Sample pretreatment equipment with dispersing and concentrating functions

Abstract

The invention discloses sample pretreatment equipment with dispersing and concentrating functions, and relates to the technical field of sample detection.The sample pretreatment equipment comprises a shell, and a sample injection unit, a sample treatment unit, a sampling unit and a detection unit are arranged in the shell; compared with existing sample pretreatment equipment, the sample pretreatment equipment has the advantages that automatic operation of sample pretreatment is realized through cooperative work of a plurality of moving mechanisms and functional components, and the sample pretreatment equipment comprises the steps of stirring, dispersing, sample concentrating and the like; through the sealing assembly, on one hand, the cleaning liquid is prevented from splashing to the external environment, and on the other hand, the stirring rod can be wiped through the sealing assembly, so that the cleaning liquid is prevented from entering a next to-be-stirred detection sample along with the stirring rod; the filtering mechanism and the concentrating mechanism both have an automatic cleaning function, so that the working efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of sample detection, and in particular to a sample pre-processing device with dispersing and concentrating functions. Background Art

[0002] In the fields of chemistry, biology, and environmental monitoring, sample pretreatment is one of the key steps in detection and analysis. Its purpose is to extract target substances (such as pesticide residues, mycotoxins, etc.) from complex samples for subsequent testing.

[0003] For example, the patents "CN107817144A Pesticide Residue Detection Pretreatment Device" and "CN213456336U Automated Pretreatment Equipment for Detecting Animal Residues" respectively disclose a method for sample pretreatment. However, traditional sample pretreatment equipment usually requires manual intervention in the process of stirring and dispersing samples and concentrating samples, and cannot achieve full-process automated operation, resulting in low work efficiency; in addition, in the cleaning link of the stirring component, in order to avoid splashing of the cleaning liquid, a cover or baffle is usually used to block the cleaning barrel, but this design cannot adapt to changes in the diameter of the stirring rod, and is prone to poor sealing, causing overflow of the cleaning liquid, thereby affecting the accuracy of subsequent sample processing; finally, in the sample filtering and sample concentration links, due to the complexity of the filtering mechanism and the concentration mechanism, traditional sample pretreatment equipment usually relies on manual cleaning. This operation method is not only inefficient, but also easily causes equipment contamination or sample loss, further increasing the burden on staff. Summary of the Invention

[0004] The object of the present invention is to provide a sample pre-processing device with dispersion and concentration functions to solve the problems raised in the prior art.

[0005] To achieve the above-mentioned objectives, the present invention provides the following technical solutions: a sample pretreatment device with a dispersion and concentration function, the sample pretreatment device comprising a shell, in which a sample injection unit, a sample processing unit, a sampling unit and a detection unit are arranged, the sample injection unit comprising a conveying device, the sample processing unit comprising a first movable mechanism and a second movable mechanism, the first movable mechanism being provided with a liquid adding component, the second movable mechanism being provided with a stirring component, the sampling unit comprising a third movable mechanism, a fourth movable mechanism and a fifth movable mechanism, the third movable mechanism being provided with a liquid extraction mechanism; the fourth movable mechanism being provided with a fork, the fifth movable mechanism being provided with a filtering mechanism, the detection unit comprising a detection table, the detection table being provided with a reagent bottle, a mixing mechanism, a cleaning tank and a concentration mechanism.

[0006] The working process of the present invention is as follows: the sample to be tested is placed in the test cup, the test cup is controlled by the conveying device to move in sequence to the bottom of the liquid adding component and the stirring component, the solvent is added to the test cup by the liquid adding component, and the sample to be tested is stirred by the stirring component (the functions realized by the liquid adding component and the stirring component belong to conventional technical means in this field, and the specific structure is not described). Through the cooperation of the liquid adding component and the stirring component, pesticide residues, fungal toxins and other substances in the sample to be tested are extracted. The fourth moving mechanism in the present invention is located at the end of the conveying device. After the test cup moves to the end of the conveying device, the fork is driven to move by the fourth moving mechanism, and the test cup is moved to the bottom of the filtering mechanism by the fork. The supernatant in the test cup is filtered out by the filtering mechanism. Finally, the staff can choose whether to concentrate the supernatant according to actual needs. When the supernatant does not need to be concentrated, the third moving mechanism first drives the liquid extraction mechanism to move to the reagent bottle to absorb the test liquid in the reagent bottle into the mixing mechanism. The supernatant is then sucked into the test cup by the liquid-lifting mechanism and moved to the mixing mechanism. The supernatant and the test liquid are mixed by the mixing mechanism so that subsequent staff can detect the concentrations of substances such as pesticide residues and mycotoxins in the supernatant. When the supernatant needs to be concentrated, the staff can first move the supernatant in the test cup into the concentrating mechanism through the third moving mechanism and the liquid-lifting mechanism, and concentrate the supernatant through the concentrating mechanism. When the supernatant is concentrated, the staff can suck the test liquid in the reagent bottle into the mixing mechanism through the third moving mechanism and the liquid-lifting mechanism, and then suck the concentrated supernatant into the mixing mechanism through the third moving mechanism and the liquid-lifting mechanism, and mix the concentrated supernatant and the test liquid through the mixing mechanism. In addition, the present invention is also provided with a cleaning tank on the test table. Each time the liquid-lifting mechanism on the third moving mechanism sucks the supernatant or the test liquid, the liquid-lifting mechanism needs to be moved to the cleaning tank for cleaning to avoid cross-infection of samples.

