Skin care composition based on mixed chlorella extract as well as preparation method and application of skin care composition

Abstract

The invention provides a skin care composition based on a mixed chlorella extract as well as a preparation method and application of the skin care composition, and belongs to the technical field of cosmetics. The chlorella essence is prepared from the following raw material components in percentage by weight: 7%-15% of a mixed chlorella extract, 4%-10% of a synergist, 12%-30% of a humectant, 2.0%-8.0% of an emulsifier, 0.3%-0.6% of a thickening agent, 0.5%-1.2% of a preservative, 0.01%-0.4% of perfume and the balance of deionized water, the mixed chlorella extract is obtained by extracting chlorella pyrenoidosa, chlorella vulgaris and chlorella ellipsosa as raw materials. The chlorella pyrenoidosa, the chlorella vulgaris and the chlorella ellipsosa are used as raw materials to prepare the mixed chlorella extract, multiple effects and synergistic effects of the mixed chlorella extract are fully exerted, and the skin moisturizing and repairing effects are remarkably improved.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a skin care composition based on mixed chlorella extracts, a preparation method and an application thereof. Background Art

[0002] With the improvement of people's living standards and the pursuit of beauty, people are paying more and more attention to skin care issues. As an important microbial resource, Chlorella has a wide range of applications, such as in food, feed, cosmetics, and medicine. Chlorella is an algae rich in moisturizing ingredients. Chlorella extract is rich in various nutrients such as protein, amino acids, polysaccharides, and multiple vitamins. Most of these ingredients are hygroscopic and can help maintain moisture in the skin. Several authorized invention patents have proposed various methods, such as: CN115487100B for a cosmetic composition and cosmetics with oil-control and pore-refining effects, and CN118319799B for a skin care composition containing a microscopic Chlorella extract, its preparation method, and its application.

[0003] However, most of the current chlorella extracts are extracts of a single species of chlorella. The chemical composition of a single species of chlorella extract is relatively simple, and its functions and application range are relatively limited. Mixed chlorella extracts can play a role in more fields due to the diversity of their ingredients and the synergy of their functions. The ingredient control of mixed chlorella extracts is relatively complex, and the ratio of different types of chlorella and the extraction conditions need to be precisely controlled to ensure the stability of the extract's ingredients and functional consistency. Existing methods for preparing chlorella extracts usually involve complex extraction processes, such as acid hydrolysis, enzymatic hydrolysis, etc. These methods are not only cumbersome to operate, but also costly, which limits the widespread application of chlorella extracts in cosmetics. Therefore, it is of great significance to further study the ingredients and functions of mixed chlorella extracts and develop products with excellent skin care performance. Summary of the Invention

[0004] In view of the technical problems existing in the background technology, the present invention provides a skin care composition based on mixed chlorella extracts, a preparation method and application thereof, aiming to solve the technical problem that the chemical composition of a single type of chlorella extract in existing skin care products is relatively single and the skin care effect is limited.

[0005] In a first aspect, the present invention provides a skin care composition based on mixed chlorella extract, comprising the following raw material components by weight: 7% to 15% of mixed chlorella extract, 4% to 10% of a synergist, 12% to 30% of a moisturizer, 2.0% to 8.0% of an emulsifier, 0.3% to 0.6% of a thickener, 0.5% to 1.2% of a preservative, 0.01% to 0.4% of a fragrance, and the balance deionized water; Mixed chlorella extract is extracted from Chlorella pyrenoidosa, Chlorella vulgaris and Chlorella ellipsoidea as raw materials.

[0006] Preferably, the mass ratio of Chlorella pyrenoidosa, Chlorella vulgaris and Chlorella ellipsoidea is 1:(0.9-1.1):(0.9-1.1).

[0007] Preferably, the mass ratio of Chlorella pyrenoidosa, Chlorella vulgaris and Chlorella ellipsoidea is 1:1:1.

[0008] Preferably, the synergist includes at least one of vitamin C, vitamin E, astaxanthin, and asiaticoside.

