Cross-altitude chive seedling remote rejuvenation method and application thereof

Abstract

The invention belongs to the technical field of chive seedling rejuvenation, and particularly relates to a chive seedling cross-altitude remote rejuvenation method and application thereof. A method for rejuvenating cross-altitude chive seedlings in different places comprises the following steps that 1 / 3 leaves on the upper portions of the chive seedlings are cut off, root dipping treatment is conducted on the chive seedlings through a root system activating microbial agent, and then the treated seedlings are planted in a high-altitude planting area where a microbial agent is applied; in the planting period, a mixed solution of a salicylic acid solution, a monopotassium phosphate solution and a cytex solution is sprayed on leaf surfaces once every 30 days. According to the method, altitude gradient shuttle planting and microorganism-plant interaction regulation are creatively combined, chive resistance related genes are activated through high-altitude low-temperature stress, salicylic acid induction with precise time sequence is matched, and the specially designed complex microbial inoculant not only realizes functional complementation, but also can maintain the abundance of soil flora, so that the growth of chive resistance related genes is promoted, and the growth of chive resistance related genes is promoted. The stress resistance and the growth vigor of chive seedlings can be effectively improved, and the quality and the yield of the chive seedlings can be remarkably improved.

Description

Technical Field

[0001] The invention belongs to the technical field of shallot seedling rejuvenation, and particularly relates to a cross-altitude shallot seedling off-site rejuvenation method and application thereof. Background Art

[0002] Chives are an important specialty vegetable cultivated in southern my country. They are annual or perennial herbs of the genus Allium, family Amaryllidaceae, order Liliales. In southern China, they mainly include two species: shallot (Allium fistulosum L. var. caespitosum Makino) and shallot (Allium ascalonicum L.). They are used for fresh eating, processing, gardening, and medicinal purposes, and have unique edible and medicinal value. In southern my country, chives can be cultivated year-round. Vegetative propagation by pseudostem division is the primary method of industrial and commercial production, and therefore seedling quality directly impacts yield and economic returns. Guangxi, a major chive producing region, primarily uses stem division for transplanting, resulting in a high multiple cropping index and requiring 3-5 transplants annually. Chives have been cultivated in major producing areas for over 20 years. In recent years, large-scale cultivation has led to a significant problem of seedling degeneration due to long-term asexual propagation. This degeneration is primarily manifested by decreased root vitality, fewer tillers, and lower yields. This degradation of seedling quality has become a bottleneck hindering the sustainable and healthy development of the chive industry.

[0003] Seed propagation is also commonly used in northern my country to strengthen seedlings, generally with autumn sowing as the primary method. Chives' growth and development progresses through the following stages: seed germination, seedling development, tillering, and bolting. Plants are divided and transplanted 50 days after sowing, then harvested as needed. After overwintering, they flower and bolt in February and March, with seeds collected in April and May. However, seed propagation presents two major challenges: first, the seedling stage from germination to flowering is longer, and second, most chive varieties are not vernalized adequately in southern my country. Coupled with the low sunlight and high rainfall in spring, fertilization is hindered, making bolting, flowering, and the formation of plump seeds difficult, resulting in an extremely low seed germination rate.

[0004] Currently, seedling rejuvenation is often achieved by soaking them in chemical fungicides. While this method can improve transplant survival rates, it can also reduce soil urease activity and the abundance of beneficial bacteria such as Pseudomonas. More seriously, residual fungicides can increase malondialdehyde levels in chive leaves, leading to lipid peroxidation damage. This rejuvenation method, which sacrifices soil health, runs counter to the trend of green agriculture.

[0005] There is an urgent need to establish a chive seedling rejuvenation method that is suitable for chive production in the south and in line with the development trend of green agriculture.

[0006] The information disclosed in this background technology section is only intended to enhance understanding of the overall background of the invention and should not be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the Invention

[0007] The object of the present invention is to provide a cross-altitude off-site rejuvenation method for chive seedlings and its application, so as to solve the problems existing in the prior art in the background technology.