[0007] Furthermore, the stirring assembly includes a stirring motor, and a frame and a stirring rod are sequentially arranged below the stirring motor. When the sample to be tested in the detection cup needs to be stirred, the stirring rod is moved into the detection cup by the second moving mechanism, and the stirring motor drives the stirring rod to stir the sample to be tested. A cleaning bucket is provided at the side end of the conveying device, and the cleaning bucket is located below the second moving mechanism. A drainage channel is provided at the lower end of the interior of the cleaning bucket, and a liquid inlet pipe is provided on the outside of the cleaning bucket, and the liquid inlet pipe is connected to the upper end of the interior of the cleaning bucket. The liquid inlet pipe in the present invention is connected to the external cleaning system. The drainage channel is connected to the waste liquid recovery system. When the stirring of the sample to be tested in the test cup is completed, the stirring rod is moved into the cleaning barrel by the second moving mechanism, and then the stirring motor drives the stirring rod to rotate in the cleaning barrel, and the cleaning liquid is continuously transported into the cleaning barrel through the external cleaning system. The cleaning effect of the stirring rod is ensured by the above technical scheme. Finally, the present invention is provided with a sealing assembly inside the cleaning barrel. The sealing assembly is located above the outlet end of the liquid inlet pipe, and the lower end environment inside the cleaning barrel is separated from the outside world by the sealing assembly, so as to prevent the cleaning liquid from splashing into the external environment when the stirring rod rotates.

[0008] Furthermore, the sealing assembly includes a first mounting seat, which is detachably mounted on the upper inner end of the cleaning barrel by bolts, and a rectangular hole is provided at the middle position of the first mounting seat, and two sets of fixing frames are relatively provided at both ends of the inner portion of the rectangular hole, and each set of fixing frames is connected to the first mounting seat by a compression spring rod, and a set of electromagnets are provided on the side away from each other of the two sets of fixing frames, and a set of permanent magnets are provided on each set of fixing frames, and a set of cleaning cotton is provided inside the two sets of fixing frames. When the sealing assembly of the present invention is not working, the two sets of cleaning cottons are in a state of being away from each other. When the stirring rod to be cleaned is moved into the cleaning barrel, the two sets of electromagnets drive the two sets of fixing frames to approach each other. At this time, the two sets of cleaning cottons will fit each other and clamp the stirring rod. The two sets of fixing frames separate the lower end environment inside the cleaning barrel from the outside world, so as to prevent the cleaning liquid from splashing into the external environment when the stirring rod is cleaned. Compared with the current direct arrangement of cover plates, baffles and other structures to prevent the cleaning liquid from splashing, the present invention does not need to consider the diameter change of the stirring rod, thereby avoiding the phenomenon of loose sealing when the diameter of the stirring rod changes. Finally, after the cleaning of the stirring rod is completed, the staff can use the second moving mechanism to drive the stirring rod to perform reciprocating lifting and lowering motion between the two sets of cleaning cottons. The friction between the two sets of cleaning cottons and the stirring rod is used to perform a secondary cleaning of the stirring rod. On the one hand, it prevents the residue of the sample from being adhered to the surface of the stirring rod due to incomplete cleaning. On the other hand, the stirring rod is wiped by the two sets of cleaning cottons to prevent the cleaning liquid from entering the next test sample to be stirred with the stirring rod.

[0009] Furthermore, an annular tube is provided in the cleaning barrel, which is connected to the outlet end of the liquid inlet pipe. Several groups of drainage holes are provided at one end of the annular tube close to the center line of the cleaning barrel. When the stirring rod in the present invention rotates for cleaning in the cleaning barrel, several groups of drainage holes will release cleaning liquid with a certain pressure to flush the stirring rod. Compared with the current cleaning method, the cleaning effect of the present invention is better.

[0010] Furthermore, the filtering mechanism includes a first mounting tube, a filter plate, a second mounting tube and a third mounting seat, the third mounting seat is arranged at the working end of the fifth movable mechanism, the first mounting tube is arranged at the lower end of the third mounting seat, the second mounting tube is arranged inside the first mounting tube, and the filter plate is arranged inside the second mounting tube. When the fork moves the detection cup to the bottom of the filtering mechanism, the first mounting tube is extended into the detection cup through the fifth movable mechanism, and then the liquid extraction mechanism is moved to the top of the filtering mechanism through the third movable mechanism. The liquid extraction mechanism in the present invention adopts a pipette needle. After the liquid extraction mechanism moves to the top of the filtering mechanism, the liquid extraction mechanism is driven by the third movable mechanism to be inserted into the second mounting tube to extract the supernatant in the detection cup. The supernatant is filtered through the filter plate in the second mounting tube to prevent the sediment in the detection cup from being sucked away when the liquid extraction mechanism extracts the supernatant in the detection cup.

