Preparation method of functional selenium-enriched drinking water for improving health of pets

Abstract

The invention discloses a preparation method of functional selenium-rich drinking water for improving pet health, and relates to the technical field of pet food, and the preparation method comprises the following steps: I, selecting an organic selenium source, designing a material formula, preparing small molecular selenium peptide based on the material formula, and preparing a selenium peptide aqueous solution by using the prepared small molecular selenium peptide; according to the selenium-rich water, the bioavailability of selenium can be remarkably improved, the potential toxicity risk caused by high-dose intake is avoided, pets can take in selenium element more efficiently so as to improve the immunity and the oxidation resistance, the health characteristic of the product is considered, the palatability is remarkably improved, the problems that traditional selenium-rich water is prone to precipitation and poor in stability are solved, and the selenium-rich water is suitable for being eaten by pets. Powerful support is provided for large-scale production and market promotion, and the practical value in the aspects of relieving stress and improving immunity is achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of pet food, and in particular to a method for preparing functional selenium-rich drinking water for improving the health of pets. Background Art

[0002] With the increasing prevalence of pet ownership, pet health issues are receiving increasing attention. However, due to their desert evolutionary history, cats generally suffer from a congenital lack of water intake, with their average daily water intake reaching only about 70% of their physiological needs. This insufficient water intake leads to concentrated and crystallized urine, increasing the risk of urinary tract diseases. Furthermore, pets are prone to decreased food intake and elevated cortisol levels (up to 40% or more) under stressful conditions (such as environmental changes or medical procedures), further weakening their immune system and posing a serious threat to their health.

[0003] To address this problem, existing solutions on the market have many limitations. For example, although wet food with high water content can alleviate the problem of insufficient water intake for pets to a certain extent, it is difficult to become a long-term effective solution due to its perishable nature and high protein content that may increase the burden on the kidneys. At the same time, the organic selenium used in ordinary health products or supplements has a large molecular weight (>5000Da), low bioavailability (only about 20%), and some products have excessive sodium content (>50mg / L), which increases the burden on the pet's urinary system. What is more noteworthy is that traditional selenium-enriched water is prone to selenium precipitation (Se 0 The problems of precipitation) and corruption and deterioration seriously affect the stability and safety of the product.

[0004] Despite its important physiological functions for animal health, traditional selenium-enriched products in pet food have been less than ideal. This is not only due to the low absorption efficiency of organic selenium, but also due to its difficulty synergizing with other nutrients to achieve optimal health benefits. Furthermore, existing preparation processes often fail to effectively address the stability issues of selenium-enriched water, resulting in inconsistent product quality. Therefore, we propose a method for preparing functional selenium-enriched drinking water to improve pet health. Summary of the Invention

[0005] The purpose of the present invention is to solve the defects in the prior art and provide a method for preparing functional selenium-rich drinking water that improves the health of pets.

[0006] The present invention proposes a method for preparing functional selenium-rich drinking water for improving pet health. The technical solution adopted to solve the technical problem is:

[0007] Ⅰ. Select an organic selenium source and design a material formula. Prepare small molecule selenopeptides based on the material formula. At the same time, use the prepared small molecule selenopeptides to prepare a selenopeptide aqueous solution;

[0008] II. The selenium peptide aqueous solution is subjected to dynamic high-pressure homogenization treatment, and then the homogenized selenium peptide aqueous solution is subjected to antiseptic treatment and aseptically canned to obtain the final selenium-enriched drinking water;

[0009] III. Through a four-channel drinking fountain comparative experiment, the quality of selenium-enriched drinking water was tested, and then high-performance liquid chromatography and dynamic light scattering technology were used to monitor and control the molecular weight of selenopeptides in real time.

[0010] As a further embodiment of the present invention, the material formula in step I specifically includes a Candidautilis cas15 yeast strain that has been bred through mutagenesis, sodium ions, and calcium and magnesium ions;

[0011] The highly active hydrolyzate of the Candida utilis cas15 yeast strain is an organic selenium source; the sodium ion content in selenium-enriched drinking water is controlled at ≤15 mg / L; and the calcium-magnesium ion ratio is Ca:Mg=2:1.

