Skin conditioning composition containing gamma-polyglutamic acid, application and product

Abstract

The invention belongs to the technical field of health-care food, and relates to a gamma-polyglutamic acid-containing composition for skin conditioning, application and a product. The composition for skin conditioning is prepared from the following components in parts by weight: 1 to 50 parts of gamma-polyglutamic acid, 5 to 20 parts of yeast beta-glucan, 0.1 to 0.5 part of non-denatured type II collagen and 0.1 to 2 parts of plant extract, the plant extract is a grape seed extract and / or a Chinese waxgourd seed extract. The components in the composition are reasonably compounded, and the composition has the dual effects of improving skin moisture and removing freckles. Meanwhile, the components are simple and safe and can be taken for a long time.

Description

Technical Field

[0001] The present invention belongs to the technical field of health-care foods and relates to a skin conditioning composition containing gamma-polyglutamic acid, and its application and product. Background Art

[0002] The epidermis is the part of the skin that comes into direct contact with the outside world. The stratum corneum, stratum lucidum, and stratum granulosum are key to retaining moisture. The stratum corneum, in particular, regulates water evaporation and absorption, and protects against the invasion of external substances such as chemicals, toxic substances, and bacteria, acting as a skin barrier.

[0003] To hydrate and lighten skin spots, cosmetics with corresponding benefits can be used. Ingredients like hyaluronic acid, glycerin, and ceramides can directly penetrate the stratum corneum to quickly relieve dryness. Ingredients like arbutin, vitamin C, and niacinamide can inhibit melanin production and nitrogenize surface pigmentation. Topical cosmetics offer immediate results, such as hydrating masks and serums, which can quickly boost skin hydration. They also enable targeted, targeted care, such as using spot-lightening products on blemishes to reduce irritation to surrounding skin. However, these products require long-term use and cannot fundamentally address dehydration and pigmentation. Taking foods with corresponding benefits internally can effectively address this shortcoming.

[0004] Internal supplements can fundamentally improve skin condition by regulating the body's immune and metabolic functions, achieving the effects of improving skin hydration and lightening dark spots. For example, Chinese invention patent application CN104489700A discloses a composition and its use for improving skin hydration, elasticity, and wrinkle reduction. The active ingredients of the composition are primarily collagen peptides and elastin peptides. The composition also includes the following active components: sodium hyaluronate and traditional Chinese medicine extracts. These active ingredients work together to improve skin hydration, elasticity, and wrinkle reduction through multiple pathways and levels. The active ingredients, the composite peptide, the traditional Chinese medicine composition, and the sodium hyaluronate in the composition synergistically improve skin hydration, elasticity, and wrinkle reduction.

[0005] For example, Chinese invention patent application CN116326783A discloses a composition with skin moisture improvement and anti-oxidation functions and its preparation method. The composition includes the following raw materials in parts by weight: 2550 parts of fish collagen tripeptide; 1040 parts of marine fish oligopeptide; 9-32 parts of sodium hyaluronate; 3-24 parts of tea polysaccharide; 1-15 parts of bonito elastin peptide; 0.02-10 parts of Haematococcus pluvialis; and 1-5 parts of artichoke extract. The composition uses small molecule collagen and low molecular weight peptides, which are more conducive to being fully absorbed by the human body, forming a fine elastic network in the skin, reducing skin wrinkles, firmly locking in moisture and supporting the skin, and achieving a beauty and moisturizing effect.

[0006] γ-Polyglutamic acid (γ-PGA) is a naturally occurring anionic polypeptide that is water-soluble, biodegradable, and highly biocompatible, making it widely used in food and pharmaceutical applications. γ-PGA also has high water absorption, moisture retention, and moisturizing properties, making it a common humectant in cosmetics. However, in the food industry, it is primarily used as a food additive to improve flavor and texture. However, the water absorption and moisturizing properties of γ-PGA in food have been less studied. Summary of the Invention

[0007] The present invention addresses the problems of the prior art and provides a skin conditioning composition containing γ-polyglutamic acid, its application, and product. The skin conditioning composition comprises γ-polyglutamic acid, non-denatured type II collagen, yeast β-glucan, and plant extracts; the plant extracts include grape seed extract and / or wax gourd seed extract. The components of the composition work synergistically to significantly improve skin hydration and reduce dark spots.