[0009] Preferably, the moisturizing agent includes at least one of sodium hyaluronate, ceramide, butylene glycol, and 1,2-hexanediol.

[0010] Preferably, the emulsifier includes at least one of sucrose laurate, behenic acid, cetearyl alcohol, cholesterol, and polyglyceryl-10 stearate.

[0011] Preferably, the thickener includes at least one of carbomer, xanthan gum, polyacrylate crosspolymer-6, and guar gum.

[0012] Preferably, the preservative is selected from a mixture of two or more of potassium sorbate, sodium benzoate, ethylhexylglycerin and caprylyl glycol.

[0013] Preferably, the fragrance includes at least one of lavender essential oil, chamomile essential oil, tea tree essential oil, benzoate fragrance, and ethyl maltol.

[0014] In a second aspect, the present invention provides a method for preparing a skin care composition based on a mixed chlorella extract, comprising the following steps: The chlorella pyrenoidosa, chlorella vulgaris and chlorella ellipsoidea are mixed, dried and crushed, the crushed chlorella is mixed with ethanol, extracted by ultrasonic-assisted extraction, and centrifuged to obtain a chlorella extract; The chlorella extract is mixed with a synergist, a moisturizer, an emulsifier, a thickener, a preservative, a fragrance and deionized water, and then stirred evenly to obtain a skin care composition based on the mixed chlorella extract.

[0015] Preferably, the drying method includes microwave drying. The drying methods of the present invention include but are not limited to the microwave drying listed in the embodiments; researchers in this field can also choose other drying methods to achieve the drying effect according to actual conditions.

[0016] Preferably, the drying temperature is 50-70° C., and the drying time is 2-4 hours.

[0017] Preferably, the material-liquid ratio of the crushed Chlorella to ethanol is 1 g: (10-20) mL.

[0018] Preferably, the conditions of the ultrasonic-assisted extraction method are as follows: power 200-800W, time 5-30 minutes.

[0019] In a third aspect, the present invention provides a use of a skin care composition based on mixed Chlorella extracts in the preparation of cosmetics.

[0020] Preferably, the cosmetic comprises a toner, a lotion, a skin care composition, a face cream or a face mask.

[0021] The principle of the technical solution of the present invention is as follows: The polysaccharides in Chlorella pyrenoidosa and the unsaturated fatty acids in Chlorella vulgaris work together: the polysaccharides absorb moisture from the environment, while the fatty acids form a protective film on the skin's surface to prevent evaporation. This dual effect significantly enhances the moisturizing effect of the skin care composition, keeping the skin hydrated. The carotenoids and antioxidant enzymes in Chlorella ellipsoidea work synergistically with antioxidants such as vitamins C and E in Chlorella pyrenoidosa. Carotenoids directly absorb free radicals, while antioxidant enzymes catalyze their decomposition. Vitamins C and E regenerate the active forms of antioxidant enzymes, forming a highly effective antioxidant network that effectively scavenges free radicals from the skin and slows down skin aging. The combination of the three types of Chlorella provides a more comprehensive nutritional profile in the skin care composition, encompassing a variety of skin-beneficial ingredients, including proteins, polysaccharides, vitamins, unsaturated fatty acids, minerals, carotenoids, and more. This balanced nutrition meets the skin's diverse needs, ensuring that the skin is fully nourished while simultaneously performing its physiological functions, thereby comprehensively enhancing the efficacy of the skin care composition.

[0022] Compared with the prior art, the present invention has the following beneficial effects: (1) The present invention uses Chlorella pyrenoidosa, Chlorella vulgaris and Chlorella ellipsoidea as raw materials to prepare a mixed Chlorella extract, which gives full play to the multiple functions and synergistic effects of the mixed Chlorella extract. It combines a synergist, a moisturizer, an emulsifier, a thickener, a preservative, a fragrance and deionized water to prepare a skin care composition. The skin care composition significantly improves the skin moisturizing and repairing effects and has broad application prospects and market potential.

