Whitening and anti-saccharification white tricholoma matsutake freeze-dried powder and preparation method thereof

Abstract

The invention discloses whitening and anti-saccharification tricholoma matsutake freeze-dried powder and a preparation method thereof, and relates to the technical field of skin care product preparation. The freeze-dried powder is prepared from 20 to 30 parts of type I collagen, 15 to 25 parts of white tricholoma matsutake extracting solution, 5 to 10 parts of lucid ganoderma extract, 3 to 8 parts of algal polysaccharide extract, 0.5 to 2 parts of astaxanthin, 1 to 3 parts of vitamin E, 0.1 to 0.5 part of alkannin, 10 to 15 parts of mannitol and 2 to 5 parts of sodium hyaluronate. All the components have a synergistic effect and jointly endow the freeze-dried powder with anti-saccharification and whitening functions.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care product preparation, and more particularly to a whitening and anti-glycation white pine mushroom freeze-dried powder and a preparation method thereof. Background Art

[0002] Skin whitening and anti-glycation are two core demands in the modern skincare industry. Excessive melanin deposition can lead to dull skin tone and dark spots, while glycation accelerates skin aging, forming advanced glycation end products (AGEs), which cause loss of elasticity and wrinkles. Although a variety of whitening and anti-glycation products are currently available on the market, most offer limited whitening effectiveness. Traditional whitening products (such as those containing arbutin and niacinamide) primarily inhibit tyrosinase activity but have limited effect on improving glycation damage. Anti-glycation products (such as those containing carnosine and lipoic acid) can inhibit AGE formation, but their whitening effectiveness is insufficient. Many whitening ingredients are susceptible to oxidation and inactivation, while anti-glycation ingredients are susceptible to thermal degradation, leading to decreased efficacy during storage or use. Large molecular weight active ingredients have difficulty penetrating the skin barrier, resulting in insufficient bioavailability and impacting the ultimate effect. Traditional serums and lotions are susceptible to microbial contamination, and some photosensitive ingredients are less stable in liquid systems. Conventional lyophilized powders often suffer from poor resolubility or loss of activity due to formulation or processing issues. Many freeze-dried powders are susceptible to moisture, oxidation or degradation of active ingredients during storage, resulting in reduced efficacy, especially photosensitive and heat-sensitive ingredients that are difficult to maintain activity for a long time.

[0003] Therefore, there is an urgent need to develop a freeze-dried powder with high stability, good absorbability, and dual effects of whitening and anti-glycation, and to solve the above problems by optimizing the formula and process. Summary of the Invention

[0004] In order to achieve the above-mentioned object, the present invention provides a whitening and anti-glycation white pine mushroom freeze-dried powder and a preparation method thereof.

[0005] The first aspect of the present invention provides a whitening and anti-glycation freeze-dried powder of Agaricus thunbergii, which is composed of the following components in parts by weight:

[0006] 20-30 parts of type I collagen, 15-25 parts of white matsutake extract, 5-10 parts of ganoderma lucidum extract, 3-8 parts of seaweed polysaccharide extract, 0.5-2 parts of astaxanthin, 1-3 parts of vitamin E, 0.1-0.5 parts of shikonin, 10-15 parts of mannitol, and 2-5 parts of sodium hyaluronate.

[0007] The functions of each component are as follows:

[0008] White pine mushroom extract: contains pine mushroom polysaccharides, pine mushroom polypeptides and tyrosinase inhibitors, which can inhibit the activity of melanocytes, block the oxidation of dopaquinone into melanin, and at the same time scavenge free radicals and reduce ultraviolet-induced melanin deposition.

[0009] Ganoderma lucidum extract contains polysaccharides that can enhance the skin's antioxidant capacity and reduce the damage of AGEs (advanced glycation end products) to collagen.

[0010] Astaxanthin has an antioxidant capacity 6,000 times greater than vitamin C. It can quench singlet oxygen, scavenge free radicals, inhibit UV-induced skin inflammation and melanin production, and block the non-enzymatic glycation reaction between sugar and protein. It forms an antioxidant network with vitamin E, enhancing whitening and anti-aging effects.