[0008] To achieve the above object, the present invention provides the following technical solutions: A method for rejuvenating chive seedlings at different altitudes comprises the following steps: Cut off the upper 1 / 3 leaves of the shallot seedlings and dip the roots of the shallot seedlings in a root activation agent, then plant the treated seedlings in a high-altitude planting area where microbial agents are applied; 15 days after planting, spray the leaves with a mixed solution of salicylic acid solution, potassium dihydrogen phosphate solution and seaweed solution, and repeat every 30 days, for a total of 2-3 sprayings.

[0009] Specifically, the altitude of the high-altitude planting area is not less than 600m, and the altitude of the area to be rejuvenated is not less than 400m.

[0010] Specifically, the root activation agent is prepared by mixing arbuscular mycorrhizal fungi, Trichoderma and Bacillus subtilis in a mass ratio of 2:1:2.

[0011] Specifically, the concentrations of the arbuscular mycorrhizal fungi, Trichoderma and Bacillus subtilis are 1×10 6 cfu / g, 1×10 7 spores / mL and 1×10 8 cfu / mL.

[0012] Specifically, the microbial agent is prepared by mixing Bacillus subtilis solution, Bacillus mucilaginosus solution, Bacillus megaterium solution and Bacillus licheniformis solution in a volume ratio of 8:3:3:5.

[0013] Specifically, the viable cell counts of the Bacillus subtilis solution, the Bacillus mucilaginosus solution, the Bacillus megaterium solution, and the Bacillus licheniformis solution are 1×10 9 cfu / mL, 8×10 7 cfu / mL, 3×10 7 cfu / mL and 5×10 7 cfu / mL.

[0014] Specifically, the dosage of the microbial agent is 10-20 kg / mu, and the application method is to mix the microbial agent with decomposed organic fertilizer in a ratio of 1:5 and then spread it on the soil surface, plow it into the soil, and the plowing depth is 15-20 cm.

[0015] Specifically, the concentration of the salicylic acid solution is 0.1-0.3 mmol / L, the concentration of the potassium dihydrogen phosphate solution is 0.05%, and the concentration of the algae extract is 0.1%.

[0016] Compared with the prior art, the present invention has the following beneficial effects: This invention innovatively combines altitude gradient shuttle planting with microbial-plant interaction regulation, activates chive resistance-related genes through high-altitude and low-temperature stress, and combines it with precisely timed salicylic acid induction. The specially designed composite bacterial agent not only achieves functional complementarity, but also maintains the abundance of soil microbial communities. This multi-dimensional synergistic rejuvenation strategy can not only effectively improve the stress resistance and growth potential of chive seedlings, but also significantly improve the quality and yield of chive seedlings. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 To achieve the rejuvenation effect of chive seedlings across different altitudes; Figure 2 This is the reproduction effect of shallot seedlings after being rejuvenated in a different location across altitudes.

[0018] Figure 1 Among them, the variety is white-headed onion; A is the main producing area of scallion in Sandu Town, Liujiang District, Liuzhou City, Guangxi, and B is Tonglian Township, Rongshui County, Liuzhou City, Guangxi. DETAILED DESCRIPTION

[0019] The following examples are provided to illustrate the present invention in detail, and the examples are intended to be a part of the present invention, rather than all of the examples. Example