[0008] One of the purposes of the present invention is:

[0009] Provided is a composition for skin conditioning, comprising: 1-50 parts of gamma-polyglutamic acid, 5-20 parts of yeast beta-glucan, 0.1-0.5 parts of non-denatured type II collagen, and 0.1-2 parts of a plant extract; the plant extract is grape seed extract and / or wax gourd seed extract.

[0010] Preferably, the skin conditioning composition comprises, by weight, 10-30 parts of γ-polyglutamic acid, 8-15 parts of yeast β-glucan, 0.1-0.3 parts of non-denatured type II collagen, and 0.8-1.2 parts of plant extract.

[0011] In some preferred embodiments of the present invention, the skin conditioning composition comprises, by weight, 20 parts of γ-polyglutamic acid, 10 parts of yeast β-glucan, 0.2 parts of non-denatured type II collagen, and 1 part of plant extract.

[0012] In other preferred embodiments of the present invention, the skin conditioning composition comprises, by weight, 30 parts of γ-polyglutamic acid, 15 parts of yeast β-glucan, 0.15 parts of non-denatured type II collagen, and 0.2 parts of plant extract.

[0013] γ-Polyglutamic acid (γ-PGA), also known as natto gum, is a viscous high-molecular-weight biopolymer. It is a glutamic acid polymer formed by the dehydration condensation of the α-amino group of D-glutamic acid and / or L-glutamic acid with the γ-carboxyl group of the adjacent glutamic acid. It is highly hydrophilic and absorbs water well.

[0014] Non-denatured type II collagen refers to type II collagen that retains its intact macromolecular triple helix structure after extraction. Studies have shown that it can effectively reduce inflammation and pain in the treatment of rheumatoid arthritis and osteoarthritis. Furthermore, studies have demonstrated broad safety and good cell compatibility, promoting cell proliferation, and using various toxicology tests, including acute oral toxicity, acute skin toxicity, primary skin irritation, and primary eye irritation, in rabbits. This makes it a valuable raw material for pharmaceuticals and health foods.

[0015] Yeast beta-glucan is a natural dietary fiber found in yeast cell walls. It can bind to specific receptors and enter the lymphatic system through endocytosis, thereby exerting an immunomodulatory effect. Research has shown that beta-glucan can not only regulate intestinal flora, repair intestinal mucosa, enhance immunity, promote bowel movements, lower blood sugar and blood lipids, relieve fatigue, and repair liver damage, but also has significant biological activity. It can enter the skin and activate Langerhans cells, promoting the production of cytokines, growth factors, and angiogenic factors, thereby achieving skin repair, regeneration, and wrinkle reduction.

[0016] Grape seed extract (GSE) is a natural product extracted from the seeds of grape berries, typically red or green grapes. Its main active ingredients include proanthocyanidins (OPCs), polyphenols, vitamin E, and linoleic acid, all of which have potent antioxidant properties. Grape seed extract has a wide range of applications, particularly in antioxidant, anti-aging, cardiovascular health, skin care, anti-inflammatory, and immune regulation.

[0017] The main active ingredients of Benincasa hispida Seed Extract include saponins, fats, urea, citrulline, and urease. It has the effects of clearing heat, resolving phlegm, and discharging pus and dampness. It also regulates and improves microcirculation, maintaining skin moisture. In cosmetics, it provides excellent moisturizing and oil-control properties.

[0018] More preferably, the mass ratio of the grape seed extract to the wax gourd seed extract is 1:3-6.

[0019] More preferably, the molecular weight Mn of the γ-polyglutamic acid is 100-300 kDa.

[0020] A second object of the present invention is to provide use of any of the above-mentioned compositions in the preparation of skin conditioning products.

[0021] Furthermore, the skin conditioning product is a product having the effects of improving skin moisture and / or removing spots.

[0022] Furthermore, the skin conditioning product is a health food; the dosage form of the health food includes any one of a solution, a syrup, a powder, a granule, a tablet, a capsule, a pill, a cream, a plaster and a decoction.

