a whole on a work surface to facilitate the arrangement of the drain pipe 50 and other components.

[0065] like Figures 1-4 As shown, in this embodiment, the first drive member 10 and the second drive member 40 are both configured as motors. The first drive member 10 drives the material rack moving module 4 to slide along the transverse guide rail 11 through a gear rack mechanism or a screw-nut mechanism. Optionally, when the first drive member 10 drives the material rack moving module 4 to slide along the transverse guide rail 11 through the gear rack mechanism, a gear is provided on the output shaft of the first drive member 10, i.e., the motor, and a rack is provided on the transverse guide rail 11. The first drive member 10 drives the gear to rotate, thereby causing the rack to perform linear motion. When the first drive member 10 drives the material rack moving module 4 to slide along the transverse guide rail 11 through the screw-nut mechanism, the first drive member 10 drives the screw to rotate, and the nut slides along the screw, thereby causing the material rack moving module 4 to slide along the transverse guide rail 11. Since the gear rack mechanism and the screw-nut mechanism are common linear motion mechanisms in the prior art, they will not be described in detail here.

[0066] Similarly, the second driving member 40 also drives the grabbing robot 42 to move up and down and slide along the longitudinal guide rail 41 through a gear rack mechanism or a screw nut mechanism.

[0067] In actual use, the polymerase chain reaction device of this embodiment drives the material rack 3 to rotate between multiple constant temperature water tanks 2 through the first driving member 10 and the second driving member 40, with a high degree of automation and a simple overall structure.

[0068] like Figure 5-Figure 7 Combined with Figure 3 As shown, in this embodiment, multiple material placement areas are spaced apart. When multiple pieces of reaction material 6 are placed in the material placement areas, the multiple pieces of reaction material 6 are spaced apart. In this embodiment, at least ten pieces of reaction material 6 can be placed on the material rack, and the multiple pieces of reaction material 6 are spaced apart. When the material rack 3 containing multiple pieces of reaction material 6 is placed in the constant temperature water tank 2, the water in the constant temperature water tank 2 contacts all side walls of the reaction material 6, and heat is transferred.

[0069] Specifically, the material rack 3 of this embodiment includes a base 31 , an outer frame 32 and a plurality of U-shaped brackets 33 .

[0070] Multiple support wires 311 are sequentially provided on the base 31, supporting the multiple pieces of reaction material 6 on the support wires 311. Without the support wires 311, the bottom surface of the reaction material 6 would directly contact the base 31, reducing the contact area with the water in the water tank. However, the support wires 311 effectively increase the contact area between the bottom surface of the reaction material 6 and the water in the water tank, thereby improving heat transfer efficiency. In this embodiment, each row of reaction material 6 is supported by two side-by-side support wires 311.

[0071] The outer frame 32 is mounted on the base 31 and welded to the base 31 to form a single piece. A rectangular frame 321 is formed on the top of the outer frame 32. This rectangular frame 321 facilitates the grasping robot 42 in grasping the material rack 3. An insertion opening 322 is formed on each side of the rectangular frame 321. Multiple pieces of reaction material 6 can be inserted into the insertion opening 322. The top and sides of the insertion opening 322 are open, facilitating the insertion of the reaction material 6 into the material rack 3. Furthermore, a limiting wire 323 is provided on each side of the outer frame 32 to restrict lateral movement of the reaction material 6 within the material rack 3. One limiting wire 323 is provided on each side of the outer frame 32. One limiting wire 323 restricts lateral movement of one row of reaction material 6, while the other limiting wire 323 restricts lateral movement of another row of reaction material 6, ensuring structural stability.

[0072] Multiple U-shaped brackets 33 are mounted on and fixedly connected to the base 31. Two rows of U-shaped brackets 33 are arranged on the base 31, with each row comprising multiple U-shaped brackets 33 spaced apart. A material placement area is formed between two adjacent U-shaped brackets 33 in the same row. Similarly, the U-shaped brackets 33 separate the individual pieces of reaction material 6, maximizing contact between each piece of reaction material 6 and the water in the tank, thereby improving heat transfer efficiency.

[0073] In actual use, each rack 3 of this embodiment can accommodate more than ten spaced-apart reaction materials 6. When the reaction materials 6 are placed on the rack 3 and placed in the constant-temperature water bath 2, all sides of the reaction materials 6 are in contact with the water in the constant-temperature water bath 2, increasing the heat transfer contact area and efficiency, further improving the production efficiency of standard products. Furthermore, during use, the polymerase reaction device of this embodiment maintains the same efficiency in producing standard products per unit volume of reaction material 6. For example, when testing with 10 ml or 20 ml of reaction material in the reaction bag, the reaction efficiency is not reduced compared to a conventional PCR instrument.