[0011] Furthermore, the third mounting seat is provided with a cavity at one end near the first mounting tube, and the cavity is connected to the external cleaning system. The lower end of the cavity is provided with an annular groove, and the cross-section of the annular groove is an inverted "eight" structure. The inner wall of the third mounting seat is provided with a plurality of groups of spray holes, and the plurality of groups of spray holes are all connected to the annular groove, and each group of spray holes is inclined downward. When the liquid lifting mechanism sucks away the supernatant in the detection cup, the staff can move the first mounting tube to the top of the detection cup through the fifth moving mechanism, and then transport the cleaning liquid into the cavity through the external cleaning system. At this time, the cleaning liquid will be ejected along the annular groove and the plurality of groups of spray holes. The cleaning liquid ejected from the annular groove cleans the outer wall of the first mounting tube, and the cleaning liquid ejected through the plurality of groups of spray holes cleans the inner wall of the second mounting tube and the filter plate. After cleaning is completed, the detection cup containing the cleaning liquid and sample sediment is moved back to the end of the transport device by the fork to facilitate the staff to dispose of the waste in the detection cup. Through the above technical solution, the present invention realizes the function of automatic cleaning of the filtering mechanism, thereby reducing the labor intensity of the staff.

[0012] Furthermore, the lower end of the first mounting tube is a truncated cone-shaped structure. When the cleaning liquid ejected from the annular groove cleans the outer wall of the first mounting tube, according to the Coanda effect, the cleaning liquid will flow along the outer wall surface of the lower end of the first mounting tube, thereby effectively improving the cleaning effect of the first mounting tube and avoiding the occurrence of cleaning dead corners. Finally, in the present invention, the first mounting tube and the third mounting seat are connected by a threaded connection, which makes it convenient for the staff to replace the filter plate at any time, thereby ensuring the filtering effect of the supernatant.

[0013] Furthermore, the concentration mechanism includes a concentration bottle and a water bath. The water bath is arranged below the test bench, the concentration bottle is arranged inside the water bath, the inlet end of the concentration bottle passes through the test bench, and a pure water heating element is arranged in the water bath.

[0014] Furthermore, a conduit is provided at the upper end of the concentration bottle, which is connected to the external cleaning system and the exhaust system. A waste liquid hole is provided at the lower end of the concentration bottle. A connecting seat is provided below the concentration bottle. A cylinder is provided inside the connecting seat. A sealing block is provided at the working end of the cylinder, which is adapted to the waste liquid hole. A waste liquid pipe is provided below the connecting seat, and the waste liquid pipe runs through the water bath.

[0015] Furthermore, a sealing ball is provided at the inlet end of the concentrate bottle, a through hole is provided at the middle position of the sealing ball, a second mounting seat is provided on the outside of the inlet end of the concentrate bottle, a reversing motor is provided inside the second mounting seat, and the working end of the reversing motor is connected to the sealing ball.

[0016] When the supernatant needs to be concentrated, the staff can insert the liquid extraction mechanism into the through hole on the sealing ball through the third moving mechanism, and then inject the supernatant into the concentration bottle, and then move the liquid extraction mechanism out of the concentration bottle through the third moving mechanism, and drive the sealing ball to rotate by the reversing motor until the through hole on the sealing ball no longer has an alignment area with the inlet end of the concentration bottle, and the concentration bottle is separated from the external environment by the sealing ball. Finally, the staff can turn on the exhaust system and the heating element, and heat the pure water in the water bath through the heating element, so that the temperature of the supernatant in the concentration bottle increases, and the environment in the concentration bottle is always in a negative pressure state through the exhaust system, thereby accelerating the concentration speed of the supernatant; when the concentration is completed, the staff can turn off the exhaust system and the heating element, and then drive the sealing ball to rotate by the reversing motor until the sealing ball The through hole on the bottle is aligned with the inlet end of the concentration bottle, and then the liquid extraction mechanism is inserted into the concentration bottle through the third moving mechanism, and the concentrated supernatant is taken away by the liquid extraction mechanism for subsequent use. Finally, if the staff needs to clean the concentration bottle, they only need to open the cylinder and the external cleaning system, move the sealing block out of the waste liquid hole through the cylinder, and fill the concentration bottle with cleaning liquid through the external cleaning system. At this time, the cleaning liquid will flow along the waste liquid hole, connecting seat and waste liquid pipe of the concentration bottle, so as to realize the cleaning of the concentration bottle and facilitate the subsequent concentration of the remaining supernatant. Through the above technical solution, the present invention can realize automatic concentration of the supernatant, and the concentration mechanism has the function of automatic cleaning, which avoids the need for special personnel to perform manual operation every time the supernatant is concentrated and cleaned, thereby significantly improving work efficiency.