[0023] (2) Chlorella extract is rich in moisturizing ingredients that can help the skin retain moisture, improve dryness, roughness and other problems of the skin, and enhance the moisturizing effect of the skin care composition. Chlorella is rich in growth factor (CGF) that can promote the proliferation and differentiation of skin cells, enhance the skin's self-repair ability, enable the skin care composition to repair damaged cell tissues, and enhance the regeneration ability of normal cells, thereby improving skin problems.

[0024] (3) The ultrasonic-assisted extraction process was used to effectively improve the extraction efficiency and purity of Chlorella extract, and retain the integrity and biological activity of its active ingredients. DETAILED DESCRIPTION

[0025] The following embodiments of the technical solution of the present invention are described in detail. The following embodiments are only used to more clearly illustrate the technical solution of the present invention and are therefore only used as examples and are not intended to limit the scope of protection of the present invention.

[0026] For experimental methods in the examples where specific conditions are not specified, generally conventional conditions and conditions described in the manual or conditions recommended by the manufacturer were followed. The general equipment, materials, reagents, etc. used were all commercially available unless otherwise specified.

[0027] Chlorella pyrenoidosa was purchased from the Freshwater Algae Seed Bank of the National Aquatic Organism Germplasm Resources Bank (No.: FACHB-27).

[0028] Chlorella vulgaris was purchased from the Freshwater Algae Seed Bank of the National Aquatic Organisms Germplasm Resources Bank (No.: FACHB-2724).

[0029] Chlorella ellipsoidea was purchased from the Freshwater Algae Seed Bank of the National Aquatic Organism Germplasm Resources Bank (No.: FACHB-42).

[0030] 1. Preparation method Example 1 A method for preparing a skin care composition based on mixed chlorella extracts, comprising the following steps: S1. Preparation of composite chlorella: Chlorella pyrenoidosa, Chlorella vulgaris, and Chlorella ellipsoidea are mixed in a mass ratio of 1:1:1, and microwave-dried at 60°C for 2 hours; the dried chlorella is placed in a grinding homogenizer for crushing, and the crushed particles have a size of 100 mesh; the crushed chlorella is mixed with ethanol in a ratio of 1 g:10 mL, and extracted using an ultrasonic-assisted extraction method at a power of 300 W for 20 minutes; after the extraction is completed, the extract is centrifuged to remove impurities in the extract to obtain a chlorella extract.

[0031] S2. According to the formula shown in Table 1, a mixed chlorella extract, a synergist, a moisturizer, an emulsifier, a thickener, a preservative, a fragrance, and deionized water were mixed and stirred uniformly at a stirring speed of 150 r / min for 10 minutes. The mixture was then heated to 60° C. and stirred at a stirring speed of 250 r / min for 10 minutes to obtain a skin care composition based on a mixed chlorella extract.

[0032] Table 1

[0033] Example 2 A method for preparing a skin care composition based on mixed chlorella extracts, comprising the following steps: S1. Preparation of composite chlorella: Chlorella pyrenoidosa, Chlorella vulgaris, and Chlorella ellipsoidea are mixed in a mass ratio of 1:1:1, and microwave-dried at 70°C for 3 hours; the dried chlorella is placed in a grinding homogenizer for crushing, and the crushed particles have a size of 100 mesh; the crushed chlorella is mixed with ethanol in a ratio of 1 g:20 mL, and extracted using an ultrasonic-assisted extraction method at a power of 800 W for 5 minutes; after the extraction is completed, the extract is centrifuged to remove impurities in the extract to obtain a chlorella extract.

[0034] S2. According to the formula shown in Table 1, a mixed chlorella extract, a synergist, a moisturizer, an emulsifier, a thickener, a preservative, a fragrance, and deionized water were mixed and stirred uniformly at a stirring speed of 150 r / min for 10 minutes. The mixture was then heated to 60° C. and stirred at a stirring speed of 250 r / min for 10 minutes to obtain a skin care composition based on a mixed chlorella extract.

