Special microbial agent for root nematodes and preparation method thereof

Abstract

The invention discloses a special microbial agent for root nematodes. The special microbial agent for the root nematodes is prepared from the following components: 100 to 600 parts of abamectin, 200 to 500 parts of purpureocillium lilacinum, 300 to 700 parts of trichoderma longibrachiatum, 150 to 500 parts of monopotassium phosphate, 80 to 400 parts of carbamide and 100 to 350 parts of fish protein organic fertilizer. The invention also discloses a preparation method of the special microbial agent for root nematodes. The microbial agent prepared by mixing the abamectin, the purpureocillium lilacinum, the trichoderma longibrachiatum, the monopotassium phosphate, the carbamide and the fish protein organic fertilizer achieves the purpose of effectively killing or inhibiting root nematodes, can promote the development of crop root systems and improve the drought resistance and yield, and the fish protein organic fertilizer is rich in nutrient substances and has a good application prospect. Sufficient nutrients can be provided for crops, healthy growth of the crops is promoted, cell division and plant root growth are further promoted, the yield and quality of the crops can be remarkably improved by using the microbial agent, and economic benefits are further improved.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and in particular relates to a special microbial agent for root nematodes and a preparation method thereof. Background Art

[0002] Root-knot nematodes are also known as root-knot nematodes. Plant nematode diseases are caused by the invasion and parasitism of plant parasitic nematodes. Investigations have found that more than 3,000 plants or crops are affected by nematode diseases, including grain crops and vegetable crops. The symptoms are that the lateral roots and fibrous roots are increased compared to normal, and spherical or conical white nodules of varying sizes are formed on the fibrous roots of young roots. The aboveground parts of the affected plants grow short and slow, with abnormal leaf color, few fruits, low yields, and even cause premature death of the plants. This is because the root nematodes destroy the normal differentiation and physiological activities of the root tissue, resulting in a decrease in photosynthesis. The fruits are often deformed or unevenly colored. After the bead-like nematodes invade the plants, the normal growth and development of the plants are persecuted, thereby affecting the yield and quality. The amount of nematodes has decreased. At present, the commonly used method for nematode control is mainly chemical agents. However, chemical control can only be effective in a short period of time. Farmers need to apply pesticides to crops regularly. Chemical agents remain in the soil, causing damage to the soil microecological environment, affecting the survival and reproduction of beneficial microorganisms in the soil, and thus destroying the soil ecological balance. The agents enter the water body through rainwater erosion or irrigation water infiltration, causing pollution to surface water and groundwater, threatening the survival of aquatic organisms and human drinking water safety. Long-term reliance on chemical agents for nematode control will lead to the degradation of crop root function and reduce the crop's own disease resistance, making it more susceptible to nematodes and other diseases. In this regard, we proposed a special microbial agent for root nematodes and a preparation method thereof. Summary of the Invention

[0003] In view of the shortcomings of the existing technology, the present invention provides a microbial agent specifically for root nematodes and a preparation method thereof to solve the above technical problems.

[0004] To achieve the above object, the present invention provides the following technical solution: a special microbial agent for root nematodes is composed of the following components in parts: 100-600 parts of abamectin, 200-500 parts of purpurogenous fungi, 300-700 parts of long-branched Trichoderma, 150-500 parts of potassium dihydrogen phosphate, 80-400 parts of carbonamide, and 100-350 parts of fish protein organic fertilizer.

[0005] Preferably, the invention is specifically composed of the following components: 400 parts of abamectin, 240 parts of purpurogenous fungi, 400 parts of long-branched Trichoderma, 280 parts of potassium dihydrogen phosphate, 256 parts of carbonamide, and 200 parts of fish protein organic fertilizer.

[0006] Preferably, Streptomyces avermectin is selected and inoculated into a culture medium for suspension culture. The temperature is required to be 27°C-29°C and the culture time is 50h-60h. The bacteria are separated from the culture solution by a centrifuge to obtain bacterial powder. The bacterial powder is mixed with ethanol and stirred at a stirring speed of 60rpm for 1h. Impurities in the solution are removed by filtration to obtain an avermectin solution. Abamectin crystals are obtained by a solvent crystallization method. The solvent in the crystals is removed by drying to obtain avermectin.