[0011] Shikonin inhibits tyrosinase activity, reduces melanin synthesis, and has anti-inflammatory effects, improving pigmentation caused by inflammation.

[0012] Type I collagen directly replenishes collagen in the dermis, reducing cross-linking damage to pre-existing collagen caused by AGEs and restoring skin elasticity. It also acts as a carrier, promoting the penetration of other ingredients. Its molecular weight is 2000-3000 Da, ensuring water solubility and absorption.

[0013] The polysaccharide structure in seaweed polysaccharide extract is similar to that of skin hyaluronic acid, which can competitively bind to sugar molecules and reduce protein glycation; at the same time, it forms a moisturizing film, strengthens the skin barrier, and reduces glycation reactions caused by external stimuli.

[0014] Vitamin E scavenges free radicals, protects cell membranes from oxidative damage, and combines with astaxanthin and shikonin to enhance the whitening effect while inhibiting skin aging caused by lipid peroxidation.

[0015] Sodium hyaluronate locks in moisture, maintains skin hydration, promotes the penetration of collagen, matsutake extract and other ingredients into the dermis, and enhances anti-glycation and whitening efficiency.

[0016] Mannitol is used as an excipient to prevent protein (collagen) denaturation of the freeze-dried powder during the freeze-drying process, while regulating the osmotic pressure to ensure product stability.

[0017] In addition to their individual effects, the above components also have synergistic effects. For example, the polysaccharides in the white matsutake extract form a hydrogen bond network with the phenolic hydroxyl groups of shikonin, which wraps the astaxanthin oil droplets during the freeze-drying process and reduces its oxidative polymerization (the astaxanthin retention rate after freeze-drying reaches 91%, an increase of 23% compared with freeze-drying alone); the three together with mannitol form a porous ice crystal structure, which quickly releases the active ingredients when re-dissolved to ensure transdermal absorption efficiency.

[0018] The quinone structure of shikonin acts as an oxidation buffer for astaxanthin. When astaxanthin is oxidized, shikonin restores its activity through electron transfer, forming an "antioxidant regeneration system." The polyphenols in the white pine mushroom extract work synergistically with shikonin to chelate metal ions, preventing metal ion-catalyzed oxidation of astaxanthin's double bonds and improving the formula's stability. Therefore, the synergistic effect of the white pine mushroom extract, shikonin, and astaxanthin imparts whitening and anti-glycation properties to the freeze-dried powder.

[0019] Furthermore, there is a synergistic effect between Ganoderma lucidum extract and type I collagen. Through molecular structural complementarity, physical interactions, and cellular signaling coordination, type I collagen and Ganoderma lucidum extract form a three-dimensional protective system against glycation. The triple helical structure of type I collagen, composed of α1 and α2 chains, is rich in free amino groups and RGD sequences. The triterpenes in Ganoderma lucidum extract contain phenolic hydroxyl groups, while the polysaccharide possesses a β-glucan triple helical conformation. The interaction of these two creates a multi-dimensional synergistic effect. At the molecular level, the free amino groups at the termini of type I collagen peptide chains actively undergo a Maillard reaction with glucose, acting as a "decoy" to reduce glycation of other proteins. The phenolic hydroxyl groups of Ganoderma lucidum triterpenes, with a pKa value of 7.2, undergo nucleophilic substitution with glycation intermediates. Simultaneously, they form hydrogen bonds with the cysteine sulfhydryl group in the active site of aldose reductase with a binding energy of -8.7 kcal / mol, inhibiting enzyme activity and reducing the concentration of glycated substrates at the source. The combined effects enhance the clearance of AGE precursors. From the perspective of physical space, the nanofiber network self-assembled by type I collagen and the triple-helix hydrogel formed by Ganoderma lucidum polysaccharide β-glucan construct an interpenetrating network through the hydrogen bonding between the hydroxyl and carbonyl groups, which enables the slow release of the free amino groups of type I collagen and prolongs the anti-glycemic effect.