[0012] As a further solution of the present invention, the specific steps of preparing small molecule selenopeptides based on the material formula in step I are as follows:

[0013] S1.1: The yeast strain Candida utilis cas15 is cultured and treated by cell wall disruption and enzymatic hydrolysis to obtain a yeast hydrolyzate containing small molecule peptides and organic selenium. The yeast hydrolyzate is then removed from the enzymatic hydrolysis tank and coarsely filtered through an 80-100 mesh stainless steel sieve to initially remove large insoluble particles, bacterial fragments, and unreacted raw materials.

[0014] S1.2: Place the yeast hydrolyzate obtained by coarse filtration into a centrifuge at 8000 rpm for 15 minutes at 4-10°C. Filter the supernatant of the yeast hydrolyzate through a 0.45 μm pore size membrane filter to obtain a clarified yeast hydrolyzate. Place the clarified yeast hydrolyzate into a low-speed stirring system and slowly mix it at 60-100 rpm.

[0015] S1.3: The pretreated yeast hydrolysate is placed in a spray drying device for high-temperature atomization treatment, and the temperature of the spray drying device is controlled at 160-180°C. The protein in the yeast hydrolysate is cracked into small-molecule selenopeptides through spray drying. The small-molecule selenopeptides are then compared with traditional yeast selenium, and the results of the improved bioavailability of the prepared small-molecule selenopeptides are recorded.

[0016] As a further embodiment of the present invention, the specific steps of performing dynamic high-pressure homogenization on the selenopeptide aqueous solution in step II are as follows:

[0017] S2.1: Dissolve the small molecule selenopeptide powder obtained by spray drying in purified water or a low ion concentration base solution according to the formula ratio and pre-mix using a magnetic stirrer at a speed of 50-100 rpm. After mixing, use DLS to measure the particle size of the initial selenopeptide aqueous solution and record it;

[0018] S2.2: The prepared selenopeptide aqueous solution is slowly injected into the dynamic high-pressure homogenizer, the pressure is set to 30 MPa, and the homogenization treatment is carried out at room temperature. The selenopeptide aqueous solution passes through the microporous impact valve in the high-pressure homogenizer, and an instantaneous pressure drop, cavitation, shear and turbulence treatment occur, so that the particles in the mixed liquid are quickly split and dispersed. The homogenized selenopeptide aqueous solution is returned to the dynamic high-pressure homogenizer and repeatedly cycled through the dynamic high-pressure homogenization treatment twice;

[0019] S2.3: After three dynamic high-pressure homogenization treatments are completed, the DLS instrument is used again to detect the particle size distribution of the homogenized selenopeptide aqueous solution. When the test results show that the particle size of the selenopeptide aqueous solution has dropped to 85nm, and the distribution is narrow and there is no obvious agglomeration phenomenon, it indicates that the particle size range of the selenopeptide aqueous solution has reached the nanometer level. The selenopeptide aqueous solution that passes the test should be immediately placed in a sealed inert environment container for storage.

[0020] As a further embodiment of the present invention, the specific steps of preservative treatment of the homogenized selenopeptide aqueous solution and aseptic packaging in step II are as follows:

[0021] S3.1: Weigh zinc citrate as a buffer and a trace amount of preservative, then fully dissolve the zinc citrate in warm water. Slowly add the mixture to the homogenized selenopeptide aqueous solution while gently stirring. Then, use a pH meter to monitor the pH value of the selenopeptide aqueous solution in real time.

[0022] S3.2: If the pH is lower than 5.5, slowly add sodium hydroxide solution to increase the pH value. If the pH is higher than 6.0, add citric acid solution for fine-tuning until the pH value of the selenopeptide aqueous solution is stable between 5.5 and 6.0;

[0023] S3.3: After the buffering treatment is completed, the selenopeptide aqueous solution is sent to the high-temperature instantaneous sterilization system for rapid sterilization. After the sterilization is completed, the selenopeptide aqueous solution is immediately cooled, and then the sterilized selenopeptide aqueous solution is introduced into the aseptic filling system for canning, and is immediately heat-sealed or screw-capped after filling.

[0024] As a further solution of the present invention, the specific steps of testing the quality of selenium-enriched drinking water through the four-channel drinking fountain comparative experiment in step III are as follows:

[0025] S4.1: Select a pre-determined number of healthy adult cats or dogs and conduct a 7-day palatability test using a four-channel drinking fountain comparative test apparatus. Provide the selected experimental animals with selenium-enriched water, regular mineral water, distilled water, or blank water.