[0017] Compared with the prior art, the beneficial effects of the present invention are: compared with the current sample pre-processing equipment, the present invention realizes the automated operation of sample pre-processing through the coordinated work of multiple moving mechanisms and functional components, including stirring, dispersing, concentrating samples and other steps, reduces manual intervention and improves work efficiency. In addition, the present invention is provided with a sealing component inside the cleaning barrel, which separates the lower end environment inside the cleaning barrel from the outside world through the sealing component, thereby preventing the cleaning liquid from splashing to the external environment when the stirring component is cleaning in the cleaning barrel. Compared with the current direct arrangement of cover plates, baffles and other structures to prevent the cleaning liquid from splashing, the present invention does not need to consider the diameter change of the stirring rod, thereby avoiding the phenomenon of loose sealing when the diameter of the stirring rod changes. At the same time, after the cleaning of the stirring rod is completed, the staff can drive the stirring rod to perform reciprocating lifting and lowering motion in the sealing component through the second moving mechanism. At this time, the sealing component will rub against the stirring rod, and the stirring rod can be Secondary cleaning is performed. On the one hand, it prevents sample residues from adhering to the surface of the stirring rod due to incomplete cleaning. On the other hand, the stirring rod can be wiped by the sealing component to prevent the cleaning liquid from entering the next test sample to be stirred with the stirring rod. The present invention is also provided with a filtering mechanism. Compared with the current method of directly setting a filter screen in a columnar tube, the filtering mechanism in the present invention includes a third mounting seat. The third mounting seat is provided with a cavity, an annular groove and a spray hole. When the filtering mechanism is used up, the cleaning liquid is transported into the cavity through an external cleaning system. At this time, the cleaning liquid will be ejected along the annular groove and several groups of spray holes to clean the area of the filtering mechanism that contacts the sample supernatant. Finally, the present invention is also provided with a concentrating mechanism. The supernatant is automatically concentrated by the concentrating mechanism. At the same time, the concentrating mechanism has the function of automatic cleaning, which avoids the need for special personnel to perform manual operation every time the supernatant is concentrated and cleaned, thereby significantly improving work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the appearance of the present invention;

[0019] Figure 2 This is a schematic diagram of the interior of the housing of the present invention from a first perspective;

[0020] Figure 3 A schematic diagram of the interior of the housing of the present invention from a second viewing angle;

[0021] Figure 4 This is a schematic structural diagram of the stirring assembly of the present invention;

[0022] Figure 5 This is a schematic diagram of the internal structure of the cleaning bucket of the present invention;

[0023] Figure 6 This is a schematic diagram of the internal structure of the first mounting base of the present invention;

[0024] Figure 7It is a schematic diagram of the structure of part of the sampling unit of the present invention;

[0025] Figure 8 Schematic cross-sectional view of the filtering mechanism of the present invention;

[0026] Figure 9 is a cross-sectional schematic diagram of a third mounting base of the present invention;

[0027] Figure 10 Schematic diagram of the detection unit structure of the present invention;

[0028] Figure 11 It is a structural schematic diagram of the concentration mechanism of the present invention;

[0029] Figure 12 This is a schematic diagram of the internal structure of the concentration bottle of the present invention.

[0030] In the figure: 1, housing; 2, testing cup; 3, conveying device; 4, first moving mechanism; 5, second moving mechanism; 51, stirring assembly; 511, stirring motor; 512, frame; 513, stirring rod; 52, cleaning barrel; 521, drainage channel; 522, annular pipe; 523, liquid inlet pipe; 524, first mounting base; 5241, fixing frame; 5242, electromagnet; 5243, cleaning cotton; 6, third moving mechanism; 7, testing table; 71, reagent bottle; 72, mixing mechanism; 7 3. Cleaning tank; 74. Concentrator bottle; 741. Second mounting seat; 742. Conduit; 743. Connecting seat; 7431. Cylinder; 7432. Sealing block; 744. Waste liquid pipe; 745. Sealing ball; 75. Water bath; 8. Fourth moving mechanism; 81. Fork; 9. Fifth moving mechanism; 91. Filter mechanism; 911. First mounting tube; 912. Filter plate; 913. Second mounting tube; 914. Third mounting seat; 9141. Cavity; 9142. Spray hole; 9143. Annular groove. DETAILED DESCRIPTION

[0031] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0032] Example: Figures 1-12As shown, the present invention provides a technical solution, a sample pretreatment device with a dispersion and concentration function, the sample pretreatment device includes a shell 1, and a sample injection unit, a sample processing unit, a sampling unit and a detection unit are arranged in the shell 1. The sample injection unit includes a conveying device 3, the sample processing unit includes a first moving mechanism 4 and a second moving mechanism 5, the first moving mechanism 4 is provided with a liquid adding component, and the second moving mechanism 5 is provided with a stirring component 51, the sampling unit includes a third moving mechanism 6, a fourth moving mechanism 8 and a fifth moving mechanism 9, the third moving mechanism 6 is provided with a liquid extraction mechanism; the fourth moving mechanism 8 is provided with a fork 81, and the fifth moving mechanism 9 is provided with a filtering mechanism 91, and the detection unit includes a detection table 7, and the detection table 7 is provided with a reagent bottle 71, a mixing mechanism 72, a cleaning tank 73 and a concentration mechanism.