[0020] The second aspect of the present invention provides a method for preparing the whitening and anti-glycation white pine mushroom freeze-dried powder, comprising the following steps:

[0021] (1) Mixing and dissolving: Prepare a white matsutake extract, dissolve collagen I, sodium hyaluronate, and mannitol in pure water, heat to 35-45° C., and stir until completely dissolved to obtain an aqueous phase;

[0022] (2) Emulsification and dispersion: Add astaxanthin and vitamin E to the white pine mushroom extract, homogenize and emulsify, and then mix with water to obtain an emulsion;

[0023] (3) Low-temperature addition: cool the emulsion to 15-25°C, add Ganoderma lucidum extract, seaweed polysaccharide extract, and shikonin, and stir in the dark for 30-40 minutes to obtain a mixed solution;

[0024] (4) Pre-freezing and freeze-drying: freeze-dry the mixture to obtain freeze-dried powder.

[0025] Compared with the existing technology, type I collagen, sodium hyaluronate, and mannitol are weighed according to the formula weight and dissolved together in pure water. The temperature is heated to 35-45°C. This temperature range can accelerate dissolution and avoid collagen denaturation caused by excessively high temperature. At the same time, stirring is performed, and the stirring speed is controlled at 150-200rpm until all ingredients are completely dissolved and a uniform aqueous phase is obtained. In this process, mannitol can be used as a freeze-drying protectant to prevent denaturation of other ingredients during subsequent freeze-drying. After the sodium hyaluronate and collagen are dissolved, they can lay a stable foundation for subsequent mixing.

[0026] Astaxanthin and vitamin E are added to the prepared white pine mushroom extract. Since astaxanthin and vitamin E are fat-soluble, a homogenized emulsification process is employed to ensure their uniform dispersion, forming tiny particles that are evenly dispersed throughout the extract. The emulsified solution is then mixed with the aqueous phase obtained in the first step, with continuous stirring, to obtain a uniform and stable emulsion.

[0027] Cool the emulsion to 15-25°C. This low temperature minimizes the loss of activity in heat-sensitive ingredients such as Ganoderma lucidum extract, seaweed polysaccharide extract, and shikonin. Add the three ingredients to the emulsion in the dark, ensuring thorough mixing to obtain a mixed solution. This darkening procedure prevents oxidation and deterioration of ingredients like shikonin in the presence of light.

[0028] The mixed solution is divided into freeze-drying containers and placed in a pre-freezing chamber to completely freeze the mixed solution into a solid state. Subsequently, the pre-frozen sample is transferred to a freeze-drying chamber for freeze-drying to obtain whitening and anti-glycation white pine mushroom freeze-dried powder.

[0029] Furthermore, the preparation method of the white matsutake extract is: after the white matsutake fruiting body is crushed, an ethanol solution with a mass concentration of 60% is added at a weight-to-volume ratio of 1:10 to 1:15, ultrasonic extraction is carried out at 50-60°C for 2 hours, and then filtered and concentrated under reduced pressure to a solid content of not less than 30%.

[0030] Furthermore, the reduced pressure concentration is 0.06-0.09 MPa concentration for 1-2 hours.

[0031] Furthermore, in step (1), the total mass of collagen I, sodium hyaluronate, and mannitol to pure water is adjusted to a mass volume ratio of 1 g: 100-200 mL, and the stirring is performed at 300-400 rpm for 5-10 minutes.

[0032] Furthermore, in step (2), the speed of homogenization is 500-600 rpm, and the time is 20-30 min.

[0033] Furthermore, in step (3), the stirring speed is 200-300 rpm, and the stirring time is 10-15 min.

[0034] Furthermore, in step (4), the freeze drying is performed by pre-freezing at -40 to -50°C for 3-4 hours, then freezing at -25 to -30°C for 1-2 hours with a vacuum degree of 10 Pa; and finally sublimation drying at 20 to 25°C for 10 to 12 hours.

[0035] The third aspect of the present invention provides the use of the freeze-dried powder in the field of whitening and skin care.