[0026] S4.2: Record the frequency of each animal's choice of different drinking channels and the amount of water it drinks daily. Videotape the behavior to analyze preference and calculate the proportion of preferred choices. If the selenium-enriched water selection rate is higher than that of other groups, it indicates that the product has good palatability.

[0027] S4.3: Experimental pets were randomly divided into an experimental group receiving selenium-enriched water and a control group receiving standard water, simulating typical stress environments such as transportation, starvation, and noise. Observation was conducted for 5-7 days. Changes in weight, food intake, and mental status of the animals were recorded before and after the experiment. Blood samples were collected from the experimental pets to measure their corresponding serum cortisol levels.

[0028] S4.4: Place the bottled selenium-enriched drinking water samples at room temperature in a light-proof environment, and set the storage period to 12 months. Take samples of the bottled selenium-enriched drinking water samples every 1 to 2 months, test the selenium precipitation rate, and record it.

[0029] Beneficial effects of the present invention:

[0030] The present invention treats Candida utilis cas15 yeast through cell wall breaking and enzymatic hydrolysis to prepare yeast hydrolyzate containing small molecule peptides and organic selenium. The clarified liquid is obtained by coarse filtration, centrifugation and membrane filtration, and then spray-dried to crack into small molecule selenium peptide powder. The powder is dissolved in purified water in proportion, pre-mixed by magnetic stirring and particle size detection, and then nano-processed three times using dynamic high-pressure homogenization technology to reduce the particle size to below 85nm. After homogenization, zinc citrate is added to adjust the pH to 5.5-6.0, followed by high-temperature instant sterilization and aseptic filling. The finished selenium-enriched drinking water has passed palatability experiments, anti-stress verification and long-term stability tests, and can significantly improve the bioavailability of selenium, avoid the potential toxicity risks brought by high-dose intake, and enable pets to more efficiently absorb selenium to enhance immunity and antioxidant capacity. It not only takes into account the health characteristics of the product, but also significantly improves palatability, solves the problems of easy precipitation and poor stability of traditional selenium-enriched water, and also provides strong support for large-scale production and market promotion. It has practical value in relieving stress and enhancing immunity. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] The present invention will be further described below with reference to the accompanying drawings.

[0032] Figure 1 This is a framework diagram of a method for preparing functional selenium-rich drinking water to improve pet health. DETAILED DESCRIPTION

[0033] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0034] Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative work shall fall within the scope of protection of the present invention.

[0035] The present invention provides a method for preparing functional selenium-rich drinking water that improves the health of pets. Figure 1 , Figure 1 This is a framework diagram of a method for preparing functional selenium-rich drinking water that improves pet health, provided by an embodiment of the present invention. The system includes the following steps:

[0036] Select an organic selenium source and design a material formula, produce small molecule selenopeptides based on the material formula, and use the produced small molecule selenopeptides to prepare a selenopeptide aqueous solution.

[0037] Specifically, the yeast strain Candida utilis cas15 is cultured by cell wall breaking and enzymatic hydrolysis, and a yeast hydrolyzate containing small molecule peptides and organic selenium is obtained. The yeast hydrolyzate is then drawn out of the enzymatic hydrolysis tank and coarsely filtered through an 80-100 mesh stainless steel sieve to preliminarily remove large insoluble particles, bacterial fragments and unreacted raw materials. The yeast hydrolyzate obtained by coarse filtration is placed in a centrifuge, and the centrifuge speed is set to 8000 rpm. The yeast hydrolyzate is centrifuged for 15 minutes at a temperature of 4-10°C, and the yeast hydrolyzate after centrifugation is filtered through a membrane filter with a pore size of 0.45 μm. The supernatant of the liquid was filtered twice to obtain the clarified liquid of the yeast hydrolyzate, and then the filtered clarified liquid was placed in a low-speed stirring system and slowly mixed at a speed of 60-100 rpm. The pretreated yeast hydrolyzate was placed in a spray drying equipment for high-temperature atomization treatment, and the temperature of the spray drying equipment was controlled at 160-180°C. The protein in the yeast hydrolyzate was cracked into small-molecule selenopeptides through spray drying. The small-molecule selenopeptides were then compared with traditional yeast selenium, and the results of the improved bioavailability of the prepared small-molecule selenopeptides were recorded.