[0035] Example 3 A method for preparing a skin care composition based on mixed chlorella extracts, comprising the following steps: S1, the same as step S1 in Example 1; S2. According to the formula shown in Table 2, a mixed chlorella extract, a synergist, a moisturizer, an emulsifier, a thickener, a preservative, a fragrance, and deionized water were mixed and stirred uniformly at a stirring speed of 100 r / min for 15 minutes. The mixture was then heated to 50° C. and stirred at a stirring speed of 100 r / min for 15 minutes to obtain a skin care composition based on a mixed chlorella extract.

[0036] Table 2

[0037] Comparative Example 1 The difference between this comparative example and Example 1 is that, in step S1, Chlorella pyrenoidosa, Chlorella vulgaris, and Chlorella ellipsoidea are mixed at a mass ratio of 2:1:1, and the other steps are the same as those in Example 1.

[0038] Comparative Example 2 The difference between this comparative example and Example 1 is that in step S1, only Chlorella vulgaris is used, and the other steps are the same as those in Example 1.

[0039] Comparative Example 3 The difference between this comparative example and Example 1 is that in step S1, Chlorella pyrenoidosa is used alone, and the other steps are the same as those in Example 1.

[0040] Comparative Example 4 The difference between this comparative example and Example 1 is that in step S1, only Chlorella ellipsoidea is used, and the other steps are the same as those in Example 1.

[0041] Comparative Example 5 The difference between this comparative example and Example 1 is that in step S1, Chlorella pyrenoidosa is replaced by Spirulina, and the other steps are the same as those in Example 1.

[0042] 2. Test Method 1. Extract yield test: Weigh the mass of the chlorella raw material before extraction (m1), complete the extraction of the chlorella extract according to the established extraction process, dry the extracted chlorella extract in a constant temperature drying oven at the set temperature to constant weight, weigh the mass of the chlorella extract after drying (m2), and calculate the extract yield according to the formula: extract yield (%) = (m2 / m1) × 100%.

[0043] 2. Polysaccharide content test: Prepare a series of polysaccharide standard solutions of known concentrations ranging from 0.1 mg / mL to 1 mg / mL. Add a predetermined amount of phenol solution (5% phenol solution, mixed in a 1:5 volume ratio) to each standard solution. Then, quickly add sulfuric acid (concentrated sulfuric acid, mixed in a 1:5 volume ratio), mix thoroughly, and react at 30°C for 15 minutes. Measure the absorbance of each standard solution using a spectrophotometer at a wavelength of 490 nm. Plot a standard curve with polysaccharide concentration as the horizontal axis and absorbance as the vertical axis. Perform the same treatment on the Chlorella extract, measure its absorbance, and calculate the polysaccharide content in the Chlorella extract based on the standard curve.

[0044] 3. Protein content test: Prepare protein standard solutions of varying concentrations, ranging from 0.1 mg / mL to 2 mg / mL. Take each standard solution and add Biuret Reagent Solution A (2% sodium hydroxide solution) and Solution B (0.1% copper sulfate solution) in a 10:1:1 volume ratio. Mix thoroughly and allow to react for 10 minutes. Measure the absorbance of each standard solution at 540 nm using a spectrophotometer to create a standard curve. Prepare a sample of Chlorella extract using the same procedure, measure the absorbance, and calculate the protein content based on the standard curve.

[0045] 4. CGF content test: The target component, RNA, in CGF was extracted and purified to obtain a sample solution. The standard solution and sample solution were analyzed separately using high-performance liquid chromatography (HPLC) under the following conditions: mobile phase: 0.1% aqueous phosphoric acid (phase A) and acetonitrile (phase B); gradient elution program: 0-10 minutes, 10%-20% phase B; 10-20 minutes, 20%-30% phase B; 20-30 minutes, 30%-40% phase B; detection wavelength: 210 nm; flow rate: 1.0 mL / min; column temperature: 30°C. The standard solution and sample solution were analyzed separately, and the chromatographic peak of the target component was identified based on its retention time. The content of the target CGF component in the sample was calculated by comparing the peak area or peak height with that of the standard.