[0036] Compared with the prior art, when the stirring speed is adopted, the components are ensured to be mixed evenly so as to obtain a uniform and stable system. DETAILED DESCRIPTION

[0037] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0038] Unless otherwise stated in the following examples, all raw materials are commercially available.

[0039] Example 1

[0040] A whitening and anti-glycation freeze-dried powder of Agaricus thunbergii, comprising the following components in parts by weight:

[0041] 20 parts of type I collagen, 25 parts of white pine mushroom extract, 5 parts of ganoderma lucidum extract, 3 parts of seaweed polysaccharide extract, 0.5 parts of astaxanthin, 1 part of vitamin E, 0.1 parts of shikonin, 10 parts of mannitol, and 2 parts of sodium hyaluronate.

[0042] The preparation method of the above-mentioned freeze-dried powder comprises the following steps:

[0043] (1) Mixed dissolution: Prepare a white matsutake extract, and dissolve I collagen, sodium hyaluronate, and mannitol in pure water, heat to 35°C and stir until completely dissolved to obtain an aqueous phase; wherein the mass volume ratio of the total mass of I collagen, sodium hyaluronate, and mannitol to pure water is 1 g:100 mL; stir at 300 rpm for 5 min.

[0044] (2) Emulsification and dispersion: Astaxanthin and vitamin E were added to the white pine mushroom extract, homogenized and emulsified, and then mixed with water to obtain an emulsion; the speed of homogenization was 500 rpm and the time was 20 min;

[0045] (3) Low-temperature addition: cool the emulsion to 15°C, add Ganoderma lucidum extract, seaweed polysaccharide extract, and shikonin, and stir in the dark for 30 minutes to obtain a mixed solution; the stirring speed is 200 rpm and the stirring time is 10 minutes.

[0046] (4) Pre-freezing and freeze-drying: The mixture is freeze-dried to obtain freeze-dried powder. Freeze-drying is performed by pre-freezing at -40°C for 3 hours, then freezing at -25°C for 1 hour with a vacuum of 10 Pa; and finally sublimation drying at 20°C for 10 hours.

[0047] The preparation method of the white matsutake extract comprises the following steps: crushing the white matsutake fruiting bodies, adding a 60% ethanol solution at a weight-to-volume ratio of 1:10, ultrasonically extracting at 50° C. for 2 hours, filtering, and concentrating at 0.06 MPa for 1 hour, and then concentrating under reduced pressure until the solid content is not less than 30%.

[0048] Example 2

[0049] A whitening and anti-glycation freeze-dried powder of Agaricus thunbergii, comprising the following components in parts by weight:

[0050] 30 parts of type I collagen, 15 parts of white matsutake extract, 10 parts of ganoderma lucidum extract, 8 parts of seaweed polysaccharide extract, 2 parts of astaxanthin, 3 parts of vitamin E, 0.5 parts of shikonin, 15 parts of mannitol, and 5 parts of sodium hyaluronate.

[0051] The preparation method of the above-mentioned freeze-dried powder comprises the following steps:

[0052] (1) Mixed dissolution: Prepare a white matsutake extract, and dissolve I collagen, sodium hyaluronate, and mannitol in pure water, heat to 45°C and stir until completely dissolved to obtain an aqueous phase; wherein the mass volume ratio of the total mass of I collagen, sodium hyaluronate, and mannitol to pure water is 1 g:200 mL; stir at 400 rpm for 10 min.

[0053] (2) Emulsification and dispersion: Astaxanthin and vitamin E were added to the white pine mushroom extract, homogenized and emulsified, and then mixed with water to obtain an emulsion; the speed of homogenization was 600 rpm and the time was 30 min;

[0054] (3) Low-temperature addition: cool the emulsion to 25°C, add Ganoderma lucidum extract, seaweed polysaccharide extract, and shikonin, and stir in the dark for 40 minutes to obtain a mixed solution; the stirring speed is 300 rpm and the stirring time is 15 minutes.

[0055] (4) Prefreezing and freeze drying: freeze-dry the mixture to obtain freeze-dried powder. Freeze drying is to prefreeze at -50℃ for 4 hours, then freeze at -30℃ for 2 hours, with a vacuum degree of 10Pa; and finally sublimate and dry at 25℃ for 12 hours.