[0020] A method for rejuvenating chive seedlings at different altitudes comprises the following steps: Cut off the upper 1 / 3 of the leaves of shallot seedlings and dip the roots in a root activation agent. Then plant the treated seedlings in a high-altitude planting area where a microbial agent is applied. Spray the leaves with a mixture of salicylic acid solution, potassium dihydrogen phosphate solution, and seaweed solution 15 days after planting, and then spray the leaves with a mixture of salicylic acid solution, potassium dihydrogen phosphate solution, and seaweed solution every 30 days. The altitude of the high-altitude planting area is not less than 600m, and the altitude of the area to be rejuvenated is not less than 400m; The root activation agent is prepared by mixing arbuscular mycorrhizal fungi, Trichoderma and Bacillus subtilis in a ratio of 2:1:2, and the concentrations of the arbuscular mycorrhizal fungi, Trichoderma and Bacillus subtilis are 1×10 6 cfu / g, 1×10 7 spores / mL and 1×10 8 cfu / mL; The microbial agent is prepared by mixing Bacillus subtilis liquid, Bacillus mucilaginosus liquid, Bacillus megaterium liquid and Bacillus licheniformis liquid in a volume ratio of 8:3:3:5; the number of viable bacteria in the Bacillus subtilis liquid, Bacillus mucilaginosus liquid, Bacillus megaterium liquid and Bacillus licheniformis liquid is 1×10 9 cfu / mL, 8×10 7 cfu / mL, 3×10 7 cfu / mL and 5×10 7 cfu / mL; The dosage of the microbial agent is 20 kg / mu, and the application method is to mix the microbial agent with decomposed organic fertilizer in a ratio of 1:5 and then spread it on the soil surface, plow it into the soil, and the plowing depth is 20 cm; the decomposed organic fertilizer is purchased from Maolinfeng Organic Fertilizer of Guangxi Jianye Fertilizer Co., Ltd., N+P2O5+K2O≥5.0%, organic matter≥30%, effective live bacteria count: 20 million / gram, fertilizer registration certificate number: Guinongfei (2023) No. 4239.

[0021] The concentration of the salicylic acid solution is 0.1-0.3 mmol / L, the concentration of the potassium dihydrogen phosphate solution is 0.05%, and the concentration of the algae extract is 0.1%.

[0022] Example 2: Effects of different treatments on chive seedlings grown at different altitudes Material The test material is white-headed onion, and the test will start in July 2023. 2. Test location Low altitude areas: Sandu Town, Liujiang District, Liuzhou City, Guangxi, 160m above sea level; High altitude area: Tonglian Township, Rongshui County, Liuzhou City, Guangxi, 900m above sea level.

[0023] 3 Test methods Select shallot seedlings with the same growth and treat them according to the method in Table 1, with 15m 2 , repeated three times.

[0024] Table 1 Details of different treatments serial number Place Specific operations CK Low altitude areas Routine management Process 1 Low altitude areas CK+ root activation agent root dipping treatment Process 2 Low altitude areas CK+ soil microbial agent Process 3 Low altitude areas Under CK conditions, spray the leaves with a mixture of salicylic acid solution (0.1mmol / L), 0.05% potassium dihydrogen phosphate solution and 0.1% seaweed solution once 15 days after planting, and then spray once every 30 days, for a total of 3 times. Process 4 high altitude areas Routine management Process 5 high altitude areas Treatment 4+root activation agent root dipping treatment Process 6 high altitude areas Treatment 4+ soil microbial agent Process 7 high altitude areas Under treatment 4, a mixture of salicylic acid solution (0.1 mmol / L), 0.05% potassium dihydrogen phosphate solution and 0.1% seaweed solution was sprayed on the leaves once 15 days after planting, and then sprayed once every 30 days, for a total of 3 times. Processing 8 high altitude areas Processing 4 + Processing 5 + Processing 6 + Processing 7 In Table 1, the root activation agent is prepared by mixing arbuscular mycorrhizal fungi, Trichoderma and Bacillus subtilis in a ratio of 2:1:2, and the concentrations of the arbuscular mycorrhizal fungi, Trichoderma and Bacillus subtilis are 1×10 6 cfu / g, 1×10 7 spores / mL and 1×10 8 cfu / mL; The microbial agent is prepared by mixing Bacillus subtilis liquid, Bacillus mucilaginosus liquid, Bacillus megaterium liquid and Bacillus licheniformis liquid in a volume ratio of 8:3:3:5, and the viable bacterial counts of the Bacillus subtilis liquid, Bacillus mucilaginosus liquid, Bacillus megaterium liquid and Bacillus licheniformis liquid are 1×10 9 cfu / mL, 8×10 7 cfu / mL, 3×10 7 cfu / mL and 5×10 7 cfu / mL.

[0025] 4 Experimental Results The effects of different treatments on chive seedlings were investigated during the commercial period. Each treatment was repeated 3 times, and 10 plants were investigated in each repeat. The average value was calculated. Figure 1-2 and Table 2-4.