[0007] Preferably, a PDA culture medium is prepared by adding water to potato extract, glucose, and agar powder to a fixed volume, and the strain of Pseudomonas lilacinus is inoculated into the prepared culture medium under sterile conditions. The inoculated culture medium is placed in a constant temperature incubator and cultured at a temperature and humidity of 28°C. The growth of the strain is regularly observed and the culture conditions are adjusted as needed. When the strain grows well on the culture medium, an expansion operation is performed to obtain a sufficient amount of bacteria. The expanded strain is extracted by a liquid fermentation method to obtain spore powder, and the Pseudomonas lilacinus is obtained by freeze-drying.

[0008] Preferably, peat, vermiculite, cow dung and mushroom residue are mixed, purified water is used to dissolve the culture medium raw materials, and the solution is added to a fermentation tank, the water in the fermentation tank is heated to 50°C, the long-branch Trichoderma species is inoculated into the fermentation tank, the preset temperature is 20°C-35°C, the pH value of the fermentation liquid is controlled at 5.5-6.5, the fermentation time is 72h-120h, and after the fermentation is completed, the Trichoderma is mixed with a wetting agent, a dispersant, and a disintegrant, and then granulated and dried in a fluidized bed to form water-dispersible granules to prepare the long-branch Trichoderma.

[0009] Preferably, potassium nitrate and phosphoric acid are mixed in a ratio of 2:1, water is added to the mixture to fully dissolve it, and the mixed solution is transferred to a reaction vessel, heated to 80°C-90°C and continuously stirred. After the reaction is completed, the solution is allowed to stand and cool, and potassium dihydrogen phosphate is separated using a centrifuge. The separated potassium dihydrogen phosphate is dried to form anhydrous crystals to obtain potassium dihydrogen phosphate.

[0010] Preferably, the purified ammonia and carbon dioxide are mixed in a molar ratio of 2.8-4.5 and introduced into a synthesis tower. The pressure in the tower is 13-25 MPa, the temperature is 180°C-200°C, and the residence time of the reaction materials is 25 min-40 min to obtain a carbonamide solution containing excess ammonia and ammonium carbamate. The urea solution after the ammonia and ammonium carbamate are separated is evaporated to 99.5% by reducing pressure and cooling, and then granulated in a granulation tower to obtain carbonamide.

[0011] Preferably, the discarded fish bones are dried and then ground into powder using a grinder. The fish bone powder is placed in a fermentation barrel and a fermentation agent is added. The fish bone powder is fully stirred and mixed. The fermentation time is 168 hours to 240 hours. After the fish bone powder is decomposed, it is aired until it becomes dry. After grinding, fine fertilizer powder is obtained to prepare fish protein organic fertilizer.

[0012] Preferably, the preparation method of the microbial agent for root nematodes comprises the following steps:

[0013] S1. Prepare the corresponding number of materials;

[0014] S2, heating potassium dihydrogen phosphate, carbonamide, and fish protein organic fertilizer in a stirring tank and uniformly mixing;

[0015] S3. Adding avermectin, purpurogenous spores, and long-branched Trichoderma inoculants to the preliminarily mixed nutrients, and stirring thoroughly with a stirrer to ensure that the inoculants and nutrients are fully mixed without agglomeration or stratification;

[0016] S4, placing the mixture into a fermentation tank, sealing it and fermenting it;

[0017] S5. After the fermentation is completed, the bacterial agent is filtered and dried to remove impurities and excess water;

[0018] S6. Seal the prepared bacterial agent and store it at low temperature.