[0023] The third purpose of the present invention is:

[0024] Provided is a health food comprising any one of the above-mentioned skin conditioning compositions and food-acceptable excipients.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] The skin conditioning composition of the present invention has a simple composition and rationally formulated components. Yeast β-glucan regulates the body's immune function and promotes skin repair; non-denatured type II collagen promotes cell proliferation; and the water-retaining properties of γ-polyglutamic acid prevent skin moisture loss. The three components work synergistically to form a more complete skin barrier. Wax gourd seed extract improves microcirculation, providing oxygen and nutrients to the skin's keratinocytes and fibroblasts while removing skin metabolites to reduce dark spots. Grape seed extract has antioxidant properties, and when combined with wax gourd seed extract, its lightening effect is enhanced to a certain extent, achieving an overall improvement in skin moisture and lightening. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the embodiments described are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. It is worth noting that the raw materials used in the present invention are all common commercially available products, and their sources are not specifically limited.

[0028] Example

[0029] This embodiment provides a skin conditioning composition containing γ-polyglutamic acid. The components are taken according to the composition and amount in Table 1, and stirred and mixed evenly to obtain the composition.

[0030] Table 1 Composition and dosage of each composition (parts by weight)

[0031]

[0032]

[0033] Application Examples

[0034] Preparation of oral liquid for skin conditioning: Add the above compositions into water to a concentration of 1 g / L, heat to 45°C and stir for 5 minutes, then homogenize, sterilize and fill to obtain oral liquid 1-12 (specification: 100 mL / bottle).

[0035] Human food test 1

[0036] 130 subjects (all female) aged 30-50 years with skin moisture ≤12 were selected and randomly divided into 13 groups (there were no significant differences in age and skin moisture among the subjects in each group). The experimental groups 1-12 took the above-mentioned oral liquids 1-12 respectively. The dosage was: once in the morning and once in the evening every day, 1 bottle each time, for 2 consecutive months. The control group served as a blank control. During this period, the subjects in each group did not use other cosmetics or foods with corresponding functional effects.

[0037] Skin moisture was measured before, after one month, and after two months of use. Testing method: While the subjects were resting, they cleaned the test area with a clean cotton ball dipped in distilled water. After drying, the moisture content of the skin between the forehead and eyebrows was measured 15 minutes later. Moisture was measured using the CM825 skin moisture test probe from German company CK. The measurements were performed using the same instrument and by the same person. The test results are shown in Table 2.

[0038] Statistical analysis: The results were SPSS10.0 statistical software was used for statistical analysis, and t-test was used. Paired t-test was used for self-control, and group t-test was used for comparison of means between two groups. The latter required a variance homogeneity test. Appropriate variable transformation was performed on non-normally distributed or heterogeneous variance data. After the normal variance was met, the transformed data was used for testing. If the transformed data still could not meet the normal variance requirement, t' test was used instead. However, if the coefficient of variation was too large (such as CV>50%), rank sum test was applied.

[0039] Table 2 Skin moisture content test results

[0040]

[0041] Note: When comparing data in the same row, the same letters do not indicate significant differences; when comparing data in the same column, the same values do not indicate significant differences.

[0042] As can be seen from the above table, compared with the control group, the skin moisture content of the subjects in experimental groups 1-6 and experimental groups 11 and 12 was significantly improved, but compared with before taking, the experimental groups 1-6 showed significant improvement after taking for one month, indicating that the compositions 1-6 of the present invention are significantly effective in improving skin moisture.

[0043] Human food test 2

[0044] 1. Experimental subjects and groups:

[0045] 130 patients (all female) aged 30-50 years with melasma were selected as subjects and randomly divided into 13 groups according to the color and area of chloasma (there were no significant differences in age, chloasma color, and area among the subjects in each group). The experimental groups 1-12 took the above-mentioned oral liquids 1-12, respectively, once in the morning and evening every day, 1 bottle each time; the control group took oral vitamins (vitamin C 200 mg + vitamin E 100 mg), once in the morning and evening every day; all took the medicines for 2 consecutive months, during which time the subjects in each group did not use other cosmetics or foods with corresponding functional effects.

[0046] Inclusion criteria for the subjects: light brown to dark brown patches on the face with clear boundaries, usually symmetrically distributed, without inflammatory manifestations or scaling; no obvious subjective symptoms; mainly occurring after puberty, more common in women; the condition has a certain seasonality, being more severe in summer and milder in winter; no obvious endocrine disease, and pigmentation caused by other diseases is excluded.