[0038] It should be further explained that the material formula specifically includes the Candi da utiliscas15 yeast strain that has been bred through mutagenesis, sodium ions, and calcium and magnesium ions;

[0039] Among them, the Candida utilis cas15 yeast strain is cultured under specific conditions to ensure that the organic selenium content in its hydrolysate is ≥500μg / g, and it also contains rich nutrients, and is used as a highly active hydrolysate as a source of organic selenium; the sodium ion content in selenium-enriched drinking water is controlled at ≤15mg / L; and the calcium and magnesium ion ratio is Ca:Mg=2:1.

[0040] The selenium peptide aqueous solution is subjected to dynamic high-pressure homogenization treatment, and then the homogenized selenium peptide aqueous solution is subjected to antiseptic treatment and aseptically canned to obtain the final selenium-enriched drinking water.

[0041] In addition, it should be noted that when the temperature of the spray drying equipment reaches 180°C, more than 95% of the protein is broken down into small peptides with a molecular weight of less than 1000 Da.

[0042] Specifically, the small molecule selenopeptide powder obtained by spray drying is dissolved in purified water or low ion concentration base liquid according to the formula ratio, and pre-mixed at a speed of 50-100 rpm using a magnetic stirrer. After the mixing is completed, the particle size of the initial selenopeptide aqueous solution is detected by DLS and recorded. The prepared selenopeptide aqueous solution is slowly injected into a dynamic high-pressure homogenizer with a set pressure of 30 MPa. The homogenization treatment is carried out at room temperature. The selenopeptide aqueous solution passes through a microporous impact valve in the high-pressure homogenizer, and instantaneous pressure drop, cavitation, shear and turbulence occur. The particles in the mixed liquid are quickly split and dispersed, and the homogenized selenopeptide aqueous solution is returned to the dynamic high-pressure homogenizer, and then repeatedly cycled through the dynamic high-pressure homogenization treatment twice. After the three dynamic high-pressure homogenization treatments are completed, the particle size distribution of the homogenized selenopeptide aqueous solution is detected again using the DLS instrument. When the test results show that the particle size in the selenopeptide aqueous solution has dropped to 85nm, and the distribution is narrow and there is no obvious agglomeration phenomenon, it indicates that the particle size range of the selenopeptide aqueous solution has reached the nanometer level, and the selenopeptide aqueous solution that has passed the test is immediately placed in a sealed inert environment container for storage.

[0043] Specifically, zinc citrate is weighed as a buffer and a trace amount of preservatives, and then zinc citrate is fully dissolved in warm water. The mixture is slowly added to the homogenized selenopeptide aqueous solution while gently stirring. Then, the pH value change of the selenopeptide aqueous solution is monitored in real time using a pH meter. If the pH is lower than 5.5, sodium hydroxide solution is slowly added dropwise to increase the pH value. If the pH is higher than 6.0, citric acid solution is added for fine-tuning until the pH value of the selenopeptide aqueous solution is stabilized between 5.5 and 6.0. After the buffering treatment is completed, the selenopeptide aqueous solution is sent to a high-temperature instantaneous sterilization system for rapid sterilization. After the sterilization is completed, the selenopeptide aqueous solution is immediately cooled, and then the sterilized selenopeptide aqueous solution is introduced into a sterile filling system for canning, and is immediately heat-sealed or screw-capped after filling.

[0044] It should be further explained that the particle size of the particles in the selenopeptide aqueous solution was reduced from 1200nm to 85nm through three dynamic high-pressure homogenization treatments, ensuring the uniformity and stability of the product.

[0045] Through a four-channel drinking fountain comparison experiment, the quality of selenium-rich drinking water was tested, and then high-performance liquid chromatography and dynamic light scattering technology were used to monitor and control the molecular weight of selenopeptides in real time.