[0019] Preferably, in step S3, the stirring speed is 80 rpm, the stirring time is 1.5 h, and in step S4, the temperature of the fermentation tank is set at 25° C.-37° C., and the fermentation time is 72 h-120 h.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The present application uses a microbial agent prepared by mixing abamectin, purpurogenous fungi, long-branched Trichoderma, potassium dihydrogen phosphate, carbonamide and fish protein organic fertilizer to achieve the purpose of effectively killing or inhibiting root-knot nematodes. Abamectin has the characteristics of broad spectrum, high efficiency, resistance to resistance, long-lasting effect, low toxicity and low residue. Purpurogenous fungi kill the eggs and adults of root-knot nematodes through parasitism and nutrient competition. When used in combination with abamectin, it can significantly improve the prevention and control effect. The beneficial bacteria in the microbial agent can activate, loosen and improve the soil aggregate structure, releasing large, medium and trace beneficial elements in the soil. Long-term use can reduce pesticide use, reduce drug and fertilizer damage, and make the soil healthier. Potassium dihydrogen phosphate, as a plant growth regulator, can promote the development of crop roots, improve drought resistance and yield. Fish protein organic fertilizer is rich in nutrients, can provide sufficient nutrients for crops, promote healthy crop growth, and further promote cell division and plant root growth. The use of microbial agents can significantly increase crop yield and quality, increase farmers' economic income, reduce production costs, and further improve economic benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a flow chart of the preparation method of this microbial agent. DETAILED DESCRIPTION

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0024] The invention provides a technical solution: a special microbial agent for root nematodes is composed of the following components in parts: 100-600 parts of abamectin, 200-500 parts of purpurogenous fungi, 300-700 parts of long-branched trichoderma, 150-500 parts of potassium dihydrogen phosphate, 80-400 parts of carbonamide, and 100-350 parts of fish protein organic fertilizer.

[0025] Furthermore, the invention is specifically composed of the following ingredients: 400 parts of abamectin, 240 parts of purpurogenous fungi, 400 parts of long-branched trichoderma, 280 parts of potassium dihydrogen phosphate, 256 parts of carbonamide, and 200 parts of fish protein organic fertilizer.

[0026] Furthermore, avermectin is a 16-membered macrolide compound. Streptomyces avermectin is selected and inoculated in a culture medium for suspension culture. The temperature of Streptomyces avermectin needs to select a starting strain with vigorous growth and active metabolism. The culture process is controlled under constant temperature conditions, which is required to be 27°C-29°C. Within this temperature range, the activity of avermectin synthase can be promoted to increase product yield. The culture time is 50h-60h to ensure that the bacteria fully grow and reach the maximum biomass. After the culture is completed, the fermentation broth is separated into solid and liquid by a centrifuge, and the bacteria and the culture liquid are separated to obtain bacterial powder. The bacterial powder is mixed with ethanol and stirred at a stirring speed of 60rpm to ensure that the bacterial powder is fully in contact with ethanol to promote the dissolution of avermectin, and to avoid excessive stirring that causes cell rupture and release of impurities. The stirring time is 1h. Impurities in the solution are removed by filtration to obtain an avermectin solution. Abamectin crystals are obtained by solvent crystallization. First, the solution needs to be heated to 70°C to ensure that avermectin is completely dissolved. The solution is cooled sequentially to reach a supersaturated state, and then allowed to stand to induce crystal precipitation. After the crystals are fully precipitated, the crystals are separated from the mother liquor by filtration. The separated crystals are placed in an oven and the solvent in the crystals is removed by drying to obtain avermectin. Abamectin is an important pesticide ingredient with multiple activities such as insecticide, acaricide, and nematodeicide. It has stomach poison and contact killing effects on mites and insects, but cannot kill eggs. Its main mechanism of action is to interfere with neurophysiological activities and stimulate the release of γ-aminobutyric acid, while γ-aminobutyric acid Acid has an inhibitory effect on the nerve conduction of arthropods, which causes adult mites, nymphs and insect larvae to show paralysis symptoms and eventually die after contact with avermectin. Abamectin is mainly used for the prevention and control of internal and external parasites of poultry and livestock and crop pests. Among crops, it can effectively prevent and control vegetable diamondback moth, beet armyworm, leaf armyworm, fruit tree aphids, red spiders, gall mites, as well as flower aphids, scale insects, thrips and other pests. When using, it must be carried out according to the recommended dosage and method to avoid excessive use or misuse.