[0056] The preparation method of the white matsutake extract comprises the following steps: crushing the white matsutake fruiting bodies, adding a 60% ethanol solution at a weight-to-volume ratio of 1:15, ultrasonically extracting at 60° C. for 2 hours, filtering, and concentrating at 0.09 MPa for 2 hours, and then concentrating under reduced pressure until the solid content is not less than 30%.

[0057] Example 3

[0058] A whitening and anti-glycation freeze-dried powder of Agaricus thunbergii, comprising the following components in parts by weight:

[0059] 25 parts of type I collagen, 20 parts of white pine mushroom extract, 7 parts of ganoderma lucidum extract, 5 parts of seaweed polysaccharide extract, 1 part of astaxanthin, 2 parts of vitamin E, 0.3 parts of shikonin, 12 parts of mannitol, and 3 parts of sodium hyaluronate.

[0060] The preparation method of the above-mentioned freeze-dried powder comprises the following steps:

[0061] (1) Mixed dissolution: Prepare a white matsutake extract, and dissolve type I collagen, sodium hyaluronate, and mannitol in pure water, heat to 40°C, and stir until completely dissolved to obtain an aqueous phase; wherein the mass volume ratio of the total mass of type I collagen, sodium hyaluronate, and mannitol to pure water is 1 g:150 mL; stir at 350 rpm for 8 min.

[0062] (2) Emulsification and dispersion: Astaxanthin and vitamin E were added to the white pine mushroom extract, homogenized and emulsified, and then mixed with water to obtain an emulsion; the speed of homogenization was 550 rpm and the time was 25 min;

[0063] (3) Low-temperature addition: cool the emulsion to 20°C, add Ganoderma lucidum extract, seaweed polysaccharide extract, and shikonin, and stir in the dark for 35 minutes to obtain a mixed solution; the stirring speed is 230 rpm and the stirring time is 13 minutes.

[0064] (4) Prefreezing and freeze drying: The mixture is freeze-dried to obtain freeze-dried powder. Freeze drying is performed by prefreezing at -40 to -50°C for 3.5 hours, then freezing at -30°C for 1.5 hours with a vacuum of 10 Pa; and finally sublimation drying at 25°C for 11 hours.

[0065] The preparation method of the white matsutake extract comprises the following steps: crushing the white matsutake fruiting bodies, adding a 60% ethanol solution at a weight-to-volume ratio of 1:12, ultrasonically extracting at 55° C. for 2 hours, filtering, and concentrating at 0.07 MPa for 1.5 hours. The extraction is then concentrated under reduced pressure until the solid content is no less than 30%.

[0066] Comparative Example 1

[0067] Taking Example 3 as an example, compared with Example 3, the white matsutake extract was removed and the weight portion of astaxanthin was increased to 21 parts; the other components and methods remained unchanged.

[0068] Comparative Example 2

[0069] Taking Example 3 as an example, compared with Example 3, astaxanthin was removed and the weight portion of the white matsutake extract was increased to 21 parts; the other components and methods remained unchanged.

[0070] Comparative Example 3

[0071] Taking Example 3 as an example, compared with Example 3, shikonin was removed and the weight portion of astaxanthin was increased to 1.3 parts; the other components and methods remained unchanged.

[0072] Comparative Example 4

[0073] Taking Example 3 as an example, compared with Example 3, type I collagen was removed and the weight portion of the Ganoderma lucidum extract was increased to 32 parts; the other components and methods remained unchanged.

[0074] Comparative Example 5

[0075] Taking Example 3 as an example, compared with Example 3, the Ganoderma lucidum extract was removed and the weight portion of type I collagen was increased to 32 parts; the other components and methods remained unchanged.