[0074] The gripping manipulator 42 of this embodiment includes four chucks 421 and four gripping claws 422. Each chuck 421 is configured with a gripping claw 422 on its inner side. The gripping claw 422 can rotate relative to the chuck 421 to switch between a clamped position and a loosened position. In addition, the four chucks 421 cooperate with the four gripping claws 422 to clamp the material rack 3, effectively ensuring the stability of the gripping of the material rack 3. During the turnover process, there will be no shaking, thus preventing the reaction material 6 on the material rack 3 from falling. In actual use, when the gripping manipulator 42 grasps the material rack 3, the four chucks 421 penetrate into the outer frame 32, and the multiple gripping claws 422 rotate out from the bottom of the rectangular frame 321 and clamp toward the rectangular frame 321.

[0075] Among them, the chuck 421 is set as an isosceles trapezoidal block, and one of its inclined surfaces is set upward and the other inclined surface is set downward. When the chuck 421 penetrates into the rectangular frame 321 or detaches from the rectangular frame 321, the chuck 421 is set as an isosceles trapezoidal block, and the upper and lower inclined surfaces can play a guiding role, making it easier to introduce or detach from the rectangular frame 321.

[0076] In this embodiment, a plurality of constant temperature water tanks 2 are each provided with an upward opening, and the grabbing manipulator 42 can place the material rack 3 into the constant temperature water tank 2 through the opening. The upward opening is provided to facilitate the grabbing manipulator 42 to place the material rack 3 into the constant temperature water tank 2 and also facilitate the grabbing manipulator 42 to take the material rack 3 out. Furthermore, a movable top cover is provided at the opening at the top of the constant temperature water tank 2. The movable top cover can be set to automatically flip up to achieve the purpose of automatic opening. Of course, the movable top cover can also be set to slide.

[0077] Preferably, the polymerase chain reaction device of this embodiment also includes a control module, which includes a touch screen display, and the operator can control the operation through the touch screen display. Among them, the first driving member 10, the second driving member 40 and multiple constant temperature water tanks 2 are electrically connected or communicated with the control module so that the control module controls the first driving member 10, the second driving member 40 and the electronic components in the constant temperature water tank 2. A compressor is built into each of the multiple constant temperature water tanks 2. The compressor plays an important role in the constant temperature water tank 2. The constant temperature water tank 2 maintains a stable experimental or working environment temperature by precisely controlling the water temperature, and the compressor can transfer heat. In order to precisely control the temperature of each constant temperature water tank 2, each constant temperature water tank 2 of this embodiment is equipped with a temperature sensor. The constant temperature water tank 2 can be heated by water circulation, and the flow rate of its water circulation is adjustable. The temperature range of the constant temperature water tank 2 can reach -5 to 100 degrees Celsius, the internal circulation flow rate can reach 5 to 10L / min, and the production efficiency can reach 500ML / hour.

[0078] Example 2:

[0079] A control method for a polymerase chain reaction device in this embodiment is applied to the polymerase chain reaction device in the first embodiment. That is, the control method also embodies the working principle of the polymerase chain reaction device in the first embodiment. Specifically, the control method includes the following steps:

[0080] S1. The first driving member 10 drives the rack moving module 4 to move above the rack 3 along the transverse guide rail 11.

[0081] S2: Multiple pieces of reaction material 6 are placed on rack 3, capable of holding at least ten pieces at a time. Second driver 40 drives gripper arm 42 downward along longitudinal guide rail 41 to grasp rack 3. Specifically, as gripper arm 42 moves downward along the longitudinal rail, four grippers 421 penetrate the rectangular frame 321 at the top of rack 3. At this point, grippers 422 are not deployed. Once fully engaged, grippers 421 rotate and expand, securing rectangular frame 321 and ensuring stable gripping of rack 3.

[0082] S3. The second driving member 40 drives the grabbing manipulator 42 to move upward along the longitudinal guide rail 41 to lift the material rack 3. The height of the lifted material rack 3 should be higher than the height of the constant temperature water tank 2 to avoid interference between the material rack 3 and the constant temperature water tank 2 during the movement.

[0083] S4. The first driving member 10 drives the material rack moving module 4 to move along the transverse guide rail 11 to the top of the preset constant temperature water tank 2. The constant temperature water tank 2 reaches the top opening of the preset constant temperature water tank 2. The preset constant temperature water tank 2 is the temperature required for the first stage of the polymerase chain reaction.

[0084] S5 , the second drive only drives the grabbing manipulator 42 to move downward along the longitudinal guide rail 41 , placing the material rack 3 into the constant temperature water tank 2 , and the water in the constant temperature water tank 2 immerses all the reaction materials 6 on the material rack 3 .

[0085] S6. The material moving module drives the material rack 3 to rotate between multiple constant temperature water tanks 2. Specifically, since each stage of the polymerase chain reaction needs to be carried out at a different temperature, using different constant temperature water tanks 2 to meet the temperature required for each stage of the reaction can effectively improve the production efficiency of the standard product.

[0086] In this scheme, the polymerase chain reaction device can effectively improve the preparation efficiency of standards and has a high degree of automation.