[0027] Furthermore, Pseudomonas lilacinus is an important microorganism. Potato extract, glucose, and agar powder are added with water to a fixed volume to prepare a PDA culture medium. Fresh, pest-free potatoes are selected as raw materials, and after being thoroughly washed and peeled, they are cut into small pieces. Distilled water is added and boiled. During the boiling, constant stirring is required to ensure that the nutrients in the potatoes are released into the water. The potato residue is filtered out through gauze to obtain a clear potato extract. Glucose is added to the potato extract as a carbon source and agar powder is used as a coagulant. After fully stirring and mixing, the total volume is adjusted with distilled water. After the fixed volume is completed, the mixture is poured into a sterile culture dish and allowed to solidify naturally to prepare the PDA culture medium. Culture medium, inoculate the strain of Pseudomonas lilacinus into the prepared culture medium under sterile conditions, ensure that the strain is evenly distributed on the culture medium, place the inoculated culture medium in a constant temperature incubator, and culture it at a temperature and humidity of 28°C. Pseudomonas lilacinus grows more vigorously in a high humidity environment. Observe the growth of the strain regularly to evaluate its growth status, including but not limited to colony morphology, color, and edge characteristics. If there are signs of contamination on the surface of the culture medium or the strain grows abnormally, adjust the culture conditions or re-inoculate, adjust the culture conditions as needed, and when the strain grows well on the culture medium, perform expansion operations to obtain Sufficient amount of bacteria, expansion is to transfer a part of the vigorously growing colony to a larger capacity culture medium, the expanded strain is extracted by liquid fermentation method to make spore powder, and the purple spore fungus is obtained by freeze drying. Purple spore fungus can secrete protease and chitinase to dissolve nematode egg shells, effectively prevent and control nematodes, and reduce the damage caused by repeated crops. It can also directly infect active nematode larvae and female adults, reduce the vitality, motility and survival rate of nematodes, in addition to secreting biochemical enzymes, it can also secrete the toxic substance white ash bacteriostatin with nematode killing activity, which has a good inhibitory effect on a variety of bacteria, fungi, cancer cells and trypanosomes. Purple spore The rich metabolites produced by bacterial metabolism can be used as nutrients for crops to promote crop growth. The active substances similar to plant growth hormones produced can promote crop growth under low concentration conditions. Pseudomonas aeruginosa has low requirements for nutrients. It can not only grow in a variety of conventional culture media, but also in many common and low-cost natural substrates, such as rice husks, rice bran, corn stalks, coffee shells and other plant wastes, as well as food residues such as bean dregs, sugarcane bagasse and cassava residue, or in poultry and livestock manure. In agriculture, Pseudomonas aeruginosa is widely used to kill insects and prevent diseases, increase crop yield and quality, and can also be used for soil improvement to enhance soil fertility and organic matter content.

[0028] Furthermore, peat, vermiculite, cow dung and fungus residue are mixed and purified water is used to dissolve the culture medium raw materials. Peat becomes an ideal matrix material because of its good air permeability and water retention. Vermiculite has high cation exchange capacity and water absorption, which helps to regulate the humidity and structure of the culture medium. Cow dung is rich in organic matter and nutrients required by various microorganisms, which provides rich nutrients for the growth of long-branch Trichoderma. Fungus residue is a waste material after edible fungus cultivation, and its residual nutrients can help Trichoderma grow rapidly. During the dissolution process, it is necessary to stir continuously to ensure that the raw materials are fully mixed, and the solution is added to a fermentation tank. The water in the fermentation tank is heated to 50°C to activate the microbial activity in the culture medium and kill some harmful microorganisms. The long-branch Trichoderma species is inoculated into the fermentation tank. The preset temperature is 20°C-35°C. The pH value of the fermentation liquid is controlled at 5.5-6.5, which is conducive to the growth metabolism of Trichoderma and the formation of spores. During the fermentation process, the temperature, pH value, dissolved oxygen content of the fermentation liquid and the growth condition of Trichoderma need to be monitored regularly to ensure that the fermentation process is as scheduled. The fermentation is carried out as expected, and the fermentation time is 72h-120h. When it is observed that the Trichoderma in the fermentation liquid grows vigorously and the spores are well formed, the fermentation can be ended. After the fermentation is completed, the Trichoderma is mixed with a wetting agent, a dispersant, and a disintegrant. The mixed material is sent to a fluidized bed granulation dryer. The material is granulated by fluidization and excess water is removed to form a water-dispersible granule to prepare long-branch Trichoderma. Long-branch Trichoderma can resist pathogens through competition and heavy parasitism, and produce a variety of plant pathogens. Antagonistic biologically active substances can prevent diseases, prevent root rot, promote new roots, promote growth, promote growth, and prevent nematodes. They can decompose root rot pathogens, promote rapid healing of injured root tissues, secrete active substances to promote the formation of new roots, and strengthen old roots. They are suitable for areas with serious problems such as repeated cropping soil, compacted soil, and acidified soil. When used in combination with chemical fertilizers, compound fertilizers, and organic fertilizers, it can improve fertilizer utilization, including the production of antibiotics, nutrient competition, cell wall decomposing enzymes, and inducing plant resistance.