[0046] Specifically, a preset number of healthy adult cats or dogs are selected, and a 7-day palatability test cycle is conducted. A four-channel drinking fountain comparison experimental device is used to provide the selected experimental animals with selenium-rich water, ordinary mineral water, distilled water and blank water groups respectively. The frequency of each animal's choice of different drinking channels and the amount of water it drinks are recorded every day, and the behavior is videotaped to analyze the choice preference, and the preference selection ratio is calculated. If the selenium-rich water selection rate is higher than that of other groups, it indicates that the product has excellent palatability. Typical stress environments such as transportation, hunger and noise are simulated, and the experimental pets are randomly divided into an experimental group drinking selenium-rich water and a control group drinking ordinary water. The animals are observed for 5 to 7 days, and the weight changes, food intake and mental state of the animals before and after the experiment are recorded. Blood samples of the experimental pets are collected to detect the corresponding serum cortisol concentration. The bottled selenium-rich drinking water samples are placed at room temperature in a light-proof environment, and the storage period is set to 12 months. The bottled selenium-rich drinking water samples are sampled every 1 to 2 months, and the selenium precipitation rate is detected and recorded.

[0047] It should be noted that the palatability test results show that the proportion of pets choosing selenium-rich water reached 70%, which is more than 65% higher than the traditional water in the control group. When monitored under stress conditions, selenium-rich drinking water can reduce the weight loss rate of pets from the traditional 5% to <2%, and reduce serum cortisol concentration by 40%, significantly improving the ability to resist stress. Using long-term room temperature storage tests, the test results showed that the selenium precipitation rate was as low as 0.1%, far lower than the more than 15% of traditional processes.

[0048] The above is a detailed description of an embodiment of the present invention. However, the content described is only a preferred embodiment of the present invention and should not be considered to limit the scope of the present invention. All equivalent changes and improvements made within the scope of the present invention should still fall within the scope of the patent coverage of the present invention.

Claims

1. A method for preparing functional selenium-rich drinking water for improving pet health, characterized in that: The following steps are involved: Ⅰ. Select an organic selenium source and design a material formula. Prepare small molecule selenopeptides based on the material formula. At the same time, use the prepared small molecule selenopeptides to prepare a selenopeptide aqueous solution; II. The selenium peptide aqueous solution is subjected to dynamic high-pressure homogenization treatment, and then the homogenized selenium peptide aqueous solution is subjected to antiseptic treatment and aseptic canning to obtain the final selenium-enriched drinking water; III. Through a four-channel drinking fountain comparative experiment, the quality of selenium-enriched drinking water was tested, and then high-performance liquid chromatography and dynamic light scattering technology were used to monitor and control the molecular weight of selenopeptides in real time.

2. The method for preparing functional selenium-rich drinking water for improving pet health according to claim 1, characterized in that: The material formula of step I specifically includes the Candi da utilis cas15 yeast strain that has been bred through mutagenesis, sodium ions, and calcium and magnesium ions; The highly active hydrolyzate of the Candida utilis cas15 yeast strain is an organic selenium source; the sodium ion content in selenium-enriched drinking water is controlled at ≤15 mg / L; and the calcium-magnesium ion ratio is Ca:Mg=2:

1.

3. The method for preparing functional selenium-rich drinking water for improving pet health according to claim 2, wherein: The specific steps for preparing small molecule selenopeptides based on the material formula described in step I are as follows: S1.1: The yeast strain Candida utilis cas15 is cultured and treated by cell wall disruption and enzymatic hydrolysis to obtain a yeast hydrolyzate containing small molecule peptides and organic selenium. The yeast hydrolyzate is then removed from the enzymatic hydrolysis tank and coarsely filtered through an 80-100 mesh stainless steel sieve to initially remove large insoluble particles, bacterial fragments, and unreacted raw materials. S1.2: Place the yeast hydrolyzate obtained by coarse filtration into a centrifuge at 8000 rpm for 15 minutes at 4-10°C. Filter the supernatant of the yeast hydrolyzate through a 0.45 μm pore size membrane filter to obtain a clarified yeast hydrolyzate. Place the clarified yeast hydrolyzate into a low-speed stirring system and slowly mix it at 60-100 rpm. S1.3: The pretreated yeast hydrolysate is placed in a spray drying device for high-temperature atomization treatment, and the temperature of the spray drying device is controlled at 160-180°C. The protein in the yeast hydrolysate is cracked into small-molecule selenopeptides through spray drying. The small-molecule selenopeptides are then compared with traditional yeast selenium, and the results of the improved bioavailability of the prepared small-molecule selenopeptides are recorded.