[0046] 5. Moisturizing rate test: Recruit 30 volunteers with healthy skin (aged 18-60, no skin diseases). Cleanse and dry the test area (inner forearm or cheek). Measure the initial skin moisture content (W1) of each volunteer's test area using a skin moisture meter. Apply an appropriate amount of skin care composition (thickness approximately 2 mg / cm) to the test area. 2 ), after the prescribed time interval (24 hours), use the skin moisture tester to measure the skin moisture content (W2) of the area again.

[0047] Moisturizing rate (%) = [(W2-W1) / W1] × 100%.

[0048] 6. DPPH free radical scavenging rate: Prepare a certain concentration of DPPH ethanol solution (0.1mmol / L) and store it in a dark environment. Take an appropriate amount of skin care composition sample and dilute it with anhydrous ethanol to different concentration gradients (1mg / mL, 5mg / mL, 10mg / mL). Take each concentration sample solution and mix it with DPPH solution in a volume ratio of 1:1, and react for 30 minutes in the dark. Use a spectrophotometer to measure the absorbance value (A) of the reaction mixture at a specific wavelength (517nm). 样 ) and blank control (A 空 , with anhydrous ethanol instead of sample solution) and DPPH reagent blank (A 试剂 , using anhydrous ethanol instead of DPPH solution) absorbance value.

[0049] DPPH free radical scavenging rate (%) = [1-(A 样 -A 试剂 ) / A 空 ]×100%.

[0050] 7. Cell proliferation rate: Human skin fibroblasts (HSFs) were cultured in vitro in DMEM high-glucose medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin in a 37°C, 5% CO2 incubator. After the cells reached a certain density (approximately 80% confluency), 100 μL of the cell suspension was seeded into each well of a 96-well culture plate and allowed to adhere to the plate. After the cells stabilized (approximately 24 hours of culture), the skin care composition was added to the culture system at varying concentrations (e.g., 0.1 mg / mL, 0.5 mg / mL, 1 mg / mL) and a control group (no skin care composition) was added. Six replicate wells were set up for each treatment. Culture was continued for 24 hours, after which the cells in each well were digested, collected by centrifugation, and resuspended in saline to prepare a cell suspension.

[0051] Take an appropriate amount of cell suspension and place it on a cell counting plate, and count the number of cells under a microscope.

[0052] Cell proliferation rate (%) = [(number of cells in the experimental group - number of cells in the control group) / number of cells in the control group] × 100%.

[0053] 8. Centrifugal stability: Take an appropriate amount of skin care composition sample (about 10 mL), put it into a centrifuge tube, and record the initial state of the sample. Place the centrifuge tube in a centrifuge, set the speed to 3000 r / min, and centrifuge for 15 minutes. After the centrifugation is completed, immediately observe the stratification of the sample in the centrifuge tube and record the stratification phenomenon (such as whether there is obvious stratification, the height of the stratification, etc.). If the sample does not stratify after centrifugation, it is judged that the centrifugal stability is good; if stratification occurs, continue to observe the static stability after stratification, record the time required for the stratified part to be re-mixed evenly or the time index for the stratified part to exist stably, so as to evaluate the centrifugal stability of the skin care composition. Analysis of the test results of each embodiment and comparative example The performance test results of the Chlorella extracts and skin care compositions prepared in Examples 1 to 3 and Comparative Examples 1 to 5 are shown in Table 3-1 and Table 3-2.

[0054] Table 3-1

[0055] Table 3-2

[0056] The data in Table 3 demonstrate that the mixed Chlorella extract prepared in Example 1 has a higher nutritional value, and the resulting skin care composition exhibits superior moisturizing, antioxidant, and repair effects, as well as improved stability. The data from Comparative Example 1 and Example 1 demonstrate that excessive amounts of Chlorella pyrenoidosa disrupt the balance and synergistic effects of the various components in the mixed Chlorella extract, altering the extract's physicochemical properties and ultimately reducing the overall nutritional value, moisturizing, antioxidant, repair, and stability of the extract and skin care composition.

[0057] Comparison of the data of Comparative Examples 2, 3, and 4 with that of Example 1 shows that the effect of a single species of Chlorella is poor.