[0029] Furthermore, potassium dihydrogen phosphate is an important inorganic compound. Potassium nitrate and phosphoric acid are mixed in a ratio of 2:1. After water is added to the mixture and fully dissolved, the mixed solution is transferred to a reaction vessel, heated to 80°C-90°C and continuously stirred. After the reaction is completed, the solution is allowed to stand and cool. The potassium dihydrogen phosphate is separated using a centrifuge, and the separated potassium dihydrogen phosphate is dried to become anhydrous crystals to obtain potassium dihydrogen phosphate. Potassium dihydrogen phosphate can significantly promote the absorption of nitrogen and phosphorus by crops. By promoting the absorption of nitrogen and phosphorus, potassium dihydrogen phosphate helps crops take root and promotes flower bud differentiation, thereby promoting To increase crop yield, potassium is one of the key elements of photosynthesis. The potassium in potassium dihydrogen phosphate can effectively promote crop photosynthesis, promote the synthesis and transportation of carbohydrates, and provide sufficient energy and material basis for crop growth. Potassium dihydrogen phosphate can significantly improve the stress resistance of crops, including drought resistance, cold resistance, lodging resistance, and disease resistance. This is of great significance for reducing the adverse effects of environmental stress on crop growth and improving crop yield and quality. Under high temperature and drought conditions, potassium dihydrogen phosphate can regulate the water balance in crops, reduce transpiration intensity, and avoid excessive dehydration and drying of crops.

[0030] Furthermore, carbonamide is an organic compound composed of carbon, nitrogen, oxygen and hydrogen. Purified ammonia and carbon dioxide are mixed in a molar ratio of 2.8-4.5 and then fed into a synthesis tower. The pressure in the tower is 13MPa-25MPa and the temperature is 180℃-200℃. Such conditions can maximize the chemical reaction between ammonia and carbon dioxide. The residence time of the reaction materials is 25min-40min to ensure sufficient reaction. A carbonamide solution containing excess ammonia and ammonium carbamate is obtained. The urea solution after separating the ammonia and ammonium carbamate is then reduced in pressure and cooled. After evaporation to 99.5%, carbonamide is granulated in a granulation tower to produce carbonamide. Carbonamide has a nitrogen content of up to 46%, which is an indispensable fertilizer for base fertilizer and early growth of crops. It is mainly used for base fertilizer and topdressing, especially in the critical period of crop growth. It can quickly replenish nitrogen in the soil, promote crop root development, lush leaves, and full fruits, and improve crop yield and quality. Carbonamide has good water solubility and can be dissolved in water for foliar spraying. This method of use can directly provide nutrients to crop leaves, so as to quickly replenish crop nitrogen deficiency and promote crop growth.