[0047] Exclusion criteria for subjects: those under 18 years old or over 65 years old, pregnant or breastfeeding women, those with allergic constitutions and those who are allergic to this product; those with serious diseases such as cardiovascular, cerebrovascular, liver, kidney and hematopoietic system and endocrine diseases, and mental patients; alcoholics or smokers; those who take items related to the test function in the short term, which affects the judgment of the results; those who do not take the test sample as required, and the efficacy cannot be determined or the incomplete data affects the judgment of efficacy or safety.

[0048] 2. Test methods and result determination:

[0049] Testing method: The size and color depth of the facial melasma of the subjects were tested before taking the product and after taking it for 2 months. The results are shown in Table 3 and Table 4 respectively. The size of facial melasma was measured with a ruler (mm). 2 ); the brown (Y+M+BK, a combination of yellow, magenta, and black) color chart in the "Standard Color Chart for Use" (first edition) designed and developed by the Institute of Geography, Chinese Academy of Sciences and published by the China Press for Surveying and Mapping in 1992 is used as the standard for judging the depth of melasma: degree I (15, 20, 5), degree II (30, 40, 10), and degree III (40, 60, 15).

[0050] Statistical analysis: The results were SPSS 10.0 statistical software was used for statistical analysis, paired t test was used for self-comparison, group t test was used for inter-group comparison, and x test was used for efficiency. 2 test.

[0051] Table 3 Comparison of color changes of chloasma

[0052]

[0053]

[0054] Note: When comparing data in the same row, the same letters do not indicate significant differences; when comparing data in the same column, the same values do not indicate significant differences.

[0055] Table 4 Comparison of changes in chloasma area

[0056]

[0057] Note: When comparing data in the same row, the same letters do not indicate significant differences; when comparing data in the same column, the same values do not indicate significant differences.

[0058] The results in Tables 3 and 4 show that compared with the control group, the color and area of chloasma in experimental groups 1-6 decreased significantly, indicating that the composition of the present invention has a significant freckle-lightening effect. In addition, compared with experimental group 1, the percentage of chloasma color reduction in experimental groups 5 and 6 was smaller, indicating that the simultaneous use of grape seed extract and wax gourd seed extract, within a specific ratio range, can further enhance the freckle-lightening effect of the composition.

[0059] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A skin conditioning composition containing γ-polyglutamic acid, characterized in that The composition comprises: 1-50 parts of gamma-polyglutamic acid, 5-20 parts of yeast beta-glucan, 0.1-0.5 parts of non-denatured type II collagen and 0.1-2 parts of plant extract; the plant extract is grape seed extract and / or wax gourd seed extract.

2. The skin conditioning composition according to claim 1, wherein The composition comprises, by weight, 10-30 parts of gamma-polyglutamic acid, 8-15 parts of yeast beta-glucan, 0.1-0.3 parts of non-denatured type II collagen and 0.8-1.2 parts of plant extract.

3. The skin conditioning composition according to claim 2, wherein The composition comprises, by weight, 20 parts of gamma-polyglutamic acid, 10 parts of yeast beta-glucan, 0.2 parts of non-denatured type II collagen, and 1 part of plant extract.

4. The skin conditioning composition according to claim 2, wherein The composition comprises, by weight, 10 parts of gamma-polyglutamic acid, 15 parts of yeast beta-glucan, 0.15 parts of non-denatured type II collagen, and 0.8 parts of plant extract.

5. The skin conditioning composition according to any one of claims 1 to 4, characterized in that The mass ratio of the grape seed extract and wax gourd seed extract is 1:3-6.

6. The skin conditioning composition according to any one of claims 1 to 4, characterized in that The molecular weight Mn of the gamma-polyglutamic acid is 100-300 kDa.

7. Use of the skin conditioning composition according to any one of claims 1 to 6 in the preparation of skin conditioning products.

8. The use according to claim 7, characterized in that The skin conditioning product is a product that has the effects of improving skin moisture and / or removing spots.

9. The use according to claim 8, characterized in that The product is a health food; the dosage form includes any one of solution, syrup, powder, granule, tablet, capsule, pill, cream, plaster and decoction.

10. A health food, characterized in that: The skin conditioning composition comprises the skin conditioning composition according to any one of claims 1 to 5 and a food-acceptable auxiliary material.

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