4. The method for preparing functional selenium-rich drinking water for improving pet health according to claim 3, characterized in that: The specific steps of the dynamic high-pressure homogenization treatment of the selenopeptide aqueous solution in step II are as follows: S2.1: Dissolve the small molecule selenopeptide powder obtained by spray drying in purified water or a low ion concentration base solution according to the formula ratio and pre-mix using a magnetic stirrer at a speed of 50-100 rpm. After mixing, use DLS to measure the particle size of the initial selenopeptide aqueous solution and record it; S2.2: The prepared selenopeptide aqueous solution is slowly injected into the dynamic high-pressure homogenizer, the pressure is set to 30 MPa, and the homogenization treatment is carried out at room temperature. The selenopeptide aqueous solution passes through the microporous impact valve in the high-pressure homogenizer, and an instantaneous pressure drop, cavitation, shear and turbulence treatment occur, so that the particles in the mixed liquid are quickly split and dispersed. The homogenized selenopeptide aqueous solution is returned to the dynamic high-pressure homogenizer and repeatedly cycled through the dynamic high-pressure homogenization treatment twice; S2.3: After three dynamic high-pressure homogenization treatments are completed, the DLS instrument is used again to detect the particle size distribution of the homogenized selenopeptide aqueous solution. When the test results show that the particle size of the selenopeptide aqueous solution has dropped to 85nm, and the distribution is narrow and there is no obvious agglomeration phenomenon, it indicates that the particle size range of the selenopeptide aqueous solution has reached the nanometer level. The selenopeptide aqueous solution that passes the test should be immediately placed in a sealed inert environment container for storage.

5. The method for preparing functional selenium-rich drinking water for improving pet health according to claim 4, characterized in that: The specific steps of preservative treatment of the homogenized selenopeptide aqueous solution and aseptic filling in step II are as follows: S3.1: Weigh zinc citrate as a buffer and a trace amount of preservative, then fully dissolve the zinc citrate in warm water. Slowly add the mixture to the homogenized selenopeptide aqueous solution while gently stirring. Then, use a pH meter to monitor the pH value of the selenopeptide aqueous solution in real time. S3.2: If the pH is lower than 5.5, slowly add sodium hydroxide solution to increase the pH value. If the pH is higher than 6.0, add citric acid solution for fine-tuning until the pH value of the selenopeptide aqueous solution is stable between 5.5 and 6.0; S3.3: After the buffering treatment is completed, the selenopeptide aqueous solution is sent to the high-temperature instantaneous sterilization system for rapid sterilization. After the sterilization is completed, the selenopeptide aqueous solution is immediately cooled, and then the sterilized selenopeptide aqueous solution is introduced into the aseptic filling system for canning, and is immediately heat-sealed or screw-capped after filling.

6. The method for preparing functional selenium-rich drinking water for improving pet health according to claim 5, characterized in that: The specific steps for testing the quality of selenium-enriched drinking water through the four-channel drinking fountain comparison experiment described in step III are as follows: S4.1: Select a pre-determined number of healthy adult cats or dogs and conduct a 7-day palatability test using a four-channel drinking fountain comparative test apparatus. Provide the selected experimental animals with selenium-enriched water, regular mineral water, distilled water, or blank water. S4.2: Record the frequency of each animal's choice of different drinking channels and the amount of water it drinks daily. Videotape the behavior to analyze preference and calculate the proportion of preferred choices. If the selenium-enriched water selection rate is higher than that of other groups, it indicates that the product has good palatability. S4.3: Experimental pets were randomly divided into an experimental group receiving selenium-enriched water and a control group receiving standard water, simulating typical stress environments such as transportation, starvation, and noise. Observation was conducted for 5-7 days. Changes in weight, food intake, and mental status of the animals were recorded before and after the experiment. Blood samples were collected from the experimental pets to measure their corresponding serum cortisol levels. S4.4: Place the bottled selenium-enriched drinking water samples at room temperature in a light-proof environment, and set the storage period to 12 months. Take samples of the bottled selenium-enriched drinking water samples every 1 to 2 months, test the selenium precipitation rate, and record it.

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