[0031] Furthermore, fish protein organic fertilizer is a pure natural organic fertilizer. The discarded fish bones are carefully collected and cleaned to remove impurities and salt on the surface. The fish bones are sent to the drying equipment and slowly baked at low temperature to remove moisture to prevent the growth of microorganisms. After drying, the fish bone powder is grinded into fine fish bone powder with a grinder. The fish bone powder is placed in a fermentation barrel and a fermentation agent is added. These fermentation agents contain a variety of beneficial microorganisms, including lactic acid bacteria and yeast. After sufficient stirring and mixing, the microorganisms in the fermentation agent multiply rapidly in the fermentation barrel. Through biochemical reactions, the organic matter in the fish bone powder is decomposed into small molecules that are more easily absorbed and utilized by plants, and rich fish protein and bioactive substances are produced. The fermentation time is 168h-240h to ensure sufficient fermentation and avoid the loss of nutrients. After the fermentation is completed, the fish bone powder is transformed into a dark brown, loose texture, and The fragrant compost and fish bone meal are decomposed and then air-dried until they are dry. After grinding, fine fertilizer powder is obtained to make fish protein organic fertilizer. Fish protein organic fertilizer is rich in nitrogen, phosphorus, potassium and other nutrients, which are key nutrients required for plant growth. They can promote plant growth and development, improve plant health, improve soil structure, increase soil water retention and aeration, provide nutrients required by soil microorganisms, promote the reproduction of beneficial microorganisms, and improve soil ecosystems. Fish protein organic fertilizer contains bioactive substances such as amino acids and plant growth regulators, which can enhance plant resistance and improve plant adaptability to adverse environmental conditions such as pests and diseases, drought, and cold. Compared with chemical fertilizers, fish protein fertilizers release nutrients more slowly and have a longer duration of fertilizer effect, which can reduce the need for frequent topdressing and reduce fertilizer usage and cost.

[0032] Furthermore, the preparation method of the microbial agent specifically for root nematodes comprises the following steps:

[0033] S1. Prepare the corresponding number of materials;

[0034] S2. Potassium dihydrogen phosphate, carbonamide, and fish protein organic fertilizer are heated and uniformly mixed in a stirred tank. Heating helps to accelerate the dissolution and mixing speed of the materials. Some organic matter will undergo chemical reactions at high temperatures to generate intermediate products that are easily utilized by microorganisms. The speed and time must be controlled during the stirring process to ensure that the materials are evenly mixed and there are no large particles or lumps.

[0035] S3. Add avermectin, purpurogenous spores, and long-branched Trichoderma agents to the preliminarily mixed nutrients. Since microbial agents are sensitive to environmental conditions, they need to be added gently to avoid vigorous stirring that damages the bacterial structure. Use a stirrer to fully stir to ensure that the agents and nutrients are fully mixed without agglomeration or stratification.

[0036] S4. The mixture is placed in a fermentation tank, sealed to prevent contamination by external bacteria, and then fermented. During the fermentation process, microorganisms utilize nutrients to grow and reproduce, producing bioactive substances;

[0037] S5. After fermentation, the inoculum is filtered and dried to remove impurities and excess water. The filtration is to remove solid residues and unused raw material particles generated during the fermentation process. The drying is to improve the stability and shelf life of the inoculum.

[0038] S6. Seal the prepared microbial agent to prevent the entry of oxygen, moisture, and bacteria in the air, and store it at low temperature to further extend the shelf life of the microbial agent and maintain its biological activity. During storage, the status of the microbial agent must be checked regularly to ensure that it is free of mold, caking, and deterioration.

[0039] Furthermore, in step S3, the stirring speed is 80 rpm, which can ensure sufficient mixing of the microbial agent and the nutrients, avoid agglomeration or stratification, and also reduce mechanical damage to the microbial cells caused by excessive stirring. The stirring time is 1.5 h to ensure that all ingredients can be fully mixed so that the microbial agent can be evenly distributed in the nutrients. During the stirring process, it is necessary to observe whether the mixture has any abnormal phenomena, including excessive foaming, excessive temperature or uneven stirring. In step S4, the fermentation tank temperature is set at 25°C-37°C. This temperature range can ensure the normal reproduction of microorganisms and promote the production of biologically active metabolites. The fermentation time is 72h-120h.

[0040] Example 1

[0041] The preparation method of the microbial agent for root nematodes comprises the following steps:

[0042] S1. Prepare the following materials: 400 parts of avermectin, 240 parts of purpurogenous fungi, 400 parts of long-branched trichoderma, 280 parts of potassium dihydrogen phosphate, 256 parts of carbonamide, and 200 parts of fish protein organic fertilizer;

[0043] S2, heating potassium dihydrogen phosphate, carbonamide, and fish protein organic fertilizer in a stirring tank and uniformly mixing;

[0044] S3. Adding avermectin, purpurogenous spores, and long-branched Trichoderma inoculants to the preliminarily mixed nutrients, and stirring thoroughly with a stirrer to ensure that the inoculants and nutrients are fully mixed without agglomeration or stratification;

[0045] S4, placing the mixture into a fermentation tank, sealing it and fermenting it;

[0046] S5. After the fermentation is completed, the bacterial agent is filtered and dried to remove impurities and excess water;

[0047] S6. Seal the prepared bacterial agent and store it at low temperature.