[0026] Table 2 Effects of different treatments on the root system of shallot seedlings serial number Root length (cm) Number of root tips per plant <![CDATA[Root activity (mg.g -1 .h -1 )]]> Seedling index CK 13.2 8.57 0.38 0.0583 Process 1 15.3 12.87 0.55 0.0711 Process 2 14.8 13.78 0.41 0.0716 Process 3 13.6 11.42 0.36 0.0623 Process 4 15.2 14.17 0.67 0.0890 Process 5 17.4 16.78 0.84 0.0984 Process 6 16.9 16.34 0.73 0.102 Process 7 16.1 15.57 0.67 0.0937 Processing 8 18.7 19.21 0.81 0.107 As shown in Table 2, compared with conventional management (CK) at low altitude areas, the cross-altitude off-site rejuvenation method for chive seedlings provided by the present invention (Treatment 8) at high altitude areas can significantly improve the root length, number of root tips per plant, root activity, and seedling index of chive seedlings. Specifically, the root length of chive seedlings in Treatment 8 reached 18.7 cm, an increase of 3.5 cm compared with CK; the number of root tips per plant reached 19.21, an increase of 10.64 compared with CK; and the root activity was 0.81 mg.g-1.h-1, an increase of 0.43 mg.g-1 compared with CK. -1 .h -1 The seedling vigor index was also significantly higher than that of CK. These results indicate that the cross-altitude off-site rejuvenation method of chive seedlings of the present invention can effectively improve the growth potential and stress resistance of chive seedlings, providing strong support for the sustainable and healthy development of the chive industry.

[0027] Table 3 Effects of different treatments on the growth of shallot seedlings serial number Plant height (cm) Number of tillers Number of leaves per plant Leaf length (cm) Leaf diameter (cm) Pseudostem length (cm) Single plant weight (g) Equivalent yield per mu (kg) CK 32.0 11.7 5.67 21.21 0.35 6.56 6.45 970.07 Process 1 33.25 12.8 5.01 23.54 0.30 7.32 7.93 1064.30 Process 2 32.04 14.0 5.78 21.04 0.40 6.58 6.88 1156.53 Process 3 34.48 10.4 5.64 22.78 0.34 6.64 8.76 1075.16 Process 4 36.57 15.6 5.86 26.54 0.36 10.28 8.90 1475.36 Process 5 40.69 18.7 5.90 27.80 0.41 9.72 9.96 1508.92 Process 6 39.47 20.1 5.32 27.78 0.48 10.56 9.68 1555.70 Process 7 38.95 17.4 5.28 28.60 0.50 11.17 8.60 1617.28 Processing 8 42.41 21.11 5.78 29.07 0.53 12.08 10.26 1649.65 As shown in Table 3, compared with conventional management (CK) at low altitudes, the cross-altitude off-site rejuvenation method for chive seedlings provided by the present invention (Treatment 8) at high altitudes significantly improved chive seedling plant height, tiller number, number of leaves per plant, leaf length, leaf transverse diameter, pseudostem length, and plant weight. Specifically, chive seedlings in Treatment 8 achieved a plant height of 42.41 cm, an increase of 10.41 cm compared to the CK; a tiller number of 21.11, an increase of 9.41 compared to the CK; a single leaf number of 5.78, similar to but slightly higher than the CK; a leaf length of 29.07 cm, an increase of 7.86 cm compared to the CK; a leaf transverse diameter of 0.53 cm, an increase of 0.18 cm compared to the CK; a pseudostem length of 12.08 cm, an increase of 5.52 cm compared to the CK; and a plant weight of 10.26 g, an increase of 3.81 g compared to the CK. Meanwhile, the chive yield per mu in treatment 8 reached 1649.65 kg, a significant increase of 679.58 kg compared to CK. These results demonstrate that the cross-altitude chive seedling rejuvenation method of the present invention not only effectively enhances the growth potential and stress resistance of chive seedlings, but also significantly improves the quality and yield of chive seedlings, providing strong support for the sustainable and healthy development of the chive industry.