[0033] The workflow of the present invention is as follows: a sample to be tested is placed in the test cup 2, and the test cup 2 is controlled by the conveying device 3 to move in sequence to the bottom of the liquid adding component and the stirring component 51. The solvent is added to the test cup 2 by the liquid adding component, and the sample to be tested is stirred by the stirring component 51 (the functions performed by the liquid adding component and the stirring component 51 belong to conventional technical means in the field, and the specific structure is not described). Through the cooperation of the liquid adding component and the stirring component 51, pesticide residues, mycotoxins and other substances in the sample to be tested are extracted. The fourth moving mechanism 8 of the present invention is located at the end of the conveying device 3. When the test cup 2 moves to the end of the conveying device 3, the fork 81 is driven by the fourth moving mechanism 8 to move, and the fork 81 moves the test cup 2 to the bottom of the filtering mechanism 91. The supernatant in the test cup 2 is filtered out by the filtering mechanism 91. Finally, the staff can choose whether to concentrate the supernatant according to actual needs. When the supernatant does not need to be concentrated, the third moving mechanism 6 first drives the liquid extraction mechanism to move to the reagent bottle 71 to absorb the test liquid in the reagent bottle 71. To the mixing mechanism 72, and then the supernatant in the detection cup 2 is sucked into the mixing mechanism 72 through the liquid lifting mechanism, and the supernatant and the detection liquid are mixed by the mixing mechanism 72, so that the subsequent staff can detect the concentration of substances such as pesticide residues and mycotoxins in the supernatant. When the supernatant needs to be concentrated, the staff can first move the supernatant in the detection cup 2 to the concentrating mechanism through the third moving mechanism 6 and the liquid lifting mechanism, and concentrate the supernatant through the concentrating mechanism. When the supernatant is concentrated, the staff can suck the detection liquid in the reagent bottle 71 into the mixing mechanism 72 through the third moving mechanism 6 and the liquid lifting mechanism, and then suck the concentrated supernatant into the mixing mechanism 72 through the third moving mechanism 6 and the liquid lifting mechanism, and mix the concentrated supernatant and the detection liquid through the mixing mechanism 72. In addition, the present invention is also provided with a cleaning tank 73 on the detection table 7. Each time the liquid lifting mechanism on the third moving mechanism 6 has sucked the supernatant or the detection liquid, the liquid lifting mechanism needs to be moved to the cleaning tank 73 for cleaning to avoid cross-infection of samples.

[0034] like Figure 2 、 Figure 4-Figure 6As shown, the stirring assembly 51 includes a stirring motor 511, and a frame 512 and a stirring rod 513 are sequentially arranged below the stirring motor 511. When the sample to be tested in the detection cup 2 needs to be stirred, the stirring rod 513 is moved into the detection cup 2 by the second moving mechanism 5, and the stirring motor 511 drives the stirring rod 513 to stir the sample to be tested. A cleaning bucket 52 is provided at the side end of the conveying device 3, and the cleaning bucket 52 is located below the second moving mechanism 5. A drainage channel 521 is provided at the lower end of the interior of the cleaning bucket 52, and a liquid inlet pipe 523 is provided on the outside of the cleaning bucket 52. The liquid inlet pipe 523 is connected to the upper end of the interior of the cleaning bucket 52, and the liquid inlet pipe 523 in the present invention is connected to the external cleaning system The drainage channel 521 is connected to the waste liquid recovery system. When the stirring of the sample to be tested in the test cup 2 is completed, the stirring rod 513 is moved to the cleaning barrel 52 by the second moving mechanism 5, and then the stirring motor 511 drives the stirring rod 513 to rotate in the cleaning barrel 52, and the cleaning liquid is continuously transported into the cleaning barrel 52 through the external cleaning system. The cleaning effect of the stirring rod 513 is ensured by the above technical scheme. Finally, the present invention is provided with a sealing component inside the cleaning barrel 52. The sealing component is located above the outlet end of the liquid inlet pipe 523, and the lower end environment inside the cleaning barrel 52 is separated from the outside world by the sealing component, so as to prevent the cleaning liquid from splashing into the external environment when the stirring rod 513 rotates.

[0035] like Figure 2 、 Figure 4-Figure 6 As shown, the sealing assembly includes a first mounting seat 524, which is detachably mounted on the upper end of the interior of the cleaning barrel 52 by means of bolts. A rectangular hole is provided at the middle position of the first mounting seat 524, and two sets of fixing frames 5241 are provided at opposite ends of the interior of the rectangular hole. Each set of fixing frames 5241 is connected to the first mounting seat 524 by a compression spring rod. A set of electromagnets 5242 are provided on the side away from each other of the two sets of fixing frames 5241, and a set of permanent magnets are provided on each set of fixing frames 5241. A set of cleaning cotton 5243 is provided inside the two sets of fixing frames 5241. When the sealing assembly of the present invention is not working, the two sets of cleaning cotton 5243 are in a state of being away from each other (such as Figure 6When the stirring rod 513 to be cleaned is moved into the cleaning barrel 52, the two sets of electromagnets 5242 drive the two sets of fixing frames 5241 to approach each other. At this time, the two sets of cleaning cotton 5243 will stick to each other and clamp the stirring rod 513. The lower end environment of the cleaning barrel 52 is separated from the outside world by the two sets of cleaning cotton 5243 and the two sets of fixing frames 5241, so as to prevent the cleaning liquid from splashing into the external environment when the stirring rod 513 is cleaned. Compared with the current direct arrangement of cover plates, shielding plates and other structures to prevent the cleaning liquid from splashing, the present invention does not need to consider the diameter change of the stirring rod 513, and avoids the stirring rod 513 from splashing. 3 changes, a loose seal occurs. Finally, after cleaning the stirring rod 513, the staff can use the second moving mechanism 5 to drive the stirring rod 513 to do a reciprocating lifting motion between the two sets of cleaning cotton 5243. The stirring rod 513 is cleaned twice by the friction between the two sets of cleaning cotton 5243 and the stirring rod 513. On the one hand, it prevents the sample residue from being adhered to the surface of the stirring rod 513 due to incomplete cleaning. On the other hand, the stirring rod 513 is wiped by the two sets of cleaning cotton 5243 to prevent the cleaning liquid from entering the next test sample to be stirred along with the stirring rod 513.