[0048] Example 2

[0049] The preparation method of the microbial agent for root nematodes comprises the following steps:

[0050] S1. Prepare the following materials: 100 parts of avermectin, 200 parts of purpurogenous fungi, 300 parts of long-branched trichoderma, 150 parts of potassium dihydrogen phosphate, 80 parts of carbonamide, and 100 parts of fish protein organic fertilizer;

[0051] S2, heating potassium dihydrogen phosphate, carbonamide, and fish protein organic fertilizer in a stirring tank and uniformly mixing;

[0052] S3. Adding avermectin, purpurogenous spores, and long-branched Trichoderma inoculants to the preliminarily mixed nutrients, and stirring thoroughly with a stirrer to ensure that the inoculants and nutrients are fully mixed without agglomeration or stratification;

[0053] S4, placing the mixture into a fermentation tank, sealing it and fermenting it;

[0054] S5. After the fermentation is completed, the bacterial agent is filtered and dried to remove impurities and excess water;

[0055] S6. Seal the prepared bacterial agent and store it at low temperature.

[0056] Example 3

[0057] The preparation method of the microbial agent for root nematodes comprises the following steps:

[0058] S1. Prepare the following materials: 600 parts of avermectin, 500 parts of purpurogenous fungi, 700 parts of long-branched trichoderma, 500 parts of potassium dihydrogen phosphate, 400 parts of carbonamide, and 350 parts of fish protein organic fertilizer;

[0059] S2, heating potassium dihydrogen phosphate, carbonamide, and fish protein organic fertilizer in a stirring tank and uniformly mixing;

[0060] S3. Adding avermectin, purpurogenous spores, and long-branched Trichoderma inoculants to the preliminarily mixed nutrients, and stirring thoroughly with a stirrer to ensure that the inoculants and nutrients are fully mixed without agglomeration or stratification;

[0061] S4, placing the mixture into a fermentation tank, sealing it and fermenting it;

[0062] S5. After the fermentation is completed, the bacterial agent is filtered and dried to remove impurities and excess water;

[0063] S6. Seal the prepared bacterial agent and store it at low temperature.

[0064] Table 1 below is a reference table of the killing effects of microbial agents prepared in different embodiments on root nematodes:

[0065] Group cucumber seedlings Tomato seedlings Example 1 excellent excellent Example 2 excellent excellent Example 3 excellent excellent

[0066] Table 1

[0067] It can be seen from the above table that the prepared microbial agent has excellent ability to kill root nematodes, reflecting that the parameter performance is relatively excellent.

[0068] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0069] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A microbial agent for root nematodes, characterized in that: The invention is composed of the following ingredients: 100-600 parts of abamectin, 200-500 parts of purpurogenous fungus, 300-700 parts of long-branched trichoderma, 150-500 parts of potassium dihydrogen phosphate, 80-400 parts of carbonamide and 100-350 parts of fish protein organic fertilizer.

2. The microbial agent for root nematodes according to claim 1, wherein Specifically, the invention is composed of the following ingredients: 400 parts of avermectin, 240 parts of purpurogenous fungi, 400 parts of long-branched trichoderma, 280 parts of potassium dihydrogen phosphate, 256 parts of carbonamide, and 200 parts of fish protein organic fertilizer.

3. The microbial agent for root nematodes according to claim 1, wherein: The invention discloses a method for preparing a avermectin-producing Streptomyces sp. by inoculating it into a culture medium for suspension culture. The temperature is required to be 27-29 DEG C and the culture time is 50-60 hours. A centrifuge is used to separate the bacterial body from the culture solution to obtain bacterial body powder. The bacterial body powder is mixed with ethanol and stirred at a stirring speed of 60 rpm for 1 hour. Impurities in the solution are removed by filtration to obtain an avermectin solution. A solvent crystallization method is used to obtain avermectin crystals. The solvent in the crystals is removed by drying to prepare avermectin.