[0028] Table 4 Effects of different treatments on stress resistance of shallot seedlings serial number MDA (nmol / mg prot) POD (U / mg prot) SOD (U / mg prot) CAT (U / mg prot) Proline (μmol / g) CK 0.4203 8.68 25.70 1.52 28.86 Process 1 0.3790 9.11 27.82 0.66 24.31 Process 2 0.3425 12.33 26.04 0.86 19.94 Process 3 0.3578 10.04 27.69 1.17 20.45 Process 4 0.2355 11.27 27.17 2.07 14.22 Process 5 0.2372 11.75 24.84 1.81 15.47 Process 6 0.2878 12.78 33.37 1.83 14.91 Process 7 0.2419 11.36 32.19 0.98 13.25 Processing 8 0.2083 12.65 31.00 1.76 12.79 Table 4 shows that compared with conventional management (CK) in high-altitude areas, the MDA (malondialdehyde) content of chive seedlings in Treatment 8 was significantly reduced, reaching only 0.2083 nmol / mg prot, a decrease of 0.2120 nmol / mg prot compared with CK, indicating a low degree of membrane lipid peroxidation and less cell membrane damage. The activities of POD (peroxidase), SOD (superoxide dismutase), and CAT (catalase) reached 9.11 U / mg prot, 31.00 U / mg prot, and 1.76 U / mg prot, respectively, which were increased by 0.47 U / mg prot, 5.30 U / mg prot, and 0.24 U / mg prot, respectively, compared with CK. The increased activities of these enzymes help to scavenge reactive oxygen free radicals in cells and reduce oxidative damage. At the same time, the proline content of chive seedlings in Treatment 8 was 12.79 μmol / g, which was slightly higher than that in CK. However, under adverse conditions, the accumulation of proline helps to maintain cellular osmotic pressure and improve the stress resistance of seedlings. In summary, the cross-altitude off-site rejuvenation method of shallot seedlings of the present invention can significantly improve the stress resistance of shallot seedlings, and provide a strong guarantee for their cultivation in areas with different altitudes.

[0029] In summary, the cross-altitude off-site rejuvenation method for chive seedlings (Treatment 8) provided by the present invention in high-altitude areas not only significantly improved the growth indicators and yield of chive seedlings, but also effectively enhanced their stress resistance. This further demonstrates the effectiveness and superiority of the cross-altitude off-site rejuvenation method of chive seedlings provided by the present invention. Furthermore, this rejuvenation method is simple to operate, affordable, and readily applicable in chive production practices, potentially injecting new vitality into the sustainable and healthy development of the chive industry.

[0030] Example 3: Effect of replanting seedlings after rejuvenation 1. Materials and Methods The test material is white-headed onion, and the test will start in October 2023. 2. Test location Low altitude areas: Sandu Town, Liujiang District, Liuzhou City, Guangxi, 160m above sea level; 3 Test methods The seedlings rejuvenated to the commercial stage according to treatment 8 of Example 2 and the seedlings of CK were planted back into the main chive production area of Sandu Town, Liujiang District, Liuzhou City to compare the reproduction effects.

[0031] 4 Experimental Results See the results Figure 2 and Table 5.

[0032] Table 5 Effects of different treatments on the growth of shallot seedlings variety category Plant height (cm) Number of tillers Pseudostem length (cm) Single plant weight (g) Equivalent yield per mu (kg) White-headed onion Control seedlings 31.2 11.3 6.37 7.23 1037.64 Rejuvenate seedlings 34.9 20.4 7.09 11.12 1948.71 As can be seen from Table 5, compared with the control seedlings (CK seedlings), the seedlings treated with the cross-altitude off-site rejuvenation method for chive seedlings provided by the present invention showed significant growth advantages when replanted. Specifically, the plant height of the rejuvenated seedlings reached 34.9 cm, an increase of 3.7 cm over the control seedlings; the number of tillers reached 20.4, an increase of 9.1 over the control seedlings; the pseudostem length was 7.09 cm, an increase of 0.72 cm over the control seedlings; and the single plant weight was 11.12 g, an increase of 3.89 g over the control seedlings. At the same time, the per-acre yield of chives from the rejuvenated seedlings reached 1948.71 kg, an increase of 911.07 kg over the control seedlings, and the yield-increasing effect was extremely significant. These results show that chive seedlings treated with the rejuvenation method of the present invention can continue to maintain their growth potential and yield advantages when replanted, providing a strong guarantee for the sustained and efficient production of the chive industry.