[0076] Tyrosinase inhibition rate determination (whitening effect)

[0077] Method: L-DOPA oxidation method was used

[0078] The samples from Examples 1-3 and Comparative Examples 1-5 were dissolved in PBS (pH 6.8), mixed with tyrosinase (100 U / mL) and L-DOPA (0.5 mM), and reacted at 37°C for 30 min. The absorbance at 475 nm was measured, and the inhibition rate was calculated using arbutin (1 mg / mL) as a positive control:

[0079] Inhibition rate (%) = (1-A sample / A blank) × 100

[0080] Melanin content determination (B16F10 cell model)

[0081] method:

[0082] Recovery and Passaging: B16F10 melanoma cells were removed from liquid nitrogen and rapidly thawed in a 37°C water bath. The cell suspension was transferred to DMEM medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin and cultured in a 37°C, 5% CO2 incubator. When the cells reached 80%-90% confluence, they were digested and passaged using 0.25% trypsin-EDTA. Cells in the logarithmic growth phase were selected for subsequent experiments.

[0083] Cell seeding: B16F10 cells were plated at 5×10 4The cells were seeded at a density of 100 μL in a 96-well cell culture plate, 100 μL of culture medium was added to each well, and the plates were cultured in an incubator for 24 h to allow the cells to adhere to the wall and grow.

[0084] Freeze-dried powder processing

[0085] Sample preparation: The lyophilized powders of Examples 1 to 3 and Comparative Examples 1 to 5 were weighed separately and prepared into a stock solution with a concentration of 10 mg / mL using sterile PBS buffer. After sterilization by filtration through a 0.22 μm filter membrane, the stock solution was diluted with culture medium into 5 concentration gradients (e.g., 100, 50, 25, 12.5, and 6.25 μg / mL).

[0086] Grouping and processing:

[0087] Experimental group: 100 μL of lyophilized powder solution with different concentration gradients was added to each well, and 6 replicate wells were set for each concentration;

[0088] Control group: 100 μL of culture medium was added to each well of the blank control group;

[0089] For the positive control group (e.g., kojic acid group), 100 μL of culture medium containing 20 μg / mL kojic acid was added to each well. The culture plate was placed in an incubator and cultured for 48 hours.

[0090] Cell lysis and melanin determination

[0091] Cell lysis: Carefully aspirate the culture medium in the 96-well plate, add 100 μL of cell lysis solution (PBS containing 1% TritonX-100) to each well, and place on ice for 30 minutes. During this period, gently shake the culture plate to ensure complete cell lysis.

[0092] Melanin extraction: Place a 96-well plate at 4°C and centrifuge at 12,000 rpm for 10 min. Transfer 50 μL of the supernatant to a new 96-well plate. Add 50 μL of 1 mol / L NaOH solution to each well and incubate in an 80°C water bath for 1 h to fully dissolve the melanin.

[0093] Absorbance determination: After the sample is cooled to room temperature, use a microplate reader to measure the absorbance (OD value) at 405 nm.

[0094] Reduction index (%) = (1-melanin content of sample group / melanin content of blank group) × 100

[0095] Skin brightness (L* value) and moisture content (Corneometer method)

[0096] Skin brightness test:

[0097] Eighty volunteers (female, aged 25-45 years) were recruited and divided into eight groups of ten participants each. Each group was treated with an emulsion containing 5% of the lyophilized powders of Examples 1-3 and Comparative Examples 1-5 twice daily for 28 days. Lightness (L value) of the inner forearm was measured using a colorimeter (CIE L*a*b), and skin moisture content was measured using a Corneometer CM825.

[0098] Table 1: In vitro efficacy evaluation results

[0099]

[0100]

[0101] *Positive control: 2% arbutin (whitening), 10% glycerin (moisturizing).

[0102] Table 2: Human skin test results (28 days)

[0103]

[0104]

[0105] As can be seen from Tables 1 and 2, Example 3 has the best overall performance (inhibition rate of 80.5%, melanin reduction of 45.3%), indicating the synergistic effect of the white pine mushroom extract with astaxanthin and shikonin. The whitening effect of Comparative Example 1 (without white pine mushroom) was significantly reduced, confirming the key role of the white pine mushroom extract. The skin moisture content of Example 3 increased by 30.1%, thanks to the synergistic moisturizing effect of sodium hyaluronate and seaweed polysaccharides. Comparative Example 4 (without collagen) had the worst moisturizing performance, suggesting the importance of type I collagen in maintaining skin hydration.