[0036] like Figure 5 As shown, an annular tube 522 is provided in the cleaning barrel 52, and the annular tube 522 is connected to the outlet end of the liquid inlet pipe 523. A plurality of drainage holes are provided at one end of the annular tube 522 close to the center line of the cleaning barrel 52. When the stirring rod 513 in the present invention rotates for cleaning in the cleaning barrel 52, the plurality of drainage holes will release cleaning liquid with a certain pressure to flush the stirring rod 513. Compared with the current cleaning method, the cleaning effect of the present invention is better.

[0037] like Figure 3 、 Figure 7-Figure 9 As shown, the filtering mechanism 91 includes a first mounting tube 911, a filter plate 912, a second mounting tube 913 and a third mounting seat 914. The third mounting seat 914 is arranged at the working end of the fifth moving mechanism 9, the first mounting tube 911 is arranged at the lower end of the third mounting seat 914, the second mounting tube 913 is arranged inside the first mounting tube 911, and the filter plate 912 is arranged inside the second mounting tube 913. When the fork 81 moves the detection cup 2 to the right below the filtering mechanism 91, the first mounting tube 91 is moved by the fifth moving mechanism 9. 1 is inserted into the detection cup 2. Then, the liquid extraction mechanism is moved to just above the filtering mechanism 91 by the third moving mechanism 6. The liquid extraction mechanism in the present invention adopts a pipette needle. After the liquid extraction mechanism is moved to just above the filtering mechanism 91, the third moving mechanism 6 drives the liquid extraction mechanism to be inserted into the second installation tube 913 to extract the supernatant in the detection cup 2. The supernatant is filtered by the filter plate 912 in the second installation tube 913 to prevent the sediment in the detection cup 2 from being sucked away when the liquid extraction mechanism extracts the supernatant in the detection cup 2.

[0038] like Figure 8-Figure 9 As shown, a cavity 9141 is provided at one end of the third mounting seat 914 close to the first mounting tube 911, and the cavity 9141 is connected to the external cleaning system. An annular groove 9143 is provided at the lower end of the cavity 9141, and the cross section of the annular groove 9143 is an inverted "eight"-shaped structure. A plurality of groups of spray holes 9142 are provided on the inner wall of the third mounting seat 914, and the plurality of groups of spray holes 9142 are connected to the annular groove 9143, and each group of spray holes 9142 is inclined downward. When the liquid lifting mechanism sucks away the supernatant in the detection cup 2, the staff can move the first mounting tube 911 to the top of the detection cup 2 through the fifth moving mechanism 9, and then clean the cavity 9 through the external cleaning system. 141, and the cleaning liquid is ejected along the annular groove 9143 and the plurality of groups of spray holes 9142. The outer wall of the first mounting tube 911 is cleaned by the cleaning liquid ejected from the annular groove 9143, and the inner wall of the second mounting tube 913 and the filter plate 912 are cleaned by the cleaning liquid ejected through the plurality of groups of spray holes 9142. When the cleaning is completed, the detection cup 2 containing the cleaning liquid and sample sediment is moved back to the end of the conveying device 3 by the fork 81 to facilitate the staff to dispose of the waste in the detection cup 2. Through the above technical solution, the present invention realizes the automatic cleaning function of the filter mechanism 91, thereby reducing the labor intensity of the staff.

[0039] like Figure 8-Figure 9 As shown, the lower end of the first mounting tube 911 is a truncated cone structure. When the cleaning liquid injected from the annular groove 9143 cleans the outer wall of the first mounting tube 911, according to the Coanda effect, the cleaning liquid will flow along the outer wall surface of the lower end of the first mounting tube 911, thereby effectively improving the cleaning effect of the first mounting tube 911 and avoiding the occurrence of cleaning dead corners. Finally, in the present invention, the first mounting tube 911 and the third mounting seat 914 are connected by a threaded connection, so that the staff can replace the filter plate 912 at any time, thereby ensuring the filtering effect of the supernatant.

[0040] like Figure 10-12 As shown, the concentration mechanism includes a concentration bottle 74 and a water bath 75. The water bath 75 is arranged below the detection table 7, and the concentration bottle 74 is arranged inside the water bath 75. The inlet end of the concentration bottle 74 passes through the detection table 7, and a pure water heating element is arranged in the water bath 75.

[0041] like Figure 10-12As shown, a conduit 742 is provided at the upper end of the concentration bottle 74, and the conduit 742 is connected to the external cleaning system and the exhaust system. A waste liquid hole is provided at the lower end of the concentration bottle 74, and a connecting seat 743 is provided below the concentration bottle 74. A cylinder 7431 is provided inside the connecting seat 743, and a sealing block 7432 is provided at the working end of the cylinder 7431. The sealing block 7432 is adapted to the waste liquid hole, and a waste liquid pipe 744 is provided below the connecting seat 743, and the waste liquid pipe 744 passes through the water bath 75.