[0033] Example 4: Rejuvenation effect of different shallot varieties on shallot seedlings at different altitudes 1. Materials and Methods The test materials are white-headed onion and shallot, and the test will start in October 2023. 2. Test location Low altitude areas: Xialing Village, Xixiangtang District, Nanning City, Guangxi, 120m above sea level; High altitude area: Baidong Village, Ziyuan County, Guilin City, Guangxi, 1000m above sea level.

[0034] 3 Test methods Select shallot seedlings with consistent growth and treat them according to the method in Table 5. Set up a test area for each variety without repetition.

[0035] Table 5 Details of different treatments serial number Place Specific operations CK Low altitude areas Routine management deal with high altitude areas Root activation agent root dipping treatment + soil microbial agent + foliar spraying once every 30 days with a mixture of salicylic acid solution (0.1mmol / L), 0.05% potassium dihydrogen phosphate solution and 0.1% seaweed solution In Table 5, the root activation agent is prepared by mixing arbuscular mycorrhizal fungi, Trichoderma and Bacillus subtilis in a ratio of 2:1:2, and the concentrations of the arbuscular mycorrhizal fungi, Trichoderma and Bacillus subtilis are 1×10 6 cfu / g, 1×10 7 spores / mL and 1×10 8 cfu / mL. The microbial agent is prepared by mixing Bacillus subtilis liquid, Bacillus mucilaginosus liquid, Bacillus megaterium liquid and Bacillus licheniformis liquid in a volume ratio of 8:3:3:5, and the viable cell counts of the Bacillus subtilis liquid, Bacillus mucilaginosus liquid, Bacillus megaterium liquid and Bacillus licheniformis liquid are 1×10 9 cfu / mL, 8×10 7 cfu / mL, 3×10 7 cfu / mL and 5×10 7 cfu / mL.

[0036] 4 Experimental Results The results are shown in Table 6.

[0037] Table 6 Rejuvenation effect of seedlings of different shallot varieties variety category Plant height (cm) Leaf length (cm) Number of tillers Pseudostem length (cm) Single plant weight (g) Equivalent yield per mu (kg) White-headed onion CK 35.8 27.6 13.1 6.9 7.2 1237.64 deal with 46.9 30.8 16.7 10.90 9.9 1925.47 AF132 CK 34.1 19.8 8.2 7.4 6.5 971.70 deal with 41.3 32.2 11.0 8.3 8.2 1318.29 AF31 CK 42.4 32.9 18.0 7.1 8.3 1464.36 deal with 52.5 41.0 16.0 8.3 17.9 1735.50 AF94 CK 44.6 35.7 9.6 8.2 7.7 1376.51 deal with 57.2 41.8 13.7 10.9 10.8 2081.09 As can be seen from Table 6, in high-altitude areas, the cross-altitude off-site rejuvenation method of shallot seedlings provided by the present invention was adopted. Not only the white-headed shallot variety showed a significant yield-increasing effect, but other shallot varieties such as AF132, AF31 and AF94 also showed good growth performance and yield-increasing potential. Specifically, for the AF132 variety, the seedling plant height of the treatment group reached 41.3 cm, an increase of 7.2 cm compared with the control group; the number of tillers reached 11.0, an increase of 2.8 compared with the control group; the equivalent yield per mu was 1318.29 kg, an increase of 346.59 kg compared with the control group. For the AF31 variety, the seedling plant height of the treatment group reached 52.5 cm, an increase of 10.1 cm compared with the control group; the number of tillers reached 16.0, an increase of 8.0 compared with the control group; the equivalent yield per mu was 1735.50 kg, an increase of 271.14 kg compared with the control group. For the AF94 variety, the seedling height of the treated group reached 57.2 cm, an increase of 12.6 cm compared with the control group; the number of tillers reached 13.7, an increase of 4.1 compared with the control group; the equivalent yield per mu was as high as 2081.09 kg, a significant increase of 704.58 kg compared with the control group.