Claims

1. A polymerase chain reaction device, characterized in that: include: a frame, wherein the frame is provided with a first driving member and a transverse guide rail; A plurality of constant temperature water tanks are arranged in sequence along the length direction of the transverse guide rail, and the water in the plurality of constant temperature water tanks is maintained at different water temperatures; A material rack is provided near one end of the transverse guide rail, and the material rack is provided with a plurality of material placement areas, and the plurality of material placement areas can be used to place a plurality of pieces of reaction materials; a material rack moving module, movably disposed on the frame, and the first driving member can drive the material rack moving module to slide along the transverse guide rail; the material rack moving module includes a longitudinal guide rail, a second driving member, and a grasping manipulator, the longitudinal guide rail is disposed perpendicular to the transverse guide rail, and the second driving member can drive the grasping manipulator to lift and slide along the longitudinal guide rail; The material rack is grabbed by the grabbing manipulator, so that the multiple pieces of the reaction materials on the material rack are circulated between the multiple constant temperature water tanks with different water temperatures.

2. The polymerase chain reaction device according to claim 1, characterized in that The plurality of material placement areas are spaced apart from each other. When a plurality of pieces of reaction materials are placed in the material placement areas, the plurality of pieces of reaction materials are spaced apart from each other. When the material rack with the plurality of pieces of the reaction materials placed thereon is placed in the constant temperature water tank, the water in the constant temperature water tank contacts all side walls of the reaction materials and performs heat transfer.

3. The polymerase chain reaction device according to claim 1 or 2, characterized in that The material rack includes a bottom support, a peripheral frame and a plurality of U-shaped brackets; A plurality of supporting steel wires are sequentially provided on the bottom support, and a plurality of pieces of the reaction material are supported on the supporting steel wires; The outer frame is arranged on the bottom bracket and is fixedly connected to the bottom bracket; the top of the outer frame is provided with a rectangular frame, and an insertion opening is formed on both sides of the rectangular frame, and the insertion opening can be used to insert multiple pieces of the reaction material; a limiting steel wire is provided on both sides of the outer frame, and the limiting steel wire limits the lateral movement of the reaction material in the material rack; A plurality of U-shaped brackets are arranged on the base and fixedly connected to the base; two rows of U-shaped brackets are provided on the base, and each row includes a plurality of U-shaped brackets arranged at intervals, and a material placement area is formed between two adjacent U-shaped brackets in the same row.

4. The polymerase chain reaction device according to claim 3, characterized in that The gripping manipulator comprises four chucks and four clamping jaws, wherein a clamping jaw is disposed on the inner side of each chuck, and the clamping jaw can rotate relative to the chuck to switch between a clamping position and a loosening position; The chuck is configured as an isosceles trapezoidal block, with one inclined surface facing upward and the other inclined surface facing downward; When the grasping robot grasps the material rack, the four clamps penetrate into the outer frame, and the plurality of clamps rotate out from the bottom of the rectangular frame and clamp toward the rectangular frame.

5. The polymerase chain reaction device according to claim 1, characterized in that The first driving member and the second driving member are both configured as motors. The first driving member drives the material rack moving module to slide along the transverse guide rail through a gear rack mechanism or a screw nut mechanism, and the second driving member drives the grasping robot to slide up and down along the longitudinal guide rail through a gear rack mechanism or a screw nut mechanism.

6. The polymerase chain reaction device according to claim 1, characterized in that A water receiving tray is provided at the bottom of the multiple constant temperature water tanks and the bottom of the material rack. The projections of the material rack and the multiple constant temperature water tanks from top to bottom onto the water receiving tray are within the area surrounded by the periphery of the water receiving tray.

7. The polymerase chain reaction device according to claim 6, characterized in that The water receiving tray and the multiple constant temperature water tanks are all provided with drainage pipes, and the multiple drainage pipes are interconnected. The drainage pipes are provided with switch valves.

8. The polymerase chain reaction device according to claim 1, characterized in that The plurality of constant temperature water tanks are each provided with an upward opening, and the grabbing manipulator can place the material rack into the constant temperature water tank through the opening; A movable top cover is provided at the opening of the constant temperature water tank, and the movable top cover can seal the opening.

9. The polymerase chain reaction device according to claim 1, characterized in that It also includes a control module, and the first driving member, the second driving member and the plurality of constant temperature water tanks are electrically connected or communicatively connected to the control module; A compressor is built into each of the plurality of constant temperature water tanks.

10. A method for controlling a polymerase chain reaction device, applied to the polymerase chain reaction device according to any one of claims 1 to 9, characterized in that: Including steps: S1. The first driving member drives the rack moving module to move above the rack along the transverse guide rail; S2. A plurality of pieces of the reaction material are placed on the material rack, and the second driving member drives the grabbing manipulator to move downward along the longitudinal guide rail to grab the material rack; S3, the second driving member drives the grabbing manipulator to move upward along the longitudinal guide rail to lift the material rack; S4, the first driving member drives the rack moving module to move along the transverse guide rail to above the preset constant temperature water tank; S5, the second drive only drives the grabbing manipulator to move downward along the longitudinal guide rail to place the material rack into the constant temperature water tank; S6. The material moving module drives the material rack to rotate between the multiple constant temperature water tanks.

Images