[0042] like Figure 10-12 As shown, a sealing ball 745 is provided at the inlet end of the concentrate bottle 74, a through hole is provided at the middle position of the sealing ball 745, a second mounting seat 741 is provided on the outer side of the inlet end of the concentrate bottle 74, a reversing motor is provided inside the second mounting seat 741, and the working end of the reversing motor is connected to the sealing ball 745.

[0043] When the supernatant needs to be concentrated, the staff can insert the liquid extraction mechanism into the through hole on the sealing ball 745 through the third moving mechanism 6, and then inject the supernatant into the concentration bottle 74, and then move the liquid extraction mechanism out of the concentration bottle 74 through the third moving mechanism 6, and drive the sealing ball 745 to rotate by the reversing motor until the through hole on the sealing ball 745 no longer has an alignment area with the inlet end of the concentration bottle 74, and the concentration bottle 74 is separated from the external environment by the sealing ball 745. Finally, the staff can turn on the exhaust system and the heating element, and heat the pure water in the water bath 75 by the heating element, so that the temperature of the supernatant in the concentration bottle 74 increases, and the environment in the concentration bottle 74 is always in a negative pressure state through the exhaust system, thereby accelerating the concentration speed of the supernatant; when the concentration is completed, the staff can turn off the exhaust system and the heating element, and then drive the sealing ball 745 to rotate by the reversing motor until the sealing ball 74 The through hole on 5 is aligned with the inlet end of the concentration bottle 74, and then the liquid extraction mechanism is inserted into the concentration bottle 74 through the third moving mechanism 6, and the concentrated supernatant is taken away by the liquid extraction mechanism for subsequent use. Finally, if the staff needs to clean the concentration bottle 74, they only need to open the cylinder 7431 and the external cleaning system, move the sealing block 7432 out of the waste liquid hole through the cylinder 7431, and fill the concentration bottle 74 with cleaning liquid through the external cleaning system. At this time, the cleaning liquid will flow along the waste liquid hole, connecting seat 743 and waste liquid pipe 744 of the concentration bottle 74, so as to realize the cleaning of the concentration bottle 74, which is convenient for the subsequent concentration of the remaining supernatant. Through the above technical solution, the present invention can realize automatic concentration of the supernatant, and the concentration mechanism has the function of automatic cleaning, avoiding the need for special personnel to perform manual operation every time the supernatant is concentrated and cleaned, thereby significantly improving work efficiency.

[0044] The working principle of the present invention is as follows: before work, the sample to be tested is placed in the test cup 2, and then the test cup 2 is controlled by the conveying device 3 to move to the bottom of the liquid adding component and the stirring component 51 in sequence, and the solvent is added to the test cup 2 by the liquid adding component, and the sample to be tested is stirred by the stirring component 51. When the stirring of the sample to be tested in the test cup 2 is completed, the stirring rod 513 is moved to the cleaning barrel 52 by the second moving mechanism 5, and the lower end environment of the cleaning barrel 52 is separated from the outside world by the sealing component. Then, the stirring rod 513 is driven to rotate in the cleaning barrel 52 by the stirring motor 511, and the cleaning liquid is continuously conveyed into the cleaning barrel 52 by the external cleaning system to clean the stirring rod 513. When the test cup 2 moves to the end of the conveying device 3, the test cup 2 is moved to the bottom of the filtering mechanism 91 by the fork 81, and the supernatant in the test cup 2 is filtered out by the filtering mechanism 91. When the supernatant is not needed When the liquid is concentrated, the test liquid in the reagent bottle 71 is first sucked into the mixing mechanism 72 through the third moving mechanism 6 and the liquid lifting mechanism, and then the supernatant liquid after filtering in the test cup 2 is moved to the mixing mechanism 72, and the supernatant and the test liquid are mixed through the mixing mechanism 72, so that the subsequent staff can detect the concentration of pesticide residues, mycotoxins and other substances in the supernatant. When the supernatant needs to be concentrated, the staff first moves the supernatant in the test cup 2 to the concentrating mechanism through the third moving mechanism 6 and the liquid lifting mechanism, and concentrates the supernatant through the concentrating mechanism. When the supernatant is concentrated, the staff can suck the test liquid in the reagent bottle 71 into the mixing mechanism 72 through the third moving mechanism 6 and the liquid lifting mechanism, and then suck the concentrated supernatant into the mixing mechanism 72 through the third moving mechanism 6 and the liquid lifting mechanism, and mix the concentrated supernatant and the test liquid through the mixing mechanism 72.