[0058] Compared with Example 1, Comparative Example 5 shows that after replacing Chlorella pyrenoidosa with Spirulina, the performance is slightly inferior to that of Example 1. After replacing Chlorella pyrenoidosa with Spirulina, due to the differences in polysaccharide content, types of unsaturated fatty acids, antioxidant components, etc. between Spirulina and Chlorella pyrenoidosa, Chlorella vulgaris and Chlorella ellipsoidea, the component differences and synergistic effects are destroyed, resulting in its overall performance being slightly inferior to that of Example 1.

[0059] It should be noted that the present invention is not limited to the above-mentioned embodiments. The above-mentioned embodiments are merely examples, and any embodiments having substantially the same structure and effect as the technical concept within the scope of the technical solution of the present invention are all included in the technical scope of the present invention. In addition, without departing from the scope of the present invention, other embodiments that can be conceived by those skilled in the art and that combine some of the constituent elements in the embodiments are also included in the scope of the present invention.

Claims

1. A skin care composition based on mixed chlorella extract, characterized in that, The raw material components include the following by weight percentage: mixed chlorella extract 7% to 15%, synergist 4% to 10%, moisturizer 12% to 30%, emulsifier 2.0% to 8.0%, thickener 0.3% to 0.6%, preservative 0.5% to 1.2%, fragrance 0.01% to 0.4% and the balance deionized water; The mixed chlorella extract is obtained by extracting Chlorella pyrenoidosa, Chlorella vulgaris and Chlorella ellipsoidea as raw materials.

2. A skin care composition based on mixed chlorella extract according to claim 1, characterized in that: The mass ratio of Chlorella pyrenoidosa, Chlorella vulgaris and Chlorella ellipsoidea is 1:(0.9-1.1):(0.9-1.1).

3. The skin care composition based on mixed chlorella extract according to claim 1, characterized in that: The synergist includes at least one of vitamin C, vitamin E, astaxanthin, and asiaticoside; and / or the moisturizer includes at least one of sodium hyaluronate, ceramide, butylene glycol, and 1,2-hexanediol.

4. The skin care composition based on mixed chlorella extract according to claim 1, characterized in that: The emulsifier includes at least one of sucrose laurate, behenic acid, cetearyl alcohol, cholesterol, and polyglyceryl-10 stearate; and / or the thickener includes at least one of carbomer, xanthan gum, polyacrylate crosspolymer-6, and guar gum.

5. The skin care composition based on mixed chlorella extract according to claim 1, characterized in that: The preservative is selected from a mixture of two or more of potassium sorbate, sodium benzoate, ethylhexylglycerin, and caprylyl glycol; and / or the fragrance includes at least one of lavender essential oil, chamomile essential oil, tea tree essential oil, benzoate fragrance, and ethyl maltol.

6. The method for preparing the skin care composition based on mixed Chlorella extract according to any one of claims 1 to 5, wherein: The following steps are involved: The chlorella pyrenoidosa, chlorella vulgaris and chlorella ellipsoidea are mixed, dried and crushed, the crushed chlorella is mixed with ethanol, extracted by ultrasonic-assisted extraction, and centrifuged to obtain a chlorella extract; The chlorella extract is mixed with a synergist, a moisturizer, an emulsifier, a thickener, a preservative, a fragrance and deionized water, and then stirred evenly to obtain the skin care composition based on the mixed chlorella extract.

7. The method for preparing the skin care composition based on mixed Chlorella extract according to claim 6, characterized in that: The drying method includes microwave drying; and / or the drying temperature is 50-70° C., and the drying time is 2-4 hours.

8. The method for preparing the skin care composition based on mixed Chlorella extract according to claim 6, characterized in that: The material-liquid ratio of the crushed Chlorella to the ethanol is 1 g: (10-20) mL.

9. The method for preparing the skin care composition based on mixed Chlorella extract according to claim 6, characterized in that: The conditions of the ultrasonic-assisted extraction method are as follows: power 200-800W, time 5-30 minutes.

10. Use of the skin care composition based on mixed Chlorella extract according to any one of claims 1 to 5 in the preparation of cosmetics.

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