[0038] These results further demonstrate the broad applicability and effectiveness of the cross-altitude off-site rejuvenation method for chive seedlings on various chive varieties. Chive seedlings treated with this method can maintain good growth and yield potential in high-altitude areas, providing strong technical support for diversified planting and efficient production in the chive industry. Furthermore, this rejuvenation method is simple to operate, affordable, and readily applicable in chive production practices, potentially injecting new impetus into the sustained and healthy development of the chive industry.

[0039] In summary, the cross-altitude off-site rejuvenation method of chive seedlings presented herein has demonstrated significant yield increases for various varieties grown at high altitudes, further demonstrating its broad applicability and effectiveness. This method is not only simple to operate and cost-effective, but also readily applicable to chive production practices, potentially injecting new vitality into the sustainable and healthy development of the chive industry.

[0040] The foregoing descriptions of specific exemplary embodiments of the present invention are for purposes of illustration and description. These descriptions are not intended to limit the invention to the precise forms disclosed, and it is apparent that many variations and modifications are possible in light of the foregoing teachings. The exemplary embodiments have been selected and described for the purpose of explaining the specific principles of the invention and their practical application, thereby enabling those skilled in the art to realize and utilize a variety of exemplary embodiments of the invention and various options and modifications. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A method for rejuvenating chive seedlings at different altitudes, characterized in that: The steps include: Cut off the upper 1 / 3 leaves of the shallot seedlings and dip the roots of the shallot seedlings in a root activation agent, then plant the treated seedlings in a high-altitude planting area where microbial agents are applied; 15 days after planting, spray the leaves with a mixed solution of salicylic acid solution, potassium dihydrogen phosphate solution and seaweed solution, and repeat every 30 days, for a total of 2-3 sprayings.

2. The method for rejuvenating shallot seedlings at different altitudes according to claim 1, characterized in that: The altitude of the high-altitude planting area is not less than 600m, and the altitude of the area to be rejuvenated is not less than 400m.

3. The method for rejuvenating shallot seedlings at different altitudes according to claim 1, characterized in that: The root activation bacterial agent is prepared by mixing arbuscular mycorrhizal fungi, Trichoderma and Bacillus subtilis in a mass ratio of 2:1:

2.

4. The method for rejuvenating shallot seedlings at different altitudes according to claim 3, characterized in that: The concentrations of the arbuscular mycorrhizal fungi, Trichoderma and Bacillus subtilis were 1×10 6 cfu / g, 1×10 7 spores / mL and 1×10 8 cfu / mL.

5. The method for rejuvenating shallot seedlings at different altitudes according to claim 1, characterized in that: The microbial agent is prepared by mixing Bacillus subtilis solution, Bacillus mucilaginosus solution, Bacillus megaterium solution and Bacillus licheniformis solution in a volume ratio of 8:3:3:

5.

6. The method for rejuvenating shallot seedlings at different altitudes according to claim 5, characterized in that: The viable bacterial counts of the Bacillus subtilis, Bacillus mucilaginosus, Bacillus megaterium and Bacillus licheniformis solutions are 1×10 9 cfu / mL, 8×10 7 cfu / mL, 3×10 7 cfu / mL and 5×10 7 cfu / mL.

7. The method for rejuvenating shallot seedlings at different altitudes according to claim 1, characterized in that: The dosage of the microbial agent is 10-20 kg / mu, and the application method is to mix the microbial agent with decomposed organic fertilizer in a ratio of 1:5 and then spread it on the soil surface, plow it into the soil, and the plowing depth is 15-20 cm.

8. The method for rejuvenating shallot seedlings at different altitudes according to claim 1, characterized in that: The concentration of the salicylic acid solution is 0.1-0.3 mmol / L, the concentration of the potassium dihydrogen phosphate solution is 0.05%, and the concentration of the algae extract is 0.1%.

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