[0045] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. The embodiments should therefore be considered illustrative and non-restrictive, and the scope of the invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be encompassed therein. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

Claims

1. A sample pre-processing device with dispersion and concentration functions, characterized by: The sample pre-processing device comprises a housing (1), wherein a sample introduction unit, a sample processing unit, a sampling unit and a detection unit are arranged in the housing (1), wherein the sample introduction unit comprises a conveying device (3), wherein the sample processing unit comprises a first moving mechanism (4) and a second moving mechanism (5), wherein the first moving mechanism (4) is provided with a liquid adding component, and the second moving mechanism (5) is provided with a stirring component (51), wherein the sampling unit comprises a third moving mechanism (6), a fourth moving mechanism (8) and a fifth moving mechanism (9), wherein the third moving mechanism (6) is provided with a liquid extracting mechanism; wherein the fourth moving mechanism (8) is provided with a fork (81), and wherein the fifth moving mechanism (9) is provided with a filtering mechanism (91), wherein the detection unit comprises a detection table (7), wherein the detection table (7) is provided with a reagent bottle (71), a mixing mechanism (72), a cleaning tank (73) and a concentrating mechanism, wherein both the filtering mechanism (91) and the concentrating mechanism have an automatic cleaning function.

2. The sample pre-processing device with dispersion and concentration functions according to claim 1, characterized in that: The stirring assembly (51) comprises a stirring motor (511), a frame (512) and a stirring rod (513) are sequentially arranged below the stirring motor (511), a cleaning bucket (52) is arranged at the side end of the conveying device (3), the cleaning bucket (52) is located below the second moving mechanism (5), a drainage channel (521) is arranged at the lower end of the interior of the cleaning bucket (52), a liquid inlet pipe (523) is arranged on the outside of the cleaning bucket (52), the liquid inlet pipe (523) is connected to the upper end of the cleaning bucket (52), and a sealing assembly is arranged inside the cleaning bucket (52), the sealing assembly is located above the outlet end of the liquid inlet pipe (523).

3. The sample pre-processing device with dispersion and concentration functions according to claim 2, characterized in that: The sealing assembly comprises a first mounting seat (524), a rectangular hole is provided at the middle position of the first mounting seat (524), two sets of fixing frames (5241) are provided at opposite ends of the interior of the rectangular hole, each set of fixing frames (5241) is connected to the first mounting seat (524) via a compression spring rod, a set of electromagnets (5242) are provided on the side of the two sets of fixing frames (5241) away from each other, a set of permanent magnets are provided on each set of fixing frames (5241), and a set of cleaning cotton (5243) is provided inside the two sets of fixing frames (5241).

4. The sample pre-processing device with dispersion and concentration functions according to claim 2, characterized in that: An annular tube (522) is provided in the cleaning barrel (52), the annular tube (522) being connected to the outlet end of the liquid inlet tube (523), and a plurality of groups of liquid drainage holes are provided at one end of the annular tube (522) close to the center line of the cleaning barrel (52).

5. The sample pre-processing device with dispersion and concentration functions according to claim 1, characterized in that: The filtering mechanism (91) comprises a first mounting tube (911), a filter plate (912), a second mounting tube (913) and a third mounting seat (914); the third mounting seat (914) is arranged at the working end of the fifth moving mechanism (9); the first mounting tube (911) is arranged at the lower end of the third mounting seat (914); the second mounting tube (913) is arranged inside the first mounting tube (911); and the filter plate (912) is arranged inside the second mounting tube (913).

6. The sample pre-processing device with dispersion and concentration functions according to claim 5, characterized in that: A cavity (9141) is provided at one end of the third mounting seat (914) close to the first mounting tube (911), and the cavity (9141) is connected to an external cleaning system. An annular groove (9143) is provided at the lower end of the cavity (9141), and the cross section of the annular groove (9143) is an inverted "eight"-shaped structure. A plurality of groups of spray holes (9142) are provided on the inner wall of the third mounting seat (914), and the plurality of groups of spray holes (9142) are all connected to the annular groove (9143), and each group of spray holes (9142) is inclined downward.

7. The sample pre-processing device with dispersion and concentration functions according to claim 6, characterized in that: The lower end of the first mounting tube (911) is a truncated cone-shaped structure, and the first mounting tube (911) is connected to the third mounting seat (914) via a threaded connection.

8. The sample pre-processing device with dispersion and concentration functions according to claim 1, characterized in that: The concentrating mechanism comprises a concentrating bottle (74) and a water bath (75), wherein the water bath (75) is arranged below the detection table (7), and the concentrating bottle (74) is arranged inside the water bath (75), and the inlet end of the concentrating bottle (74) passes through the detection table (7).

9. The sample pre-processing device with dispersion and concentration functions according to claim 8, characterized in that: The upper end of the concentration bottle (74) is provided with a conduit (742), and the conduit (742) is connected to the external cleaning system and the exhaust system. The lower end of the concentration bottle (74) is provided with a waste liquid hole. A connecting seat (743) is provided below the concentration bottle (74). A cylinder (7431) is provided inside the connecting seat (743). A sealing block (7432) is provided at the working end of the cylinder (7431). The sealing block (7432) is adapted to the waste liquid hole. A waste liquid pipe (744) is provided below the connecting seat (743), and the waste liquid pipe (744) passes through the water bath (75).

10. The sample pre-processing device with dispersion and concentration functions according to claim 9, characterized in that: A sealing ball (745) is provided at the inlet end of the concentration bottle (74), a through hole is provided at the middle position of the sealing ball (745), a second mounting seat (741) is provided on the outside of the inlet end of the concentration bottle (74), a reversing motor is provided inside the second mounting seat (741), and a working end of the reversing motor is connected to the sealing ball (745).

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