4. The microbial agent for root nematodes according to claim 1, wherein: A PDA culture medium is prepared by adding water to a potato extract, glucose, and agar powder to a fixed volume, inoculating a strain of the purpurogenous fungus into the prepared culture medium under sterile conditions, placing the inoculated culture medium in a constant temperature incubator, and culturing at a temperature of 28°C and humidity conditions, regularly observing the growth of the strain, adjusting the culture conditions as needed, and performing a propagation operation to obtain a sufficient bacterial volume when the strain grows well on the culture medium. The propagated strain is extracted by a liquid fermentation method to obtain spore powder, and the purpurogenous fungus is obtained by freeze-drying.

5. The microbial agent for root nematodes according to claim 1, wherein: Peat, vermiculite, cow dung and mushroom residue are mixed, and purified water is used to dissolve the culture medium raw materials, and the solution is added to a fermentation tank. The water in the fermentation tank is heated to 50°C, and the long-branch Trichoderma species is inoculated into the fermentation tank. The preset temperature is 20°C-35°C, the pH value of the fermentation liquid is controlled at 5.5-6.5, and the fermentation time is 72h-120h. After the fermentation is completed, the Trichoderma is mixed with a wetting agent, a dispersant and a disintegrant, and then granulated and dried in a fluidized bed to form water-dispersible granules to prepare the long-branch Trichoderma.

6. The microbial agent for root nematodes according to claim 1, characterized in that: Potassium nitrate and phosphoric acid are mixed in a ratio of 2:1, water is added to the mixture to fully dissolve it, and the mixed solution is transferred to a reaction vessel, heated to 80°C-90°C and continuously stirred. After the reaction is completed, the solution is allowed to stand and cool, and potassium dihydrogen phosphate is separated using a centrifuge. The separated potassium dihydrogen phosphate is dried to form anhydrous crystals to produce potassium dihydrogen phosphate.

7. The microbial agent for root nematodes according to claim 1, characterized in that: The purified ammonia and carbon dioxide are mixed in a molar ratio of 2.8-4.5 and then fed into a synthesis tower. The pressure in the tower is 13-25 MPa, the temperature is 180°C-200°C, and the residence time of the reaction materials is 25min-40min to obtain a carbonamide solution containing excess ammonia and ammonium carbamate. The urea solution after the ammonia and ammonium carbamate are separated is evaporated to 99.5% by reducing pressure and cooling, and then granulated in a granulation tower to obtain carbonamide.

8. The microbial agent for root nematodes according to claim 1, characterized in that: After the discarded fish bones are dried, they are ground into powder using a grinder. The fish bone powder is placed in a fermentation barrel and a fermentation agent is added. The fish bone powder is fully stirred and mixed. The fermentation time is 168h-240h. After the fish bone powder is decomposed, it is dried in the sun. After grinding, fine fertilizer powder is obtained to produce fish protein organic fertilizer.

9. A method for preparing a microbial agent specifically for root nematodes, characterized in that: The preparation steps include: S1. Prepare the corresponding number of materials; S2, heating potassium dihydrogen phosphate, carbonamide, and fish protein organic fertilizer in a stirring tank and uniformly mixing; S3. Adding avermectin, purpurogenous spores, and long-branched Trichoderma inoculants to the preliminarily mixed nutrients, and stirring thoroughly with a stirrer to ensure that the inoculants and nutrients are fully mixed without agglomeration or stratification; S4, placing the mixture into a fermentation tank, sealing it and fermenting it; S5. After the fermentation is completed, the bacterial agent is filtered and dried to remove impurities and excess water; S6. Seal the prepared bacterial agent and store it at low temperature.

10. The method for preparing the special microbial agent for root nematodes according to claim 9, wherein: In step S3, the stirring speed is 80 rpm and the stirring time is 1.5 h. In step S4, the temperature of the fermentation tank is set at 25° C.-37° C. and the fermentation time is 72 h-120 h.

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