[0106] Component synergy:

[0107] The whitening effect of Comparative Example 3 (no shikonin) was reduced, indicating that despite the low dosage, shikonin contributes to tyrosinase inhibition. The formula of Example 3 (20 parts of white pine mushroom extract + 1 part of astaxanthin + 0.3 parts of shikonin) performed optimally in terms of whitening, anti-glycation, and moisturizing, demonstrating that each component achieves synergistic effects through different mechanisms (tyrosinase inhibition, anti-oxidation, and moisturizing). These comparative results further validate the irreplaceable role of the core ingredients.

[0108] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0109] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A whitening and anti-glycation freeze-dried powder of Agaricus thunbergii, characterized in that: It is composed of the following components in parts by weight: 20-30 parts of type I collagen, 15-25 parts of white matsutake extract, 5-10 parts of ganoderma lucidum extract, 3-8 parts of seaweed polysaccharide extract, 0.5-2 parts of astaxanthin, 1-3 parts of vitamin E, 0.1-0.5 parts of shikonin, 10-15 parts of mannitol, and 2-5 parts of sodium hyaluronate.

2. The method for preparing the whitening and anti-glycation white pine thrush freeze-dried powder according to claim 1, which is used to prepare the freeze-dried powder according to claim 1, characterized in that: The following steps are involved: (1) Mixing and dissolving: Prepare a white matsutake extract, dissolve collagen I, sodium hyaluronate, and mannitol in pure water, heat to 35-45°C, and stir until completely dissolved to obtain an aqueous phase; (2) Emulsification and dispersion: Add astaxanthin and vitamin E to the white pine mushroom extract, homogenize and emulsify, and then mix with water to obtain an emulsion; (3) Low-temperature addition: cool the emulsion to 15-25°C, add Ganoderma lucidum extract, seaweed polysaccharide extract, and shikonin, and stir in the dark for 30-40 minutes to obtain a mixed solution; (4) Pre-freezing and freeze-drying: freeze-dry the mixture to obtain freeze-dried powder.

3. The method for preparing the whitening and anti-glycation white pine thrush freeze-dried powder according to claim 2, wherein: The preparation method of the white matsutake extract comprises the following steps: crushing the white matsutake fruiting bodies, adding a 60% ethanol solution at a weight-to-volume ratio of 1:10 to 1:15, ultrasonically extracting at 50-60° C. for 2 hours, filtering, and concentrating under reduced pressure until the solid content is not less than 30%.

4. The method for preparing the whitening and anti-glycation white pine thrush freeze-dried powder according to claim 3, wherein: The reduced pressure concentration is 0.06-0.09 MPa concentration for 1-2 hours.

5. The method for preparing the whitening and anti-glycation white pine thrush freeze-dried powder according to claim 2, characterized in that: In step (1), the total mass of collagen I, sodium hyaluronate, and mannitol to pure water is adjusted to a mass volume ratio of 1 g: 10-200 mL; and / or, The stirring is performed at 300-400 rpm for 5-10 min.

6. The method for preparing the whitening and anti-glycation white pine thrush freeze-dried powder according to claim 2, characterized in that: In step (2), the speed of homogenization is 500-600 rpm and the time is 20-30 min.

7. The method for preparing the whitening and anti-glycation freeze-dried powder of Agaricus thunbergii according to claim 2, characterized in that: In step (3), the rotation speed during stirring is 200-300 rpm, and the stirring time is 10-15 min.

8. The method for preparing the whitening and anti-glycation freeze-dried powder of Agaricus thunbergii according to claim 2, wherein: In step (4), the freeze drying is performed by pre-freezing at -40 to -50°C for 3 to 4 hours, then freezing at -25 to -30°C for 1 to 2 hours with a vacuum degree of 10 Pa; and finally sublimation drying at 20 to 25°C for 10 to 12 hours.

9. Use of the freeze-dried powder according to claim 1 in the field of whitening and skin care.

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